TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đorđević, Ana
AU  - Romić, Snježana
AU  - Pantelić, Marija
AU  - Korićanac, Goran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5023
AB  - A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is
known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance,
though it is evident that dry cow muscles may contain increased triglyceride content. The current study
was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in
dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2
equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples
collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for
concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ),
glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine
palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus
muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows.
Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin
1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin
resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of
signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to
this disposition.
PB  - Amsterdam: Elsevier Ltd.
T2  - Research in Veterinary Science
T1  - Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle
VL  - 150
DO  - 10.1016/j.rvsc.2022.04.012
SP  - 189
EP  - 194
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đorđević, Ana and Romić, Snježana and Pantelić, Marija and Korićanac, Goran",
year = "2022",
abstract = "A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is
known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance,
though it is evident that dry cow muscles may contain increased triglyceride content. The current study
was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in
dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2
equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples
collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for
concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ),
glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine
palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus
muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows.
Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin
1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin
resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of
signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to
this disposition.",
publisher = "Amsterdam: Elsevier Ltd.",
journal = "Research in Veterinary Science",
title = "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle",
volume = "150",
doi = "10.1016/j.rvsc.2022.04.012",
pages = "189-194"
}

Prodanović, R., Kirovski, D., Vujanac, I., Đorđević, A., Romić, S., Pantelić, M.,& Korićanac, G.. (2022). Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science
Amsterdam: Elsevier Ltd.., 150, 189-194.
https://doi.org/10.1016/j.rvsc.2022.04.012

Prodanović R, Kirovski D, Vujanac I, Đorđević A, Romić S, Pantelić M, Korićanac G. Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science. 2022;150:189-194.
doi:10.1016/j.rvsc.2022.04.012 .

Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đorđević, Ana, Romić, Snježana, Pantelić, Marija, Korićanac, Goran, "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle" in Research in Veterinary Science, 150 (2022):189-194,
https://doi.org/10.1016/j.rvsc.2022.04.012 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Vujanac, Ivan
AU  - Đorđević, Ana
AU  - Pantelić, Marija
AU  - Romić, Snježana
AU  - Stanimirović, Zoran
AU  - Kirovski, Danijela
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6086
AB  - We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepaticfatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groupsbased on their body condition score (BCS) as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Intravenousglucose tolerance test (GTT) and liver biopsieswere carried out at day 10 before calving. Blood samples were collectedbefore (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levelswere determined at each sample point. In addition, β-hydroxybutyrate and triglycerides levels were measuredin the basal samples. The liver biopsieswere analyzed for total lipid content and protein expression of insulin receptorbeta (IRβ), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1).Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglyceridesand hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The developmentof insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression ofthe IRβ, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevantin the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
PB  - Elsevier
T2  - Research in Veterinary Science
T1  - Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
VL  - 107
DO  - 10.1016/j.rvsc.2016.04.007
SP  - 16
EP  - 19
ER  - 

@article{
author = "Prodanović, Radiša and Korićanac, Goran and Vujanac, Ivan and Đorđević, Ana and Pantelić, Marija and Romić, Snježana and Stanimirović, Zoran and Kirovski, Danijela",
year = "2016",
abstract = "We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepaticfatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groupsbased on their body condition score (BCS) as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Intravenousglucose tolerance test (GTT) and liver biopsieswere carried out at day 10 before calving. Blood samples were collectedbefore (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levelswere determined at each sample point. In addition, β-hydroxybutyrate and triglycerides levels were measuredin the basal samples. The liver biopsieswere analyzed for total lipid content and protein expression of insulin receptorbeta (IRβ), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1).Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglyceridesand hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The developmentof insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression ofthe IRβ, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevantin the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.",
publisher = "Elsevier",
journal = "Research in Veterinary Science",
title = "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression",
volume = "107",
doi = "10.1016/j.rvsc.2016.04.007",
pages = "16-19"
}

Prodanović, R., Korićanac, G., Vujanac, I., Đorđević, A., Pantelić, M., Romić, S., Stanimirović, Z.,& Kirovski, D.. (2016). Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science
Elsevier., 107, 16-19.
https://doi.org/10.1016/j.rvsc.2016.04.007

Prodanović R, Korićanac G, Vujanac I, Đorđević A, Pantelić M, Romić S, Stanimirović Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science. 2016;107:16-19.
doi:10.1016/j.rvsc.2016.04.007 .

Prodanović, Radiša, Korićanac, Goran, Vujanac, Ivan, Đorđević, Ana, Pantelić, Marija, Romić, Snježana, Stanimirović, Zoran, Kirovski, Danijela, "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression" in Research in Veterinary Science, 107 (2016):16-19,
https://doi.org/10.1016/j.rvsc.2016.04.007 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Korićanac, Goran
AU  - Vujanac, Ivan
AU  - Đorđević, Ana
AU  - Pantelić, Marija
AU  - Romić, Snježana
AU  - Stanimirović, Zoran
AU  - Kirovski, Danijela
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6085
AB  - We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic
fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups
based on their body condition score (BCS) as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Intravenous
glucose tolerance test (GTT) and liver biopsieswere carried out at day 10 before calving. Blood samples were collected
before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels
were determined at each sample point. In addition, β-hydroxybutyrate and triglycerides levels were measured
in the basal samples. The liver biopsieswere analyzed for total lipid content and protein expression of insulin receptor
beta (IRβ), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1).
Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides
and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development
of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of
the IRβ, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant
in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.
PB  - Elsevier
T2  - Research in Veterinary Science
T1  - Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression
VL  - 107
DO  - 10.1016/j.rvsc.2016.04.007
SP  - 16
EP  - 19
ER  - 

@article{
author = "Prodanović, Radiša and Korićanac, Goran and Vujanac, Ivan and Đorđević, Ana and Pantelić, Marija and Romić, Snježana and Stanimirović, Zoran and Kirovski, Danijela",
year = "2016",
abstract = "We investigated the hypothesis that obesity in dairy cows enhanced expression of proteins involved in hepatic
fatty acid uptake and metabolism. Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups
based on their body condition score (BCS) as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Intravenous
glucose tolerance test (GTT) and liver biopsieswere carried out at day 10 before calving. Blood samples were collected
before (basal) and after glucose infusion, and glucose, insulin and non-esterified fatty acid (NEFA) levels
were determined at each sample point. In addition, β-hydroxybutyrate and triglycerides levels were measured
in the basal samples. The liver biopsieswere analyzed for total lipid content and protein expression of insulin receptor
beta (IRβ), fatty acid translocase (FAT/CD36) and sterol regulatory element-binding protein-1 (SREBP-1).
Basal glucose and insulin were higher in high-BCS cows, which coincided with higher circulating triglycerides
and hepatic lipid content. Clearance rate and AUC for NEFA during GTT were higher in optimal-BCS cows. The development
of insulin resistance and fatty liver in obese cows was paralleled by increased hepatic expression of
the IRβ, CD36 and SREBP-1. These results suggest that increased expression of hepatic CD36 and SREBP-1 is relevant
in the obesity-driven lipid accumulation in the liver of dairy cows during late gestation.",
publisher = "Elsevier",
journal = "Research in Veterinary Science",
title = "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression",
volume = "107",
doi = "10.1016/j.rvsc.2016.04.007",
pages = "16-19"
}

Prodanović, R., Korićanac, G., Vujanac, I., Đorđević, A., Pantelić, M., Romić, S., Stanimirović, Z.,& Kirovski, D.. (2016). Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science
Elsevier., 107, 16-19.
https://doi.org/10.1016/j.rvsc.2016.04.007

Prodanović R, Korićanac G, Vujanac I, Đorđević A, Pantelić M, Romić S, Stanimirović Z, Kirovski D. Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression. in Research in Veterinary Science. 2016;107:16-19.
doi:10.1016/j.rvsc.2016.04.007 .

Prodanović, Radiša, Korićanac, Goran, Vujanac, Ivan, Đorđević, Ana, Pantelić, Marija, Romić, Snježana, Stanimirović, Zoran, Kirovski, Danijela, "Obesity-driven prepartal hepatic lipid accumulation in dairy cows is associated with increased CD36 and SREBP-1 expression" in Research in Veterinary Science, 107 (2016):16-19,
https://doi.org/10.1016/j.rvsc.2016.04.007 . .