TY  - JOUR
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
AU  - Brkljačić, Jelena
AU  - Elaković, Ivana
AU  - Manitašević Jovanović, Sanja
AU  - Elzaedi, Younis Mouftah
AU  - Perisic, Tatjana
AU  - Dunderski, Jadranka
AU  - Damjanovic, Svetozar
AU  - Knezevic, Goran
AU  - Spiric, Zeljko
AU  - Vermetten, Eric
AU  - Savic, Danka
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2244
AB  - Alterations in the number and functional status of mineralocorticoid
   (MR) and glucocorticoid receptors (GR) may contribute to vulnerability
   to posttraumatic stress disorder (PTSD). Corticosteroid receptors are
   chaperoned by heat shock proteins Hsp90 and Hsp70. We examined relations
   between corticosteroid receptor and heat shock protein expression
   levels, and related them with war trauma exposure, PTSD and resilience
   to PTSD. Relative levels of MR. Hsp90 and Hsp70 were determined by
   immunoblotting in lymphocytes from war trauma-exposed men with current
   PTSD (current PTSD group, n=113), with lifetime PTSD (life-time PTSD
   group, n=61) and without PTSD (trauma control group, n=88), and from
   non-traumatized healthy controls (healthy control group, n=85).
   Between-group differences in MR, Hsp90 and Hsp70 levels and in MR/GR
   ratio were not observed. The level of MR was correlated with both Hsp90
   and Hsp70 levels in trauma control and healthy control groups. On the
   other hand, GR level was correlated only with Hsp90 level, and this
   correlation was evident in current PTSD and trauma control groups. In
   conclusion, PTSD and exposure to trauma are not related to changes in
   lymphocyte MR, Hsp90 or Hsp70 levels, but may be associated with
   disturbances in corticosteroid receptors interaction with heat shock
   proteins. (C) 2013 Elsevier Ireland Ltd. All rights reserved.
T2  - Psychiatry Research
T1  - Mineralocorticoid receptor and heat shock protein expression levels in
 peripheral lymphocytes from war trauma-exposed men with and without PTSD
IS  - 2
VL  - 215
DO  - 10.1016/j.psychres.2013.11.022
SP  - 379
EP  - 385
ER  - 

@article{
author = "Matić, Gordana and Vojnović-Milutinović, Danijela and Brkljačić, Jelena and Elaković, Ivana and Manitašević Jovanović, Sanja and Elzaedi, Younis Mouftah and Perisic, Tatjana and Dunderski, Jadranka and Damjanovic, Svetozar and Knezevic, Goran and Spiric, Zeljko and Vermetten, Eric and Savic, Danka",
year = "2014",
abstract = "Alterations in the number and functional status of mineralocorticoid
   (MR) and glucocorticoid receptors (GR) may contribute to vulnerability
   to posttraumatic stress disorder (PTSD). Corticosteroid receptors are
   chaperoned by heat shock proteins Hsp90 and Hsp70. We examined relations
   between corticosteroid receptor and heat shock protein expression
   levels, and related them with war trauma exposure, PTSD and resilience
   to PTSD. Relative levels of MR. Hsp90 and Hsp70 were determined by
   immunoblotting in lymphocytes from war trauma-exposed men with current
   PTSD (current PTSD group, n=113), with lifetime PTSD (life-time PTSD
   group, n=61) and without PTSD (trauma control group, n=88), and from
   non-traumatized healthy controls (healthy control group, n=85).
   Between-group differences in MR, Hsp90 and Hsp70 levels and in MR/GR
   ratio were not observed. The level of MR was correlated with both Hsp90
   and Hsp70 levels in trauma control and healthy control groups. On the
   other hand, GR level was correlated only with Hsp90 level, and this
   correlation was evident in current PTSD and trauma control groups. In
   conclusion, PTSD and exposure to trauma are not related to changes in
   lymphocyte MR, Hsp90 or Hsp70 levels, but may be associated with
   disturbances in corticosteroid receptors interaction with heat shock
   proteins. (C) 2013 Elsevier Ireland Ltd. All rights reserved.",
journal = "Psychiatry Research",
title = "Mineralocorticoid receptor and heat shock protein expression levels in
 peripheral lymphocytes from war trauma-exposed men with and without PTSD",
number = "2",
volume = "215",
doi = "10.1016/j.psychres.2013.11.022",
pages = "379-385"
}

Matić, G., Vojnović-Milutinović, D., Brkljačić, J., Elaković, I., Manitašević Jovanović, S., Elzaedi, Y. M., Perisic, T., Dunderski, J., Damjanovic, S., Knezevic, G., Spiric, Z., Vermetten, E.,& Savic, D.. (2014). Mineralocorticoid receptor and heat shock protein expression levels in
 peripheral lymphocytes from war trauma-exposed men with and without PTSD. in Psychiatry Research, 215(2), 379-385.
https://doi.org/10.1016/j.psychres.2013.11.022

Matić G, Vojnović-Milutinović D, Brkljačić J, Elaković I, Manitašević Jovanović S, Elzaedi YM, Perisic T, Dunderski J, Damjanovic S, Knezevic G, Spiric Z, Vermetten E, Savic D. Mineralocorticoid receptor and heat shock protein expression levels in
 peripheral lymphocytes from war trauma-exposed men with and without PTSD. in Psychiatry Research. 2014;215(2):379-385.
doi:10.1016/j.psychres.2013.11.022 .

Matić, Gordana, Vojnović-Milutinović, Danijela, Brkljačić, Jelena, Elaković, Ivana, Manitašević Jovanović, Sanja, Elzaedi, Younis Mouftah, Perisic, Tatjana, Dunderski, Jadranka, Damjanovic, Svetozar, Knezevic, Goran, Spiric, Zeljko, Vermetten, Eric, Savic, Danka, "Mineralocorticoid receptor and heat shock protein expression levels in
 peripheral lymphocytes from war trauma-exposed men with and without PTSD" in Psychiatry Research, 215, no. 2 (2014):379-385,
https://doi.org/10.1016/j.psychres.2013.11.022 . .

TY  - JOUR
AU  - Matić, Gordana
AU  - Vojnović-Milutinović, Danijela
AU  - Brkljačić, Jelena
AU  - Elaković, Ivana
AU  - Manitašević-Jovanović, Sanja
AU  - Perišić, Tatjana
AU  - Dunđerski, Jadranka S.
AU  - Damjanović, Svetozar S
AU  - Knežević, Goran
AU  - Spirić, Zeljko M
AU  - Vermetten, Eric
AU  - Savić, Danka A
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/999
AB  - Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Progress in Neuro-Psychopharmacology & Biological Psychiatry
T1  - Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD
IS  - null
VL  - 43
SP  - 63
EP  - 245
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_999
ER  - 

@article{
author = "Matić, Gordana and Vojnović-Milutinović, Danijela and Brkljačić, Jelena and Elaković, Ivana and Manitašević-Jovanović, Sanja and Perišić, Tatjana and Dunđerski, Jadranka S. and Damjanović, Svetozar S and Knežević, Goran and Spirić, Zeljko M and Vermetten, Eric and Savić, Danka A",
year = "2013",
abstract = "Objective: Posttraumatic stress disorder (PTSD) has been shown to be associated with altered glucocorticoid receptor (GR) activity. We studied the expression and functional properties of the receptor in peripheral blood mononuclear cells (PBMCs) from non-traumatized healthy individuals (healthy controls; n = 85), and war trauma-exposed individuals with current PTSD (n = 113), with life-time PTSD (n = 61) and without PTSD (trauma controls; n = 88). The aim of the study was to distinguish the receptor alterations related to PTSD from those related to trauma itself or to resilience to PTSD. Methods: Functional status of the receptor was assessed by radioligand binding and lysozyme synthesis inhibition assays. The level of GR gene expression was measured by quantitative PCR and immunoblotting. Results: Current PTSD patients had the lowest, while trauma controls had the highest number of glucocorticoid binding sites (B-max) in PBMCs. Hormone-binding potential (B-max/K-D ratio) of the receptor was diminished in the current PTSD group in comparison to all other study groups. Correlation between B-max and K-D that normally exists in healthy individuals was decreased in the current PTSD group. Contrasting B-max data, GR protein level was lower in trauma controls than in participants with current or life-time PTSD. Conclusions: Current PTSD is characterized by reduced lymphocyte GR hormone-binding potential and by disturbed compensation between B-max and hormone-binding affinity. Resilience to PTSD is associated with enlarged fraction of the receptor molecules capable of hormone binding, within the total receptor molecule population in PBMCs. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Progress in Neuro-Psychopharmacology & Biological Psychiatry",
title = "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD",
number = "null",
volume = "43",
pages = "63-245",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_999"
}

Matić, G., Vojnović-Milutinović, D., Brkljačić, J., Elaković, I., Manitašević-Jovanović, S., Perišić, T., Dunđerski, J. S., Damjanović, S. S., Knežević, G., Spirić, Z. M., Vermetten, E.,& Savić, D. A.. (2013). Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 43(null), 63-245.
https://hdl.handle.net/21.15107/rcub_ibiss_999

Matić G, Vojnović-Milutinović D, Brkljačić J, Elaković I, Manitašević-Jovanović S, Perišić T, Dunđerski JS, Damjanović SS, Knežević G, Spirić ZM, Vermetten E, Savić DA. Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD. in Progress in Neuro-Psychopharmacology & Biological Psychiatry. 2013;43(null):63-245.
https://hdl.handle.net/21.15107/rcub_ibiss_999 .

Matić, Gordana, Vojnović-Milutinović, Danijela, Brkljačić, Jelena, Elaković, Ivana, Manitašević-Jovanović, Sanja, Perišić, Tatjana, Dunđerski, Jadranka S., Damjanović, Svetozar S, Knežević, Goran, Spirić, Zeljko M, Vermetten, Eric, Savić, Danka A, "Lymphocyte glucocorticoid receptor expression level and hormone-binding properties differ between war trauma-exposed men with and without PTSD" in Progress in Neuro-Psychopharmacology & Biological Psychiatry, 43, no. null (2013):63-245,
https://hdl.handle.net/21.15107/rcub_ibiss_999 .