TY  - JOUR
AU  - Romić, Snježana
AU  - Đorđević, Ana
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca
AU  - Bursać, Biljana
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Gligorovska, Ljupka
AU  - Korićanac, Goran
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/31974538
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3629
AB  - Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.
T2  - Food and Function
T1  - Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium-potassium pump in the hearts of male rats.
IS  - 2
VL  - 11
DO  - 10.1039/c9fo02306b
SP  - 1455
EP  - 1466
ER  - 

@article{
author = "Romić, Snježana and Đorđević, Ana and Tepavčević, Snežana and Ćulafić, Tijana and Stojiljković, Mojca and Bursać, Biljana and Stanišić, Jelena and Kostić, Milan and Gligorovska, Ljupka and Korićanac, Goran",
year = "2020",
abstract = "Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.",
journal = "Food and Function",
title = "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium-potassium pump in the hearts of male rats.",
number = "2",
volume = "11",
doi = "10.1039/c9fo02306b",
pages = "1455-1466"
}

Romić, S., Đorđević, A., Tepavčević, S., Ćulafić, T., Stojiljković, M., Bursać, B., Stanišić, J., Kostić, M., Gligorovska, L.,& Korićanac, G.. (2020). Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium-potassium pump in the hearts of male rats.. in Food and Function, 11(2), 1455-1466.
https://doi.org/10.1039/c9fo02306b

Romić S, Đorđević A, Tepavčević S, Ćulafić T, Stojiljković M, Bursać B, Stanišić J, Kostić M, Gligorovska L, Korićanac G. Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium-potassium pump in the hearts of male rats.. in Food and Function. 2020;11(2):1455-1466.
doi:10.1039/c9fo02306b .

Romić, Snježana, Đorđević, Ana, Tepavčević, Snežana, Ćulafić, Tijana, Stojiljković, Mojca, Bursać, Biljana, Stanišić, Jelena, Kostić, Milan, Gligorovska, Ljupka, Korićanac, Goran, "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium-potassium pump in the hearts of male rats." in Food and Function, 11, no. 2 (2020):1455-1466,
https://doi.org/10.1039/c9fo02306b . .

TY  - JOUR
AU  - Korićanac, G.
AU  - Đorđević, Ana
AU  - Žakula, Zorica
AU  - Vojnović-Milutinović, Danijela
AU  - Tepavčević, Snežana
AU  - Veličković, Nataša
AU  - Milosavljević, Tijana
AU  - Stojiljković, Mojca
AU  - Romić, Snježana
AU  - Matić, Gordana
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/335
AB  - We analyzed the effects of a fructose-rich diet (FRD) to test the assumption that the expression of metabolic syndrome phenotype is different in male and female rats. Two-way ANOVA revealed a significant effect of FRD on feeding behavior and carbohydrate/lipid metabolism. The increased caloric intake in FRD rats of both sexes was followed by a cluster of gender-specific changes typical for the metabolic syndrome. Female rats were characterized by decreased glycemia, increased triglycerides, enlarged visceral adipose tissue and increased absolute mass of liver, without changes in systolic blood pressure and insulin sensitivity. In contrast, male rats developed less disturbances in physical and biochemical characteristics, but blood pressure and insulin sensitivity were impaired by FRD. The results emphasize the detrimental effects of fructose consumption on cardiovascular risk and insulin action in males, whereas females are affected by other metabolic disturbances. These results support the idea of gender-dependent differences in the expression of the metabolic syndrome phenotype.
T2  - Archives of Biological Sciences
T1  - Gender modulates development of the metabolic syndrome phenotype in fructose-fed rats
IS  - 2
VL  - 65
SP  - 455
EP  - 464
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_335
ER  - 

@article{
author = "Korićanac, G. and Đorđević, Ana and Žakula, Zorica and Vojnović-Milutinović, Danijela and Tepavčević, Snežana and Veličković, Nataša and Milosavljević, Tijana and Stojiljković, Mojca and Romić, Snježana and Matić, Gordana",
year = "2013, 2013",
abstract = "We analyzed the effects of a fructose-rich diet (FRD) to test the assumption that the expression of metabolic syndrome phenotype is different in male and female rats. Two-way ANOVA revealed a significant effect of FRD on feeding behavior and carbohydrate/lipid metabolism. The increased caloric intake in FRD rats of both sexes was followed by a cluster of gender-specific changes typical for the metabolic syndrome. Female rats were characterized by decreased glycemia, increased triglycerides, enlarged visceral adipose tissue and increased absolute mass of liver, without changes in systolic blood pressure and insulin sensitivity. In contrast, male rats developed less disturbances in physical and biochemical characteristics, but blood pressure and insulin sensitivity were impaired by FRD. The results emphasize the detrimental effects of fructose consumption on cardiovascular risk and insulin action in males, whereas females are affected by other metabolic disturbances. These results support the idea of gender-dependent differences in the expression of the metabolic syndrome phenotype.",
journal = "Archives of Biological Sciences",
title = "Gender modulates development of the metabolic syndrome phenotype in fructose-fed rats",
number = "2",
volume = "65",
pages = "455-464",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_335"
}

Korićanac, G., Đorđević, A., Žakula, Z., Vojnović-Milutinović, D., Tepavčević, S., Veličković, N., Milosavljević, T., Stojiljković, M., Romić, S.,& Matić, G.. (2013). Gender modulates development of the metabolic syndrome phenotype in fructose-fed rats. in Archives of Biological Sciences, 65(2), 455-464.
https://hdl.handle.net/21.15107/rcub_ibiss_335

Korićanac G, Đorđević A, Žakula Z, Vojnović-Milutinović D, Tepavčević S, Veličković N, Milosavljević T, Stojiljković M, Romić S, Matić G. Gender modulates development of the metabolic syndrome phenotype in fructose-fed rats. in Archives of Biological Sciences. 2013;65(2):455-464.
https://hdl.handle.net/21.15107/rcub_ibiss_335 .

Korićanac, G., Đorđević, Ana, Žakula, Zorica, Vojnović-Milutinović, Danijela, Tepavčević, Snežana, Veličković, Nataša, Milosavljević, Tijana, Stojiljković, Mojca, Romić, Snježana, Matić, Gordana, "Gender modulates development of the metabolic syndrome phenotype in fructose-fed rats" in Archives of Biological Sciences, 65, no. 2 (2013):455-464,
https://hdl.handle.net/21.15107/rcub_ibiss_335 .