TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5847
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5847
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s disease (AD) is crucial due to the lack of effective management and treatment modalities. Higher prevalence, progression rate and severity of AD in women than in men also establish sex as key variable in AD therapy development.Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to nine-fold higher prevalence in women compared to men. The molecular mechanisms by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain however elusive. We therefore examined sex-related alterations in gene expression of iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA level was evident in female WT mice, while a trend toward a decrease was detected in their APPNL-G-F KI littermates. Expression in the opposite direction was observed for TTR, with a robust genotype-dependent decrease in male mice.Results are in line with well-established role of THs in the regulation of neuronal plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5847"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5847 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Perović, Milka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5846
AB  - Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Thyroid hormone metabolism in the cortex of male and female APP knock-in mice
SP  - 111
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5846
ER  - 

@conference{
author = "Ćirić, Jelena and Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Filipović, Branko and Šošić-Jurjević, Branka  and Perović, Milka",
year = "2023",
abstract = "Targeting novel pathways contributing to the pathogenesis/progression of Alzheimer’s 
disease (AD) is crucial due to the lack of effective management and treatment 
modalities. Higher prevalence, progression rate and severity of AD in women than in 
men also establish sex as key variable in AD therapy development.
Thyroid disorders, both hyper- and hypothyroidism, were found to occur with up to 
nine-fold higher prevalence in women compared to men. The molecular mechanisms 
by which thyroid dysfunction contribute to AD pathogenesis and heterogeneity remain 
however elusive. We therefore examined sex-related alterations in gene expression of 
iodothyronine deiodinase 2 (Dio2) and transthyretin (TTR) involved in the tissue 
metabolism and the distribution of thyroid hormones (THs), respectively, in a novel, 
state-of the art knock-in (KI) mouse model of AD-like amyloidosis, APPNL-G-F mice.
Quantitative RT-PCR analysis revealed prominent differences in cortical Dio2 and 
TTR gene expression in 9-month-old male and female APPNL-G-F mice and their non KI littermates (WT). In comparison to WT male mice, the increase in Dio2 mRNA 
level was evident in female WT mice, while a trend toward a decrease was detected in 
their APPNL-G-F KI littermates. Expression in the opposite direction was observed for 
TTR, with a robust genotype-dependent decrease in male mice.
Results are in line with well-established role of THs in the regulation of neuronal 
plasticity in the adult brain and suggest profound sex-biased effects of TH on Aβ induced pathology in APPNL-G-F mice.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice",
pages = "111",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5846"
}

Ćirić, J., Milovanović, N., Jovanović Macura, I., Tešić, V., Filipović, B., Šošić-Jurjević, B.,& Perović, M.. (2023). Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846

Ćirić J, Milovanović N, Jovanović Macura I, Tešić V, Filipović B, Šošić-Jurjević B, Perović M. Thyroid hormone metabolism in the cortex of male and female APP knock-in mice. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:111.
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

Ćirić, Jelena, Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Filipović, Branko, Šošić-Jurjević, Branka , Perović, Milka, "Thyroid hormone metabolism in the cortex of male and female APP knock-in mice" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):111,
https://hdl.handle.net/21.15107/rcub_ibiss_5846 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5842
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5842
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5842"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):110,
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5843
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5843
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5843"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5843

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

TY  - JOUR
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Ivković, Sanja
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9599456
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5172
AB  - Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.
PB  - Basel: MDPI
T2  - Brain Sciences
T1  - Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging
IS  - 10
VL  - 12
DO  - 10.3390/brainsci12101297
SP  - 1297
ER  - 

@article{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Ivković, Sanja and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Glucocorticoids are the most potent anti-inflammatory agents known. Limited in vivo data are available to characterize the mechanism underlying their cognitive side effects and transient occurrence of steroid psychosis. Cholesterol is important for proper neurotransmission and brain plasticity, and disruption of its homeostasis in the brain has been closely associated with memory decline during aging and in age-related neurodegenerative disorders. In the present study, we assessed the direct effects of dexamethasone, a potent synthetic glucocorticoid, on the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGCR), apolipoprotein E (ApoE) and cholesterol 24S-hydroxylase (CYP46A1), major enzymes involved in cholesterol synthesis, metabolism, and excretion, respectively. The effects of the dexamethasone were examined during aging, in the cortex and hippocampus of 6-, 12- and 18-month-old rats, and following long-term food restriction (FR). The most prominent change observed was the age-related decrease in ApoE mRNA regardless of the food regimen applied. In animals kept on FR, this decrease was accompanied by an increase in the mRNA expression of HMGCR and CYP46A1. The present study also demonstrates that food restriction reversed most of the dexamethasone-induced changes in the expression of genes involved in regulation of cholesterol homeostasis in aging rats, in a region-specific manner.",
publisher = "Basel: MDPI",
journal = "Brain Sciences",
title = "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging",
number = "10",
volume = "12",
doi = "10.3390/brainsci12101297",
pages = "1297"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Ivković, S., Kanazir, S.,& Perović, M.. (2022). Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences
Basel: MDPI., 12(10), 1297.
https://doi.org/10.3390/brainsci12101297

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Ivković S, Kanazir S, Perović M. Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging. in Brain Sciences. 2022;12(10):1297.
doi:10.3390/brainsci12101297 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Ivković, Sanja, Kanazir, Selma, Perović, Milka, "Food Restriction Counteracts Dexamethasone-Induced Downregulation of Genes Involved in Cholesterol Homeostasis in Rat Brain during Aging" in Brain Sciences, 12, no. 10 (2022):1297,
https://doi.org/10.3390/brainsci12101297 . .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pešić, Vesna
AU  - Hofman, Katarina
AU  - Perović, Milka
AU  - Ćirić, Jelena
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5616
AB  - Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење које 
карактеришу бројни когнитивни поремећаји и промене у  понашању. Главни је 
узрок деменције код старих особа, а услед пораста просечне старости популације, 
очекује се и да ће број оболелих бити дуплиран у следеће две деценије. У циљу 
разумевања патолошких механизама у основи овог обољења, као и испитивања 
потенцијалних терапијских приступа, развијен је велики број трансгених мишјих 
модела АБ. APPNL-G-F мишеви су „knock-in“ модел АБ новије генерације које 
карактерише присуство хуманог амилоидног прекурсорског протеина (АРР) са три 
мутације које се јављају у  фамилијарном облику болести. Модел карактерише 
убрзан ток патолошких промена, односно депоновање амилоида и појава глиозе 
већ код животиња старих 2 месеца. Да би се окарактерисале ране промене у 
понашању, код мужјака APPNL-G-F мишева  старих 7 месеци и њихових контрола 
дивљег типа из истих окота, испитиване су локомоторна активност и краткотрајна 
меморија. Локомоторна активност је испитивана Тестом отвореног поља, док је 
краткотрајна меморија испитивана Тестом препознавања новог објекта. Додатно, 
један од објеката у тесту отвореног поља је накнадно померан, чинећи тест 
погодним за испитивање краткотрајне визуелне и просторне меморије. Резултати 
су указали на значајне промене у понашању које могу послужити као рани маркери 
когнитивних дефицита у овом моделу.
AB  - Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje koje karakterišu brojni kognitivni poremećaji i promene u ponašanju. Glavni je uzrok demencije kod starih osoba, a usled porasta prosečne starosti populacije, očekuje se i da će broj obolelih biti dupliran u sledeće dve decenije. U cilju razumevanja patoloških mehanizama u osnovi ovog oboljenja, kao i ispitivanja potencijalnih terapijskih pristupa, razvijen je veliki broj transgenih mišjih modela AB. APPNL-G-F miševi su „knock-in“ model AB novije generacije koje karakteriše prisustvo humanog amiloidnog prekursorskog proteina (ARR) sa tri mutacije koje se javljaju u familijarnom obliku bolesti. Model karakteriše ubrzan tok patoloških promena, odnosno deponovanje amiloida i pojava glioze već kod životinja starih 2 meseca. Da bi se okarakterisale rane promene u ponašanju, kod mužjaka APPNL-G-F miševa starih 7 meseci i njihovih kontrola divljeg tipa iz istih okota, ispitivane su lokomotorna aktivnost i kratkotrajna memorija. Lokomotorna aktivnost je ispitivana Testom otvorenog polja, dok je kratkotrajna memorija ispitivana Testom prepoznavanja novog objekta. Dodatno, jedan od objekata u testu otvorenog polja je naknadno pomeran, čineći test pogodnim za ispitivanje kratkotrajne vizuelne i prostorne memorije. Rezultati su ukazali na značajne promene u ponašanju koje mogu poslužiti kao rani markeri kognitivnih deficita u ovom modelu.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti
T1  - Карактеризација локомоторне активности и краткотрајне меморије APPNL-G-F мишева као анималног модела Алцхајмерове болести
SP  - 382
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5616
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pešić, Vesna and Hofman, Katarina and Perović, Milka and Ćirić, Jelena",
year = "2022",
abstract = "Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење које 
карактеришу бројни когнитивни поремећаји и промене у  понашању. Главни је 
узрок деменције код старих особа, а услед пораста просечне старости популације, 
очекује се и да ће број оболелих бити дуплиран у следеће две деценије. У циљу 
разумевања патолошких механизама у основи овог обољења, као и испитивања 
потенцијалних терапијских приступа, развијен је велики број трансгених мишјих 
модела АБ. APPNL-G-F мишеви су „knock-in“ модел АБ новије генерације које 
карактерише присуство хуманог амилоидног прекурсорског протеина (АРР) са три 
мутације које се јављају у  фамилијарном облику болести. Модел карактерише 
убрзан ток патолошких промена, односно депоновање амилоида и појава глиозе 
већ код животиња старих 2 месеца. Да би се окарактерисале ране промене у 
понашању, код мужјака APPNL-G-F мишева  старих 7 месеци и њихових контрола 
дивљег типа из истих окота, испитиване су локомоторна активност и краткотрајна 
меморија. Локомоторна активност је испитивана Тестом отвореног поља, док је 
краткотрајна меморија испитивана Тестом препознавања новог објекта. Додатно, 
један од објеката у тесту отвореног поља је накнадно померан, чинећи тест 
погодним за испитивање краткотрајне визуелне и просторне меморије. Резултати 
су указали на значајне промене у понашању које могу послужити као рани маркери 
когнитивних дефицита у овом моделу., Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje koje karakterišu brojni kognitivni poremećaji i promene u ponašanju. Glavni je uzrok demencije kod starih osoba, a usled porasta prosečne starosti populacije, očekuje se i da će broj obolelih biti dupliran u sledeće dve decenije. U cilju razumevanja patoloških mehanizama u osnovi ovog oboljenja, kao i ispitivanja potencijalnih terapijskih pristupa, razvijen je veliki broj transgenih mišjih modela AB. APPNL-G-F miševi su „knock-in“ model AB novije generacije koje karakteriše prisustvo humanog amiloidnog prekursorskog proteina (ARR) sa tri mutacije koje se javljaju u familijarnom obliku bolesti. Model karakteriše ubrzan tok patoloških promena, odnosno deponovanje amiloida i pojava glioze već kod životinja starih 2 meseca. Da bi se okarakterisale rane promene u ponašanju, kod mužjaka APPNL-G-F miševa starih 7 meseci i njihovih kontrola divljeg tipa iz istih okota, ispitivane su lokomotorna aktivnost i kratkotrajna memorija. Lokomotorna aktivnost je ispitivana Testom otvorenog polja, dok je kratkotrajna memorija ispitivana Testom prepoznavanja novog objekta. Dodatno, jedan od objekata u testu otvorenog polja je naknadno pomeran, čineći test pogodnim za ispitivanje kratkotrajne vizuelne i prostorne memorije. Rezultati su ukazali na značajne promene u ponašanju koje mogu poslužiti kao rani markeri kognitivnih deficita u ovom modelu.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti, Карактеризација локомоторне активности и краткотрајне меморије APPNL-G-F мишева као анималног модела Алцхајмерове болести",
pages = "382",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5616"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pešić, V., Hofman, K., Perović, M.,& Ćirić, J.. (2022). Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 382.
https://hdl.handle.net/21.15107/rcub_ibiss_5616

Milovanović N, Jovanović Macura I, Tešić V, Pešić V, Hofman K, Perović M, Ćirić J. Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:382.
https://hdl.handle.net/21.15107/rcub_ibiss_5616 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pešić, Vesna, Hofman, Katarina, Perović, Milka, Ćirić, Jelena, "Karakterizacija lokomotorne aktivnosti i kratkotrajne memorije APPNL-G-F miševa kao animalnog modela Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):382,
https://hdl.handle.net/21.15107/rcub_ibiss_5616 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Hofman, Katarina
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5613
AB  - Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа.
AB  - Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti
T1  - Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести
SP  - 343
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5613
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Hofman, Katarina and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Дуготрајна рестрикција уноса хране повољно делује на организам у целини, а 
показанa су и бројна неуропротективна дејства овог режима исхране на обољења 
повезана са старењем, попут Алцхајмерове болести. Циљ ове студије је био да се 
испита превентивно дејство рестрикције уноса хране и утицај на парвалбуминске 
неуроне (PV) коре великог мозга и BDNF/ТrkB сигнални пут у трансгеном моделу 
Алцхајмерове болести. Женке 5XFAD мишева и њихове не-трансгене контроле су 
биле излагане ad libidum (AL) или EOD (од енгл. Every-Other-Day feeding) режиму 
исхране почевши од другог месеца старости. Број PV неурона је одређиван 
имунохистохемијском методом у  retrosplenial dysgranular cortex  (RSD), 
retrosplenial granular cortex  (RSG),  parietal cortex  (PtA) и  somatosensory  cortex  (S) 
код животиња старих шест месеци. Код TgAL мишева је утврђено значајно 
смањење броја PV неурона у RSGc, PtA и S, док промене у броју у RSD нису 
уочене. Четири месеца ЕОD режима исхране је смањило пад у броју PV неурона у 
сва три испитивана региона. Анализа BDNF/ТrkB сигналног пута имуноблот 
поступком је такође указала на смањење BDNF-а код TgAL мишева, али и на 
додатно смањење овог протеина код TgЕОD мишева. Значајне разлике у pro-
BDNF-у (прекурсор  BDNF-а)  нису уочене. Резултати ове студије указују да 
рестрикција хране може спречити губитак PV неурона у трансгеном моделу 
Алцхајмерове болести, што доприноси и бољем разумевању неуронске  основе 
когнитивних поремећаја у овом обољењу и од значаја је за даљи развој потребних 
додатних терапијских приступа., Dugotrajna restrikcija unosa hrane povoljno deluje na organizam u celini, a pokazana su i brojna neuroprotektivna dejstva ovog režima ishrane na oboljenja povezana sa starenjem, poput Alchajmerove bolesti. Cilj ove studije je bio da se ispita preventivno dejstvo restrikcije unosa hrane i uticaj na parvalbuminske neurone (PV) kore velikog mozga i BDNF/TrkB signalni put u transgenom modelu Alchajmerove bolesti. Ženke 5XFAD miševa i njihove ne-transgene kontrole su bile izlagane ad libidum (AL) ili EOD (od engl. Every-Other-Day feeding) režimu ishrane počevši od drugog meseca starosti. Broj PV neurona je određivan imunohistohemijskom metodom u retrosplenial dysgranular cortex (RSD), retrosplenial granular cortex (RSG), parietal cortex (PtA) i somatosensory cortex (S) kod životinja starih šest meseci. Kod TgAL miševa je utvrđeno značajno smanjenje broja PV neurona u RSGc, PtA i S, dok promene u broju u RSD nisu uočene. Četiri meseca EOD režima ishrane je smanjilo pad u broju PV neurona u sva tri ispitivana regiona. Analiza BDNF/TrkB signalnog puta imunoblot postupkom je takođe ukazala na smanjenje BDNF-a kod TgAL miševa, ali i na dodatno smanjenje ovog proteina kod TgEOD miševa. Značajne razlike u pro- BDNF-u (prekursor BDNF-a) nisu uočene. Rezultati ove studije ukazuju da restrikcija hrane može sprečiti gubitak PV neurona u transgenom modelu Alchajmerove bolesti, što doprinosi i boljem razumevanju neuronske osnove kognitivnih poremećaja u ovom oboljenju i od značaja je za dalji razvoj potrebnih dodatnih terapijskih pristupa.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti, Дејство рестрикције хране на парвалбуминске неуроне коре великог мозга у трансгеном моделу Алцхајмерове болести",
pages = "343",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5613"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Hofman, K., Kanazir, S.,& Perović, M.. (2022). Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Hofman K, Kanazir S, Perović M. Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:343.
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Hofman, Katarina, Kanazir, Selma, Perović, Milka, "Dejstvo restrikcije hrane na parvalbuminske neurone kore velikog mozga u transgenom modelu Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):343,
https://hdl.handle.net/21.15107/rcub_ibiss_5613 .

TY  - CONF
AU  - Ćirić, Jelena
AU  - Tešić, Vesna
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Kanazir, Selma
AU  - Perović, Milka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5187
AB  - Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice
DO  - 10.1002/2211-5463.13440
SP  - 132
ER  - 

@conference{
author = "Ćirić, Jelena and Tešić, Vesna and Milovanović, Nikola and Jovanović Macura, Irena and Kanazir, Selma and Perović, Milka",
year = "2022",
abstract = "Neuroprotective effects of food restriction were demonstrated in
several animal models of stroke, traumatic brain injury, and neurodegenerative
diseases. Since Alzheimer’s disease (AD) is characterized
by a long silent prodromal phase, the present study
aimed to determine the effects of every-other-day (EOD) feeding
on cortical responsiveness to PV interneurons and BDNF/TrkB
signaling using 5xFAD mice, a well-characterized mouse model
of AD. Female 5xFAD transgenic (Tg) mice and their non-transgenic
littermates were exposed to ad libitum (AL) or EOD feeding
regimen, beginning at 2 months of age. Neurons expressing
PV were detected in the retrosplenial dysgranular cortex (RSGc),
retrosplenial granular cortex (RSD), parietal cortex (PtA), and
somatosensory cortex (S) of 6-month-old animals by immunohistochemistry.
Analysis of the BDNF/Trk signaling was examined
by western blot. TgAL mice showed a significantly reduced number
of PV-positive cells in the RSGc, PtA, and S, while no
changes were detected in the RSD. Interestingly, four months of
EOD feeding reverted the number of PV-positive cells to control
values in all three regions examined. BDNF was decreased in the
TgAL mice, which was additionally decreased in TgEOD mice,
while no significant difference in pro-BDNF was identified. Analysis
of TrkB and pTrkB revealed a significant increase of TrkB
in the TgEOD group, whereas a significant decrease in pTrkB
was detected only in the TgAL group. The present study indicates
that every-other-day feeding can ameliorate PV neuronal
loss, and have an important role in further understanding the
neural basis of AD-like-associated cognitive impairments in
5xFAD mouse model of AD.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice",
doi = "10.1002/2211-5463.13440",
pages = "132"
}

Ćirić, J., Tešić, V., Milovanović, N., Jovanović Macura, I., Kanazir, S.,& Perović, M.. (2022). Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 132.
https://doi.org/10.1002/2211-5463.13440

Ćirić J, Tešić V, Milovanović N, Jovanović Macura I, Kanazir S, Perović M. Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:132.
doi:10.1002/2211-5463.13440 .

Ćirić, Jelena, Tešić, Vesna, Milovanović, Nikola, Jovanović Macura, Irena, Kanazir, Selma, Perović, Milka, "Every-other-day feeding affects the number of parvalbumin-expressing neurons and BDNF/Trk signaling in the cortex of 5xFAD mice" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):132,
https://doi.org/10.1002/2211-5463.13440 . .