TY  - JOUR
AU  - Heberger, Karoly
AU  - Kolarevic, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Sunjog, Karolina
AU  - Gacic, Zoran
AU  - Kljajic, Zoran
AU  - Mitric, Milena
AU  - Vukovic-Gacic, Branka
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2165
AB  - Specimens of the mussel Mytilus galloprovincialis were collected from
   five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during
   the period summer 2011-autumn 2012. Three types of tissue, haemolymph,
   digestive gland were used for assessment of DNA damage. Images of
   randomly selected cells were analyzed with a fluorescence microscope and
   image analysis by the Comet Assay IV Image-analysis system. Three
   parameters, viz, tail length, tail intensity and Olive tail moment were
   analyzed on 4200 nuclei per cell type. We observed variations in the
   level of DNA damage in mussels collected at different sites, as well as
   seasonal variations in response. Sum of ranking differences (SRD) was
   implemented to compare use of different types of cell and different
   measure of comet tail per nucleus. Numerical scales were transferred
   into ranks, range scaling between 0 and 1; standardization and
   normalization were carried out.
   SRD selected the best (and worst) combinations: tail moment is the best
   for all data treatment and for all organs; second best is tail length,
   and intensity ranks third (except for digestive gland). The differences
   were significant at the 5\% level. Whereas gills and haemolymph cells do
   not differ significantly, cells of the digestive gland are much more
   suitable to estimate genotoxicity. Variance analysis decomposed the
   effect of different factors on the SRD values. This unique combination
   has provided not only the relative importance of factors, but also an
   overall evaluation: the best evaluation method, the best data
   pre-treatment, etc., were chosen even for partially contradictory data.
   The rank transformation is superior to any other way of scaling, which
   is proven by ordering the SRD values by SRD again, and by cross
   validation. (C) 2014 Elsevier B.V. All rights reserved.
T2  - Mutation Research-Genetic Toxicology and Environmental Mutagenesis
T1  - Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences
VL  - 771
DO  - 10.1016/j.mrgentox.2014.04.028
SP  - 15
EP  - 22
ER  - 

@article{
author = "Heberger, Karoly and Kolarevic, Stoimir and Kračun-Kolarević, Margareta and Sunjog, Karolina and Gacic, Zoran and Kljajic, Zoran and Mitric, Milena and Vukovic-Gacic, Branka",
year = "2014",
abstract = "Specimens of the mussel Mytilus galloprovincialis were collected from
   five sites in the Boka Kotorska Bay (Adriatic Sea, Montenegro) during
   the period summer 2011-autumn 2012. Three types of tissue, haemolymph,
   digestive gland were used for assessment of DNA damage. Images of
   randomly selected cells were analyzed with a fluorescence microscope and
   image analysis by the Comet Assay IV Image-analysis system. Three
   parameters, viz, tail length, tail intensity and Olive tail moment were
   analyzed on 4200 nuclei per cell type. We observed variations in the
   level of DNA damage in mussels collected at different sites, as well as
   seasonal variations in response. Sum of ranking differences (SRD) was
   implemented to compare use of different types of cell and different
   measure of comet tail per nucleus. Numerical scales were transferred
   into ranks, range scaling between 0 and 1; standardization and
   normalization were carried out.
   SRD selected the best (and worst) combinations: tail moment is the best
   for all data treatment and for all organs; second best is tail length,
   and intensity ranks third (except for digestive gland). The differences
   were significant at the 5\% level. Whereas gills and haemolymph cells do
   not differ significantly, cells of the digestive gland are much more
   suitable to estimate genotoxicity. Variance analysis decomposed the
   effect of different factors on the SRD values. This unique combination
   has provided not only the relative importance of factors, but also an
   overall evaluation: the best evaluation method, the best data
   pre-treatment, etc., were chosen even for partially contradictory data.
   The rank transformation is superior to any other way of scaling, which
   is proven by ordering the SRD values by SRD again, and by cross
   validation. (C) 2014 Elsevier B.V. All rights reserved.",
journal = "Mutation Research-Genetic Toxicology and Environmental Mutagenesis",
title = "Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences",
volume = "771",
doi = "10.1016/j.mrgentox.2014.04.028",
pages = "15-22"
}

Heberger, K., Kolarevic, S., Kračun-Kolarević, M., Sunjog, K., Gacic, Z., Kljajic, Z., Mitric, M.,& Vukovic-Gacic, B.. (2014). Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 771, 15-22.
https://doi.org/10.1016/j.mrgentox.2014.04.028

Heberger K, Kolarevic S, Kračun-Kolarević M, Sunjog K, Gacic Z, Kljajic Z, Mitric M, Vukovic-Gacic B. Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences. in Mutation Research-Genetic Toxicology and Environmental Mutagenesis. 2014;771:15-22.
doi:10.1016/j.mrgentox.2014.04.028 .

Heberger, Karoly, Kolarevic, Stoimir, Kračun-Kolarević, Margareta, Sunjog, Karolina, Gacic, Zoran, Kljajic, Zoran, Mitric, Milena, Vukovic-Gacic, Branka, "Evaluation of single-cell gel electrophoresis data: Combination of
 variance analysis with sum of ranking differences" in Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 771 (2014):15-22,
https://doi.org/10.1016/j.mrgentox.2014.04.028 . .