TY  - CONF
AU  - Jović, Milena
AU  - Mladenović, Aleksandra
AU  - Milanović, Desanka
AU  - Todorović, Smilja
AU  - Prvulović, Milica
AU  - Vukojević, Anđela
AU  - Simeunović, Valentina
AU  - Sokanović, Srđan
AU  - Lončarević, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5323
AB  - The presence of large amounts of amyloid beta (Aβ) oligomers, amyloid plaques formation, and inflammation in the brain are one of the neuropathological characteristics
of Alzheimer's disease (AD). The use of supplements with omega­3 fatty acids has been associated with reduced risk and lessened AD pathology. The purpose of this
study was to elucidate whether such a treatment could affects glial cells and macrophages behavior in the early phase of the disease. 
We examined influence of fish oil (FO) treatment in 5xFAD mice, an animal model which rapidly recapitulates major hallmarks of AD amyloid pathology. Three­month
old female 5xFAD mice received FO (100μl/animal/day) via oral gavage during 3 weeks period. Histological analysis was used to detect changes in pathological
features of AD in parietal cortex of 5xFAD mice. Aβ peptide, macrophages, microglial cells and astrocytes were detected by anti­Aβ42­, anti­Iba­1, anti­TMEM119­
and anti­GFAP­antibody, respectively. Immunostaining was observed by confocal microscopy.  Quantification was done by ImageJ. FO supplementation alters the
behaviour of macrophages prompting them to establish a physical barrier around amyloid plaques and leads to changes in number of over all astrocytes and microglial
cells. 
These results confirmed and extended previous findings suggesting that FO supplementation suppresses brain aging and has a typical pleiotropic effect, suggesting that
FO in combination with other drugs could be good approach for long­term treatment in AD suppression.
PB  - Federation of European Biochemical Societies
C3  - Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece
T1  - Short term fish oil supplementation changes glial cells function in 5XFAD mice
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5323
ER  - 

@conference{
author = "Jović, Milena and Mladenović, Aleksandra and Milanović, Desanka and Todorović, Smilja and Prvulović, Milica and Vukojević, Anđela and Simeunović, Valentina and Sokanović, Srđan and Lončarević, Nataša",
year = "2022",
abstract = "The presence of large amounts of amyloid beta (Aβ) oligomers, amyloid plaques formation, and inflammation in the brain are one of the neuropathological characteristics
of Alzheimer's disease (AD). The use of supplements with omega­3 fatty acids has been associated with reduced risk and lessened AD pathology. The purpose of this
study was to elucidate whether such a treatment could affects glial cells and macrophages behavior in the early phase of the disease. 
We examined influence of fish oil (FO) treatment in 5xFAD mice, an animal model which rapidly recapitulates major hallmarks of AD amyloid pathology. Three­month
old female 5xFAD mice received FO (100μl/animal/day) via oral gavage during 3 weeks period. Histological analysis was used to detect changes in pathological
features of AD in parietal cortex of 5xFAD mice. Aβ peptide, macrophages, microglial cells and astrocytes were detected by anti­Aβ42­, anti­Iba­1, anti­TMEM119­
and anti­GFAP­antibody, respectively. Immunostaining was observed by confocal microscopy.  Quantification was done by ImageJ. FO supplementation alters the
behaviour of macrophages prompting them to establish a physical barrier around amyloid plaques and leads to changes in number of over all astrocytes and microglial
cells. 
These results confirmed and extended previous findings suggesting that FO supplementation suppresses brain aging and has a typical pleiotropic effect, suggesting that
FO in combination with other drugs could be good approach for long­term treatment in AD suppression.",
publisher = "Federation of European Biochemical Societies",
journal = "Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece",
title = "Short term fish oil supplementation changes glial cells function in 5XFAD mice",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5323"
}

Jović, M., Mladenović, A., Milanović, D., Todorović, S., Prvulović, M., Vukojević, A., Simeunović, V., Sokanović, S.,& Lončarević, N.. (2022). Short term fish oil supplementation changes glial cells function in 5XFAD mice. in Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece
Federation of European Biochemical Societies..
https://hdl.handle.net/21.15107/rcub_ibiss_5323

Jović M, Mladenović A, Milanović D, Todorović S, Prvulović M, Vukojević A, Simeunović V, Sokanović S, Lončarević N. Short term fish oil supplementation changes glial cells function in 5XFAD mice. in Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5323 .

Jović, Milena, Mladenović, Aleksandra, Milanović, Desanka, Todorović, Smilja, Prvulović, Milica, Vukojević, Anđela, Simeunović, Valentina, Sokanović, Srđan, Lončarević, Nataša, "Short term fish oil supplementation changes glial cells function in 5XFAD mice" in Book of Abstracts: Joint IUBMB/FEBS Advanced Lecture Course: Molecular targets for anti-aging interventions; 2022 Sep 26 - Oct 1; Spetses Island, Greece (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5323 .