TY  - JOUR
AU  - Saksida, Tamara
AU  - Miljković, Đorđe
AU  - Dekanski, Dragana P.
AU  - Stojanović, Ivana D.
AU  - Stošić-Grujičić, Stanislava
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/277
AB  - We have recently demonstrated the beneficial effects of dry olive leaf extract (DOLE) in two preclinical models of type 1 diabetes. Here we analyze the potential mechanisms underlying diabetes amelioration at the level of lymph node drainage. Treatment of C57BL/6 mice with DOLE during induction of diabetes with multiple low doses of streptozotocin (MLD-SZ) modulated cytokine expression and production in pancreatic lymph node cells, thereby changing the balance between potentially pathogenic and down-regulating cytokines. These results support the immunoregulatory potential of DOLE which takes place at the level of lymph node drainage and preserves the target tissue from autoimmune attack.
T2  - Archives of Biological Sciences
T1  - Dry olive leaf extract (DOLE) down-regulates the progression of experimental immune-mediated diabetes by modulation of cytokine profile in the draining lymph nodes
IS  - 2
VL  - 63
DO  - 10.2298/ABS1102289S
SP  - 289
EP  - 297
ER  - 

@article{
author = "Saksida, Tamara and Miljković, Đorđe and Dekanski, Dragana P. and Stojanović, Ivana D. and Stošić-Grujičić, Stanislava",
year = "2011, 2011",
abstract = "We have recently demonstrated the beneficial effects of dry olive leaf extract (DOLE) in two preclinical models of type 1 diabetes. Here we analyze the potential mechanisms underlying diabetes amelioration at the level of lymph node drainage. Treatment of C57BL/6 mice with DOLE during induction of diabetes with multiple low doses of streptozotocin (MLD-SZ) modulated cytokine expression and production in pancreatic lymph node cells, thereby changing the balance between potentially pathogenic and down-regulating cytokines. These results support the immunoregulatory potential of DOLE which takes place at the level of lymph node drainage and preserves the target tissue from autoimmune attack.",
journal = "Archives of Biological Sciences",
title = "Dry olive leaf extract (DOLE) down-regulates the progression of experimental immune-mediated diabetes by modulation of cytokine profile in the draining lymph nodes",
number = "2",
volume = "63",
doi = "10.2298/ABS1102289S",
pages = "289-297"
}

Saksida, T., Miljković, Đ., Dekanski, D. P., Stojanović, I. D.,& Stošić-Grujičić, S.. (2011). Dry olive leaf extract (DOLE) down-regulates the progression of experimental immune-mediated diabetes by modulation of cytokine profile in the draining lymph nodes. in Archives of Biological Sciences, 63(2), 289-297.
https://doi.org/10.2298/ABS1102289S

Saksida T, Miljković Đ, Dekanski DP, Stojanović ID, Stošić-Grujičić S. Dry olive leaf extract (DOLE) down-regulates the progression of experimental immune-mediated diabetes by modulation of cytokine profile in the draining lymph nodes. in Archives of Biological Sciences. 2011;63(2):289-297.
doi:10.2298/ABS1102289S .

Saksida, Tamara, Miljković, Đorđe, Dekanski, Dragana P., Stojanović, Ivana D., Stošić-Grujičić, Stanislava, "Dry olive leaf extract (DOLE) down-regulates the progression of experimental immune-mediated diabetes by modulation of cytokine profile in the draining lymph nodes" in Archives of Biological Sciences, 63, no. 2 (2011):289-297,
https://doi.org/10.2298/ABS1102289S . .

TY  - JOUR
AU  - Mijatović, Sanja
AU  - Timotijević, Gordana S
AU  - Miljković, Đorđe
AU  - Radović, Julijana M
AU  - Maksimović-Ivanić, Danijela
AU  - Dekanski, Dragana P.
AU  - Stošić-Grujičić, Stanislava
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1297
AB  - Various constituents of the olive tree (Olea europaea) have been traditionally used in the treatment of infection, inflammation, prevention of chronic diseases, cardiovascular disorders and cancer. The anticancer potential of dry olive leaf extract (DOLE) represents the net effect of multilevel interactions between different biologically active compounds from the extract, cancer cells and conventional therapy. In this context, it was of primary interest to evaluate the influence of DOLE on progression of the highly malignant, immuno-and chemoresistant type of skin cancer-melanoma. DOLE significantly inhibited proliferation and subsequently restricted clonogenicity of the B16 mouse melanoma cell line in vitro. Moreover, late phase tumor treatment with DOLE significantly reduced tumor volume in a syngeneic strain of mice. DOLE-treated B16 cells were blocked in the G(0)/G(1) phase of the cell cycle, underwent early apoptosis and died by late necrosis. At the molecular level, the dying process started as caspase dependent, but finalized as caspase independent. In concordance, overexpression of antiapoptotic members of the Bcl-2 family, Bcl-2 and Bcl-XL, and diminished expression of their natural antagonists, Bim and p53, were observed. Despite molecular suppression of the proapoptotic process, DOLE successfully promoted cell death mainly through disruption of cell membrane integrity and late caspase-independent fragmentation of genetic material. Taken together, the results of this study indicate that DOLE possesses strong antimelanoma potential. When DOLE was applied in combination with different chemotherapeutics, various outcomes, including synergy and antagonism, were observed. This requires caution in the use of the extract as a supplementary antitumor therapeutic.
T2  - International Journal of Cancer
T1  - Multiple antimelanoma potential of dry olive leaf extract
IS  - 8
VL  - 128
EP  - 1965
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1297
ER  - 

@article{
author = "Mijatović, Sanja and Timotijević, Gordana S and Miljković, Đorđe and Radović, Julijana M and Maksimović-Ivanić, Danijela and Dekanski, Dragana P. and Stošić-Grujičić, Stanislava",
year = "2011",
abstract = "Various constituents of the olive tree (Olea europaea) have been traditionally used in the treatment of infection, inflammation, prevention of chronic diseases, cardiovascular disorders and cancer. The anticancer potential of dry olive leaf extract (DOLE) represents the net effect of multilevel interactions between different biologically active compounds from the extract, cancer cells and conventional therapy. In this context, it was of primary interest to evaluate the influence of DOLE on progression of the highly malignant, immuno-and chemoresistant type of skin cancer-melanoma. DOLE significantly inhibited proliferation and subsequently restricted clonogenicity of the B16 mouse melanoma cell line in vitro. Moreover, late phase tumor treatment with DOLE significantly reduced tumor volume in a syngeneic strain of mice. DOLE-treated B16 cells were blocked in the G(0)/G(1) phase of the cell cycle, underwent early apoptosis and died by late necrosis. At the molecular level, the dying process started as caspase dependent, but finalized as caspase independent. In concordance, overexpression of antiapoptotic members of the Bcl-2 family, Bcl-2 and Bcl-XL, and diminished expression of their natural antagonists, Bim and p53, were observed. Despite molecular suppression of the proapoptotic process, DOLE successfully promoted cell death mainly through disruption of cell membrane integrity and late caspase-independent fragmentation of genetic material. Taken together, the results of this study indicate that DOLE possesses strong antimelanoma potential. When DOLE was applied in combination with different chemotherapeutics, various outcomes, including synergy and antagonism, were observed. This requires caution in the use of the extract as a supplementary antitumor therapeutic.",
journal = "International Journal of Cancer",
title = "Multiple antimelanoma potential of dry olive leaf extract",
number = "8",
volume = "128",
pages = "1965",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1297"
}

Mijatović, S., Timotijević, G. S., Miljković, Đ., Radović, J. M., Maksimović-Ivanić, D., Dekanski, D. P.,& Stošić-Grujičić, S.. (2011). Multiple antimelanoma potential of dry olive leaf extract. in International Journal of Cancer, 128(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1297

Mijatović S, Timotijević GS, Miljković Đ, Radović JM, Maksimović-Ivanić D, Dekanski DP, Stošić-Grujičić S. Multiple antimelanoma potential of dry olive leaf extract. in International Journal of Cancer. 2011;128(8):null-1965.
https://hdl.handle.net/21.15107/rcub_ibiss_1297 .

Mijatović, Sanja, Timotijević, Gordana S, Miljković, Đorđe, Radović, Julijana M, Maksimović-Ivanić, Danijela, Dekanski, Dragana P., Stošić-Grujičić, Stanislava, "Multiple antimelanoma potential of dry olive leaf extract" in International Journal of Cancer, 128, no. 8 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1297 .

TY  - JOUR
AU  - Cvjetičanin, Tamara
AU  - Miljković, Đorđe
AU  - Stojanović, Ivana D.
AU  - Stošić-Grujičić, Stanislava
AU  - Dekanski, Dragana P.
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1376
AB  - The health-promoting effects of various constituents of the olive tree (Olea europaea) are mainly associated with hypoglycaemic and insulin-sensitising activities and have been widely demonstrated in the metabolic syndrome and type 2 diabetes. However, their biological activity in autoimmune type I diabetes (TID) is poorly characterised. Therefore, the influence of O. europaea-derived components present in dry olive leaf extract (DOLE) was examined in two established preclinical models of human TID, which differ in some aspects of diabetogenesis: multiple low-dose streptozotocin-induced diabetes in susceptible C57BL/6 and CBA/H mouse strains; cyclophosphamide-accelerated diabetes in non-obese diabetic mice. In both T I D models, in vivo administration of DOLE significantly reduced clinical signs of diabetes (hyperglycaemia and body weight loss) and led to complete suppression of histopathological changes in pancreatic islets. In line with these, insulin expression and release were restored in DOLE-treated mice. Interestingly, inducible NO synthase expression and NO production were significantly elevated in peripheral tissues but were down-regulated within the local environment of the endocrine pancreas. This interference was reflected in NO-mediated suppression of T lymphocyte proliferation and lower production of the proinflammatory cytokines interferon-gamma, IL-17 and TNF-alpha in the spleen. with subsequent blockade of beta-cell destruction. The results suggest that DOLE interferes with development of autoimmune diabetes by down-regulating production of proinflammatory and cytotoxic mediators. Therefore, the potential use of a DOLE-enriched diet for prophylaxis/treatment of human T I D. and possibly other autoimmune diseases, is worthy of further investigation.
T2  - British Journal of Nutrition
T1  - Dried leaf extract of Olea europaea ameliorates islet-directed autoimmunity in mice
IS  - 10
VL  - 103
EP  - 1424
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1376
ER  - 

@article{
author = "Cvjetičanin, Tamara and Miljković, Đorđe and Stojanović, Ivana D. and Stošić-Grujičić, Stanislava and Dekanski, Dragana P.",
year = "2010",
abstract = "The health-promoting effects of various constituents of the olive tree (Olea europaea) are mainly associated with hypoglycaemic and insulin-sensitising activities and have been widely demonstrated in the metabolic syndrome and type 2 diabetes. However, their biological activity in autoimmune type I diabetes (TID) is poorly characterised. Therefore, the influence of O. europaea-derived components present in dry olive leaf extract (DOLE) was examined in two established preclinical models of human TID, which differ in some aspects of diabetogenesis: multiple low-dose streptozotocin-induced diabetes in susceptible C57BL/6 and CBA/H mouse strains; cyclophosphamide-accelerated diabetes in non-obese diabetic mice. In both T I D models, in vivo administration of DOLE significantly reduced clinical signs of diabetes (hyperglycaemia and body weight loss) and led to complete suppression of histopathological changes in pancreatic islets. In line with these, insulin expression and release were restored in DOLE-treated mice. Interestingly, inducible NO synthase expression and NO production were significantly elevated in peripheral tissues but were down-regulated within the local environment of the endocrine pancreas. This interference was reflected in NO-mediated suppression of T lymphocyte proliferation and lower production of the proinflammatory cytokines interferon-gamma, IL-17 and TNF-alpha in the spleen. with subsequent blockade of beta-cell destruction. The results suggest that DOLE interferes with development of autoimmune diabetes by down-regulating production of proinflammatory and cytotoxic mediators. Therefore, the potential use of a DOLE-enriched diet for prophylaxis/treatment of human T I D. and possibly other autoimmune diseases, is worthy of further investigation.",
journal = "British Journal of Nutrition",
title = "Dried leaf extract of Olea europaea ameliorates islet-directed autoimmunity in mice",
number = "10",
volume = "103",
pages = "1424",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1376"
}

Cvjetičanin, T., Miljković, Đ., Stojanović, I. D., Stošić-Grujičić, S.,& Dekanski, D. P.. (2010). Dried leaf extract of Olea europaea ameliorates islet-directed autoimmunity in mice. in British Journal of Nutrition, 103(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1376

Cvjetičanin T, Miljković Đ, Stojanović ID, Stošić-Grujičić S, Dekanski DP. Dried leaf extract of Olea europaea ameliorates islet-directed autoimmunity in mice. in British Journal of Nutrition. 2010;103(10):null-1424.
https://hdl.handle.net/21.15107/rcub_ibiss_1376 .

Cvjetičanin, Tamara, Miljković, Đorđe, Stojanović, Ivana D., Stošić-Grujičić, Stanislava, Dekanski, Dragana P., "Dried leaf extract of Olea europaea ameliorates islet-directed autoimmunity in mice" in British Journal of Nutrition, 103, no. 10 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1376 .

TY  - JOUR
AU  - Miljković, Đorđe
AU  - Dekanski, Dragana P.
AU  - Miljković, Zeljka
AU  - Momčilović, Miljana
AU  - Mostarica-Stojković, Marija B
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1447
AB  - Background & aims: Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Mediterranean diet, rich in olive products is associated with lower incidence of multiple sclerosis in South European population. Therefore, the influence of dry olive leaf extract (DOLE) on EAE course was investigated. Methods: Spinal cord homogenate and complete Freund's adjuvant were used for the induction of EAE in Dark Agouti rats. DOLE was applied intragastrically once per day, starting from the day of the immunization. Real time PCR and ELISA were used for the determination of IFN-gamma and IL-17 gene expression and production, respectively. Results: DOLE reduced various parameters of EAE severity in DA rats, including cumulative disease index, maximal clinical score and disease duration. Also, DOLE decreased cellularity of the draining lymph nodes and production of IFN-gamma and IL-17 by the cells infiltrating spinal cord of EAE rats. Conclusions: The results presented in this paper strongly suggest that DOLE-enriched diet has a beneficial effect in EAE in rats. Further studies in humans are required in order to investigate if DOLE could be a useful supplementary dietetic for the patients suffering from multiple sclerosis and other neuroinflammatory disorders. (C) 2009 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.
T2  - Clinical Nutrition
T1  - Dry olive leaf extract ameliorates experimental autoimmune encephalomyelitis
IS  - 3
VL  - 28
EP  - 350
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1447
ER  - 

@article{
author = "Miljković, Đorđe and Dekanski, Dragana P. and Miljković, Zeljka and Momčilović, Miljana and Mostarica-Stojković, Marija B",
year = "2009",
abstract = "Background & aims: Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Mediterranean diet, rich in olive products is associated with lower incidence of multiple sclerosis in South European population. Therefore, the influence of dry olive leaf extract (DOLE) on EAE course was investigated. Methods: Spinal cord homogenate and complete Freund's adjuvant were used for the induction of EAE in Dark Agouti rats. DOLE was applied intragastrically once per day, starting from the day of the immunization. Real time PCR and ELISA were used for the determination of IFN-gamma and IL-17 gene expression and production, respectively. Results: DOLE reduced various parameters of EAE severity in DA rats, including cumulative disease index, maximal clinical score and disease duration. Also, DOLE decreased cellularity of the draining lymph nodes and production of IFN-gamma and IL-17 by the cells infiltrating spinal cord of EAE rats. Conclusions: The results presented in this paper strongly suggest that DOLE-enriched diet has a beneficial effect in EAE in rats. Further studies in humans are required in order to investigate if DOLE could be a useful supplementary dietetic for the patients suffering from multiple sclerosis and other neuroinflammatory disorders. (C) 2009 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.",
journal = "Clinical Nutrition",
title = "Dry olive leaf extract ameliorates experimental autoimmune encephalomyelitis",
number = "3",
volume = "28",
pages = "350",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1447"
}

Miljković, Đ., Dekanski, D. P., Miljković, Z., Momčilović, M.,& Mostarica-Stojković, M. B.. (2009). Dry olive leaf extract ameliorates experimental autoimmune encephalomyelitis. in Clinical Nutrition, 28(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1447

Miljković Đ, Dekanski DP, Miljković Z, Momčilović M, Mostarica-Stojković MB. Dry olive leaf extract ameliorates experimental autoimmune encephalomyelitis. in Clinical Nutrition. 2009;28(3):null-350.
https://hdl.handle.net/21.15107/rcub_ibiss_1447 .

Miljković, Đorđe, Dekanski, Dragana P., Miljković, Zeljka, Momčilović, Miljana, Mostarica-Stojković, Marija B, "Dry olive leaf extract ameliorates experimental autoimmune encephalomyelitis" in Clinical Nutrition, 28, no. 3 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1447 .

TY  - CONF
AU  - Mijatović, Sanja
AU  - Radović, Julijana M
AU  - Timotijević, Gordana S
AU  - Mojić, Marija
AU  - Miljković, Đorđe
AU  - Dekanski, Dragana P.
AU  - Stošić-Grujičić, Stanislava
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1443
C3  - Planta Medica
T1  - Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway
IS  - 9
VL  - 75
EP  - 903
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1443
ER  - 

@conference{
author = "Mijatović, Sanja and Radović, Julijana M and Timotijević, Gordana S and Mojić, Marija and Miljković, Đorđe and Dekanski, Dragana P. and Stošić-Grujičić, Stanislava",
year = "2009",
journal = "Planta Medica",
title = "Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway",
number = "9",
volume = "75",
pages = "903",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1443"
}

Mijatović, S., Radović, J. M., Timotijević, G. S., Mojić, M., Miljković, Đ., Dekanski, D. P.,& Stošić-Grujičić, S.. (2009). Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway. in Planta Medica, 75(9).
https://hdl.handle.net/21.15107/rcub_ibiss_1443

Mijatović S, Radović JM, Timotijević GS, Mojić M, Miljković Đ, Dekanski DP, Stošić-Grujičić S. Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway. in Planta Medica. 2009;75(9):null-903.
https://hdl.handle.net/21.15107/rcub_ibiss_1443 .

Mijatović, Sanja, Radović, Julijana M, Timotijević, Gordana S, Mojić, Marija, Miljković, Đorđe, Dekanski, Dragana P., Stošić-Grujičić, Stanislava, "Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway" in Planta Medica, 75, no. 9 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1443 .