TY  - JOUR
AU  - Rakić, Dejana
AU  - Joksimović Jović, Jovana
AU  - Jakovljević, Vladimir
AU  - Živković, Vladimir
AU  - Nikolić, Maja
AU  - Sretenović, Jasmina
AU  - Nikolić, Marina
AU  - Jović, Nikola
AU  - Bićanin Ilić, Marija
AU  - Arsenijević, Petar
AU  - Dimitrijević, Aleksandra
AU  - Vulović, Tatjana
AU  - Ristić, Nataša
AU  - Bulatović, Kristina
AU  - Bolevich, Sergej
AU  - Stijak, Lazar
AU  - Pantović, Suzana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6087
AB  - Polycystic ovary syndrome (PCOS) is a frequent multifactorial
endocrinopathy affecting women in the reproductive period, often associated with infertility and
metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling
the examination of the effects of certain drugs in order to discover the best possible therapeutic
approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet
(HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress.
Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiolvalerate
group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was
induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to
improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and
EV group had a regular diet, while the EV + HFD group had HFD during the induction period of
60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances,
along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose
metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone.
Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD
protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic
base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions:
The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our
study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.
PB  - Basel: MDPI
T2  - Medicina
T1  - High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats
IS  - 6
VL  - 59
DO  - 10.3390/medicina59061104
SP  - 1104
ER  - 

@article{
author = "Rakić, Dejana and Joksimović Jović, Jovana and Jakovljević, Vladimir and Živković, Vladimir and Nikolić, Maja and Sretenović, Jasmina and Nikolić, Marina and Jović, Nikola and Bićanin Ilić, Marija and Arsenijević, Petar and Dimitrijević, Aleksandra and Vulović, Tatjana and Ristić, Nataša and Bulatović, Kristina and Bolevich, Sergej and Stijak, Lazar and Pantović, Suzana",
year = "2023",
abstract = "Polycystic ovary syndrome (PCOS) is a frequent multifactorial
endocrinopathy affecting women in the reproductive period, often associated with infertility and
metabolic disorders. The use of animal models helps to better understand etiopathogenesis, enabling
the examination of the effects of certain drugs in order to discover the best possible therapeutic
approach. We tried to investigate the additional effect of estradiol-valerate (EV) and high-fat diet
(HFD) in female rats to explore PCOS-related alterations with special focus on oxidative stress.
Materials and Methods: Animals were divided into three groups: control group (CTRL, n = 6), estradiolvalerate
group (EV, n = 6), and estradiol-valerate group on HFD (EV + HFD, n = 6). PCOS was
induced by single subcutaneous injection of long-acting EV in a dose of 4 mg/per rat. We tried to
improve the metabolic characteristics of the PCOS animal model by adding HFD, so the CTRL and
EV group had a regular diet, while the EV + HFD group had HFD during the induction period of
60 days. Results: We observed alterations of anthropometric parameters and hormonal disturbances,
along with estrus cycle impairment reassembly to obese-type PCOS phenotype. Moreover, glucose
metabolism was impaired after addition of HFD to EV protocol, contrary to EV administered alone.
Histological analysis confirmed more numerous cystic follicles after the combination of EV and HFD
protocol. The alterations of oxidative stress markers could be related to and serve as the mechanistic
base for development of PCOS-related endocrine, reproductive, and metabolic properties. Conclusions:
The additive effect of EV and HFD was obvious in the majority of the parameters observed. Our
study strongly demonstrated metabolic as well as reproductive properties of PCOS in rats.",
publisher = "Basel: MDPI",
journal = "Medicina",
title = "High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats",
number = "6",
volume = "59",
doi = "10.3390/medicina59061104",
pages = "1104"
}

Rakić, D., Joksimović Jović, J., Jakovljević, V., Živković, V., Nikolić, M., Sretenović, J., Nikolić, M., Jović, N., Bićanin Ilić, M., Arsenijević, P., Dimitrijević, A., Vulović, T., Ristić, N., Bulatović, K., Bolevich, S., Stijak, L.,& Pantović, S.. (2023). High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats. in Medicina
Basel: MDPI., 59(6), 1104.
https://doi.org/10.3390/medicina59061104

Rakić D, Joksimović Jović J, Jakovljević V, Živković V, Nikolić M, Sretenović J, Nikolić M, Jović N, Bićanin Ilić M, Arsenijević P, Dimitrijević A, Vulović T, Ristić N, Bulatović K, Bolevich S, Stijak L, Pantović S. High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats. in Medicina. 2023;59(6):1104.
doi:10.3390/medicina59061104 .

Rakić, Dejana, Joksimović Jović, Jovana, Jakovljević, Vladimir, Živković, Vladimir, Nikolić, Maja, Sretenović, Jasmina, Nikolić, Marina, Jović, Nikola, Bićanin Ilić, Marija, Arsenijević, Petar, Dimitrijević, Aleksandra, Vulović, Tatjana, Ristić, Nataša, Bulatović, Kristina, Bolevich, Sergej, Stijak, Lazar, Pantović, Suzana, "High Fat Diet Exaggerate Metabolic and Reproductive PCOS Features by Promoting Oxidative Stress: An Improved EV Model in Rats" in Medicina, 59, no. 6 (2023):1104,
https://doi.org/10.3390/medicina59061104 . .

TY  - JOUR
AU  - Sretenović, Jasmina
AU  - Živković, Vladimir
AU  - Srejović, Ivan
AU  - Ajdžanović, Vladimir
AU  - Ristić, Nataša
AU  - Trifunović, Miroljub
AU  - Pantović, Suzana
AU  - Jović, Slavoljub
AU  - Jakovljević, Vladimir
AU  - Bolevich, Sergey
AU  - Milosavljević, Zoran
AU  - Milošević, Verica
PY  - 2020
UR  - /core/journals/microscopy-and-microanalysis/article/immunohistomorphometric-and-hormonal-analysis-of-the-pituitary-gonadotropic-cells-after-application-of-the-nandrolone-decanoate-and-swimming-training-in-adult-male-rats/CB060C51EF744799F8CC8590E060CAB8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3717
AB  - The aim of the study was to investigate the effects of chronic nandrolone decanoate treatment and/or swimming training on immunohistomorphometric parameters on rat pituitary gonadotropic cells. Male Wistar albino rats, 10 weeks old, were classified into four groups: control (T−N−), nandrolone (T−N+), swimming training (T+N−), and swimming training with nandrolone (T+N+). The T+ groups swam for 4 weeks, 1 h/day, 5 days/week. The N+ groups received nandrolone decanoate (20 mg/kg) once per week for 4 weeks. Pituitary tissue sections were processed and stained for immunohistochemical analysis and immunofluorescence. The volume density of luteinizing hormone (LH) cells was decreased by 48% in T−N+ and for 35% in the T+N+ group. The volume density of follicle-stimulating hormone (FSH) cells was decreased by 39% in T−N+ and for 30% in T+N+ compared to the control. Nandrolone alone, or combined with swimming training, decreased the number of LH/FSH cells compared to the control. The levels of the immunofluorescent signal of LH/FSH cells were increased in all experimental groups. Nandrolone alone decreased the serum level of LH by 17%, whereas swimming training alone increased FSH levels by 11% compared to the control. Serum levels of testosterone were increased in all experimental groups. Nandrolone alone, or combined with swimming training, decreased immunohistomorphometric parameters of gonadotropic cells, whereas the levels of immunofluorescent signal were increased.
PB  - Cambridge University Press (CUP)
T2  - Microscopy and Microanalysis
T1  - Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats
IS  - 4
VL  - 26
DO  - 10.1017/s143192762000166x
SP  - 699
EP  - 707
ER  - 

@article{
author = "Sretenović, Jasmina and Živković, Vladimir and Srejović, Ivan and Ajdžanović, Vladimir and Ristić, Nataša and Trifunović, Miroljub and Pantović, Suzana and Jović, Slavoljub and Jakovljević, Vladimir and Bolevich, Sergey and Milosavljević, Zoran and Milošević, Verica",
year = "2020",
abstract = "The aim of the study was to investigate the effects of chronic nandrolone decanoate treatment and/or swimming training on immunohistomorphometric parameters on rat pituitary gonadotropic cells. Male Wistar albino rats, 10 weeks old, were classified into four groups: control (T−N−), nandrolone (T−N+), swimming training (T+N−), and swimming training with nandrolone (T+N+). The T+ groups swam for 4 weeks, 1 h/day, 5 days/week. The N+ groups received nandrolone decanoate (20 mg/kg) once per week for 4 weeks. Pituitary tissue sections were processed and stained for immunohistochemical analysis and immunofluorescence. The volume density of luteinizing hormone (LH) cells was decreased by 48% in T−N+ and for 35% in the T+N+ group. The volume density of follicle-stimulating hormone (FSH) cells was decreased by 39% in T−N+ and for 30% in T+N+ compared to the control. Nandrolone alone, or combined with swimming training, decreased the number of LH/FSH cells compared to the control. The levels of the immunofluorescent signal of LH/FSH cells were increased in all experimental groups. Nandrolone alone decreased the serum level of LH by 17%, whereas swimming training alone increased FSH levels by 11% compared to the control. Serum levels of testosterone were increased in all experimental groups. Nandrolone alone, or combined with swimming training, decreased immunohistomorphometric parameters of gonadotropic cells, whereas the levels of immunofluorescent signal were increased.",
publisher = "Cambridge University Press (CUP)",
journal = "Microscopy and Microanalysis",
title = "Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats",
number = "4",
volume = "26",
doi = "10.1017/s143192762000166x",
pages = "699-707"
}

Sretenović, J., Živković, V., Srejović, I., Ajdžanović, V., Ristić, N., Trifunović, M., Pantović, S., Jović, S., Jakovljević, V., Bolevich, S., Milosavljević, Z.,& Milošević, V.. (2020). Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats. in Microscopy and Microanalysis
Cambridge University Press (CUP)., 26(4), 699-707.
https://doi.org/10.1017/s143192762000166x

Sretenović J, Živković V, Srejović I, Ajdžanović V, Ristić N, Trifunović M, Pantović S, Jović S, Jakovljević V, Bolevich S, Milosavljević Z, Milošević V. Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats. in Microscopy and Microanalysis. 2020;26(4):699-707.
doi:10.1017/s143192762000166x .

Sretenović, Jasmina, Živković, Vladimir, Srejović, Ivan, Ajdžanović, Vladimir, Ristić, Nataša, Trifunović, Miroljub, Pantović, Suzana, Jović, Slavoljub, Jakovljević, Vladimir, Bolevich, Sergey, Milosavljević, Zoran, Milošević, Verica, "Immunohistomorphometric and Hormonal Analysis of the Pituitary Gonadotropic Cells After Application of the Nandrolone Decanoate and Swimming Training in Adult Male Rats" in Microscopy and Microanalysis, 26, no. 4 (2020):699-707,
https://doi.org/10.1017/s143192762000166x . .