TY  - JOUR
AU  - Pergal, Marija V.
AU  - Kodranov, Igor D.
AU  - Pergal, Miodrag M.
AU  - Gašić, Uroš
AU  - Stanković, Dalibor M.
AU  - Petković, Branka B.
AU  - Manojlović, Dragan D.
PY  - 2020
UR  - https://doi.org/10.1007/s11270-020-04800-x
UR  - https://radar.ibiss.bg.ac.rs/123456789/3852
AB  - The aim of this study was to investigate, analyze, and compare applied techniques suitable for achieving efficient removal of organophosphorus pesticides (OPPs) (malathion and fenitrothion) from aqueous solutions and analyze the degradation products and processes. Pesticide degradation efficiency (%) was monitored by high-performance liquid chromatography (HPLC) equipped with a photodiode array detector (DAD), while mineralization degree was determined by total organic carbon analysis (TOC). Daphnia magna was used for screening the environmental safety aspects of the degradation methods, i.e., for assessing the toxicity of solutions obtained after degradation. Additionally, a surface river water was utilized to examine the likely influence of organic matter on the pesticides’ degradation. Pesticide degradation products were identified using gas chromatography with a triple quadrupole mass detector (GC-MS/MS) as well as ultrahigh-performance liquid chromatography coupled with a linear ion trap, Orbitrap mass spectrometer (UHPLC-LTQ Orbitrap MS), and a simple pesticide degradation mechanism is proposed. Removal of pesticides from water using chlorine dioxide was successful, resulting in high degradation efficiency (98% for malathion and 81% for fenitrothion). Partial mineralization was achieved, and Daphnia magna mortality decreased in the waters containing degradation products (compared with the parent pesticides), indicating that the solutions formed were less toxic than the parent pesticides. Lower degradation rates (80% for malathion and 72% for fenitrothion) in Sava River water were measured, indicating the influence of the organic matter contained in this naturally occurring surface water. The results prove that chlorine dioxide could be used as an agent for successful removal of these OPPs from water.
PB  - Springer
T2  - Water, Air, and Soil Pollution
T1  - Degradation Products, Mineralization, and Toxicity Assessment of Pesticides Malathion and Fenitrothion
IS  - 8
VL  - 231
DO  - 10.1007/s11270-020-04800-x
SP  - 433
ER  - 

@article{
author = "Pergal, Marija V. and Kodranov, Igor D. and Pergal, Miodrag M. and Gašić, Uroš and Stanković, Dalibor M. and Petković, Branka B. and Manojlović, Dragan D.",
year = "2020",
abstract = "The aim of this study was to investigate, analyze, and compare applied techniques suitable for achieving efficient removal of organophosphorus pesticides (OPPs) (malathion and fenitrothion) from aqueous solutions and analyze the degradation products and processes. Pesticide degradation efficiency (%) was monitored by high-performance liquid chromatography (HPLC) equipped with a photodiode array detector (DAD), while mineralization degree was determined by total organic carbon analysis (TOC). Daphnia magna was used for screening the environmental safety aspects of the degradation methods, i.e., for assessing the toxicity of solutions obtained after degradation. Additionally, a surface river water was utilized to examine the likely influence of organic matter on the pesticides’ degradation. Pesticide degradation products were identified using gas chromatography with a triple quadrupole mass detector (GC-MS/MS) as well as ultrahigh-performance liquid chromatography coupled with a linear ion trap, Orbitrap mass spectrometer (UHPLC-LTQ Orbitrap MS), and a simple pesticide degradation mechanism is proposed. Removal of pesticides from water using chlorine dioxide was successful, resulting in high degradation efficiency (98% for malathion and 81% for fenitrothion). Partial mineralization was achieved, and Daphnia magna mortality decreased in the waters containing degradation products (compared with the parent pesticides), indicating that the solutions formed were less toxic than the parent pesticides. Lower degradation rates (80% for malathion and 72% for fenitrothion) in Sava River water were measured, indicating the influence of the organic matter contained in this naturally occurring surface water. The results prove that chlorine dioxide could be used as an agent for successful removal of these OPPs from water.",
publisher = "Springer",
journal = "Water, Air, and Soil Pollution",
title = "Degradation Products, Mineralization, and Toxicity Assessment of Pesticides Malathion and Fenitrothion",
number = "8",
volume = "231",
doi = "10.1007/s11270-020-04800-x",
pages = "433"
}

Pergal, M. V., Kodranov, I. D., Pergal, M. M., Gašić, U., Stanković, D. M., Petković, B. B.,& Manojlović, D. D.. (2020). Degradation Products, Mineralization, and Toxicity Assessment of Pesticides Malathion and Fenitrothion. in Water, Air, and Soil Pollution
Springer., 231(8), 433.
https://doi.org/10.1007/s11270-020-04800-x

Pergal MV, Kodranov ID, Pergal MM, Gašić U, Stanković DM, Petković BB, Manojlović DD. Degradation Products, Mineralization, and Toxicity Assessment of Pesticides Malathion and Fenitrothion. in Water, Air, and Soil Pollution. 2020;231(8):433.
doi:10.1007/s11270-020-04800-x .

Pergal, Marija V., Kodranov, Igor D., Pergal, Miodrag M., Gašić, Uroš, Stanković, Dalibor M., Petković, Branka B., Manojlović, Dragan D., "Degradation Products, Mineralization, and Toxicity Assessment of Pesticides Malathion and Fenitrothion" in Water, Air, and Soil Pollution, 231, no. 8 (2020):433,
https://doi.org/10.1007/s11270-020-04800-x . .

TY  - JOUR
AU  - Korać Jačić, Jelena
AU  - Nikolić, Ljiljana
AU  - Stanković, Dalibor M.
AU  - Opačić, Miloš
AU  - Dimitrijević, Milena
AU  - Savić, Danijela
AU  - Grgurić Šipka, Sanja
AU  - Spasojević, Ivan
AU  - Bogdanović Pristov, Jelena
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3597
AB  - Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.
T2  - Free Radical Biology and Medicine
T1  - Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.
VL  - 148
DO  - 10.1016/j.freeradbiomed.2020.01.001
SP  - 123
EP  - 127
ER  - 

@article{
author = "Korać Jačić, Jelena and Nikolić, Ljiljana and Stanković, Dalibor M. and Opačić, Miloš and Dimitrijević, Milena and Savić, Danijela and Grgurić Šipka, Sanja and Spasojević, Ivan and Bogdanović Pristov, Jelena",
year = "2020",
abstract = "Upon release in response to stress, epinephrine (Epi) may interact with labile iron pool in human plasma with potentially important (patho)physiological consequences. We have shown that Epi and Fe3+ build stable 1:1 high-spin bidentate complex at physiological pH, and that Epi does not undergo degradation in the presence of iron. However, the interactions of Epi with the more soluble Fe2+, and the impact of iron on biological activity of Epi are still not known. Herein we showed that Epi and Fe2+ build colorless complex which is stable under anaerobic conditions. In the presence of O2, Epi promoted the oxidation of Fe2+ and the formation of Epi-Fe3+ complex. Cyclic voltammetry showed that mid-point potential of Epi-Fe2+ complex is very low (-582 mV vs. standard hydrogen electrode), which explains catalyzed oxidation of Fe2+. Next, we examined the impact of iron binding on biological performance of Epi using patch clamping in cell culture with constitutive expression of adrenergic receptors. Epi alone evoked an increase of outward currents, whereas Epi in the complex with Fe3+ did not. This implies that the binding of Epi to adrenergic receptors and their activation is prevented by the formation of complex with iron. Pro-oxidative activity of Epi-Fe2+ complex may represent a link between chronic stress and cardiovascular problems. On the other hand, labile iron could serve as a modulator of biological activity of ligands. Such interactions may be important in human pathologies that are related to iron overload or deficiency.",
journal = "Free Radical Biology and Medicine",
title = "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.",
volume = "148",
doi = "10.1016/j.freeradbiomed.2020.01.001",
pages = "123-127"
}

Korać Jačić, J., Nikolić, L., Stanković, D. M., Opačić, M., Dimitrijević, M., Savić, D., Grgurić Šipka, S., Spasojević, I.,& Bogdanović Pristov, J.. (2020). Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.. in Free Radical Biology and Medicine, 148, 123-127.
https://doi.org/10.1016/j.freeradbiomed.2020.01.001

Korać Jačić J, Nikolić L, Stanković DM, Opačić M, Dimitrijević M, Savić D, Grgurić Šipka S, Spasojević I, Bogdanović Pristov J. Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors.. in Free Radical Biology and Medicine. 2020;148:123-127.
doi:10.1016/j.freeradbiomed.2020.01.001 .

Korać Jačić, Jelena, Nikolić, Ljiljana, Stanković, Dalibor M., Opačić, Miloš, Dimitrijević, Milena, Savić, Danijela, Grgurić Šipka, Sanja, Spasojević, Ivan, Bogdanović Pristov, Jelena, "Ferrous iron binding to epinephrine promotes the oxidation of iron and impedes activation of adrenergic receptors." in Free Radical Biology and Medicine, 148 (2020):123-127,
https://doi.org/10.1016/j.freeradbiomed.2020.01.001 . .

TY  - CONF
AU  - Korać, Jelena
AU  - Stanković, Dalibor
AU  - Bogdanović Pristov, Jelena
AU  - Nikolić, Ljiljana
AU  - Spasojević, Ivan
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5517
AB  - Epinephrine ((R)-4-(1-hydroxy-2-(methylamino)ethyl)-benzene-1,2-diol (Epi) is
catecholamine that is released by the sympathetic nervous system and adrenal medulla. It
is a physiologically important molecule that acts as a hormone, neurotransmitter, and
medication with a broad range of effects 1-3 . Coordinate and redox interaction of Epi with
iron affects the interactions with other molecules and its biological effects 4
. In this study,
we reported details of redox interactions of Epi with Fe 2+ at pH 7.4, which correspond to
the pH value of human plasma Epi and Fe 2+ form a complex that acts as a strong reducing
agent. Cyclic voltammetry showed that the positions of E pa and E pc potentials were at
approximately -480 and -1100 mV. This implies that Epi and Fe 2+ build a complex with
unique redox properties. E1/2 was significantly lower compared to E0' for O 2 /O 2•- (-350
mV). It is important to point out this because superoxide radical anion is produced via
spontaneous Fe 2+ reaction with O 2. In other words, Epi-Fe 2+ complex should be capable of
reducing transition metals in (patho)physiologicaly relevant complexes that are not
susceptible to reduction by O 2. Our results confirmed that Epi-Fe
2+ is capable of reducing
the S-S group of glutathione disulfide. On the other hand, Epi acted in a catalyst-like
fashion to promote Fe 2+ oxidation by molecular oxygen, and to a facilitated formation of
the Epi–Fe 3+ complexes, at physiological pH. In addition, we examined the effects of
epinepfrine and Epi/Fe3+ system on glioma cells. Epinephrine alone evokes changes in the
membrane currents of glioma cells, but such effects were not observed for the complex
with Fe 3+
. This implies that Epi-Fe 3+ might modulate neural activity of Epi in CNS.
PB  - Belgrade : Faculty of Chemistry : Serbian Biochemical Society
C3  - Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
T1  - Redox interactions of epinephrine with iron at physiological pH
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5517
ER  - 

@conference{
author = "Korać, Jelena and Stanković, Dalibor and Bogdanović Pristov, Jelena and Nikolić, Ljiljana and Spasojević, Ivan",
year = "2018",
abstract = "Epinephrine ((R)-4-(1-hydroxy-2-(methylamino)ethyl)-benzene-1,2-diol (Epi) is
catecholamine that is released by the sympathetic nervous system and adrenal medulla. It
is a physiologically important molecule that acts as a hormone, neurotransmitter, and
medication with a broad range of effects 1-3 . Coordinate and redox interaction of Epi with
iron affects the interactions with other molecules and its biological effects 4
. In this study,
we reported details of redox interactions of Epi with Fe 2+ at pH 7.4, which correspond to
the pH value of human plasma Epi and Fe 2+ form a complex that acts as a strong reducing
agent. Cyclic voltammetry showed that the positions of E pa and E pc potentials were at
approximately -480 and -1100 mV. This implies that Epi and Fe 2+ build a complex with
unique redox properties. E1/2 was significantly lower compared to E0' for O 2 /O 2•- (-350
mV). It is important to point out this because superoxide radical anion is produced via
spontaneous Fe 2+ reaction with O 2. In other words, Epi-Fe 2+ complex should be capable of
reducing transition metals in (patho)physiologicaly relevant complexes that are not
susceptible to reduction by O 2. Our results confirmed that Epi-Fe
2+ is capable of reducing
the S-S group of glutathione disulfide. On the other hand, Epi acted in a catalyst-like
fashion to promote Fe 2+ oxidation by molecular oxygen, and to a facilitated formation of
the Epi–Fe 3+ complexes, at physiological pH. In addition, we examined the effects of
epinepfrine and Epi/Fe3+ system on glioma cells. Epinephrine alone evokes changes in the
membrane currents of glioma cells, but such effects were not observed for the complex
with Fe 3+
. This implies that Epi-Fe 3+ might modulate neural activity of Epi in CNS.",
publisher = "Belgrade : Faculty of Chemistry : Serbian Biochemical Society",
journal = "Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia",
title = "Redox interactions of epinephrine with iron at physiological pH",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5517"
}

Korać, J., Stanković, D., Bogdanović Pristov, J., Nikolić, L.,& Spasojević, I.. (2018). Redox interactions of epinephrine with iron at physiological pH. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia
Belgrade : Faculty of Chemistry : Serbian Biochemical Society., 141.
https://hdl.handle.net/21.15107/rcub_ibiss_5517

Korać J, Stanković D, Bogdanović Pristov J, Nikolić L, Spasojević I. Redox interactions of epinephrine with iron at physiological pH. in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia. 2018;:141.
https://hdl.handle.net/21.15107/rcub_ibiss_5517 .

Korać, Jelena, Stanković, Dalibor, Bogdanović Pristov, Jelena, Nikolić, Ljiljana, Spasojević, Ivan, "Redox interactions of epinephrine with iron at physiological pH" in Proceedings: Serbian Biochemical Society Eigth Conference with international participation: Coordination in Biochemistry and Life; 2018 Nov 16; Novi Sad, Serbia (2018):141,
https://hdl.handle.net/21.15107/rcub_ibiss_5517 .