TY  - JOUR
AU  - Vranković, Jelena
AU  - Slavic, Marija
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2096
AB  - PCBs are potential genotoxicants and inductors of increased levels of
   ROS and oxidative stress in both humans and wildlife. In this study,
   biotransformation parameters and antioxidant (AO) enzymes were analyzed
   in the Asian clam, Corbicula fluminea, with respect to the presence of
   dioxin-like PCBs (dl-PCBs) in the environment. For this purpose, clams
   were collected from three localities with different environmental
   conditions. Sediments from each locality and clam tissues were analyzed
   for the presence of dl-PCBs. Biological responses included changes in
   the expression of fish CYP1A immunoreactive protein, changes in the
   activities of AO enzymes, SOD, CAT, GR and GPx, and the phase II
   biotransformation enzyme GST. Clams from the locality with the highest
   EWHO TEQ values for sediment dl-PCBs had the highest EWHO TEQ values for
   tissue dl-PCBs and the highest level of fish CYP1A immunoreactive
   protein expression. The correlations found between the concentrations of
   dl-PCBs and AO and GST activities revealed both concentration-dependent
   inhibitory and stimulatory effects. The activities of GPx, GR and GST
   were very sensitive to environmental xenobiotics, including dl-PCBs. The
   obtained results indicate that the activities of these enzymes were
   fine-tuned to provide optimized antioxidant responses. We also observed
   seasonal changes in GST and AO enzyme activities in Asian clam. The
   highest levels of activity were observed in spring as compared to summer
   or autumn. CDA, which was performed in order to classify the different
   clam populations, revealed a coordinated rearrangement of the clams'
   metabolic components that were probably aimed at compensating for
   environmental fluctuations. The presented data reveal the sensitivity of
   C. fiuminea to the presence of dl-PCBs in the environment and support
   the use of this clam as a sentinel species for biomonitoring studies.
   (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Ecological Indicators
T1  - Biomarker responses in Corbicula fluminea to the presence of dioxin-like
 polychlorinated biphenyls and seasonal changes
VL  - 48
DO  - 10.1016/j.ecolind.2014.08.005
SP  - 99
EP  - 106
ER  - 

@article{
author = "Vranković, Jelena and Slavic, Marija",
year = "2015",
abstract = "PCBs are potential genotoxicants and inductors of increased levels of
   ROS and oxidative stress in both humans and wildlife. In this study,
   biotransformation parameters and antioxidant (AO) enzymes were analyzed
   in the Asian clam, Corbicula fluminea, with respect to the presence of
   dioxin-like PCBs (dl-PCBs) in the environment. For this purpose, clams
   were collected from three localities with different environmental
   conditions. Sediments from each locality and clam tissues were analyzed
   for the presence of dl-PCBs. Biological responses included changes in
   the expression of fish CYP1A immunoreactive protein, changes in the
   activities of AO enzymes, SOD, CAT, GR and GPx, and the phase II
   biotransformation enzyme GST. Clams from the locality with the highest
   EWHO TEQ values for sediment dl-PCBs had the highest EWHO TEQ values for
   tissue dl-PCBs and the highest level of fish CYP1A immunoreactive
   protein expression. The correlations found between the concentrations of
   dl-PCBs and AO and GST activities revealed both concentration-dependent
   inhibitory and stimulatory effects. The activities of GPx, GR and GST
   were very sensitive to environmental xenobiotics, including dl-PCBs. The
   obtained results indicate that the activities of these enzymes were
   fine-tuned to provide optimized antioxidant responses. We also observed
   seasonal changes in GST and AO enzyme activities in Asian clam. The
   highest levels of activity were observed in spring as compared to summer
   or autumn. CDA, which was performed in order to classify the different
   clam populations, revealed a coordinated rearrangement of the clams'
   metabolic components that were probably aimed at compensating for
   environmental fluctuations. The presented data reveal the sensitivity of
   C. fiuminea to the presence of dl-PCBs in the environment and support
   the use of this clam as a sentinel species for biomonitoring studies.
   (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Ecological Indicators",
title = "Biomarker responses in Corbicula fluminea to the presence of dioxin-like
 polychlorinated biphenyls and seasonal changes",
volume = "48",
doi = "10.1016/j.ecolind.2014.08.005",
pages = "99-106"
}

Vranković, J.,& Slavic, M.. (2015). Biomarker responses in Corbicula fluminea to the presence of dioxin-like
 polychlorinated biphenyls and seasonal changes. in Ecological Indicators, 48, 99-106.
https://doi.org/10.1016/j.ecolind.2014.08.005

Vranković J, Slavic M. Biomarker responses in Corbicula fluminea to the presence of dioxin-like
 polychlorinated biphenyls and seasonal changes. in Ecological Indicators. 2015;48:99-106.
doi:10.1016/j.ecolind.2014.08.005 .

Vranković, Jelena, Slavic, Marija, "Biomarker responses in Corbicula fluminea to the presence of dioxin-like
 polychlorinated biphenyls and seasonal changes" in Ecological Indicators, 48 (2015):99-106,
https://doi.org/10.1016/j.ecolind.2014.08.005 . .

TY  - JOUR
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Spasojevic, Ivan
AU  - Slavic, Marija
AU  - Mijuskovic, Ana
AU  - Paskulin, Roman
AU  - Miljevic, Cedo
AU  - Spasić, Mihajlo
AU  - Blagojević, Duško
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1970
AB  - Ethnopharmacological relevance: Ibogaine is a naturally occurring
   alkaloid with psychotropic and metabotropic effects, derived from the
   bark of the root of the West African Tabernanthe iboga plant. The tribes
   of Kongo basin have been using iboga as a stimulant, for medicinal
   purposes, and in rite of passage ceremonies, for centuries. Besides, it
   has been found that this drug has anti-addictive effects.
   Aim of the study: Previous studies have demonstrated that ibogaine
   changed the quantity of ATP and energy related enzymes as well as the
   activity of antioxidant enzymes in cells thus altering redox equilibrium
   in a time manner. In this work, the mechanism of its action was further
   studied by measuring the effects of ibogaine in human erythrocytes in
   vitro on ATP liberation, membrane fluidity and antioxidant enzymes
   activity.
   Materials and methods: Heparinized human blood samples were incubated
   with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was
   separated by centrifugation and the levels of ATP and uric acid were
   measured 10 mm after the addition of ibogaine using standard kits. The
   activity of copper zinc superoxide dismutase (SOD1), catalase (CAT),
   glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were
   measured in erythrocytes after incubation period. The stability of SOD1
   activity was further tested through in vitro incubation with H2O2 and
   scanning of its electrophoretic profiles. Membrane fluidity was
   determined using an electron paramagnetic resonance spin-labelling
   method.
   Results: Results showed that ibogaine treatment of erythrocytes in vitro
   increased ATP concentration in the blood plasma without changes in
   neither erythrocytes membrane fluidity nor uric acid concentration.
   lbogaine also increased SOD1 activity in erythrocytes at both doses
   applied here. Treatment with 20 mu M also elevated GR activity after in
   vitro incubation at 37 degrees C. Electrophoretic profiles revealed that
   incubation with ibogaine mitigates H2O2 mediated suppression of SOD1
   activity.
   Conclusion: Some of the effects of ibogaine seem to be mediated through
   its influence on energy metabolism, redox active processes and the
   effects of discrete fluctuations of individual reactive oxygen species
   on different levels of enzyme activities. Overall, ibogaine acts as a
   pro-antioxidant by increasing activity of antioxidative enzymes and as
   an adaptagene in oxidative distress. (C) 2015 Published by Elsevier
   Ireland Ltd.
T2  - Journal of Ethnopharmacology
T1  - Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes
VL  - 164
DO  - 10.1016/j.jep.2015.01.037
SP  - 64
EP  - 70
ER  - 

@article{
author = "Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Spasojevic, Ivan and Slavic, Marija and Mijuskovic, Ana and Paskulin, Roman and Miljevic, Cedo and Spasić, Mihajlo and Blagojević, Duško",
year = "2015",
abstract = "Ethnopharmacological relevance: Ibogaine is a naturally occurring
   alkaloid with psychotropic and metabotropic effects, derived from the
   bark of the root of the West African Tabernanthe iboga plant. The tribes
   of Kongo basin have been using iboga as a stimulant, for medicinal
   purposes, and in rite of passage ceremonies, for centuries. Besides, it
   has been found that this drug has anti-addictive effects.
   Aim of the study: Previous studies have demonstrated that ibogaine
   changed the quantity of ATP and energy related enzymes as well as the
   activity of antioxidant enzymes in cells thus altering redox equilibrium
   in a time manner. In this work, the mechanism of its action was further
   studied by measuring the effects of ibogaine in human erythrocytes in
   vitro on ATP liberation, membrane fluidity and antioxidant enzymes
   activity.
   Materials and methods: Heparinized human blood samples were incubated
   with ibogaine (10 and 20 mu M) at 37 degrees C for 1 h. Blood plasma was
   separated by centrifugation and the levels of ATP and uric acid were
   measured 10 mm after the addition of ibogaine using standard kits. The
   activity of copper zinc superoxide dismutase (SOD1), catalase (CAT),
   glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were
   measured in erythrocytes after incubation period. The stability of SOD1
   activity was further tested through in vitro incubation with H2O2 and
   scanning of its electrophoretic profiles. Membrane fluidity was
   determined using an electron paramagnetic resonance spin-labelling
   method.
   Results: Results showed that ibogaine treatment of erythrocytes in vitro
   increased ATP concentration in the blood plasma without changes in
   neither erythrocytes membrane fluidity nor uric acid concentration.
   lbogaine also increased SOD1 activity in erythrocytes at both doses
   applied here. Treatment with 20 mu M also elevated GR activity after in
   vitro incubation at 37 degrees C. Electrophoretic profiles revealed that
   incubation with ibogaine mitigates H2O2 mediated suppression of SOD1
   activity.
   Conclusion: Some of the effects of ibogaine seem to be mediated through
   its influence on energy metabolism, redox active processes and the
   effects of discrete fluctuations of individual reactive oxygen species
   on different levels of enzyme activities. Overall, ibogaine acts as a
   pro-antioxidant by increasing activity of antioxidative enzymes and as
   an adaptagene in oxidative distress. (C) 2015 Published by Elsevier
   Ireland Ltd.",
journal = "Journal of Ethnopharmacology",
title = "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes",
volume = "164",
doi = "10.1016/j.jep.2015.01.037",
pages = "64-70"
}

Nikolić-Kokić, A., Oreščanin Dušić, Z., Spasojevic, I., Slavic, M., Mijuskovic, A., Paskulin, R., Miljevic, C., Spasić, M.,& Blagojević, D.. (2015). Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes. in Journal of Ethnopharmacology, 164, 64-70.
https://doi.org/10.1016/j.jep.2015.01.037

Nikolić-Kokić A, Oreščanin Dušić Z, Spasojevic I, Slavic M, Mijuskovic A, Paskulin R, Miljevic C, Spasić M, Blagojević D. Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes. in Journal of Ethnopharmacology. 2015;164:64-70.
doi:10.1016/j.jep.2015.01.037 .

Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Spasojevic, Ivan, Slavic, Marija, Mijuskovic, Ana, Paskulin, Roman, Miljevic, Cedo, Spasić, Mihajlo, Blagojević, Duško, "Ex vivo effects of ibogaine on the activity of antioxidative enzymes in
 human erythrocytes" in Journal of Ethnopharmacology, 164 (2015):64-70,
https://doi.org/10.1016/j.jep.2015.01.037 . .

TY  - CONF
AU  - Mijuskovic, Ana
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavic, Marija
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
AU  - Blagojević, Duško
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2211
C3  - Nitric Oxide-Biology and Chemistry
T1  - Sodium sulphide relaxation of rat uterus is related to calcium signaling
IS  - 1
VL  - 39
DO  - 10.1016/j.niox.2014.03.119
SP  - S36
EP  - S37
ER  - 

@conference{
author = "Mijuskovic, Ana and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavic, Marija and Spasić, Mihajlo and Spasojevic, Ivan and Blagojević, Duško",
year = "2014",
journal = "Nitric Oxide-Biology and Chemistry",
title = "Sodium sulphide relaxation of rat uterus is related to calcium signaling",
number = "1",
volume = "39",
doi = "10.1016/j.niox.2014.03.119",
pages = "S36-S37"
}

Mijuskovic, A., Oreščanin Dušić, Z., Nikolić-Kokić, A., Slavic, M., Spasić, M., Spasojevic, I.,& Blagojević, D.. (2014). Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry, 39(1), S36-S37.
https://doi.org/10.1016/j.niox.2014.03.119

Mijuskovic A, Oreščanin Dušić Z, Nikolić-Kokić A, Slavic M, Spasić M, Spasojevic I, Blagojević D. Sodium sulphide relaxation of rat uterus is related to calcium signaling. in Nitric Oxide-Biology and Chemistry. 2014;39(1):S36-S37.
doi:10.1016/j.niox.2014.03.119 .

Mijuskovic, Ana, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavic, Marija, Spasić, Mihajlo, Spasojevic, Ivan, Blagojević, Duško, "Sodium sulphide relaxation of rat uterus is related to calcium signaling" in Nitric Oxide-Biology and Chemistry, 39, no. 1 (2014):S36-S37,
https://doi.org/10.1016/j.niox.2014.03.119 . .

TY  - JOUR
AU  - Mijuskovic, Ana
AU  - Oreščanin Dušić, Zorana
AU  - Nikolić-Kokić, Aleksandra
AU  - Slavic, Marija
AU  - Spasić, Mihajlo
AU  - Spasojevic, Ivan
AU  - Blagojević, Duško
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2206
AB  - Background: Our aim was to investigate the effect of methanethiol
   (CH3SH) on contractility of rat uterus and activities of redox-active
   enzymes, and to compare them with the effect of sodium sulphide (Na2S),
   a hydrogen sulphide (H2S/HS-) donor.
   Methods: Uteri were isolated from virgin Wistar rats, divided into six
   groups, controls (untreated uteri allowed to contract spontaneously and
   in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and
   Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced).
   Underlying antioxidative enzyme activities (superoxide dismutase - SOD,
   glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or
   Na2S-treated uteri were compared to controls.
   Results: Our experiments showed that CH3SH and Na2S provoked reversible
   relaxation of both spontaneous and Ca2+ induced uterine contractions.
   The dose-response curves differed in shape, and CH3SH curve was shifted
   to higher concentration compared to H2S/HS-. The effects of Na2S fitted
   sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked
   increased SOD activity and decreased GR activity. However, Na2S
   (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+
   stimulated uteri, while the activity of GSHPx was increased in both
   types of active uteri.
   Conclusion: Our results imply that CH3SH may have a constructive role in
   the control of muscle function and metabolism. Observed differences
   between CH3SH and H2S/HS- could be attributed to a larger moiety that is
   present in CH3SH compared to H2S, but they are more likely to be a
   consequence of the specific actions of HS-, in relation to its negative
   charge. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences.
   Published by Elsevier Urban \& Partner Sp. Z o.o.. All rights reserved.
T2  - Pharmacological Reports
T1  - Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity
IS  - 3
VL  - 66
DO  - 10.1016/j.pharep.2013.12.012
SP  - 373
EP  - 379
ER  - 

@article{
author = "Mijuskovic, Ana and Oreščanin Dušić, Zorana and Nikolić-Kokić, Aleksandra and Slavic, Marija and Spasić, Mihajlo and Spasojevic, Ivan and Blagojević, Duško",
year = "2014",
abstract = "Background: Our aim was to investigate the effect of methanethiol
   (CH3SH) on contractility of rat uterus and activities of redox-active
   enzymes, and to compare them with the effect of sodium sulphide (Na2S),
   a hydrogen sulphide (H2S/HS-) donor.
   Methods: Uteri were isolated from virgin Wistar rats, divided into six
   groups, controls (untreated uteri allowed to contract spontaneously and
   in the presence of Ca2+(6 mM)), CH3SH treated (spontaneously active and
   Ca2+ induced) and Na2S treated (spontaneously active and Ca2+ induced).
   Underlying antioxidative enzyme activities (superoxide dismutase - SOD,
   glutathione peroxidase - GSHPx, glutathione reductase - GR) in CH3SH- or
   Na2S-treated uteri were compared to controls.
   Results: Our experiments showed that CH3SH and Na2S provoked reversible
   relaxation of both spontaneous and Ca2+ induced uterine contractions.
   The dose-response curves differed in shape, and CH3SH curve was shifted
   to higher concentration compared to H2S/HS-. The effects of Na2S fitted
   sigmoid curve, whereas those of CH3SH fitted linearly. CH3SH provoked
   increased SOD activity and decreased GR activity. However, Na2S
   (H2S/HS-) provoked an increase in SOD activity exclusively in Ca2+
   stimulated uteri, while the activity of GSHPx was increased in both
   types of active uteri.
   Conclusion: Our results imply that CH3SH may have a constructive role in
   the control of muscle function and metabolism. Observed differences
   between CH3SH and H2S/HS- could be attributed to a larger moiety that is
   present in CH3SH compared to H2S, but they are more likely to be a
   consequence of the specific actions of HS-, in relation to its negative
   charge. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences.
   Published by Elsevier Urban \& Partner Sp. Z o.o.. All rights reserved.",
journal = "Pharmacological Reports",
title = "Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity",
number = "3",
volume = "66",
doi = "10.1016/j.pharep.2013.12.012",
pages = "373-379"
}

Mijuskovic, A., Oreščanin Dušić, Z., Nikolić-Kokić, A., Slavic, M., Spasić, M., Spasojevic, I.,& Blagojević, D.. (2014). Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity. in Pharmacological Reports, 66(3), 373-379.
https://doi.org/10.1016/j.pharep.2013.12.012

Mijuskovic A, Oreščanin Dušić Z, Nikolić-Kokić A, Slavic M, Spasić M, Spasojevic I, Blagojević D. Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity. in Pharmacological Reports. 2014;66(3):373-379.
doi:10.1016/j.pharep.2013.12.012 .

Mijuskovic, Ana, Oreščanin Dušić, Zorana, Nikolić-Kokić, Aleksandra, Slavic, Marija, Spasić, Mihajlo, Spasojevic, Ivan, Blagojević, Duško, "Comparison of the effects of methanethiol and sodium sulphide on uterine
 contractile activity" in Pharmacological Reports, 66, no. 3 (2014):373-379,
https://doi.org/10.1016/j.pharep.2013.12.012 . .