TY  - JOUR
AU  - Djokic, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1984
AB  - Although numerous investigations have demonstrated a direct effect of
   cadmium (Cd) on peripheral blood mononuclear cell (PBMC) activity in
   humans, there is virtually no data concerning the in vivo impact of this
   metal on circulatory mononuclear cells. In this study, the effects of a
   sub-lethal Cd (1 mg/kg) dose were examined in rats 48 h following a
   single intraperitoneal injection. Cd treatment resulted in increased
   total peripheral blood leukocyte levels; however, decreases in PBMC
   numbers were seen. These changes coincided with an accumulation of
   mononuclear cells in the lungs and an increase in mononuclear cells
   expressing CD11b. A lack of effect of Cd on spontaneous nitric oxide
   (NO) production and on iNOS mRNA levels in the PBMC was also noted.
   Differential effects of Cd on PBMC inflammatory cytokine (IL-1 beta, TNF
   alpha, IL-6, IFN gamma, and IL-17) gene expression and production were
   also seen. Specifically, except for IL-1 beta (levels increased), there
   were decreases (relative to controls) in mRNA levels for all the other
   cytokines examined. While there were no Cd treatment-related changes in
   spontaneous production of the cytokines assessed, there seemed to be a
   trend (p = 0.06) toward a decrease in spontaneous IL-6 release. When
   these harvested cells were stimulated ex vivo, there was no effect from
   Cd exposure on LPS-stimulated IL-1 beta and TNF alpha or on
   ConA-stimulated IFN gamma or IL-17 production, but a decrease in IL-6
   production in response to LPS was, again, noted. A preliminary study
   with a lower Cd dose (0.5 mg/kg) revealed some of the same outcomes
   noted here (mononuclear cell infiltration into lungs, increases in PBMC
   IL-1 beta mRNA levels), but differential (increased IL-17 mRNA levels)
   or newly detected outcomes (increased levels of IL-1 alpha mRNA) as
   well. The described effects of the single in vivo exposure to Cd on PBMC
   might contribute to a better overall understanding of the
   immunomodulatory potential of this environmental contaminant.
T2  - Journal Of Immunotoxicology
T1  - Cadmium administration affects circulatory mononuclear cells in rats
IS  - 2
VL  - 12
DO  - 10.3109/1547691X.2014.904955
SP  - 115
EP  - 123
ER  - 

@article{
author = "Djokic, Jelena and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Mirkov, Ivana and Zolotarevski, Lidija and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2015",
abstract = "Although numerous investigations have demonstrated a direct effect of
   cadmium (Cd) on peripheral blood mononuclear cell (PBMC) activity in
   humans, there is virtually no data concerning the in vivo impact of this
   metal on circulatory mononuclear cells. In this study, the effects of a
   sub-lethal Cd (1 mg/kg) dose were examined in rats 48 h following a
   single intraperitoneal injection. Cd treatment resulted in increased
   total peripheral blood leukocyte levels; however, decreases in PBMC
   numbers were seen. These changes coincided with an accumulation of
   mononuclear cells in the lungs and an increase in mononuclear cells
   expressing CD11b. A lack of effect of Cd on spontaneous nitric oxide
   (NO) production and on iNOS mRNA levels in the PBMC was also noted.
   Differential effects of Cd on PBMC inflammatory cytokine (IL-1 beta, TNF
   alpha, IL-6, IFN gamma, and IL-17) gene expression and production were
   also seen. Specifically, except for IL-1 beta (levels increased), there
   were decreases (relative to controls) in mRNA levels for all the other
   cytokines examined. While there were no Cd treatment-related changes in
   spontaneous production of the cytokines assessed, there seemed to be a
   trend (p = 0.06) toward a decrease in spontaneous IL-6 release. When
   these harvested cells were stimulated ex vivo, there was no effect from
   Cd exposure on LPS-stimulated IL-1 beta and TNF alpha or on
   ConA-stimulated IFN gamma or IL-17 production, but a decrease in IL-6
   production in response to LPS was, again, noted. A preliminary study
   with a lower Cd dose (0.5 mg/kg) revealed some of the same outcomes
   noted here (mononuclear cell infiltration into lungs, increases in PBMC
   IL-1 beta mRNA levels), but differential (increased IL-17 mRNA levels)
   or newly detected outcomes (increased levels of IL-1 alpha mRNA) as
   well. The described effects of the single in vivo exposure to Cd on PBMC
   might contribute to a better overall understanding of the
   immunomodulatory potential of this environmental contaminant.",
journal = "Journal Of Immunotoxicology",
title = "Cadmium administration affects circulatory mononuclear cells in rats",
number = "2",
volume = "12",
doi = "10.3109/1547691X.2014.904955",
pages = "115-123"
}

Djokic, J., Popov Aleksandrov, A., Ninkov, M., Mirkov, I., Zolotarevski, L., Kataranovski, D. S.,& Kataranovski, M.. (2015). Cadmium administration affects circulatory mononuclear cells in rats. in Journal Of Immunotoxicology, 12(2), 115-123.
https://doi.org/10.3109/1547691X.2014.904955

Djokic J, Popov Aleksandrov A, Ninkov M, Mirkov I, Zolotarevski L, Kataranovski DS, Kataranovski M. Cadmium administration affects circulatory mononuclear cells in rats. in Journal Of Immunotoxicology. 2015;12(2):115-123.
doi:10.3109/1547691X.2014.904955 .

Djokic, Jelena, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Mirkov, Ivana, Zolotarevski, Lidija, Kataranovski, Dragan S., Kataranovski, Milena, "Cadmium administration affects circulatory mononuclear cells in rats" in Journal Of Immunotoxicology, 12, no. 2 (2015):115-123,
https://doi.org/10.3109/1547691X.2014.904955 . .

TY  - JOUR
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Petrovic, Anja
AU  - Grigorov, Ilijana
AU  - Zolotarevski, Lidija
AU  - Tolinacki, Maja
AU  - Kataranovski, Dragan S.
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2364
AB  - Gastrointestinal tract is one of the main targets of cadmium (Cd), an
   important food and drinking water contaminant. In the present study, the
   effect of subchronic (30 days) oral (in water) intake of 5ppm and 50ppm
   of cadmium on immune responses in the gut was examined in rats. Cadmium
   consumption resulted in reduction of bacteria corresponding to
   Lactobacillus strain, tissue damage and intestinal inflammation
   {[}increases in high mobility group box 1 (HMGB1 molecules), superoxide
   dismutase (SOD) and catalase (CAT) activity and proinflammatory cytokine
   (TNF, IL-1 beta, IFN-gamma, IL-17) content]. Draining (mesenteric) lymph
   node (MLN) stress response was observed {[}elevation of MLN glutathione
   (GSH) and metallothionein (MT) mRNA levels] and stimulation of both
   adaptive {[}cellularity, proliferation, proinflammatory (IFN-gamma and
   IL-17) MLN cell cytokine responses] as well as innate immune activity
   (increases in numbers of NK and CD68(+) cells, oxidative activities,
   IL-1 beta). In contrast to proinflammatory milieu in MLN, decreased or
   unchanged antiinflammatory IL-10 response was observed. Stimulation of
   immune activities of MLN cells have, most probably, resulted from
   sensing of cadmium-induced tissue injury, but also from bacterial
   antigens that breached compromised intestinal barrier. These effects of
   cadmium should be taken into account when assessing dietary cadmium as
   health risk factor. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
T2  - Toxicology Letters
T1  - Toxicity of oral cadmium intake: Impact on gut immunity
IS  - 2
VL  - 237
DO  - 10.1016/j.toxlet.2015.06.002
SP  - 89
EP  - 99
ER  - 

@article{
author = "Ninkov, Marina and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Mirkov, Ivana and Tucović, Dina and Petrovic, Anja and Grigorov, Ilijana and Zolotarevski, Lidija and Tolinacki, Maja and Kataranovski, Dragan S. and Brceski, Ilija and Kataranovski, Milena",
year = "2015",
abstract = "Gastrointestinal tract is one of the main targets of cadmium (Cd), an
   important food and drinking water contaminant. In the present study, the
   effect of subchronic (30 days) oral (in water) intake of 5ppm and 50ppm
   of cadmium on immune responses in the gut was examined in rats. Cadmium
   consumption resulted in reduction of bacteria corresponding to
   Lactobacillus strain, tissue damage and intestinal inflammation
   {[}increases in high mobility group box 1 (HMGB1 molecules), superoxide
   dismutase (SOD) and catalase (CAT) activity and proinflammatory cytokine
   (TNF, IL-1 beta, IFN-gamma, IL-17) content]. Draining (mesenteric) lymph
   node (MLN) stress response was observed {[}elevation of MLN glutathione
   (GSH) and metallothionein (MT) mRNA levels] and stimulation of both
   adaptive {[}cellularity, proliferation, proinflammatory (IFN-gamma and
   IL-17) MLN cell cytokine responses] as well as innate immune activity
   (increases in numbers of NK and CD68(+) cells, oxidative activities,
   IL-1 beta). In contrast to proinflammatory milieu in MLN, decreased or
   unchanged antiinflammatory IL-10 response was observed. Stimulation of
   immune activities of MLN cells have, most probably, resulted from
   sensing of cadmium-induced tissue injury, but also from bacterial
   antigens that breached compromised intestinal barrier. These effects of
   cadmium should be taken into account when assessing dietary cadmium as
   health risk factor. (C) 2015 Elsevier Ireland Ltd. All rights reserved.",
journal = "Toxicology Letters",
title = "Toxicity of oral cadmium intake: Impact on gut immunity",
number = "2",
volume = "237",
doi = "10.1016/j.toxlet.2015.06.002",
pages = "89-99"
}

Ninkov, M., Popov Aleksandrov, A., Demenesku, J., Mirkov, I., Tucović, D., Petrovic, A., Grigorov, I., Zolotarevski, L., Tolinacki, M., Kataranovski, D. S., Brceski, I.,& Kataranovski, M.. (2015). Toxicity of oral cadmium intake: Impact on gut immunity. in Toxicology Letters, 237(2), 89-99.
https://doi.org/10.1016/j.toxlet.2015.06.002

Ninkov M, Popov Aleksandrov A, Demenesku J, Mirkov I, Tucović D, Petrovic A, Grigorov I, Zolotarevski L, Tolinacki M, Kataranovski DS, Brceski I, Kataranovski M. Toxicity of oral cadmium intake: Impact on gut immunity. in Toxicology Letters. 2015;237(2):89-99.
doi:10.1016/j.toxlet.2015.06.002 .

Ninkov, Marina, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Mirkov, Ivana, Tucović, Dina, Petrovic, Anja, Grigorov, Ilijana, Zolotarevski, Lidija, Tolinacki, Maja, Kataranovski, Dragan S., Brceski, Ilija, Kataranovski, Milena, "Toxicity of oral cadmium intake: Impact on gut immunity" in Toxicology Letters, 237, no. 2 (2015):89-99,
https://doi.org/10.1016/j.toxlet.2015.06.002 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Demenesku, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Glamočlija, Jasmina
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1912
AB  - Although the relevance of genetically-based variations in susceptibility
   to pulmonary aspergillosis was shown in immunocompromised mice and is
   indicated in humans, there is virtually no information concerning
   variations in antifungal immune responses in resistant individuals. We
   have shown recently the relevance of proinflammatory cytokine
   (interferon-gamma/IFN-gamma and interleukin-17/IL-17) responses in
   resistance to sublethal Aspergillus fumigatus infection of
   non-suppressed Dark Agouti (DA) rats (strain known of a substantial
   immune reactivity to noxious insults). In this study, anti-fungal immune
   activities of leukocytes recovered from lungs by enzyme digestion
   (phagocytosis, oxidative activity, hyphal killing, CD11b expression, as
   well as production of IFN-gamma, IL-17 and Th2/anti-inflammatory
   cytokines interleukin-4/IL-4 and interleukin-10/IL-10) were investigated
   in less reactive Albino Oxford (AO) and compared to DA rats. Elimination
   of fungus from lungs of AO rats was associated with lower degree of
   leukocyte infiltration and of the majority of their basic effector
   activities in comparison to DA rats. Lower production of IFN-gamma by
   pulmonary leukocytes was observed early (day 1) post infection (p.i.) in
   AO compared to DA rats, but without changes in IL-4. Both strains
   responded to infection by an increase of IL-17 and IL-10, but production
   of cytokines was higher (from days 7 p.i. and 3 p.i. for IL-17 and
   IL-10, respectively) in AO compared to DA rats. The levels and pattern
   of IFN-gamma and IL-4 responses by draining lymph node (dLN) cells were
   similar in both strains and basically corresponded to those of lung
   leukocytes. In contrast, similar levels of draining lymph node cell
   production of IL-17 and IL-10 were observed in both strains with lack of
   changes in mRNA, what suggests additional stimulation of these cytokines
   in lungs of AO rats. The knowledge of strain differences in the
   immune-based strategies in response of immunocompetent hosts to A.
   fumigatus might contribute to our understanding of variations in
   underlying mechanisms that enable of resistance to this fungus. (C) 2015
   Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Strain differences in the immune mechanisms of resistance of
 immunocompetent rats to pulmonary aspergillosis
IS  - 9
VL  - 220
DO  - 10.1016/j.imbio.2015.05.007
SP  - 1075
EP  - 1084
ER  - 

@article{
author = "Mirkov, Ivana and Demenesku, Jelena and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Glamočlija, Jasmina and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2015",
abstract = "Although the relevance of genetically-based variations in susceptibility
   to pulmonary aspergillosis was shown in immunocompromised mice and is
   indicated in humans, there is virtually no information concerning
   variations in antifungal immune responses in resistant individuals. We
   have shown recently the relevance of proinflammatory cytokine
   (interferon-gamma/IFN-gamma and interleukin-17/IL-17) responses in
   resistance to sublethal Aspergillus fumigatus infection of
   non-suppressed Dark Agouti (DA) rats (strain known of a substantial
   immune reactivity to noxious insults). In this study, anti-fungal immune
   activities of leukocytes recovered from lungs by enzyme digestion
   (phagocytosis, oxidative activity, hyphal killing, CD11b expression, as
   well as production of IFN-gamma, IL-17 and Th2/anti-inflammatory
   cytokines interleukin-4/IL-4 and interleukin-10/IL-10) were investigated
   in less reactive Albino Oxford (AO) and compared to DA rats. Elimination
   of fungus from lungs of AO rats was associated with lower degree of
   leukocyte infiltration and of the majority of their basic effector
   activities in comparison to DA rats. Lower production of IFN-gamma by
   pulmonary leukocytes was observed early (day 1) post infection (p.i.) in
   AO compared to DA rats, but without changes in IL-4. Both strains
   responded to infection by an increase of IL-17 and IL-10, but production
   of cytokines was higher (from days 7 p.i. and 3 p.i. for IL-17 and
   IL-10, respectively) in AO compared to DA rats. The levels and pattern
   of IFN-gamma and IL-4 responses by draining lymph node (dLN) cells were
   similar in both strains and basically corresponded to those of lung
   leukocytes. In contrast, similar levels of draining lymph node cell
   production of IL-17 and IL-10 were observed in both strains with lack of
   changes in mRNA, what suggests additional stimulation of these cytokines
   in lungs of AO rats. The knowledge of strain differences in the
   immune-based strategies in response of immunocompetent hosts to A.
   fumigatus might contribute to our understanding of variations in
   underlying mechanisms that enable of resistance to this fungus. (C) 2015
   Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Strain differences in the immune mechanisms of resistance of
 immunocompetent rats to pulmonary aspergillosis",
number = "9",
volume = "220",
doi = "10.1016/j.imbio.2015.05.007",
pages = "1075-1084"
}

Mirkov, I., Demenesku, J., Popov Aleksandrov, A., Ninkov, M., Glamočlija, J., Kataranovski, D. S.,& Kataranovski, M.. (2015). Strain differences in the immune mechanisms of resistance of
 immunocompetent rats to pulmonary aspergillosis. in Immunobiology, 220(9), 1075-1084.
https://doi.org/10.1016/j.imbio.2015.05.007

Mirkov I, Demenesku J, Popov Aleksandrov A, Ninkov M, Glamočlija J, Kataranovski DS, Kataranovski M. Strain differences in the immune mechanisms of resistance of
 immunocompetent rats to pulmonary aspergillosis. in Immunobiology. 2015;220(9):1075-1084.
doi:10.1016/j.imbio.2015.05.007 .

Mirkov, Ivana, Demenesku, Jelena, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Glamočlija, Jasmina, Kataranovski, Dragan S., Kataranovski, Milena, "Strain differences in the immune mechanisms of resistance of
 immunocompetent rats to pulmonary aspergillosis" in Immunobiology, 220, no. 9 (2015):1075-1084,
https://doi.org/10.1016/j.imbio.2015.05.007 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2092
AB  - Genetic factors are among the most important determinants of
   susceptibility to induction of allergic contact dermatitis. A limited
   number of studies of experimental contact hypersensitivity (CHS) in
   animals has shown differences in the severity of CHS; however, the
   underlying mechanisms are unknown. In this study comparative analysis of
   CHS to low and high dinitrochlorobenzene (DNCB) doses regimen of
   sensitization/challenge in inbred Dark Agouti (DA) and Albino Oxford
   (AO) rats was examined. Basic aspects of draining lymph node (dLN)
   activity (cellularity, proliferation), proinflammatory (IFN-gamma,
   IL-17) and anti-inflammatory (IL-10) cytokine gene expression and
   production, as well as IL-12 and IL-23 subunits mRNA expression, were
   examined in challenge and sensitization phase of CHS reaction. Lower
   (compared to DA) intensity of CHS in AO rats was associated with lack of
   (or negligible) dLN responses in challenge phase (ex vivo, hapten- or
   IL-2-stimulated cell proliferation and proinflammatory cytokine mRNA and
   production levels) but with lack of changes in IL-10 response. Less
   pronounced dLN activity of sensitized animals of this strain was
   observed as well. Higher proliferative activity and more pronounced
   proinflammatory cytokine response during challenge and sensitization
   phase suggest these activities as underlying mechanisms of higher
   susceptibility of DA rats to CHS response to DNCB. (C) 2014 Elsevier
   Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats
VL  - 75
DO  - 10.1016/j.fct.2014.11.010
SP  - 94
EP  - 103
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Demenesku, Jelena and Ninkov, Marina and Zolotarevski, Lidija and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2015",
abstract = "Genetic factors are among the most important determinants of
   susceptibility to induction of allergic contact dermatitis. A limited
   number of studies of experimental contact hypersensitivity (CHS) in
   animals has shown differences in the severity of CHS; however, the
   underlying mechanisms are unknown. In this study comparative analysis of
   CHS to low and high dinitrochlorobenzene (DNCB) doses regimen of
   sensitization/challenge in inbred Dark Agouti (DA) and Albino Oxford
   (AO) rats was examined. Basic aspects of draining lymph node (dLN)
   activity (cellularity, proliferation), proinflammatory (IFN-gamma,
   IL-17) and anti-inflammatory (IL-10) cytokine gene expression and
   production, as well as IL-12 and IL-23 subunits mRNA expression, were
   examined in challenge and sensitization phase of CHS reaction. Lower
   (compared to DA) intensity of CHS in AO rats was associated with lack of
   (or negligible) dLN responses in challenge phase (ex vivo, hapten- or
   IL-2-stimulated cell proliferation and proinflammatory cytokine mRNA and
   production levels) but with lack of changes in IL-10 response. Less
   pronounced dLN activity of sensitized animals of this strain was
   observed as well. Higher proliferative activity and more pronounced
   proinflammatory cytokine response during challenge and sensitization
   phase suggest these activities as underlying mechanisms of higher
   susceptibility of DA rats to CHS response to DNCB. (C) 2014 Elsevier
   Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats",
volume = "75",
doi = "10.1016/j.fct.2014.11.010",
pages = "94-103"
}

Popov Aleksandrov, A., Mirkov, I., Demenesku, J., Ninkov, M., Zolotarevski, L., Kataranovski, D. S.,& Kataranovski, M.. (2015). Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats. in Food and Chemical Toxicology, 75, 94-103.
https://doi.org/10.1016/j.fct.2014.11.010

Popov Aleksandrov A, Mirkov I, Demenesku J, Ninkov M, Zolotarevski L, Kataranovski DS, Kataranovski M. Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats. in Food and Chemical Toxicology. 2015;75:94-103.
doi:10.1016/j.fct.2014.11.010 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Demenesku, Jelena, Ninkov, Marina, Zolotarevski, Lidija, Kataranovski, Dragan S., Kataranovski, Milena, "Strain differences in contact hypersensitivity reaction to
 dinitrochlorobenzene (DNCB) in rats" in Food and Chemical Toxicology, 75 (2015):94-103,
https://doi.org/10.1016/j.fct.2014.11.010 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Tusup, Marina
AU  - Mirkov, Ivana
AU  - Djokic, Jelena
AU  - Ninkov, Marina
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2045
AB  - Context: Dermal toxicity of coumarin anticoagulant rodenticides, such as
   warfarin, represents potential risk for workers handling these agents
   and for individuals applying easily available rodenticides in their
   households as well.
   Objective: In this study, proinflammatory effects of repeated
   epicutaneous administration of warfarin in rats were explored by
   examining inflammatory cytokine skin responses.
   Materials and methods: Ex vivo production of IL-1 beta, IL-6, TNF-alpha
   and IL-17 by skin explants and by epidermal cells isolated by enzyme
   (dispase/trypsin) digestion from skin repeatedly (once a day, three
   consecutive days) exposed to 10 mu g of warfarin was measured 24 h and
   72 h following the last warfarin application by ELISAs for respective
   rat cytokines.
   Results: Warfarin treatment resulted in histological changes, but skin
   or epidermal cell viability were not compromised, judging by MTT
   reduction assay. Both skin and epidermal cells responded to
   administration of this agent by production of all examined inflammatory
   cytokines (skin explants by TNF-alpha and IL-17; epidermal cells by IL-1
   beta and TNF-alpha) except IL-6. Discussion: Along with
   histomorphological changes, cytokines indicate functional consequences
   in treated skin. IL-1 beta production, that precede production of
   TNF-alpha, might be responsible for production of the latter cytokine.
   Sustained production of IL-1 beta suggests persistence of epidermal cell
   stimulation or existence of some amplification mechanisms. Requirements
   for T cells seem to exist concerning epidermal cell IL-17 production.
   Conclusion: Presented data provide additional new information concerning
   proinflammatory effects of warfarin.
T2  - Cutaneous and Ocular Toxicology
T1  - Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats
IS  - 2
VL  - 34
DO  - 10.3109/15569527.2014.928307
SP  - 149
EP  - 155
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Tusup, Marina and Mirkov, Ivana and Djokic, Jelena and Ninkov, Marina and Zolotarevski, Lidija and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2015",
abstract = "Context: Dermal toxicity of coumarin anticoagulant rodenticides, such as
   warfarin, represents potential risk for workers handling these agents
   and for individuals applying easily available rodenticides in their
   households as well.
   Objective: In this study, proinflammatory effects of repeated
   epicutaneous administration of warfarin in rats were explored by
   examining inflammatory cytokine skin responses.
   Materials and methods: Ex vivo production of IL-1 beta, IL-6, TNF-alpha
   and IL-17 by skin explants and by epidermal cells isolated by enzyme
   (dispase/trypsin) digestion from skin repeatedly (once a day, three
   consecutive days) exposed to 10 mu g of warfarin was measured 24 h and
   72 h following the last warfarin application by ELISAs for respective
   rat cytokines.
   Results: Warfarin treatment resulted in histological changes, but skin
   or epidermal cell viability were not compromised, judging by MTT
   reduction assay. Both skin and epidermal cells responded to
   administration of this agent by production of all examined inflammatory
   cytokines (skin explants by TNF-alpha and IL-17; epidermal cells by IL-1
   beta and TNF-alpha) except IL-6. Discussion: Along with
   histomorphological changes, cytokines indicate functional consequences
   in treated skin. IL-1 beta production, that precede production of
   TNF-alpha, might be responsible for production of the latter cytokine.
   Sustained production of IL-1 beta suggests persistence of epidermal cell
   stimulation or existence of some amplification mechanisms. Requirements
   for T cells seem to exist concerning epidermal cell IL-17 production.
   Conclusion: Presented data provide additional new information concerning
   proinflammatory effects of warfarin.",
journal = "Cutaneous and Ocular Toxicology",
title = "Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats",
number = "2",
volume = "34",
doi = "10.3109/15569527.2014.928307",
pages = "149-155"
}

Popov Aleksandrov, A., Tusup, M., Mirkov, I., Djokic, J., Ninkov, M., Zolotarevski, L., Kataranovski, D. S.,& Kataranovski, M.. (2015). Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats. in Cutaneous and Ocular Toxicology, 34(2), 149-155.
https://doi.org/10.3109/15569527.2014.928307

Popov Aleksandrov A, Tusup M, Mirkov I, Djokic J, Ninkov M, Zolotarevski L, Kataranovski DS, Kataranovski M. Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats. in Cutaneous and Ocular Toxicology. 2015;34(2):149-155.
doi:10.3109/15569527.2014.928307 .

Popov Aleksandrov, Aleksandra, Tusup, Marina, Mirkov, Ivana, Djokic, Jelena, Ninkov, Marina, Zolotarevski, Lidija, Kataranovski, Dragan S., Kataranovski, Milena, "Proinflammatory cytokine responses in skin and epidermal cells following
 epicutaneous administration of anticoagulant rodenticide warfarin in
 rats" in Cutaneous and Ocular Toxicology, 34, no. 2 (2015):149-155,
https://doi.org/10.3109/15569527.2014.928307 . .

TY  - JOUR
AU  - Djokic, J.
AU  - Ninkov, Marina
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Zolotarevski, L.
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2298
AB  - Infiltration of circulatory inflammatory cells is a common
   histopathological finding in target organs following cadmium
   administration, but there is paucity of data concerning their activity.
   In this study, the effects of sublethal (1 mg/kg) cadmium on peripheral
   blood polymorphonuclear (PMN) cells were examined 48 h following
   administration in rats, when tissue (liver and lung) infiltration of
   these cells was observed. Cadmium administration resulted in systemic
   inflammatory cytokine and acute phase response with an increase in
   circulatory neutrophil numbers and cells that express CD11b molecules.
   Rise in basic aspects of oxidative activity including intracellular
   myeloperoxidase (MPO), reactive oxygen (nitroblue tetrazolium/NBT
   cytochemical assay) and nitrogen (Griess assay) species production was
   observed in PMNs from cadmium-administered rats. A decrease in levels of
   mRNA for IL-1 beta, TNF-alpha and IL-6 was noted, but production of
   these cytokines was affected differentially. Described effects of
   cadmium on PMNs add further to the understanding of inflammatory
   potential of this environmental contaminant. (C) 2013 Elsevier B.V. All
   rights reserved.
T2  - Environmental Toxicology and Pharmacology
T1  - Differential effects of cadmium administration on peripheral blood
 granulocytes in rats
IS  - 1
VL  - 37
DO  - 10.1016/j.etap.2013.11.026
SP  - 210
EP  - 219
ER  - 

@article{
author = "Djokic, J. and Ninkov, Marina and Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Zolotarevski, L. and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2014",
abstract = "Infiltration of circulatory inflammatory cells is a common
   histopathological finding in target organs following cadmium
   administration, but there is paucity of data concerning their activity.
   In this study, the effects of sublethal (1 mg/kg) cadmium on peripheral
   blood polymorphonuclear (PMN) cells were examined 48 h following
   administration in rats, when tissue (liver and lung) infiltration of
   these cells was observed. Cadmium administration resulted in systemic
   inflammatory cytokine and acute phase response with an increase in
   circulatory neutrophil numbers and cells that express CD11b molecules.
   Rise in basic aspects of oxidative activity including intracellular
   myeloperoxidase (MPO), reactive oxygen (nitroblue tetrazolium/NBT
   cytochemical assay) and nitrogen (Griess assay) species production was
   observed in PMNs from cadmium-administered rats. A decrease in levels of
   mRNA for IL-1 beta, TNF-alpha and IL-6 was noted, but production of
   these cytokines was affected differentially. Described effects of
   cadmium on PMNs add further to the understanding of inflammatory
   potential of this environmental contaminant. (C) 2013 Elsevier B.V. All
   rights reserved.",
journal = "Environmental Toxicology and Pharmacology",
title = "Differential effects of cadmium administration on peripheral blood
 granulocytes in rats",
number = "1",
volume = "37",
doi = "10.1016/j.etap.2013.11.026",
pages = "210-219"
}

Djokic, J., Ninkov, M., Mirkov, I., Popov Aleksandrov, A., Zolotarevski, L., Kataranovski, D. S.,& Kataranovski, M.. (2014). Differential effects of cadmium administration on peripheral blood
 granulocytes in rats. in Environmental Toxicology and Pharmacology, 37(1), 210-219.
https://doi.org/10.1016/j.etap.2013.11.026

Djokic J, Ninkov M, Mirkov I, Popov Aleksandrov A, Zolotarevski L, Kataranovski DS, Kataranovski M. Differential effects of cadmium administration on peripheral blood
 granulocytes in rats. in Environmental Toxicology and Pharmacology. 2014;37(1):210-219.
doi:10.1016/j.etap.2013.11.026 .

Djokic, J., Ninkov, Marina, Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Zolotarevski, L., Kataranovski, Dragan S., Kataranovski, Milena, "Differential effects of cadmium administration on peripheral blood
 granulocytes in rats" in Environmental Toxicology and Pharmacology, 37, no. 1 (2014):210-219,
https://doi.org/10.1016/j.etap.2013.11.026 . .

TY  - JOUR
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Zolotarevski, Lidija
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2103
AB  - Conflicting data (both suppression and augmentation as well as lack of
   the effect) exist in respect to cadmium (Cd) and splenic T cell-based
   immune cell activity. Spleen is also the site of innate immune responses
   but impact of Cd on this type of immunity has been less explored. In the
   present study the effects of acute Cd administration on basic aspects of
   both T cell-based and innate immune spleen cell activity were examined
   in rats. Intraperitoneal injection of 1 mg of Cd/kg resulted in decrease
   in concanavalin A (ConA) induced proliferation which seems to be more
   related to altered spleen cells responsiveness to IL-2 than to
   apoptosis. Differential effects on proinflammatory T cell derived
   cytokines were observed (decreases of IFN-gamma gene expression and
   ConA-stimulated production, but increases in IL-17 mRNA levels with no
   effect on concentrations of protein product). Reduction of IFN-gamma
   production seemed not to rely on IL-4 and IL-10, but at least partly on
   nitric oxide (NO). Increased activity relevant for innate immunity
   (granulocyte and CD11b(+) cell accumulation in the spleen, inducible
   nitric oxide synthase/iNOS expression and NO production by spleen cells)
   was observed, but there was a decrease in respiratory burst
   (dihydrorhodamine/DHR oxidation and nitroblue tetrazolium/NBT
   reduction). Increases of TNF-alpha and IL-1 beta gene expression and
   IL-1 beta protein product were noted as well. Administration of 0.5 mg
   Cd/kg resulted in less pronounced (ConA-induced proliferation) or lack
   of the effect (IFN-gamma production) on spleen T cell activities and on
   innate activities (granulocyte accumulation, NO production) as well.
   However, increases of spleen cell respiratory burst activity and IL-1
   beta production were observed. Effects of lower cadmium doses (5 ppm and
   50 ppm) on several aspects of spleen cell immune activity were observed
   in intermediate period of exposure (30 days, oral intake) as well.
   Differential effects of Cd on immune activities of spleen cells might
   contribute to our understanding of the complexity of immunomodulatory
   effects of this metal. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.
T2  - Toxicology
T1  - Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen
VL  - 326
DO  - 10.1016/j.tox.2014.10.012
SP  - 96
EP  - 108
ER  - 

@article{
author = "Demenesku, Jelena and Mirkov, Ivana and Ninkov, Marina and Popov Aleksandrov, Aleksandra and Zolotarevski, Lidija and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2014",
abstract = "Conflicting data (both suppression and augmentation as well as lack of
   the effect) exist in respect to cadmium (Cd) and splenic T cell-based
   immune cell activity. Spleen is also the site of innate immune responses
   but impact of Cd on this type of immunity has been less explored. In the
   present study the effects of acute Cd administration on basic aspects of
   both T cell-based and innate immune spleen cell activity were examined
   in rats. Intraperitoneal injection of 1 mg of Cd/kg resulted in decrease
   in concanavalin A (ConA) induced proliferation which seems to be more
   related to altered spleen cells responsiveness to IL-2 than to
   apoptosis. Differential effects on proinflammatory T cell derived
   cytokines were observed (decreases of IFN-gamma gene expression and
   ConA-stimulated production, but increases in IL-17 mRNA levels with no
   effect on concentrations of protein product). Reduction of IFN-gamma
   production seemed not to rely on IL-4 and IL-10, but at least partly on
   nitric oxide (NO). Increased activity relevant for innate immunity
   (granulocyte and CD11b(+) cell accumulation in the spleen, inducible
   nitric oxide synthase/iNOS expression and NO production by spleen cells)
   was observed, but there was a decrease in respiratory burst
   (dihydrorhodamine/DHR oxidation and nitroblue tetrazolium/NBT
   reduction). Increases of TNF-alpha and IL-1 beta gene expression and
   IL-1 beta protein product were noted as well. Administration of 0.5 mg
   Cd/kg resulted in less pronounced (ConA-induced proliferation) or lack
   of the effect (IFN-gamma production) on spleen T cell activities and on
   innate activities (granulocyte accumulation, NO production) as well.
   However, increases of spleen cell respiratory burst activity and IL-1
   beta production were observed. Effects of lower cadmium doses (5 ppm and
   50 ppm) on several aspects of spleen cell immune activity were observed
   in intermediate period of exposure (30 days, oral intake) as well.
   Differential effects of Cd on immune activities of spleen cells might
   contribute to our understanding of the complexity of immunomodulatory
   effects of this metal. (C) 2014 Elsevier Ireland Ltd. All rights
   reserved.",
journal = "Toxicology",
title = "Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen",
volume = "326",
doi = "10.1016/j.tox.2014.10.012",
pages = "96-108"
}

Demenesku, J., Mirkov, I., Ninkov, M., Popov Aleksandrov, A., Zolotarevski, L., Kataranovski, D. S.,& Kataranovski, M.. (2014). Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen. in Toxicology, 326, 96-108.
https://doi.org/10.1016/j.tox.2014.10.012

Demenesku J, Mirkov I, Ninkov M, Popov Aleksandrov A, Zolotarevski L, Kataranovski DS, Kataranovski M. Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen. in Toxicology. 2014;326:96-108.
doi:10.1016/j.tox.2014.10.012 .

Demenesku, Jelena, Mirkov, Ivana, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Zolotarevski, Lidija, Kataranovski, Dragan S., Kataranovski, Milena, "Acute cadmium administration to rats exerts both immunosuppressive and
 proinflammatory effects in spleen" in Toxicology, 326 (2014):96-108,
https://doi.org/10.1016/j.tox.2014.10.012 . .

TY  - JOUR
AU  - Jokic, G.
AU  - Vuksa, Marina
AU  - Dedovic, Suzana
AU  - Stojnic, B.
AU  - Kataranovski, Dragan S.
AU  - Kljajic, P.
AU  - Jacevic, Vesna
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2257
AB  - We examined the acceptance and palatability of baits containing
   different contents of sodium selenite as a rodenticide, in Swiss mice
   under laboratory conditions. In a no-choice and choice feeding test, the
   animals were exposed to baits containing 0.1, 0.05, 0.025 and 0.0125\%
   of sodium selenite. The total bait consumption by Swiss mice in the
   no-choice feeding test was highly negatively correlated, while total
   sodium selenite intake was medium-positively correlated to the sodium
   selenite content in the bait. In the same test, daily intakes
   significantly depended on the content of sodium selenite in the bait,
   while the exposure and associated interactions of contents of sodium
   selenite and exposure had no statistically significant impact. Baits
   with sodium selenite contents of 0.05 and 0.1\% had the most lethal
   effects. The negative impact of the sodium selenite content on bait
   acceptance and palatability was confirmed in choice feeding tests. Baits
   containing 0.05 and 0.1\% of sodium selenite displayed the biological
   potential to be used as a rodenticide. It is necessary to improve its
   insufficient acceptability and palatability by adding adequate additives
   to the bait. The results of this study should be verified in experiments
   with wild rodents.
T2  - Archives of Biological Sciences
T1  - Rodenticide efficacy of sodium selenite baits in laboratory conditions
IS  - 3
VL  - 66
DO  - 10.2298/ABS1403083J
SP  - 1083
EP  - 1089
ER  - 

@article{
author = "Jokic, G. and Vuksa, Marina and Dedovic, Suzana and Stojnic, B. and Kataranovski, Dragan S. and Kljajic, P. and Jacevic, Vesna",
year = "2014",
abstract = "We examined the acceptance and palatability of baits containing
   different contents of sodium selenite as a rodenticide, in Swiss mice
   under laboratory conditions. In a no-choice and choice feeding test, the
   animals were exposed to baits containing 0.1, 0.05, 0.025 and 0.0125\%
   of sodium selenite. The total bait consumption by Swiss mice in the
   no-choice feeding test was highly negatively correlated, while total
   sodium selenite intake was medium-positively correlated to the sodium
   selenite content in the bait. In the same test, daily intakes
   significantly depended on the content of sodium selenite in the bait,
   while the exposure and associated interactions of contents of sodium
   selenite and exposure had no statistically significant impact. Baits
   with sodium selenite contents of 0.05 and 0.1\% had the most lethal
   effects. The negative impact of the sodium selenite content on bait
   acceptance and palatability was confirmed in choice feeding tests. Baits
   containing 0.05 and 0.1\% of sodium selenite displayed the biological
   potential to be used as a rodenticide. It is necessary to improve its
   insufficient acceptability and palatability by adding adequate additives
   to the bait. The results of this study should be verified in experiments
   with wild rodents.",
journal = "Archives of Biological Sciences",
title = "Rodenticide efficacy of sodium selenite baits in laboratory conditions",
number = "3",
volume = "66",
doi = "10.2298/ABS1403083J",
pages = "1083-1089"
}

Jokic, G., Vuksa, M., Dedovic, S., Stojnic, B., Kataranovski, D. S., Kljajic, P.,& Jacevic, V.. (2014). Rodenticide efficacy of sodium selenite baits in laboratory conditions. in Archives of Biological Sciences, 66(3), 1083-1089.
https://doi.org/10.2298/ABS1403083J

Jokic G, Vuksa M, Dedovic S, Stojnic B, Kataranovski DS, Kljajic P, Jacevic V. Rodenticide efficacy of sodium selenite baits in laboratory conditions. in Archives of Biological Sciences. 2014;66(3):1083-1089.
doi:10.2298/ABS1403083J .

Jokic, G., Vuksa, Marina, Dedovic, Suzana, Stojnic, B., Kataranovski, Dragan S., Kljajic, P., Jacevic, Vesna, "Rodenticide efficacy of sodium selenite baits in laboratory conditions" in Archives of Biological Sciences, 66, no. 3 (2014):1083-1089,
https://doi.org/10.2298/ABS1403083J . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Belij, Sandra
AU  - Subota, Vesna S
AU  - Zolotarevski, Lidija D
AU  - Mirkov, Ivana
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1061
AB  - Warfarin is a Vitamin K (VK) antagonist that affects Vitamin K-dependent (VKD) processes, including blood coagulation, as well as processes unrelated to hemostasis such as bone growth, calcification, and growth of some cell types. In addition, warfarin exerts influence on some non-VKD-related activities, including anti-tumor and immunomodulating activity. With respect to the latter, both immune stimulating and suppressive effects have been noted in different experimental systems. To explore the in vivo immunomodulatory potential of warfarin on one type of activity (i.e., cytokine production) in two different immune cell populations (i.e., mononuclear or polymorphonuclear cells), effects of subchronic oral warfarin intake in rats on pro-inflammatory cytokine (i.e., TNF alpha, IL-6) production by peripheral blood mononuclear and polymorphonuclear cells (granulocytes) was examined. Differential effects of warfarin intake on TNF alpha and IL-6 were noted, depending on the type of peripheral blood leukocytes and on the cytokine examined. Specifically, a lack of effect on TNF alpha and a priming of IL-6 production by mononuclear cells along with a decrease in TNF alpha and a lack of effect on IL-6 in polymorphonuclear cells were seen in warfarin-exposed hosts. The cell-and cytokine-dependent effects from subchronic oral warfarin intake on peripheral blood leukocytes demonstrated in this study could, possibly, differentially affect reactions mediated by these cells. Ultimately, the observed effects in rats might have implications for those humans who are on long-term/prolonged warfarin therapy.
T2  - Journal of Immunotoxicology
T1  - Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats
IS  - 1
VL  - 10
SP  - 375
EP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1061
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Belij, Sandra and Subota, Vesna S and Zolotarevski, Lidija D and Mirkov, Ivana and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2013",
abstract = "Warfarin is a Vitamin K (VK) antagonist that affects Vitamin K-dependent (VKD) processes, including blood coagulation, as well as processes unrelated to hemostasis such as bone growth, calcification, and growth of some cell types. In addition, warfarin exerts influence on some non-VKD-related activities, including anti-tumor and immunomodulating activity. With respect to the latter, both immune stimulating and suppressive effects have been noted in different experimental systems. To explore the in vivo immunomodulatory potential of warfarin on one type of activity (i.e., cytokine production) in two different immune cell populations (i.e., mononuclear or polymorphonuclear cells), effects of subchronic oral warfarin intake in rats on pro-inflammatory cytokine (i.e., TNF alpha, IL-6) production by peripheral blood mononuclear and polymorphonuclear cells (granulocytes) was examined. Differential effects of warfarin intake on TNF alpha and IL-6 were noted, depending on the type of peripheral blood leukocytes and on the cytokine examined. Specifically, a lack of effect on TNF alpha and a priming of IL-6 production by mononuclear cells along with a decrease in TNF alpha and a lack of effect on IL-6 in polymorphonuclear cells were seen in warfarin-exposed hosts. The cell-and cytokine-dependent effects from subchronic oral warfarin intake on peripheral blood leukocytes demonstrated in this study could, possibly, differentially affect reactions mediated by these cells. Ultimately, the observed effects in rats might have implications for those humans who are on long-term/prolonged warfarin therapy.",
journal = "Journal of Immunotoxicology",
title = "Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats",
number = "1",
volume = "10",
pages = "375-24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1061"
}

Popov Aleksandrov, A., Belij, S., Subota, V. S., Zolotarevski, L. D., Mirkov, I., Kataranovski, D. S.,& Kataranovski, M.. (2013). Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats. in Journal of Immunotoxicology, 10(1), 375-24.
https://hdl.handle.net/21.15107/rcub_ibiss_1061

Popov Aleksandrov A, Belij S, Subota VS, Zolotarevski LD, Mirkov I, Kataranovski DS, Kataranovski M. Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats. in Journal of Immunotoxicology. 2013;10(1):375-24.
https://hdl.handle.net/21.15107/rcub_ibiss_1061 .

Popov Aleksandrov, Aleksandra, Belij, Sandra, Subota, Vesna S, Zolotarevski, Lidija D, Mirkov, Ivana, Kataranovski, Dragan S., Kataranovski, Milena, "Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats" in Journal of Immunotoxicology, 10, no. 1 (2013):375-24,
https://hdl.handle.net/21.15107/rcub_ibiss_1061 .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Vasilijić, Sasa R
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/961
AB  - Allergic contact dermatitis (ACD) is a common skin inflammatory disease that develops in hosts sensitized with contact allergens. Elucidation of dose-response relationships represents one of the approaches in studying the type of ACD in humans/animal models, termed as contact hyper-sensitivity reaction (CHS). Such studies have demonstrated that the intensity of sensitization determines the response to elicitation with a contact allergen, but underlying mechanisms are unclear. The aim of this study is to explore the impact of the sensitization on contact hypersensitivity to dinitrochlorobenzene (DNCB) in rats by measuring the incidence and intensity of a challenge response in hosts sensitized with two different doses (i.e. low and high) of this hapten. Assumptions concerning the contribution from the magnitude of sensitization doses were drawn on the basis of effects from the two doses on the measured reaction parameters. Ear swelling and activity of lymph nodes that drain challenged skin (cdLN), including cellularity, proliferation, and effector cytokine IFN gamma and IL-17 production was measured in rats sensitized with 0.4% or 4% DNCB and challenged with a non-irritant (0.13%) dose. Sensitization with 4% DNCB resulted in a greater proportion of rats who responded more intensely (than unsensitized challenged rats) to challenge in terms of ear swelling and increases in cdLN activity (except for IFN gamma). The intensity of cdLN responses was higher in these hosts as well. Among the high-dose-sensitized rats, greater cellularity/proliferation of cells from lymph nodes (sdLN) that drain the high-dose-sensitized skin, as well as higher IL-17 production, was noted compared to what was seen in rats that received low-dose sensitization. In contrast, unchanged spontaneous and even decreased hapten-stimulated IFN gamma production after the high DNCB dose was observed. Based on the data, it seems the impact of magnitude of sensitization dose on CHS might be related to the rise in the proportion of rats that responded to challenge with an increase of dLN activity. Coincidental higher production of IL-17 by dLN cells from the high-dose-sensitized rats and following challenge of these hosts underscored the significance of IL-17 for a CHS to DNCB.
T2  - Journal of Immunotoxicology
T1  - Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats
IS  - 4
VL  - 10
SP  - 165
EP  - 360
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_961
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Vasilijić, Sasa R and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2013",
abstract = "Allergic contact dermatitis (ACD) is a common skin inflammatory disease that develops in hosts sensitized with contact allergens. Elucidation of dose-response relationships represents one of the approaches in studying the type of ACD in humans/animal models, termed as contact hyper-sensitivity reaction (CHS). Such studies have demonstrated that the intensity of sensitization determines the response to elicitation with a contact allergen, but underlying mechanisms are unclear. The aim of this study is to explore the impact of the sensitization on contact hypersensitivity to dinitrochlorobenzene (DNCB) in rats by measuring the incidence and intensity of a challenge response in hosts sensitized with two different doses (i.e. low and high) of this hapten. Assumptions concerning the contribution from the magnitude of sensitization doses were drawn on the basis of effects from the two doses on the measured reaction parameters. Ear swelling and activity of lymph nodes that drain challenged skin (cdLN), including cellularity, proliferation, and effector cytokine IFN gamma and IL-17 production was measured in rats sensitized with 0.4% or 4% DNCB and challenged with a non-irritant (0.13%) dose. Sensitization with 4% DNCB resulted in a greater proportion of rats who responded more intensely (than unsensitized challenged rats) to challenge in terms of ear swelling and increases in cdLN activity (except for IFN gamma). The intensity of cdLN responses was higher in these hosts as well. Among the high-dose-sensitized rats, greater cellularity/proliferation of cells from lymph nodes (sdLN) that drain the high-dose-sensitized skin, as well as higher IL-17 production, was noted compared to what was seen in rats that received low-dose sensitization. In contrast, unchanged spontaneous and even decreased hapten-stimulated IFN gamma production after the high DNCB dose was observed. Based on the data, it seems the impact of magnitude of sensitization dose on CHS might be related to the rise in the proportion of rats that responded to challenge with an increase of dLN activity. Coincidental higher production of IL-17 by dLN cells from the high-dose-sensitized rats and following challenge of these hosts underscored the significance of IL-17 for a CHS to DNCB.",
journal = "Journal of Immunotoxicology",
title = "Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats",
number = "4",
volume = "10",
pages = "165-360",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_961"
}

Popov Aleksandrov, A., Mirkov, I., Vasilijić, S. R., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2013). Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats. in Journal of Immunotoxicology, 10(4), 165-360.
https://hdl.handle.net/21.15107/rcub_ibiss_961

Popov Aleksandrov A, Mirkov I, Vasilijić SR, Zolotarevski LD, Kataranovski DS, Kataranovski M. Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats. in Journal of Immunotoxicology. 2013;10(4):165-360.
https://hdl.handle.net/21.15107/rcub_ibiss_961 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Vasilijić, Sasa R, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Impact of the magnitude of sensitization dose on the incidence and intensity of CHS to dinitrochlorobenzene (DNCB): Insight from ear swelling and challenged-skin draining lymph node response in rats" in Journal of Immunotoxicology, 10, no. 4 (2013):165-360,
https://hdl.handle.net/21.15107/rcub_ibiss_961 .

TY  - JOUR
AU  - El-Muzghi, Amal Atia Mhfuod
AU  - Mirkov, Ivana
AU  - Đokić, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Miljković, Đorđe
AU  - Glamočlija, Jasmina
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/945
AB  - Rat models of pulmonary aspergillosis are used widely in diagnostic studies and in exploring antifungal therapeutic modalities, but there is lack of data concerning antifungal immunity in rats: In this study, cytokine response to pulmonary infection to Aspergillus fumigatus in non-immunosuppressed rats is explored. Temporal display (from the start of infection up to its eradication) of proinflammatory cytokines (IFN-gamma and IL-17) as well as Th2/anti-inflammatory ones (IL-4 and IL-10) was explored by measuring their presence in the environment in which elimination of infection occur (lung homogenates), by production of these mediators by lung cells (recovered by enzyme digestion or by bronchoalveolar lavage) as well as by cells of draining lymph nodes (as sites of generation of cytokine-producing cells). Reduction of infection (1 x 10(7) conidia) was associated with an increase of IFN-gamma and IL-17 content in lung homogenates, but with unchanged IL-4 and IL-10 content. Lung cells produced proinflammatory cytokines with differential dynamics (IFN-gamma earlier than IL-17). Differential pattern of Th2/anti-inflammatory cytokine production by lung cells was observed (unchanged IL-4 and increased IL-10), with the levels of the latter higher than proinflammatory cytokines. Upregulation of IFN-gamma, IL-17 and IL-10 production and gene expression, but downregulation of IL-4, by draining lymph node cells (dLN cells) accounted essentially for the observed ex vivo cytokine response in lungs. Similar pattern of cytokine production by dLN cells following restimulation with A. fumigatus conidia confirmed the specificity of cytokine response to the fungus. Draining lymph node CD4(+) cells seems to be the main source of proinflammatory cytokines, significant contributors to IL-10 production and the target for down regulation of IL-4. The knowledge of immune-based mechanisms of defense against A. fumigatus in rats might be helpful in the future use of rat models of pulmonary aspergillosis particularly those that develop immune-based therapeutic interventions as an adjunct treatment of fungal diseases. (C) 2013 Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats
IS  - 12
VL  - 218
SP  - 69
EP  - 1523
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_945
ER  - 

@article{
author = "El-Muzghi, Amal Atia Mhfuod and Mirkov, Ivana and Đokić, Jelena and Popov Aleksandrov, Aleksandra and Miljković, Đorđe and Glamočlija, Jasmina and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2013",
abstract = "Rat models of pulmonary aspergillosis are used widely in diagnostic studies and in exploring antifungal therapeutic modalities, but there is lack of data concerning antifungal immunity in rats: In this study, cytokine response to pulmonary infection to Aspergillus fumigatus in non-immunosuppressed rats is explored. Temporal display (from the start of infection up to its eradication) of proinflammatory cytokines (IFN-gamma and IL-17) as well as Th2/anti-inflammatory ones (IL-4 and IL-10) was explored by measuring their presence in the environment in which elimination of infection occur (lung homogenates), by production of these mediators by lung cells (recovered by enzyme digestion or by bronchoalveolar lavage) as well as by cells of draining lymph nodes (as sites of generation of cytokine-producing cells). Reduction of infection (1 x 10(7) conidia) was associated with an increase of IFN-gamma and IL-17 content in lung homogenates, but with unchanged IL-4 and IL-10 content. Lung cells produced proinflammatory cytokines with differential dynamics (IFN-gamma earlier than IL-17). Differential pattern of Th2/anti-inflammatory cytokine production by lung cells was observed (unchanged IL-4 and increased IL-10), with the levels of the latter higher than proinflammatory cytokines. Upregulation of IFN-gamma, IL-17 and IL-10 production and gene expression, but downregulation of IL-4, by draining lymph node cells (dLN cells) accounted essentially for the observed ex vivo cytokine response in lungs. Similar pattern of cytokine production by dLN cells following restimulation with A. fumigatus conidia confirmed the specificity of cytokine response to the fungus. Draining lymph node CD4(+) cells seems to be the main source of proinflammatory cytokines, significant contributors to IL-10 production and the target for down regulation of IL-4. The knowledge of immune-based mechanisms of defense against A. fumigatus in rats might be helpful in the future use of rat models of pulmonary aspergillosis particularly those that develop immune-based therapeutic interventions as an adjunct treatment of fungal diseases. (C) 2013 Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats",
number = "12",
volume = "218",
pages = "69-1523",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_945"
}

El-Muzghi, A. A. M., Mirkov, I., Đokić, J., Popov Aleksandrov, A., Miljković, Đ., Glamočlija, J., Kataranovski, D. S.,& Kataranovski, M.. (2013). Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats. in Immunobiology, 218(12), 69-1523.
https://hdl.handle.net/21.15107/rcub_ibiss_945

El-Muzghi AAM, Mirkov I, Đokić J, Popov Aleksandrov A, Miljković Đ, Glamočlija J, Kataranovski DS, Kataranovski M. Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats. in Immunobiology. 2013;218(12):69-1523.
https://hdl.handle.net/21.15107/rcub_ibiss_945 .

El-Muzghi, Amal Atia Mhfuod, Mirkov, Ivana, Đokić, Jelena, Popov Aleksandrov, Aleksandra, Miljković, Đorđe, Glamočlija, Jasmina, Kataranovski, Dragan S., Kataranovski, Milena, "Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats" in Immunobiology, 218, no. 12 (2013):69-1523,
https://hdl.handle.net/21.15107/rcub_ibiss_945 .

TY  - JOUR
AU  - Glamočlija, Jasmina
AU  - Mirkov, Ivana
AU  - Kataranovski, Milena
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Stošić-Grujičić, Stanislava
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1039
AB  - Mirkov I, Glamoclija J, Stosic-Grujicic S, Zolotarevski L, Kataranovski D, Kataranovski M. Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice. APMIS 2013; 121: 211-20. Using a nonlethal systemic Aspergillus fumigatus infection, we have recently shown that similarly efficient elimination of fungus from spleens of prototypic Th1 (C57BL/6) and prototypic Th2 (BALB/c) mice is associated with differential immune responses. In light of these data and given the disseminated character of infection, the aim of the present study is to explore whether there are also strain-dependent differences in antifungal responses in peripheral tissues of infected mice. Although similar efficiency of conidia removal was noted in liver and kidneys of both strains, BALB/c mice seemed more prone to tissue injury. Compared with other nonlymphoid organs, lungs proved immunologically the most responsive in systemic aspergillosis. Lower numbers of neutrophils and macrophages in the lungs of infected BALB/c mice, delayed and lower (compared with C57BL/6 mice) expression of their oxidative activity, along with late IFN- and upregulated IL-4 production by lung cells might be responsible for slower elimination of A. fumigatus from the lungs of this mouse strain. The data obtained imply that lungs should be viewed as mandatory organ in evaluation of immune-mediated antifungal potential of drugs in models of systemic/disseminated infection and that strain differences noted in tissue responses should be taken into account in these settings.
T2  - Apmis
T1  - Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice
IS  - 3
VL  - 121
SP  - 493
EP  - 220
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1039
ER  - 

@article{
author = "Glamočlija, Jasmina and Mirkov, Ivana and Kataranovski, Milena and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Stošić-Grujičić, Stanislava",
year = "2013",
abstract = "Mirkov I, Glamoclija J, Stosic-Grujicic S, Zolotarevski L, Kataranovski D, Kataranovski M. Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice. APMIS 2013; 121: 211-20. Using a nonlethal systemic Aspergillus fumigatus infection, we have recently shown that similarly efficient elimination of fungus from spleens of prototypic Th1 (C57BL/6) and prototypic Th2 (BALB/c) mice is associated with differential immune responses. In light of these data and given the disseminated character of infection, the aim of the present study is to explore whether there are also strain-dependent differences in antifungal responses in peripheral tissues of infected mice. Although similar efficiency of conidia removal was noted in liver and kidneys of both strains, BALB/c mice seemed more prone to tissue injury. Compared with other nonlymphoid organs, lungs proved immunologically the most responsive in systemic aspergillosis. Lower numbers of neutrophils and macrophages in the lungs of infected BALB/c mice, delayed and lower (compared with C57BL/6 mice) expression of their oxidative activity, along with late IFN- and upregulated IL-4 production by lung cells might be responsible for slower elimination of A. fumigatus from the lungs of this mouse strain. The data obtained imply that lungs should be viewed as mandatory organ in evaluation of immune-mediated antifungal potential of drugs in models of systemic/disseminated infection and that strain differences noted in tissue responses should be taken into account in these settings.",
journal = "Apmis",
title = "Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice",
number = "3",
volume = "121",
pages = "493-220",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1039"
}

Glamočlija, J., Mirkov, I., Kataranovski, M., Zolotarevski, L. D., Kataranovski, D. S.,& Stošić-Grujičić, S.. (2013). Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice. in Apmis, 121(3), 493-220.
https://hdl.handle.net/21.15107/rcub_ibiss_1039

Glamočlija J, Mirkov I, Kataranovski M, Zolotarevski LD, Kataranovski DS, Stošić-Grujičić S. Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice. in Apmis. 2013;121(3):493-220.
https://hdl.handle.net/21.15107/rcub_ibiss_1039 .

Glamočlija, Jasmina, Mirkov, Ivana, Kataranovski, Milena, Zolotarevski, Lidija D, Kataranovski, Dragan S., Stošić-Grujičić, Stanislava, "Differential strain-related tissue immune response to sublethal systemic Aspergillus fumigatus infection in mice" in Apmis, 121, no. 3 (2013):493-220,
https://hdl.handle.net/21.15107/rcub_ibiss_1039 .

TY  - CONF
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Miljković, Đorđe
AU  - Belij, Sandra
AU  - Đokić, Jelena
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1113
C3  - Immunology
T1  - Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats
IS  - null
VL  - 137
SP  - 349
EP  - 442
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1113
ER  - 

@conference{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Miljković, Đorđe and Belij, Sandra and Đokić, Jelena and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
journal = "Immunology",
title = "Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats",
number = "null",
volume = "137",
pages = "349-442",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1113"
}

Popov Aleksandrov, A., Mirkov, I., Miljković, Đ., Belij, S., Đokić, J., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2012). Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats. in Immunology, 137(null), 349-442.
https://hdl.handle.net/21.15107/rcub_ibiss_1113

Popov Aleksandrov A, Mirkov I, Miljković Đ, Belij S, Đokić J, Zolotarevski LD, Kataranovski DS, Kataranovski M. Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats. in Immunology. 2012;137(null):349-442.
https://hdl.handle.net/21.15107/rcub_ibiss_1113 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Miljković, Đorđe, Belij, Sandra, Đokić, Jelena, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats" in Immunology, 137, no. null (2012):349-442,
https://hdl.handle.net/21.15107/rcub_ibiss_1113 .

TY  - JOUR
AU  - Belij, Sandra
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1207
AB  - Contact hypersensitivity reaction (CHS) is a T-cell-mediated skin inflammatory reaction to cutaneous exposure to small sensitizing chemicals, haptens. While the significance of local inflammatory skin response to the hapten application in CHS induction and expression is known, there is paucity of data concerning systemic inflammation in CHS. In this study, changes in cellular (peripheral blood granulocytes) and humoral (plasma tumor necrosis factor (TNF)-alpha levels) components of inflammation during sensitization of rats with two consecutive applications of dinitrochlorobenzene (DNCB) were examined. The impact of sensitization on these parameters was determined by employing low (0.4%) and high (4%) hapten doses and by examining the dynamics (i.e. one and three days following the last application of DNCB) of these changes. Dose-dependent increase in relative numbers and priming (for respiratory burst and adhesion) effect of skin sensitization with DNCB on peripheral blood neutrophils in rats were noted. No changes in circulating TNF-alpha levels were observed following the sensitization. The increase in lung myeloperoxidase content and histologically evident presence of neutrophils was observed in lungs of the sensitized rats. The changes in granulocyte priming for adhesion might have accounted for the observed increase in lung neutrophil content in the sensitized rats.
T2  - Cutaneous and Ocular Toxicology
T1  - Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats
IS  - 1
VL  - 31
EP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1207
ER  - 

@article{
author = "Belij, Sandra and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
abstract = "Contact hypersensitivity reaction (CHS) is a T-cell-mediated skin inflammatory reaction to cutaneous exposure to small sensitizing chemicals, haptens. While the significance of local inflammatory skin response to the hapten application in CHS induction and expression is known, there is paucity of data concerning systemic inflammation in CHS. In this study, changes in cellular (peripheral blood granulocytes) and humoral (plasma tumor necrosis factor (TNF)-alpha levels) components of inflammation during sensitization of rats with two consecutive applications of dinitrochlorobenzene (DNCB) were examined. The impact of sensitization on these parameters was determined by employing low (0.4%) and high (4%) hapten doses and by examining the dynamics (i.e. one and three days following the last application of DNCB) of these changes. Dose-dependent increase in relative numbers and priming (for respiratory burst and adhesion) effect of skin sensitization with DNCB on peripheral blood neutrophils in rats were noted. No changes in circulating TNF-alpha levels were observed following the sensitization. The increase in lung myeloperoxidase content and histologically evident presence of neutrophils was observed in lungs of the sensitized rats. The changes in granulocyte priming for adhesion might have accounted for the observed increase in lung neutrophil content in the sensitized rats.",
journal = "Cutaneous and Ocular Toxicology",
title = "Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats",
number = "1",
volume = "31",
pages = "13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1207"
}

Belij, S., Popov Aleksandrov, A., Mirkov, I., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2012). Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats. in Cutaneous and Ocular Toxicology, 31(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1207

Belij S, Popov Aleksandrov A, Mirkov I, Zolotarevski LD, Kataranovski DS, Kataranovski M. Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats. in Cutaneous and Ocular Toxicology. 2012;31(1):null-13.
https://hdl.handle.net/21.15107/rcub_ibiss_1207 .

Belij, Sandra, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Percutaneous toxicity of dinitrochlorobenzene (DNCB) in rats" in Cutaneous and Ocular Toxicology, 31, no. 1 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1207 .

TY  - JOUR
AU  - Belij, Sandra
AU  - Popov Aleksandrov, Aleksandra
AU  - Zolotarevski, Lidija D
AU  - Mirkov, Ivana
AU  - Đokić, Jelena
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1203
AB  - Topical application of dinitrochlorobenzene (DNCB) is employed in the immunotherapy of skin diseases. Activation of T-cell mediated immune responses (Th1/type1) is the supposed mechanism of the clinical effect of DNCB, but there are no data concerning innate/inflammatory mechanisms. In this study, the effect of repeated topical DNCB application on peripheral blood polymorphonuclear (PMN) leukocytes has been examined in two rat strains which differ in the propensity to mount Th1/type1 or Th2/type2 responses. The dynamics of changes in PMN numbers and effector activities (respiratory burst, nitric oxide production and myeloperoxidase content), as well as in adhesion and TNF-alpha production following the rat skin sensitization with low (0.4%) and high (4%) DNCB doses were measured. Both priming and activation of PMNs were observed following skin sensitization with DNCB, with dose-dependent as well as time-dependent differences in some PMN activities. Obtained data might be relevant for understanding the immune mechanisms of topical DNCB therapy. (C) 2011 Published by Elsevier B.V.
T2  - Environmental Toxicology and Pharmacology
T1  - Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells
IS  - 2
VL  - 33
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1203
ER  - 

@article{
author = "Belij, Sandra and Popov Aleksandrov, Aleksandra and Zolotarevski, Lidija D and Mirkov, Ivana and Đokić, Jelena and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
abstract = "Topical application of dinitrochlorobenzene (DNCB) is employed in the immunotherapy of skin diseases. Activation of T-cell mediated immune responses (Th1/type1) is the supposed mechanism of the clinical effect of DNCB, but there are no data concerning innate/inflammatory mechanisms. In this study, the effect of repeated topical DNCB application on peripheral blood polymorphonuclear (PMN) leukocytes has been examined in two rat strains which differ in the propensity to mount Th1/type1 or Th2/type2 responses. The dynamics of changes in PMN numbers and effector activities (respiratory burst, nitric oxide production and myeloperoxidase content), as well as in adhesion and TNF-alpha production following the rat skin sensitization with low (0.4%) and high (4%) DNCB doses were measured. Both priming and activation of PMNs were observed following skin sensitization with DNCB, with dose-dependent as well as time-dependent differences in some PMN activities. Obtained data might be relevant for understanding the immune mechanisms of topical DNCB therapy. (C) 2011 Published by Elsevier B.V.",
journal = "Environmental Toxicology and Pharmacology",
title = "Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells",
number = "2",
volume = "33",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1203"
}

Belij, S., Popov Aleksandrov, A., Zolotarevski, L. D., Mirkov, I., Đokić, J., Kataranovski, D. S.,& Kataranovski, M.. (2012). Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells. in Environmental Toxicology and Pharmacology, 33(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1203

Belij S, Popov Aleksandrov A, Zolotarevski LD, Mirkov I, Đokić J, Kataranovski DS, Kataranovski M. Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells. in Environmental Toxicology and Pharmacology. 2012;33(2):null-180.
https://hdl.handle.net/21.15107/rcub_ibiss_1203 .

Belij, Sandra, Popov Aleksandrov, Aleksandra, Zolotarevski, Lidija D, Mirkov, Ivana, Đokić, Jelena, Kataranovski, Dragan S., Kataranovski, Milena, "Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells" in Environmental Toxicology and Pharmacology, 33, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1203 .

TY  - JOUR
AU  - Belij, Sandra
AU  - Miljković, Đorđe
AU  - Popov Aleksandrov, Aleksandra
AU  - Subota, Vesna S
AU  - Timotijević, Gordana S
AU  - Slavić, Marija
AU  - Mirkov, Ivana
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1185
AB  - Warfarin affects mainly vitamin K dependent (VKD) processes, but the effects on some non-VKD-related activities such as tumor growth inhibition and mononuclear cell-mediated immune reactions were shown as well. In this study, the effect of subchronic (30 days) oral warfarin (0.35 mg/l and 3.5 mg/l) intake on peripheral blood granulocytes in rats was investigated. Increase in prothrombin and partial thromboplastin time at high warfarin dose reflected its basic activity. Priming effect for respiratory burst was noted at both warfarin doses, while only high warfarin dose resulted in priming for adhesion, the rise in intracellular myeloperoxidase content/release and stimulation of nitric oxide production. Differential effects of high warfarin dose were noted on granulocyte cytokines IL-6 (lack of the effect), TNF-alpha (decreased release and mRNA expression) and IL-12 (increase in mRNA for IL-12 subunits p35 and p40). Changes in granulocytes seems not to rely on mitogen activated kinases p38 and ERK. Warfarin intake was associated with an increase in circulating IL-6, fibrinogen and haptoglobin and with changes in the activity of erythrocyte antioxidant enzymes superoxide dismutase and catalase. The effects of oral warfarin intake on peripheral blood granulocytes demonstrated in this study might be relevant for oral anticoagulant therapy strategies in humans. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats
IS  - 5
VL  - 50
EP  - 1507
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1185
ER  - 

@article{
author = "Belij, Sandra and Miljković, Đorđe and Popov Aleksandrov, Aleksandra and Subota, Vesna S and Timotijević, Gordana S and Slavić, Marija and Mirkov, Ivana and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
abstract = "Warfarin affects mainly vitamin K dependent (VKD) processes, but the effects on some non-VKD-related activities such as tumor growth inhibition and mononuclear cell-mediated immune reactions were shown as well. In this study, the effect of subchronic (30 days) oral warfarin (0.35 mg/l and 3.5 mg/l) intake on peripheral blood granulocytes in rats was investigated. Increase in prothrombin and partial thromboplastin time at high warfarin dose reflected its basic activity. Priming effect for respiratory burst was noted at both warfarin doses, while only high warfarin dose resulted in priming for adhesion, the rise in intracellular myeloperoxidase content/release and stimulation of nitric oxide production. Differential effects of high warfarin dose were noted on granulocyte cytokines IL-6 (lack of the effect), TNF-alpha (decreased release and mRNA expression) and IL-12 (increase in mRNA for IL-12 subunits p35 and p40). Changes in granulocytes seems not to rely on mitogen activated kinases p38 and ERK. Warfarin intake was associated with an increase in circulating IL-6, fibrinogen and haptoglobin and with changes in the activity of erythrocyte antioxidant enzymes superoxide dismutase and catalase. The effects of oral warfarin intake on peripheral blood granulocytes demonstrated in this study might be relevant for oral anticoagulant therapy strategies in humans. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats",
number = "5",
volume = "50",
pages = "1507",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1185"
}

Belij, S., Miljković, Đ., Popov Aleksandrov, A., Subota, V. S., Timotijević, G. S., Slavić, M., Mirkov, I., Kataranovski, D. S.,& Kataranovski, M.. (2012). Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats. in Food and Chemical Toxicology, 50(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1185

Belij S, Miljković Đ, Popov Aleksandrov A, Subota VS, Timotijević GS, Slavić M, Mirkov I, Kataranovski DS, Kataranovski M. Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats. in Food and Chemical Toxicology. 2012;50(5):null-1507.
https://hdl.handle.net/21.15107/rcub_ibiss_1185 .

Belij, Sandra, Miljković, Đorđe, Popov Aleksandrov, Aleksandra, Subota, Vesna S, Timotijević, Gordana S, Slavić, Marija, Mirkov, Ivana, Kataranovski, Dragan S., Kataranovski, Milena, "Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats" in Food and Chemical Toxicology, 50, no. 5 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1185 .

TY  - CONF
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - El-Muzghi, Amal Atia Mhfuod
AU  - Glamočlija, Jasmina
AU  - Miljković, Đorđe
AU  - Pokić, J
AU  - Ninkov, Marina
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1118
UR  - https://onlinelibrary.wiley.com/toc/13652567/2012/137/s1
C3  - Immunology
T1  - Differential mechanisms of resistance to pulmonary Aspergillus fumigatus infection in immunocompetent rats
IS  - null
VL  - 137
SP  - 137
EP  - 732
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1118
ER  - 

@conference{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and El-Muzghi, Amal Atia Mhfuod and Glamočlija, Jasmina and Miljković, Đorđe and Pokić, J and Ninkov, Marina and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
journal = "Immunology",
title = "Differential mechanisms of resistance to pulmonary Aspergillus fumigatus infection in immunocompetent rats",
number = "null",
volume = "137",
pages = "137-732",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1118"
}

Popov Aleksandrov, A., Mirkov, I., El-Muzghi, A. A. M., Glamočlija, J., Miljković, Đ., Pokić, J., Ninkov, M., Kataranovski, D. S.,& Kataranovski, M.. (2012). Differential mechanisms of resistance to pulmonary Aspergillus fumigatus infection in immunocompetent rats. in Immunology, 137(null), 137-732.
https://hdl.handle.net/21.15107/rcub_ibiss_1118

Popov Aleksandrov A, Mirkov I, El-Muzghi AAM, Glamočlija J, Miljković Đ, Pokić J, Ninkov M, Kataranovski DS, Kataranovski M. Differential mechanisms of resistance to pulmonary Aspergillus fumigatus infection in immunocompetent rats. in Immunology. 2012;137(null):137-732.
https://hdl.handle.net/21.15107/rcub_ibiss_1118 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, El-Muzghi, Amal Atia Mhfuod, Glamočlija, Jasmina, Miljković, Đorđe, Pokić, J, Ninkov, Marina, Kataranovski, Dragan S., Kataranovski, Milena, "Differential mechanisms of resistance to pulmonary Aspergillus fumigatus infection in immunocompetent rats" in Immunology, 137, no. null (2012):137-732,
https://hdl.handle.net/21.15107/rcub_ibiss_1118 .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Zolotarevski, Lidija D
AU  - Jović, Milena
AU  - Belij, Sandra
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1299
AB  - Objective: To evaluate the effects of epicutaneous application of anticoagulant warfarin, by examining the presence of tissue injury and immune/inflammatory activity in exposed skin. Methods: Rats were exposed to warfarin by applying 10 mu g of warfarin-sodium to 10-12 cm(2) skin (range 0.8-1 mu g per 1 cm(2)) for 3 consecutive days. Tissue injury was evaluated by lipid peroxidation, histomorphological changes and signs of reparative activity in skin. T cell infiltration and selected aspects of epidermal cell activity were examined as indicators of immune/inflammatory skin response to warfarin application. Results: Repeated warfarin application exerted no effect on skin metabolic viability, but resulted in tissue injury (increased malondialdehyde, MDA, production, evident histo-morphological changes in epidermis and dermis depicting cell injury and death). Increased numbers of proliferating cell nuclear antigen (PCNA(+)) cells indicated reparative processes in injured skin. Infiltration of CD3(+) cells (T lymphocytes) along with the increased production of tumor necrosis factor-alpha (TNF-alpha) by epidermal cells from warfarin-treated skin and their co-stimulatory effect in an in vitro T-cell activation assay demonstrated immunomodulatory effects of epicutaneous warfarin. Conclusion: Presented data have documented tissue damage associated with immune/inflammatory activity in skin exposed to warfarin. Observed effects are relevant to immunotoxic potential of this anticoagulant in settings of external exposure.
T2  - Biomedical and Environmental Sciences
T1  - Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats
IS  - 2
VL  - 24
EP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1299
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Zolotarevski, Lidija D and Jović, Milena and Belij, Sandra and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2011",
abstract = "Objective: To evaluate the effects of epicutaneous application of anticoagulant warfarin, by examining the presence of tissue injury and immune/inflammatory activity in exposed skin. Methods: Rats were exposed to warfarin by applying 10 mu g of warfarin-sodium to 10-12 cm(2) skin (range 0.8-1 mu g per 1 cm(2)) for 3 consecutive days. Tissue injury was evaluated by lipid peroxidation, histomorphological changes and signs of reparative activity in skin. T cell infiltration and selected aspects of epidermal cell activity were examined as indicators of immune/inflammatory skin response to warfarin application. Results: Repeated warfarin application exerted no effect on skin metabolic viability, but resulted in tissue injury (increased malondialdehyde, MDA, production, evident histo-morphological changes in epidermis and dermis depicting cell injury and death). Increased numbers of proliferating cell nuclear antigen (PCNA(+)) cells indicated reparative processes in injured skin. Infiltration of CD3(+) cells (T lymphocytes) along with the increased production of tumor necrosis factor-alpha (TNF-alpha) by epidermal cells from warfarin-treated skin and their co-stimulatory effect in an in vitro T-cell activation assay demonstrated immunomodulatory effects of epicutaneous warfarin. Conclusion: Presented data have documented tissue damage associated with immune/inflammatory activity in skin exposed to warfarin. Observed effects are relevant to immunotoxic potential of this anticoagulant in settings of external exposure.",
journal = "Biomedical and Environmental Sciences",
title = "Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats",
number = "2",
volume = "24",
pages = "189",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1299"
}

Popov Aleksandrov, A., Mirkov, I., Zolotarevski, L. D., Jović, M., Belij, S., Kataranovski, D. S.,& Kataranovski, M.. (2011). Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats. in Biomedical and Environmental Sciences, 24(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1299

Popov Aleksandrov A, Mirkov I, Zolotarevski LD, Jović M, Belij S, Kataranovski DS, Kataranovski M. Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats. in Biomedical and Environmental Sciences. 2011;24(2):null-189.
https://hdl.handle.net/21.15107/rcub_ibiss_1299 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Zolotarevski, Lidija D, Jović, Milena, Belij, Sandra, Kataranovski, Dragan S., Kataranovski, Milena, "Local Proinflammatory Effects of Repeated Skin Exposure to Warfarin, An Anticoagulant Rodenticide in Rats" in Biomedical and Environmental Sciences, 24, no. 2 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1299 .

TY  - JOUR
AU  - Kataranovski, Milena
AU  - Mirkov, Ivana
AU  - Belij, Sandra
AU  - Popov Aleksandrov, Aleksandra
AU  - Petrović, Z
AU  - Gacić, Zoran M
AU  - Kataranovski, Dragan S.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1291
AB  - Gastrointestinal helminths of Norway rat (Rattus norvegicus) from the Belgrade area were studied as a part of a wider ecological research of rats in Serbia (data on the distribution, population ecology, economic and epizoothiological-epidemiological importance, and density control). Rats were captured from May 2005 to July 2009 at both urban and suburban-rural sites. Of a total of 302 trapped rats 48 % were males and 52 % females, with 36.5 % and 38.8 % of juvenile-subadult individuals, per sex respectively. Intestinal helminth infection was noted in 68.5 % of rats, with a higher prevalence in male hosts and in adult individuals. Higher numbers of infected juveniles-subadults were noted in suburban-rural habitats, while an opposite tendency was noted in adult rats. Seven helminth species were recovered, of which five were nematode (Heterakis spumosa, Nippostrongylus brasiliensis, Capillaria sp., Trichuris muris and Syphacia muris) and two cestode species (Hymenolepis diminuta and Rodentolepis fraterna). The most prevalent parasites were Heterakis spumosa (36.7 %) and Hymenolepis diminuta (30.5 %). Sex and habitat-related differences were noted in the prevalence of infection with Capillaria sp. and Trichuris muris, while there were no age-related differences in the prevalence of infection with any individual helminth species. Significantly higher prevalence of infection was noted in summer as compared to spring or winter, with a tendency to be higher in autumn as compared to spring. The only significant difference in the prevalence of infection between habitat-related was noted during spring. H. spumosa was most prevalent in summer, while H. diminuta and N. brasiliensis in autumn. The mean intensity of infection with H. spumosa, R. fraterna, S. muris and I muris was higher in autumn than in the other seasons, while N. brasiliensis and Capillaria sp. occured in winter. No more than four helminth species were found in one host.
T2  - Parasite-Journal de La Societe Francaise de Parasitologie
T1  - Intestinal Helminths Infection of Rats (Ratus Norvegicus) in the Belgrade Area (Serbia): the Effect of Sex, Age and Habitat
IS  - 2
VL  - 18
EP  - 196
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1291
ER  - 

@article{
author = "Kataranovski, Milena and Mirkov, Ivana and Belij, Sandra and Popov Aleksandrov, Aleksandra and Petrović, Z and Gacić, Zoran M and Kataranovski, Dragan S.",
year = "2011",
abstract = "Gastrointestinal helminths of Norway rat (Rattus norvegicus) from the Belgrade area were studied as a part of a wider ecological research of rats in Serbia (data on the distribution, population ecology, economic and epizoothiological-epidemiological importance, and density control). Rats were captured from May 2005 to July 2009 at both urban and suburban-rural sites. Of a total of 302 trapped rats 48 % were males and 52 % females, with 36.5 % and 38.8 % of juvenile-subadult individuals, per sex respectively. Intestinal helminth infection was noted in 68.5 % of rats, with a higher prevalence in male hosts and in adult individuals. Higher numbers of infected juveniles-subadults were noted in suburban-rural habitats, while an opposite tendency was noted in adult rats. Seven helminth species were recovered, of which five were nematode (Heterakis spumosa, Nippostrongylus brasiliensis, Capillaria sp., Trichuris muris and Syphacia muris) and two cestode species (Hymenolepis diminuta and Rodentolepis fraterna). The most prevalent parasites were Heterakis spumosa (36.7 %) and Hymenolepis diminuta (30.5 %). Sex and habitat-related differences were noted in the prevalence of infection with Capillaria sp. and Trichuris muris, while there were no age-related differences in the prevalence of infection with any individual helminth species. Significantly higher prevalence of infection was noted in summer as compared to spring or winter, with a tendency to be higher in autumn as compared to spring. The only significant difference in the prevalence of infection between habitat-related was noted during spring. H. spumosa was most prevalent in summer, while H. diminuta and N. brasiliensis in autumn. The mean intensity of infection with H. spumosa, R. fraterna, S. muris and I muris was higher in autumn than in the other seasons, while N. brasiliensis and Capillaria sp. occured in winter. No more than four helminth species were found in one host.",
journal = "Parasite-Journal de La Societe Francaise de Parasitologie",
title = "Intestinal Helminths Infection of Rats (Ratus Norvegicus) in the Belgrade Area (Serbia): the Effect of Sex, Age and Habitat",
number = "2",
volume = "18",
pages = "196",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1291"
}

Kataranovski, M., Mirkov, I., Belij, S., Popov Aleksandrov, A., Petrović, Z., Gacić, Z. M.,& Kataranovski, D. S.. (2011). Intestinal Helminths Infection of Rats (Ratus Norvegicus) in the Belgrade Area (Serbia): the Effect of Sex, Age and Habitat. in Parasite-Journal de La Societe Francaise de Parasitologie, 18(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1291

Kataranovski M, Mirkov I, Belij S, Popov Aleksandrov A, Petrović Z, Gacić ZM, Kataranovski DS. Intestinal Helminths Infection of Rats (Ratus Norvegicus) in the Belgrade Area (Serbia): the Effect of Sex, Age and Habitat. in Parasite-Journal de La Societe Francaise de Parasitologie. 2011;18(2):null-196.
https://hdl.handle.net/21.15107/rcub_ibiss_1291 .

Kataranovski, Milena, Mirkov, Ivana, Belij, Sandra, Popov Aleksandrov, Aleksandra, Petrović, Z, Gacić, Zoran M, Kataranovski, Dragan S., "Intestinal Helminths Infection of Rats (Ratus Norvegicus) in the Belgrade Area (Serbia): the Effect of Sex, Age and Habitat" in Parasite-Journal de La Societe Francaise de Parasitologie, 18, no. 2 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1291 .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Miljković, Đorđe
AU  - Belij, Sandra
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1278
AB  - Contact hypersensitivity (CHS) is a T-cell-mediated skin inflammatory reaction to cutaneous exposure to small sensitizing chemicals, haptens. Majority of CHS studies were conducted in mice and there is paucity of data in other experimental animals. In the present study, characteristics of contact hypersensitivity reaction to dinitrochlorobenzene (DNCB) were determined in Th1-prone Dark Agouti (DA) rats by evaluating sensitization phase as a function of time-dependent changes in draining lymph nodes (DLN). Apart from basic indices of DLN activity (cellularity and proliferation), the production of cytokines relevant for CHS induction, interleukin-6 (IL-6), interferon-gamma (IFN-gamma), interleukin-17 (IL-17) and interleukin-4 (IL-4) was analyzed. Anti-inflammatory cytokine interleukin-10 (IL-10) production by DLN cells was determined as well. Highest production of IL-6, IFN-gamma and IL-17 in sensitized animals was observed at day 3 after DNCB application, with a decrease at day 5. Increased messages for IFN-gamma and IL-17 were noted at this time point. In contrast to inflammatory cytokines, anti-inflammatory cytokine interleukin-4 (IL-4) was undetectable during the entire sensitization phase. Differential pattern (IL-6 and IFN-gamma) and level (IFN-gamma and IL-17) of inflammatory cytokine production was noted in sensitized Th2-prone Albino Oxford (AO) rats. Similarly to DA rats, no changes in IL-4 were noted in AO rats. Strain-dependent differences in inflammatory cytokine production seem to be based on anti-inflammatory cytokine interleukin-10 (IL-10). Production of IFN-gamma concomitantly with undetectable IL-4 in both strains classify rat CHS to DNCB as Th1/type 1 reaction. Detection of IL-17 in sensitized DLN cells points to the involvement of T(IL-17) cells in rat contact hypersensitivity. (C) 2011 Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase
IS  - 7
VL  - 216
EP  - 770
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1278
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Miljković, Đorđe and Belij, Sandra and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2011",
abstract = "Contact hypersensitivity (CHS) is a T-cell-mediated skin inflammatory reaction to cutaneous exposure to small sensitizing chemicals, haptens. Majority of CHS studies were conducted in mice and there is paucity of data in other experimental animals. In the present study, characteristics of contact hypersensitivity reaction to dinitrochlorobenzene (DNCB) were determined in Th1-prone Dark Agouti (DA) rats by evaluating sensitization phase as a function of time-dependent changes in draining lymph nodes (DLN). Apart from basic indices of DLN activity (cellularity and proliferation), the production of cytokines relevant for CHS induction, interleukin-6 (IL-6), interferon-gamma (IFN-gamma), interleukin-17 (IL-17) and interleukin-4 (IL-4) was analyzed. Anti-inflammatory cytokine interleukin-10 (IL-10) production by DLN cells was determined as well. Highest production of IL-6, IFN-gamma and IL-17 in sensitized animals was observed at day 3 after DNCB application, with a decrease at day 5. Increased messages for IFN-gamma and IL-17 were noted at this time point. In contrast to inflammatory cytokines, anti-inflammatory cytokine interleukin-4 (IL-4) was undetectable during the entire sensitization phase. Differential pattern (IL-6 and IFN-gamma) and level (IFN-gamma and IL-17) of inflammatory cytokine production was noted in sensitized Th2-prone Albino Oxford (AO) rats. Similarly to DA rats, no changes in IL-4 were noted in AO rats. Strain-dependent differences in inflammatory cytokine production seem to be based on anti-inflammatory cytokine interleukin-10 (IL-10). Production of IFN-gamma concomitantly with undetectable IL-4 in both strains classify rat CHS to DNCB as Th1/type 1 reaction. Detection of IL-17 in sensitized DLN cells points to the involvement of T(IL-17) cells in rat contact hypersensitivity. (C) 2011 Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase",
number = "7",
volume = "216",
pages = "770",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1278"
}

Popov Aleksandrov, A., Mirkov, I., Miljković, Đ., Belij, S., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2011). Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase. in Immunobiology, 216(7).
https://hdl.handle.net/21.15107/rcub_ibiss_1278

Popov Aleksandrov A, Mirkov I, Miljković Đ, Belij S, Zolotarevski LD, Kataranovski DS, Kataranovski M. Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase. in Immunobiology. 2011;216(7):null-770.
https://hdl.handle.net/21.15107/rcub_ibiss_1278 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Miljković, Đorđe, Belij, Sandra, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Contact allergic response to dinitrochlorobenzene (DNCB) in rats: Insight from sensitization phase" in Immunobiology, 216, no. 7 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1278 .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Stojanović, Ivana D.
AU  - Glamočlija, Jasmina
AU  - Stošić-Grujičić, Stanislava
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1327
AB  - Studies of systemic and pulmonary Aspergillus fumigatus infection demonstrated differential susceptibility of inbred mice of various genetic background to lethal outcome, with an opposite pattern of Th1 cytokine interferon-gamma (IFN-gamma) and Th2 cytokine interleukin-4 (IL-4) in susceptible vs resistant mice. We have shown recently reciprocal IFN-gamma and IL-4 expression in spleens of Th1 -prone C57BL/6 mice in sublethal systemic aspergillosis. In this study, resistance to systemic (i.v.) A. fumigatus infection was investigated in Th2-prone BALB/c mice by survival rate at different fungal inocula, efficiency of reduction of visceral organ and spleen fungal burden at sublethal conidia dose and splenic immune response to this dose and compared to C57BL/6 mice. No strain differences in survival were noted at three A. fumigatus doses, with similar extent and dynamics of fungal eradication from all organs following sublethal conidia dose injection. Progressive decrease in spleen fungal burden was associated with different dynamics and quality of changes in spleen activity of BALB/c and C57BL/6 mice. Increased spleen mass and cellularity was noted in both strains, with higher values in BALB/c mice at some time points what might be ascribed to peripheral blood cell recruitment, as well as hematopoietic activity and red pulp upgrowth. Infection tipped the balance towards pro-inflammatory antifungal splenic response by a highly increasing IFN-gamma and without changing the IL-4 expression in BALB/c mice, in contrast to down-regulating anti-inflammatory (IL-4) and a moderately increasing IFN-gamma response in C57BL/6 mice. Jointly, stimulation of IL-17 expression noted in both strains provided an optimal inflammatory milieu in the spleen of infected mice that might have contributed to efficient removal of conidia. (C) 2010 Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice
IS  - 1-2
VL  - 216
EP  - 242
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1327
ER  - 

@article{
author = "Mirkov, Ivana and Stojanović, Ivana D. and Glamočlija, Jasmina and Stošić-Grujičić, Stanislava and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2011",
abstract = "Studies of systemic and pulmonary Aspergillus fumigatus infection demonstrated differential susceptibility of inbred mice of various genetic background to lethal outcome, with an opposite pattern of Th1 cytokine interferon-gamma (IFN-gamma) and Th2 cytokine interleukin-4 (IL-4) in susceptible vs resistant mice. We have shown recently reciprocal IFN-gamma and IL-4 expression in spleens of Th1 -prone C57BL/6 mice in sublethal systemic aspergillosis. In this study, resistance to systemic (i.v.) A. fumigatus infection was investigated in Th2-prone BALB/c mice by survival rate at different fungal inocula, efficiency of reduction of visceral organ and spleen fungal burden at sublethal conidia dose and splenic immune response to this dose and compared to C57BL/6 mice. No strain differences in survival were noted at three A. fumigatus doses, with similar extent and dynamics of fungal eradication from all organs following sublethal conidia dose injection. Progressive decrease in spleen fungal burden was associated with different dynamics and quality of changes in spleen activity of BALB/c and C57BL/6 mice. Increased spleen mass and cellularity was noted in both strains, with higher values in BALB/c mice at some time points what might be ascribed to peripheral blood cell recruitment, as well as hematopoietic activity and red pulp upgrowth. Infection tipped the balance towards pro-inflammatory antifungal splenic response by a highly increasing IFN-gamma and without changing the IL-4 expression in BALB/c mice, in contrast to down-regulating anti-inflammatory (IL-4) and a moderately increasing IFN-gamma response in C57BL/6 mice. Jointly, stimulation of IL-17 expression noted in both strains provided an optimal inflammatory milieu in the spleen of infected mice that might have contributed to efficient removal of conidia. (C) 2010 Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice",
number = "1-2",
volume = "216",
pages = "242",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1327"
}

Mirkov, I., Stojanović, I. D., Glamočlija, J., Stošić-Grujičić, S., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2011). Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice. in Immunobiology, 216(1-2).
https://hdl.handle.net/21.15107/rcub_ibiss_1327

Mirkov I, Stojanović ID, Glamočlija J, Stošić-Grujičić S, Zolotarevski LD, Kataranovski DS, Kataranovski M. Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice. in Immunobiology. 2011;216(1-2):null-242.
https://hdl.handle.net/21.15107/rcub_ibiss_1327 .

Mirkov, Ivana, Stojanović, Ivana D., Glamočlija, Jasmina, Stošić-Grujičić, Stanislava, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Differential mechanisms of resistance to sublethal systemic Aspergillus fumigatus infection in immunocompetent BALB/c and C57BL/6 mice" in Immunobiology, 216, no. 1-2 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1327 .

TY  - JOUR
AU  - Vujanić, Marija Z
AU  - Ivović, Vladimir
AU  - Kataranovski, Milena
AU  - Nikolić, Aleksandra I
AU  - Bobić, Branko N
AU  - Klun, Ivana
AU  - Villena, Isabelle
AU  - Kataranovski, Dragan S.
AU  - Đurković-Đaković, Olgica M
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1273
AB  - To assess the role of synanthropic rodents in the epidemiology of urban toxoplasmosis, Toxoplasma gondii infection was examined in 144 rats (Rattus norvegicus) and 12 mice (Mus musculus) captured using live animal traps in three locations in Belgrade city characterized by poor housing and degraded environment. In rats, specific IgG antibodies were detected by modified agglutination test in 22 (27.5%) of the 80 blood samples available. Toxoplasma brain cysts were microscopically detected in 11 (7.6%), and Toxoplasma DNA by real-time polymerase chain reaction was demonstrated in 15 (10.4%) animals. Of these, both cysts and Toxoplasma DNA were detected in five (3.5%) rats. In mice, cysts were observed in 3 (25%), but Toxoplasma DNA was detected in even 10 (83.3%) animals, including all 3 with morphologically recognized cysts. Being a link in the chain of Toxoplasma infection, the existence of urban rodent reservoirs of infection represents a public health risk.
T2  - Vector-Borne and Zoonotic Diseases
T1  - Toxoplasmosis in Naturally Infected Rodents in Belgrade, Serbia
IS  - 8
VL  - 11
EP  - 1211
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1273
ER  - 

@article{
author = "Vujanić, Marija Z and Ivović, Vladimir and Kataranovski, Milena and Nikolić, Aleksandra I and Bobić, Branko N and Klun, Ivana and Villena, Isabelle and Kataranovski, Dragan S. and Đurković-Đaković, Olgica M",
year = "2011",
abstract = "To assess the role of synanthropic rodents in the epidemiology of urban toxoplasmosis, Toxoplasma gondii infection was examined in 144 rats (Rattus norvegicus) and 12 mice (Mus musculus) captured using live animal traps in three locations in Belgrade city characterized by poor housing and degraded environment. In rats, specific IgG antibodies were detected by modified agglutination test in 22 (27.5%) of the 80 blood samples available. Toxoplasma brain cysts were microscopically detected in 11 (7.6%), and Toxoplasma DNA by real-time polymerase chain reaction was demonstrated in 15 (10.4%) animals. Of these, both cysts and Toxoplasma DNA were detected in five (3.5%) rats. In mice, cysts were observed in 3 (25%), but Toxoplasma DNA was detected in even 10 (83.3%) animals, including all 3 with morphologically recognized cysts. Being a link in the chain of Toxoplasma infection, the existence of urban rodent reservoirs of infection represents a public health risk.",
journal = "Vector-Borne and Zoonotic Diseases",
title = "Toxoplasmosis in Naturally Infected Rodents in Belgrade, Serbia",
number = "8",
volume = "11",
pages = "1211",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1273"
}

Vujanić, M. Z., Ivović, V., Kataranovski, M., Nikolić, A. I., Bobić, B. N., Klun, I., Villena, I., Kataranovski, D. S.,& Đurković-Đaković, O. M.. (2011). Toxoplasmosis in Naturally Infected Rodents in Belgrade, Serbia. in Vector-Borne and Zoonotic Diseases, 11(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1273

Vujanić MZ, Ivović V, Kataranovski M, Nikolić AI, Bobić BN, Klun I, Villena I, Kataranovski DS, Đurković-Đaković OM. Toxoplasmosis in Naturally Infected Rodents in Belgrade, Serbia. in Vector-Borne and Zoonotic Diseases. 2011;11(8):null-1211.
https://hdl.handle.net/21.15107/rcub_ibiss_1273 .

Vujanić, Marija Z, Ivović, Vladimir, Kataranovski, Milena, Nikolić, Aleksandra I, Bobić, Branko N, Klun, Ivana, Villena, Isabelle, Kataranovski, Dragan S., Đurković-Đaković, Olgica M, "Toxoplasmosis in Naturally Infected Rodents in Belgrade, Serbia" in Vector-Borne and Zoonotic Diseases, 11, no. 8 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1273 .

TY  - JOUR
AU  - Stošić, Jelena
AU  - Mirkov, Ivana
AU  - Belij, Sandra
AU  - Nikolić, Miroslav
AU  - Popov Aleksandrov, Aleksandra
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1355
AB  - Objective To examine the presence of gender differences in pulmonary inflammation evoked by acute systemic cadmium administration in rats. Methods Presence of basic indicators of lung inflammation (inflammatory cytokine lung content, leukocyte infiltration and activity of cells recovered from lungs by enzyme digestion) was analyzed and compared in animals of the two sexes. Results Intraperitoneal administration of cadmium (1.0 mg/kg) resulted in higher cadmium content in lungs of female rats. Higher tumor necrosis factor (TNF) content was noted in lung homogenates of male rats, while interleukin-6 (IL-6) content was slightly, but signifaicantly greater in lungs of female rats. Increased leukocyte infiltration was observed in lungs of male rats, mainly due to neutrophils. Increased responsiveness to phorbol myristate acetate (PMA) stimulation was noted in cells recovered from lungs of male rats. Rise in intracellular content of myeloperoxidase (MPO) was noted in lung cells from cadmium-treated rats of both sexes, but higher in cells from male rats. Conclusion Presented data documented a more intense pulmonary inflammatory response to systemic cadmium administration in males, with higher IL-6 levels in lungs of female individuals. These sex differences in proinflamatory activity of cadmium in lungs should be taken into consideration in studying the remote toxicity of this heavy metal.
T2  - Biomedical and Environmental Sciences
T1  - Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats
IS  - 4
VL  - 23
EP  - 299
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1355
ER  - 

@article{
author = "Stošić, Jelena and Mirkov, Ivana and Belij, Sandra and Nikolić, Miroslav and Popov Aleksandrov, Aleksandra and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2010",
abstract = "Objective To examine the presence of gender differences in pulmonary inflammation evoked by acute systemic cadmium administration in rats. Methods Presence of basic indicators of lung inflammation (inflammatory cytokine lung content, leukocyte infiltration and activity of cells recovered from lungs by enzyme digestion) was analyzed and compared in animals of the two sexes. Results Intraperitoneal administration of cadmium (1.0 mg/kg) resulted in higher cadmium content in lungs of female rats. Higher tumor necrosis factor (TNF) content was noted in lung homogenates of male rats, while interleukin-6 (IL-6) content was slightly, but signifaicantly greater in lungs of female rats. Increased leukocyte infiltration was observed in lungs of male rats, mainly due to neutrophils. Increased responsiveness to phorbol myristate acetate (PMA) stimulation was noted in cells recovered from lungs of male rats. Rise in intracellular content of myeloperoxidase (MPO) was noted in lung cells from cadmium-treated rats of both sexes, but higher in cells from male rats. Conclusion Presented data documented a more intense pulmonary inflammatory response to systemic cadmium administration in males, with higher IL-6 levels in lungs of female individuals. These sex differences in proinflamatory activity of cadmium in lungs should be taken into consideration in studying the remote toxicity of this heavy metal.",
journal = "Biomedical and Environmental Sciences",
title = "Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats",
number = "4",
volume = "23",
pages = "299",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1355"
}

Stošić, J., Mirkov, I., Belij, S., Nikolić, M., Popov Aleksandrov, A., Kataranovski, D. S.,& Kataranovski, M.. (2010). Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats. in Biomedical and Environmental Sciences, 23(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1355

Stošić J, Mirkov I, Belij S, Nikolić M, Popov Aleksandrov A, Kataranovski DS, Kataranovski M. Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats. in Biomedical and Environmental Sciences. 2010;23(4):null-299.
https://hdl.handle.net/21.15107/rcub_ibiss_1355 .

Stošić, Jelena, Mirkov, Ivana, Belij, Sandra, Nikolić, Miroslav, Popov Aleksandrov, Aleksandra, Kataranovski, Dragan S., Kataranovski, Milena, "Gender Differences in Pulmonary Inflammation Following Systemic Cadmium Administration in Rats" in Biomedical and Environmental Sciences, 23, no. 4 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1355 .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Stojanović, Ivana D.
AU  - Stošić-Grujičić, Stanislava
AU  - Glamočlija, Jasmina
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1359
AB  - In this study, we investigated splenic and lung cell responses to nonlethal systemic Aspergillus fumigatus infection in mice. Apart from basic indices of spleen and lung cell activity, IL-17 expression by cells from both tissues was determined and compared to the expression of IFN-gamma and IL-4. Progressive decrease in tissue fungal burden correlated with increased spleen and lung cell activity. Increased IL-17 message was noted in spleen cells at all time points (3, 7 and 15 days post-infection; p.i.), while a modest increase in IFN-gamma mRNA expression was noted at day 3 p.i. Increased cytokine production at days 3 and 7 (IL-17) and throughout the experimental period (IFN-gamma) was found. In contrast, spleen cell IL-4 expression was considerably lower during infection, resulting in high IFN-gamma/IL-4 and IL-17/IL-4 ratios in the spleen. Pro-inflammatory cytokine response was observed in the lungs as well, but primarily as the result of increased production of IFN-gamma by lung cells in response to challenge with conidia and the absence of change in IL-4 response. Increased activity of cells from both tissues, as well as the pattern of cytokine production, created an optimal pro-inflammatory milieu for fungal eradication.
T2  - Medical Mycology
T1  - Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice
IS  - 5
VL  - 48
EP  - 743
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1359
ER  - 

@article{
author = "Mirkov, Ivana and Stojanović, Ivana D. and Stošić-Grujičić, Stanislava and Glamočlija, Jasmina and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2010",
abstract = "In this study, we investigated splenic and lung cell responses to nonlethal systemic Aspergillus fumigatus infection in mice. Apart from basic indices of spleen and lung cell activity, IL-17 expression by cells from both tissues was determined and compared to the expression of IFN-gamma and IL-4. Progressive decrease in tissue fungal burden correlated with increased spleen and lung cell activity. Increased IL-17 message was noted in spleen cells at all time points (3, 7 and 15 days post-infection; p.i.), while a modest increase in IFN-gamma mRNA expression was noted at day 3 p.i. Increased cytokine production at days 3 and 7 (IL-17) and throughout the experimental period (IFN-gamma) was found. In contrast, spleen cell IL-4 expression was considerably lower during infection, resulting in high IFN-gamma/IL-4 and IL-17/IL-4 ratios in the spleen. Pro-inflammatory cytokine response was observed in the lungs as well, but primarily as the result of increased production of IFN-gamma by lung cells in response to challenge with conidia and the absence of change in IL-4 response. Increased activity of cells from both tissues, as well as the pattern of cytokine production, created an optimal pro-inflammatory milieu for fungal eradication.",
journal = "Medical Mycology",
title = "Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice",
number = "5",
volume = "48",
pages = "743",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1359"
}

Mirkov, I., Stojanović, I. D., Stošić-Grujičić, S., Glamočlija, J., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2010). Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice. in Medical Mycology, 48(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1359

Mirkov I, Stojanović ID, Stošić-Grujičić S, Glamočlija J, Zolotarevski LD, Kataranovski DS, Kataranovski M. Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice. in Medical Mycology. 2010;48(5):null-743.
https://hdl.handle.net/21.15107/rcub_ibiss_1359 .

Mirkov, Ivana, Stojanović, Ivana D., Stošić-Grujičić, Stanislava, Glamočlija, Jasmina, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Splenic and lung response to nonlethal systemic Aspergillus fumigatus infection in C57BL/6 mice" in Medical Mycology, 48, no. 5 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1359 .

TY  - JOUR
AU  - Kataranovski, Milena
AU  - Janković, Srđa J
AU  - Kataranovski, Dragan S.
AU  - Stošić, Jelena
AU  - Bogojević, Desanka
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1464
AB  - To examine the presence of gender differences in pro-inflammatory potential of cadmium in rats by comparing systemic inflammatory response to acute cadmium intoxication in animals of the two sexes. Methods Basic aspects of this response were evaluated, including plasma levels of inflammatory cytokines tumor necrosis factor (TNF) and interleukin-6 (IL-6) and of major rat acute phase protein alpha 2-macroglobulin (alpha2-M), as Soluble indicators of inflammation, and the number and activity of peripheral blood leukocytes, as cellular indicators of inflammation. Results Differential increases of IL-6 and alpha 2-M (higher in males than in females) in peripheral blood cell counts and types (leukocytosis and shift in the ratio of granulocytes to lymphocytes more pronounced in males vs females) and in levels of neutrophil priming (higher in males vs females) were noted. Conclusion The data document a more intense inflammatory response to cadmium administration in males. The sex differences in inflammatory effects of cadmium might be taken into consideration in studying the toxicity of this heavy metal.
T2  - Biomedical and Environmental Sciences
T1  - Gender Differences in Acute Cadmium-Induced Systemic Inflammation in Rats
IS  - 1
VL  - 22
EP  - 7
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1464
ER  - 

@article{
author = "Kataranovski, Milena and Janković, Srđa J and Kataranovski, Dragan S. and Stošić, Jelena and Bogojević, Desanka",
year = "2009",
abstract = "To examine the presence of gender differences in pro-inflammatory potential of cadmium in rats by comparing systemic inflammatory response to acute cadmium intoxication in animals of the two sexes. Methods Basic aspects of this response were evaluated, including plasma levels of inflammatory cytokines tumor necrosis factor (TNF) and interleukin-6 (IL-6) and of major rat acute phase protein alpha 2-macroglobulin (alpha2-M), as Soluble indicators of inflammation, and the number and activity of peripheral blood leukocytes, as cellular indicators of inflammation. Results Differential increases of IL-6 and alpha 2-M (higher in males than in females) in peripheral blood cell counts and types (leukocytosis and shift in the ratio of granulocytes to lymphocytes more pronounced in males vs females) and in levels of neutrophil priming (higher in males vs females) were noted. Conclusion The data document a more intense inflammatory response to cadmium administration in males. The sex differences in inflammatory effects of cadmium might be taken into consideration in studying the toxicity of this heavy metal.",
journal = "Biomedical and Environmental Sciences",
title = "Gender Differences in Acute Cadmium-Induced Systemic Inflammation in Rats",
number = "1",
volume = "22",
pages = "7",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1464"
}

Kataranovski, M., Janković, S. J., Kataranovski, D. S., Stošić, J.,& Bogojević, D.. (2009). Gender Differences in Acute Cadmium-Induced Systemic Inflammation in Rats. in Biomedical and Environmental Sciences, 22(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1464

Kataranovski M, Janković SJ, Kataranovski DS, Stošić J, Bogojević D. Gender Differences in Acute Cadmium-Induced Systemic Inflammation in Rats. in Biomedical and Environmental Sciences. 2009;22(1):null-7.
https://hdl.handle.net/21.15107/rcub_ibiss_1464 .

Kataranovski, Milena, Janković, Srđa J, Kataranovski, Dragan S., Stošić, Jelena, Bogojević, Desanka, "Gender Differences in Acute Cadmium-Induced Systemic Inflammation in Rats" in Biomedical and Environmental Sciences, 22, no. 1 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1464 .