TY  - JOUR
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6553
AB  - Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.
PB  - Wiley
T2  - CNS Neuroscience and Therapeutics
T1  - The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease
IS  - 3
VL  - 30
DO  - 10.1111/cns.14188
SP  - e14188
ER  - 

@article{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2024",
abstract = "Introduction: In the present study, we assessed the effects of the hyper- harmonized- 
hydroxylated fullerene– water complex (3HFWC) on Alzheimer's disease (AD) neuro
pathological hallmarks in 5XFAD mice, an AD animal model.
 Methods: The 3- week- old 5XFAD mice were exposed to 3HFWC water solution ad li
bitum for 3 months in the presymptomatic phase of pathology. The functional effects 
of the treatment were confirmed through near- infrared spectroscopy (NIRS) analysis 
through machine learning (ML) using artificial neural networks (ANNs) to classify the 
control and 3HFWC- treated brain tissue samples. The effects of 3HFWC treatment 
on amyloid- β (Aβ) accumulation, plaque formation, gliosis, and synaptic plasticity in 
cortical and hippocampal tissue were assessed.
 Results: The 3HFWC treatment significantly decreased the amyloid- β plaque load in 
specific parts of the cerebral cortex. At the same time, 3HFWC treatment did not 
induce the activation of glia (astrocytes and microglia) nor did it negatively affect 
synaptic protein markers (GAP- 43, synaptophysin, and PSD- 95).
 Conclusion: The obtained results point to the potential of 3HFWC, when applied in 
the presymptomatic phase of AD, to interfere with amyloid plaque formation without 
inducing AD- related pathological processes such as neuroinflammation, gliosis, and 
synaptic vulnerability.",
publisher = "Wiley",
journal = "CNS Neuroscience and Therapeutics",
title = "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease",
number = "3",
volume = "30",
doi = "10.1111/cns.14188",
pages = "e14188"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2024). The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics
Wiley., 30(3), e14188.
https://doi.org/10.1111/cns.14188

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease. in CNS Neuroscience and Therapeutics. 2024;30(3):e14188.
doi:10.1111/cns.14188 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "The presymptomatic treatment with 3HFWC nanosubstance  decreased plaque load in 5XFAD mouse model of Alzheimer's  disease" in CNS Neuroscience and Therapeutics, 30, no. 3 (2024):e14188,
https://doi.org/10.1111/cns.14188 . .

TY  - CONF
AU  - Pavković, Željko
AU  - Potrebić, Milica
AU  - Dubljević, Olga
AU  - Srbovan, Maja
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5832
AB  - Adolescence is a period when alcohol abuse is more likely to occur. Use of isoflurane
(a volatile anesthetic) in adolescence has been experimentally shown to alter
responses to ethanol, implying that general exposure to anesthetics during adolescence
may be an environmental risk factor contributing to increased susceptibility to alcohol
use disorder (AUD). Our previous work showed that propofol (intravenous anesthetic)
anesthesia exposure (PAE) in adolescence favors risk-taking and preference for
novel/intense stimuli. However, its influence on alcohol consumption hasn’t been
examined. Therefore, we aimed to investigate whether PAE in adolescence alters
alcohol consumption in alcohol-naïve rats.
Male Wistar rats aged 35 and 50 postnatal days (early-to-mid and mid-to-late
adolescence, respectively) were injected with anesthetic dose of propofol emulsion
(75mg/kg) or saline, separated into individual cages and exposed to two-bottle choice
paradigm (allowing free access to food and two bottles, one containing 2,5% ethanol
in 1% sucrose solution and the other containing tap water). Liquid consumption was
measured for 4 constitutive days. Obtained data showed no preference for alcohol in
all analyzed experimental groups. Moreover, the tendency for alcohol intake was
significantly reduced during first 24h after PAE in early-to-mid adolescent rats.
Overall, our study shows that PAE doesn’t increase voluntary alcohol intake in
peripubertal rats used as a rodent model of adolescence. The obtained findings do not
support the view that exposure to general anesthetics in adolescence is a risk factor for
AUD and suggest that a detailed analysis of the range of anesthetics is needed before
generalizing the data.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Propofol reduces the tendency for alcohol consumption in adolescent rats
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5832
ER  - 

@conference{
author = "Pavković, Željko and Potrebić, Milica and Dubljević, Olga and Srbovan, Maja and Pešić, Vesna",
year = "2023",
abstract = "Adolescence is a period when alcohol abuse is more likely to occur. Use of isoflurane
(a volatile anesthetic) in adolescence has been experimentally shown to alter
responses to ethanol, implying that general exposure to anesthetics during adolescence
may be an environmental risk factor contributing to increased susceptibility to alcohol
use disorder (AUD). Our previous work showed that propofol (intravenous anesthetic)
anesthesia exposure (PAE) in adolescence favors risk-taking and preference for
novel/intense stimuli. However, its influence on alcohol consumption hasn’t been
examined. Therefore, we aimed to investigate whether PAE in adolescence alters
alcohol consumption in alcohol-naïve rats.
Male Wistar rats aged 35 and 50 postnatal days (early-to-mid and mid-to-late
adolescence, respectively) were injected with anesthetic dose of propofol emulsion
(75mg/kg) or saline, separated into individual cages and exposed to two-bottle choice
paradigm (allowing free access to food and two bottles, one containing 2,5% ethanol
in 1% sucrose solution and the other containing tap water). Liquid consumption was
measured for 4 constitutive days. Obtained data showed no preference for alcohol in
all analyzed experimental groups. Moreover, the tendency for alcohol intake was
significantly reduced during first 24h after PAE in early-to-mid adolescent rats.
Overall, our study shows that PAE doesn’t increase voluntary alcohol intake in
peripubertal rats used as a rodent model of adolescence. The obtained findings do not
support the view that exposure to general anesthetics in adolescence is a risk factor for
AUD and suggest that a detailed analysis of the range of anesthetics is needed before
generalizing the data.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Propofol reduces the tendency for alcohol consumption in adolescent rats",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5832"
}

Pavković, Ž., Potrebić, M., Dubljević, O., Srbovan, M.,& Pešić, V.. (2023). Propofol reduces the tendency for alcohol consumption in adolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 70.
https://hdl.handle.net/21.15107/rcub_ibiss_5832

Pavković Ž, Potrebić M, Dubljević O, Srbovan M, Pešić V. Propofol reduces the tendency for alcohol consumption in adolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:70.
https://hdl.handle.net/21.15107/rcub_ibiss_5832 .

Pavković, Željko, Potrebić, Milica, Dubljević, Olga, Srbovan, Maja, Pešić, Vesna, "Propofol reduces the tendency for alcohol consumption in adolescent rats" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):70,
https://hdl.handle.net/21.15107/rcub_ibiss_5832 .

TY  - CONF
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Dubljević, Olga
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5831
AB  - Aim: Microglia are macrophages of the brain with a fundamental role in neuronal
homeostasis. Stress-induced activation of microglia leads to rapid morphological
changes and it is implicated that these alternations could mediate different aspects of
early-life stress psychopathologies. We aimed to examine morphological response of
hippocampal microglia to social isolation using peripubertal rats as a rodent model of
adolescence.
Methods: Male peripubertal Wistar rats were randomly selected for group-housing
(GH; n=3 per cage) or single-housing (SH; n=1 per cage) at postnatal day 29. After
1st and 2nd week of defined housing animals῾ brains were sampled, and used for
microglia iba-1 fluorescent labelling and morphology analysis: one filed per section
per animal (n=4 animals per group) in hippocampal dentate gyrus (HDG). Data for
microglia cell shape (FracLac plugin, ImageJ) and cell ramification (Analyze Skeleton
plugin, ImageJ) were collected from single cells in photomicrograph visualized with a
confocal microscope (Leica Microsystems).
Results: Compared to GH peers, SH rats had lower microglial cell density in the HDG
after the 1st week of defined housing, which was not evident after the 2nd week. Other
parameters of microglia shape and ramification showed no difference between the
groups in neither of the two time points examined.
Conclusions: Obtained results showed that change in the cell density in the HDG was
the only and transient change in microglia morphology due to the social isolation
during adolescence. Further examination of other stress-related brain regions is
needed to define in depth the relationship between the microglia phenotype and earlylife
stress conditions.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Microglial morphological response to the lack of direct social contact in periadolescent rats
SP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5831
ER  - 

@conference{
author = "Potrebić, Milica and Pavković, Željko and Srbovan, Maja and Dubljević, Olga and Pešić, Vesna",
year = "2023",
abstract = "Aim: Microglia are macrophages of the brain with a fundamental role in neuronal
homeostasis. Stress-induced activation of microglia leads to rapid morphological
changes and it is implicated that these alternations could mediate different aspects of
early-life stress psychopathologies. We aimed to examine morphological response of
hippocampal microglia to social isolation using peripubertal rats as a rodent model of
adolescence.
Methods: Male peripubertal Wistar rats were randomly selected for group-housing
(GH; n=3 per cage) or single-housing (SH; n=1 per cage) at postnatal day 29. After
1st and 2nd week of defined housing animals῾ brains were sampled, and used for
microglia iba-1 fluorescent labelling and morphology analysis: one filed per section
per animal (n=4 animals per group) in hippocampal dentate gyrus (HDG). Data for
microglia cell shape (FracLac plugin, ImageJ) and cell ramification (Analyze Skeleton
plugin, ImageJ) were collected from single cells in photomicrograph visualized with a
confocal microscope (Leica Microsystems).
Results: Compared to GH peers, SH rats had lower microglial cell density in the HDG
after the 1st week of defined housing, which was not evident after the 2nd week. Other
parameters of microglia shape and ramification showed no difference between the
groups in neither of the two time points examined.
Conclusions: Obtained results showed that change in the cell density in the HDG was
the only and transient change in microglia morphology due to the social isolation
during adolescence. Further examination of other stress-related brain regions is
needed to define in depth the relationship between the microglia phenotype and earlylife
stress conditions.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Microglial morphological response to the lack of direct social contact in periadolescent rats",
pages = "108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5831"
}

Potrebić, M., Pavković, Ž., Srbovan, M., Dubljević, O.,& Pešić, V.. (2023). Microglial morphological response to the lack of direct social contact in periadolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 108.
https://hdl.handle.net/21.15107/rcub_ibiss_5831

Potrebić M, Pavković Ž, Srbovan M, Dubljević O, Pešić V. Microglial morphological response to the lack of direct social contact in periadolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:108.
https://hdl.handle.net/21.15107/rcub_ibiss_5831 .

Potrebić, Milica, Pavković, Željko, Srbovan, Maja, Dubljević, Olga, Pešić, Vesna, "Microglial morphological response to the lack of direct social contact in periadolescent rats" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):108,
https://hdl.handle.net/21.15107/rcub_ibiss_5831 .

TY  - CONF
AU  - Dubljević, Olga
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Potrebić, Milica
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5830
AB  - Parkinson's disease (PD) is characterized by both motor and non-motor symptoms.
Current research indicates that neuroinflammation, along with activated glial cells,
represents one of the key factors in the pathogenesis of PD. The aim of this study was
to assess non-motor symptoms of PD as well as the expression of gene markers of
microgliosis, astrogliosis and neuroinflammation in a transgenic A53T mouse model,
which is recommended for studying PD and other synucleinopathies. These transgenic
mice express an A53T missense mutant form of human alpha-synuclein under the
control of the murine prion promoter.
Depression-like symptoms and anhedonia were assessed using Tail Suspension Test
and Sucrose Preference Test respectively, while memory deficits were assessed using
Novel Object Recognition Test along with Object Relocation Test. Gene expression of
ionized calcium-binding adapter molecule (Iba-1), glial fibrillary acidic protein
(GFAP α and δ form) and cytokines associated with the microglial pro-inflammatory
phenotype (TNFα, IL-1β, IL-6) was assessed using RT-PCR. Brain regions of interest
were the prefrontal cortex, hippocampus, and striatum of 6 months old A53T male
mice. Non-transgenic littermates were used as controls. Obtained results suggest that
A53T mice compared to non-transgenic littermates exert 1) the presence of memory
deficits and anhedonia, 2) no difference in the expression of glial activity markers and
pro-inflammatory cytokines in the cortex and striatum, and 3) increased expression of
glial activity markers in the hippocampus, but without significant changes in the
expression of cytokines. Overall, better understanding of the role of active glia in the
model is needed.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5830
ER  - 

@conference{
author = "Dubljević, Olga and Pavković, Željko and Srbovan, Maja and Potrebić, Milica and Pešić, Vesna",
year = "2023",
abstract = "Parkinson's disease (PD) is characterized by both motor and non-motor symptoms.
Current research indicates that neuroinflammation, along with activated glial cells,
represents one of the key factors in the pathogenesis of PD. The aim of this study was
to assess non-motor symptoms of PD as well as the expression of gene markers of
microgliosis, astrogliosis and neuroinflammation in a transgenic A53T mouse model,
which is recommended for studying PD and other synucleinopathies. These transgenic
mice express an A53T missense mutant form of human alpha-synuclein under the
control of the murine prion promoter.
Depression-like symptoms and anhedonia were assessed using Tail Suspension Test
and Sucrose Preference Test respectively, while memory deficits were assessed using
Novel Object Recognition Test along with Object Relocation Test. Gene expression of
ionized calcium-binding adapter molecule (Iba-1), glial fibrillary acidic protein
(GFAP α and δ form) and cytokines associated with the microglial pro-inflammatory
phenotype (TNFα, IL-1β, IL-6) was assessed using RT-PCR. Brain regions of interest
were the prefrontal cortex, hippocampus, and striatum of 6 months old A53T male
mice. Non-transgenic littermates were used as controls. Obtained results suggest that
A53T mice compared to non-transgenic littermates exert 1) the presence of memory
deficits and anhedonia, 2) no difference in the expression of glial activity markers and
pro-inflammatory cytokines in the cortex and striatum, and 3) increased expression of
glial activity markers in the hippocampus, but without significant changes in the
expression of cytokines. Overall, better understanding of the role of active glia in the
model is needed.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5830"
}

Dubljević, O., Pavković, Ž., Srbovan, M., Potrebić, M.,& Pešić, V.. (2023). Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 109.
https://hdl.handle.net/21.15107/rcub_ibiss_5830

Dubljević O, Pavković Ž, Srbovan M, Potrebić M, Pešić V. Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:109.
https://hdl.handle.net/21.15107/rcub_ibiss_5830 .

Dubljević, Olga, Pavković, Željko, Srbovan, Maja, Potrebić, Milica, Pešić, Vesna, "Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):109,
https://hdl.handle.net/21.15107/rcub_ibiss_5830 .

TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Đmura, Goran
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5336
AB  - Changes in housing density, including individual housing, are commonly necessary in animal research. Obtaining reproducibility and translational validity in biomedical research requires an understanding of how animals adapt to changes in housing density. Existing literature mainly addresses acclimatization after transportation. We used a within-subject design to examine changes in behavior and weight gain of 4-mo-old male Wistar Han rats after reduction of their social group (RSG; due to removal of one rat from a cage containing 3 rats) and social isolation (SI; the removed rat) for the subsequent 2 wk. Changes in weight gain and in exploratory and center-avoidance behavior in an inescapable open arena (OA) were measured before (D0) and on days 7 and 14 (D7 and D14, respectively) after social change. The motor response to d-amphetamine (1.5 mg/kg), which stimulates behavioral arousal in response to novelty, was assessed at D14. Within-subject design revealed that RSG rats in OA had less locomotion at D7 but not more center-avoidance behavior and had returned to the D0 activity level at D14; SI rats in OA had consistently less locomotion and more center-avoidance behavior. Rearing behavior during OA exposure did not change in either group. However, SI rats showed more center-avoidance behavior in OA, greater weight gain, and less amphetamine-induced rearing at D14 as compared with RSG rats. These data indicate that after RSG, mature adult male rats require 2 wk to return to their baseline level of OA-related behavior, while after SI they gain weight and acquire maladaptive exploratory and center-avoidance behavior. The finding that SI produces maladaptive behavioral and physiologic alterations in adult male rats deserves attention because these changes could have confounding effects on research findings.
PB  - Memphis: American Association for Laboratory Animal Science
T2  - Journal of the American Association for Laboratory Animal Science
T1  - Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation
IS  - 6
VL  - 61
DO  - 10.30802/AALAS-JAALAS-22-000032
SP  - 615
EP  - 623
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Srbovan, Maja and Đmura, Goran and Pešić, Vesna",
year = "2022",
abstract = "Changes in housing density, including individual housing, are commonly necessary in animal research. Obtaining reproducibility and translational validity in biomedical research requires an understanding of how animals adapt to changes in housing density. Existing literature mainly addresses acclimatization after transportation. We used a within-subject design to examine changes in behavior and weight gain of 4-mo-old male Wistar Han rats after reduction of their social group (RSG; due to removal of one rat from a cage containing 3 rats) and social isolation (SI; the removed rat) for the subsequent 2 wk. Changes in weight gain and in exploratory and center-avoidance behavior in an inescapable open arena (OA) were measured before (D0) and on days 7 and 14 (D7 and D14, respectively) after social change. The motor response to d-amphetamine (1.5 mg/kg), which stimulates behavioral arousal in response to novelty, was assessed at D14. Within-subject design revealed that RSG rats in OA had less locomotion at D7 but not more center-avoidance behavior and had returned to the D0 activity level at D14; SI rats in OA had consistently less locomotion and more center-avoidance behavior. Rearing behavior during OA exposure did not change in either group. However, SI rats showed more center-avoidance behavior in OA, greater weight gain, and less amphetamine-induced rearing at D14 as compared with RSG rats. These data indicate that after RSG, mature adult male rats require 2 wk to return to their baseline level of OA-related behavior, while after SI they gain weight and acquire maladaptive exploratory and center-avoidance behavior. The finding that SI produces maladaptive behavioral and physiologic alterations in adult male rats deserves attention because these changes could have confounding effects on research findings.",
publisher = "Memphis: American Association for Laboratory Animal Science",
journal = "Journal of the American Association for Laboratory Animal Science",
title = "Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation",
number = "6",
volume = "61",
doi = "10.30802/AALAS-JAALAS-22-000032",
pages = "615-623"
}

Potrebić, M., Pavković, Ž., Srbovan, M., Đmura, G.,& Pešić, V.. (2022). Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation. in Journal of the American Association for Laboratory Animal Science
Memphis: American Association for Laboratory Animal Science., 61(6), 615-623.
https://doi.org/10.30802/AALAS-JAALAS-22-000032

Potrebić M, Pavković Ž, Srbovan M, Đmura G, Pešić V. Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation. in Journal of the American Association for Laboratory Animal Science. 2022;61(6):615-623.
doi:10.30802/AALAS-JAALAS-22-000032 .

Potrebić, Milica, Pavković, Željko, Srbovan, Maja, Đmura, Goran, Pešić, Vesna, "Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation" in Journal of the American Association for Laboratory Animal Science, 61, no. 6 (2022):615-623,
https://doi.org/10.30802/AALAS-JAALAS-22-000032 . .

TY  - CONF
AU  - Pavković, Željko
AU  - Potrebić, Milica
AU  - Dubljević, Olga
AU  - Srbovan, Maja
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5614
AB  - Познато је да су анксиозни поремећаји генерално изражени током адолесценције док специфичности везане за интензитет симптома током различитих фаза адолесценције (рана, средња, касна) још увек нису довољно проучене. Aдолесценција је као период матурације присутна код свих сисара, што оправдава коришћење анималних модела у психобиологији. Имајући у виду дефинисане фазе адолесценције глодара, испитивали смо матурацију експлораторног и анксиозног одговора на новину код мужјака Wistar Han пацова старих 35 ± 1 дан (крај ране/почетак средње адолесценције), 49 ± 1 дан (крај средње/почетак касне адолесценције) и 155 ± 1 дан (адултно доба). Коришћени су тестови у којима животиња слободно истражује нову средину (нова правоугаона арена, црно-бела кутија, уздигнути крстасти лавиринт). Адаптабилност на нову средину је процењивана поређењем бихејвиоралних одговора у првих и последњих 5 минута тестирања. Добијени резултати су показали да код пацова 1) матурациона крива експлораторног одговора на нову средину може имати како линеарни тако и U-профил, у зависности од карактеристика новe средине, 2) понашање налик анксиозном има инвертовани U-профил, док се 3) прилагодљивост независно од срединских услова јавља крајем средње/почетком касне адолесценције. Дискутован је потенцијални значај добијених резултата.
AB  - Poznato je da su anksiozni poremećaji generalno izraženi tokom adolescencije dok specifičnosti vezane za intenzitet simptoma tokom različitih faza adolescencije (rana, srednja, kasna) još uvek nisu dovoljno proučene. Adolescencija je kao period maturacije prisutna kod svih sisara, što opravdava korišćenje animalnih modela u psihobiologiji. Imajući u vidu definisane faze adolescencije glodara, ispitivali smo maturaciju eksploratornog i anksioznog odgovora na novinu kod mužjaka Wistar Han pacova starih 35 ± 1 dan (kraj rane/početak srednje adolescencije), 49 ± 1 dan (kraj srednje/početak kasne adolescencije) i 155 ± 1 dan (adultno doba). Korišćeni su testovi u kojima životinja slobodno istražuje novu sredinu (nova pravougaona arena, crno-bela kutija, uzdignuti krstasti lavirint). Adaptabilnost na novu sredinu je procenjivana poređenjem bihejvioralnih odgovora u prvih i poslednjih 5 minuta testiranja. Dobijeni rezultati su pokazali da kod pacova 1) maturaciona kriva eksploratornog odgovora na novu sredinu može imati kako linearni tako i U-profil, u zavisnosti od karakteristika nove sredine, 2) ponašanje nalik anksioznom ima invertovani U-profil, dok se 3) prilagodljivost nezavisno od sredinskih uslova javlja krajem srednje/početkom kasne adolescencije. Diskutovan je potencijalni značaj dobijenih rezultata.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова
T1  - Maturacija eksploratornog, anksioznog i adaptivnog odgovora na nove sredine u modelu pacova
SP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5614
ER  - 

@conference{
author = "Pavković, Željko and Potrebić, Milica and Dubljević, Olga and Srbovan, Maja and Pešić, Vesna",
year = "2022",
abstract = "Познато је да су анксиозни поремећаји генерално изражени током адолесценције док специфичности везане за интензитет симптома током различитих фаза адолесценције (рана, средња, касна) још увек нису довољно проучене. Aдолесценција је као период матурације присутна код свих сисара, што оправдава коришћење анималних модела у психобиологији. Имајући у виду дефинисане фазе адолесценције глодара, испитивали смо матурацију експлораторног и анксиозног одговора на новину код мужјака Wistar Han пацова старих 35 ± 1 дан (крај ране/почетак средње адолесценције), 49 ± 1 дан (крај средње/почетак касне адолесценције) и 155 ± 1 дан (адултно доба). Коришћени су тестови у којима животиња слободно истражује нову средину (нова правоугаона арена, црно-бела кутија, уздигнути крстасти лавиринт). Адаптабилност на нову средину је процењивана поређењем бихејвиоралних одговора у првих и последњих 5 минута тестирања. Добијени резултати су показали да код пацова 1) матурациона крива експлораторног одговора на нову средину може имати како линеарни тако и U-профил, у зависности од карактеристика новe средине, 2) понашање налик анксиозном има инвертовани U-профил, док се 3) прилагодљивост независно од срединских услова јавља крајем средње/почетком касне адолесценције. Дискутован је потенцијални значај добијених резултата., Poznato je da su anksiozni poremećaji generalno izraženi tokom adolescencije dok specifičnosti vezane za intenzitet simptoma tokom različitih faza adolescencije (rana, srednja, kasna) još uvek nisu dovoljno proučene. Adolescencija je kao period maturacije prisutna kod svih sisara, što opravdava korišćenje animalnih modela u psihobiologiji. Imajući u vidu definisane faze adolescencije glodara, ispitivali smo maturaciju eksploratornog i anksioznog odgovora na novinu kod mužjaka Wistar Han pacova starih 35 ± 1 dan (kraj rane/početak srednje adolescencije), 49 ± 1 dan (kraj srednje/početak kasne adolescencije) i 155 ± 1 dan (adultno doba). Korišćeni su testovi u kojima životinja slobodno istražuje novu sredinu (nova pravougaona arena, crno-bela kutija, uzdignuti krstasti lavirint). Adaptabilnost na novu sredinu je procenjivana poređenjem bihejvioralnih odgovora u prvih i poslednjih 5 minuta testiranja. Dobijeni rezultati su pokazali da kod pacova 1) maturaciona kriva eksploratornog odgovora na novu sredinu može imati kako linearni tako i U-profil, u zavisnosti od karakteristika nove sredine, 2) ponašanje nalik anksioznom ima invertovani U-profil, dok se 3) prilagodljivost nezavisno od sredinskih uslova javlja krajem srednje/početkom kasne adolescencije. Diskutovan je potencijalni značaj dobijenih rezultata.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова, Maturacija eksploratornog, anksioznog i adaptivnog odgovora na nove sredine u modelu pacova",
pages = "339",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5614"
}

Pavković, Ž., Potrebić, M., Dubljević, O., Srbovan, M.,& Pešić, V.. (2022). Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 339.
https://hdl.handle.net/21.15107/rcub_ibiss_5614

Pavković Ž, Potrebić M, Dubljević O, Srbovan M, Pešić V. Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:339.
https://hdl.handle.net/21.15107/rcub_ibiss_5614 .

Pavković, Željko, Potrebić, Milica, Dubljević, Olga, Srbovan, Maja, Pešić, Vesna, "Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):339,
https://hdl.handle.net/21.15107/rcub_ibiss_5614 .

TY  - CONF
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5615
AB  - Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење и 
најчешћи узрок деменције код старих особа, са преваленцом два пута већом у 
женској популацији.  Упркос дугогодишњим истраживањима механизама 
патогенезе АБ, као и бројним спроведеним претклиничким студијама, још увек не 
постоји адекватна терапија за ово обољење. У овој иницијалној студији испитиван 
је неуропротективни потенцијал нано квантне супстанце 3HFWC  –  хипер-
хармонизованог  комплекса  хидроксилованог фулерена и воде, у  животињском 
моделу АБ  –  трансгеним  5XFAD мишевима. Женке 5XFAD мишева  су  излагане 
нано квантној супстанци 3HFWC у продромалној фази патологије. Третман је 
започет када су животиње биле старе 4 недеље и животиње су појене раствором 
нано квантне  супстанце 3HFWC  уместо воде током наредна три месеца.  Након 
третмана, анализирани су број и морфолошке карактеристике амилоидних плакова 
у структурама мозга од значаја за процесе учења и памћења – кори великог мозга и 
хипокампусу. Испитиван је и ефекат третмана на акумулацију токсичног протеина 
амилоида бета (Аβ), као и промене у маркерима синаптичке пластичности. Третман 
са 3HFWC је значајно смањио заступљеност амилоидних плакова у одређеним 
регионима коре великог мозга. Резултати стога указују  на неуропротективно 
дејство превентивне примене  нано  квантне  супстанце 3HFWC  у  мишјем моделу 
Алцхајмерове болести.
AB  - Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje i najčešći uzrok demencije kod starih osoba, sa prevalencom dva puta većom u ženskoj populaciji. Uprkos dugogodišnjim istraživanjima mehanizama patogeneze AB, kao i brojnim sprovedenim pretkliničkim studijama, još uvek ne postoji adekvatna terapija za ovo oboljenje. U ovoj inicijalnoj studiji ispitivan je neuroprotektivni potencijal nano kvantne supstance 3HFWC – hiper- harmonizovanog kompleksa hidroksilovanog fulerena i vode, u životinjskom modelu AB – transgenim 5XFAD miševima. Ženke 5XFAD miševa su izlagane nano kvantnoj supstanci 3HFWC u prodromalnoj fazi patologije. Tretman je započet kada su životinje bile stare 4 nedelje i životinje su pojene rastvorom nano kvantne supstance 3HFWC umesto vode tokom naredna tri meseca. Nakon tretmana, analizirani su broj i morfološke karakteristike amiloidnih plakova u strukturama mozga od značaja za procese učenja i pamćenja – kori velikog mozga i hipokampusu. Ispitivan je i efekat tretmana na akumulaciju toksičnog proteina amiloida beta (Aβ), kao i promene u markerima sinaptičke plastičnosti. Tretman sa 3HFWC je značajno smanjio zastupljenost amiloidnih plakova u određenim regionima kore velikog mozga. Rezultati stoga ukazuju na neuroprotektivno dejstvo preventivne primene nano kvantne supstance 3HFWC u mišjem modelu Alchajmerove bolesti.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti
T1  - Испитивање неуропротективног потенцијала наноквантне супстанце 3HFWC у мишијем моделу Алцхајмерове болести
SP  - 317
SP  - M64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5615
ER  - 

@conference{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "Алцхајмерова болест (АБ) је прогресивно неуродегенеративно обољење и 
најчешћи узрок деменције код старих особа, са преваленцом два пута већом у 
женској популацији.  Упркос дугогодишњим истраживањима механизама 
патогенезе АБ, као и бројним спроведеним претклиничким студијама, још увек не 
постоји адекватна терапија за ово обољење. У овој иницијалној студији испитиван 
је неуропротективни потенцијал нано квантне супстанце 3HFWC  –  хипер-
хармонизованог  комплекса  хидроксилованог фулерена и воде, у  животињском 
моделу АБ  –  трансгеним  5XFAD мишевима. Женке 5XFAD мишева  су  излагане 
нано квантној супстанци 3HFWC у продромалној фази патологије. Третман је 
започет када су животиње биле старе 4 недеље и животиње су појене раствором 
нано квантне  супстанце 3HFWC  уместо воде током наредна три месеца.  Након 
третмана, анализирани су број и морфолошке карактеристике амилоидних плакова 
у структурама мозга од значаја за процесе учења и памћења – кори великог мозга и 
хипокампусу. Испитиван је и ефекат третмана на акумулацију токсичног протеина 
амилоида бета (Аβ), као и промене у маркерима синаптичке пластичности. Третман 
са 3HFWC је значајно смањио заступљеност амилоидних плакова у одређеним 
регионима коре великог мозга. Резултати стога указују  на неуропротективно 
дејство превентивне примене  нано  квантне  супстанце 3HFWC  у  мишјем моделу 
Алцхајмерове болести., Alchajmerova bolest (AB) je progresivno neurodegenerativno oboljenje i najčešći uzrok demencije kod starih osoba, sa prevalencom dva puta većom u ženskoj populaciji. Uprkos dugogodišnjim istraživanjima mehanizama patogeneze AB, kao i brojnim sprovedenim pretkliničkim studijama, još uvek ne postoji adekvatna terapija za ovo oboljenje. U ovoj inicijalnoj studiji ispitivan je neuroprotektivni potencijal nano kvantne supstance 3HFWC – hiper- harmonizovanog kompleksa hidroksilovanog fulerena i vode, u životinjskom modelu AB – transgenim 5XFAD miševima. Ženke 5XFAD miševa su izlagane nano kvantnoj supstanci 3HFWC u prodromalnoj fazi patologije. Tretman je započet kada su životinje bile stare 4 nedelje i životinje su pojene rastvorom nano kvantne supstance 3HFWC umesto vode tokom naredna tri meseca. Nakon tretmana, analizirani su broj i morfološke karakteristike amiloidnih plakova u strukturama mozga od značaja za procese učenja i pamćenja – kori velikog mozga i hipokampusu. Ispitivan je i efekat tretmana na akumulaciju toksičnog proteina amiloida beta (Aβ), kao i promene u markerima sinaptičke plastičnosti. Tretman sa 3HFWC je značajno smanjio zastupljenost amiloidnih plakova u određenim regionima kore velikog mozga. Rezultati stoga ukazuju na neuroprotektivno dejstvo preventivne primene nano kvantne supstance 3HFWC u mišjem modelu Alchajmerove bolesti.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti, Испитивање неуропротективног потенцијала наноквантне супстанце 3HFWC у мишијем моделу Алцхајмерове болести",
pages = "317-M64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5615"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2022). Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 317.
https://hdl.handle.net/21.15107/rcub_ibiss_5615

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:317.
https://hdl.handle.net/21.15107/rcub_ibiss_5615 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "Ispitivanje neuroprotektivnog potencijala nanokvantne supstance 3HFWC u mišijem modelu Alchajmerove bolesti" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):317,
https://hdl.handle.net/21.15107/rcub_ibiss_5615 .

TY  - CONF
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Matović, Valentina
AU  - Srbovan, Maja
AU  - Koruga, Đuro
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5188
AB  - The potential of fullerenes and fullerene’s water-soluble derivatives
to bind to amyloid-b has been well documented in vitro and
in silico. However, the anti-amyloid action of fullerenols in
in vivo treatments has not been fully examined. In the present study we assessed the effects of the hydroxylated fullerene-water
complex (3HFWC) on Alzheimer’s disease (AD) neuropathological
hallmarks in 5XFAD mice, a well-recognized AD animal
model. The 3-week-old 5XFAD mice were exposed to 3HFWC
water solution ad libitum for 3 months. The 3HFWC treatment
started in the presymptomatic phase of pathology and analyses
were focused on the effects on amyloid-b (Ab) accumulation, plaque
formation, gliosis, and synaptic plasticity in cortical and hippocampal
tissue. The 3HFWC treatment significantly decreased
the amyloid-b plaque load in specific parts of cerebral cortex, followed
by the unchanged levels of Ab. None of these changes
were detected in the hippocampus. At the same time, 3HFWC
treatment did not exacerbate the activation of glial cells, nor
altered the expression levels of synaptic proteins. The obtained
results point to the potential of 3HFWC, when applied in the
presymptomatic phase of AD, to interfere with Ab accumulation
and amyloid plaque formation without exacerbating the other
AD-related pathological processes.
PB  - Hoboken : John Wiley & Sons Ltd
C3  - The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
T1  - Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease
DO  - 10.1002/2211-5463.13440
SP  - 130
ER  - 

@conference{
author = "Perović, Milka and Ćirić, Jelena and Matović, Valentina and Srbovan, Maja and Koruga, Đuro and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The potential of fullerenes and fullerene’s water-soluble derivatives
to bind to amyloid-b has been well documented in vitro and
in silico. However, the anti-amyloid action of fullerenols in
in vivo treatments has not been fully examined. In the present study we assessed the effects of the hydroxylated fullerene-water
complex (3HFWC) on Alzheimer’s disease (AD) neuropathological
hallmarks in 5XFAD mice, a well-recognized AD animal
model. The 3-week-old 5XFAD mice were exposed to 3HFWC
water solution ad libitum for 3 months. The 3HFWC treatment
started in the presymptomatic phase of pathology and analyses
were focused on the effects on amyloid-b (Ab) accumulation, plaque
formation, gliosis, and synaptic plasticity in cortical and hippocampal
tissue. The 3HFWC treatment significantly decreased
the amyloid-b plaque load in specific parts of cerebral cortex, followed
by the unchanged levels of Ab. None of these changes
were detected in the hippocampus. At the same time, 3HFWC
treatment did not exacerbate the activation of glial cells, nor
altered the expression levels of synaptic proteins. The obtained
results point to the potential of 3HFWC, when applied in the
presymptomatic phase of AD, to interfere with Ab accumulation
and amyloid plaque formation without exacerbating the other
AD-related pathological processes.",
publisher = "Hoboken : John Wiley & Sons Ltd",
journal = "The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal",
title = "Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease",
doi = "10.1002/2211-5463.13440",
pages = "130"
}

Perović, M., Ćirić, J., Matović, V., Srbovan, M., Koruga, Đ., Kanazir, S.,& Ivković, S.. (2022). Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal
Hoboken : John Wiley & Sons Ltd., 130.
https://doi.org/10.1002/2211-5463.13440

Perović M, Ćirić J, Matović V, Srbovan M, Koruga Đ, Kanazir S, Ivković S. Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease. in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal. 2022;:130.
doi:10.1002/2211-5463.13440 .

Perović, Milka, Ćirić, Jelena, Matović, Valentina, Srbovan, Maja, Koruga, Đuro, Kanazir, Selma, Ivković, Sanja, "Composition comprising hydroxyl modified fullerene substances decrease plaque load in 5XFAD mouse model of Alzheimer's disease" in The Biochemistry Global Summit: 25th IUBMB Congress: 46th FEBS Congress: 15th PABMB Congress; 2022 Jul 9-14; Lisbon, Portugal (2022):130,
https://doi.org/10.1002/2211-5463.13440 . .

TY  - CONF
AU  - Srbovan, Maja
AU  - Prpa, Ksenija
AU  - Tešić, Vesna
AU  - Milanović, Desanka
AU  - Perović, Milka
AU  - Kanazir, Selma
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5908
AB  - Aim: Food restriction has been widely associated with beneficial effects on brain aging and age-related neurodegenerative diseases such as Alzheimer’s disease (AD). In the present study, the effects of every-other-day (EOD) feeding regimen were studied in the hippocampus of 5XFAD mice, a well characterized animal model of AD. Parvalbumin (PV) inhibitory interneurons that are crucial for maintaining proper excitatory/inhibitory balance were examined.
Methods: Female 5xFAD mice (Tg) and their non-transgenic littermates (non-Tg) were exposed to ad libitum (AL) or intermittent, EOD feeding regimen, beginning at 2 months of age. Neurons expressing PV were detected by immunohistochemistry, in the dorsal hippocampus of 6-month-old animals. The number of parvalbumin-expressing neurons was determined independently in CA1, CA3, and DG hippocampal subregions.
Results: Immunohistochemical analysis revealed a substantial increase in the number of parvalbumin inhibitory neurons in the dorsal hippocampus of Tg-AL mice in comparison to non-Tg animals. In Tg-EOD mice, however, alterations in the number of PV-expressing neurons were subregion-specific comparing to Tg-AL mice of the same age.
Conlusions: The results of our study clearly indicate that PV-expressing interneurons are of importance in further understanding of neural basis of AD-like-associated cognitive impairments and EOD-induced effects in 5xFAD mouse model of AD.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
T1  - The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice
IS  - 299
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5908
ER  - 

@conference{
author = "Srbovan, Maja and Prpa, Ksenija and Tešić, Vesna and Milanović, Desanka and Perović, Milka and Kanazir, Selma",
year = "2019",
abstract = "Aim: Food restriction has been widely associated with beneficial effects on brain aging and age-related neurodegenerative diseases such as Alzheimer’s disease (AD). In the present study, the effects of every-other-day (EOD) feeding regimen were studied in the hippocampus of 5XFAD mice, a well characterized animal model of AD. Parvalbumin (PV) inhibitory interneurons that are crucial for maintaining proper excitatory/inhibitory balance were examined.
Methods: Female 5xFAD mice (Tg) and their non-transgenic littermates (non-Tg) were exposed to ad libitum (AL) or intermittent, EOD feeding regimen, beginning at 2 months of age. Neurons expressing PV were detected by immunohistochemistry, in the dorsal hippocampus of 6-month-old animals. The number of parvalbumin-expressing neurons was determined independently in CA1, CA3, and DG hippocampal subregions.
Results: Immunohistochemical analysis revealed a substantial increase in the number of parvalbumin inhibitory neurons in the dorsal hippocampus of Tg-AL mice in comparison to non-Tg animals. In Tg-EOD mice, however, alterations in the number of PV-expressing neurons were subregion-specific comparing to Tg-AL mice of the same age.
Conlusions: The results of our study clearly indicate that PV-expressing interneurons are of importance in further understanding of neural basis of AD-like-associated cognitive impairments and EOD-induced effects in 5xFAD mouse model of AD.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia",
title = "The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice",
number = "299",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5908"
}

Srbovan, M., Prpa, K., Tešić, V., Milanović, D., Perović, M.,& Kanazir, S.. (2019). The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society.(299).
https://hdl.handle.net/21.15107/rcub_ibiss_5908

Srbovan M, Prpa K, Tešić V, Milanović D, Perović M, Kanazir S. The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice. in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia. 2019;(299).
https://hdl.handle.net/21.15107/rcub_ibiss_5908 .

Srbovan, Maja, Prpa, Ksenija, Tešić, Vesna, Milanović, Desanka, Perović, Milka, Kanazir, Selma, "The effect of intermittent feeding on the number of parvalbumin-expressing neurons in the hippocampus of 5XFAD mice" in Book of Abstract: Federation of European Neuroscience Societies (FENS) Regional Meeting; 2019 Jul 10-13; Belgrade, Serbia, no. 299 (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5908 .