TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Sinan, Kouadio Ibrahime
AU  - Luz Cádiz-Gurrea, María de la
AU  - Javier Leyva-Jiménez, Francisco
AU  - Fernández-Ochoa, Álvaro
AU  - Segura-Carretero, Antonio
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Nenadić, Marija
AU  - Aktumsek, Abdurrahman
AU  - Dall'Acqua, Stefano
AU  - Zengin, Gokhan
PY  - 2021
UR  - http://xlink.rsc.org/?DOI=D1NJ03621A
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4685
AB  - In this paper, the methanol and water extracts of Phyllanthus reticulatus Poir. leaves and stem bark parts were considered as the source of bioactive constituents; thus, chemical analysis and bioactivity assays were performed. UPLC-ESI-QTOF/MS analysis allowed the identification of phytoconstituents in the various extracts, including 19 phenolic acids, 27 flavonoids, 20 tannins and 4 iridoids. The methanol extract of stem bark presented a higher antioxidant activity (DPPH: 3.04 mmol TE g(-1); ABTS: 4.72 mmol TE g(-1); CUPRAC: 3.61 mmol TE g(-1) and FRAP: 1.64 mmol TE g(-1)) compared with other extracts. The highest inhibitory effects (AChE: 2.56 mg GALAE g(-1); tyrosinase: 76.25 mg KAE g(-1); amylase: 0.71 mmol ACAE g(-1); glucosidase: 0.98 mmol ACAE g(-1) and elastase: 2.64 mg CE g(-1)) were also recorded by the methanol extract of stem bark. The bacteriostatic (MIC) and bactericidal (MBC) effects of the extracts obtained from different plant parts and using different solvents were evaluated using clinically relevant microorganisms and the measured values were in the range of the activity measured for the reference compounds streptomycin and ampicillin. Bioinformatics analysis was performed to predict the potential toxicity of the identified compounds as well as potential targets of bioactivity. Five of the major compounds, erythrin, gardenoside, phlorizin, sesamoside and veracylglucan B, were identified as possible modulators of necroptosis pathways. Besides, particular attention must be paid to some identified compounds (i.e. icariin, isomyricitrin, methylmyricetin rhamnoside-gallate, rhapontin, silybin, xyloccensin R, vanilloylajugol) because of their probable immunotoxicity predicted properties. Our results could provide a great scientific starting point on the way from the bench to functional applications through the use of P. reticulatus, namely novel nutraceuticals, pharmaceuticals and cosmeceuticals.
PB  - Cambridge: Royal Society of Chemistry
T2  - New Journal of Chemistry
T1  - New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis
IS  - 45
VL  - 45
DO  - 10.1039/D1NJ03621A
SP  - 21049
EP  - 21065
ER  - 

@article{
author = "Sinan, Kouadio Ibrahime and Luz Cádiz-Gurrea, María de la and Javier Leyva-Jiménez, Francisco and Fernández-Ochoa, Álvaro and Segura-Carretero, Antonio and Glamočlija, Jasmina and Soković, Marina and Nenadić, Marija and Aktumsek, Abdurrahman and Dall'Acqua, Stefano and Zengin, Gokhan",
year = "2021",
abstract = "In this paper, the methanol and water extracts of Phyllanthus reticulatus Poir. leaves and stem bark parts were considered as the source of bioactive constituents; thus, chemical analysis and bioactivity assays were performed. UPLC-ESI-QTOF/MS analysis allowed the identification of phytoconstituents in the various extracts, including 19 phenolic acids, 27 flavonoids, 20 tannins and 4 iridoids. The methanol extract of stem bark presented a higher antioxidant activity (DPPH: 3.04 mmol TE g(-1); ABTS: 4.72 mmol TE g(-1); CUPRAC: 3.61 mmol TE g(-1) and FRAP: 1.64 mmol TE g(-1)) compared with other extracts. The highest inhibitory effects (AChE: 2.56 mg GALAE g(-1); tyrosinase: 76.25 mg KAE g(-1); amylase: 0.71 mmol ACAE g(-1); glucosidase: 0.98 mmol ACAE g(-1) and elastase: 2.64 mg CE g(-1)) were also recorded by the methanol extract of stem bark. The bacteriostatic (MIC) and bactericidal (MBC) effects of the extracts obtained from different plant parts and using different solvents were evaluated using clinically relevant microorganisms and the measured values were in the range of the activity measured for the reference compounds streptomycin and ampicillin. Bioinformatics analysis was performed to predict the potential toxicity of the identified compounds as well as potential targets of bioactivity. Five of the major compounds, erythrin, gardenoside, phlorizin, sesamoside and veracylglucan B, were identified as possible modulators of necroptosis pathways. Besides, particular attention must be paid to some identified compounds (i.e. icariin, isomyricitrin, methylmyricetin rhamnoside-gallate, rhapontin, silybin, xyloccensin R, vanilloylajugol) because of their probable immunotoxicity predicted properties. Our results could provide a great scientific starting point on the way from the bench to functional applications through the use of P. reticulatus, namely novel nutraceuticals, pharmaceuticals and cosmeceuticals.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "New Journal of Chemistry",
title = "New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis",
number = "45",
volume = "45",
doi = "10.1039/D1NJ03621A",
pages = "21049-21065"
}

Sinan, K. I., Luz Cádiz-Gurrea, M. d. l., Javier Leyva-Jiménez, F., Fernández-Ochoa, Á., Segura-Carretero, A., Glamočlija, J., Soković, M., Nenadić, M., Aktumsek, A., Dall'Acqua, S.,& Zengin, G.. (2021). New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis. in New Journal of Chemistry
Cambridge: Royal Society of Chemistry., 45(45), 21049-21065.
https://doi.org/10.1039/D1NJ03621A

Sinan KI, Luz Cádiz-Gurrea MDL, Javier Leyva-Jiménez F, Fernández-Ochoa Á, Segura-Carretero A, Glamočlija J, Soković M, Nenadić M, Aktumsek A, Dall'Acqua S, Zengin G. New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis. in New Journal of Chemistry. 2021;45(45):21049-21065.
doi:10.1039/D1NJ03621A .

Sinan, Kouadio Ibrahime, Luz Cádiz-Gurrea, María de la, Javier Leyva-Jiménez, Francisco, Fernández-Ochoa, Álvaro, Segura-Carretero, Antonio, Glamočlija, Jasmina, Soković, Marina, Nenadić, Marija, Aktumsek, Abdurrahman, Dall'Acqua, Stefano, Zengin, Gokhan, "New insights on Phyllanthus reticulatus Poir. leaves and stem bark extracts: UPLC-ESI-TOF-MS profiles, and biopharmaceutical and in silico analysis" in New Journal of Chemistry, 45, no. 45 (2021):21049-21065,
https://doi.org/10.1039/D1NJ03621A . .

TY  - JOUR
AU  - Dimkić, Ivica
AU  - Fira, Đorđe
AU  - Janakiev, Tamara
AU  - Kabić, Jovana
AU  - Stupar, Miloš
AU  - Nenadić, Marija
AU  - Unković, Nikola
AU  - Ljaljević Grbić, Milica
PY  - 2021
UR  - internal-pdf://Dimkić et al. - 2021 - The microbiome of bat guano for what is this knowledge important.pdf
UR  - https://doi.org/10.1007/s00253-021-11143-y
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4140
AB  - Abstract: Bats as flying mammals are potent vectors and natural reservoir hosts for many infectious viruses, bacteria, and fungi, also detected in their excreta such as guano. Accelerated deforestation, urbanization, and anthropization hastily lead to overpopulation of the bats in urban areas allowing easy interaction with other animals, expansion, and emergence of new zoonotic disease outbreaks potentially harmful to humans. Therefore, getting new insights in the microbiome of bat guano from different places represents an imperative for the future. Furthermore, the use of novel high-throughput sequencing technologies allows better insight in guano microbiome and potentially indicated that some species could be typical guano-dwelling members. Bats are well known as a natural reservoir of many zoonotic viruses such as Ebola, Nipah, Marburg, lyssaviruses, rabies, henipaviruses, and many coronaviruses which caused a high number of outbreaks including ongoing COVID-19 pandemic. Additionally, many bacterial and fungal pathogens were identified as common guano residents. Thus, the presence of multi-drug-resistant bacteria as environmental reservoirs of extended spectrum β-lactamases and carbapenemase-producing strains has been confirmed. Bat guano is the most suitable substrate for fungal reproduction and dissemination, including pathogenic yeasts and keratinophilic and dimorphic human pathogenic fungi known as notorious causative agents of severe endemic mycoses like histoplasmosis and fatal cryptococcosis, especially deadly in immunocompromised individuals. This review provides an overview of bat guano microbiota diversity and the significance of autochthonous and pathogenic taxa for humans and the environment, highlighting better understanding in preventing emerging diseases. Key points: Bat guano as reservoir and source for spreading of autochthonous and pathogenic microbiotaBat guano vs. novel zoonotic disease outbreaksDestruction of bat natural habitats urgently demands increased human awareness.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Applied Microbiology and Biotechnology
T1  - The microbiome of bat guano: for what is this knowledge important?
IS  - 4
VL  - 105
DO  - 10.1007/s00253-021-11143-y
SP  - 1407
EP  - 1419
ER  - 

@article{
author = "Dimkić, Ivica and Fira, Đorđe and Janakiev, Tamara and Kabić, Jovana and Stupar, Miloš and Nenadić, Marija and Unković, Nikola and Ljaljević Grbić, Milica",
year = "2021",
abstract = "Abstract: Bats as flying mammals are potent vectors and natural reservoir hosts for many infectious viruses, bacteria, and fungi, also detected in their excreta such as guano. Accelerated deforestation, urbanization, and anthropization hastily lead to overpopulation of the bats in urban areas allowing easy interaction with other animals, expansion, and emergence of new zoonotic disease outbreaks potentially harmful to humans. Therefore, getting new insights in the microbiome of bat guano from different places represents an imperative for the future. Furthermore, the use of novel high-throughput sequencing technologies allows better insight in guano microbiome and potentially indicated that some species could be typical guano-dwelling members. Bats are well known as a natural reservoir of many zoonotic viruses such as Ebola, Nipah, Marburg, lyssaviruses, rabies, henipaviruses, and many coronaviruses which caused a high number of outbreaks including ongoing COVID-19 pandemic. Additionally, many bacterial and fungal pathogens were identified as common guano residents. Thus, the presence of multi-drug-resistant bacteria as environmental reservoirs of extended spectrum β-lactamases and carbapenemase-producing strains has been confirmed. Bat guano is the most suitable substrate for fungal reproduction and dissemination, including pathogenic yeasts and keratinophilic and dimorphic human pathogenic fungi known as notorious causative agents of severe endemic mycoses like histoplasmosis and fatal cryptococcosis, especially deadly in immunocompromised individuals. This review provides an overview of bat guano microbiota diversity and the significance of autochthonous and pathogenic taxa for humans and the environment, highlighting better understanding in preventing emerging diseases. Key points: Bat guano as reservoir and source for spreading of autochthonous and pathogenic microbiotaBat guano vs. novel zoonotic disease outbreaksDestruction of bat natural habitats urgently demands increased human awareness.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Applied Microbiology and Biotechnology",
title = "The microbiome of bat guano: for what is this knowledge important?",
number = "4",
volume = "105",
doi = "10.1007/s00253-021-11143-y",
pages = "1407-1419"
}

Dimkić, I., Fira, Đ., Janakiev, T., Kabić, J., Stupar, M., Nenadić, M., Unković, N.,& Ljaljević Grbić, M.. (2021). The microbiome of bat guano: for what is this knowledge important?. in Applied Microbiology and Biotechnology
Springer Science and Business Media Deutschland GmbH., 105(4), 1407-1419.
https://doi.org/10.1007/s00253-021-11143-y

Dimkić I, Fira Đ, Janakiev T, Kabić J, Stupar M, Nenadić M, Unković N, Ljaljević Grbić M. The microbiome of bat guano: for what is this knowledge important?. in Applied Microbiology and Biotechnology. 2021;105(4):1407-1419.
doi:10.1007/s00253-021-11143-y .

Dimkić, Ivica, Fira, Đorđe, Janakiev, Tamara, Kabić, Jovana, Stupar, Miloš, Nenadić, Marija, Unković, Nikola, Ljaljević Grbić, Milica, "The microbiome of bat guano: for what is this knowledge important?" in Applied Microbiology and Biotechnology, 105, no. 4 (2021):1407-1419,
https://doi.org/10.1007/s00253-021-11143-y . .

TY  - CONF
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4368
AB  - In beetles, secretions are products of different exocrine glands, of which particular 
emphasis is on pygidial glands, which are common for the suborder Adephaga. 
Antimicrobial activity of the pygidial gland secretions of six selected ground beetle 
species was observed against human pathogens. A microdilution method was applied 
in order to determine minimum inhibitory concentrations (MICs), minimum bactericidal 
concentrations (MBCs) and minimum fungicidal concentrations (MFCs). We tested 
16 laboratory and clinical strains of human pathogens (eight bacterial gram-positive 
and gram-negative species, and eight fungal species). The secretions of tested ground 
beetles have a certain level of antimicrobial activity, which differed between species, 
which is caused by the difference in chemical composition of the mixtures. The highest 
antibacterial effect was observed after treatment of most pathogens with secretion of 
Laemostenus punctatus and Carabus ullrichii. The highest resistance of tested pathogenic 
strains was noticed after treatment of the secretion of Calosoma sycophanta, which 
achieved antibacterial effect against E. coli only. The secretions of L. punctatus and C. 
sycophanta each showed a strong antifungal effect compared to positive controls. 
Inhibition of the proliferation of human tumor cell lines and porcine non-tumor 
cells by the secretion of adults of four ground beetle species was observed as well. 
The sulphorhodamine B (SRB) assay was applied to establish GI50 values of the tested 
secretions. All secretions have shown certain antiproliferative effect on the tested 
cell lines, but statistically insignificant in most cases, which confirms the absence of 
cytotoxicity on the non-tumor cell line. Special emphasis is put on the secretion of L. 
punctatus, which reached approximately 50% inhibition of cell proliferation (GI50 value) 
of MCF7 (breast adenocarcinoma) cell line, and showed a potential antitumour activity. 
The antimicrobial, antitumour and antiproliferative potential of the secretions of ground 
beetles was demonstrated for the first time in this study.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
T1  - The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products
SP  - 43
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4368
ER  - 

@conference{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Tešević, Vele and Vujisić, Ljubodrag and Vesović, Nikola and Ćurčić, Srećko",
year = "2019",
abstract = "In beetles, secretions are products of different exocrine glands, of which particular 
emphasis is on pygidial glands, which are common for the suborder Adephaga. 
Antimicrobial activity of the pygidial gland secretions of six selected ground beetle 
species was observed against human pathogens. A microdilution method was applied 
in order to determine minimum inhibitory concentrations (MICs), minimum bactericidal 
concentrations (MBCs) and minimum fungicidal concentrations (MFCs). We tested 
16 laboratory and clinical strains of human pathogens (eight bacterial gram-positive 
and gram-negative species, and eight fungal species). The secretions of tested ground 
beetles have a certain level of antimicrobial activity, which differed between species, 
which is caused by the difference in chemical composition of the mixtures. The highest 
antibacterial effect was observed after treatment of most pathogens with secretion of 
Laemostenus punctatus and Carabus ullrichii. The highest resistance of tested pathogenic 
strains was noticed after treatment of the secretion of Calosoma sycophanta, which 
achieved antibacterial effect against E. coli only. The secretions of L. punctatus and C. 
sycophanta each showed a strong antifungal effect compared to positive controls. 
Inhibition of the proliferation of human tumor cell lines and porcine non-tumor 
cells by the secretion of adults of four ground beetle species was observed as well. 
The sulphorhodamine B (SRB) assay was applied to establish GI50 values of the tested 
secretions. All secretions have shown certain antiproliferative effect on the tested 
cell lines, but statistically insignificant in most cases, which confirms the absence of 
cytotoxicity on the non-tumor cell line. Special emphasis is put on the secretion of L. 
punctatus, which reached approximately 50% inhibition of cell proliferation (GI50 value) 
of MCF7 (breast adenocarcinoma) cell line, and showed a potential antitumour activity. 
The antimicrobial, antitumour and antiproliferative potential of the secretions of ground 
beetles was demonstrated for the first time in this study.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia",
title = "The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products",
pages = "43",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4368"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Tešević, V., Vujisić, L., Vesović, N.,& Ćurčić, S.. (2019). The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 43.
https://hdl.handle.net/21.15107/rcub_ibiss_4368

Nenadić M, Soković M, Glamočlija J, Ćirić A, Tešević V, Vujisić L, Vesović N, Ćurčić S. The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products. in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia. 2019;:43.
https://hdl.handle.net/21.15107/rcub_ibiss_4368 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Tešević, Vele, Vujisić, Ljubodrag, Vesović, Nikola, Ćurčić, Srećko, "The pygidial gland secretions of ground beetles (Insecta:  Coleoptera: Carabidae): antimicrobial and antitumour activity of  the natural products" in The 2nd Balkans-China Mini-symposium on Natural Products and Drug Discovery; 2019 Apr 11-13; Belgrade, Serbia (2019):43,
https://hdl.handle.net/21.15107/rcub_ibiss_4368 .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Ljaljević-Grbić, Milica
AU  - Stupar, Miloš
AU  - Vukojević, Jelena
AU  - Ćirić, Ana
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Vesović, Nikola
AU  - Živković, Nemanja
AU  - Ćurčić, Srećko
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4296
AB  - The antifungal potential of the pygidial gland secretion of the troglophilic ground beetle Laemostenus
punctatus from a cave in Southeastern Serbia against cavedwelling micromycetes, isolated from the same habitat, has
been investigated. Eleven collected samples were analyzed
and 32 isolates of cave-dwelling fungi were documented. A
total of 14 fungal species were identified as members of the
genera Aspergillus, Penicillium, Alternaria, Cladosporium,
Rhizopus, Trichoderma, Arthrinium, Aureobasidium,
Epicoccum, Talaromyces, and Fusarium. Five isolates were
selected for testing the antifungal activity of the pygidial gland
se cr etio n: Talaromyces d uclauxi, Aspergillus
brunneouniseriatus, Penicillium sp., Rhizopus stolonifer, and
Trichoderma viride. The microdilution method has been applied to detect minimal inhibitory concentrations (MICs) and
minimal fungicidal concentrations (MFCs). The most sensitive isolate was Penicillium sp., while the other isolates demonstrated a high level of resistance to the tested agent.
L. punctatus has developed a special mechanism of producing
specific compounds that act synergistically within the
secretion mixture, which are responsible for the antifungal
action against pathogens from the cave. The results open opportunities for further research in the field of ground beetle
defense against pathogens, which could have an important
application in human medicine, in addition to the environmental impact, primarily.
PB  - Berlin, Heidelberg: Springer-Verlag
T2  - The Science of Nature
T1  - Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes
IS  - 5-6
VL  - 104
DO  - 10.1007/s00114-017-1474-4
SP  - 52
ER  - 

@article{
author = "Nenadić, Marija and Ljaljević-Grbić, Milica and Stupar, Miloš and Vukojević, Jelena and Ćirić, Ana and Tešević, Vele and Vujisić, Ljubodrag and Todosijević, Marina and Vesović, Nikola and Živković, Nemanja and Ćurčić, Srećko",
year = "2017",
abstract = "The antifungal potential of the pygidial gland secretion of the troglophilic ground beetle Laemostenus
punctatus from a cave in Southeastern Serbia against cavedwelling micromycetes, isolated from the same habitat, has
been investigated. Eleven collected samples were analyzed
and 32 isolates of cave-dwelling fungi were documented. A
total of 14 fungal species were identified as members of the
genera Aspergillus, Penicillium, Alternaria, Cladosporium,
Rhizopus, Trichoderma, Arthrinium, Aureobasidium,
Epicoccum, Talaromyces, and Fusarium. Five isolates were
selected for testing the antifungal activity of the pygidial gland
se cr etio n: Talaromyces d uclauxi, Aspergillus
brunneouniseriatus, Penicillium sp., Rhizopus stolonifer, and
Trichoderma viride. The microdilution method has been applied to detect minimal inhibitory concentrations (MICs) and
minimal fungicidal concentrations (MFCs). The most sensitive isolate was Penicillium sp., while the other isolates demonstrated a high level of resistance to the tested agent.
L. punctatus has developed a special mechanism of producing
specific compounds that act synergistically within the
secretion mixture, which are responsible for the antifungal
action against pathogens from the cave. The results open opportunities for further research in the field of ground beetle
defense against pathogens, which could have an important
application in human medicine, in addition to the environmental impact, primarily.",
publisher = "Berlin, Heidelberg: Springer-Verlag",
journal = "The Science of Nature",
title = "Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes",
number = "5-6",
volume = "104",
doi = "10.1007/s00114-017-1474-4",
pages = "52"
}

Nenadić, M., Ljaljević-Grbić, M., Stupar, M., Vukojević, J., Ćirić, A., Tešević, V., Vujisić, L., Todosijević, M., Vesović, N., Živković, N.,& Ćurčić, S.. (2017). Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes. in The Science of Nature
Berlin, Heidelberg: Springer-Verlag., 104(5-6), 52.
https://doi.org/10.1007/s00114-017-1474-4

Nenadić M, Ljaljević-Grbić M, Stupar M, Vukojević J, Ćirić A, Tešević V, Vujisić L, Todosijević M, Vesović N, Živković N, Ćurčić S. Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes. in The Science of Nature. 2017;104(5-6):52.
doi:10.1007/s00114-017-1474-4 .

Nenadić, Marija, Ljaljević-Grbić, Milica, Stupar, Miloš, Vukojević, Jelena, Ćirić, Ana, Tešević, Vele, Vujisić, Ljubodrag, Todosijević, Marina, Vesović, Nikola, Živković, Nemanja, Ćurčić, Srećko, "Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes" in The Science of Nature, 104, no. 5-6 (2017):52,
https://doi.org/10.1007/s00114-017-1474-4 . .

TY  - JOUR
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
AU  - Vujisić, Ljubodrag
AU  - Nenadić, Marija
AU  - Krstić, Gordana
AU  - Perić Mataruga, Vesna
AU  - Milosavljević, Slobodan
AU  - Antić, Dragan
AU  - Mandić, Boris
AU  - Petković, Matija
AU  - Vučković, Ivan
AU  - Marković, Đorđe
AU  - Vrbica, Maja
AU  - Ćurčić, Božidar
AU  - Makarov, Slobodan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1947
AB  - Three adult cave-dwelling ground beetle species were induced to
   discharge secretions of their pygidial glands into vials.
   Dichloromethane extraction was used to obtain the secretions. In total,
   42 compounds were identified by GC/MS analysis. Pheggomisetes ninae
   contained 32 glandular compounds, Laemostenus (Pristonychus) punctatus
   13, whereas Duvalius (Paraduvalius) milutini had nine compounds.
   Caproic, oleic, palmitic, and stearic acids were present in the samples
   of all analyzed species. Undecane was predominant in the extract of L.
   punctatus. Palmitic acid was the major component in the secretion of D.
   milutini. Finally, the most abundant compounds in P. ninae secretion
   were heptacosene and nonacosadienes. Herein, we present the first data
   on the identification of pygidial gland secretion components in both
   troglophilous and troglobite cave-dwelling ground beetles. Some
   compounds are reported for the first time in the secretions of ground
   beetles and other higher or lower taxa. The adaptation to underground
   life has not led to a reduction or changes in the chemical defense
   mechanism in the analyzed troglophilous and troglobitic Platyninae and
   Trechinae taxa.
PB  - Springer New York LLC
T2  - Journal of Chemical Ecology
T1  - Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence
IS  - 6
VL  - 41
DO  - 10.1007/s10886-015-0593-7
SP  - 533
EP  - 539
ER  - 

@article{
author = "Vesović, Nikola and Ćurčić, Srećko and Vujisić, Ljubodrag and Nenadić, Marija and Krstić, Gordana and Perić Mataruga, Vesna and Milosavljević, Slobodan and Antić, Dragan and Mandić, Boris and Petković, Matija and Vučković, Ivan and Marković, Đorđe and Vrbica, Maja and Ćurčić, Božidar and Makarov, Slobodan",
year = "2015",
abstract = "Three adult cave-dwelling ground beetle species were induced to
   discharge secretions of their pygidial glands into vials.
   Dichloromethane extraction was used to obtain the secretions. In total,
   42 compounds were identified by GC/MS analysis. Pheggomisetes ninae
   contained 32 glandular compounds, Laemostenus (Pristonychus) punctatus
   13, whereas Duvalius (Paraduvalius) milutini had nine compounds.
   Caproic, oleic, palmitic, and stearic acids were present in the samples
   of all analyzed species. Undecane was predominant in the extract of L.
   punctatus. Palmitic acid was the major component in the secretion of D.
   milutini. Finally, the most abundant compounds in P. ninae secretion
   were heptacosene and nonacosadienes. Herein, we present the first data
   on the identification of pygidial gland secretion components in both
   troglophilous and troglobite cave-dwelling ground beetles. Some
   compounds are reported for the first time in the secretions of ground
   beetles and other higher or lower taxa. The adaptation to underground
   life has not led to a reduction or changes in the chemical defense
   mechanism in the analyzed troglophilous and troglobitic Platyninae and
   Trechinae taxa.",
publisher = "Springer New York LLC",
journal = "Journal of Chemical Ecology",
title = "Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence",
number = "6",
volume = "41",
doi = "10.1007/s10886-015-0593-7",
pages = "533-539"
}

Vesović, N., Ćurčić, S., Vujisić, L., Nenadić, M., Krstić, G., Perić Mataruga, V., Milosavljević, S., Antić, D., Mandić, B., Petković, M., Vučković, I., Marković, Đ., Vrbica, M., Ćurčić, B.,& Makarov, S.. (2015). Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence. in Journal of Chemical Ecology
Springer New York LLC., 41(6), 533-539.
https://doi.org/10.1007/s10886-015-0593-7

Vesović N, Ćurčić S, Vujisić L, Nenadić M, Krstić G, Perić Mataruga V, Milosavljević S, Antić D, Mandić B, Petković M, Vučković I, Marković Đ, Vrbica M, Ćurčić B, Makarov S. Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence. in Journal of Chemical Ecology. 2015;41(6):533-539.
doi:10.1007/s10886-015-0593-7 .

Vesović, Nikola, Ćurčić, Srećko, Vujisić, Ljubodrag, Nenadić, Marija, Krstić, Gordana, Perić Mataruga, Vesna, Milosavljević, Slobodan, Antić, Dragan, Mandić, Boris, Petković, Matija, Vučković, Ivan, Marković, Đorđe, Vrbica, Maja, Ćurčić, Božidar, Makarov, Slobodan, "Molecular Diversity of Compounds from Pygidial Gland Secretions of
 Cave-Dwelling Ground Beetles: The First Evidence" in Journal of Chemical Ecology, 41, no. 6 (2015):533-539,
https://doi.org/10.1007/s10886-015-0593-7 . .