TY  - CONF
AU  - Simonović, Milica
AU  - Nestorović Živković, Jasmina
AU  - Bjedov, Ivana
AU  - Nešić, Marija
AU  - Jovanović, Vladan
AU  - Giba, Zlatko
AU  - Gašić, Uroš
AU  - Mišić, Danijela
AU  - Dmitrović, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5569
AB  - The use of bioherbicides offers the possibility to suppress harmful weeds by ecologically acceptable methods and thus increase crop productivity. Progress in understanding the mechanisms of bioherbicides action and weed defense response mechanisms, could help in defining
novel weed control strategies. This work highlights the bioherbicide potential of rhizomes of Sorghum halepense L., characterized by a high content of phenolic acids, especially p-hydroxybenzoic
acid (pHB A) and chlorogenic acid (CHL A). Methanol extract of S. halepense rhizome (SHR), and its
major bioactive principles, displayed phytotoxic effects against Chenopodium murale L., which is a
common weed of fields and roadsides. Besides reduction in C. murale seed germination, rhizome
extract of S. halepense applied in the concentrations of 0.01, 0.1, 1 and 2 mg/ml, induced changes
in the activity of antioxidant enzymes in seedlings. Catalase (CAT) activity was increased, while
peroxidase (POX) and superoxide dismutase (SOD) activities were generally decreased, with a few
exceptions (increased POX and SOD activities at 0.1 mg/ml at 0.01 mg/ml SHR, respectively), suggesting that CAT is the major responsible for the elimination of reactive oxygen species generated
during the exposure of C. murale seedlings to SHR. Application of CHL A induced changes in the
activities of antioxidant enzymes, similar to those observed on SHR treatments. The treatment
with pHB A did not significantly change the activity of POX and SOD, while CAT activity was significantly increased at all applied concentrations. These data highlight the phytotoxic potential as
a highly efficient strategy of S. halepense to invade new habitats.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract
SP  - 80
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5569
ER  - 

@conference{
author = "Simonović, Milica and Nestorović Živković, Jasmina and Bjedov, Ivana and Nešić, Marija and Jovanović, Vladan and Giba, Zlatko and Gašić, Uroš and Mišić, Danijela and Dmitrović, Slavica",
year = "2022",
abstract = "The use of bioherbicides offers the possibility to suppress harmful weeds by ecologically acceptable methods and thus increase crop productivity. Progress in understanding the mechanisms of bioherbicides action and weed defense response mechanisms, could help in defining
novel weed control strategies. This work highlights the bioherbicide potential of rhizomes of Sorghum halepense L., characterized by a high content of phenolic acids, especially p-hydroxybenzoic
acid (pHB A) and chlorogenic acid (CHL A). Methanol extract of S. halepense rhizome (SHR), and its
major bioactive principles, displayed phytotoxic effects against Chenopodium murale L., which is a
common weed of fields and roadsides. Besides reduction in C. murale seed germination, rhizome
extract of S. halepense applied in the concentrations of 0.01, 0.1, 1 and 2 mg/ml, induced changes
in the activity of antioxidant enzymes in seedlings. Catalase (CAT) activity was increased, while
peroxidase (POX) and superoxide dismutase (SOD) activities were generally decreased, with a few
exceptions (increased POX and SOD activities at 0.1 mg/ml at 0.01 mg/ml SHR, respectively), suggesting that CAT is the major responsible for the elimination of reactive oxygen species generated
during the exposure of C. murale seedlings to SHR. Application of CHL A induced changes in the
activities of antioxidant enzymes, similar to those observed on SHR treatments. The treatment
with pHB A did not significantly change the activity of POX and SOD, while CAT activity was significantly increased at all applied concentrations. These data highlight the phytotoxic potential as
a highly efficient strategy of S. halepense to invade new habitats.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract",
pages = "80",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5569"
}

Simonović, M., Nestorović Živković, J., Bjedov, I., Nešić, M., Jovanović, V., Giba, Z., Gašić, U., Mišić, D.,& Dmitrović, S.. (2022). Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 80.
https://hdl.handle.net/21.15107/rcub_ibiss_5569

Simonović M, Nestorović Živković J, Bjedov I, Nešić M, Jovanović V, Giba Z, Gašić U, Mišić D, Dmitrović S. Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:80.
https://hdl.handle.net/21.15107/rcub_ibiss_5569 .

Simonović, Milica, Nestorović Živković, Jasmina, Bjedov, Ivana, Nešić, Marija, Jovanović, Vladan, Giba, Zlatko, Gašić, Uroš, Mišić, Danijela, Dmitrović, Slavica, "Antioxidant system response of Chenopodium murale L. seedlings to phytotoxicity of Sorghum halepense L. rhizome extract" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):80,
https://hdl.handle.net/21.15107/rcub_ibiss_5569 .

TY  - CONF
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Bjedov, Ivana
AU  - Nešić, Marija
AU  - Jovanović, Vladan
AU  - Gašić, Uroš
AU  - Mišić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5572
AB  - Nowadays, one of the priorities in agricultural production is the usage of natural bioherbicides
for weed control, instead of commercial herbicides which have a strong negative effect on human
health and the environment. Due to its widespread distribution and the economic losses it causes
to the global economy, Sorghum halepense has been described as one of the world’s most invasive
plant and a dangerous weed. We here examined the possibility to exploit the phytotoxic and bioherbicide potential of this species against other weed species: Amaranthus retroflexus, Chenopodium murale and Datura stramonium. The rhizome extracts of S. halepense (concentration of 0.01,
0.1, 1, 2, and 5 mg/ml) notably reduce the final seed germination of C. murale and D. stramonium,
especially at the highest investigated concentration, while A. retroflexus seed germination was
not affected. Early seedling growth of A. retroflexus was stimulated by the rhizome extract, while in
C. murale and D. stramonium the values of growth parameters were reduced. As revealed by UHPLC/
DAD/qqqMS2
 metabolic profiling of polyphenolics, major bioactive compounds in S. halepense rhizom
extract were phenolic acids: p-hydroxybensoic acid (pHB A), chlorogenic acid (CHL A), and p-coumaric acid (pCUM A). Analysis of bioherbicide potential of these compounds, applied in a series of
concentrations (0.005, 0.01, 0.1, 0.2, and 0.3 mg/ml), revealed that pHB A, CHL A, and pCUM A, are
the main responsible for the overall effects of S. halepense rhizom on selected weeds. These results
highlight Sorghum halepense (L.) rhizomes, and their main constituents, as potent bioherbicides.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species
SP  - 121
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5572
ER  - 

@conference{
author = "Nestorović Živković, Jasmina and Dmitrović, Slavica and Bjedov, Ivana and Nešić, Marija and Jovanović, Vladan and Gašić, Uroš and Mišić, Danijela",
year = "2022",
abstract = "Nowadays, one of the priorities in agricultural production is the usage of natural bioherbicides
for weed control, instead of commercial herbicides which have a strong negative effect on human
health and the environment. Due to its widespread distribution and the economic losses it causes
to the global economy, Sorghum halepense has been described as one of the world’s most invasive
plant and a dangerous weed. We here examined the possibility to exploit the phytotoxic and bioherbicide potential of this species against other weed species: Amaranthus retroflexus, Chenopodium murale and Datura stramonium. The rhizome extracts of S. halepense (concentration of 0.01,
0.1, 1, 2, and 5 mg/ml) notably reduce the final seed germination of C. murale and D. stramonium,
especially at the highest investigated concentration, while A. retroflexus seed germination was
not affected. Early seedling growth of A. retroflexus was stimulated by the rhizome extract, while in
C. murale and D. stramonium the values of growth parameters were reduced. As revealed by UHPLC/
DAD/qqqMS2
 metabolic profiling of polyphenolics, major bioactive compounds in S. halepense rhizom
extract were phenolic acids: p-hydroxybensoic acid (pHB A), chlorogenic acid (CHL A), and p-coumaric acid (pCUM A). Analysis of bioherbicide potential of these compounds, applied in a series of
concentrations (0.005, 0.01, 0.1, 0.2, and 0.3 mg/ml), revealed that pHB A, CHL A, and pCUM A, are
the main responsible for the overall effects of S. halepense rhizom on selected weeds. These results
highlight Sorghum halepense (L.) rhizomes, and their main constituents, as potent bioherbicides.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species",
pages = "121",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5572"
}

Nestorović Živković, J., Dmitrović, S., Bjedov, I., Nešić, M., Jovanović, V., Gašić, U.,& Mišić, D.. (2022). Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 121.
https://hdl.handle.net/21.15107/rcub_ibiss_5572

Nestorović Živković J, Dmitrović S, Bjedov I, Nešić M, Jovanović V, Gašić U, Mišić D. Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:121.
https://hdl.handle.net/21.15107/rcub_ibiss_5572 .

Nestorović Živković, Jasmina, Dmitrović, Slavica, Bjedov, Ivana, Nešić, Marija, Jovanović, Vladan, Gašić, Uroš, Mišić, Danijela, "Bioherbicidal evaluation of Sorghum halepense (L.) rhizome extract and its bioactive components against selected weed species" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):121,
https://hdl.handle.net/21.15107/rcub_ibiss_5572 .

TY  - JOUR
AU  - Skočajić, Dragana
AU  - Gašić, Uroš
AU  - Dabić Zagorac, Dragana
AU  - Nešić, Marija
AU  - Tešić, Živoslav
AU  - Meland, Mekjell
AU  - Fotirić Akšić, Milica
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/12/2822
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8706243
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4759
AB  - The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars ('Amanogawa', 'Kanzan', and 'Kiku-shidare-zakura') and commercial rootstocks. Prunus avium, Prunus 'Colt', Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis 'Pyrodwarf' were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with 'Amanogawa' and 'Kiku-shidare-zakura' the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.
IS  - 12
VL  - 10
DO  - 10.3390/plants10122822
SP  - 2822
ER  - 

@article{
author = "Skočajić, Dragana and Gašić, Uroš and Dabić Zagorac, Dragana and Nešić, Marija and Tešić, Živoslav and Meland, Mekjell and Fotirić Akšić, Milica",
year = "2021",
abstract = "The aim of this study was to prove that under in vitro conditions, the adhesiveness of the callus between rootstock and scion, the development of callus cells at the points of fusion, and the presence of phenolic components are closely related to the level of (in) compatibility of the grafting combinations between Sato-zakura cherry cultivars ('Amanogawa', 'Kanzan', and 'Kiku-shidare-zakura') and commercial rootstocks. Prunus avium, Prunus 'Colt', Prunus mahaleb and Prunus serrulata were used as compatible and Prunus serotina and Pyrus communis 'Pyrodwarf' were used as two potentially incompatible rootstocks. The results indicated the significant manifestations of the early signs of the incompatibility on the callus junction. Phenols, as well as tissue senescence, were very precisely localized by toluidine blue and alcian blue as well as safranin staining, which can indicate the early signs of the callus incompatibility in some grafting unions. In the callus unions of Prunus avium with 'Amanogawa' and 'Kiku-shidare-zakura' the results of chemical analyses indicated that the existence of several flavonols, flavones and phenol acids could be involved in the incompatibility process in grafted combination. The detection of flavonol astragalin in the unions can be a biomarker of compatibility between scion and the rootstock, while some polyphenols, such as neochlorogenic acid, sinapic acid, ellagic acid, caffeic acid, baicalein, naringenin, apigenin and luteolin can be used as the indicators of graft incompatibility. p-coumaric acid and ferulic acid could be used for detection of delayed incompatibility.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.",
number = "12",
volume = "10",
doi = "10.3390/plants10122822",
pages = "2822"
}

Skočajić, D., Gašić, U., Dabić Zagorac, D., Nešić, M., Tešić, Ž., Meland, M.,& Fotirić Akšić, M.. (2021). Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.. in Plants (Basel, Switzerland)
Basel: MDPI., 10(12), 2822.
https://doi.org/10.3390/plants10122822

Skočajić D, Gašić U, Dabić Zagorac D, Nešić M, Tešić Ž, Meland M, Fotirić Akšić M. Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries.. in Plants (Basel, Switzerland). 2021;10(12):2822.
doi:10.3390/plants10122822 .

Skočajić, Dragana, Gašić, Uroš, Dabić Zagorac, Dragana, Nešić, Marija, Tešić, Živoslav, Meland, Mekjell, Fotirić Akšić, Milica, "Analysis of Phenolic Compounds for the Determination of Grafts (in) Compatibility Using In Vitro Callus Cultures of Sato-Zakura Cherries." in Plants (Basel, Switzerland), 10, no. 12 (2021):2822,
https://doi.org/10.3390/plants10122822 . .

TY  - CONF
AU  - Nešić, Marija
AU  - Avramov, Stevan
AU  - Banjanac, Tijana
AU  - Šiler, Branislav
AU  - Mišić, Danijela
AU  - Todorović, Slađana
AU  - Živković, Suzana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6186
AB  - Blackstonia perfoliata (L.) Huds. belongs to the family Gentianaceae. Due to a high content of biologically active secondary metabolites (gentiopicrin, swertiamarin and sweroside) this species has been described as a possible substitute for Centaurii Herba – a bitter remedy for treatment of digestive system disorders. Yellow wort is widespread in European and African regions of the Mediterranean and in Western Europe, mainly on calcareous grasslands habitats and fixed sand dunes. Therefore, the environmental conditions such as salinity and osmotic stress are very important for the growth and development of this species. The germination recovery from salt stress conditions of three populations of B. perfoliata collected from different natural habitats was examined. Germination rate, germination velocity and rate of germination recovery were tested. Results showed that germination characteristics depended on NaCl pre-treatment and were population-specific. Increase in salt concentration inhibited seed germination and germination recovery rate. Additionally, seeds of B. perfoliata population collected from sandy locality of Vojvodina showed different germination pattern in comparison with seeds collected from saline habitat; salt treatment improved their germination rate, but also attenuated germination velocity response.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - The effect of salt stress on yellow wort (Blackstonia perfoliata) seed germination
SP  - 176
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6186
ER  - 

@conference{
author = "Nešić, Marija and Avramov, Stevan and Banjanac, Tijana and Šiler, Branislav and Mišić, Danijela and Todorović, Slađana and Živković, Suzana",
year = "2015",
abstract = "Blackstonia perfoliata (L.) Huds. belongs to the family Gentianaceae. Due to a high content of biologically active secondary metabolites (gentiopicrin, swertiamarin and sweroside) this species has been described as a possible substitute for Centaurii Herba – a bitter remedy for treatment of digestive system disorders. Yellow wort is widespread in European and African regions of the Mediterranean and in Western Europe, mainly on calcareous grasslands habitats and fixed sand dunes. Therefore, the environmental conditions such as salinity and osmotic stress are very important for the growth and development of this species. The germination recovery from salt stress conditions of three populations of B. perfoliata collected from different natural habitats was examined. Germination rate, germination velocity and rate of germination recovery were tested. Results showed that germination characteristics depended on NaCl pre-treatment and were population-specific. Increase in salt concentration inhibited seed germination and germination recovery rate. Additionally, seeds of B. perfoliata population collected from sandy locality of Vojvodina showed different germination pattern in comparison with seeds collected from saline habitat; salt treatment improved their germination rate, but also attenuated germination velocity response.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "The effect of salt stress on yellow wort (Blackstonia perfoliata) seed germination",
pages = "176-177",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6186"
}

Nešić, M., Avramov, S., Banjanac, T., Šiler, B., Mišić, D., Todorović, S.,& Živković, S.. (2015). The effect of salt stress on yellow wort (Blackstonia perfoliata) seed germination. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 176-177.
https://hdl.handle.net/21.15107/rcub_ibiss_6186

Nešić M, Avramov S, Banjanac T, Šiler B, Mišić D, Todorović S, Živković S. The effect of salt stress on yellow wort (Blackstonia perfoliata) seed germination. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:176-177.
https://hdl.handle.net/21.15107/rcub_ibiss_6186 .

Nešić, Marija, Avramov, Stevan, Banjanac, Tijana, Šiler, Branislav, Mišić, Danijela, Todorović, Slađana, Živković, Suzana, "The effect of salt stress on yellow wort (Blackstonia perfoliata) seed germination" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):176-177,
https://hdl.handle.net/21.15107/rcub_ibiss_6186 .