TY  - JOUR
AU  - Mandić, Marija
AU  - Mitić, Katarina
AU  - Nedeljković, Predrag
AU  - Perić, Mina
AU  - Božić, Bojan
AU  - Lunić, Tanja
AU  - Bačić, Ana
AU  - Rajilić-Stojanović, Mirjana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2022
UR  - https://www.mdpi.com/2072-6643/14/6/1273
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35334928
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8955508
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4940
AB  - The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.
PB  - Basel: MDPI
T2  - Nutrients
T1  - Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.
IS  - 6
VL  - 14
DO  - 10.3390/nu14061273
SP  - 1273
ER  - 

@article{
author = "Mandić, Marija and Mitić, Katarina and Nedeljković, Predrag and Perić, Mina and Božić, Bojan and Lunić, Tanja and Bačić, Ana and Rajilić-Stojanović, Mirjana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2022",
abstract = "The present study aimed to investigate the neuroprotective effects of the vitamin B complex (B1, B2, B3, B5, B6, and B12-VBC), by studying the changes in the femoral nerve, quadriceps muscle, popliteal lymph nodes and gut microbiota in the rat model of multiple sclerosis, experimental autoimmune encephalomyelitis (EAE). VBC treatment attenuated clinical signs of EAE during the disease, and reduced the duration of EAE thereby contributing to a faster recovery. In VBC-treated EAE rats, a significant decrease in nerve and muscle nuclear density was revealed during the onset period of the disease, while a marked increase was detected at the end of the disease, compared with untreated EAE rats. In the lymph nodes of VBC-treated EAE rats, a fewer number of lymphoid follicles in the cortical area and smaller epithelioid granulomas were detected. The changes in microbiota composition were examined using 16S rRNA gene sequencing and bioinformatics analysis, which revealed the potential of VBC treatment in establishing and/or maintaining gut microbiota homeostasis. Finally, the present study demonstrated that VBC treatment ameliorated the cellular changes in the affected peripheral nerve, muscles innervated by this nerve, and the gut microbiota dysbiosis which occurred during the EAE.",
publisher = "Basel: MDPI",
journal = "Nutrients",
title = "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.",
number = "6",
volume = "14",
doi = "10.3390/nu14061273",
pages = "1273"
}

Mandić, M., Mitić, K., Nedeljković, P., Perić, M., Božić, B., Lunić, T., Bačić, A., Rajilić-Stojanović, M., Peković, S.,& Božić Nedeljković, B.. (2022). Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.. in Nutrients
Basel: MDPI., 14(6), 1273.
https://doi.org/10.3390/nu14061273

Mandić M, Mitić K, Nedeljković P, Perić M, Božić B, Lunić T, Bačić A, Rajilić-Stojanović M, Peković S, Božić Nedeljković B. Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis.. in Nutrients. 2022;14(6):1273.
doi:10.3390/nu14061273 .

Mandić, Marija, Mitić, Katarina, Nedeljković, Predrag, Perić, Mina, Božić, Bojan, Lunić, Tanja, Bačić, Ana, Rajilić-Stojanović, Mirjana, Peković, Sanja, Božić Nedeljković, Biljana, "Vitamin B Complex and Experimental Autoimmune Encephalomyelitis -Attenuation of the Clinical Signs and Gut Microbiota Dysbiosis." in Nutrients, 14, no. 6 (2022):1273,
https://doi.org/10.3390/nu14061273 . .

TY  - JOUR
AU  - Lunić, Tanja M.
AU  - Oalđe, Mariana M.
AU  - Mandić, Marija R.
AU  - Sabovljević, Aneta D
AU  - Sabovljević, Marko S.
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja N.
AU  - Božić, Bojan Dj.
AU  - Božić Nedeljković, Biljana Dj.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/15/3343
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32717985
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7436104
UR  - https://radar.ibiss.bg.ac.rs/123456789/3851
AB  - Recently, there has been an increasing interest in the chemistry and biological potential of mosses, since a large number of biologically active compounds have been found within these species. This study aimed at examining the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory/antineurodegenerative, and antitumor activities) of moss Hypnum cupressiforme Hedw. extracts. Corresponding extracts have been obtained applying Soxhlet extractor. The chemical characterization was performed using spectrophotometric assays and liquid chromatography-mass spectrometry (LC-MS). The extracts were analyzed for antioxidant activity and for inhibitory activities on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase. Additionally, extracts were tested against four cell lines-MRC-5, BV2, HCT-116, and MDA-MB-231-for antitumor and anti-inflammatory activities. Chemical analysis of extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. Major compounds identified by LC-MS in H. cupressiforme were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. According to biochemical assays the investigated extracts exhibited significant immunomodulatory potential. Significant antiproliferative potential against MDA-MB-231 cells has been observed together with the promising anti-neuroinflammatory application. The obtained data suggest that moss H. cupressiforme is a valuable natural source of biologically active compounds with potential application in the pharmaceutical industry.
PB  - MDPI AG
T2  - Molecules (Basel, Switzerland)
T1  - Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.
IS  - 15
VL  - 25
DO  - 10.3390/molecules25153343
SP  - 3343
ER  - 

@article{
author = "Lunić, Tanja M. and Oalđe, Mariana M. and Mandić, Marija R. and Sabovljević, Aneta D and Sabovljević, Marko S. and Gašić, Uroš and Duletić-Laušević, Sonja N. and Božić, Bojan Dj. and Božić Nedeljković, Biljana Dj.",
year = "2020",
abstract = "Recently, there has been an increasing interest in the chemistry and biological potential of mosses, since a large number of biologically active compounds have been found within these species. This study aimed at examining the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory/antineurodegenerative, and antitumor activities) of moss Hypnum cupressiforme Hedw. extracts. Corresponding extracts have been obtained applying Soxhlet extractor. The chemical characterization was performed using spectrophotometric assays and liquid chromatography-mass spectrometry (LC-MS). The extracts were analyzed for antioxidant activity and for inhibitory activities on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase. Additionally, extracts were tested against four cell lines-MRC-5, BV2, HCT-116, and MDA-MB-231-for antitumor and anti-inflammatory activities. Chemical analysis of extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. Major compounds identified by LC-MS in H. cupressiforme were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. According to biochemical assays the investigated extracts exhibited significant immunomodulatory potential. Significant antiproliferative potential against MDA-MB-231 cells has been observed together with the promising anti-neuroinflammatory application. The obtained data suggest that moss H. cupressiforme is a valuable natural source of biologically active compounds with potential application in the pharmaceutical industry.",
publisher = "MDPI AG",
journal = "Molecules (Basel, Switzerland)",
title = "Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.",
number = "15",
volume = "25",
doi = "10.3390/molecules25153343",
pages = "3343"
}

Lunić, T. M., Oalđe, M. M., Mandić, M. R., Sabovljević, A. D., Sabovljević, M. S., Gašić, U., Duletić-Laušević, S. N., Božić, B. Dj.,& Božić Nedeljković, B. Dj.. (2020). Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.. in Molecules (Basel, Switzerland)
MDPI AG., 25(15), 3343.
https://doi.org/10.3390/molecules25153343

Lunić TM, Oalđe MM, Mandić MR, Sabovljević AD, Sabovljević MS, Gašić U, Duletić-Laušević SN, Božić BD, Božić Nedeljković BD. Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw.. in Molecules (Basel, Switzerland). 2020;25(15):3343.
doi:10.3390/molecules25153343 .

Lunić, Tanja M., Oalđe, Mariana M., Mandić, Marija R., Sabovljević, Aneta D, Sabovljević, Marko S., Gašić, Uroš, Duletić-Laušević, Sonja N., Božić, Bojan Dj., Božić Nedeljković, Biljana Dj., "Extracts Characterization and In Vitro Evaluation of Potential Immunomodulatory Activities of the Moss Hypnum cupressiforme Hedw." in Molecules (Basel, Switzerland), 25, no. 15 (2020):3343,
https://doi.org/10.3390/molecules25153343 . .

TY  - CONF
AU  - Mandić, Marija
AU  - Oalđe, Mariana
AU  - Lunić, Tanja
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja
AU  - Božić, Bojan
AU  - Božić Nedeljković, Biljana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4105
AB  - Owing to structural diversity and potental pharmacological activity, bioactive compounds derived from natural sources are in the focus of intensive exploration. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. was examined, and further tested for antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative activity in vitro. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical analysis revealed the presence of phenolics, flavonoids, and triterpenoids as secondary metabolites of high biological activity. Antioxidant activity of extracts was assessed using three different methods, DPPH (2,2-diphenyl-1-picrylhydrazyl), total reducing power assay, and β-carotene/linoleic acid assay. To evaluate the enzyme-inhibitory activity, extracts were screened for α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase inhibitory effects. The biocompatibility of moss extracts was confirmed using normal human fibroblast cell line, MRC-5. The H. ciliata extracts displayed significant antiproliferative activity (~ 50% inhibition) against the MDA-MB-231 (human breast adenocarcinoma) cells, which had not previously been reported elsewhere. The potential anti-neuroinflammatory activity was observed due to significant reduction of the NO production by LPS-stimulated BV2 (normal murine microglia cell line), being in line with previously recognized antineurodegenerative potential, as measured by the inhibition of acetylcholinesterase and tyrosinase activity. Furthermore, the H. ciliata extracts displayed significant antidiabetic effect via α-glucosidase inhibition. In general, the obtained results suggest the potential immunomodulatory activity of the moss H. ciliata extracts, remaining to be characterized in more detail in search for mechanisms underlying the immunomodulation processes.
PB  - Basel: MDPI
C3  - 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online
T1  - Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential
DO  - 10.3390/ECMC2020-07281
ER  - 

@conference{
author = "Mandić, Marija and Oalđe, Mariana and Lunić, Tanja and Sabovljević, Aneta and Sabovljević, Marko and Gašić, Uroš and Duletić-Laušević, Sonja and Božić, Bojan and Božić Nedeljković, Biljana",
year = "2020",
abstract = "Owing to structural diversity and potental pharmacological activity, bioactive compounds derived from natural sources are in the focus of intensive exploration. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. was examined, and further tested for antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative activity in vitro. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical analysis revealed the presence of phenolics, flavonoids, and triterpenoids as secondary metabolites of high biological activity. Antioxidant activity of extracts was assessed using three different methods, DPPH (2,2-diphenyl-1-picrylhydrazyl), total reducing power assay, and β-carotene/linoleic acid assay. To evaluate the enzyme-inhibitory activity, extracts were screened for α-amylase, α-glucosidase, acetylcholinesterase, and tyrosinase inhibitory effects. The biocompatibility of moss extracts was confirmed using normal human fibroblast cell line, MRC-5. The H. ciliata extracts displayed significant antiproliferative activity (~ 50% inhibition) against the MDA-MB-231 (human breast adenocarcinoma) cells, which had not previously been reported elsewhere. The potential anti-neuroinflammatory activity was observed due to significant reduction of the NO production by LPS-stimulated BV2 (normal murine microglia cell line), being in line with previously recognized antineurodegenerative potential, as measured by the inhibition of acetylcholinesterase and tyrosinase activity. Furthermore, the H. ciliata extracts displayed significant antidiabetic effect via α-glucosidase inhibition. In general, the obtained results suggest the potential immunomodulatory activity of the moss H. ciliata extracts, remaining to be characterized in more detail in search for mechanisms underlying the immunomodulation processes.",
publisher = "Basel: MDPI",
journal = "6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online",
title = "Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential",
doi = "10.3390/ECMC2020-07281"
}

Mandić, M., Oalđe, M., Lunić, T., Sabovljević, A., Sabovljević, M., Gašić, U., Duletić-Laušević, S., Božić, B.,& Božić Nedeljković, B.. (2020). Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online
Basel: MDPI..
https://doi.org/10.3390/ECMC2020-07281

Mandić M, Oalđe M, Lunić T, Sabovljević A, Sabovljević M, Gašić U, Duletić-Laušević S, Božić B, Božić Nedeljković B. Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online. 2020;.
doi:10.3390/ECMC2020-07281 .

Mandić, Marija, Oalđe, Mariana, Lunić, Tanja, Sabovljević, Aneta, Sabovljević, Marko, Gašić, Uroš, Duletić-Laušević, Sonja, Božić, Bojan, Božić Nedeljković, Biljana, "Hedwigia ciliata (Hedw.) P. Beauv. moss extracts - Chemical characterization and in vitro testing of immunomodulatory potential" in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online (2020),
https://doi.org/10.3390/ECMC2020-07281 . .