TY  - CONF
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6227
AB  - Introduction: Global warming profoundly impacts ecological communities as higher temperatures affects growth and metabolism of individuals, altering their survival and reproductive success. To anticipate
plant response to future temperature increase, two natural populations of Iris pumila inhabiting sun-exposed environment were subjected to an open-top chamber (OTC) experiment and investigated for the
amount of Hsp17.6.
Methods: One half of all randomly selected circle-shaped clones of I. pumila were experimentally
warmed, by 1-2°C, using clear-sided OTC, while the other half was exposed to ambient temperature conditions. In spring and summer, over a two-year period, ramets of each clone growing inside and outside
of OTC were analyzed for the Hsp17.6 content.
Results: Immunoblot analysis revealed the presence of two Hsp17.6 isoforms, whose quantities were
greater in ramets growing inside the OTCs than in those growing outside. The mean response profiles of
both protein isoforms were parallel over time and the total amount of Hsp17.6 reached its maximum in
the summer. A repeated-measures profile analysis revealed significant treatment and season effect for
both Hsp17.6 isoforms, whereas year effect was significant only for the higher molecular weight isoform.
Furthermore, profile analysis of the between-population effects showed that the mean response profiles, for both isoforms, differ between populations.
Conclusion: A small temperature increase can alter both the level and shape of the mean response profiles of Hsp17.6 in I. pumila, suggesting the species’capability to acclimate to increasing temperatures by
plastic response of small heat shock proteins, the plants’ key molecular chaperones associated with enhanced thermotolerance.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Plastic response of Iris pumila small heat shock protein Hsp17.6 to experimental warming in situ
SP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6227
ER  - 

@conference{
author = "Hočevar, Katarina and Vuleta, Ana and Manitašević Jovanović, Sanja",
year = "2023",
abstract = "Introduction: Global warming profoundly impacts ecological communities as higher temperatures affects growth and metabolism of individuals, altering their survival and reproductive success. To anticipate
plant response to future temperature increase, two natural populations of Iris pumila inhabiting sun-exposed environment were subjected to an open-top chamber (OTC) experiment and investigated for the
amount of Hsp17.6.
Methods: One half of all randomly selected circle-shaped clones of I. pumila were experimentally
warmed, by 1-2°C, using clear-sided OTC, while the other half was exposed to ambient temperature conditions. In spring and summer, over a two-year period, ramets of each clone growing inside and outside
of OTC were analyzed for the Hsp17.6 content.
Results: Immunoblot analysis revealed the presence of two Hsp17.6 isoforms, whose quantities were
greater in ramets growing inside the OTCs than in those growing outside. The mean response profiles of
both protein isoforms were parallel over time and the total amount of Hsp17.6 reached its maximum in
the summer. A repeated-measures profile analysis revealed significant treatment and season effect for
both Hsp17.6 isoforms, whereas year effect was significant only for the higher molecular weight isoform.
Furthermore, profile analysis of the between-population effects showed that the mean response profiles, for both isoforms, differ between populations.
Conclusion: A small temperature increase can alter both the level and shape of the mean response profiles of Hsp17.6 in I. pumila, suggesting the species’capability to acclimate to increasing temperatures by
plastic response of small heat shock proteins, the plants’ key molecular chaperones associated with enhanced thermotolerance.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Plastic response of Iris pumila small heat shock protein Hsp17.6 to experimental warming in situ",
pages = "142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6227"
}

Hočevar, K., Vuleta, A.,& Manitašević Jovanović, S.. (2023). Plastic response of Iris pumila small heat shock protein Hsp17.6 to experimental warming in situ. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 142.
https://hdl.handle.net/21.15107/rcub_ibiss_6227

Hočevar K, Vuleta A, Manitašević Jovanović S. Plastic response of Iris pumila small heat shock protein Hsp17.6 to experimental warming in situ. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:142.
https://hdl.handle.net/21.15107/rcub_ibiss_6227 .

Hočevar, Katarina, Vuleta, Ana, Manitašević Jovanović, Sanja, "Plastic response of Iris pumila small heat shock protein Hsp17.6 to experimental warming in situ" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):142,
https://hdl.handle.net/21.15107/rcub_ibiss_6227 .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5892
AB  - Temperature is considered as the most ecologically relevant environmental factor since it affects all biological processes within an organism. It impacts the physiological responses, fitness and the distribution of organisms. Therefore it is expected that global warming will significantly alter biodiversity and consequently influence ecosystem processes. Plant functional traits are morpho-physio-phenological characteristics that shape plant performance through their effects on growth, reproduction, and survival, underlie vegetation responses to environmental changes. To anticipate the effects of global warming on plant functioning, we assessed the phenotypic expression of the key leaf functional trait – specific leaf area (SLA), in response to a temperature increase. For that purpose, Iris pumila plants developed from rhizome segments of clonal genotypes originated from dune and wood natural populations of Deliblato Sands and commonly cultivated in a growth room at 23/19°C (day/night) ambient temperature, were subjected to experimental warming. To simulate lower and upper projected warming regimes expected within the current century, a total of 46 clonal genotypes were first warmed by 1°C (24/20°C day/night) and then by 4°C (27/23°C day/night). Within each temperature treatment, the last fully developed leaf was harvested from each plant and analyzed for the SLA. The mean SLA value was found to vary both across temperature treatments and between populations. The repeated-measures profile analysis revealed that both populations responded in the same manner but with different magnitudes. In both populations, SLA increased with temperature, but those values were significantly greater in the dune genotypes than in the wood ones. These findings suggest that I. pumila has the ability to counteract rising temperatures through the plastic response of SLA and that the level of this response is likely to be dependent on the habitat type I. pumila plants derived from.
AB  - Temperatura se smatra abiotičkim faktorom sa najvažnijim ekološkim značajem, jer deluje na sve biološke procese u organizmu. Ona utiče na fiziološke odgovore, adapativnu vrednost, i prostornu rasprostranjenost organizama. Zbog toga se očekuje da će globalno zagrevanje značajno uticati na biodiverzitet i, posledično, dovesti do promena u funkcionisanju ekosistema. Funkcionalne osobine biljaka su morfološko-fiziološko-fenološke karakteristike koje oblikuju performansu biljaka utičući na njihov rast, reprodukciju i preživljavanje, i nalaze se u osnovi odgovora biljnih zajednica na promene životne sredine. Kako bi se predvideli efekti globalnog zagrevanja na funkcionisanje biljaka, procenjena je fenotipska ekspresija najvažnije funcionalne osobine lista, specifične površine lista - SLA (eng. Specific Leaf Area), u odgovoru na povećanu temperaturu. U tu svrhu, biljke Iris pumila dobijene presađivanjem rizoma klonalnih genotipova poreklom iz dinske i šumske prirodne populacije Deliblatske peščare, a koje su gajene u eksperimentalnoj sobi za rast biljaka na temperaturi od 23/19°C (dan/noć), izložene su eksperimentalnom zagrevanju. Da bi se simulirali niži i viši projektovani režimi zagrevanja koji se očekuju u toku ovog veka, 46 klonalnih genotipova je izloženo porastu ambijentalne temperature od 1°C  (24/20°C dan/noć), a zatim i od 4°C (27/23°C dan/noć). U okviru svakog temperaturnog tretmana, sa svake biljke je uzorkovan poslednji potpuno razvijen list i određena  je SLA. Pokazano je da su srednje vrednosti SLA varirale kako između temperaturnih tretmana tako i između populacija. Analiza profila sa ponovljenim merenjima (eng. repeated-measures profile analysis) je pokazala da su obe populacije odgovorile na isti način, ali sa različitim nivoima odgovora. U obe populacije, vrednosti SLA su se povećavale sa povećanjem temperature, ali su ove vrednosti bile značajno veće u dinskim nego u šumskim genotipovima. Dobijeni rezultati sugerišu da I. pumila ima sposobnost da se odupre povećanju ambijentalne temperature plastičnim odgovorom SLA, kao i da nivo tog odgovora verovatno zavisi od tipa staništa iz kojih biljke potiču.
PB  - Belgrade: Faculty of Forestry, University of Belgrade
C3  - Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia
T1  - Utvrđivanje ekoloških odgovora Iris pumila na globalno zagrevanje
SP  - 191
EP  - 205
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5892
ER  - 

@conference{
author = "Hočevar, Katarina and Vuleta, Ana and Manitašević Jovanović, Sanja",
year = "2023",
abstract = "Temperature is considered as the most ecologically relevant environmental factor since it affects all biological processes within an organism. It impacts the physiological responses, fitness and the distribution of organisms. Therefore it is expected that global warming will significantly alter biodiversity and consequently influence ecosystem processes. Plant functional traits are morpho-physio-phenological characteristics that shape plant performance through their effects on growth, reproduction, and survival, underlie vegetation responses to environmental changes. To anticipate the effects of global warming on plant functioning, we assessed the phenotypic expression of the key leaf functional trait – specific leaf area (SLA), in response to a temperature increase. For that purpose, Iris pumila plants developed from rhizome segments of clonal genotypes originated from dune and wood natural populations of Deliblato Sands and commonly cultivated in a growth room at 23/19°C (day/night) ambient temperature, were subjected to experimental warming. To simulate lower and upper projected warming regimes expected within the current century, a total of 46 clonal genotypes were first warmed by 1°C (24/20°C day/night) and then by 4°C (27/23°C day/night). Within each temperature treatment, the last fully developed leaf was harvested from each plant and analyzed for the SLA. The mean SLA value was found to vary both across temperature treatments and between populations. The repeated-measures profile analysis revealed that both populations responded in the same manner but with different magnitudes. In both populations, SLA increased with temperature, but those values were significantly greater in the dune genotypes than in the wood ones. These findings suggest that I. pumila has the ability to counteract rising temperatures through the plastic response of SLA and that the level of this response is likely to be dependent on the habitat type I. pumila plants derived from., Temperatura se smatra abiotičkim faktorom sa najvažnijim ekološkim značajem, jer deluje na sve biološke procese u organizmu. Ona utiče na fiziološke odgovore, adapativnu vrednost, i prostornu rasprostranjenost organizama. Zbog toga se očekuje da će globalno zagrevanje značajno uticati na biodiverzitet i, posledično, dovesti do promena u funkcionisanju ekosistema. Funkcionalne osobine biljaka su morfološko-fiziološko-fenološke karakteristike koje oblikuju performansu biljaka utičući na njihov rast, reprodukciju i preživljavanje, i nalaze se u osnovi odgovora biljnih zajednica na promene životne sredine. Kako bi se predvideli efekti globalnog zagrevanja na funkcionisanje biljaka, procenjena je fenotipska ekspresija najvažnije funcionalne osobine lista, specifične površine lista - SLA (eng. Specific Leaf Area), u odgovoru na povećanu temperaturu. U tu svrhu, biljke Iris pumila dobijene presađivanjem rizoma klonalnih genotipova poreklom iz dinske i šumske prirodne populacije Deliblatske peščare, a koje su gajene u eksperimentalnoj sobi za rast biljaka na temperaturi od 23/19°C (dan/noć), izložene su eksperimentalnom zagrevanju. Da bi se simulirali niži i viši projektovani režimi zagrevanja koji se očekuju u toku ovog veka, 46 klonalnih genotipova je izloženo porastu ambijentalne temperature od 1°C  (24/20°C dan/noć), a zatim i od 4°C (27/23°C dan/noć). U okviru svakog temperaturnog tretmana, sa svake biljke je uzorkovan poslednji potpuno razvijen list i određena  je SLA. Pokazano je da su srednje vrednosti SLA varirale kako između temperaturnih tretmana tako i između populacija. Analiza profila sa ponovljenim merenjima (eng. repeated-measures profile analysis) je pokazala da su obe populacije odgovorile na isti način, ali sa različitim nivoima odgovora. U obe populacije, vrednosti SLA su se povećavale sa povećanjem temperature, ali su ove vrednosti bile značajno veće u dinskim nego u šumskim genotipovima. Dobijeni rezultati sugerišu da I. pumila ima sposobnost da se odupre povećanju ambijentalne temperature plastičnim odgovorom SLA, kao i da nivo tog odgovora verovatno zavisi od tipa staništa iz kojih biljke potiču.",
publisher = "Belgrade: Faculty of Forestry, University of Belgrade",
journal = "Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia",
title = "Utvrđivanje ekoloških odgovora Iris pumila na globalno zagrevanje",
pages = "191-205",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5892"
}

Hočevar, K., Vuleta, A.,& Manitašević Jovanović, S.. (2023). Utvrđivanje ekoloških odgovora Iris pumila na globalno zagrevanje. in Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia
Belgrade: Faculty of Forestry, University of Belgrade., 191-205.
https://hdl.handle.net/21.15107/rcub_ibiss_5892

Hočevar K, Vuleta A, Manitašević Jovanović S. Utvrđivanje ekoloških odgovora Iris pumila na globalno zagrevanje. in Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia. 2023;:191-205.
https://hdl.handle.net/21.15107/rcub_ibiss_5892 .

Hočevar, Katarina, Vuleta, Ana, Manitašević Jovanović, Sanja, "Utvrđivanje ekoloških odgovora Iris pumila na globalno zagrevanje" in Zbornik radova: Simpozijum sa međunarodnim učešćem: Pejzažna hortikultura 2023: Zdravlje biljaka - zdravlje ljudi; 2023 Feb 9-10; Belgrade, Serbia (2023):191-205,
https://hdl.handle.net/21.15107/rcub_ibiss_5892 .

TY  - JOUR
AU  - Manitašević Jovanović, Sanja
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Tucić, Branka
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6032
AB  - Phenotypic plasticity is widely acknowledged as one of the most common solutions for coping with novel environmental conditions following climate change. However, it is less known whether the current amounts of trait plasticity, which is sufficient for matching with the contemporary climate, will be adequate when global temperatures exceed historical levels. We addressed this issue by exploring the responses of functional and structural leaf traits in Iris pumila clonal individuals to experimentally increased temperatures (~1.5 °C) using an open top chamber (OTC) design. We determined the phenotypic values of the specific leaf area, leaf dry matter content, specific leaf water content, and leaf thickness in the leaves sampled from the same clone inside and outside of the OTC deployed on it, over seasons and years within two natural populations. We analyzed the data using a repeated multivariate analysis of variance, which primarily focusses on the profiles (reaction norms (RNs)) of a variable gathered from the same individual at several different time points. We found that the mean RNs of all analyzed traits were parallel regardless of experienced temperatures, but differed in the level and the shape. The populations RNs were similar as well. As the amount of plasticity in the analyzed leaf trait was adequate for coping with elevated temperatures inside the OTCs, we predict that it will be also sufficient for responding to increased temperatures if they exceed the 1.5 °C target.
PB  - Basel : MDPI
T2  - Plants
T1  - Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L.
IS  - 17
VL  - 12
DO  - 10.3390/plants12173114
SP  - 3114
ER  - 

@article{
author = "Manitašević Jovanović, Sanja and Hočevar, Katarina and Vuleta, Ana and Tucić, Branka",
year = "2023",
abstract = "Phenotypic plasticity is widely acknowledged as one of the most common solutions for coping with novel environmental conditions following climate change. However, it is less known whether the current amounts of trait plasticity, which is sufficient for matching with the contemporary climate, will be adequate when global temperatures exceed historical levels. We addressed this issue by exploring the responses of functional and structural leaf traits in Iris pumila clonal individuals to experimentally increased temperatures (~1.5 °C) using an open top chamber (OTC) design. We determined the phenotypic values of the specific leaf area, leaf dry matter content, specific leaf water content, and leaf thickness in the leaves sampled from the same clone inside and outside of the OTC deployed on it, over seasons and years within two natural populations. We analyzed the data using a repeated multivariate analysis of variance, which primarily focusses on the profiles (reaction norms (RNs)) of a variable gathered from the same individual at several different time points. We found that the mean RNs of all analyzed traits were parallel regardless of experienced temperatures, but differed in the level and the shape. The populations RNs were similar as well. As the amount of plasticity in the analyzed leaf trait was adequate for coping with elevated temperatures inside the OTCs, we predict that it will be also sufficient for responding to increased temperatures if they exceed the 1.5 °C target.",
publisher = "Basel : MDPI",
journal = "Plants",
title = "Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L.",
number = "17",
volume = "12",
doi = "10.3390/plants12173114",
pages = "3114"
}

Manitašević Jovanović, S., Hočevar, K., Vuleta, A.,& Tucić, B.. (2023). Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L.. in Plants
Basel : MDPI., 12(17), 3114.
https://doi.org/10.3390/plants12173114

Manitašević Jovanović S, Hočevar K, Vuleta A, Tucić B. Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L.. in Plants. 2023;12(17):3114.
doi:10.3390/plants12173114 .

Manitašević Jovanović, Sanja, Hočevar, Katarina, Vuleta, Ana, Tucić, Branka, "Predicting the Responses of Functional Leaf Traits to Global Warming: An In Situ Temperature Manipulation Design Using Iris pumila L." in Plants, 12, no. 17 (2023):3114,
https://doi.org/10.3390/plants12173114 . .

TY  - JOUR
AU  - Budečević, Sanja
AU  - Hočevar, Katarina
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5857
AB  - To gain insight into the evolution of flower traits in the generalized food-deceptive plant Iris pumila, we assessed the color, size, shape, and fluctuating asymmetry (FA) of three functionally distinct floral organs—outer perianths (‘falls’), inner perianths (‘standards’), and style branches—and estimated pollinator-mediated selection on these traits. We evaluated the perianth color as the achromatic brightness of the fall, measured the flower stem height, and analyzed the floral organ size, shape, and FA using geometric morphometrics. Pollinated flowers had significantly higher brightness, longer flower stems, and larger floral organs compared to non-pollinated flowers. The shape and FA of the floral organs did not differ, except for the fall FA, where higher values were found for falls of pollinated flowers. Pollinator-mediated selection was confirmed for flower stem height and for subtle changes in the shape of the fall and style branch—organs that form the pollination tunnel. This study provides evidence that, although all analyzed flower traits play significant roles in pollinator attraction, flower stem height and pollination tunnel shape evolved under the pollinator-mediated selection, whereas achromatic brightness, size, and symmetry of floral organs did not directly affect pollination success.
PB  - Basel : MDPI
T2  - Symmetry
T1  - Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators
IS  - 6
VL  - 15
DO  - 10.3390/sym15061149
SP  - 1149
ER  - 

@article{
author = "Budečević, Sanja and Hočevar, Katarina and Manitašević Jovanović, Sanja and Vuleta, Ana",
year = "2023",
abstract = "To gain insight into the evolution of flower traits in the generalized food-deceptive plant Iris pumila, we assessed the color, size, shape, and fluctuating asymmetry (FA) of three functionally distinct floral organs—outer perianths (‘falls’), inner perianths (‘standards’), and style branches—and estimated pollinator-mediated selection on these traits. We evaluated the perianth color as the achromatic brightness of the fall, measured the flower stem height, and analyzed the floral organ size, shape, and FA using geometric morphometrics. Pollinated flowers had significantly higher brightness, longer flower stems, and larger floral organs compared to non-pollinated flowers. The shape and FA of the floral organs did not differ, except for the fall FA, where higher values were found for falls of pollinated flowers. Pollinator-mediated selection was confirmed for flower stem height and for subtle changes in the shape of the fall and style branch—organs that form the pollination tunnel. This study provides evidence that, although all analyzed flower traits play significant roles in pollinator attraction, flower stem height and pollination tunnel shape evolved under the pollinator-mediated selection, whereas achromatic brightness, size, and symmetry of floral organs did not directly affect pollination success.",
publisher = "Basel : MDPI",
journal = "Symmetry",
title = "Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators",
number = "6",
volume = "15",
doi = "10.3390/sym15061149",
pages = "1149"
}

Budečević, S., Hočevar, K., Manitašević Jovanović, S.,& Vuleta, A.. (2023). Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators. in Symmetry
Basel : MDPI., 15(6), 1149.
https://doi.org/10.3390/sym15061149

Budečević S, Hočevar K, Manitašević Jovanović S, Vuleta A. Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators. in Symmetry. 2023;15(6):1149.
doi:10.3390/sym15061149 .

Budečević, Sanja, Hočevar, Katarina, Manitašević Jovanović, Sanja, Vuleta, Ana, "Phenotypic Selection on Flower Traits in Food-Deceptive Plant Iris pumila L.: The Role of Pollinators" in Symmetry, 15, no. 6 (2023):1149,
https://doi.org/10.3390/sym15061149 . .

TY  - JOUR
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Tucić, Branka
AU  - Klingenberg, Christian Peter
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4997
AB  - Directional asymmetry is a systematic difference between the left and right sides for structures with bilateral symmetry or a systematic differentiation among repeated parts for complex symmetry. This study explores factors that produce directional asymmetry in the flower of Iris pumila, a structure with complex symmetry that makes it possible to investigate multiple such factors simultaneously. The shapes and sizes of three types of floral organs, the falls, standards, and style branches, were quantified using the methods of geometric morphometrics. For each flower, this study recorded the compass orientations of floral organs as well as their anatomical orientations relative to the two spathes subtending each flower. To characterize directional asymmetry at the whole-flower level, differences in the average sizes and shapes according to compass orientation and relative orientation were computed, and the left–right asymmetry was also evaluated for each individual organ. No size or shape differences within flowers were found in relation to anatomical position; this may relate to the terminal position of flowers in Iris pumila, suggesting that there may be no adaxial–abaxial polarity, which is very prominent in many other taxa. There was clear directional asymmetry of shape in relation to compass orientation, presumably driven by a consistent environmental gradient such as solar irradiance. There was also clear directional asymmetry between left and right halves of every floral organ, most likely related to the arrangement of organs in the bud. These findings indicate that different factors are acting to produce directional asymmetry at different levels. In conventional analyses not recording flower orientations, these effects would be impossible to disentangle from each other and would probably be included as part of fluctuating asymmetry.
PB  - Hoboken : John Wiley & Sons Ltd
T2  - Evolution & Development
T1  - Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry
DO  - 10.1111/ede.12402
ER  - 

@article{
author = "Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Tucić, Branka and Klingenberg, Christian Peter",
year = "2022",
abstract = "Directional asymmetry is a systematic difference between the left and right sides for structures with bilateral symmetry or a systematic differentiation among repeated parts for complex symmetry. This study explores factors that produce directional asymmetry in the flower of Iris pumila, a structure with complex symmetry that makes it possible to investigate multiple such factors simultaneously. The shapes and sizes of three types of floral organs, the falls, standards, and style branches, were quantified using the methods of geometric morphometrics. For each flower, this study recorded the compass orientations of floral organs as well as their anatomical orientations relative to the two spathes subtending each flower. To characterize directional asymmetry at the whole-flower level, differences in the average sizes and shapes according to compass orientation and relative orientation were computed, and the left–right asymmetry was also evaluated for each individual organ. No size or shape differences within flowers were found in relation to anatomical position; this may relate to the terminal position of flowers in Iris pumila, suggesting that there may be no adaxial–abaxial polarity, which is very prominent in many other taxa. There was clear directional asymmetry of shape in relation to compass orientation, presumably driven by a consistent environmental gradient such as solar irradiance. There was also clear directional asymmetry between left and right halves of every floral organ, most likely related to the arrangement of organs in the bud. These findings indicate that different factors are acting to produce directional asymmetry at different levels. In conventional analyses not recording flower orientations, these effects would be impossible to disentangle from each other and would probably be included as part of fluctuating asymmetry.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "Evolution & Development",
title = "Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry",
doi = "10.1111/ede.12402"
}

Budečević, S., Manitašević Jovanović, S., Vuleta, A., Tucić, B.,& Klingenberg, C. P.. (2022). Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry. in Evolution & Development
Hoboken : John Wiley & Sons Ltd..
https://doi.org/10.1111/ede.12402

Budečević S, Manitašević Jovanović S, Vuleta A, Tucić B, Klingenberg CP. Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry. in Evolution & Development. 2022;.
doi:10.1111/ede.12402 .

Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Tucić, Branka, Klingenberg, Christian Peter, "Directional asymmetry and direction-giving factors: Lessons from flowers with complex symmetry" in Evolution & Development (2022),
https://doi.org/10.1111/ede.12402 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Kostić, Miroslav
AU  - Šešlija Jovanović, Darka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4799
AB  - Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Physiology
T1  - Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say
VL  - 13
DO  - 10.3389/fphys.2022.842314
SP  - 842314
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Vuleta, Ana and Manitašević Jovanović, Sanja and Kostić, Miroslav and Šešlija Jovanović, Darka",
year = "2022",
abstract = "Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Physiology",
title = "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say",
volume = "13",
doi = "10.3389/fphys.2022.842314",
pages = "842314"
}

Lazarević, J., Jevremović, S., Kostić, I., Vuleta, A., Manitašević Jovanović, S., Kostić, M.,& Šešlija Jovanović, D.. (2022). Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology
Lausanne: Frontiers Media SA., 13, 842314.
https://doi.org/10.3389/fphys.2022.842314

Lazarević J, Jevremović S, Kostić I, Vuleta A, Manitašević Jovanović S, Kostić M, Šešlija Jovanović D. Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology. 2022;13:842314.
doi:10.3389/fphys.2022.842314 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Vuleta, Ana, Manitašević Jovanović, Sanja, Kostić, Miroslav, Šešlija Jovanović, Darka, "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say" in Frontiers in Physiology, 13 (2022):842314,
https://doi.org/10.3389/fphys.2022.842314 . .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5047
AB  - Global warming poses a serious threat to the survival of plant populations. To better understand plant responses to future temperature rise, two sun-exposed natural populations of Iris pumila were subjected to an open-top chamber (OTC) experiment over a two-year period. One half of all randomly selected clonal plants were experimentally warmed using clear-sided OTC, while the other half experienced ambient temperature conditions. In general, the air temperature within the OTCs was about 1-2°C higher than that outside of the OTCs. In spring and summer of 2018 and 2019, ramets of each clone growing inside and outside of OTC were analyzed for the amount of Hsp70 and Hsp101. The mean response profiles of both Hsps were parallel over time, but not flat.The amount of both Hsps tended to be greater in ramets growing inside the OTCs than in those growing outside of the OTCs, reaching its maximum in the summer. A repeated-measures profile analysis revealed statistically significant treatment, season and year effect exclusively for the Hsp101, and season effect for the Hsp70. Furthermore, profile analysis of the between-population effects showed that the mean response profiles, for both Hsps, differ between populations. Our results imply that small temperature increase can evoke changes in both the level and shape of the mean response profiles of Hsp70 and Hsp101 in I. pumila compared to that expressed at ambient air temperatures. This finding is especially important to forecast the fate of Iris populations under upcoming climate warming.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Variation in Hsp70 and Hsp101 levels in response to experimental warming in Iris pumila L.: an open-topped chamber experiment
SP  - 138
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5047
ER  - 

@conference{
author = "Hočevar, Katarina and Vuleta, Ana and Manitašević Jovanović, Sanja",
year = "2022",
abstract = "Global warming poses a serious threat to the survival of plant populations. To better understand plant responses to future temperature rise, two sun-exposed natural populations of Iris pumila were subjected to an open-top chamber (OTC) experiment over a two-year period. One half of all randomly selected clonal plants were experimentally warmed using clear-sided OTC, while the other half experienced ambient temperature conditions. In general, the air temperature within the OTCs was about 1-2°C higher than that outside of the OTCs. In spring and summer of 2018 and 2019, ramets of each clone growing inside and outside of OTC were analyzed for the amount of Hsp70 and Hsp101. The mean response profiles of both Hsps were parallel over time, but not flat.The amount of both Hsps tended to be greater in ramets growing inside the OTCs than in those growing outside of the OTCs, reaching its maximum in the summer. A repeated-measures profile analysis revealed statistically significant treatment, season and year effect exclusively for the Hsp101, and season effect for the Hsp70. Furthermore, profile analysis of the between-population effects showed that the mean response profiles, for both Hsps, differ between populations. Our results imply that small temperature increase can evoke changes in both the level and shape of the mean response profiles of Hsp70 and Hsp101 in I. pumila compared to that expressed at ambient air temperatures. This finding is especially important to forecast the fate of Iris populations under upcoming climate warming.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Variation in Hsp70 and Hsp101 levels in response to experimental warming in Iris pumila L.: an open-topped chamber experiment",
pages = "138",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5047"
}

Hočevar, K., Vuleta, A.,& Manitašević Jovanović, S.. (2022). Variation in Hsp70 and Hsp101 levels in response to experimental warming in Iris pumila L.: an open-topped chamber experiment. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 138.
https://hdl.handle.net/21.15107/rcub_ibiss_5047

Hočevar K, Vuleta A, Manitašević Jovanović S. Variation in Hsp70 and Hsp101 levels in response to experimental warming in Iris pumila L.: an open-topped chamber experiment. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:138.
https://hdl.handle.net/21.15107/rcub_ibiss_5047 .

Hočevar, Katarina, Vuleta, Ana, Manitašević Jovanović, Sanja, "Variation in Hsp70 and Hsp101 levels in response to experimental warming in Iris pumila L.: an open-topped chamber experiment" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):138,
https://hdl.handle.net/21.15107/rcub_ibiss_5047 .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5045
AB  - Plant physiology, fitness and distribution largely depend on temperature, hence it is expected that global warming will extensively alter their biodiversity as well as the ecosystem functioning. Variations in plant functional traits are widely used to predict the impact of global warming on vegetation, as they usually reflect the plant’s adaptive strategies to essential environmental resources, including temperature. To assess how plants respond to climate warming, we investigated the phenotypic expression of two major leaf functional traits, specific leaf area (SLA) and stomatal density (SD), to a temperature rise of 1°C. A total of 40 Iris pumila genotypes, which were consistently cultivated in a growth room at baseline temperature (23/19°C day/night), were exposed to elevated temperature (24/20°C day/night). Within each temperature treatment, the last fully developed leaf was harvested from every genotype and analyzed for the SLA and SD. The mean value of both investigated traits increased significantly with temperature. Leaves developed under elevated temperature exhibited 7% greater SLA and 22% higher SD compared to those developed under ambient temperature. These results suggest that I. pumila plants are able to counteract rising temperatures by the plastic response of SLA and SD. Such a capacity to acclimate its major leaf functional traits to altered temperature conditions may provide I. pumila a resilience to climate warming that will be occurring within its natural habitats.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
T1  - Phenotypic plasticity of Iris pumila leaf functional traits in response to experimental warming
SP  - 576
EP  - 577
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5045
ER  - 

@conference{
author = "Hočevar, Katarina and Vuleta, Ana and Manitašević Jovanović, Sanja",
year = "2022",
abstract = "Plant physiology, fitness and distribution largely depend on temperature, hence it is expected that global warming will extensively alter their biodiversity as well as the ecosystem functioning. Variations in plant functional traits are widely used to predict the impact of global warming on vegetation, as they usually reflect the plant’s adaptive strategies to essential environmental resources, including temperature. To assess how plants respond to climate warming, we investigated the phenotypic expression of two major leaf functional traits, specific leaf area (SLA) and stomatal density (SD), to a temperature rise of 1°C. A total of 40 Iris pumila genotypes, which were consistently cultivated in a growth room at baseline temperature (23/19°C day/night), were exposed to elevated temperature (24/20°C day/night). Within each temperature treatment, the last fully developed leaf was harvested from every genotype and analyzed for the SLA and SD. The mean value of both investigated traits increased significantly with temperature. Leaves developed under elevated temperature exhibited 7% greater SLA and 22% higher SD compared to those developed under ambient temperature. These results suggest that I. pumila plants are able to counteract rising temperatures by the plastic response of SLA and SD. Such a capacity to acclimate its major leaf functional traits to altered temperature conditions may provide I. pumila a resilience to climate warming that will be occurring within its natural habitats.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic",
title = "Phenotypic plasticity of Iris pumila leaf functional traits in response to experimental warming",
pages = "576-577",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5045"
}

Hočevar, K., Vuleta, A.,& Manitašević Jovanović, S.. (2022). Phenotypic plasticity of Iris pumila leaf functional traits in response to experimental warming. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
European Society for Evolutionary Biology., 576-577.
https://hdl.handle.net/21.15107/rcub_ibiss_5045

Hočevar K, Vuleta A, Manitašević Jovanović S. Phenotypic plasticity of Iris pumila leaf functional traits in response to experimental warming. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic. 2022;:576-577.
https://hdl.handle.net/21.15107/rcub_ibiss_5045 .

Hočevar, Katarina, Vuleta, Ana, Manitašević Jovanović, Sanja, "Phenotypic plasticity of Iris pumila leaf functional traits in response to experimental warming" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic (2022):576-577,
https://hdl.handle.net/21.15107/rcub_ibiss_5045 .

TY  - CONF
AU  - Vuleta, Ana
AU  - Hočevar, Katarina
AU  - Manitašević Jovanović, Sanja
AU  - Raičević, Jovana
AU  - Plećaš, Milan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5048
AB  - Nearly all botanists give pollinators credit for the remarkable diversity of flower color, shapes
and sizes observed in nature. However, an increasing number of studies suggests that floral selection is a more pluralistic process, involving not only pollinators but also herbivores and factors
of the plant’s abiotic environment. To estimate Iris pumila pollinator and florivore diversity in a
common garden of Institute for Biological Research in Belgrade, we used a pan trap approach.
Since I. pumila displays a variety of flower color phenotypes ranging from white and yellow to
various shades of purple and blue, we painted the traps in fluorescent purple, blue and yellow. A
total of 391 insects were collected and identifed to family or genus level. In general, insects were
mostly attracted to yellow traps compared to blue and purple ones (69.3%, 19.7% and 11.0%, respectively). The most numerous potential I. pumila pollinators were bees (Anthophila) (89.8%) with
predominantly genus Halictus, while sawflies (Tenthredinidae) were signifcantly less represented
(10.2%) with only genus Athalia. As potential florivores, beetles Tropinota hirta (Scarabaeidae) and
Meligethes sp. (Nitidulidae) were identifed and all individuals were caught in yellow traps, while
specimens of other species from Nitidulidae family were found only in the blue traps. Both pollinators and florivores showed pronounced afnity towards yellow traps, indicating that in the case
of I. pumila insects have potential to be agents of floral selection. Nevertheless, it is necessary to
evaluate pollinator and florivore diversity and their color preferences in I. pumila natural habitat.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Assessment of Iris pumila L. pollinator and florivore diversity in a common garden: a pan-trap experiment
SP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5048
ER  - 

@conference{
author = "Vuleta, Ana and Hočevar, Katarina and Manitašević Jovanović, Sanja and Raičević, Jovana and Plećaš, Milan",
year = "2022",
abstract = "Nearly all botanists give pollinators credit for the remarkable diversity of flower color, shapes
and sizes observed in nature. However, an increasing number of studies suggests that floral selection is a more pluralistic process, involving not only pollinators but also herbivores and factors
of the plant’s abiotic environment. To estimate Iris pumila pollinator and florivore diversity in a
common garden of Institute for Biological Research in Belgrade, we used a pan trap approach.
Since I. pumila displays a variety of flower color phenotypes ranging from white and yellow to
various shades of purple and blue, we painted the traps in fluorescent purple, blue and yellow. A
total of 391 insects were collected and identifed to family or genus level. In general, insects were
mostly attracted to yellow traps compared to blue and purple ones (69.3%, 19.7% and 11.0%, respectively). The most numerous potential I. pumila pollinators were bees (Anthophila) (89.8%) with
predominantly genus Halictus, while sawflies (Tenthredinidae) were signifcantly less represented
(10.2%) with only genus Athalia. As potential florivores, beetles Tropinota hirta (Scarabaeidae) and
Meligethes sp. (Nitidulidae) were identifed and all individuals were caught in yellow traps, while
specimens of other species from Nitidulidae family were found only in the blue traps. Both pollinators and florivores showed pronounced afnity towards yellow traps, indicating that in the case
of I. pumila insects have potential to be agents of floral selection. Nevertheless, it is necessary to
evaluate pollinator and florivore diversity and their color preferences in I. pumila natural habitat.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Assessment of Iris pumila L. pollinator and florivore diversity in a common garden: a pan-trap experiment",
pages = "142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5048"
}

Vuleta, A., Hočevar, K., Manitašević Jovanović, S., Raičević, J.,& Plećaš, M.. (2022). Assessment of Iris pumila L. pollinator and florivore diversity in a common garden: a pan-trap experiment. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 142.
https://hdl.handle.net/21.15107/rcub_ibiss_5048

Vuleta A, Hočevar K, Manitašević Jovanović S, Raičević J, Plećaš M. Assessment of Iris pumila L. pollinator and florivore diversity in a common garden: a pan-trap experiment. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:142.
https://hdl.handle.net/21.15107/rcub_ibiss_5048 .

Vuleta, Ana, Hočevar, Katarina, Manitašević Jovanović, Sanja, Raičević, Jovana, Plećaš, Milan, "Assessment of Iris pumila L. pollinator and florivore diversity in a common garden: a pan-trap experiment" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):142,
https://hdl.handle.net/21.15107/rcub_ibiss_5048 .

TY  - CONF
AU  - Manitašević Jovanović, Sanja
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5055
AB  - The ongoing climate change creates critical challenges for plant population persistence in
natural communities. To investigate plant responses to future climate warming conditions, we
employed an in situ temperature manipulation experiment in two sun-exposed natural populations of Iris pumila. One half of each randomly chosen clonal plant was experimentally warmed
(about 1-2 ºC) using a clear-sided open top chamber (OTC), while the other half faced the ambient temperature conditions. During the summer season, phenotypic values of key functional
leaf traits - leaf dry mass per area (LMA), leaf succulence (SU) and stomatal density (SD) - were
recorded simultaneously on the ramets from each clone growing inside and outside of the OTC.
The phenotypic expression of all analyzed traits changed with temperature, being smaller in the
ramets developing within the OTCs compared to those growing under ambient temperature conditions. Observed difference was found to be statistically signifcant for LMA and SU, while those
related to SD could be described as tendency. In addition, the association between the pairs of
leaf and abiotic variables was tested. The recorded soil temperature appeared to be signifcantly
concordant with SD, but, as well as leaf and air temperature, discordant with SU. Our results suggest that I. pumila plants have the capacity to adjust to rising temperatures by plastic responses of
leaf functional traits. This is of great importance to anticipate the future of Iris populations under
upcoming warmer climate.
PB  - Belgrade: Serbian Biological Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Plastic responses of Iris pumila leaf functional traits to experimental warming in situ
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5055
ER  - 

@conference{
author = "Manitašević Jovanović, Sanja and Hočevar, Katarina and Vuleta, Ana",
year = "2022",
abstract = "The ongoing climate change creates critical challenges for plant population persistence in
natural communities. To investigate plant responses to future climate warming conditions, we
employed an in situ temperature manipulation experiment in two sun-exposed natural populations of Iris pumila. One half of each randomly chosen clonal plant was experimentally warmed
(about 1-2 ºC) using a clear-sided open top chamber (OTC), while the other half faced the ambient temperature conditions. During the summer season, phenotypic values of key functional
leaf traits - leaf dry mass per area (LMA), leaf succulence (SU) and stomatal density (SD) - were
recorded simultaneously on the ramets from each clone growing inside and outside of the OTC.
The phenotypic expression of all analyzed traits changed with temperature, being smaller in the
ramets developing within the OTCs compared to those growing under ambient temperature conditions. Observed difference was found to be statistically signifcant for LMA and SU, while those
related to SD could be described as tendency. In addition, the association between the pairs of
leaf and abiotic variables was tested. The recorded soil temperature appeared to be signifcantly
concordant with SD, but, as well as leaf and air temperature, discordant with SU. Our results suggest that I. pumila plants have the capacity to adjust to rising temperatures by plastic responses of
leaf functional traits. This is of great importance to anticipate the future of Iris populations under
upcoming warmer climate.",
publisher = "Belgrade: Serbian Biological Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Plastic responses of Iris pumila leaf functional traits to experimental warming in situ",
pages = "143",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5055"
}

Manitašević Jovanović, S., Hočevar, K.,& Vuleta, A.. (2022). Plastic responses of Iris pumila leaf functional traits to experimental warming in situ. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Biological Society., 143.
https://hdl.handle.net/21.15107/rcub_ibiss_5055

Manitašević Jovanović S, Hočevar K, Vuleta A. Plastic responses of Iris pumila leaf functional traits to experimental warming in situ. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:143.
https://hdl.handle.net/21.15107/rcub_ibiss_5055 .

Manitašević Jovanović, Sanja, Hočevar, Katarina, Vuleta, Ana, "Plastic responses of Iris pumila leaf functional traits to experimental warming in situ" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):143,
https://hdl.handle.net/21.15107/rcub_ibiss_5055 .

TY  - CONF
AU  - Manitašević Jovanović, Sanja
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5056
AB  - The ongoing climate change creates critical challenges for plant population persistence innatural communities. To investigate plant responses to future climate warming conditions, weemployed an in situ temperature manipulation experiment in two sun-exposed natural populations of Iris pumila. One half of each randomly chosen clonal plant was experimentally warmed(about 1-2 ºC) using a clear-sided open top chamber (OTC), while the other half faced the ambient temperature conditions. During the summer season, phenotypic values of key functionalleaf traits - leaf dry mass per area (LMA), leaf succulence (SU) and stomatal density (SD) - wererecorded simultaneously on the ramets from each clone growing inside and outside of the OTC.The phenotypic expression of all analyzed traits changed with temperature, being smaller in theramets developing within the OTCs compared to those growing under ambient temperature conditions. Observed difference was found to be statistically signifcant for LMA and SU, while thoserelated to SD could be described as tendency. In addition, the association between the pairs ofleaf and abiotic variables was tested. The recorded soil temperature appeared to be signifcantlyconcordant with SD, but, as well as leaf and air temperature, discordant with SU. Our results suggest that I. pumila plants have the capacity to adjust to rising temperatures by plastic responses ofleaf functional traits. This is of great importance to anticipate the future of Iris populations underupcoming warmer climate.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Plastic responses of Iris pumila leaf functional traits to experimental warming in situ
SP  - PP4-5
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5056
ER  - 

@conference{
author = "Manitašević Jovanović, Sanja and Hočevar, Katarina and Vuleta, Ana",
year = "2022",
abstract = "The ongoing climate change creates critical challenges for plant population persistence innatural communities. To investigate plant responses to future climate warming conditions, weemployed an in situ temperature manipulation experiment in two sun-exposed natural populations of Iris pumila. One half of each randomly chosen clonal plant was experimentally warmed(about 1-2 ºC) using a clear-sided open top chamber (OTC), while the other half faced the ambient temperature conditions. During the summer season, phenotypic values of key functionalleaf traits - leaf dry mass per area (LMA), leaf succulence (SU) and stomatal density (SD) - wererecorded simultaneously on the ramets from each clone growing inside and outside of the OTC.The phenotypic expression of all analyzed traits changed with temperature, being smaller in theramets developing within the OTCs compared to those growing under ambient temperature conditions. Observed difference was found to be statistically signifcant for LMA and SU, while thoserelated to SD could be described as tendency. In addition, the association between the pairs ofleaf and abiotic variables was tested. The recorded soil temperature appeared to be signifcantlyconcordant with SD, but, as well as leaf and air temperature, discordant with SU. Our results suggest that I. pumila plants have the capacity to adjust to rising temperatures by plastic responses ofleaf functional traits. This is of great importance to anticipate the future of Iris populations underupcoming warmer climate.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Plastic responses of Iris pumila leaf functional traits to experimental warming in situ",
pages = "PP4-5",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5056"
}

Manitašević Jovanović, S., Hočevar, K.,& Vuleta, A.. (2022). Plastic responses of Iris pumila leaf functional traits to experimental warming in situ. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade., PP4-5.
https://hdl.handle.net/21.15107/rcub_ibiss_5056

Manitašević Jovanović S, Hočevar K, Vuleta A. Plastic responses of Iris pumila leaf functional traits to experimental warming in situ. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:PP4-5.
https://hdl.handle.net/21.15107/rcub_ibiss_5056 .

Manitašević Jovanović, Sanja, Hočevar, Katarina, Vuleta, Ana, "Plastic responses of Iris pumila leaf functional traits to experimental warming in situ" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):PP4-5,
https://hdl.handle.net/21.15107/rcub_ibiss_5056 .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5046
AB  - Plant physiology, fitness and distribution largely depend on temperature, hence it is expected that global warming will extensively alter their biodiversity as well as the ecosystem functioning. Variations in plant functional traits are widely used to predict the impact of global warming on vegetation, as they usually reflect the plant’s adaptive strategies to essential environmental resources, including temperature. To assess how plants respond to climate warming, we investigated the phenotypic expression of two major leaf functional traits, specific leaf area (SLA) and stomatal density (SD), to a temperature rise of 1°C. A total of 40 Iris pumila genotypes, which were consistently cultivated in a growth room at baseline temperature (23/19°C day/night), were exposed to elevated temperature (24/20°C day/night). Within each temperature treatment, the last fully developed leaf was harvested from every genotype and analyzed for the SLA and SD. The mean value of both investigated traits increased significantly with temperature. Leaves developed under elevated temperature exhibited 7% greater SLA and 22% higher SD compared to those developed under ambient temperature. These results suggest that I. pumila plants are able to counteract rising temperatures by the plastic response of SLA and SD. Such a capacity to acclimate its major leaf functional traits to altered temperature conditions may provide I. pumila a resilience to climate warming that will be occurring within its natural habitats.
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Phenotypic plasticity of Iris pumila  leaf functional traits in response to experimental warming
SP  - P348
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5046
ER  - 

@conference{
author = "Hočevar, Katarina and Vuleta, Ana and Manitašević Jovanović, Sanja",
year = "2022",
abstract = "Plant physiology, fitness and distribution largely depend on temperature, hence it is expected that global warming will extensively alter their biodiversity as well as the ecosystem functioning. Variations in plant functional traits are widely used to predict the impact of global warming on vegetation, as they usually reflect the plant’s adaptive strategies to essential environmental resources, including temperature. To assess how plants respond to climate warming, we investigated the phenotypic expression of two major leaf functional traits, specific leaf area (SLA) and stomatal density (SD), to a temperature rise of 1°C. A total of 40 Iris pumila genotypes, which were consistently cultivated in a growth room at baseline temperature (23/19°C day/night), were exposed to elevated temperature (24/20°C day/night). Within each temperature treatment, the last fully developed leaf was harvested from every genotype and analyzed for the SLA and SD. The mean value of both investigated traits increased significantly with temperature. Leaves developed under elevated temperature exhibited 7% greater SLA and 22% higher SD compared to those developed under ambient temperature. These results suggest that I. pumila plants are able to counteract rising temperatures by the plastic response of SLA and SD. Such a capacity to acclimate its major leaf functional traits to altered temperature conditions may provide I. pumila a resilience to climate warming that will be occurring within its natural habitats.",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Phenotypic plasticity of Iris pumila  leaf functional traits in response to experimental warming",
pages = "P348",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5046"
}

Hočevar, K., Vuleta, A.,& Manitašević Jovanović, S.. (2022). Phenotypic plasticity of Iris pumila  leaf functional traits in response to experimental warming. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic, P348.
https://hdl.handle.net/21.15107/rcub_ibiss_5046

Hočevar K, Vuleta A, Manitašević Jovanović S. Phenotypic plasticity of Iris pumila  leaf functional traits in response to experimental warming. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:P348.
https://hdl.handle.net/21.15107/rcub_ibiss_5046 .

Hočevar, Katarina, Vuleta, Ana, Manitašević Jovanović, Sanja, "Phenotypic plasticity of Iris pumila  leaf functional traits in response to experimental warming" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):P348,
https://hdl.handle.net/21.15107/rcub_ibiss_5046 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Kostić, Miroslav
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Šešlija Jovanović, Darka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3837
AB  - The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Insects
T1  - Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus
IS  - 9
VL  - 11
DO  - 10.3390/insects11090563
SP  - 563
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Kostić, Miroslav and Vuleta, Ana and Manitašević Jovanović, Sanja and Šešlija Jovanović, Darka",
year = "2020",
abstract = "The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Insects",
title = "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus",
number = "9",
volume = "11",
doi = "10.3390/insects11090563",
pages = "563"
}

Lazarević, J., Jevremović, S., Kostić, I., Kostić, M., Vuleta, A., Manitašević Jovanović, S.,& Šešlija Jovanović, D.. (2020). Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects
Multidisciplinary Digital Publishing Institute., 11(9), 563.
https://doi.org/10.3390/insects11090563

Lazarević J, Jevremović S, Kostić I, Kostić M, Vuleta A, Manitašević Jovanović S, Šešlija Jovanović D. Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects. 2020;11(9):563.
doi:10.3390/insects11090563 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Kostić, Miroslav, Vuleta, Ana, Manitašević Jovanović, Sanja, Šešlija Jovanović, Darka, "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus" in Insects, 11, no. 9 (2020):563,
https://doi.org/10.3390/insects11090563 . .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
PY  - 2019
UR  - https://app.oxfordabstracts.com/events/653/program-app/submission/123447
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3546
AB  - To predict short-term plant responses to global warming, an open-topped-chamber experiment was conducted in two sun-exposed natural populations of Iris pumila during a growing season. In each population, one half of all randomly selected clonal plants were experimentally warmed using a clear-sided, open-topped chamber (OTC), while the other half experienced the ambient temperature conditions. The air temperature within the OTCs was approx.1-2 ºC higher than that outside of the OTCs in general. In spring and summer of 2018, phenotypic values of the three functional leaf traits: specific leaf area (SLA), leaf dry mass content (LDMC), and leaf water content (LWC) were recorded on the ramets from each clone growing inside and outside of the OTC. The phenotypic expressions of all analyzed traits changed significantly with temperature in both populations, but in an opposite direction. While SLA and LWC tended to be greater in the ramets developing within the OTCs relative to those growing outside of them, their LDMC values reversed, being higher inside than outside of the OTCs. The analyzed traits varied seasonally as well. Regardless of the location within a clone, the values of SLA and LWC were greater in spring then in summer. Conversely, LDMC reached its maximum in the warmer season. Our results clearly suggest that I. pumila plants have the capacity to cope with increasing temperatures by plastic responses of leaf functional traits. This finding is of crucial importance to forecast the fate of Iris populations under global warming - that is, whether they will adopt or disappear.
PB  - The European Society for Evolutionary Biology
C3  - 17th Congress of the European Society for Evolutionary Biology
T1  - Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment
SP  - S36e.P12
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3546
ER  - 

@conference{
author = "Hočevar, Katarina and Budečević, Sanja and Vuleta, Ana and Manitašević Jovanović, Sanja",
year = "2019",
abstract = "To predict short-term plant responses to global warming, an open-topped-chamber experiment was conducted in two sun-exposed natural populations of Iris pumila during a growing season. In each population, one half of all randomly selected clonal plants were experimentally warmed using a clear-sided, open-topped chamber (OTC), while the other half experienced the ambient temperature conditions. The air temperature within the OTCs was approx.1-2 ºC higher than that outside of the OTCs in general. In spring and summer of 2018, phenotypic values of the three functional leaf traits: specific leaf area (SLA), leaf dry mass content (LDMC), and leaf water content (LWC) were recorded on the ramets from each clone growing inside and outside of the OTC. The phenotypic expressions of all analyzed traits changed significantly with temperature in both populations, but in an opposite direction. While SLA and LWC tended to be greater in the ramets developing within the OTCs relative to those growing outside of them, their LDMC values reversed, being higher inside than outside of the OTCs. The analyzed traits varied seasonally as well. Regardless of the location within a clone, the values of SLA and LWC were greater in spring then in summer. Conversely, LDMC reached its maximum in the warmer season. Our results clearly suggest that I. pumila plants have the capacity to cope with increasing temperatures by plastic responses of leaf functional traits. This finding is of crucial importance to forecast the fate of Iris populations under global warming - that is, whether they will adopt or disappear.",
publisher = "The European Society for Evolutionary Biology",
journal = "17th Congress of the European Society for Evolutionary Biology",
title = "Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment",
pages = "S36e.P12",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3546"
}

Hočevar, K., Budečević, S., Vuleta, A.,& Manitašević Jovanović, S.. (2019). Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment. in 17th Congress of the European Society for Evolutionary Biology
The European Society for Evolutionary Biology., S36e.P12.
https://hdl.handle.net/21.15107/rcub_ibiss_3546

Hočevar K, Budečević S, Vuleta A, Manitašević Jovanović S. Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment. in 17th Congress of the European Society for Evolutionary Biology. 2019;:S36e.P12.
https://hdl.handle.net/21.15107/rcub_ibiss_3546 .

Hočevar, Katarina, Budečević, Sanja, Vuleta, Ana, Manitašević Jovanović, Sanja, "Predicting ecological responses to global warming in Iris pumila: an open-topped chamber experiment" in 17th Congress of the European Society for Evolutionary Biology (2019):S36e.P12,
https://hdl.handle.net/21.15107/rcub_ibiss_3546 .

TY  - CONF
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Hočevar, Katarina
AU  - Tucić, Branka
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5061
AB  - Flowers are complex phenotypic structures composed of four functionally distinct organs arranged in concentric floral whorls: sepals in the frst, petals in the second, and two inner whorls
consisting of reproductive structures: stamens in the third whorl and tricarpellary gynoecium, in
the centre. Color, size and shape of these floral organs are considered to be important signals that
can attract the pollinators, advertising the plants’ floral reward (commonly nectar and pollen).
Here we use the methods of geometric morphometrics to compare size and shape variation of
three functionally different floral organs: falls, standards and style arms, among fve distinct color
morphs (blue, violet, dark blue, dark violet and yellow). Pairwise comparisons of the centroid size
means showed that all floral organs differed signifcantly among the analysed color phenotypes.
The mean shape of falls, standards and style arms appeared to be flower color-specifc as well, but
most noticeably between yellow - on one side, and blue/violet morphs - on the other. Procrustes
ANOVAs revealed the signifcant presence of directional and fluctuating asymmetry for falls and
standards among all fve color morphs, with the exception of style arms. The observed morphological diversifcation of floral organs among distinctive I. pumila color phenotypes could be the
adaptive outcome of the interactions with their pollinators.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5061
ER  - 

@conference{
author = "Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Hočevar, Katarina and Tucić, Branka",
year = "2018",
abstract = "Flowers are complex phenotypic structures composed of four functionally distinct organs arranged in concentric floral whorls: sepals in the frst, petals in the second, and two inner whorls
consisting of reproductive structures: stamens in the third whorl and tricarpellary gynoecium, in
the centre. Color, size and shape of these floral organs are considered to be important signals that
can attract the pollinators, advertising the plants’ floral reward (commonly nectar and pollen).
Here we use the methods of geometric morphometrics to compare size and shape variation of
three functionally different floral organs: falls, standards and style arms, among fve distinct color
morphs (blue, violet, dark blue, dark violet and yellow). Pairwise comparisons of the centroid size
means showed that all floral organs differed signifcantly among the analysed color phenotypes.
The mean shape of falls, standards and style arms appeared to be flower color-specifc as well, but
most noticeably between yellow - on one side, and blue/violet morphs - on the other. Procrustes
ANOVAs revealed the signifcant presence of directional and fluctuating asymmetry for falls and
standards among all fve color morphs, with the exception of style arms. The observed morphological diversifcation of floral organs among distinctive I. pumila color phenotypes could be the
adaptive outcome of the interactions with their pollinators.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?",
pages = "82",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5061"
}

Budečević, S., Manitašević Jovanović, S., Vuleta, A., Hočevar, K.,& Tucić, B.. (2018). Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 82.
https://hdl.handle.net/21.15107/rcub_ibiss_5061

Budečević S, Manitašević Jovanović S, Vuleta A, Hočevar K, Tucić B. Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:82.
https://hdl.handle.net/21.15107/rcub_ibiss_5061 .

Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Hočevar, Katarina, Tucić, Branka, "Morphological diversity of functionally distinctive floral organs in Iris pumila: Does the flower color matter?" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):82,
https://hdl.handle.net/21.15107/rcub_ibiss_5061 .

TY  - CONF
AU  - Vuleta, Ana
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5062
AB  - Angiosperm flowers serve as an advertisement for pollinators and are, therefore, believed to
be shaped by pollinator-mediated selection. The large flower size, which is one of the distinct
characteristics of the genus Iris, might have evolved under the strong selective pressures imposed
by pollinators, either because larger flowers indicate more rewards or because the pollinators can
detect them from a greater distance. To test the role of visual floral signals in attracting pollinators and, consequently, pollination efciency, we compared the phenotypic expressions of flower
height and centroid size of petaloid floral organs: falls, standards and style arms, as well as anthocyanin absorption between naturally pollinated and non-pollinated flowers of I. pumila plants
grown in a common-garden experiment. Our results indicated that I. pumila pollinators generally
preferred taller flowers with greater organ sizes compared to the alternative ones. However, the
direction of pollinator-mediated selection appeared to be strongly flower organ-specifc: positive
on fall, negative on style arm and neutral on standard size. The observed results are in agreement
with the functions that each of these floral structures has in the pollination process: standards are
a long-distance reward signals, falls are landing platform for pollinating insects, while style arms,
as upper parts of the pollination tunnels, promote pollen deposition. We failed to corroborate the
existence of pollinator-mediated selection on anthocyanin absorption. This suggested that some
other aspects of flower colour might be more attractive to insect pollinators, or that different biotic or abiotic factors could account for the maintenance of flower colour polymorphism in I. pumila.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Do pollinators prefer bigger floral organs? A case study on Iris pumila L.
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5062
ER  - 

@conference{
author = "Vuleta, Ana and Budečević, Sanja and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2018",
abstract = "Angiosperm flowers serve as an advertisement for pollinators and are, therefore, believed to
be shaped by pollinator-mediated selection. The large flower size, which is one of the distinct
characteristics of the genus Iris, might have evolved under the strong selective pressures imposed
by pollinators, either because larger flowers indicate more rewards or because the pollinators can
detect them from a greater distance. To test the role of visual floral signals in attracting pollinators and, consequently, pollination efciency, we compared the phenotypic expressions of flower
height and centroid size of petaloid floral organs: falls, standards and style arms, as well as anthocyanin absorption between naturally pollinated and non-pollinated flowers of I. pumila plants
grown in a common-garden experiment. Our results indicated that I. pumila pollinators generally
preferred taller flowers with greater organ sizes compared to the alternative ones. However, the
direction of pollinator-mediated selection appeared to be strongly flower organ-specifc: positive
on fall, negative on style arm and neutral on standard size. The observed results are in agreement
with the functions that each of these floral structures has in the pollination process: standards are
a long-distance reward signals, falls are landing platform for pollinating insects, while style arms,
as upper parts of the pollination tunnels, promote pollen deposition. We failed to corroborate the
existence of pollinator-mediated selection on anthocyanin absorption. This suggested that some
other aspects of flower colour might be more attractive to insect pollinators, or that different biotic or abiotic factors could account for the maintenance of flower colour polymorphism in I. pumila.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Do pollinators prefer bigger floral organs? A case study on Iris pumila L.",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5062"
}

Vuleta, A., Budečević, S., Manitašević Jovanović, S.,& Tucić, B.. (2018). Do pollinators prefer bigger floral organs? A case study on Iris pumila L.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 86.
https://hdl.handle.net/21.15107/rcub_ibiss_5062

Vuleta A, Budečević S, Manitašević Jovanović S, Tucić B. Do pollinators prefer bigger floral organs? A case study on Iris pumila L.. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:86.
https://hdl.handle.net/21.15107/rcub_ibiss_5062 .

Vuleta, Ana, Budečević, Sanja, Manitašević Jovanović, Sanja, Tucić, Branka, "Do pollinators prefer bigger floral organs? A case study on Iris pumila L." in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):86,
https://hdl.handle.net/21.15107/rcub_ibiss_5062 .

TY  - JOUR
AU  - Tucić, Branka
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
AU  - Vuleta, Ana
AU  - Klingenberg, Christian Peter
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jeb.13207
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29134739
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2927
AB  - Fluctuating asymmetry (FA) is widely used to quantify developmental instability (DI) in ecological and evolutionary studies. It has long been recognized that FA may not exclusively originate from DI for sessile organisms such as plants, because phenotypic plasticity in response to heterogeneities in the environment might also produce FA. This study provides the first empirical evidence for this hypothesis. We reasoned that solar irradiance, which is greater on the southern side than on the northern side of plants growing in the temperate zone of the Northern Hemisphere, would cause systematic morphological differences and asymmetry associated with the orientation of plant parts. We used geometric morphometrics to characterize the size and shape of flower parts in Iris pumila grown in a common garden. The size of floral organs was not significantly affected by orientation. Shape and particularly its asymmetric component differed significantly according to orientation for three different floral parts. Orientation accounted for 10.4% of the total shape asymmetry within flowers in the falls, for 11.4% in the standards and for 2.2% in the style branches. This indicates that phenotypic plasticity in response to a directed environmental factor, most likely solar irradiance, contributes to FA of flowers under natural conditions. That FA partly results from phenotypic plasticity and not just from DI needs to be considered by studies of FA in plants and other sessile organisms.
T2  - Journal of Evolutionary Biology
T1  - Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.
IS  - 2
VL  - 31
DO  - 10.1111/jeb.13207
SP  - 197
EP  - 210
ER  - 

@article{
author = "Tucić, Branka and Budečević, Sanja and Manitašević Jovanović, Sanja and Vuleta, Ana and Klingenberg, Christian Peter",
year = "2018",
abstract = "Fluctuating asymmetry (FA) is widely used to quantify developmental instability (DI) in ecological and evolutionary studies. It has long been recognized that FA may not exclusively originate from DI for sessile organisms such as plants, because phenotypic plasticity in response to heterogeneities in the environment might also produce FA. This study provides the first empirical evidence for this hypothesis. We reasoned that solar irradiance, which is greater on the southern side than on the northern side of plants growing in the temperate zone of the Northern Hemisphere, would cause systematic morphological differences and asymmetry associated with the orientation of plant parts. We used geometric morphometrics to characterize the size and shape of flower parts in Iris pumila grown in a common garden. The size of floral organs was not significantly affected by orientation. Shape and particularly its asymmetric component differed significantly according to orientation for three different floral parts. Orientation accounted for 10.4% of the total shape asymmetry within flowers in the falls, for 11.4% in the standards and for 2.2% in the style branches. This indicates that phenotypic plasticity in response to a directed environmental factor, most likely solar irradiance, contributes to FA of flowers under natural conditions. That FA partly results from phenotypic plasticity and not just from DI needs to be considered by studies of FA in plants and other sessile organisms.",
journal = "Journal of Evolutionary Biology",
title = "Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.",
number = "2",
volume = "31",
doi = "10.1111/jeb.13207",
pages = "197-210"
}

Tucić, B., Budečević, S., Manitašević Jovanović, S., Vuleta, A.,& Klingenberg, C. P.. (2018). Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.. in Journal of Evolutionary Biology, 31(2), 197-210.
https://doi.org/10.1111/jeb.13207

Tucić B, Budečević S, Manitašević Jovanović S, Vuleta A, Klingenberg CP. Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence.. in Journal of Evolutionary Biology. 2018;31(2):197-210.
doi:10.1111/jeb.13207 .

Tucić, Branka, Budečević, Sanja, Manitašević Jovanović, Sanja, Vuleta, Ana, Klingenberg, Christian Peter, "Phenotypic plasticity in response to environmental heterogeneity contributes to fluctuating asymmetry in plants: first empirical evidence." in Journal of Evolutionary Biology, 31, no. 2 (2018):197-210,
https://doi.org/10.1111/jeb.13207 . .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5059
AB  - The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that play
essential roles in numerous cellular processes, including the folding of newly synthesized and
stress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s in
different floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty seven
genotypes raised in a common-garden and belonging to three colour classes: yellow, dark blue
and dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70
was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoform
was expressed in the standards and falls, the stamens and style arms expressed two isoforms. The
relative abundance of Hsp70 varied among differed flower organs as well. The lowest value was
recorded in the standards and the highest in the stamens, whereas its abundance in the falls and
style arms appeared to be intermediate. Such variation pattern was detected exclusively in the
dark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidence
for organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila
SP  - 89
EP  - 90
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5059
ER  - 

@conference{
author = "Hočevar, Katarina and Budečević, Sanja and Manitašević Jovanović, Sanja",
year = "2018",
abstract = "The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that play
essential roles in numerous cellular processes, including the folding of newly synthesized and
stress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s in
different floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty seven
genotypes raised in a common-garden and belonging to three colour classes: yellow, dark blue
and dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70
was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoform
was expressed in the standards and falls, the stamens and style arms expressed two isoforms. The
relative abundance of Hsp70 varied among differed flower organs as well. The lowest value was
recorded in the standards and the highest in the stamens, whereas its abundance in the falls and
style arms appeared to be intermediate. Such variation pattern was detected exclusively in the
dark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidence
for organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila",
pages = "89-90",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5059"
}

Hočevar, K., Budečević, S.,& Manitašević Jovanović, S.. (2018). Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 89-90.
https://hdl.handle.net/21.15107/rcub_ibiss_5059

Hočevar K, Budečević S, Manitašević Jovanović S. Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:89-90.
https://hdl.handle.net/21.15107/rcub_ibiss_5059 .

Hočevar, Katarina, Budečević, Sanja, Manitašević Jovanović, Sanja, "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):89-90,
https://hdl.handle.net/21.15107/rcub_ibiss_5059 .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5060
AB  - The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that playessential roles in numerous cellular processes, including the folding of newly synthesized andstress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s indifferent floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty sevengenotypes raised in a common-garden and belonging to three colour classes: yellow, dark blueand dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoformwas expressed in the standards and falls, the stamens and style arms expressed two isoforms. Therelative abundance of Hsp70 varied among differed flower organs as well. The lowest value wasrecorded in the standards and the highest in the stamens, whereas its abundance in the falls andstyle arms appeared to be intermediate. Such variation pattern was detected exclusively in thedark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidencefor organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila
SP  - PP3-8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5060
ER  - 

@conference{
author = "Hočevar, Katarina and Budečević, Sanja and Manitašević Jovanović, Sanja",
year = "2018",
abstract = "The 70kD heat shock proteins (Hsp70s) are highly conserved molecular chaperones that playessential roles in numerous cellular processes, including the folding of newly synthesized andstress-denatured proteins, the translocation of proteins across membranes, as well as the degradation of aggregated proteins. Here we examined the variation in the abundance of Hsp70s indifferent floral organs of the three distinctive color morphs in Iris pumila. Flowers of twenty sevengenotypes raised in a common-garden and belonging to three colour classes: yellow, dark blueand dark violet, were collected (9 per each class) for an immunoblot analysis. The content of Hsp70was quantifed in four floral organs: falls, standards, stamens and style arms. The immunoblot analysis revealed that the expression of Hsp70s was floral organ-specifc. While only one Hsp70 isoformwas expressed in the standards and falls, the stamens and style arms expressed two isoforms. Therelative abundance of Hsp70 varied among differed flower organs as well. The lowest value wasrecorded in the standards and the highest in the stamens, whereas its abundance in the falls andstyle arms appeared to be intermediate. Such variation pattern was detected exclusively in thedark violet colour morphs. In the yellow flowers, however, the amount of Hsp70 tended to be lower compared to the dark blue or dark violet colour morphs. This study provides original evidencefor organ-specifc Hsp70 expression in flowers of I. pumila plants, likely due to the differential metabolic activities occurring in the cells of these functionally distinctive floral structures.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila",
pages = "PP3-8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5060"
}

Hočevar, K., Budečević, S.,& Manitašević Jovanović, S.. (2018). Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade., PP3-8.
https://hdl.handle.net/21.15107/rcub_ibiss_5060

Hočevar K, Budečević S, Manitašević Jovanović S. Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:PP3-8.
https://hdl.handle.net/21.15107/rcub_ibiss_5060 .

Hočevar, Katarina, Budečević, Sanja, Manitašević Jovanović, Sanja, "Variation in the amount of Hsp70 among different floral organs in three alternative color phenotypes of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):PP3-8,
https://hdl.handle.net/21.15107/rcub_ibiss_5060 .

TY  - CONF
AU  - Manitašević Jovanović, Sanja
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5057
AB  - Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higher
plants. Beside their impacts on flower color, they are involved in plant growth, development and
protection against a wide range of environmental stressors. They act either as light attenuators
and free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching
(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,
ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes
(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype was
harvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,
flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens and
style arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern of
the analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appeared
to be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blue
and dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.
Conversely, the anthocyanin content was rather high in the floral organs of dark violet and dark
blue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that a
greater accumulation of both plant pigments in sterile rather than in fertile flower organs could be
causally related to their specifc function in the process of sexual reproduction, where the former
serve to attract plant pollinators, while the latter promote an efcient pollen deposition.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Flavonoid and carotenoid content in floral organs of Iris pumila
SP  - 93
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5057
ER  - 

@conference{
author = "Manitašević Jovanović, Sanja and Hočevar, Katarina and Budečević, Sanja",
year = "2018",
abstract = "Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higher
plants. Beside their impacts on flower color, they are involved in plant growth, development and
protection against a wide range of environmental stressors. They act either as light attenuators
and free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching
(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,
ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes
(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype was
harvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,
flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens and
style arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern of
the analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appeared
to be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blue
and dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.
Conversely, the anthocyanin content was rather high in the floral organs of dark violet and dark
blue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that a
greater accumulation of both plant pigments in sterile rather than in fertile flower organs could be
causally related to their specifc function in the process of sexual reproduction, where the former
serve to attract plant pollinators, while the latter promote an efcient pollen deposition.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Flavonoid and carotenoid content in floral organs of Iris pumila",
pages = "93-94",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5057"
}

Manitašević Jovanović, S., Hočevar, K.,& Budečević, S.. (2018). Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 93-94.
https://hdl.handle.net/21.15107/rcub_ibiss_5057

Manitašević Jovanović S, Hočevar K, Budečević S. Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:93-94.
https://hdl.handle.net/21.15107/rcub_ibiss_5057 .

Manitašević Jovanović, Sanja, Hočevar, Katarina, Budečević, Sanja, "Flavonoid and carotenoid content in floral organs of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):93-94,
https://hdl.handle.net/21.15107/rcub_ibiss_5057 .

TY  - CONF
AU  - Manitašević Jovanović, Sanja
AU  - Hočevar, Katarina
AU  - Budečević, Sanja
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5058
AB  - Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higherplants. Beside their impacts on flower color, they are involved in plant growth, development andprotection against a wide range of environmental stressors. They act either as light attenuatorsand free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype washarvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens andstyle arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern ofthe analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appearedto be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blueand dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.Conversely, the anthocyanin content was rather high in the floral organs of dark violet and darkblue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that agreater accumulation of both plant pigments in sterile rather than in fertile flower organs could becausally related to their specifc function in the process of sexual reproduction, where the formerserve to attract plant pollinators, while the latter promote an efcient pollen deposition.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Flavonoid and carotenoid content in floral organs of Iris pumila
SP  - PP3-14
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5058
ER  - 

@conference{
author = "Manitašević Jovanović, Sanja and Hočevar, Katarina and Budečević, Sanja",
year = "2018",
abstract = "Flavonoids and carotenoids are plant pigments that play a variety of functional roles in higherplants. Beside their impacts on flower color, they are involved in plant growth, development andprotection against a wide range of environmental stressors. They act either as light attenuatorsand free radical scavengers (e.g. anthocyanins) or participate in non-photochemical quenching(carotenoids). To quantify the accumulation of flavonoids and carotenoids in different floral organs,ffty Iris pumila genotypes grown in a common-garden and belonging to different color classes(yellow, blue, violet, dark blue and dark violet) were assessed. One flower per each genotype washarvested and analyzed spectrophotometrically for the content of three flavonoids (anthocyanins,flavones, flavonols) and total carotenoids in different floral organs: falls, standards, stamens andstyle arms. While the quantity of flavonoids was found to be floral organ- and color morph-specific, the amount of carotenoids varied only among individual floral organs. The variation pattern ofthe analyzed compounds displayed identical trend - the lowest amounts were recorded in the stamen and style arm, and the highest in the standard and fall. The content of carotenoids appearedto be the highest in floral organs of the yellow color morphs, whereas in the blue, violet, dark blueand dark violet flower phenotypes its abundance was a half of that recorded for the yellow one.Conversely, the anthocyanin content was rather high in the floral organs of dark violet and darkblue color morphs, but extremely low in those of the yellow one. Our results clearly suggest that agreater accumulation of both plant pigments in sterile rather than in fertile flower organs could becausally related to their specifc function in the process of sexual reproduction, where the formerserve to attract plant pollinators, while the latter promote an efcient pollen deposition.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Flavonoid and carotenoid content in floral organs of Iris pumila",
pages = "PP3-14",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5058"
}

Manitašević Jovanović, S., Hočevar, K.,& Budečević, S.. (2018). Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" - National Institute of the Republic of Serbia, University of Belgrade., PP3-14.
https://hdl.handle.net/21.15107/rcub_ibiss_5058

Manitašević Jovanović S, Hočevar K, Budečević S. Flavonoid and carotenoid content in floral organs of Iris pumila. in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:PP3-14.
https://hdl.handle.net/21.15107/rcub_ibiss_5058 .

Manitašević Jovanović, Sanja, Hočevar, Katarina, Budečević, Sanja, "Flavonoid and carotenoid content in floral organs of Iris pumila" in 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):PP3-14,
https://hdl.handle.net/21.15107/rcub_ibiss_5058 .

TY  - JOUR
AU  - Souto-Vilarósa, Daniel
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Budečević, Sanja
AU  - Wang, Hui
AU  - Sapir, Yuval
AU  - Imbert, Eric
PY  - 2018
UR  - http://doi.wiley.com/10.1111/oik.04501
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2958
AB  - Community assembly rules have been extensively studied, but its association with regional environmental variation, while land use history remains largely unexplored. Land use history might be especially important in Mediterranean forests, considering their historical deforestation and recent afforestation. Using forest inventories and historical (1956) and recent (2000) land cover maps, we explored the following hypotheses: 1) woody species assembly is driven by environmental factors, but also by historical landscape attributes; 2) recent forests exhibit lower woody species richness than pre-existing due to the existence of colonization credits; 3) these credits are modulated by species’ life-forms and dispersal mechanisms. We examined the association of forest historical type (pre-existing versus recent) with total species richness and that of diverse life-forms and dispersal groups, also considering the effects of current environment and past landscape factors. When accounting for these effects, no significant differences in woody species richness were found between forest historical types except for vertebrate-dispersed species. Species richness of this group was affected by the interaction of forest historical type with distance to coast and rainfall: vertebrate-dispersed species richness increased with rainfall and distance to the coast in recent forests, while it was higher in dryer sites in pre-existing forests. In addition, forest historical types showed differences in woody species composition associated to diverse environmental and past landscape factors. In view of these results we can conclude that: 1) community assembly in terms of species richness is fast enough to exhaust most colonization credit in recent Mediterranean forests except for vertebrate-dispersed species; 2) for these species, colonization credit is affected by the interplay of forest history and a set of proxies of niche and landscape constraints of species dispersal and establishment; 3) woody species assemblage is mostly shaped by the species’ ecological niches in these forests.
T2  - Oikos
T2  - Oikos
T1  - Are pollinators the agents of selection on flower colour and size in irises?
IS  - 6
VL  - 127
DO  - 10.1111/oik.04501
SP  - 834
EP  - 846
ER  - 

@article{
author = "Souto-Vilarósa, Daniel and Vuleta, Ana and Manitašević Jovanović, Sanja and Budečević, Sanja and Wang, Hui and Sapir, Yuval and Imbert, Eric",
year = "2018",
abstract = "Community assembly rules have been extensively studied, but its association with regional environmental variation, while land use history remains largely unexplored. Land use history might be especially important in Mediterranean forests, considering their historical deforestation and recent afforestation. Using forest inventories and historical (1956) and recent (2000) land cover maps, we explored the following hypotheses: 1) woody species assembly is driven by environmental factors, but also by historical landscape attributes; 2) recent forests exhibit lower woody species richness than pre-existing due to the existence of colonization credits; 3) these credits are modulated by species’ life-forms and dispersal mechanisms. We examined the association of forest historical type (pre-existing versus recent) with total species richness and that of diverse life-forms and dispersal groups, also considering the effects of current environment and past landscape factors. When accounting for these effects, no significant differences in woody species richness were found between forest historical types except for vertebrate-dispersed species. Species richness of this group was affected by the interaction of forest historical type with distance to coast and rainfall: vertebrate-dispersed species richness increased with rainfall and distance to the coast in recent forests, while it was higher in dryer sites in pre-existing forests. In addition, forest historical types showed differences in woody species composition associated to diverse environmental and past landscape factors. In view of these results we can conclude that: 1) community assembly in terms of species richness is fast enough to exhaust most colonization credit in recent Mediterranean forests except for vertebrate-dispersed species; 2) for these species, colonization credit is affected by the interplay of forest history and a set of proxies of niche and landscape constraints of species dispersal and establishment; 3) woody species assemblage is mostly shaped by the species’ ecological niches in these forests.",
journal = "Oikos, Oikos",
title = "Are pollinators the agents of selection on flower colour and size in irises?",
number = "6",
volume = "127",
doi = "10.1111/oik.04501",
pages = "834-846"
}

Souto-Vilarósa, D., Vuleta, A., Manitašević Jovanović, S., Budečević, S., Wang, H., Sapir, Y.,& Imbert, E.. (2018). Are pollinators the agents of selection on flower colour and size in irises?. in Oikos, 127(6), 834-846.
https://doi.org/10.1111/oik.04501

Souto-Vilarósa D, Vuleta A, Manitašević Jovanović S, Budečević S, Wang H, Sapir Y, Imbert E. Are pollinators the agents of selection on flower colour and size in irises?. in Oikos. 2018;127(6):834-846.
doi:10.1111/oik.04501 .

Souto-Vilarósa, Daniel, Vuleta, Ana, Manitašević Jovanović, Sanja, Budečević, Sanja, Wang, Hui, Sapir, Yuval, Imbert, Eric, "Are pollinators the agents of selection on flower colour and size in irises?" in Oikos, 127, no. 6 (2018):834-846,
https://doi.org/10.1111/oik.04501 . .

TY  - CONF
AU  - Hočevar, Katarina
AU  - Nolić, Vladimir
AU  - Budečević, Sanja
AU  - Manitašević Jovanović, Sanja
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6204
AB  - Introduction: Natural populations of the dwarf bearded iris, Iris pumila, are markedly
polymorphic for flower color. Selection pressures exerted by a range of abiotic and
biotic factors could be involved in maintaining the polymorphism. Here we
quantified the amount of the Hsp70 and the two groups of pigments with
antioxidative properties: anthocyanins and carotenoids.These molecules impact
abiotic stress tolerance, ultimately influencing the fitness of individual plants.
Methods: A total of fifty genotypes raised in a common-garden and belonging to a
corresponding color class (yellow, blue, violet, dark blue and dark violet) were
surveyed. One fully developed leaf and a flower from each genotype were
analyzed for the Hsp70 amount and pigments concentration.
Results: The Western blot analysis revealed the presence of one isoform for the Hsp70
in leaf and two isoforms in flower organs. In both vegetative and reproductive tissues
the amount of Hsp70 was found to be the lowest in yellow colored genotypes
compared to other color classes. In violet and blue flowers, the concentration of
Hsp70 decreased gradually from light to dark colored variants. Conversely, the
concentration of anthocyanins was found to be higher in darker than in the lighter
color morphs. An inverse trend was observed for the total carotenoids
concentration.
Conclusion: This study revealed that each I. pumila color genotype produces a
unique amount of Hsp70 and antioxidative pigments in both the vegetative and
reproductive plant parts, in order to protect cellular homeostasis under fluctuating
temperature conditions prevailing in its population.
PB  - Beograd: University, Faculty of Biology
C3  - Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
T1  - Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6204
ER  - 

@conference{
author = "Hočevar, Katarina and Nolić, Vladimir and Budečević, Sanja and Manitašević Jovanović, Sanja",
year = "2017",
abstract = "Introduction: Natural populations of the dwarf bearded iris, Iris pumila, are markedly
polymorphic for flower color. Selection pressures exerted by a range of abiotic and
biotic factors could be involved in maintaining the polymorphism. Here we
quantified the amount of the Hsp70 and the two groups of pigments with
antioxidative properties: anthocyanins and carotenoids.These molecules impact
abiotic stress tolerance, ultimately influencing the fitness of individual plants.
Methods: A total of fifty genotypes raised in a common-garden and belonging to a
corresponding color class (yellow, blue, violet, dark blue and dark violet) were
surveyed. One fully developed leaf and a flower from each genotype were
analyzed for the Hsp70 amount and pigments concentration.
Results: The Western blot analysis revealed the presence of one isoform for the Hsp70
in leaf and two isoforms in flower organs. In both vegetative and reproductive tissues
the amount of Hsp70 was found to be the lowest in yellow colored genotypes
compared to other color classes. In violet and blue flowers, the concentration of
Hsp70 decreased gradually from light to dark colored variants. Conversely, the
concentration of anthocyanins was found to be higher in darker than in the lighter
color morphs. An inverse trend was observed for the total carotenoids
concentration.
Conclusion: This study revealed that each I. pumila color genotype produces a
unique amount of Hsp70 and antioxidative pigments in both the vegetative and
reproductive plant parts, in order to protect cellular homeostasis under fluctuating
temperature conditions prevailing in its population.",
publisher = "Beograd: University, Faculty of Biology",
journal = "Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia",
title = "Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6204"
}

Hočevar, K., Nolić, V., Budečević, S.,& Manitašević Jovanović, S.. (2017). Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia
Beograd: University, Faculty of Biology., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_6204

Hočevar K, Nolić V, Budečević S, Manitašević Jovanović S. Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties. in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia. 2017;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_6204 .

Hočevar, Katarina, Nolić, Vladimir, Budečević, Sanja, Manitašević Jovanović, Sanja, "Flower color morphs of Iris pumila differ in the amount of heat shock protein 70 and pigments with antioxidant properties" in Book of Abstracts: 1st Congress of Molecular Biologists of Serbia: CoMBoS; 2017 Sep 20-21; Belgrade, Serbia (2017):39,
https://hdl.handle.net/21.15107/rcub_ibiss_6204 .

TY  - JOUR
AU  - Radović, Sanja
AU  - Urošević, Aleksandar
AU  - Hočevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2017
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641600086R
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/838
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2772
AB  - The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects' landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units.
T2  - Archives of Biological Sciences
T1  - Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations
IS  - 2
VL  - 69
DO  - 10.2298/ABS160912086R
SP  - 223
EP  - 231
ER  - 

@article{
author = "Radović, Sanja and Urošević, Aleksandar and Hočevar, Katarina and Vuleta, Ana and Manitašević Jovanović, Sanja and Tucić, Branka",
year = "2017",
abstract = "The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects' landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units.",
journal = "Archives of Biological Sciences",
title = "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations",
number = "2",
volume = "69",
doi = "10.2298/ABS160912086R",
pages = "223-231"
}

Radović, S., Urošević, A., Hočevar, K., Vuleta, A., Manitašević Jovanović, S.,& Tucić, B.. (2017). Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences, 69(2), 223-231.
https://doi.org/10.2298/ABS160912086R

Radović S, Urošević A, Hočevar K, Vuleta A, Manitašević Jovanović S, Tucić B. Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences. 2017;69(2):223-231.
doi:10.2298/ABS160912086R .

Radović, Sanja, Urošević, Aleksandar, Hočevar, Katarina, Vuleta, Ana, Manitašević Jovanović, Sanja, Tucić, Branka, "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations" in Archives of Biological Sciences, 69, no. 2 (2017):223-231,
https://doi.org/10.2298/ABS160912086R . .

TY  - GEN
AU  - Radović, Sanja
AU  - Urošević, Aleksandar
AU  - Hocevar, Katarina
AU  - Vuleta, Ana
AU  - Manitašević-Jovanović, Sanja
AU  - Tucić, Branka
PY  - 2016
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641600086R
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2599
AB  - The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects’ landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units. [Projekat Ministarstva nauke Republike Srbije, br. 173007]
T2  - Archives of Biological Sciences
T1  - Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations
DO  - 10.2298/ABS160912086R
ER  - 

@misc{
author = "Radović, Sanja and Urošević, Aleksandar and Hocevar, Katarina and Vuleta, Ana and Manitašević-Jovanović, Sanja and Tucić, Branka",
year = "2016",
abstract = "The Iris flower is a complex morphological structure composed of two trimerous whorls of functionally distinct petaloid organs (the falls and the standards), one whorl of the stamens and one tricarpellary gynoecium. The petal-like style arms of the carpels are banded over the basal part of the falls, forming three pollination tunnels, each of which is perceived by the Iris pollinators as a single bilaterally symmetrical flower. Apart from the stamens, all petaloid floral organs are preferentially involved in advertising rewards to potential pollinators. Here we used the methods of geometric morphometrics to explore the shape variation in falls, standards and style arms of the Iris pumila flowers and to disentangle the symmetric and the asymmetric component of the total shape variance. Our results show that symmetric variation contributes mostly to the total shape variance in each of the three floral organs. Fluctuating asymmetry (FA) was the dominant component of the asymmetric shape variation in the falls and the standards, but appeared to be marginally significant in the style arms. The values of FA indexes for the shape of falls (insects’ landing platforms) and for the shape of standards (long-distance reward signals) were found to be two orders of magnitude greater compared to that of the style arms. Directional asymmetry appeared to be very low, but highly statistically significant for all analyzed floral organs. Because floral symmetry can reliably indicate the presence of floral rewards, an almost perfect symmetry recorded for the style arm shape might be the outcome of pollinator preferences for symmetrical pollination units. [Projekat Ministarstva nauke Republike Srbije, br. 173007]",
journal = "Archives of Biological Sciences",
title = "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations",
doi = "10.2298/ABS160912086R"
}

Radović, S., Urošević, A., Hocevar, K., Vuleta, A., Manitašević-Jovanović, S.,& Tucić, B.. (2016). Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences.
https://doi.org/10.2298/ABS160912086R

Radović S, Urošević A, Hocevar K, Vuleta A, Manitašević-Jovanović S, Tucić B. Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations. in Archives of Biological Sciences. 2016;.
doi:10.2298/ABS160912086R .

Radović, Sanja, Urošević, Aleksandar, Hocevar, Katarina, Vuleta, Ana, Manitašević-Jovanović, Sanja, Tucić, Branka, "Geometric morphometrics of functionally distinct floral organs in Iris pumila: Analyzing patterns of symmetric and asymmetric shape variations" in Archives of Biological Sciences (2016),
https://doi.org/10.2298/ABS160912086R . .