TY  - JOUR
AU  - Boroja, Tatjana
AU  - Katanić, Jelena
AU  - Rosić, Gvozden
AU  - Selaković, Dragica
AU  - Joksimović, Jovana
AU  - Mišić, Danijela
AU  - Stanković, Vesna
AU  - Jovičić, Nemanja
AU  - Mihailović, Vladimir
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0278691518302904?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3066
AB  - The aim of our study was to examine the potential ameliorating effect of the methanolic extract of Satureja hortensis L. (summer savory) aerial parts against cisplatin-induced oxidative damage in renal, hepatic, and testicular tissues. S. hortensis methanol extract at the doses of 50, 100 and 200 mg/kg of body weight were orally administered to Wistar rats once daily for 10 days. Toxicity was induced by intraperitoneal injection of a single dose of cisplatin (7.5 mg/kg of body weight) on the 5th day of the experiment. Applied treatment with S. hortensis extract restored tissue morphology, ameliorated levels of serum parameters for liver, renal and testes function, tissue oxidative stress parameters, and increased Bcl-2/Bax ratio as an indicator of apoptosis in experimental animals caused by application of cisplatin. UHPLC/DAD/HESI-MS/MS analysis revealed that S. hortensis extract was rich in phenolic compounds with rosmarinic acid (24.9 mg/g) as the main compound, followed by caffeic acid (1.28 mg/g) and naringenin (1.06 mg/g). Our findings suggest that S. hortensis may be a valuable source of dietary and pharmacologically important phenolic compounds, especially rosmarinic acid, in pharmaceutical and functional food formulations in order to maintain normal health conditions or as a remedy in various diseases caused by oxidative damage.
T2  - Food and Chemical Toxicology
T1  - Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity.
VL  - 118
DO  - 10.1016/j.fct.2018.05.001
SP  - 252
EP  - 263
ER  - 

@article{
author = "Boroja, Tatjana and Katanić, Jelena and Rosić, Gvozden and Selaković, Dragica and Joksimović, Jovana and Mišić, Danijela and Stanković, Vesna and Jovičić, Nemanja and Mihailović, Vladimir",
year = "2018",
abstract = "The aim of our study was to examine the potential ameliorating effect of the methanolic extract of Satureja hortensis L. (summer savory) aerial parts against cisplatin-induced oxidative damage in renal, hepatic, and testicular tissues. S. hortensis methanol extract at the doses of 50, 100 and 200 mg/kg of body weight were orally administered to Wistar rats once daily for 10 days. Toxicity was induced by intraperitoneal injection of a single dose of cisplatin (7.5 mg/kg of body weight) on the 5th day of the experiment. Applied treatment with S. hortensis extract restored tissue morphology, ameliorated levels of serum parameters for liver, renal and testes function, tissue oxidative stress parameters, and increased Bcl-2/Bax ratio as an indicator of apoptosis in experimental animals caused by application of cisplatin. UHPLC/DAD/HESI-MS/MS analysis revealed that S. hortensis extract was rich in phenolic compounds with rosmarinic acid (24.9 mg/g) as the main compound, followed by caffeic acid (1.28 mg/g) and naringenin (1.06 mg/g). Our findings suggest that S. hortensis may be a valuable source of dietary and pharmacologically important phenolic compounds, especially rosmarinic acid, in pharmaceutical and functional food formulations in order to maintain normal health conditions or as a remedy in various diseases caused by oxidative damage.",
journal = "Food and Chemical Toxicology",
title = "Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity.",
volume = "118",
doi = "10.1016/j.fct.2018.05.001",
pages = "252-263"
}

Boroja, T., Katanić, J., Rosić, G., Selaković, D., Joksimović, J., Mišić, D., Stanković, V., Jovičić, N.,& Mihailović, V.. (2018). Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity.. in Food and Chemical Toxicology, 118, 252-263.
https://doi.org/10.1016/j.fct.2018.05.001

Boroja T, Katanić J, Rosić G, Selaković D, Joksimović J, Mišić D, Stanković V, Jovičić N, Mihailović V. Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity.. in Food and Chemical Toxicology. 2018;118:252-263.
doi:10.1016/j.fct.2018.05.001 .

Boroja, Tatjana, Katanić, Jelena, Rosić, Gvozden, Selaković, Dragica, Joksimović, Jovana, Mišić, Danijela, Stanković, Vesna, Jovičić, Nemanja, Mihailović, Vladimir, "Summer savory (Satureja hortensis L.) extract: Phytochemical profile and modulation of cisplatin-induced liver, renal and testicular toxicity." in Food and Chemical Toxicology, 118 (2018):252-263,
https://doi.org/10.1016/j.fct.2018.05.001 . .

TY  - JOUR
AU  - Katanić, Jelena
AU  - Matić, Sanja
AU  - Pferschy-Wenzig, Eva-Maria
AU  - Kretschmer, Nadine
AU  - Boroja, Tatjana
AU  - Mihailović, Vladimir
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Bauer, Rudolf
PY  - 2017
UR  - https://www.sciencedirect.com/science/article/pii/S0278691516304343?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3178
AB  - Filipendula ulmaria, known as meadowsweet, is a perennial herb found in wild and cultivated habitats in Europe and Asia. Usage of F. ulmaria in traditional medicine is based on diuretic, astringent, antirheumatic, and anti-inflammatory properties of this plant. Exposure to cisplatin at a dose of 7.5 mg/kg caused significant increase in serum parameters of liver and kidneys function and tissue oxidative stress markers along with some histopathological changes in liver and kidney tissues of experimental rats, as well as high level of genotoxicity. Administration of F. ulmaria extracts in three different concentrations (100, 200, and 400 mg/kg/day) for 10 days resulted in a reduction of oxidative stress in tissues and decrease of serum parameters. Moreover, tested extracts attenuated the genotoxicity of cisplatin in reverse dose-dependent manner. F. ulmaria extracts had no in vitro cytotoxic activity at all applied concentrations (IC50 > 50 μg/mL). Tested extracts, rich in polyphenolic compounds, attenuate cisplatin-induced liver and kidney oxidative stress, reduce tissue damage, and enhance the antioxidative status of experimental animals during cisplatin application. Therefore, F. ulmaria extracts may be used as supportive agent for the prevention and amelioration of cisplatin side effects.
T2  - Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association
T1  - Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.
VL  - 99
DO  - 10.1016/j.fct.2016.11.018
SP  - 86
EP  - 102
ER  - 

@article{
author = "Katanić, Jelena and Matić, Sanja and Pferschy-Wenzig, Eva-Maria and Kretschmer, Nadine and Boroja, Tatjana and Mihailović, Vladimir and Stanković, Vesna and Stanković, Nevena and Mladenović, Milan and Stanić, Snežana and Mihailović, Mirjana and Bauer, Rudolf",
year = "2017",
abstract = "Filipendula ulmaria, known as meadowsweet, is a perennial herb found in wild and cultivated habitats in Europe and Asia. Usage of F. ulmaria in traditional medicine is based on diuretic, astringent, antirheumatic, and anti-inflammatory properties of this plant. Exposure to cisplatin at a dose of 7.5 mg/kg caused significant increase in serum parameters of liver and kidneys function and tissue oxidative stress markers along with some histopathological changes in liver and kidney tissues of experimental rats, as well as high level of genotoxicity. Administration of F. ulmaria extracts in three different concentrations (100, 200, and 400 mg/kg/day) for 10 days resulted in a reduction of oxidative stress in tissues and decrease of serum parameters. Moreover, tested extracts attenuated the genotoxicity of cisplatin in reverse dose-dependent manner. F. ulmaria extracts had no in vitro cytotoxic activity at all applied concentrations (IC50 > 50 μg/mL). Tested extracts, rich in polyphenolic compounds, attenuate cisplatin-induced liver and kidney oxidative stress, reduce tissue damage, and enhance the antioxidative status of experimental animals during cisplatin application. Therefore, F. ulmaria extracts may be used as supportive agent for the prevention and amelioration of cisplatin side effects.",
journal = "Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association",
title = "Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.",
volume = "99",
doi = "10.1016/j.fct.2016.11.018",
pages = "86-102"
}

Katanić, J., Matić, S., Pferschy-Wenzig, E., Kretschmer, N., Boroja, T., Mihailović, V., Stanković, V., Stanković, N., Mladenović, M., Stanić, S., Mihailović, M.,& Bauer, R.. (2017). Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.. in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association, 99, 86-102.
https://doi.org/10.1016/j.fct.2016.11.018

Katanić J, Matić S, Pferschy-Wenzig E, Kretschmer N, Boroja T, Mihailović V, Stanković V, Stanković N, Mladenović M, Stanić S, Mihailović M, Bauer R. Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.. in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association. 2017;99:86-102.
doi:10.1016/j.fct.2016.11.018 .

Katanić, Jelena, Matić, Sanja, Pferschy-Wenzig, Eva-Maria, Kretschmer, Nadine, Boroja, Tatjana, Mihailović, Vladimir, Stanković, Vesna, Stanković, Nevena, Mladenović, Milan, Stanić, Snežana, Mihailović, Mirjana, Bauer, Rudolf, "Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis." in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association, 99 (2017):86-102,
https://doi.org/10.1016/j.fct.2016.11.018 . .

TY  - GEN
AU  - Katanić, Jelena
AU  - Mihailović, Vladimir
AU  - Matić, Sanja
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Mladenović, Milan
AU  - Stanić, Snežana
AU  - Kreft, Samo
AU  - Mihailović, Mirjana
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1756464616303644
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2841
AB  - The authors regret that in Fig. 3 inadvertently was incorporated two photographs of the same tissue preparation of group VII (Fig. 3VII and X). They would like to inform that this wrong figure did not change the results of their study. The accurate representative photograph of kidney sections of experimental animals from group X (Fig. 3X in Fig. 3) is given below. The authors would like to apologize for any inconvenience caused.
T2  - Journal of Functional Foods
T1  - Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]
VL  - 28
DO  - 10.1016/j.jff.2016.11.017
SP  - 326
EP  - 327
ER  - 

@misc{
author = "Katanić, Jelena and Mihailović, Vladimir and Matić, Sanja and Stanković, Vesna and Stanković, Nevena and Boroja, Tatjana and Mladenović, Milan and Stanić, Snežana and Kreft, Samo and Mihailović, Mirjana",
year = "2017",
abstract = "The authors regret that in Fig. 3 inadvertently was incorporated two photographs of the same tissue preparation of group VII (Fig. 3VII and X). They would like to inform that this wrong figure did not change the results of their study. The accurate representative photograph of kidney sections of experimental animals from group X (Fig. 3X in Fig. 3) is given below. The authors would like to apologize for any inconvenience caused.",
journal = "Journal of Functional Foods",
title = "Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]",
volume = "28",
doi = "10.1016/j.jff.2016.11.017",
pages = "326-327"
}

Katanić, J., Mihailović, V., Matić, S., Stanković, V., Stanković, N., Boroja, T., Mladenović, M., Stanić, S., Kreft, S.,& Mihailović, M.. (2017). Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]. in Journal of Functional Foods, 28, 326-327.
https://doi.org/10.1016/j.jff.2016.11.017

Katanić J, Mihailović V, Matić S, Stanković V, Stanković N, Boroja T, Mladenović M, Stanić S, Kreft S, Mihailović M. Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]. in Journal of Functional Foods. 2017;28:326-327.
doi:10.1016/j.jff.2016.11.017 .

Katanić, Jelena, Mihailović, Vladimir, Matić, Sanja, Stanković, Vesna, Stanković, Nevena, Boroja, Tatjana, Mladenović, Milan, Stanić, Snežana, Kreft, Samo, Mihailović, Mirjana, "Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]" in Journal of Functional Foods, 28 (2017):326-327,
https://doi.org/10.1016/j.jff.2016.11.017 . .

TY  - CONF
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Boroja, Tatjana
AU  - Vuković, Nenad
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6144
AB  - Eight chroman-2,4-diones, namely 2a-h, were evaluated as in vivo genotoxic agents in Wistar rats livers and kidneys using the alkaline comet assay. Compounds 2a, (E)-3- (1-(2-aminoethylamino) ethylidene) chroman-2,4-dione,2b,(E)-3-( 1-(2-hydroxyethylamino) ethylidene) chroman-2,4-dione, and 2f, (3E,3'E) - 3,3'-( l, l '-(ethane-1,2-diylbis (azanediyl)) bis (ethan-1-yl-l-ylidene)) dichroman-2,4- dione showed no genotoxic potential and were tested as antigenotoxic agents by application prior to ethyl methanesulfonate (EMS), a proven mutagen. As antigentotoxics, compounds significantly diminished EMS-induced DNA damage in both organs. The reduction of liver DNA damage amounted 86.93% (2b), 77.23% (2f), and 64.52% (2a), respectively, while the reduction in kidney DNA damage was 89.52 (2b), 82.50% (2f) and 68.14% (2a). Since EMS produce harmful d-ethylguanine lesion which is incorporated in aberrant genotoxic G=T and T=G pairing after rat Topoisomerase Ila (rToplla) catalyzed ATP-dependent DNA strand breaks, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on enzyme level using molecular docking and molecular dynamics simulations. According to molecular docking studies, those compounds occupy the A TPase region proximal to rGlu86, catalytic amino acid involved in the hydrolysis of y-pbosphate group of ATP via water bridges. Molecular dynamics simulations showed that 2a, 2b, and 2f are a barrier for the formation of ATP-H20-rGlu86 bridge. Since compounds inhibit the hydrolysis of ATP, they prohibit the energy for the DNA double strand ligation, and therefore neutralize any possible damage that can arise after the formation of 06-ethylguanine harmful lesion. Consequently, compounds 2a, 2b, and 2f prevent EMS mutagenic and carcinogenic effects, and can be applied in the cancer treatment to control the rate of anticancer alkylation drugs.
PB  - Banja Luka: Prirodno-matematički fakultet
C3  - III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska
T1  - Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach
SP  - 118
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6144
ER  - 

@conference{
author = "Stanković, Nevena and Mladenović, Milan and Matić, Sanja and Stanić, Snežana and Mihailović, Mirjana and Mihailović, Vladimir and Katanić, Jelena and Boroja, Tatjana and Vuković, Nenad",
year = "2015",
abstract = "Eight chroman-2,4-diones, namely 2a-h, were evaluated as in vivo genotoxic agents in Wistar rats livers and kidneys using the alkaline comet assay. Compounds 2a, (E)-3- (1-(2-aminoethylamino) ethylidene) chroman-2,4-dione,2b,(E)-3-( 1-(2-hydroxyethylamino) ethylidene) chroman-2,4-dione, and 2f, (3E,3'E) - 3,3'-( l, l '-(ethane-1,2-diylbis (azanediyl)) bis (ethan-1-yl-l-ylidene)) dichroman-2,4- dione showed no genotoxic potential and were tested as antigenotoxic agents by application prior to ethyl methanesulfonate (EMS), a proven mutagen. As antigentotoxics, compounds significantly diminished EMS-induced DNA damage in both organs. The reduction of liver DNA damage amounted 86.93% (2b), 77.23% (2f), and 64.52% (2a), respectively, while the reduction in kidney DNA damage was 89.52 (2b), 82.50% (2f) and 68.14% (2a). Since EMS produce harmful d-ethylguanine lesion which is incorporated in aberrant genotoxic G=T and T=G pairing after rat Topoisomerase Ila (rToplla) catalyzed ATP-dependent DNA strand breaks, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on enzyme level using molecular docking and molecular dynamics simulations. According to molecular docking studies, those compounds occupy the A TPase region proximal to rGlu86, catalytic amino acid involved in the hydrolysis of y-pbosphate group of ATP via water bridges. Molecular dynamics simulations showed that 2a, 2b, and 2f are a barrier for the formation of ATP-H20-rGlu86 bridge. Since compounds inhibit the hydrolysis of ATP, they prohibit the energy for the DNA double strand ligation, and therefore neutralize any possible damage that can arise after the formation of 06-ethylguanine harmful lesion. Consequently, compounds 2a, 2b, and 2f prevent EMS mutagenic and carcinogenic effects, and can be applied in the cancer treatment to control the rate of anticancer alkylation drugs.",
publisher = "Banja Luka: Prirodno-matematički fakultet",
journal = "III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska",
title = "Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach",
pages = "118",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6144"
}

Stanković, N., Mladenović, M., Matić, S., Stanić, S., Mihailović, M., Mihailović, V., Katanić, J., Boroja, T.,& Vuković, N.. (2015). Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach. in III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska
Banja Luka: Prirodno-matematički fakultet., 118.
https://hdl.handle.net/21.15107/rcub_ibiss_6144

Stanković N, Mladenović M, Matić S, Stanić S, Mihailović M, Mihailović V, Katanić J, Boroja T, Vuković N. Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach. in III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska. 2015;:118.
https://hdl.handle.net/21.15107/rcub_ibiss_6144 .

Stanković, Nevena, Mladenović, Milan, Matić, Sanja, Stanić, Snežana, Mihailović, Mirjana, Mihailović, Vladimir, Katanić, Jelena, Boroja, Tatjana, Vuković, Nenad, "Newly discovered chroman-2,4-diones neutralize DNA alkylation damage in vivo on topIIa level: A story behind the molecular modeling approach" in III simpozijum biologa i ekologa Republike Srpske (SBERS, 2015): Zbornik radova; 2015 Nov 12-14; Banja Luka, Republika Srpska (2015):118,
https://hdl.handle.net/21.15107/rcub_ibiss_6144 .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Boroja, Tatjana
AU  - Vuković, Nenad
PY  - 2015
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84943457635&partnerID=tZOtx3y1
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0006295215005511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3175
AB  - Eight chroman-2,4-diones, namely 2a-h, previously investigated as anticoagulants, of which 2a and 2f as the most active, were evaluated as in vivo genotoxic agents in Wistar rat livers and kidneys using the comet assay. Compounds 2a, 2b, and 2f without genotoxic activity were applied prior to ethyl methanesulfonate (EMS) and diminished EMS-induced DNA damage according to the total score and percentage of reduction. EMS produce harmful O(6)-ethylguanine lesion which is incorporated in aberrant genotoxic GT and TG pairing after ATP-dependent DNA strand breaks have been catalyzed by rat Topoisomerase IIα (rTopIIα, EC 5.99.1.3). Therefore, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on the enzyme level using molecular docking and molecular dynamics simulations insamuch as it had been determined that compounds do not intercalate DNA but instead inhibit the ATPase activity. Calculations predicted that compounds inhibit ATP hydrolysis before the DNA-EMS cleavage is being catalyzed by rTopIIα, prevent EMS mutagenic and carcinogenic effects, and beside anticoagulant activity can even be applied in the cancer treatment to control the rate of anticancer alkylation drugs.
PB  - Elsevier
T2  - Biochemical Pharmacology
T1  - Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach
IS  - 1
VL  - 98
DO  - 10.1016/j.bcp.2015.08.106
SP  - 243
EP  - 266
ER  - 

@article{
author = "Mladenović, Milan and Stanković, Nevena and Matić, Sanja and Stanić, Snežana and Mihailović, Mirjana and Mihailović, Vladimir and Katanić, Jelena and Boroja, Tatjana and Vuković, Nenad",
year = "2015",
abstract = "Eight chroman-2,4-diones, namely 2a-h, previously investigated as anticoagulants, of which 2a and 2f as the most active, were evaluated as in vivo genotoxic agents in Wistar rat livers and kidneys using the comet assay. Compounds 2a, 2b, and 2f without genotoxic activity were applied prior to ethyl methanesulfonate (EMS) and diminished EMS-induced DNA damage according to the total score and percentage of reduction. EMS produce harmful O(6)-ethylguanine lesion which is incorporated in aberrant genotoxic GT and TG pairing after ATP-dependent DNA strand breaks have been catalyzed by rat Topoisomerase IIα (rTopIIα, EC 5.99.1.3). Therefore, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on the enzyme level using molecular docking and molecular dynamics simulations insamuch as it had been determined that compounds do not intercalate DNA but instead inhibit the ATPase activity. Calculations predicted that compounds inhibit ATP hydrolysis before the DNA-EMS cleavage is being catalyzed by rTopIIα, prevent EMS mutagenic and carcinogenic effects, and beside anticoagulant activity can even be applied in the cancer treatment to control the rate of anticancer alkylation drugs.",
publisher = "Elsevier",
journal = "Biochemical Pharmacology",
title = "Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach",
number = "1",
volume = "98",
doi = "10.1016/j.bcp.2015.08.106",
pages = "243-266"
}

Mladenović, M., Stanković, N., Matić, S., Stanić, S., Mihailović, M., Mihailović, V., Katanić, J., Boroja, T.,& Vuković, N.. (2015). Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach. in Biochemical Pharmacology
Elsevier., 98(1), 243-266.
https://doi.org/10.1016/j.bcp.2015.08.106

Mladenović M, Stanković N, Matić S, Stanić S, Mihailović M, Mihailović V, Katanić J, Boroja T, Vuković N. Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach. in Biochemical Pharmacology. 2015;98(1):243-266.
doi:10.1016/j.bcp.2015.08.106 .

Mladenović, Milan, Stanković, Nevena, Matić, Sanja, Stanić, Snežana, Mihailović, Mirjana, Mihailović, Vladimir, Katanić, Jelena, Boroja, Tatjana, Vuković, Nenad, "Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach" in Biochemical Pharmacology, 98, no. 1 (2015):243-266,
https://doi.org/10.1016/j.bcp.2015.08.106 . .

TY  - JOUR
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Stanković, Vesna
AU  - Mihailović, Mirjana
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Boroja, Tatjana
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2004
AB  - Two chroman-2,4-dione derivatives, namely 2a and 2f, were tested as in
   vivo anticoagulants by seven days of continuous per os application to
   adult male Wistar rats in a concentration of 20 mg/kg of body weight.
   Derivatives were selected from a group of six previously
   intraperitoneally applied compounds on the basis of presenting
   remarkable activity in a concentration of 2 mg/kg of body weight. The
   derivatives 2a and 2f are VKORC1 inhibitors, and comparison of the
   absorption spectra, association, and dissociation constants suggested
   that the compounds will be bound to serum albumin in the same manner as
   warfarin is, leading to transfer towards the molecular target VKORC1.
   After oral administration, the compounds proved to be anticoagulants
   comparable with warfarin, inasmuch as the measured prothrombin times for
   2a and 2f were 56.63 and 60.08 s, respectively. The INR values of 2a and
   2f ranged from 2.6 to 2.8, recommending them as useful therapeutics in
   the treatment of patients suffering from thromboembolic events and
   atrial fibrillation. The high percentage of binding and high binding
   affinity of 2a and 2f towards serum albumin reduced the risk of induced
   internal bleeding. Several kinds of toxicity studies were performed to
   investigate whether or not 2a and 2f can cause pathological changes in
   the liver, kidneys, and DNA. The catalytic activity of serum enzymes,
   concentration and catalytic activity of liver and kidney oxidative
   stress markers and enzymes, respectively, as well as the observed
   hepatic and renal morphological changes indicated that the compounds in
   relation to warfarin induced irrelevant hepatic toxicity, no increment
   of necrosis, and inconsiderable oxidative damage in the liver and
   kidneys. Estimation of DNA damage using the comet assay confirmed that
   2a and 2f caused no clinically significant genotoxicity. The higher
   activity and lower toxicity of 2f recommended this compound as a better
   drug candidate than 2a. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants
VL  - 227
DO  - 10.1016/j.cbi.2014.12.005
SP  - 18
EP  - 31
ER  - 

@article{
author = "Stanković, Nevena and Mladenović, Milan and Matić, Sanja and Stanić, Snežana and Stanković, Vesna and Mihailović, Mirjana and Mihailović, Vladimir and Katanić, Jelena and Boroja, Tatjana and Vuković, Nenad and Sukdolak, Slobodan",
year = "2015",
abstract = "Two chroman-2,4-dione derivatives, namely 2a and 2f, were tested as in
   vivo anticoagulants by seven days of continuous per os application to
   adult male Wistar rats in a concentration of 20 mg/kg of body weight.
   Derivatives were selected from a group of six previously
   intraperitoneally applied compounds on the basis of presenting
   remarkable activity in a concentration of 2 mg/kg of body weight. The
   derivatives 2a and 2f are VKORC1 inhibitors, and comparison of the
   absorption spectra, association, and dissociation constants suggested
   that the compounds will be bound to serum albumin in the same manner as
   warfarin is, leading to transfer towards the molecular target VKORC1.
   After oral administration, the compounds proved to be anticoagulants
   comparable with warfarin, inasmuch as the measured prothrombin times for
   2a and 2f were 56.63 and 60.08 s, respectively. The INR values of 2a and
   2f ranged from 2.6 to 2.8, recommending them as useful therapeutics in
   the treatment of patients suffering from thromboembolic events and
   atrial fibrillation. The high percentage of binding and high binding
   affinity of 2a and 2f towards serum albumin reduced the risk of induced
   internal bleeding. Several kinds of toxicity studies were performed to
   investigate whether or not 2a and 2f can cause pathological changes in
   the liver, kidneys, and DNA. The catalytic activity of serum enzymes,
   concentration and catalytic activity of liver and kidney oxidative
   stress markers and enzymes, respectively, as well as the observed
   hepatic and renal morphological changes indicated that the compounds in
   relation to warfarin induced irrelevant hepatic toxicity, no increment
   of necrosis, and inconsiderable oxidative damage in the liver and
   kidneys. Estimation of DNA damage using the comet assay confirmed that
   2a and 2f caused no clinically significant genotoxicity. The higher
   activity and lower toxicity of 2f recommended this compound as a better
   drug candidate than 2a. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants",
volume = "227",
doi = "10.1016/j.cbi.2014.12.005",
pages = "18-31"
}

Stanković, N., Mladenović, M., Matić, S., Stanić, S., Stanković, V., Mihailović, M., Mihailović, V., Katanić, J., Boroja, T., Vuković, N.,& Sukdolak, S.. (2015). Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants. in Chemico-Biological Interactions
Elsevier., 227, 18-31.
https://doi.org/10.1016/j.cbi.2014.12.005

Stanković N, Mladenović M, Matić S, Stanić S, Stanković V, Mihailović M, Mihailović V, Katanić J, Boroja T, Vuković N, Sukdolak S. Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants. in Chemico-Biological Interactions. 2015;227:18-31.
doi:10.1016/j.cbi.2014.12.005 .

Stanković, Nevena, Mladenović, Milan, Matić, Sanja, Stanić, Snežana, Stanković, Vesna, Mihailović, Mirjana, Mihailović, Vladimir, Katanić, Jelena, Boroja, Tatjana, Vuković, Nenad, Sukdolak, Slobodan, "Serum albumin binding analysis and toxicological screening of novel
 chroman-2,4-diones as oral anticoagulants" in Chemico-Biological Interactions, 227 (2015):18-31,
https://doi.org/10.1016/j.cbi.2014.12.005 . .

TY  - JOUR
AU  - Mihailović, Vladimir
AU  - Mišić, Danijela
AU  - Matić, Sanja
AU  - Mihailović, Mirjana
AU  - Stanić, Snežana
AU  - Vrvić, Miroslav M.
AU  - Katanić, Jelena
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Stanković, Milan S.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2350
AB  - The purpose of this study was to evaluate the antioxidant potential of
   methanol extracts of Gentiana crudata L. aerial parts and roots, as well
   as the stability of the phenolic compounds and antioxidant capacity of
   extracts during heating, at different pHs and after an in vitro
   digestion procedure. Also, their genotoxicity and antigenotoxicity
   against carbon tetrachloride in the liver of albino Wistar rats using
   the comet assay were evaluated. Three secoiridoid glycosides
   (swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
   (orientin, vitexin and two isovitexin-glucosides) were identified as the
   major constituents in aerial parts and roots of G. cruciata, using
   UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
   showed that aerial parts displayed higher antioxidant activity compared
   to the roots, which could be related to higher phenolics content,
   especially flavonoids. In general, extracts showed pH and thermal
   stability, while duodenal condition had more influence on total phenolic
   condition and antioxidant activity of extracts. Both extracts showed a
   protective effect against CCl4 in comet assays. The roots extract showed
   no genotoxic activity, while aerial parts extract showed slight
   genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
   All rights reserved.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities
VL  - 73
DO  - 10.1016/j.indcrop.2015.04.013
SP  - 49
EP  - 62
ER  - 

@article{
author = "Mihailović, Vladimir and Mišić, Danijela and Matić, Sanja and Mihailović, Mirjana and Stanić, Snežana and Vrvić, Miroslav M. and Katanić, Jelena and Mladenović, Milan and Stanković, Nevena and Boroja, Tatjana and Stanković, Milan S.",
year = "2015",
abstract = "The purpose of this study was to evaluate the antioxidant potential of
   methanol extracts of Gentiana crudata L. aerial parts and roots, as well
   as the stability of the phenolic compounds and antioxidant capacity of
   extracts during heating, at different pHs and after an in vitro
   digestion procedure. Also, their genotoxicity and antigenotoxicity
   against carbon tetrachloride in the liver of albino Wistar rats using
   the comet assay were evaluated. Three secoiridoid glycosides
   (swertiamarin, gentiopicrin, and sweroside) and four phenolic compounds
   (orientin, vitexin and two isovitexin-glucosides) were identified as the
   major constituents in aerial parts and roots of G. cruciata, using
   UHPLC-DAD/+/- HESI-MS/MS analysis. The results of antioxidant assays
   showed that aerial parts displayed higher antioxidant activity compared
   to the roots, which could be related to higher phenolics content,
   especially flavonoids. In general, extracts showed pH and thermal
   stability, while duodenal condition had more influence on total phenolic
   condition and antioxidant activity of extracts. Both extracts showed a
   protective effect against CCl4 in comet assays. The roots extract showed
   no genotoxic activity, while aerial parts extract showed slight
   genotoxicity at concentrations of 400 mg/kg b.w. (C) 2015 Elsevier B.V.
   All rights reserved.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities",
volume = "73",
doi = "10.1016/j.indcrop.2015.04.013",
pages = "49-62"
}

Mihailović, V., Mišić, D., Matić, S., Mihailović, M., Stanić, S., Vrvić, M. M., Katanić, J., Mladenović, M., Stanković, N., Boroja, T.,& Stanković, M. S.. (2015). Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities. in Industrial Crops and Products
Elsevier., 73, 49-62.
https://doi.org/10.1016/j.indcrop.2015.04.013

Mihailović V, Mišić D, Matić S, Mihailović M, Stanić S, Vrvić MM, Katanić J, Mladenović M, Stanković N, Boroja T, Stanković MS. Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities. in Industrial Crops and Products. 2015;73:49-62.
doi:10.1016/j.indcrop.2015.04.013 .

Mihailović, Vladimir, Mišić, Danijela, Matić, Sanja, Mihailović, Mirjana, Stanić, Snežana, Vrvić, Miroslav M., Katanić, Jelena, Mladenović, Milan, Stanković, Nevena, Boroja, Tatjana, Stanković, Milan S., "Comparative phytochemical analysis of Gentiana cruciata L. roots and
 aerial parts, and their biological activities" in Industrial Crops and Products, 73 (2015):49-62,
https://doi.org/10.1016/j.indcrop.2015.04.013 . .

TY  - JOUR
AU  - Katanić, Jelena
AU  - Mihailović, Vladimir
AU  - Matić, Sanja
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Mladenović, Milan
AU  - Stanić, Snezana
AU  - Kreft, Samo
AU  - Mihailović, Mirjana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2352
UR  - http://www.sciencedirect.com/science/article/pii/S175646461500362X
AB  - The effects of the methanolic extracts of Filipendula hexapetala Gilib.
   aerial parts (FHA) and roots (FHR) against cisplatin induced kidney and
   liver injuries in rats were investigated as well as determination of
   genotoxicity and antigenotoxicity of the extracts. Treatment with FHA
   and FHR significantly decreased levels of urea, uric acid, serum
   transaminases, alkaline phosphatase and gamma-glutamyl transferase, and
   increased the content of total protein. In addition, treatment with the
   extracts significantly attenuated the cisplatin-induced oxidative stress
   in kidney and liver tissues by increasing catalase and superoxide
   dismutase activities and the content of reduced glutathione and
   decreasing the content of thiobarbituric acid reactive substances
   (TSARS). The histopathological studies confirmed the protective effects
   of the extracts against cisplatin-induced kidney and liver injuries. The
   extracts ameliorated cisplatin-induced genotoxicity. These results
   suggest that F. hexapetala extracts are effective nephro- and
   hepatoprotective agents, with potential to reduce oxidative stress and
   ameliorate cisplatin-induced nephro- and hepatotoxicity.
T2  - Journal of Functional Foods
T1  - The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin
IS  - Part A
VL  - 18
DO  - 10.1016/j.jff.2015.07.004
SP  - 198
EP  - 212
ER  - 

@article{
author = "Katanić, Jelena and Mihailović, Vladimir and Matić, Sanja and Stanković, Vesna and Stanković, Nevena and Boroja, Tatjana and Mladenović, Milan and Stanić, Snezana and Kreft, Samo and Mihailović, Mirjana",
year = "2015",
abstract = "The effects of the methanolic extracts of Filipendula hexapetala Gilib.
   aerial parts (FHA) and roots (FHR) against cisplatin induced kidney and
   liver injuries in rats were investigated as well as determination of
   genotoxicity and antigenotoxicity of the extracts. Treatment with FHA
   and FHR significantly decreased levels of urea, uric acid, serum
   transaminases, alkaline phosphatase and gamma-glutamyl transferase, and
   increased the content of total protein. In addition, treatment with the
   extracts significantly attenuated the cisplatin-induced oxidative stress
   in kidney and liver tissues by increasing catalase and superoxide
   dismutase activities and the content of reduced glutathione and
   decreasing the content of thiobarbituric acid reactive substances
   (TSARS). The histopathological studies confirmed the protective effects
   of the extracts against cisplatin-induced kidney and liver injuries. The
   extracts ameliorated cisplatin-induced genotoxicity. These results
   suggest that F. hexapetala extracts are effective nephro- and
   hepatoprotective agents, with potential to reduce oxidative stress and
   ameliorate cisplatin-induced nephro- and hepatotoxicity.",
journal = "Journal of Functional Foods",
title = "The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin",
number = "Part A",
volume = "18",
doi = "10.1016/j.jff.2015.07.004",
pages = "198-212"
}

Katanić, J., Mihailović, V., Matić, S., Stanković, V., Stanković, N., Boroja, T., Mladenović, M., Stanić, S., Kreft, S.,& Mihailović, M.. (2015). The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin. in Journal of Functional Foods, 18(Part A), 198-212.
https://doi.org/10.1016/j.jff.2015.07.004

Katanić J, Mihailović V, Matić S, Stanković V, Stanković N, Boroja T, Mladenović M, Stanić S, Kreft S, Mihailović M. The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin. in Journal of Functional Foods. 2015;18(Part A):198-212.
doi:10.1016/j.jff.2015.07.004 .

Katanić, Jelena, Mihailović, Vladimir, Matić, Sanja, Stanković, Vesna, Stanković, Nevena, Boroja, Tatjana, Mladenović, Milan, Stanić, Snezana, Kreft, Samo, Mihailović, Mirjana, "The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin" in Journal of Functional Foods, 18, no. Part A (2015):198-212,
https://doi.org/10.1016/j.jff.2015.07.004 . .