TY  - JOUR
AU  - Babotă, Mihai
AU  - Frumuzachi, Oleg
AU  - Nicolescu, Alexandru
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Rocchetti, Gabriele
AU  - Zhang, Leilei
AU  - Lucini, Luigi
AU  - Crișan, Gianina
AU  - Mocan, Andrei
AU  - Vostinaru, Oliviu
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5502
AB  - Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species.
PB  - Frontiers Media S.A.
T2  - Frontiers in Pharmacology
T1  - Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
VL  - 14
DO  - 10.3389/fphar.2023.1115117
SP  - 1115117
ER  - 

@article{
author = "Babotă, Mihai and Frumuzachi, Oleg and Nicolescu, Alexandru and Stojković, Dejan and Soković, Marina and Rocchetti, Gabriele and Zhang, Leilei and Lucini, Luigi and Crișan, Gianina and Mocan, Andrei and Vostinaru, Oliviu",
year = "2023",
abstract = "Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Pharmacology",
title = "Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania",
volume = "14",
doi = "10.3389/fphar.2023.1115117",
pages = "1115117"
}

Babotă, M., Frumuzachi, O., Nicolescu, A., Stojković, D., Soković, M., Rocchetti, G., Zhang, L., Lucini, L., Crișan, G., Mocan, A.,& Vostinaru, O.. (2023). Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania. in Frontiers in Pharmacology
Frontiers Media S.A.., 14, 1115117.
https://doi.org/10.3389/fphar.2023.1115117

Babotă M, Frumuzachi O, Nicolescu A, Stojković D, Soković M, Rocchetti G, Zhang L, Lucini L, Crișan G, Mocan A, Vostinaru O. Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania. in Frontiers in Pharmacology. 2023;14:1115117.
doi:10.3389/fphar.2023.1115117 .

Babotă, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Stojković, Dejan, Soković, Marina, Rocchetti, Gabriele, Zhang, Leilei, Lucini, Luigi, Crișan, Gianina, Mocan, Andrei, Vostinaru, Oliviu, "Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania" in Frontiers in Pharmacology, 14 (2023):1115117,
https://doi.org/10.3389/fphar.2023.1115117 . .

TY  - JOUR
AU  - Senizza, Biancamaria
AU  - Rocchetti, Gabriele
AU  - Sinan, Kouadio Ibrahime
AU  - Zengin, Gokhan
AU  - Mahomoodally, Mohamad Fawzi
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Etienne, Ouattara Katinan
AU  - Lucini, Luigi
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4171
AB  - Solanum erianthum D. Don. and S. torvum Swartz of the family Solanaceae have been extensively used for both food and pharmaceutical purposes. In this study, a metabolomic approach was used to compare the phytochemical composition and biological activity of the different plant parts of both species. The inhibitory activity against important enzymes (i.e., cholinesterases, tyrosinase, α-amylase and α-glucosidase), antimicrobial and antifungal potentials, in vitro antioxidant activity, as well as the phenolic and alkaloid profiles of the fruit, leaf and stem bark extracts of both species were studied. The results showed that the stem barks were characterized by the highest amounts of total phenolics (on average: 12.6 mg/g), whilst the S. erianthum leaves were the best source of total alkaloids (2.1 mg/g). Overall, the stem bark extracts of both species were found to be a good source of antioxidant compounds. All the extracts were effective against acetylcholinesterase and tyrosinase, while only the leaf extracts of both plants were a good source of butyrylcholinesterase inhibitors. The extracts have significant anti-microbial and anti-fungal potentials. A modest anti-diabetic effect was measured for α-amylase and α-glucosidase. Strong correlations (p <0.01) were found between the bioactives and the biological activities. Therefore, this study showed the potential of untargeted metabolomics to investigate the bioactive profile of plant extracts, thus providing discrimination between S. erianthum and S. torvum.
PB  - Elsevier Ltd
T2  - Food Bioscience
T1  - The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties
VL  - 41
DO  - 10.1016/j.fbio.2021.100974
SP  - 100974
ER  - 

@article{
author = "Senizza, Biancamaria and Rocchetti, Gabriele and Sinan, Kouadio Ibrahime and Zengin, Gokhan and Mahomoodally, Mohamad Fawzi and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Etienne, Ouattara Katinan and Lucini, Luigi",
year = "2021",
abstract = "Solanum erianthum D. Don. and S. torvum Swartz of the family Solanaceae have been extensively used for both food and pharmaceutical purposes. In this study, a metabolomic approach was used to compare the phytochemical composition and biological activity of the different plant parts of both species. The inhibitory activity against important enzymes (i.e., cholinesterases, tyrosinase, α-amylase and α-glucosidase), antimicrobial and antifungal potentials, in vitro antioxidant activity, as well as the phenolic and alkaloid profiles of the fruit, leaf and stem bark extracts of both species were studied. The results showed that the stem barks were characterized by the highest amounts of total phenolics (on average: 12.6 mg/g), whilst the S. erianthum leaves were the best source of total alkaloids (2.1 mg/g). Overall, the stem bark extracts of both species were found to be a good source of antioxidant compounds. All the extracts were effective against acetylcholinesterase and tyrosinase, while only the leaf extracts of both plants were a good source of butyrylcholinesterase inhibitors. The extracts have significant anti-microbial and anti-fungal potentials. A modest anti-diabetic effect was measured for α-amylase and α-glucosidase. Strong correlations (p <0.01) were found between the bioactives and the biological activities. Therefore, this study showed the potential of untargeted metabolomics to investigate the bioactive profile of plant extracts, thus providing discrimination between S. erianthum and S. torvum.",
publisher = "Elsevier Ltd",
journal = "Food Bioscience",
title = "The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties",
volume = "41",
doi = "10.1016/j.fbio.2021.100974",
pages = "100974"
}

Senizza, B., Rocchetti, G., Sinan, K. I., Zengin, G., Mahomoodally, M. F., Glamočlija, J., Soković, M., Lobine, D., Etienne, O. K.,& Lucini, L.. (2021). The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties. in Food Bioscience
Elsevier Ltd., 41, 100974.
https://doi.org/10.1016/j.fbio.2021.100974

Senizza B, Rocchetti G, Sinan KI, Zengin G, Mahomoodally MF, Glamočlija J, Soković M, Lobine D, Etienne OK, Lucini L. The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties. in Food Bioscience. 2021;41:100974.
doi:10.1016/j.fbio.2021.100974 .

Senizza, Biancamaria, Rocchetti, Gabriele, Sinan, Kouadio Ibrahime, Zengin, Gokhan, Mahomoodally, Mohamad Fawzi, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Etienne, Ouattara Katinan, Lucini, Luigi, "The phenolic and alkaloid profiles of Solanum erianthum and Solanum torvum modulated their biological properties" in Food Bioscience, 41 (2021):100974,
https://doi.org/10.1016/j.fbio.2021.100974 . .

TY  - JOUR
AU  - Rocchetti, Gabriele
AU  - Zengin, Gokhan
AU  - Cakmak, Yavuz Selim
AU  - Mahomoodally, Mohamad Fawzi
AU  - Kaya, Muhammed Fettah
AU  - Alsheikh, Sarah Mohammed
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Lobine, Devina
AU  - Lucini, Luigi
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/123456789/3907
AB  - The genus Consolida is one of the most important within the Ranunculaceae family and several members of this genus contain important biologically active compounds, such as phenolics and alkaloids. The present study attempts for the first time to assess the biological properties and phytochemical constituents of six Consolida species (C. glandulosa (Boiss. & A. Huet) Bornm, C. hellospontica (Boiss.) Chater, C. raveyi (Boiss.) Schrödinger, C. regalis (Boiss.) Schrödinger, C. staminosa P.H. Davis & Sorger and C. stenocarpa (P.H. Davis & M. Hossain) P.H. Davis) growing in Turkey. A comprehensive phytochemical profiling of the different species was achieved by using an ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) mass spectrometry, targeting polyphenols and diterpene alkaloids. Also, the in vitro antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), metal chelating and phosphomolybdenum) and enzyme inhibition (cholinesterases, tyrosinase, α-amylase, and α-glucosidase) potentials of the different Consolida methanolic extracts were evaluated. The UHPLC-QTOF profiling revealed 492 compounds, being 236 flavonoids, 93 phenolic acids, 78 tyrosol derivatives, 49 diterpene alkaloids, 29 lignans, and 7 stilbenes. The cumulative phenolic content ranged from 17.65 mg Eq./g for C. staminosa up to 43.04 mg Eq./g for C. glandulosa, whilst the total diterpene alkaloids content was exclusively found in C. glandulosa extracts (3.53 mg Eq./g). Consolida regalis (58. 61 mg trolox equivalent (TE)/g for DPPH and 65.38 mg TE/g for ABTS) and C. stenocarpa (46.81 mg TE/g for DPPH and 78.12 mg TE/g for ABTS) exhibited the highest anti-radical abilities, while the best reducing power values were recorded for C. glandulosa (129.46 mg TE/g for CUPRAC and 77.01 mg TE/g for FRAP) and C. raveyi (124.56 mg TE/g for CUPRAC and 78.36 mg TE/g for FRAP). Also, following the cholinesterases inhibition assays, C. hellospontica and C. glandulosa exhibited the highest inhibitory effects. Regarding tyrosinase inhibitory effects, C. raveyi showed the highest value, being 126.60 mg kojic acid equivalent (KAE)/g, followed by C. glandulosa (125.82 mg KAE/g) and C. stenocarpa (123.09 mg KAE/g). Besides, Consolida staminosa showed potential inhibition against α-amylase, but low glucosidase inhibitory properties. Therefore, the present findings could provide a starting point for further investigations to promote the industrial exploitation of these species with the aim of designing novel phyto-pharmaceuticals.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey
VL  - 158
DO  - 10.1016/j.indcrop.2020.112966
SP  - 112966
ER  - 

@article{
author = "Rocchetti, Gabriele and Zengin, Gokhan and Cakmak, Yavuz Selim and Mahomoodally, Mohamad Fawzi and Kaya, Muhammed Fettah and Alsheikh, Sarah Mohammed and Glamočlija, Jasmina and Soković, Marina and Lobine, Devina and Lucini, Luigi",
year = "2020",
abstract = "The genus Consolida is one of the most important within the Ranunculaceae family and several members of this genus contain important biologically active compounds, such as phenolics and alkaloids. The present study attempts for the first time to assess the biological properties and phytochemical constituents of six Consolida species (C. glandulosa (Boiss. & A. Huet) Bornm, C. hellospontica (Boiss.) Chater, C. raveyi (Boiss.) Schrödinger, C. regalis (Boiss.) Schrödinger, C. staminosa P.H. Davis & Sorger and C. stenocarpa (P.H. Davis & M. Hossain) P.H. Davis) growing in Turkey. A comprehensive phytochemical profiling of the different species was achieved by using an ultra-high-performance liquid chromatography-quadrupole time-of-flight (UHPLC-QTOF) mass spectrometry, targeting polyphenols and diterpene alkaloids. Also, the in vitro antioxidant assays (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), ferric reducing antioxidant power (FRAP), cupric reducing antioxidant capacity (CUPRAC), metal chelating and phosphomolybdenum) and enzyme inhibition (cholinesterases, tyrosinase, α-amylase, and α-glucosidase) potentials of the different Consolida methanolic extracts were evaluated. The UHPLC-QTOF profiling revealed 492 compounds, being 236 flavonoids, 93 phenolic acids, 78 tyrosol derivatives, 49 diterpene alkaloids, 29 lignans, and 7 stilbenes. The cumulative phenolic content ranged from 17.65 mg Eq./g for C. staminosa up to 43.04 mg Eq./g for C. glandulosa, whilst the total diterpene alkaloids content was exclusively found in C. glandulosa extracts (3.53 mg Eq./g). Consolida regalis (58. 61 mg trolox equivalent (TE)/g for DPPH and 65.38 mg TE/g for ABTS) and C. stenocarpa (46.81 mg TE/g for DPPH and 78.12 mg TE/g for ABTS) exhibited the highest anti-radical abilities, while the best reducing power values were recorded for C. glandulosa (129.46 mg TE/g for CUPRAC and 77.01 mg TE/g for FRAP) and C. raveyi (124.56 mg TE/g for CUPRAC and 78.36 mg TE/g for FRAP). Also, following the cholinesterases inhibition assays, C. hellospontica and C. glandulosa exhibited the highest inhibitory effects. Regarding tyrosinase inhibitory effects, C. raveyi showed the highest value, being 126.60 mg kojic acid equivalent (KAE)/g, followed by C. glandulosa (125.82 mg KAE/g) and C. stenocarpa (123.09 mg KAE/g). Besides, Consolida staminosa showed potential inhibition against α-amylase, but low glucosidase inhibitory properties. Therefore, the present findings could provide a starting point for further investigations to promote the industrial exploitation of these species with the aim of designing novel phyto-pharmaceuticals.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey",
volume = "158",
doi = "10.1016/j.indcrop.2020.112966",
pages = "112966"
}

Rocchetti, G., Zengin, G., Cakmak, Y. S., Mahomoodally, M. F., Kaya, M. F., Alsheikh, S. M., Glamočlija, J., Soković, M., Lobine, D.,& Lucini, L.. (2020). A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey. in Industrial Crops and Products
Elsevier B.V.., 158, 112966.
https://doi.org/10.1016/j.indcrop.2020.112966

Rocchetti G, Zengin G, Cakmak YS, Mahomoodally MF, Kaya MF, Alsheikh SM, Glamočlija J, Soković M, Lobine D, Lucini L. A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey. in Industrial Crops and Products. 2020;158:112966.
doi:10.1016/j.indcrop.2020.112966 .

Rocchetti, Gabriele, Zengin, Gokhan, Cakmak, Yavuz Selim, Mahomoodally, Mohamad Fawzi, Kaya, Muhammed Fettah, Alsheikh, Sarah Mohammed, Glamočlija, Jasmina, Soković, Marina, Lobine, Devina, Lucini, Luigi, "A UHPLC-QTOF-MS screening provides new insights into the phytochemical composition and biological properties of six Consolida species from Turkey" in Industrial Crops and Products, 158 (2020):112966,
https://doi.org/10.1016/j.indcrop.2020.112966 . .