TY  - JOUR
AU  - Matić Petrović, Sanja
AU  - Nikolić, Nađa
AU  - Toljić, Boško
AU  - Arambašić Jovanović, Jelena
AU  - Miličić, Biljana
AU  - Miličić, Tanja
AU  - Jotić, Aleksandra
AU  - Vidaković, Melita
AU  - Milašin, Jelena
AU  - Pucar, Ana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3945
AB  - Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between −308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed. Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA). Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037−0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35−7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954−0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D. Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.
PB  - Elsevier Ltd
T2  - Archives of Oral Biology
T1  - The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population
VL  - 120
DO  - 10.1016/j.archoralbio.2020.104929
SP  - 104929
ER  - 

@article{
author = "Matić Petrović, Sanja and Nikolić, Nađa and Toljić, Boško and Arambašić Jovanović, Jelena and Miličić, Biljana and Miličić, Tanja and Jotić, Aleksandra and Vidaković, Melita and Milašin, Jelena and Pucar, Ana",
year = "2020",
abstract = "Objectives: Aiming to show that periodontitis (PD) and type 2 diabetes (T2D) are bidirectionally related and potentially linked by inflammatory cytokines, we searched for association between −308 G/A Tumor necrosis factor-alpha (TNFα), +252A/G Lymphotoxin-alpha (LTα), +36A/G Tumor necrosis factor receptor 1 (TNFR1) and +676 T/G tumor necrosis factor receptor 2 (TNFR2) single nucleotide polymorphisms (SNPs) and: risk of PD or PD + T2D; periodontitis parameters in PD and PD + T2D; serum levels of cytokines/their receptors. Relationship between periodontal inflammation and serum cytokine/receptor levels was also assessed. Design: Subjects were stratified as: 57 healthy controls (HC); 58 PD; 65 PD + T2D. Sociodemographic, environmental, behavioral and periodontal clinical data were recorded. SNPs were genotyped using polymerase chain reaction-restriction fragment length polymorphism, while cytokines/receptors levels were quantified by enzyme-linked immunosorbent assay. Impact of periodontal inflammation was measured using periodontal inflamed surface area (PISA). Results: TNFα AA genotype showed protective effect in T2D + PD compared to PD, even adjusted for behavioral/environmental factors (OR 0.18; 95 %CI 0.037−0.886; p = 0.035). LTα AG heterozygotes had increased risk of PD (OR 3.27; 95 %CI 1.35−7.96; p = 0.016), while TNFR2 TG genotype had protective effect (OR = 0.44; 95 %CI 0.954−0.9794; p = 0.043). TNFR1 AA was predictor of periodontal pocket depth and clinical attachment loss in PD. Correlation between TNFR2 concentration and PISA was negative in PD, positive in PD + T2D. Conclusions: None of the SNPs showed cross-susceptibility between PD and T2D. + 252A/G LTα and +676 T/G TNFR2 SNPs are associated with PD risk. Periodontal destruction in healthy individuals is influenced by TNFR1 genotype. Impact of periodontal on systemic inflammation is masked by T2D.",
publisher = "Elsevier Ltd",
journal = "Archives of Oral Biology",
title = "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population",
volume = "120",
doi = "10.1016/j.archoralbio.2020.104929",
pages = "104929"
}

Matić Petrović, S., Nikolić, N., Toljić, B., Arambašić Jovanović, J., Miličić, B., Miličić, T., Jotić, A., Vidaković, M., Milašin, J.,& Pucar, A.. (2020). The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology
Elsevier Ltd., 120, 104929.
https://doi.org/10.1016/j.archoralbio.2020.104929

Matić Petrović S, Nikolić N, Toljić B, Arambašić Jovanović J, Miličić B, Miličić T, Jotić A, Vidaković M, Milašin J, Pucar A. The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population. in Archives of Oral Biology. 2020;120:104929.
doi:10.1016/j.archoralbio.2020.104929 .

Matić Petrović, Sanja, Nikolić, Nađa, Toljić, Boško, Arambašić Jovanović, Jelena, Miličić, Biljana, Miličić, Tanja, Jotić, Aleksandra, Vidaković, Melita, Milašin, Jelena, Pucar, Ana, "The association of tumor necrosis factor alpha, lymphotoxin alpha, tumor necrosis factor receptor 1 and tumor necrosis factor receptor 2 gene polymorphisms and serum levels with periodontitis and type 2 diabetes in Serbian population" in Archives of Oral Biology, 120 (2020):104929,
https://doi.org/10.1016/j.archoralbio.2020.104929 . .

TY  - CONF
AU  - Rajić, Jovana
AU  - Grdović, Nevena
AU  - Petrović Matić, Sanja
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Tolić, Anja
AU  - Đorđević, Marija
AU  - Đorđević, Miloš
AU  - Poznanović, Goran
AU  - Pucar, Ana
AU  - Vidaković, Melita
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5800
AB  - Objectives: Procalcitonin (PCT) has recently emerged as an important biomarker for an early and accurate diagnosis of bacte­rial infection, hence suggested as biomarker of periodontal disease caused by oral pathogenic microorganisms. Altered DNA meth­ylation of PCT coding gene - calcitonin-related polypeptide a (CALCA) has been shown during systemic inflammation. The aim of this study was to evaluate the influence oflocal and/or systemic inflammation present during periodontitis and diabetes on DNA methylation status of CALCA and its potential use as epigenetic­based biomarker of these chronic inflammatory conditions. 
Methods: The study included 65 individuals divided in three groups: healthy control (n = 17), periodontitis (n = 27) and diabe­tes/periodontitis group (n = 21). Periodontitis was diagnosed us­ing International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999) while type 2 diabetes as­sessment was performed according to WHO criteria (2013). DNA methylation profile of CALCA promoter in buccal epithelial cells was analyzed by methylation specific polymerase chain reaction (MSP). 
Results: Decrease in DNA methylation of CALCA promoter was observed in periodontitis and even more pronounced in dia­betes/periodontitis compared to control group, although without statistical significance. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CALCA promoter and glycosylated hemoglobin. Even though it is known that life style affects DNA methylation patterns, there was no difference in DNA methylation of CALCA promoter be­tween smokers/non-smokers and normal/overweight individuals. 
Conclusions: Presented results suggest that local periodontal inflammation contributes to the change, but that only systemic inflammation significantly alters the DNA methylation profile of CALCA in buccal cells. Those results imply that DNA methylation status of CALCA reflects systemic inflammation, but additional studies are needed to estimate usefulness of this epigenetic-based biomarker for periodontal disease.
PB  - Krager Publishers
C3  - Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy
T1  - Evaluation of the DNA Methylation Status of Procalcitonin Gene as a Biomarker of Local and Systemic Inflammation
DO  - 10.1159/000490753
SP  - 12
ER  - 

@conference{
author = "Rajić, Jovana and Grdović, Nevena and Petrović Matić, Sanja and Dinić, Svetlana and Uskoković, Aleksandra and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Tolić, Anja and Đorđević, Marija and Đorđević, Miloš and Poznanović, Goran and Pucar, Ana and Vidaković, Melita",
year = "2018",
abstract = "Objectives: Procalcitonin (PCT) has recently emerged as an important biomarker for an early and accurate diagnosis of bacte­rial infection, hence suggested as biomarker of periodontal disease caused by oral pathogenic microorganisms. Altered DNA meth­ylation of PCT coding gene - calcitonin-related polypeptide a (CALCA) has been shown during systemic inflammation. The aim of this study was to evaluate the influence oflocal and/or systemic inflammation present during periodontitis and diabetes on DNA methylation status of CALCA and its potential use as epigenetic­based biomarker of these chronic inflammatory conditions. 
Methods: The study included 65 individuals divided in three groups: healthy control (n = 17), periodontitis (n = 27) and diabe­tes/periodontitis group (n = 21). Periodontitis was diagnosed us­ing International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999) while type 2 diabetes as­sessment was performed according to WHO criteria (2013). DNA methylation profile of CALCA promoter in buccal epithelial cells was analyzed by methylation specific polymerase chain reaction (MSP). 
Results: Decrease in DNA methylation of CALCA promoter was observed in periodontitis and even more pronounced in dia­betes/periodontitis compared to control group, although without statistical significance. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CALCA promoter and glycosylated hemoglobin. Even though it is known that life style affects DNA methylation patterns, there was no difference in DNA methylation of CALCA promoter be­tween smokers/non-smokers and normal/overweight individuals. 
Conclusions: Presented results suggest that local periodontal inflammation contributes to the change, but that only systemic inflammation significantly alters the DNA methylation profile of CALCA in buccal cells. Those results imply that DNA methylation status of CALCA reflects systemic inflammation, but additional studies are needed to estimate usefulness of this epigenetic-based biomarker for periodontal disease.",
publisher = "Krager Publishers",
journal = "Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy",
title = "Evaluation of the DNA Methylation Status of Procalcitonin Gene as a Biomarker of Local and Systemic Inflammation",
doi = "10.1159/000490753",
pages = "12"
}

Rajić, J., Grdović, N., Petrović Matić, S., Dinić, S., Uskoković, A., Mihailović, M., Arambašić Jovanović, J., Tolić, A., Đorđević, M., Đorđević, M., Poznanović, G., Pucar, A.,& Vidaković, M.. (2018). Evaluation of the DNA Methylation Status of Procalcitonin Gene as a Biomarker of Local and Systemic Inflammation. in Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy
Krager Publishers., 12.
https://doi.org/10.1159/000490753

Rajić J, Grdović N, Petrović Matić S, Dinić S, Uskoković A, Mihailović M, Arambašić Jovanović J, Tolić A, Đorđević M, Đorđević M, Poznanović G, Pucar A, Vidaković M. Evaluation of the DNA Methylation Status of Procalcitonin Gene as a Biomarker of Local and Systemic Inflammation. in Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy. 2018;:12.
doi:10.1159/000490753 .

Rajić, Jovana, Grdović, Nevena, Petrović Matić, Sanja, Dinić, Svetlana, Uskoković, Aleksandra, Mihailović, Mirjana, Arambašić Jovanović, Jelena, Tolić, Anja, Đorđević, Marija, Đorđević, Miloš, Poznanović, Goran, Pucar, Ana, Vidaković, Melita, "Evaluation of the DNA Methylation Status of Procalcitonin Gene as a Biomarker of Local and Systemic Inflammation" in Selected Abstracts from the 3rd European Summer School on Nutrigenomics; 2018 Jun 25-29; Jesi, italy (2018):12,
https://doi.org/10.1159/000490753 . .

TY  - JOUR
AU  - Matić Petrović, Sanja
AU  - Pucar, Ana
AU  - Jotić, Aleksandra
AU  - Miličić, Biljana
AU  - Arambašić Jovanović, Jelena
AU  - Vidaković, Melita
AU  - Leković, Vojislav
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6315
AB  - Introduction: The role of tumor necrosis factor-α (TNFα) is well documented in pathogenesis of chronic periodontitis (CP) and type 2 diabetes (T2D). Considering short half-life of TNFα, tumor necrosis factor receptor-2 (TNFR2) is used as prosperous surrogate marker of TNFα activity.
Objective The aim was to detect TNFR2 serum concentration and correlate it with periodontal destruction in patients with diagnosed T2D and nondiabetics.
Methods The study included 85 patients divided into three groups: T2D + CP (group T2D, n = 34); nondiabetics + CP (Group PD, n = 27); and healthy controls (group HC, n = 24). T2D was diagnosed according to WHO criteria (2013) and periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999). TNFR2 level was measured by enzyme-linked immunosorbent assay (ELISA).
Results There was no difference in TNFR2 level among the groups (Kruskal-Wallis, p = 0.482). Significant correlation (Pearson's correlation coefficient) was observed between clinical attachment loss (CAL) and TNFR2 concentration in PD group (rp = -0.460, p = 0.016). In T2D group, correlations were observed between TNFR2 concentration and CaL (rp = 0.363, p = 0.005) and periodontal inflamed surface area (PISA) (rp = 0.345, p = 0.046) and periodontalepithelial surface area (PESA) (rp = 0.578, p = 0.000).
Conclusion Higher concentration of TNFR2 was associated with higher CAL, PESA, and PISA scores in T2D group. Contrary to that, nondiabetics with higher values of CAL exhibited lower concentration of TNFR2, presenting potential protective effect on periodontal destruction. These results imply that diabetes may alter TNFR2 secretion originated from periodontium.
PB  - Belgrade: Serbian Medical Society
T2  - Srpski arhiv za celokupno lekarstvo
T1  - Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study
IS  - 5-6
VL  - 144
DO  - 10.2298/SARH1606266M
SP  - 266
EP  - 272
ER  - 

@article{
author = "Matić Petrović, Sanja and Pucar, Ana and Jotić, Aleksandra and Miličić, Biljana and Arambašić Jovanović, Jelena and Vidaković, Melita and Leković, Vojislav",
year = "2016",
abstract = "Introduction: The role of tumor necrosis factor-α (TNFα) is well documented in pathogenesis of chronic periodontitis (CP) and type 2 diabetes (T2D). Considering short half-life of TNFα, tumor necrosis factor receptor-2 (TNFR2) is used as prosperous surrogate marker of TNFα activity.
Objective The aim was to detect TNFR2 serum concentration and correlate it with periodontal destruction in patients with diagnosed T2D and nondiabetics.
Methods The study included 85 patients divided into three groups: T2D + CP (group T2D, n = 34); nondiabetics + CP (Group PD, n = 27); and healthy controls (group HC, n = 24). T2D was diagnosed according to WHO criteria (2013) and periodontitis was diagnosed using International Workshop for a Classification of Periodontal Diseases and Conditions criteria (1999). TNFR2 level was measured by enzyme-linked immunosorbent assay (ELISA).
Results There was no difference in TNFR2 level among the groups (Kruskal-Wallis, p = 0.482). Significant correlation (Pearson's correlation coefficient) was observed between clinical attachment loss (CAL) and TNFR2 concentration in PD group (rp = -0.460, p = 0.016). In T2D group, correlations were observed between TNFR2 concentration and CaL (rp = 0.363, p = 0.005) and periodontal inflamed surface area (PISA) (rp = 0.345, p = 0.046) and periodontalepithelial surface area (PESA) (rp = 0.578, p = 0.000).
Conclusion Higher concentration of TNFR2 was associated with higher CAL, PESA, and PISA scores in T2D group. Contrary to that, nondiabetics with higher values of CAL exhibited lower concentration of TNFR2, presenting potential protective effect on periodontal destruction. These results imply that diabetes may alter TNFR2 secretion originated from periodontium.",
publisher = "Belgrade: Serbian Medical Society",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study",
number = "5-6",
volume = "144",
doi = "10.2298/SARH1606266M",
pages = "266-272"
}

Matić Petrović, S., Pucar, A., Jotić, A., Miličić, B., Arambašić Jovanović, J., Vidaković, M.,& Leković, V.. (2016). Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study. in Srpski arhiv za celokupno lekarstvo
Belgrade: Serbian Medical Society., 144(5-6), 266-272.
https://doi.org/10.2298/SARH1606266M

Matić Petrović S, Pucar A, Jotić A, Miličić B, Arambašić Jovanović J, Vidaković M, Leković V. Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study. in Srpski arhiv za celokupno lekarstvo. 2016;144(5-6):266-272.
doi:10.2298/SARH1606266M .

Matić Petrović, Sanja, Pucar, Ana, Jotić, Aleksandra, Miličić, Biljana, Arambašić Jovanović, Jelena, Vidaković, Melita, Leković, Vojislav, "Relationship between serum tumor necrosis factor receptor-2 concentration and periodontal destruction in patients with type 2 diabetes: Cross-sectional study" in Srpski arhiv za celokupno lekarstvo, 144, no. 5-6 (2016):266-272,
https://doi.org/10.2298/SARH1606266M . .

TY  - JOUR
AU  - Grdović, Nevena
AU  - Rajić, Jovana
AU  - Matić Petrović, Sanja
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Tolić, Anja
AU  - Pucar, Ana
AU  - Milašin, Jelena
AU  - Vidaković, Melita
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0003996916302229
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-84983475101&origin=resultslist&sort=plf-f&src=s&st1=Association+of+CXCL12+gene+promoter+methylation+with+periodontitis+in+patients+with+diabetes+mellitus&st2=&sid=55EA0C8D3BB1AC9032AD3C39F3D96943.wsnAw8kcdt7IPYLO0V48gA%3A420&sot=b&sdt=b&sl=116&s=TITLE-ABS-KEY%28Association+of+CXCL12+gene+promoter+methylation+with+periodontitis+in+patients+with+diabetes+mellitus%29&relpos=0&citeCnt=0&searchTerm=
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2543
AB  - Objectives CXCL12 is widely expressed, constitutive chemokine involved in tissue repair and regeneration, while the extent of its expression is important in various chronic inflammatory conditions. Involvement of DNA methylation in CXCL12 gene suppression (CXCL12) has been shown in malignancy and some autoimmune diseases. The aim of this study was to investigate whether the alterations in DNA methylation of CXCL12 are also involved in progression of periodontitis in combination with diabetes, as these chronic inflammatory conditions are strongly interrelated. Design Study included 72 subjects divided in three groups: healthy control (C, n = 21), periodontitis (P, n = 29) and diabetes/periodontitis group (D/P, n = 22). DNA extracted from epithelial cells obtained by sterile cotton swabs from buccal mucosa was subjected to methylation specific polymerase chain reaction (MSP) to obtain DNA methylation pattern of CXCL12 promoter. Results CXCL12 promoter was predominantly unmethylated in all groups. However, increase in the frequency of the methylated form and increase in percent of methylation of CXCL12 promoter in periodontitis and diabetes/periodontitis group compared to control group were found, although without statistical significance. However, statistically significant increase in Tm of MSP products in diabetes/periodontitis group was observed. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CXCL12 promoter and periodontal parameters, as well as between DNA methylation of CXCL12 and glycosylated hemoglobin. Conclusion Presented results suggest that chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease.
T2  - Archives of Oral Biology
T1  - Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2
VL  - 72
DO  - 10.1016/j.archoralbio.2016.08.025
SP  - 124
EP  - 133
ER  - 

@article{
author = "Grdović, Nevena and Rajić, Jovana and Matić Petrović, Sanja and Dinić, Svetlana and Uskoković, Aleksandra and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Tolić, Anja and Pucar, Ana and Milašin, Jelena and Vidaković, Melita",
year = "2016",
abstract = "Objectives CXCL12 is widely expressed, constitutive chemokine involved in tissue repair and regeneration, while the extent of its expression is important in various chronic inflammatory conditions. Involvement of DNA methylation in CXCL12 gene suppression (CXCL12) has been shown in malignancy and some autoimmune diseases. The aim of this study was to investigate whether the alterations in DNA methylation of CXCL12 are also involved in progression of periodontitis in combination with diabetes, as these chronic inflammatory conditions are strongly interrelated. Design Study included 72 subjects divided in three groups: healthy control (C, n = 21), periodontitis (P, n = 29) and diabetes/periodontitis group (D/P, n = 22). DNA extracted from epithelial cells obtained by sterile cotton swabs from buccal mucosa was subjected to methylation specific polymerase chain reaction (MSP) to obtain DNA methylation pattern of CXCL12 promoter. Results CXCL12 promoter was predominantly unmethylated in all groups. However, increase in the frequency of the methylated form and increase in percent of methylation of CXCL12 promoter in periodontitis and diabetes/periodontitis group compared to control group were found, although without statistical significance. However, statistically significant increase in Tm of MSP products in diabetes/periodontitis group was observed. Correlation analysis revealed statistically significant relationship between the extent of DNA methylation of the CXCL12 promoter and periodontal parameters, as well as between DNA methylation of CXCL12 and glycosylated hemoglobin. Conclusion Presented results suggest that chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease.",
journal = "Archives of Oral Biology",
title = "Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2",
volume = "72",
doi = "10.1016/j.archoralbio.2016.08.025",
pages = "124-133"
}

Grdović, N., Rajić, J., Matić Petrović, S., Dinić, S., Uskoković, A., Mihailović, M., Arambašić Jovanović, J., Tolić, A., Pucar, A., Milašin, J.,& Vidaković, M.. (2016). Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2. in Archives of Oral Biology, 72, 124-133.
https://doi.org/10.1016/j.archoralbio.2016.08.025

Grdović N, Rajić J, Matić Petrović S, Dinić S, Uskoković A, Mihailović M, Arambašić Jovanović J, Tolić A, Pucar A, Milašin J, Vidaković M. Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2. in Archives of Oral Biology. 2016;72:124-133.
doi:10.1016/j.archoralbio.2016.08.025 .

Grdović, Nevena, Rajić, Jovana, Matić Petrović, Sanja, Dinić, Svetlana, Uskoković, Aleksandra, Mihailović, Mirjana, Arambašić Jovanović, Jelena, Tolić, Anja, Pucar, Ana, Milašin, Jelena, Vidaković, Melita, "Association of CXCL12 gene promoter methylation with periodontitis in patients with diabetes mellitus type 2" in Archives of Oral Biology, 72 (2016):124-133,
https://doi.org/10.1016/j.archoralbio.2016.08.025 . .