TY  - JOUR
AU  - Bjedov, Ivana
AU  - Obratov-Petkovic, Dragica
AU  - Misic, Danijela
AU  - Šiler, Branislav
AU  - Aleksic, Jelena M.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2050
AB  - Vaccinium myrtillus L., Vaccinium uliginosum L. and Vaccinium
   vitis-idaea L. are perennial, cold-adapted clonal shrubs distributed
   throughout Europe, northern Asia and North America. Due to their usage
   in food (berries) and pharmaceutical industry (berries and leaves),
   their natural populations are exposed to anthropogenic and other impacts
   that affect their genetic make-up. We analyzed 14 fragmentary
   distributed and small-sized peripheral populations of these species from
   the Balkans, which represents the southeastern-European marginal area of
   their wide European distributions, using RAPD molecular markers. The
   contemporary genetic patterns in all three species within the Balkans
   were generally similar, and in comparison to previous reports on
   populations of these species found in northward Europe, where they have
   a more continuous distribution, the levels of genetic diversity were
   more or less halved, genetic differentiation was several times higher,
   gene flow exceptionally low, and the expected prevalence of clonal
   individuals was lacking. The population dynamics of all three species
   within the Balkans was complex and distinct, and was characterized by a
   past admixture of individuals from discrete populations of the same
   species and interspecific hybridisation not only between V. myrtillus
   and V. vitis-idaea but also between V. uliginosum and V. vitis-idaea,
   the latter not being reported to date. Conservation measures suitable
   for preservation of presumably genetically distinct portions of the
   Balkans' gene pools of studied species have been suggested, while the
   utility of interspecific hybrids in breeding programs and/or in
   food/pharmaceutical industry is yet to be assessed.
T2  - Silva Fennica
T1  - Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage
IS  - 1283
VL  - 49
DO  - 10.14214/sf.1283
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2050
ER  - 

@article{
author = "Bjedov, Ivana and Obratov-Petkovic, Dragica and Misic, Danijela and Šiler, Branislav and Aleksic, Jelena M.",
year = "2015",
abstract = "Vaccinium myrtillus L., Vaccinium uliginosum L. and Vaccinium
   vitis-idaea L. are perennial, cold-adapted clonal shrubs distributed
   throughout Europe, northern Asia and North America. Due to their usage
   in food (berries) and pharmaceutical industry (berries and leaves),
   their natural populations are exposed to anthropogenic and other impacts
   that affect their genetic make-up. We analyzed 14 fragmentary
   distributed and small-sized peripheral populations of these species from
   the Balkans, which represents the southeastern-European marginal area of
   their wide European distributions, using RAPD molecular markers. The
   contemporary genetic patterns in all three species within the Balkans
   were generally similar, and in comparison to previous reports on
   populations of these species found in northward Europe, where they have
   a more continuous distribution, the levels of genetic diversity were
   more or less halved, genetic differentiation was several times higher,
   gene flow exceptionally low, and the expected prevalence of clonal
   individuals was lacking. The population dynamics of all three species
   within the Balkans was complex and distinct, and was characterized by a
   past admixture of individuals from discrete populations of the same
   species and interspecific hybridisation not only between V. myrtillus
   and V. vitis-idaea but also between V. uliginosum and V. vitis-idaea,
   the latter not being reported to date. Conservation measures suitable
   for preservation of presumably genetically distinct portions of the
   Balkans' gene pools of studied species have been suggested, while the
   utility of interspecific hybrids in breeding programs and/or in
   food/pharmaceutical industry is yet to be assessed.",
journal = "Silva Fennica",
title = "Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage",
number = "1283",
volume = "49",
doi = "10.14214/sf.1283",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2050"
}

Bjedov, I., Obratov-Petkovic, D., Misic, D., Šiler, B.,& Aleksic, J. M.. (2015). Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage. in Silva Fennica, 49(1283).
https://doi.org/10.14214/sf.1283
https://hdl.handle.net/21.15107/rcub_ibiss_2050

Bjedov I, Obratov-Petkovic D, Misic D, Šiler B, Aleksic JM. Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage. in Silva Fennica. 2015;49(1283).
doi:10.14214/sf.1283
https://hdl.handle.net/21.15107/rcub_ibiss_2050 .

Bjedov, Ivana, Obratov-Petkovic, Dragica, Misic, Danijela, Šiler, Branislav, Aleksic, Jelena M., "Genetic patterns in range-edge populations of Vaccinium species from the
 central Balkans: implications on conservation prospects and sustainable
 usage" in Silva Fennica, 49, no. 1283 (2015),
https://doi.org/10.14214/sf.1283 .,
https://hdl.handle.net/21.15107/rcub_ibiss_2050 .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Aleksic, Jelena M.
AU  - Dodos, Tanja
AU  - Rajcevic, Nemanja
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2063
AB  - Due to the current lack of nuclear microsatellites (simple sequence
   repeats SSRs) specifically developed for Pinus nigra, an important
   European coniferous species, we cross-species amplified 12 EST-SSRs
   (expressed sequence tagged SSRs) developed for other Pinus species in P.
   nigra in order to delineate loci which can be used for assessing levels
   of genetic diversity and genetic structuring in this species. We
   amplified these loci in individuals from seven populations from the
   central Balkans representing four recognized infraspecific taxa of P.
   nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica).
   Contrary to expectations on high transferability of EST-SSRs into
   related species, only three out of 12 tested loci were successfully
   amplified in P. nigra, but they displayed lack/low levels of
   polymorphism or generated multilocus amplification products. Thus, our
   estimates on levels of genetic diversity (HE = 0.183) and genetic
   differentiation (F-ST = 0.007) were based on variability of a single
   locus harboring four alleles only and they should be taken with
   cautions. Our study highlights the need for the development of
   high-resolution molecular markers, such as co-dominant genic or genomic
   SSRs or predominantly biallelic SNPs, or utilization of anonymous
   dominant markers, such as AFLPs, for genotyping in P. nigra.
T2  - Genetika-Belgrade
T1  - CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra
IS  - 1
VL  - 47
DO  - 10.2298/GENSR1501205S
SP  - 205
EP  - 217
ER  - 

@article{
author = "Sarac, Zorica and Aleksic, Jelena M. and Dodos, Tanja and Rajcevic, Nemanja and Bojović, Srđan and Marin, Petar D.",
year = "2015",
abstract = "Due to the current lack of nuclear microsatellites (simple sequence
   repeats SSRs) specifically developed for Pinus nigra, an important
   European coniferous species, we cross-species amplified 12 EST-SSRs
   (expressed sequence tagged SSRs) developed for other Pinus species in P.
   nigra in order to delineate loci which can be used for assessing levels
   of genetic diversity and genetic structuring in this species. We
   amplified these loci in individuals from seven populations from the
   central Balkans representing four recognized infraspecific taxa of P.
   nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica).
   Contrary to expectations on high transferability of EST-SSRs into
   related species, only three out of 12 tested loci were successfully
   amplified in P. nigra, but they displayed lack/low levels of
   polymorphism or generated multilocus amplification products. Thus, our
   estimates on levels of genetic diversity (HE = 0.183) and genetic
   differentiation (F-ST = 0.007) were based on variability of a single
   locus harboring four alleles only and they should be taken with
   cautions. Our study highlights the need for the development of
   high-resolution molecular markers, such as co-dominant genic or genomic
   SSRs or predominantly biallelic SNPs, or utilization of anonymous
   dominant markers, such as AFLPs, for genotyping in P. nigra.",
journal = "Genetika-Belgrade",
title = "CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501205S",
pages = "205-217"
}

Sarac, Z., Aleksic, J. M., Dodos, T., Rajcevic, N., Bojović, S.,& Marin, P. D.. (2015). CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra. in Genetika-Belgrade, 47(1), 205-217.
https://doi.org/10.2298/GENSR1501205S

Sarac Z, Aleksic JM, Dodos T, Rajcevic N, Bojović S, Marin PD. CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra. in Genetika-Belgrade. 2015;47(1):205-217.
doi:10.2298/GENSR1501205S .

Sarac, Zorica, Aleksic, Jelena M., Dodos, Tanja, Rajcevic, Nemanja, Bojović, Srđan, Marin, Petar D., "CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra" in Genetika-Belgrade, 47, no. 1 (2015):205-217,
https://doi.org/10.2298/GENSR1501205S . .

TY  - JOUR
AU  - Šarac, Zorica
AU  - Dodos, Tanja
AU  - Rajčević, Nemanja
AU  - Bojović, Srđan
AU  - Marin, Petar D.
AU  - Aleksić, Jelena M.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2040
AB  - Pinus nigra J.F. Arnold, European black pine, is a typical component of
   Mediterranean and sub-Mediterranean coniferous forests with highly
   fragmentary distribution. Western Mediterranean populations of this
   species have been studied genetically to date, while eastern populations
   from the central Balkans, which are larger and more abundant, are still
   genetically understudied. We analyzed seven populations of P. nigra
   representing all infraspecific taxa recognized within the central
   Balkans (subspecies nigra with varieties nigra and gocensis Dordevic;
   and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and
   banatica (Endl.) Georgescu et Ionescu), with three chloroplast
   microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus.
   Although our molecular data failed to support circumscription of studied
   infraspecific taxa, we found that genetic patterns at both genomes are
   in accordance with those found previously in westward populations of
   this species, that is - exceptionally high levels of genetic diversity
   (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the
   cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and
   genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on
   genealogical relations of mtDNA types currently present in Balkans' and
   Iberian/African populations, we inferred that the ancestral gene pool of
   P. nigra already harbored polymorphism at position 328 prior to the
   divergence to two lineages currently present in westward and eastward
   parts of the species range distribution. Subsequent occurrence of three
   mutations, which distinguish these two lineages, suggests their
   long-term isolation.
T2  - Silva Fennica
T1  - Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data
IS  - 1415
VL  - 49
DO  - 10.14214/sf.1415
ER  - 

@article{
author = "Šarac, Zorica and Dodos, Tanja and Rajčević, Nemanja and Bojović, Srđan and Marin, Petar D. and Aleksić, Jelena M.",
year = "2015",
abstract = "Pinus nigra J.F. Arnold, European black pine, is a typical component of
   Mediterranean and sub-Mediterranean coniferous forests with highly
   fragmentary distribution. Western Mediterranean populations of this
   species have been studied genetically to date, while eastern populations
   from the central Balkans, which are larger and more abundant, are still
   genetically understudied. We analyzed seven populations of P. nigra
   representing all infraspecific taxa recognized within the central
   Balkans (subspecies nigra with varieties nigra and gocensis Dordevic;
   and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and
   banatica (Endl.) Georgescu et Ionescu), with three chloroplast
   microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus.
   Although our molecular data failed to support circumscription of studied
   infraspecific taxa, we found that genetic patterns at both genomes are
   in accordance with those found previously in westward populations of
   this species, that is - exceptionally high levels of genetic diversity
   (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the
   cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and
   genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on
   genealogical relations of mtDNA types currently present in Balkans' and
   Iberian/African populations, we inferred that the ancestral gene pool of
   P. nigra already harbored polymorphism at position 328 prior to the
   divergence to two lineages currently present in westward and eastward
   parts of the species range distribution. Subsequent occurrence of three
   mutations, which distinguish these two lineages, suggests their
   long-term isolation.",
journal = "Silva Fennica",
title = "Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data",
number = "1415",
volume = "49",
doi = "10.14214/sf.1415"
}

Šarac, Z., Dodos, T., Rajčević, N., Bojović, S., Marin, P. D.,& Aleksić, J. M.. (2015). Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data. in Silva Fennica, 49(1415).
https://doi.org/10.14214/sf.1415

Šarac Z, Dodos T, Rajčević N, Bojović S, Marin PD, Aleksić JM. Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data. in Silva Fennica. 2015;49(1415).
doi:10.14214/sf.1415 .

Šarac, Zorica, Dodos, Tanja, Rajčević, Nemanja, Bojović, Srđan, Marin, Petar D., Aleksić, Jelena M., "Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data" in Silva Fennica, 49, no. 1415 (2015),
https://doi.org/10.14214/sf.1415 . .