TY  - JOUR
AU  - Rakić, Dejana
AU  - Joksimović Jović, Jovana
AU  - Jakovljević, Vladimir
AU  - Živković, Vladimir
AU  - Nikolić, Maja
AU  - Sretenović, Jasmina
AU  - Nikolić, Marina
AU  - Jović, Nikola
AU  - Bićanin Ilić, Marija
AU  - Arsenijević, Petar
AU  - Dimitrijević, Aleksandra
AU  - Vulović, Tatjana
AU  - Ristić, Nataša
AU  - Bulatović, Kristina
AU  - Bolevich, Sergej
AU  - Stijak, Lazar
AU  - Pantović, Suzana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6087
AB  - Polycystic ovary syndrome (PCOS) is a frequent multifactorial
endocrinopathy affecting women in the reproductive period, often associated with infertility and
metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling
the examination of the effects of certain drugs in order to discover the best possible therapeutic
approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet
(HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress.
Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiolvalerate
group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was
induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to
improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and
EV group had a regular diet, while the EV + HFD group had HFD during the induction period of
60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances,
along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose
metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone.
Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD
protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic
base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions:
The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our
study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.
PB  - Basel: MDPI
T2  - Medicina
T1  - High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats
IS  - 6
VL  - 59
DO  - 10.3390/medicina59061104
SP  - 1104
ER  - 

@article{
author = "Rakić, Dejana and Joksimović Jović, Jovana and Jakovljević, Vladimir and Živković, Vladimir and Nikolić, Maja and Sretenović, Jasmina and Nikolić, Marina and Jović, Nikola and Bićanin Ilić, Marija and Arsenijević, Petar and Dimitrijević, Aleksandra and Vulović, Tatjana and Ristić, Nataša and Bulatović, Kristina and Bolevich, Sergej and Stijak, Lazar and Pantović, Suzana",
year = "2023",
abstract = "Polycystic ovary syndrome (PCOS) is a frequent multifactorial
endocrinopathy affecting women in the reproductive period, often associated with infertility and
metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling
the examination of the effects of certain drugs in order to discover the best possible therapeutic
approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet
(HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress.
Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiolvalerate
group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was
induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to
improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and
EV group had a regular diet, while the EV + HFD group had HFD during the induction period of
60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances,
along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose
metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone.
Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD
protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic
base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions:
The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our
study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.",
publisher = "Basel: MDPI",
journal = "Medicina",
title = "High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats",
number = "6",
volume = "59",
doi = "10.3390/medicina59061104",
pages = "1104"
}

Rakić, D., Joksimović Jović, J., Jakovljević, V., Živković, V., Nikolić, M., Sretenović, J., Nikolić, M., Jović, N., Bićanin Ilić, M., Arsenijević, P., Dimitrijević, A., Vulović, T., Ristić, N., Bulatović, K., Bolevich, S., Stijak, L.,& Pantović, S.. (2023). High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats. in Medicina
Basel: MDPI., 59(6), 1104.
https://doi.org/10.3390/medicina59061104

Rakić D, Joksimović Jović J, Jakovljević V, Živković V, Nikolić M, Sretenović J, Nikolić M, Jović N, Bićanin Ilić M, Arsenijević P, Dimitrijević A, Vulović T, Ristić N, Bulatović K, Bolevich S, Stijak L, Pantović S. High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats. in Medicina. 2023;59(6):1104.
doi:10.3390/medicina59061104 .

Rakić, Dejana, Joksimović Jović, Jovana, Jakovljević, Vladimir, Živković, Vladimir, Nikolić, Maja, Sretenović, Jasmina, Nikolić, Marina, Jović, Nikola, Bićanin Ilić, Marija, Arsenijević, Petar, Dimitrijević, Aleksandra, Vulović, Tatjana, Ristić, Nataša, Bulatović, Kristina, Bolevich, Sergej, Stijak, Lazar, Pantović, Suzana, "High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats" in Medicina, 59, no. 6 (2023):1104,
https://doi.org/10.3390/medicina59061104 . .

TY  - JOUR
AU  - Joksimović Jović, Jovana
AU  - Jović, Nikola
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Nikolić, Maja
AU  - Rudić, Jovan
AU  - Milošević, Verica
AU  - Ristić, Nataša
AU  - Andrić, Kristina
AU  - Dimkić Tomić, Tijana
AU  - Miličić, Biljana
AU  - Jakovljević, Vladimir
PY  - 2021
UR  - https://rep.bioscientifica.com/view/journals/rep/163/1/REP-21-0219.xml
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4771
AB  - Numerous evidence implies complex interrelations between polycystic ovary syndrome (PCOS) and hypertension (HT) in reproductive-age women. In this study, we aimed to investigate the potential strain differences in ovarian morphology, hemodynamic, and biochemical characteristics in an androgen-induced PCOS rat model. A total of 24 rats of 3 weeks old (12 Wistar Kyoto - WK and 12 spontaneously hypertensive rats - SHR) were divided into four groups: WK, WK PCOS, SHR, and SHR PCOS. PCOS was induced by daily s.c. injections of testosterone enanthate (1 mg/100 g body weight) administered for 5 weeks. PCOS induction led to estrus cyclicity cessation, cystic ovarian appearance, and sex hormones disturbances in both strains. The morphometric parameters in ovaries were altered in a manner of PCOS-related changes in both strains (higher number in preantral, atretic, and cystic follicles). Ultrasonographically, a significant decrease in ovarian volume (OV) was registered in PCOS groups but also in SHR compared to WK rats. All blood pressure parameters were higher in SHR compared to WK. PCOS modeling increased systolic, mean arterial, and pulse pressure in WK strain, while in SHR, only mean arterial and pulse pressure were higher. Alterations in oxidative stress parameters could provide a molecular basis for PCOS-related changes: in PCOS groups, thiobarbituric acid reactive substance and superoxide anion radical levels were higher in both strains, while superoxide dismutase and glutathione were significantly lowered.
PB  - Society For Reproduction And Fertility
T2  - Reproduction (Cambridge, England)
T1  - Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.
IS  - 1
VL  - 163
DO  - 10.1530/REP-21-0219
SP  - 11
EP  - 21
ER  - 

@article{
author = "Joksimović Jović, Jovana and Jović, Nikola and Sretenović, Jasmina and Živković, Vladimir and Nikolić, Maja and Rudić, Jovan and Milošević, Verica and Ristić, Nataša and Andrić, Kristina and Dimkić Tomić, Tijana and Miličić, Biljana and Jakovljević, Vladimir",
year = "2021",
abstract = "Numerous evidence implies complex interrelations between polycystic ovary syndrome (PCOS) and hypertension (HT) in reproductive-age women. In this study, we aimed to investigate the potential strain differences in ovarian morphology, hemodynamic, and biochemical characteristics in an androgen-induced PCOS rat model. A total of 24 rats of 3 weeks old (12 Wistar Kyoto - WK and 12 spontaneously hypertensive rats - SHR) were divided into four groups: WK, WK PCOS, SHR, and SHR PCOS. PCOS was induced by daily s.c. injections of testosterone enanthate (1 mg/100 g body weight) administered for 5 weeks. PCOS induction led to estrus cyclicity cessation, cystic ovarian appearance, and sex hormones disturbances in both strains. The morphometric parameters in ovaries were altered in a manner of PCOS-related changes in both strains (higher number in preantral, atretic, and cystic follicles). Ultrasonographically, a significant decrease in ovarian volume (OV) was registered in PCOS groups but also in SHR compared to WK rats. All blood pressure parameters were higher in SHR compared to WK. PCOS modeling increased systolic, mean arterial, and pulse pressure in WK strain, while in SHR, only mean arterial and pulse pressure were higher. Alterations in oxidative stress parameters could provide a molecular basis for PCOS-related changes: in PCOS groups, thiobarbituric acid reactive substance and superoxide anion radical levels were higher in both strains, while superoxide dismutase and glutathione were significantly lowered.",
publisher = "Society For Reproduction And Fertility",
journal = "Reproduction (Cambridge, England)",
title = "Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.",
number = "1",
volume = "163",
doi = "10.1530/REP-21-0219",
pages = "11-21"
}

Joksimović Jović, J., Jović, N., Sretenović, J., Živković, V., Nikolić, M., Rudić, J., Milošević, V., Ristić, N., Andrić, K., Dimkić Tomić, T., Miličić, B.,& Jakovljević, V.. (2021). Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.. in Reproduction (Cambridge, England)
Society For Reproduction And Fertility., 163(1), 11-21.
https://doi.org/10.1530/REP-21-0219

Joksimović Jović J, Jović N, Sretenović J, Živković V, Nikolić M, Rudić J, Milošević V, Ristić N, Andrić K, Dimkić Tomić T, Miličić B, Jakovljević V. Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress.. in Reproduction (Cambridge, England). 2021;163(1):11-21.
doi:10.1530/REP-21-0219 .

Joksimović Jović, Jovana, Jović, Nikola, Sretenović, Jasmina, Živković, Vladimir, Nikolić, Maja, Rudić, Jovan, Milošević, Verica, Ristić, Nataša, Andrić, Kristina, Dimkić Tomić, Tijana, Miličić, Biljana, Jakovljević, Vladimir, "Normotensive rats with PCOS exhibit the hypertensive pattern: focus on oxidative stress." in Reproduction (Cambridge, England), 163, no. 1 (2021):11-21,
https://doi.org/10.1530/REP-21-0219 . .