TY  - JOUR
AU  - Prijović, Mladen
AU  - Nikolić, Bogdan
AU  - Dragićević, Ivana
AU  - Nestorović Živković, Jasmina
AU  - Dmitrović, Slavica
AU  - Giba, Zlatko
AU  - Jovanović, Vladan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6562
AB  - Plant protection with natural products is a new trend in environmentally friendly agriculture.
Nepeta rtanjensis Diklić et Milojević is an endemic and critically endangered plant species in Serbia.
We explored the phytotoxic potential of a water emulsion of Nepeta rtanjensis essential oil (NrEO)
with high amounts of trans,cis-nepetalactone on five weeds. The most sensitive was Stellaria media
(L.) Vill., as NrEO (from 0.013% to 0.1%) completely inhibited germination. Germination of
Amaranthus retroflexus L. and Artemisia vulgaris L. was completely inhibited at the highest applied
concentrations of NrEO (0.1%), while Ambrosia artemisiifolia L. germination was reduced to 48% at
the same concentration of NrEO. The most tolerant species was Cephalaria transsylvanica (L.) Schrad.
ex Roem. & Schult. as the final germination rate at the highest applied concentration of NrEO (0.1%)
was 81%, like the control (82%). To our knowledge, this is the first time the interaction of essential oils
on the germination and growth of A. vulgaris and C. transsylvanica is reported. The germination
dynamics of S. media in pots with soil were significantly inhibited when the soil was initially treated
with the highest applied concentration of the NrEO water emulsion (1%). Spraying S. media seedlings
with NrEO significantly inhibited growth parameters (shoot height, shoot fresh weight, and the number
of nodes) at the higher applied concentrations of NrEO (0.5% and 1%). Therefore, the water emulsion
of the essential oil of N. rtanjensis could be potentially developed for use in the control of invasive and
allergenic weeds.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide
DO  - 10.2298/ABS231107041P
ER  - 

@article{
author = "Prijović, Mladen and Nikolić, Bogdan and Dragićević, Ivana and Nestorović Živković, Jasmina and Dmitrović, Slavica and Giba, Zlatko and Jovanović, Vladan",
year = "2023",
abstract = "Plant protection with natural products is a new trend in environmentally friendly agriculture.
Nepeta rtanjensis Diklić et Milojević is an endemic and critically endangered plant species in Serbia.
We explored the phytotoxic potential of a water emulsion of Nepeta rtanjensis essential oil (NrEO)
with high amounts of trans,cis-nepetalactone on five weeds. The most sensitive was Stellaria media
(L.) Vill., as NrEO (from 0.013% to 0.1%) completely inhibited germination. Germination of
Amaranthus retroflexus L. and Artemisia vulgaris L. was completely inhibited at the highest applied
concentrations of NrEO (0.1%), while Ambrosia artemisiifolia L. germination was reduced to 48% at
the same concentration of NrEO. The most tolerant species was Cephalaria transsylvanica (L.) Schrad.
ex Roem. & Schult. as the final germination rate at the highest applied concentration of NrEO (0.1%)
was 81%, like the control (82%). To our knowledge, this is the first time the interaction of essential oils
on the germination and growth of A. vulgaris and C. transsylvanica is reported. The germination
dynamics of S. media in pots with soil were significantly inhibited when the soil was initially treated
with the highest applied concentration of the NrEO water emulsion (1%). Spraying S. media seedlings
with NrEO significantly inhibited growth parameters (shoot height, shoot fresh weight, and the number
of nodes) at the higher applied concentrations of NrEO (0.5% and 1%). Therefore, the water emulsion
of the essential oil of N. rtanjensis could be potentially developed for use in the control of invasive and
allergenic weeds.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide",
doi = "10.2298/ABS231107041P"
}

Prijović, M., Nikolić, B., Dragićević, I., Nestorović Živković, J., Dmitrović, S., Giba, Z.,& Jovanović, V.. (2023). Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide. in Archives of Biological Sciences
Belgrade: Serbian Biological Society..
https://doi.org/10.2298/ABS231107041P

Prijović M, Nikolić B, Dragićević I, Nestorović Živković J, Dmitrović S, Giba Z, Jovanović V. Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide. in Archives of Biological Sciences. 2023;.
doi:10.2298/ABS231107041P .

Prijović, Mladen, Nikolić, Bogdan, Dragićević, Ivana, Nestorović Živković, Jasmina, Dmitrović, Slavica, Giba, Zlatko, Jovanović, Vladan, "Water emulsion of the essential oil of Nepeta rtanjensis Diklić & Milojević: Potential use as a bioherbicide" in Archives of Biological Sciences (2023),
https://doi.org/10.2298/ABS231107041P . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Paunović, Danijela
AU  - Milošević, Snežana
AU  - Marković, Marija
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana
AU  - Subotić, Angelina
PY  - 2020
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/aab.12593
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3690
AB  - Common centaury (Centaurium erythraea Rafn.) is a plant species that can inhabit saline soils. It is known as a plant with high spontaneous regeneration potential in vitro. In the present work we evaluated shoots and roots salinity tolerance of non-transformed and three AtCKX transgenic centaury lines to graded NaCl concentrations (0, 50, 100, 150, 200 mM) in vitro. Overexpression of AtCKX genes in transgenic centaury plants resulted in an altered cytokinins (CKs) profile leading to a decline of bioactive CK levels and, at the same time, increased contents of storage CK forms, inactive CK forms and/or CK nucleotides. Significant increment of fresh shoot weight was obtained in shoots of non-transformed and AtCKX1 transgenic line only on medium supplemented with 50 mM NaCl. However two analysed AtCKX2 transgenic lines reduced shoot growth at all NaCl concentrations. In general, centaury roots showed higher tolerance to salinity than shoots. Non-transformed and AtCKX1 transgenic lines tolerated up to 100 mM NaCl without change in frequency of regeneration and number of regenerated plants. Roots of two analysed AtCKX2 transgenic lines showed different regeneration potential under salt stress. Regeneration of transgenic AtCKX2-26 shoots even at 200 mM NaCl was recorded. Salinity stress response of centaury shoots and roots was also evaluated at biochemical level. Free proline, malondialdehyde and hydrogen peroxide content as well as antioxidative enzymes activities were investigated in shoots and roots after 1, 2, 4 and 8 weeks. In general, adition of NaCl in culture medium elevated all biochemical parameters in centaury shoots and in roots. Considering that all analysed AtCKX transgenic centaury lines showed altered salt tolerance to graded NaCl concentrations in vitro it can be assumed that CKs might be involved in plant defence to salt stress conditions.
PB  - Blackwell Publishing Ltd
T2  - Annals of Applied Biology
T1  - Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro
DO  - 10.1111/aab.12593
SP  - aab.12593
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Paunović, Danijela and Milošević, Snežana and Marković, Marija and Jevremović, Slađana and Dragićević, Ivana and Subotić, Angelina",
year = "2020",
abstract = "Common centaury (Centaurium erythraea Rafn.) is a plant species that can inhabit saline soils. It is known as a plant with high spontaneous regeneration potential in vitro. In the present work we evaluated shoots and roots salinity tolerance of non-transformed and three AtCKX transgenic centaury lines to graded NaCl concentrations (0, 50, 100, 150, 200 mM) in vitro. Overexpression of AtCKX genes in transgenic centaury plants resulted in an altered cytokinins (CKs) profile leading to a decline of bioactive CK levels and, at the same time, increased contents of storage CK forms, inactive CK forms and/or CK nucleotides. Significant increment of fresh shoot weight was obtained in shoots of non-transformed and AtCKX1 transgenic line only on medium supplemented with 50 mM NaCl. However two analysed AtCKX2 transgenic lines reduced shoot growth at all NaCl concentrations. In general, centaury roots showed higher tolerance to salinity than shoots. Non-transformed and AtCKX1 transgenic lines tolerated up to 100 mM NaCl without change in frequency of regeneration and number of regenerated plants. Roots of two analysed AtCKX2 transgenic lines showed different regeneration potential under salt stress. Regeneration of transgenic AtCKX2-26 shoots even at 200 mM NaCl was recorded. Salinity stress response of centaury shoots and roots was also evaluated at biochemical level. Free proline, malondialdehyde and hydrogen peroxide content as well as antioxidative enzymes activities were investigated in shoots and roots after 1, 2, 4 and 8 weeks. In general, adition of NaCl in culture medium elevated all biochemical parameters in centaury shoots and in roots. Considering that all analysed AtCKX transgenic centaury lines showed altered salt tolerance to graded NaCl concentrations in vitro it can be assumed that CKs might be involved in plant defence to salt stress conditions.",
publisher = "Blackwell Publishing Ltd",
journal = "Annals of Applied Biology",
title = "Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro",
doi = "10.1111/aab.12593",
pages = "aab.12593"
}

Trifunović-Momčilov, M., Paunović, D., Milošević, S., Marković, M., Jevremović, S., Dragićević, I.,& Subotić, A.. (2020). Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro. in Annals of Applied Biology
Blackwell Publishing Ltd., aab.12593.
https://doi.org/10.1111/aab.12593

Trifunović-Momčilov M, Paunović D, Milošević S, Marković M, Jevremović S, Dragićević I, Subotić A. Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro. in Annals of Applied Biology. 2020;:aab.12593.
doi:10.1111/aab.12593 .

Trifunović-Momčilov, Milana, Paunović, Danijela, Milošević, Snežana, Marković, Marija, Jevremović, Slađana, Dragićević, Ivana, Subotić, Angelina, "Salinity stress response of non‐transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn.) shoots and roots grown in vitro" in Annals of Applied Biology (2020):aab.12593,
https://doi.org/10.1111/aab.12593 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Dobrev, Petre I.
AU  - Malbeck, Jiří
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2020
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7044434
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3623
AB  - A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.
T2  - Scientific Reports
T1  - Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60412-9
SP  - 3437
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Dobrev, Petre I. and Malbeck, Jiří and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Tadić, Vojin and Dragićević, Ivana",
year = "2020",
abstract = "A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.",
journal = "Scientific Reports",
title = "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60412-9",
pages = "3437"
}

Raspor, M., Motyka, V., Ninković, S., Dobrev, P. I., Malbeck, J., Ćosić, T., Cingel, A., Savić, J., Tadić, V.,& Dragićević, I.. (2020). Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports, 10(1), 3437.
https://doi.org/10.1038/s41598-020-60412-9

Raspor M, Motyka V, Ninković S, Dobrev PI, Malbeck J, Ćosić T, Cingel A, Savić J, Tadić V, Dragićević I. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports. 2020;10(1):3437.
doi:10.1038/s41598-020-60412-9 .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Dobrev, Petre I., Malbeck, Jiří, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Tadić, Vojin, Dragićević, Ivana, "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics." in Scientific Reports, 10, no. 1 (2020):3437,
https://doi.org/10.1038/s41598-020-60412-9 . .

TY  - CONF
AU  - Dragićević, Ivana
AU  - Kovačević, Milica
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
AU  - Raspor, Martin
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4320
AB  - Transformed potato plants, bearing genes for cytokinin oxidase/dehydrogenase (CKX), the key enzyme in cytokinin (CK) catabolism, represent a valuable model-system for investigations of CK role in the fine tuning of tuberization. CKs are known to affect tuber initiation in vitro, while their effects on other processes, such as tuber enlargement and dormancy break, are less clear. In this study we have investigated dynamics of tuber formation (observed every 5 days during 30-day long experimental period) and parameters of tuber enlargement (tuber diameter and mass measured at the end of experiment) under strong tuber-inducing conditions in vitro (continuous darkness and high sucrose concentration [8% w/v] in the medium) in two transformed potato lines, bearing AtCKX1 or AtCKX2 genes. As previously shown, selected transformed potato lines grown in vitro were characterized by slightly elevated (line AtCKX1-36a) or manifoldly increased (line AtCKX2-51) CKX activity, resulted in differently reduced bioactive CK contents. 6-benzyladenine (BA), being neither substrate nor inhibitor of CKX, was added to the medium (at the concentration of 1 μM) in order to investigate whether it could substitute for the lack of endogenous CKs and their effect on tuberization in AtCKX potato lines. Tuber initiation on single node stem cuttings showed different dynamics in two investigated AtCKX lines, although in both lines it was completed earlier than in non-transformed control. BA at 1 μM enhanced and accelerated tuber formation in control and AtCKX1-36a. In both AtCKX lines, tuber diameter and mass were decreased, which could be restored to control values by addition of BA. BA also shortened tuber dormancy.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia
T1  - Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4320
ER  - 

@conference{
editor = "Uzelac, Branka",
author = "Dragićević, Ivana and Kovačević, Milica and Motyka, Vaclav and Ninković, Slavica and Raspor, Martin",
year = "2018",
abstract = "Transformed potato plants, bearing genes for cytokinin oxidase/dehydrogenase (CKX), the key enzyme in cytokinin (CK) catabolism, represent a valuable model-system for investigations of CK role in the fine tuning of tuberization. CKs are known to affect tuber initiation in vitro, while their effects on other processes, such as tuber enlargement and dormancy break, are less clear. In this study we have investigated dynamics of tuber formation (observed every 5 days during 30-day long experimental period) and parameters of tuber enlargement (tuber diameter and mass measured at the end of experiment) under strong tuber-inducing conditions in vitro (continuous darkness and high sucrose concentration [8% w/v] in the medium) in two transformed potato lines, bearing AtCKX1 or AtCKX2 genes. As previously shown, selected transformed potato lines grown in vitro were characterized by slightly elevated (line AtCKX1-36a) or manifoldly increased (line AtCKX2-51) CKX activity, resulted in differently reduced bioactive CK contents. 6-benzyladenine (BA), being neither substrate nor inhibitor of CKX, was added to the medium (at the concentration of 1 μM) in order to investigate whether it could substitute for the lack of endogenous CKs and their effect on tuberization in AtCKX potato lines. Tuber initiation on single node stem cuttings showed different dynamics in two investigated AtCKX lines, although in both lines it was completed earlier than in non-transformed control. BA at 1 μM enhanced and accelerated tuber formation in control and AtCKX1-36a. In both AtCKX lines, tuber diameter and mass were decreased, which could be restored to control values by addition of BA. BA also shortened tuber dormancy.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia",
title = "Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro",
pages = "26",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4320"
}

Uzelac, B., Dragićević, I., Kovačević, M., Motyka, V., Ninković, S.,& Raspor, M.. (2018). Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro. in 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 26.
https://hdl.handle.net/21.15107/rcub_ibiss_4320

Uzelac B, Dragićević I, Kovačević M, Motyka V, Ninković S, Raspor M. Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro. in 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia. 2018;:26.
https://hdl.handle.net/21.15107/rcub_ibiss_4320 .

Uzelac, Branka, Dragićević, Ivana, Kovačević, Milica, Motyka, Vaclav, Ninković, Slavica, Raspor, Martin, "Effects of 6-benzyladenine on the tuberization process in two AtCKX-transformed potato lines under strong tuber-inducing conditions in vitro" in 3rd International Conference on Plant Biology / 22nd SPPS Meeting, 9-12 June 2018, Belgrade, Serbia (2018):26,
https://hdl.handle.net/21.15107/rcub_ibiss_4320 .

TY  - CONF
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Vaclav
AU  - Dragićević, Ivana
AU  - Paunović, Danijela
AU  - Marković, Marija
AU  - Subotić, Angelina
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6322
AB  - Common centaury (Centaurium erythraea Rafn) is a medicinal plant that can inhabit saline soils.
Previous in vitro studies revealed centaury’s tolerance to salinity concerning shoot regeneration
and development. Centaury is known to spontaneously regenerate shoots from root segments
in vitro on hormone-free medium, probably due to endogenous levels of cytokinins (CKs) and
indolyl-3-acetic acid and their balance in roots. In this study we have investigated in vitro shoot
regeneration from root segments, i.e. frequency of shoot regeneration, the average number of regenerated shoots per explant and shoot biomass production in three transformed centaury lines,
bearing AtCKX1 or AtCKX2 genes. Graded concentrations of NaCl (0–200 mM) were added to the
nutrition medium during 8-week cultivation period. As previously shown, selected transformed
centaury lines grown in vitro were characterized by differently reduced bioactive CK contents, as a
consequence of different expression of transgenes. The main goal was to elucidate if changed CK
homeostasis interferes with salt-tolerance and affects shoot regeneration in centaury. The selected AtCKX centaury lines significantly differed from each other in terms of capacity for spontaneous
shoot regeneration and biomass production in vitro. NaCl lowered shoot regeneration in all of
the investigated lines, although they differed in their sensitivity to salt. Both AtCKX2 lines showed
increased sensitivity to NaCl with the highest sensitivity found in one with the highest potential for
spontaneous regeneration (AtCKX2-26). Concentration of 50 mM NaCl enhanced biomass production only in control and line AtCKX1-29. Our results indicate connection between CK homeostasis,
regeneration capacity and tolerance to salinity.
PB  - elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro
SP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6322
ER  - 

@conference{
author = "Trifunović-Momčilov, Milana and Motyka, Vaclav and Dragićević, Ivana and Paunović, Danijela and Marković, Marija and Subotić, Angelina",
year = "2018",
abstract = "Common centaury (Centaurium erythraea Rafn) is a medicinal plant that can inhabit saline soils.
Previous in vitro studies revealed centaury’s tolerance to salinity concerning shoot regeneration
and development. Centaury is known to spontaneously regenerate shoots from root segments
in vitro on hormone-free medium, probably due to endogenous levels of cytokinins (CKs) and
indolyl-3-acetic acid and their balance in roots. In this study we have investigated in vitro shoot
regeneration from root segments, i.e. frequency of shoot regeneration, the average number of regenerated shoots per explant and shoot biomass production in three transformed centaury lines,
bearing AtCKX1 or AtCKX2 genes. Graded concentrations of NaCl (0–200 mM) were added to the
nutrition medium during 8-week cultivation period. As previously shown, selected transformed
centaury lines grown in vitro were characterized by differently reduced bioactive CK contents, as a
consequence of different expression of transgenes. The main goal was to elucidate if changed CK
homeostasis interferes with salt-tolerance and affects shoot regeneration in centaury. The selected AtCKX centaury lines significantly differed from each other in terms of capacity for spontaneous
shoot regeneration and biomass production in vitro. NaCl lowered shoot regeneration in all of
the investigated lines, although they differed in their sensitivity to salt. Both AtCKX2 lines showed
increased sensitivity to NaCl with the highest sensitivity found in one with the highest potential for
spontaneous regeneration (AtCKX2-26). Concentration of 50 mM NaCl enhanced biomass production only in control and line AtCKX1-29. Our results indicate connection between CK homeostasis,
regeneration capacity and tolerance to salinity.",
publisher = "elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro",
pages = "33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6322"
}

Trifunović-Momčilov, M., Motyka, V., Dragićević, I., Paunović, D., Marković, M.,& Subotić, A.. (2018). Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
elgrade: Serbian Plant Physiology Society; Institute for Biological Research "Siniša Stanković"; Faculty of Biology., 33.
https://hdl.handle.net/21.15107/rcub_ibiss_6322

Trifunović-Momčilov M, Motyka V, Dragićević I, Paunović D, Marković M, Subotić A. Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:33.
https://hdl.handle.net/21.15107/rcub_ibiss_6322 .

Trifunović-Momčilov, Milana, Motyka, Vaclav, Dragićević, Ivana, Paunović, Danijela, Marković, Marija, Subotić, Angelina, "Shoot regeneration from root segments in AtCKX transformed centaury lines grown at graded NaCl concentrations in vitro" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):33,
https://hdl.handle.net/21.15107/rcub_ibiss_6322 .

TY  - CONF
AU  - Trailović, Maja
AU  - Dragićević, Ivana
AU  - Raspor, Martin
AU  - Motyka, Vaclav
AU  - Ninković, Slavica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4321
AB  - Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK). SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.
PB  - Belgrade: Serbian Biological Society
C3  - Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija
T1  - Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro
SP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4321
ER  - 

@conference{
author = "Trailović, Maja and Dragićević, Ivana and Raspor, Martin and Motyka, Vaclav and Ninković, Slavica",
year = "2018",
abstract = "Salicilna kiselina (SA) je biljni regulator rastenja čije su glavne fiziološke funkcije odbrana od biotičkog stresa i termogeneza tokom cvetanja kod određenih biljnih vrsta. U novije vreme sve je više podataka o ulozi SA u odbrani biljaka od abiotičkog stresa. SA se često označava kao biljni hormon, mada su podaci o njenim specifičnim efektima na rastenje i razviće kod biljaka sporadični i ograničeni na mali broj vrsta. U literaturi nema podataka o efektima SA na rastenje i razviće krompira, kao ni o interakciji SA sa biljnim hormonima koji regulišu ekonomski značajan proces tuberizacije kod ove vrste. Cilj sprovedenog istraživanja je bio da se utvrdi da li egzogeno primenjena SA ima efekte na morfološke osobine i tuberizaciju kod krompira gajenog in vitro. Ispitivan je efekat SA kod netransformisanog krompira (K) i jedne linije AtCKX2-transformisanog krompira (AtCKX2-51), koju karakteriše veoma nizak sadržaj bioaktivnih citokinina (CK). SA je primenjivana u niskim koncentracijama (0,01–10 μM), dodavanjem u podloge za gajenje izdanaka krompira in vitro, a morfološki parametri, uključujući i parametre tuberizacije, određivani su u kulturama starim 30 dana. Rezultati su pokazali da SA (u najvećoj primenjenoj koncentraciji) ima efekat samo na pojedine procese rastenja i razvića, a da ne remeti tuberizaciju. Uočeni efekti se razlikuju kod K i AtCKX2-51 krompira. Prikazani rezultati sprovedenog istraživanja ukazuju na moguću interakciju između SA i CK u regulaciji rastenja i razvića krompira in vitro.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija",
title = "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro",
pages = "62",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4321"
}

Trailović, M., Dragićević, I., Raspor, M., Motyka, V.,& Ninković, S.. (2018). Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro. in Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija
Belgrade: Serbian Biological Society., 62.
https://hdl.handle.net/21.15107/rcub_ibiss_4321

Trailović M, Dragićević I, Raspor M, Motyka V, Ninković S. Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro. in Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija. 2018;:62.
https://hdl.handle.net/21.15107/rcub_ibiss_4321 .

Trailović, Maja, Dragićević, Ivana, Raspor, Martin, Motyka, Vaclav, Ninković, Slavica, "Efekti salicilne kiseline na morfološke osobine i formiranje krtola kod netransformisanog i AtCKX2-transformisanog krompira (Solanum tuberosum L. cv. Désirée) gajenog in vitro" in Drugi kongres biologa Srbije, 25-30. septembra 2018, Kladovo, Srbija (2018):62,
https://hdl.handle.net/21.15107/rcub_ibiss_4321 .

TY  - JOUR
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Bogdanović, Milica
AU  - Subotić, Angelina
AU  - Ghalawenji, Nabil
AU  - Dragićević, Ivana
AU  - Todorović, Slađana
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2539
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/782
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641500087D
UR  - 000377883900017
AB  - Primary and secondary ammonium assimilation is catalyzed by the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway in plants. The Arabidopsis genome contains five cytosolic GS1 genes (GLN1;1 - GLN1;5), one nuclear gene for chloroplastic GS2 isoform (GLN2), two Fd-GOGAT genes (GLU1 and GLU2) and a GLT1 gene coding for NADH-GOGAT. Even though the regulation of GS and GOGAT isoforms has been extensively studied in response to various environmental and metabolic cues in many plant species, little is known about the effects of phytohormones on their regulation. The objective of this study was to investigate the impact of representative plant growth regulators, kinetin (KIN), abscisic acid (ABA), gibberellic acid (GA3) and 2,4-dichlorophenoxyacetic acid (2,4-D), on the expression of A. thaliana GS and GOGAT genes. The obtained results indicate that GS and GOGAT genes are differentially regulated by growth regulators in shoots and roots. KIN and 2,4-D repressed GS and GOGAT expression in roots, with little effect on transcript levels in shoots. KIN affected all tested genes; 2,4-D was apparently more selective and less potent. ABA induced the expression of GLN1;1 and GLU2 in whole seedlings, while GA3 enhanced the expression of all tested genes in shoots, except GLU2. The observed expression patterns are discussed in relation to physiological roles of investigated plant growth regulators and N-assimilating enzymes.
T2  - Archives of Biological Sciences
T1  - Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings
IS  - 2
VL  - 68
DO  - 10.2298/ABS150608087D
SP  - 399
EP  - 404
ER  - 

@article{
author = "Dragićević, Milan and Simonović, Ana and Bogdanović, Milica and Subotić, Angelina and Ghalawenji, Nabil and Dragićević, Ivana and Todorović, Slađana",
year = "2016",
abstract = "Primary and secondary ammonium assimilation is catalyzed by the glutamine synthetase-glutamate synthase (GS-GOGAT) pathway in plants. The Arabidopsis genome contains five cytosolic GS1 genes (GLN1;1 - GLN1;5), one nuclear gene for chloroplastic GS2 isoform (GLN2), two Fd-GOGAT genes (GLU1 and GLU2) and a GLT1 gene coding for NADH-GOGAT. Even though the regulation of GS and GOGAT isoforms has been extensively studied in response to various environmental and metabolic cues in many plant species, little is known about the effects of phytohormones on their regulation. The objective of this study was to investigate the impact of representative plant growth regulators, kinetin (KIN), abscisic acid (ABA), gibberellic acid (GA3) and 2,4-dichlorophenoxyacetic acid (2,4-D), on the expression of A. thaliana GS and GOGAT genes. The obtained results indicate that GS and GOGAT genes are differentially regulated by growth regulators in shoots and roots. KIN and 2,4-D repressed GS and GOGAT expression in roots, with little effect on transcript levels in shoots. KIN affected all tested genes; 2,4-D was apparently more selective and less potent. ABA induced the expression of GLN1;1 and GLU2 in whole seedlings, while GA3 enhanced the expression of all tested genes in shoots, except GLU2. The observed expression patterns are discussed in relation to physiological roles of investigated plant growth regulators and N-assimilating enzymes.",
journal = "Archives of Biological Sciences",
title = "Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings",
number = "2",
volume = "68",
doi = "10.2298/ABS150608087D",
pages = "399-404"
}

Dragićević, M., Simonović, A., Bogdanović, M., Subotić, A., Ghalawenji, N., Dragićević, I.,& Todorović, S.. (2016). Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings. in Archives of Biological Sciences, 68(2), 399-404.
https://doi.org/10.2298/ABS150608087D

Dragićević M, Simonović A, Bogdanović M, Subotić A, Ghalawenji N, Dragićević I, Todorović S. Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings. in Archives of Biological Sciences. 2016;68(2):399-404.
doi:10.2298/ABS150608087D .

Dragićević, Milan, Simonović, Ana, Bogdanović, Milica, Subotić, Angelina, Ghalawenji, Nabil, Dragićević, Ivana, Todorović, Slađana, "Differential regulation of GS-GOGAT gene expression by plant growth regulators in Arabidopsis seedlings" in Archives of Biological Sciences, 68, no. 2 (2016):399-404,
https://doi.org/10.2298/ABS150608087D . .

TY  - JOUR
AU  - Savić, Jelena
AU  - Dragićević, Ivana
AU  - Pantelić, D.
AU  - Oljača, Jasmina
AU  - Momčilović, Ivana
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/298
AB  - We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.
T2  - Archives of Biological Sciences
T1  - Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)
IS  - 1
VL  - 64
SP  - 135
EP  - 144
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_298
ER  - 

@article{
author = "Savić, Jelena and Dragićević, Ivana and Pantelić, D. and Oljača, Jasmina and Momčilović, Ivana",
year = "2012, 2012",
abstract = "We have examined the correlation between heat tolerance and small heat shock protein (sHSP) expression under heat stress conditions in potato (Solanum tuberosum L.). The relative heat tolerance of nine potato cultivars grown under greenhouse conditions was determined using the electrolyte leakage assay (ELA), a standard quantitative assay for heat tolerance. Three cultivars differing in heat tolerance were selected and designated as heat-tolerant (‘Laura’), moderately sensitive (‘Liseta’) and heat-sensitive (‘Agria’) genotypes. The expression of cytosolic HSP18 and chloroplast HSP21 was analyzed at the protein level in the leaves of selected cultivars, both ex vitro- and in vitro-grown, after heat stress or control treatment. Immunoblot analysis revealed heat-induced HSP18 and HSP21 expression in all examined genotypes. A similar pattern of examined sHSP expression was observed ex vitro and in vitro: heat-tolerant ‘Laura’ accumulated higher levels of both HSP18 and HSP21 compared to heat-sensitive ‘Liseta’ and ‘Agria’. Our results indicate that ELA combined with immunoblot analysis of sHSP accumulation under HS conditions, might be considered as a reliable procedure in screening potato genotypes for heat tolerance. To our knowledge, this is the first study where sHSP expression between ex vitro- and in vitro-grown potato plants was compared.",
journal = "Archives of Biological Sciences",
title = "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)",
number = "1",
volume = "64",
pages = "135-144",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_298"
}

Savić, J., Dragićević, I., Pantelić, D., Oljača, J.,& Momčilović, I.. (2012). Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences, 64(1), 135-144.
https://hdl.handle.net/21.15107/rcub_ibiss_298

Savić J, Dragićević I, Pantelić D, Oljača J, Momčilović I. Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.). in Archives of Biological Sciences. 2012;64(1):135-144.
https://hdl.handle.net/21.15107/rcub_ibiss_298 .

Savić, Jelena, Dragićević, Ivana, Pantelić, D., Oljača, Jasmina, Momčilović, Ivana, "Expression of small heat shock proteins and heat tolerance in potato (Solanum tuberosum L.)" in Archives of Biological Sciences, 64, no. 1 (2012):135-144,
https://hdl.handle.net/21.15107/rcub_ibiss_298 .

TY  - JOUR
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana
AU  - Zdravković-Korać, Snežana
AU  - Dragićević, Ivana
AU  - Ćalić, Dušica
AU  - Vinterhalter, Branka
PY  - 2011
UR  - S0304423811004468
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6108
AB  - Somatic embryos (SEs) were induced from apical sections of the lateral roots of spinach seedlings (1 cm), which were cultivated on solid Murashige and Skoog (MS) medium with 20 μM α-naphthaleneacetic acid and 5 μM gibberellic acid. Apical shoots of the same lines were isolated and cultivated on plant growth regulator-free medium. The regeneration capacities of seedlings randomly chosen from a population were quite low and variable, and only 4 out of 30 lines responded at the frequency of 85–100%, with 6.96–9.96 SEs per explant and up to 347 SEs per seedling over a 12-week period. These SEs were isolated and maintained on medium with 5 μM kinetin. Plants derived from seedlings’ apical shoot and SEs selffertilised in vitro and set seeds, and these seedlings (S1) were used to induce regeneration. Similarly, S2–S4 seedlings were obtained and the regeneration capacities of 23 S1, 23 S2, 17 S3 and 5 S4-seedlings were compared to parental lines. Of these, four S3 and S4 lines with extremely high regeneration capacities were selected. These lines exhibited 78–139 fold higher embryo-forming capacities than the mother plant, and produced 38.9–68.3 SEs per explant and 1339–2181 SEs per seedling during the same time period. In addition, the process of somatic embryogenesis began 2–4 weeks earlier in these lines, and root explants taken from SE-derived plants of these lines retained high and stable regeneration capacities, and therefore may be ideal material for genetic transformation.
PB  - Amsterdam: Elsevier
T2  - Scientia Horticulturae
T1  - Increased regeneration capacity in spinach lines obtained by in vitro self-fertilisation
IS  - 4
VL  - 130
DO  - 10.1016/j.scienta.2011.08.024
SP  - 681
EP  - 690
ER  - 

@article{
author = "Milojević, Jelena and Tubić, Ljiljana and Zdravković-Korać, Snežana and Dragićević, Ivana and Ćalić, Dušica and Vinterhalter, Branka",
year = "2011",
abstract = "Somatic embryos (SEs) were induced from apical sections of the lateral roots of spinach seedlings (1 cm), which were cultivated on solid Murashige and Skoog (MS) medium with 20 μM α-naphthaleneacetic acid and 5 μM gibberellic acid. Apical shoots of the same lines were isolated and cultivated on plant growth regulator-free medium. The regeneration capacities of seedlings randomly chosen from a population were quite low and variable, and only 4 out of 30 lines responded at the frequency of 85–100%, with 6.96–9.96 SEs per explant and up to 347 SEs per seedling over a 12-week period. These SEs were isolated and maintained on medium with 5 μM kinetin. Plants derived from seedlings’ apical shoot and SEs selffertilised in vitro and set seeds, and these seedlings (S1) were used to induce regeneration. Similarly, S2–S4 seedlings were obtained and the regeneration capacities of 23 S1, 23 S2, 17 S3 and 5 S4-seedlings were compared to parental lines. Of these, four S3 and S4 lines with extremely high regeneration capacities were selected. These lines exhibited 78–139 fold higher embryo-forming capacities than the mother plant, and produced 38.9–68.3 SEs per explant and 1339–2181 SEs per seedling during the same time period. In addition, the process of somatic embryogenesis began 2–4 weeks earlier in these lines, and root explants taken from SE-derived plants of these lines retained high and stable regeneration capacities, and therefore may be ideal material for genetic transformation.",
publisher = "Amsterdam: Elsevier",
journal = "Scientia Horticulturae",
title = "Increased regeneration capacity in spinach lines obtained by in vitro self-fertilisation",
number = "4",
volume = "130",
doi = "10.1016/j.scienta.2011.08.024",
pages = "681-690"
}

Milojević, J., Tubić, L., Zdravković-Korać, S., Dragićević, I., Ćalić, D.,& Vinterhalter, B.. (2011). Increased regeneration capacity in spinach lines obtained by in vitro self-fertilisation. in Scientia Horticulturae
Amsterdam: Elsevier., 130(4), 681-690.
https://doi.org/10.1016/j.scienta.2011.08.024

Milojević J, Tubić L, Zdravković-Korać S, Dragićević I, Ćalić D, Vinterhalter B. Increased regeneration capacity in spinach lines obtained by in vitro self-fertilisation. in Scientia Horticulturae. 2011;130(4):681-690.
doi:10.1016/j.scienta.2011.08.024 .

Milojević, Jelena, Tubić, Ljiljana, Zdravković-Korać, Snežana, Dragićević, Ivana, Ćalić, Dušica, Vinterhalter, Branka, "Increased regeneration capacity in spinach lines obtained by in vitro self-fertilisation" in Scientia Horticulturae, 130, no. 4 (2011):681-690,
https://doi.org/10.1016/j.scienta.2011.08.024 . .

TY  - JOUR
AU  - Ćalić-Dragosavac, Dušica D.
AU  - Pemac, Danijela
AU  - Dragićević, Ivana
AU  - Radojević, Ljiljana
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/187
T2  - Archives of Biological Sciences
T1  - Variability and bimodal distribution of size in uninuclear microspores of Aesculus flava Marshall
IS  - 1
VL  - 60
SP  - 3
EP  - 4
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_187
ER  - 

@article{
author = "Ćalić-Dragosavac, Dušica D. and Pemac, Danijela and Dragićević, Ivana and Radojević, Ljiljana",
year = "2008, 2008",
journal = "Archives of Biological Sciences",
title = "Variability and bimodal distribution of size in uninuclear microspores of Aesculus flava Marshall",
number = "1",
volume = "60",
pages = "3-4",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_187"
}

Ćalić-Dragosavac, D. D., Pemac, D., Dragićević, I.,& Radojević, L.. (2008). Variability and bimodal distribution of size in uninuclear microspores of Aesculus flava Marshall. in Archives of Biological Sciences, 60(1), 3-4.
https://hdl.handle.net/21.15107/rcub_ibiss_187

Ćalić-Dragosavac DD, Pemac D, Dragićević I, Radojević L. Variability and bimodal distribution of size in uninuclear microspores of Aesculus flava Marshall. in Archives of Biological Sciences. 2008;60(1):3-4.
https://hdl.handle.net/21.15107/rcub_ibiss_187 .

Ćalić-Dragosavac, Dušica D., Pemac, Danijela, Dragićević, Ivana, Radojević, Ljiljana, "Variability and bimodal distribution of size in uninuclear microspores of Aesculus flava Marshall" in Archives of Biological Sciences, 60, no. 1 (2008):3-4,
https://hdl.handle.net/21.15107/rcub_ibiss_187 .