TY  - JOUR
AU  - Radenović, Lidija
AU  - Selaković, Vesna
AU  - Olivan, Sara
AU  - Calvo, Ana Cristina
AU  - Rando, Amaya
AU  - Petković, Branka
AU  - Osta, Rosario
PY  - 2014
UR  - https://www.sciencedirect.com/science/article/abs/pii/S0361923013001871
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3787
AB  - The tetanus toxin C (TTC) fragment capacity of being transported in a retrograde way through motoneurons and its nontoxic nature opens the door to a new promising therapeutic strategy for neurodegenerative diseases. In this study, the TTC effect was tested for the first time in animal model of global cerebral ischemia induced by 10-min occlusion of both common carotid arteries. The aim was to evaluate the effect of TTC gene therapy treatment on the development and expression of global cerebral ischemia/reperfusion-induced oxidative stress and motor hyperactivity in Mongolian gerbils. Several oxidative stress and motor behavioral parameters were investigated between 2 h and 14 days after reperfusion. Neuroprotective efficiency of TTC was observed in the forebrain cortex, striatum, hippocampus, and cerebellum at the level of each examined oxidative stress parameter (nitric oxide level, superoxide production, superoxide dismutase activity, and index of lipid peroxidation). Additionally, TTC significantly decreased ischemia-induced motor hyperactivity based on tested parameters (locomotion, stereotypy, and rotations). As judged by biochemical as well as behavioral data, treatment with TTC for the first time showed neuroprotective efficiency by reduction of ischemia-induced oxidative stress and motor hyperactivity and can be a promising strategy for ischemia-induced neuronal damage treatment.
PB  - Pergamon-Elsevier Science Ltd
T2  - Brain Research Bulletin
T1  - Neuroprotective efficiency of tetanus toxin C fragment in model of global cerebral ischemia in Mongolian gerbils
VL  - 101
DO  - 10.1016/j.brainresbull.2013.11.006
SP  - 37
EP  - 44
ER  - 

@article{
author = "Radenović, Lidija and Selaković, Vesna and Olivan, Sara and Calvo, Ana Cristina and Rando, Amaya and Petković, Branka and Osta, Rosario",
year = "2014",
abstract = "The tetanus toxin C (TTC) fragment capacity of being transported in a retrograde way through motoneurons and its nontoxic nature opens the door to a new promising therapeutic strategy for neurodegenerative diseases. In this study, the TTC effect was tested for the first time in animal model of global cerebral ischemia induced by 10-min occlusion of both common carotid arteries. The aim was to evaluate the effect of TTC gene therapy treatment on the development and expression of global cerebral ischemia/reperfusion-induced oxidative stress and motor hyperactivity in Mongolian gerbils. Several oxidative stress and motor behavioral parameters were investigated between 2 h and 14 days after reperfusion. Neuroprotective efficiency of TTC was observed in the forebrain cortex, striatum, hippocampus, and cerebellum at the level of each examined oxidative stress parameter (nitric oxide level, superoxide production, superoxide dismutase activity, and index of lipid peroxidation). Additionally, TTC significantly decreased ischemia-induced motor hyperactivity based on tested parameters (locomotion, stereotypy, and rotations). As judged by biochemical as well as behavioral data, treatment with TTC for the first time showed neuroprotective efficiency by reduction of ischemia-induced oxidative stress and motor hyperactivity and can be a promising strategy for ischemia-induced neuronal damage treatment.",
publisher = "Pergamon-Elsevier Science Ltd",
journal = "Brain Research Bulletin",
title = "Neuroprotective efficiency of tetanus toxin C fragment in model of global cerebral ischemia in Mongolian gerbils",
volume = "101",
doi = "10.1016/j.brainresbull.2013.11.006",
pages = "37-44"
}

Radenović, L., Selaković, V., Olivan, S., Calvo, A. C., Rando, A., Petković, B.,& Osta, R.. (2014). Neuroprotective efficiency of tetanus toxin C fragment in model of global cerebral ischemia in Mongolian gerbils. in Brain Research Bulletin
Pergamon-Elsevier Science Ltd., 101, 37-44.
https://doi.org/10.1016/j.brainresbull.2013.11.006

Radenović L, Selaković V, Olivan S, Calvo AC, Rando A, Petković B, Osta R. Neuroprotective efficiency of tetanus toxin C fragment in model of global cerebral ischemia in Mongolian gerbils. in Brain Research Bulletin. 2014;101:37-44.
doi:10.1016/j.brainresbull.2013.11.006 .

Radenović, Lidija, Selaković, Vesna, Olivan, Sara, Calvo, Ana Cristina, Rando, Amaya, Petković, Branka, Osta, Rosario, "Neuroprotective efficiency of tetanus toxin C fragment in model of global cerebral ischemia in Mongolian gerbils" in Brain Research Bulletin, 101 (2014):37-44,
https://doi.org/10.1016/j.brainresbull.2013.11.006 . .

TY  - JOUR
AU  - Rauš Balind, Snežana
AU  - Selaković, Vesna
AU  - Radenović, Lidija
AU  - Prolić, Zlatko
AU  - Petković, Branka
PY  - 2014
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0088921
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3788
AB  - Magnetic field as ecological factor has influence on all living beings. The aim of this study was to determine if extremely low frequency magnetic field (ELF-MF, 50 Hz, 0.5 mT) affects oxidative stress in the brain of gerbils submitted to 10-min global cerebral ischemia. After occlusion of both carotid arteries, 3-month-old gerbils were continuously exposed to ELF-MF for 7 days. Nitric oxide and superoxide anion production, superoxide dismutase activity and index of lipid peroxidation were examined in the forebrain cortex, striatum and hippocampus on the 7th (immediate effect of ELF-MF) and 14th day after reperfusion (delayed effect of ELF-MF). Ischemia per se increased oxidative stress in the brain on the 7th and 14th day after reperfusion. ELF-MF also increased oxidative stress, but to a greater extent than ischemia, only immediately after cessation of exposure. Ischemic gerbils exposed to ELF-MF had increased oxidative stress parameters on the 7th day after reperfusion, but to a lesser extent than ischemic or ELF-MF-exposed animals. On the 14th day after reperfusion, oxidative stress parameters in the brain of these gerbils were mostly at the control levels. Applied ELF-MF decreases oxidative stress induced by global cerebral ischemia and thereby reduces possible negative consequences which free radical species could have in the brain. The results presented here indicate a beneficial effect of ELF-MF (50 Hz, 0.5 mT) in the model of global cerebral ischemia.
PB  - Public Library of Science
T2  - PLoS One
T1  - Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia
IS  - 2
IS  - 2
VL  - 9
DO  - 10.1371/journal.pone.0088921
SP  - e88921
ER  - 

@article{
author = "Rauš Balind, Snežana and Selaković, Vesna and Radenović, Lidija and Prolić, Zlatko and Petković, Branka",
year = "2014",
abstract = "Magnetic field as ecological factor has influence on all living beings. The aim of this study was to determine if extremely low frequency magnetic field (ELF-MF, 50 Hz, 0.5 mT) affects oxidative stress in the brain of gerbils submitted to 10-min global cerebral ischemia. After occlusion of both carotid arteries, 3-month-old gerbils were continuously exposed to ELF-MF for 7 days. Nitric oxide and superoxide anion production, superoxide dismutase activity and index of lipid peroxidation were examined in the forebrain cortex, striatum and hippocampus on the 7th (immediate effect of ELF-MF) and 14th day after reperfusion (delayed effect of ELF-MF). Ischemia per se increased oxidative stress in the brain on the 7th and 14th day after reperfusion. ELF-MF also increased oxidative stress, but to a greater extent than ischemia, only immediately after cessation of exposure. Ischemic gerbils exposed to ELF-MF had increased oxidative stress parameters on the 7th day after reperfusion, but to a lesser extent than ischemic or ELF-MF-exposed animals. On the 14th day after reperfusion, oxidative stress parameters in the brain of these gerbils were mostly at the control levels. Applied ELF-MF decreases oxidative stress induced by global cerebral ischemia and thereby reduces possible negative consequences which free radical species could have in the brain. The results presented here indicate a beneficial effect of ELF-MF (50 Hz, 0.5 mT) in the model of global cerebral ischemia.",
publisher = "Public Library of Science",
journal = "PLoS One",
title = "Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia",
number = "2, 2",
volume = "9",
doi = "10.1371/journal.pone.0088921",
pages = "e88921"
}

Rauš Balind, S., Selaković, V., Radenović, L., Prolić, Z.,& Petković, B.. (2014). Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia. in PLoS One
Public Library of Science., 9(2), e88921.
https://doi.org/10.1371/journal.pone.0088921

Rauš Balind S, Selaković V, Radenović L, Prolić Z, Petković B. Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia. in PLoS One. 2014;9(2):e88921.
doi:10.1371/journal.pone.0088921 .

Rauš Balind, Snežana, Selaković, Vesna, Radenović, Lidija, Prolić, Zlatko, Petković, Branka, "Extremely Low Frequency Magnetic Field (50 Hz, 0.5 mT) Reduces Oxidative Stress in the Brain of Gerbils Submitted to Global Cerebral Ischemia" in PLoS One, 9, no. 2 (2014):e88921,
https://doi.org/10.1371/journal.pone.0088921 . .

TY  - JOUR
AU  - Selaković, Vesna
AU  - Rauš Balind, Snežana
AU  - Radenović, Lidija
AU  - Prolić, Zlatko
AU  - Petković, Branka
PY  - 2013
UR  - https://link.springer.com/article/10.1007/s12013-012-9498-z
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3785
AB  - The aim of study was to investigate the effects of extremely low frequency magnetic field (ELF-MF; 50 Hz; 0.1, 0.25 and 0.5 mT) on oxidative stress in the brain of 3- (adult) and 10-month-old (middle-aged) gerbils. Nitric oxide (NO) level, superoxide (O2-) production, superoxide dismutase (SOD) activity, and index of lipid peroxidation (ILP) were measured in the forebrain cortex, striatum, hippocampus, and cerebellum immediately and 3 days after cessation of 7-day exposure. In all gerbils, ELF-MF significantly increased oxidative stress in all tested brain regions. This effect was correlated with the value of magnetic induction and was higher in middle-aged gerbils. Three days after cessation of exposure, the values of examined parameters were closer to control levels. In adult gerbils, the effect of ELF-MF of 0.1 mT on NO level, O2- production and SOD activity was almost fully disappeared, and ILP was at the control level regardless of the value of magnetic induction. In middle-aged gerbils, the effect of ELF-MF was still present but to a lesser degree than those observed immediately after cessation of exposure. These findings pointed out the ability of ELF-MF to induce age- and magnetic induction-dependent modification of oxidative stress in the brain.
PB  - Humana Press Inc.
T2  - Cell Biochemistry and Biophysics
T1  - Age-Dependent Effects of ELF-MF on Oxidative Stress in the Brain of Mongolian Gerbils
IS  - 3
VL  - 66
DO  - 10.1007/s12013-012-9498-z
SP  - 513
EP  - 521
ER  - 

@article{
author = "Selaković, Vesna and Rauš Balind, Snežana and Radenović, Lidija and Prolić, Zlatko and Petković, Branka",
year = "2013",
abstract = "The aim of study was to investigate the effects of extremely low frequency magnetic field (ELF-MF; 50 Hz; 0.1, 0.25 and 0.5 mT) on oxidative stress in the brain of 3- (adult) and 10-month-old (middle-aged) gerbils. Nitric oxide (NO) level, superoxide (O2-) production, superoxide dismutase (SOD) activity, and index of lipid peroxidation (ILP) were measured in the forebrain cortex, striatum, hippocampus, and cerebellum immediately and 3 days after cessation of 7-day exposure. In all gerbils, ELF-MF significantly increased oxidative stress in all tested brain regions. This effect was correlated with the value of magnetic induction and was higher in middle-aged gerbils. Three days after cessation of exposure, the values of examined parameters were closer to control levels. In adult gerbils, the effect of ELF-MF of 0.1 mT on NO level, O2- production and SOD activity was almost fully disappeared, and ILP was at the control level regardless of the value of magnetic induction. In middle-aged gerbils, the effect of ELF-MF was still present but to a lesser degree than those observed immediately after cessation of exposure. These findings pointed out the ability of ELF-MF to induce age- and magnetic induction-dependent modification of oxidative stress in the brain.",
publisher = "Humana Press Inc.",
journal = "Cell Biochemistry and Biophysics",
title = "Age-Dependent Effects of ELF-MF on Oxidative Stress in the Brain of Mongolian Gerbils",
number = "3",
volume = "66",
doi = "10.1007/s12013-012-9498-z",
pages = "513-521"
}

Selaković, V., Rauš Balind, S., Radenović, L., Prolić, Z.,& Petković, B.. (2013). Age-Dependent Effects of ELF-MF on Oxidative Stress in the Brain of Mongolian Gerbils. in Cell Biochemistry and Biophysics
Humana Press Inc.., 66(3), 513-521.
https://doi.org/10.1007/s12013-012-9498-z

Selaković V, Rauš Balind S, Radenović L, Prolić Z, Petković B. Age-Dependent Effects of ELF-MF on Oxidative Stress in the Brain of Mongolian Gerbils. in Cell Biochemistry and Biophysics. 2013;66(3):513-521.
doi:10.1007/s12013-012-9498-z .

Selaković, Vesna, Rauš Balind, Snežana, Radenović, Lidija, Prolić, Zlatko, Petković, Branka, "Age-Dependent Effects of ELF-MF on Oxidative Stress in the Brain of Mongolian Gerbils" in Cell Biochemistry and Biophysics, 66, no. 3 (2013):513-521,
https://doi.org/10.1007/s12013-012-9498-z . .

TY  - JOUR
AU  - Rauš Balind, Snežana
AU  - Selaković, Vesna
AU  - Manojlović-Stojanoski, Milica
AU  - Radenović, Lidija
AU  - Prolić, Zlatko
AU  - Petković, Branka
PY  - 2013
UR  - https://link.springer.com/article/10.1007/s12640-012-9333-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3783
AB  - The purpose of this study was to determine whether exposure to an extremely low-frequency magnetic field (ELF-MF, 50 Hz) affects the outcome of postischemic damage in the hippocampus of Mongolian gerbils. After 10-min bilateral carotid occlusion, the gerbils were continuously exposed to ELF-MF (average magnetic induction at the center of the cage was 0.5 mT) for 7 days. The impact of ELF-MF was estimated immediately (the 7th day after reperfusion) and 7 days after cessation of exposure (the 14th day after reperfusion) compared with ischemic gerbils without ELF-MF exposure. Applying stereological methods, histological evaluation of changes in the hippocampus was done for determining its volume, volume densities of degenerating neurons and astrocytes, as well as the number of microglial cells per unit area. ELF-MF per se did not induce any morphological changes, while 10-min global cerebral ischemia led to neuronal death, especially in CA1 region of the hippocampus, as expected. Ischemic gerbils exposed to ELF-MF had significantly a lower degree of cell loss in the examined structure and greater responses of astrocytes and microglial cells than postischemic gerbils without exposure on the seventh day after reperfusion (immediate effect of ELF-MF). Similar response was observed on the 14th day after reperfusion (delayed effect of ELF-MF); however, differences in measured parameters were low and insignificant. Applied ELF-MF has possible neuroprotective function in the hippocampus, as the most sensitive brain structure in the model of global cerebral ischemia, through reduction of neuronal death and activation of astrocytes and microglial cells.
PB  - Springer
T2  - Neurotoxicity Research
T1  - Response of Hippocampal Neurons and Glial Cells to Alternating Magnetic Field in Gerbils Submitted to Global Cerebral Ischemia
IS  - 1
VL  - 23
DO  - 10.1007/s12640-012-9333-8
SP  - 79
EP  - 91
ER  - 

@article{
author = "Rauš Balind, Snežana and Selaković, Vesna and Manojlović-Stojanoski, Milica and Radenović, Lidija and Prolić, Zlatko and Petković, Branka",
year = "2013",
abstract = "The purpose of this study was to determine whether exposure to an extremely low-frequency magnetic field (ELF-MF, 50 Hz) affects the outcome of postischemic damage in the hippocampus of Mongolian gerbils. After 10-min bilateral carotid occlusion, the gerbils were continuously exposed to ELF-MF (average magnetic induction at the center of the cage was 0.5 mT) for 7 days. The impact of ELF-MF was estimated immediately (the 7th day after reperfusion) and 7 days after cessation of exposure (the 14th day after reperfusion) compared with ischemic gerbils without ELF-MF exposure. Applying stereological methods, histological evaluation of changes in the hippocampus was done for determining its volume, volume densities of degenerating neurons and astrocytes, as well as the number of microglial cells per unit area. ELF-MF per se did not induce any morphological changes, while 10-min global cerebral ischemia led to neuronal death, especially in CA1 region of the hippocampus, as expected. Ischemic gerbils exposed to ELF-MF had significantly a lower degree of cell loss in the examined structure and greater responses of astrocytes and microglial cells than postischemic gerbils without exposure on the seventh day after reperfusion (immediate effect of ELF-MF). Similar response was observed on the 14th day after reperfusion (delayed effect of ELF-MF); however, differences in measured parameters were low and insignificant. Applied ELF-MF has possible neuroprotective function in the hippocampus, as the most sensitive brain structure in the model of global cerebral ischemia, through reduction of neuronal death and activation of astrocytes and microglial cells.",
publisher = "Springer",
journal = "Neurotoxicity Research",
title = "Response of Hippocampal Neurons and Glial Cells to Alternating Magnetic Field in Gerbils Submitted to Global Cerebral Ischemia",
number = "1",
volume = "23",
doi = "10.1007/s12640-012-9333-8",
pages = "79-91"
}

Rauš Balind, S., Selaković, V., Manojlović-Stojanoski, M., Radenović, L., Prolić, Z.,& Petković, B.. (2013). Response of Hippocampal Neurons and Glial Cells to Alternating Magnetic Field in Gerbils Submitted to Global Cerebral Ischemia. in Neurotoxicity Research
Springer., 23(1), 79-91.
https://doi.org/10.1007/s12640-012-9333-8

Rauš Balind S, Selaković V, Manojlović-Stojanoski M, Radenović L, Prolić Z, Petković B. Response of Hippocampal Neurons and Glial Cells to Alternating Magnetic Field in Gerbils Submitted to Global Cerebral Ischemia. in Neurotoxicity Research. 2013;23(1):79-91.
doi:10.1007/s12640-012-9333-8 .

Rauš Balind, Snežana, Selaković, Vesna, Manojlović-Stojanoski, Milica, Radenović, Lidija, Prolić, Zlatko, Petković, Branka, "Response of Hippocampal Neurons and Glial Cells to Alternating Magnetic Field in Gerbils Submitted to Global Cerebral Ischemia" in Neurotoxicity Research, 23, no. 1 (2013):79-91,
https://doi.org/10.1007/s12640-012-9333-8 . .

TY  - JOUR
AU  - Rauš Balind, Snežana
AU  - Selaković, Vesna
AU  - Radenović, Lidija
AU  - Prolić, Zlatko
AU  - Petković, Branka
PY  - 2012
UR  - https://www.sciencedirect.com/science/article/pii/S0166432811007790?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3801
AB  - The purpose of this study was to evaluate behavioural effects of an extremely low frequency magnetic field (ELF-MF) in 3-month-old Mongolian gerbils submitted to global cerebral ischemia. After 10-min occlusion of both common carotid arteries, the gerbils were placed in the vicinity of an electromagnet and continuously exposed to ELF-MF (50 Hz, 0.5 mT) for 7 days. Their behaviour (locomotion, stereotypy, rotations, and immobility) was monitored on days 1, 2, 4, 7, and 14 after reperfusion for 60 min in the open field. It was shown that the 10-min global cerebral ischemia per se induced a significant motor activity increase (locomotion, stereotypy and rotations), and consequently immobility decrease until day 4 after reperfusion, compared to control gerbils. Exposure to ELF-MF inhibited development of ischemia-induced motor hyperactivity during the whole period of registration, but significantly in the first 2 days after reperfusion, when the postischemic hyperactivity was most evident. Motor activity of these gerbils was still significantly increased compared to control ones, but only on day 1 after reperfusion. Our results revealed that the applied ELF-MF (50 Hz, 0.5 mT) decreased motor hyperactivity induced by the 10-min global cerebral ischemia, via modulation of the processes that underlie this behavioural response.
PB  - Elsevier Science BV
T2  - Behavioural Brain Research
T1  - Extremely low frequency magnetic field induced changes in motor behaviour of gerbils submitted to global cerebral ischemia
IS  - 2
VL  - 228
DO  - 10.1016/j.bbr.2011.10.046
SP  - 241
EP  - 246
ER  - 

@article{
author = "Rauš Balind, Snežana and Selaković, Vesna and Radenović, Lidija and Prolić, Zlatko and Petković, Branka",
year = "2012",
abstract = "The purpose of this study was to evaluate behavioural effects of an extremely low frequency magnetic field (ELF-MF) in 3-month-old Mongolian gerbils submitted to global cerebral ischemia. After 10-min occlusion of both common carotid arteries, the gerbils were placed in the vicinity of an electromagnet and continuously exposed to ELF-MF (50 Hz, 0.5 mT) for 7 days. Their behaviour (locomotion, stereotypy, rotations, and immobility) was monitored on days 1, 2, 4, 7, and 14 after reperfusion for 60 min in the open field. It was shown that the 10-min global cerebral ischemia per se induced a significant motor activity increase (locomotion, stereotypy and rotations), and consequently immobility decrease until day 4 after reperfusion, compared to control gerbils. Exposure to ELF-MF inhibited development of ischemia-induced motor hyperactivity during the whole period of registration, but significantly in the first 2 days after reperfusion, when the postischemic hyperactivity was most evident. Motor activity of these gerbils was still significantly increased compared to control ones, but only on day 1 after reperfusion. Our results revealed that the applied ELF-MF (50 Hz, 0.5 mT) decreased motor hyperactivity induced by the 10-min global cerebral ischemia, via modulation of the processes that underlie this behavioural response.",
publisher = "Elsevier Science BV",
journal = "Behavioural Brain Research",
title = "Extremely low frequency magnetic field induced changes in motor behaviour of gerbils submitted to global cerebral ischemia",
number = "2",
volume = "228",
doi = "10.1016/j.bbr.2011.10.046",
pages = "241-246"
}

Rauš Balind, S., Selaković, V., Radenović, L., Prolić, Z.,& Petković, B.. (2012). Extremely low frequency magnetic field induced changes in motor behaviour of gerbils submitted to global cerebral ischemia. in Behavioural Brain Research
Elsevier Science BV., 228(2), 241-246.
https://doi.org/10.1016/j.bbr.2011.10.046

Rauš Balind S, Selaković V, Radenović L, Prolić Z, Petković B. Extremely low frequency magnetic field induced changes in motor behaviour of gerbils submitted to global cerebral ischemia. in Behavioural Brain Research. 2012;228(2):241-246.
doi:10.1016/j.bbr.2011.10.046 .

Rauš Balind, Snežana, Selaković, Vesna, Radenović, Lidija, Prolić, Zlatko, Petković, Branka, "Extremely low frequency magnetic field induced changes in motor behaviour of gerbils submitted to global cerebral ischemia" in Behavioural Brain Research, 228, no. 2 (2012):241-246,
https://doi.org/10.1016/j.bbr.2011.10.046 . .

TY  - JOUR
AU  - Radenović, Lidija
AU  - Selaković, Vesna
AU  - Petković, Branka
AU  - Anđus, Pavle R.
PY  - 2011
UR  - https://akjournals.com/view/journals/036/98/1/article-p32.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3799
AB  - The purpose of this study was to investigate neuroprotective efficiency of N-methyl D-aspartate (NMDA) receptor (NMDAR) blockade on the neuronal damage in the less studied and allegedly less affected CA3 hippocampus and striatum in the Mongolian gerbil model of global cerebral ischemia. The common carotid arteries of gerbils were occluded for 5, 10 or 15 minutes. Gerbils were given a low dose of non-competitive NMDA antagonist (MK-801, 3 mg/kg i.p.) or saline immediately after the occlusion in normothermic conditions. Neuronal damage was examined on 4(th), 14(th) and 28(th) day after reperfusion. The effect of NMDAR blockade was followed in vivo by monitoring the neurological status of whole animals or at the cellular level by standard light- and confocal-microscopy on brain slices. Increased duration of cerebral ischemia resulted in a progressive loss of striatal and CA3 hippocampal neurons. The most beneficial NMDAR blockade effect was observed when the neuronal damage was most severe - on the 28(th) day after 15-min ischemia. As judged by morphological and neurological data, the effect of ischemia is also apparent in the presumed less vulnerable regions (CA3 and striatum) which are functionally important in stroke plasticity. So, NMDAR blockade in normothermic conditions showed neuroprotective efficiency.
PB  - Akadémiai Kiadó Zrt
T2  - Acta Physiologica Hungarica
T1  - Neuroprotective efficiency of NMDA receptor blockade in the striatum and CA3 hippocampus after various durations of cerebral ischemia in gerbils
IS  - 1
VL  - 98
DO  - 10.1556/APhysiol.98.2011.1.5
SP  - 32
EP  - 44
ER  - 

@article{
author = "Radenović, Lidija and Selaković, Vesna and Petković, Branka and Anđus, Pavle R.",
year = "2011",
abstract = "The purpose of this study was to investigate neuroprotective efficiency of N-methyl D-aspartate (NMDA) receptor (NMDAR) blockade on the neuronal damage in the less studied and allegedly less affected CA3 hippocampus and striatum in the Mongolian gerbil model of global cerebral ischemia. The common carotid arteries of gerbils were occluded for 5, 10 or 15 minutes. Gerbils were given a low dose of non-competitive NMDA antagonist (MK-801, 3 mg/kg i.p.) or saline immediately after the occlusion in normothermic conditions. Neuronal damage was examined on 4(th), 14(th) and 28(th) day after reperfusion. The effect of NMDAR blockade was followed in vivo by monitoring the neurological status of whole animals or at the cellular level by standard light- and confocal-microscopy on brain slices. Increased duration of cerebral ischemia resulted in a progressive loss of striatal and CA3 hippocampal neurons. The most beneficial NMDAR blockade effect was observed when the neuronal damage was most severe - on the 28(th) day after 15-min ischemia. As judged by morphological and neurological data, the effect of ischemia is also apparent in the presumed less vulnerable regions (CA3 and striatum) which are functionally important in stroke plasticity. So, NMDAR blockade in normothermic conditions showed neuroprotective efficiency.",
publisher = "Akadémiai Kiadó Zrt",
journal = "Acta Physiologica Hungarica",
title = "Neuroprotective efficiency of NMDA receptor blockade in the striatum and CA3 hippocampus after various durations of cerebral ischemia in gerbils",
number = "1",
volume = "98",
doi = "10.1556/APhysiol.98.2011.1.5",
pages = "32-44"
}

Radenović, L., Selaković, V., Petković, B.,& Anđus, P. R.. (2011). Neuroprotective efficiency of NMDA receptor blockade in the striatum and CA3 hippocampus after various durations of cerebral ischemia in gerbils. in Acta Physiologica Hungarica
Akadémiai Kiadó Zrt., 98(1), 32-44.
https://doi.org/10.1556/APhysiol.98.2011.1.5

Radenović L, Selaković V, Petković B, Anđus PR. Neuroprotective efficiency of NMDA receptor blockade in the striatum and CA3 hippocampus after various durations of cerebral ischemia in gerbils. in Acta Physiologica Hungarica. 2011;98(1):32-44.
doi:10.1556/APhysiol.98.2011.1.5 .

Radenović, Lidija, Selaković, Vesna, Petković, Branka, Anđus, Pavle R., "Neuroprotective efficiency of NMDA receptor blockade in the striatum and CA3 hippocampus after various durations of cerebral ischemia in gerbils" in Acta Physiologica Hungarica, 98, no. 1 (2011):32-44,
https://doi.org/10.1556/APhysiol.98.2011.1.5 . .

TY  - JOUR
AU  - Petković, Branka
AU  - Selaković, Vesna
AU  - Radenović, Lidija
PY  - 2008
UR  - https://www.sciencedirect.com/science/article/pii/S0166432808003252?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3795
AB  - The purpose of this study was to investigate the temporal pattern of NMDA receptors antagonist-MK-801 on motor behaviour parameters in gerbils submitted to different duration of global cerebral ischemia. The common carotid arteries of gerbils were occluded for 5, 10 or 15 min. Gerbils were given MK-801 (3 mg/kg i.p.) or saline immediately after the occlusion in normothermic conditions prior to testing. Motor activity was registered 1, 2, 4, 7,14,21 and 28 days after reperfusion during 60 min by open field test. At the same time, the effect of NMDA receptor blockade was followed in vivo by monitoring the neurological status of whole animals or at the cellular level by standard light and confocal microscopy on brain slices. Postischemic gerbils quickly developed hypermotor response with the most intensity in animals submitted to 15 min ischemia. MK-801 administrated immediately after ischemia significantly decreased this hyperactivity. In all ischemic-treated animals, behavioural suppression by MK-801 was observed already 1 day after occlusion and was lasting as far as observed ischemia-dependent hypermotor responses. Beneficial effect of MK-801 was also confirmed by morphological and neurological status data. These findings suggest that sustained ischemia-induced hyperactivity is related to abnormalities in NMDA glutamatergic function, as well as its manifestation could be completely abolished by NMDA receptor blockade immediately after ischemic insult.
PB  - Elsevier Science BV
T2  - Behavioural Brain Research
T1  - Temporal patterns of motor behavioural improvements by MK-801 in Mongolian gerbils submitted to different duration of global cerebral ischemia
IS  - 1
VL  - 194
DO  - 10.1016/j.bbr.2008.06.031
SP  - 72
EP  - 78
ER  - 

@article{
author = "Petković, Branka and Selaković, Vesna and Radenović, Lidija",
year = "2008",
abstract = "The purpose of this study was to investigate the temporal pattern of NMDA receptors antagonist-MK-801 on motor behaviour parameters in gerbils submitted to different duration of global cerebral ischemia. The common carotid arteries of gerbils were occluded for 5, 10 or 15 min. Gerbils were given MK-801 (3 mg/kg i.p.) or saline immediately after the occlusion in normothermic conditions prior to testing. Motor activity was registered 1, 2, 4, 7,14,21 and 28 days after reperfusion during 60 min by open field test. At the same time, the effect of NMDA receptor blockade was followed in vivo by monitoring the neurological status of whole animals or at the cellular level by standard light and confocal microscopy on brain slices. Postischemic gerbils quickly developed hypermotor response with the most intensity in animals submitted to 15 min ischemia. MK-801 administrated immediately after ischemia significantly decreased this hyperactivity. In all ischemic-treated animals, behavioural suppression by MK-801 was observed already 1 day after occlusion and was lasting as far as observed ischemia-dependent hypermotor responses. Beneficial effect of MK-801 was also confirmed by morphological and neurological status data. These findings suggest that sustained ischemia-induced hyperactivity is related to abnormalities in NMDA glutamatergic function, as well as its manifestation could be completely abolished by NMDA receptor blockade immediately after ischemic insult.",
publisher = "Elsevier Science BV",
journal = "Behavioural Brain Research",
title = "Temporal patterns of motor behavioural improvements by MK-801 in Mongolian gerbils submitted to different duration of global cerebral ischemia",
number = "1",
volume = "194",
doi = "10.1016/j.bbr.2008.06.031",
pages = "72-78"
}

Petković, B., Selaković, V.,& Radenović, L.. (2008). Temporal patterns of motor behavioural improvements by MK-801 in Mongolian gerbils submitted to different duration of global cerebral ischemia. in Behavioural Brain Research
Elsevier Science BV., 194(1), 72-78.
https://doi.org/10.1016/j.bbr.2008.06.031

Petković B, Selaković V, Radenović L. Temporal patterns of motor behavioural improvements by MK-801 in Mongolian gerbils submitted to different duration of global cerebral ischemia. in Behavioural Brain Research. 2008;194(1):72-78.
doi:10.1016/j.bbr.2008.06.031 .

Petković, Branka, Selaković, Vesna, Radenović, Lidija, "Temporal patterns of motor behavioural improvements by MK-801 in Mongolian gerbils submitted to different duration of global cerebral ischemia" in Behavioural Brain Research, 194, no. 1 (2008):72-78,
https://doi.org/10.1016/j.bbr.2008.06.031 . .

TY  - JOUR
AU  - Radenović, Lidija
AU  - Todorović, Dajana
AU  - Selaković, Vesna
AU  - Petković, Branka
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/146
AB  - Stimulation of glutamate receptors induces neuronal nitric oxide (NO) release, which in turn modulates glutamate transmission. The involvement of ionotropic glutamate NMDA and AMPA/kainate receptors in induction of NO production in the rat brain was examined after injection of kainate, a non-NMDA receptor agonist; kainate plus 6-cyano- 7-nitroquinoxaline-2,3-dione (CNQX), a selective AMPA/kainate receptor antagonist; or kainate plus 2-amino-5-phosphonopentanoic acid (APV), a selective NMDA receptor antagonist. Competitive glutamate receptor antagonists were injected with kainate unilaterally into the CA3 region of the rat hippocampus. The accumulation of nitrite, the stable metabolite of NO, was measured by the Griess reaction at different times (5 min, 15 min, 2 h, 48 h, and 7 days) in hippocampus, forebrain cortex, striatum, and cerebellum homogenates. The used glutamate antagonists APV and CNQX both provided sufficient neuroprotection in the sense of reducing nitrite concentrations, but with different mechanisms and time dynamics. Our findings suggest that NMDA and AMPA/kainate receptors are differentially involved in nitric oxide production.
AB  - Stimulacija glutamatskih receptora dovodi do stvaranja azot oksida (NO) u neuronima mozga što dovodi do modulacije glutamatske neurotransmisije. Ispitivana je uloga glutamatskih NMDA i AMPA/kainatnih receptora u stvaranju NO u mozgu pacova posle intracerebralne aplikacija kainata, agonista AMPA/kainatnih receptora, kainata sa 6-ciano-7- nitrokinoksalin-2,3-dionom (CNQX), selektivnim antagonistom AMPA/kainatnih receptora ili kainata sa 2-amino-5-fosfonopentanoiskom kiselinom (APV) selektivnim antagonistom NMDA receptora. Antagonisti glutamata su aplicirani unilateralno u selektivno osetljiv CA3 region hipokampusa. Stvaranje NO je praćeno preko akumulacije nitrita, stabilnih metabolita NO, Griess-ovom metodom. Merenja su vršena u hipokampusu, korteksu, stiatumu i cerebelumu mozga pacova 5 min, 15 min, 2 h, 48 h i 7 dana nakon aplikacije. U svim praćenim moždanim strukturama neuroprotektivno je delovala primena CNQX i APV u smislu smanjenja produkcije NO, ali sa očiglednom razlikom u mehanizmu dejstva i vremenskoj dinamici. Rezultati našeg istraživanja dokazuju da su glutamatski NMDA i AMPA/kainatni receptori različito uključeni u proces produkcije NO.
T2  - Archives of Biological Sciences
T1  - Efekat antagonista glutamata na stvaranje azot oksida u mozgu pacova
T1  - Effect of glutamate antagonists on nitric oxide production in rat brain following intrahippocampal injection
IS  - 1
VL  - 59
SP  - 29
EP  - 36
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_146
ER  - 

@article{
author = "Radenović, Lidija and Todorović, Dajana and Selaković, Vesna and Petković, Branka",
year = "2007, 2007",
abstract = "Stimulation of glutamate receptors induces neuronal nitric oxide (NO) release, which in turn modulates glutamate transmission. The involvement of ionotropic glutamate NMDA and AMPA/kainate receptors in induction of NO production in the rat brain was examined after injection of kainate, a non-NMDA receptor agonist; kainate plus 6-cyano- 7-nitroquinoxaline-2,3-dione (CNQX), a selective AMPA/kainate receptor antagonist; or kainate plus 2-amino-5-phosphonopentanoic acid (APV), a selective NMDA receptor antagonist. Competitive glutamate receptor antagonists were injected with kainate unilaterally into the CA3 region of the rat hippocampus. The accumulation of nitrite, the stable metabolite of NO, was measured by the Griess reaction at different times (5 min, 15 min, 2 h, 48 h, and 7 days) in hippocampus, forebrain cortex, striatum, and cerebellum homogenates. The used glutamate antagonists APV and CNQX both provided sufficient neuroprotection in the sense of reducing nitrite concentrations, but with different mechanisms and time dynamics. Our findings suggest that NMDA and AMPA/kainate receptors are differentially involved in nitric oxide production., Stimulacija glutamatskih receptora dovodi do stvaranja azot oksida (NO) u neuronima mozga što dovodi do modulacije glutamatske neurotransmisije. Ispitivana je uloga glutamatskih NMDA i AMPA/kainatnih receptora u stvaranju NO u mozgu pacova posle intracerebralne aplikacija kainata, agonista AMPA/kainatnih receptora, kainata sa 6-ciano-7- nitrokinoksalin-2,3-dionom (CNQX), selektivnim antagonistom AMPA/kainatnih receptora ili kainata sa 2-amino-5-fosfonopentanoiskom kiselinom (APV) selektivnim antagonistom NMDA receptora. Antagonisti glutamata su aplicirani unilateralno u selektivno osetljiv CA3 region hipokampusa. Stvaranje NO je praćeno preko akumulacije nitrita, stabilnih metabolita NO, Griess-ovom metodom. Merenja su vršena u hipokampusu, korteksu, stiatumu i cerebelumu mozga pacova 5 min, 15 min, 2 h, 48 h i 7 dana nakon aplikacije. U svim praćenim moždanim strukturama neuroprotektivno je delovala primena CNQX i APV u smislu smanjenja produkcije NO, ali sa očiglednom razlikom u mehanizmu dejstva i vremenskoj dinamici. Rezultati našeg istraživanja dokazuju da su glutamatski NMDA i AMPA/kainatni receptori različito uključeni u proces produkcije NO.",
journal = "Archives of Biological Sciences",
title = "Efekat antagonista glutamata na stvaranje azot oksida u mozgu pacova, Effect of glutamate antagonists on nitric oxide production in rat brain following intrahippocampal injection",
number = "1",
volume = "59",
pages = "29-36",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_146"
}

Radenović, L., Todorović, D., Selaković, V.,& Petković, B.. (2007). Efekat antagonista glutamata na stvaranje azot oksida u mozgu pacova. in Archives of Biological Sciences, 59(1), 29-36.
https://hdl.handle.net/21.15107/rcub_ibiss_146

Radenović L, Todorović D, Selaković V, Petković B. Efekat antagonista glutamata na stvaranje azot oksida u mozgu pacova. in Archives of Biological Sciences. 2007;59(1):29-36.
https://hdl.handle.net/21.15107/rcub_ibiss_146 .

Radenović, Lidija, Todorović, Dajana, Selaković, Vesna, Petković, Branka, "Efekat antagonista glutamata na stvaranje azot oksida u mozgu pacova" in Archives of Biological Sciences, 59, no. 1 (2007):29-36,
https://hdl.handle.net/21.15107/rcub_ibiss_146 .

TY  - JOUR
AU  - Radenović, Lidija
AU  - Nedeljković, M.
AU  - Kartelija, Gordana
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/115
AB  - Using the single electrode voltage clamp method, we found that acetylcholine (aCh) induces transient inward dose-dependent current on the membrane of the identified Helix pomatia Br neuron. We analyzed the effects of fast cooling and heating as well as thermal acclimation on the aCh inward current. the experiments were conducted on active and dormant snails acclimated to either 20 or 7°C for at least four weeks. the Hill coefficient remained approximately 1 in all cases, which means that there is a single aCh binding site on the membrane. Fast temperature alternations induce binding affinity changes. in the work presented, we analyzed the effects of cooling on the aCh-induced inward current. the amplitude of aCh-induced inward current was markedly reduced after cooling, and the speed of decay of the aCh response was lower.
AB  - Upotrebom metode nametnute voltaže ustanovili smo da acetilholin (aCh) izaziva dozno-zavisnu ulaznu struju na membrani identifikovanog Br neurona puža Helix pomatia. Analizirali smo efekte brzog hlađenja i zagrevanja kao i termalne aklimacije na aCh ulaznu struju. eksperimenti su rađeni na puževima aklimiranim na 20 i 7°C u toku četiri nedelje. Hill-ov koeficijent je uvek ostajao oko 1, što znači da postoji samo jedno mesto za vezivanje aCh na membrani neurona. Aklimacija na 7°C nije menjala afinitet za vezivanje aCh za razliku od aklimacije na 20°C gde je ustanovljeno povećanje afiniteta za vezivanje aCh na membrani identifikovanog Br neurona puža Helix pomatia.
T2  - Archives of Biological Sciences
T1  - Modifikacija aCh-indukovane struje brzim promenama temperature kod puža Helix pomatia L.
T1  - Modification of the acetylcholine-induced current of the snail Helix pomatia L. by fast temperature changes
IS  - 3
VL  - 57
SP  - 181
EP  - 187
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_115
ER  - 

@article{
author = "Radenović, Lidija and Nedeljković, M. and Kartelija, Gordana",
year = "2005, 2005",
abstract = "Using the single electrode voltage clamp method, we found that acetylcholine (aCh) induces transient inward dose-dependent current on the membrane of the identified Helix pomatia Br neuron. We analyzed the effects of fast cooling and heating as well as thermal acclimation on the aCh inward current. the experiments were conducted on active and dormant snails acclimated to either 20 or 7°C for at least four weeks. the Hill coefficient remained approximately 1 in all cases, which means that there is a single aCh binding site on the membrane. Fast temperature alternations induce binding affinity changes. in the work presented, we analyzed the effects of cooling on the aCh-induced inward current. the amplitude of aCh-induced inward current was markedly reduced after cooling, and the speed of decay of the aCh response was lower., Upotrebom metode nametnute voltaže ustanovili smo da acetilholin (aCh) izaziva dozno-zavisnu ulaznu struju na membrani identifikovanog Br neurona puža Helix pomatia. Analizirali smo efekte brzog hlađenja i zagrevanja kao i termalne aklimacije na aCh ulaznu struju. eksperimenti su rađeni na puževima aklimiranim na 20 i 7°C u toku četiri nedelje. Hill-ov koeficijent je uvek ostajao oko 1, što znači da postoji samo jedno mesto za vezivanje aCh na membrani neurona. Aklimacija na 7°C nije menjala afinitet za vezivanje aCh za razliku od aklimacije na 20°C gde je ustanovljeno povećanje afiniteta za vezivanje aCh na membrani identifikovanog Br neurona puža Helix pomatia.",
journal = "Archives of Biological Sciences",
title = "Modifikacija aCh-indukovane struje brzim promenama temperature kod puža Helix pomatia L., Modification of the acetylcholine-induced current of the snail Helix pomatia L. by fast temperature changes",
number = "3",
volume = "57",
pages = "181-187",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_115"
}

Radenović, L., Nedeljković, M.,& Kartelija, G.. (2005). Modifikacija aCh-indukovane struje brzim promenama temperature kod puža Helix pomatia L.. in Archives of Biological Sciences, 57(3), 181-187.
https://hdl.handle.net/21.15107/rcub_ibiss_115

Radenović L, Nedeljković M, Kartelija G. Modifikacija aCh-indukovane struje brzim promenama temperature kod puža Helix pomatia L.. in Archives of Biological Sciences. 2005;57(3):181-187.
https://hdl.handle.net/21.15107/rcub_ibiss_115 .

Radenović, Lidija, Nedeljković, M., Kartelija, Gordana, "Modifikacija aCh-indukovane struje brzim promenama temperature kod puža Helix pomatia L." in Archives of Biological Sciences, 57, no. 3 (2005):181-187,
https://hdl.handle.net/21.15107/rcub_ibiss_115 .

TY  - JOUR
AU  - Radenović, Lidija
AU  - Kartelija, Gordana
PY  - 2000
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/457
AB  - Chlorpromazine (CPZ), an antipsychotic drug, was found to inhibit intracellular carboxylesterases (CarbEs). As intracellular target carboxylesterases we used alpha-naphthyl acetate esterase (alpha-NA), naphthol AS-D chloroacetate esterase (AS-D) and alpha-naphthyl butyrate esterase (alpha-NB) in mouse polymorphonuclear neutrophils (PMN), hepatocytes (HC) and neuronal brain cells (NC). The impact of CPZ on the cells ranged from no effect to death, with intermediary effects of decreased CarbEs activities without either morphological changes or structural changes. The results of our study indicate that intracellular CarbEs activity inhibition by CPZ was dose-dependent, though the drug concentration required to bring about 50% inhibition of the initial activity (ID-50) varied between the mouse cell types, under the same experimental conditions. CarbEs activity was decreased or completely inhibited at CPZ concentrations ranging from 0.5 to 5.0 mg/ml (1.4 to 14.08 mmol/l). The impact maximum concentration of CPZ 5 mg/ml (14.08 mmol/l) on mouse brain cells resulted in 46.58% inhibition for AS-D, 54.26% for α-NA and 99.52% for α-NB. Our studies established a clear relationship between the increasing concentrations of CPZ and the extent of inhibition of the intracellular esterases of mice. Correlation of the inhibitory effects in all the cell types was demonstrated. The polymorphonuclear neutrophils - leukocytes were the most sensitive (ID-50 = 0.42 mg CPZ/ml) and the hepatocytes most resistant to the CPZ effect (ID-50 = 2.45 mg CPZ/ml). Since leukocytes are human cells much more readily available than hepatocytes or neuronal cells, we presume that CarbEs in peripheral blood leukocytes could be used as markers for the indication of intracellular biochemical damage of hepatocytes and neuronal brain cells by CPZ.
AB  - Hlorpromazin (CPZ) je neuroleptik koji se koristi za dugotrajni tretman najtežih psihijatrijskih bolesti. Osnovni mehanizam dejstva CPZ je blokada D2 receptora ali se malo zna o efektima CPZ na intracelularnu biohemijsku aktivnost moždanog tkiva. U ovom radu ispitivan je uticaj CPZ-a na intracelularne karboksilesteraze (EC 3.1.1.1.), kao marker biohemijske aktivnosti. Primenom osetljive in vitro metode - MIMDECI detektovane su rane intracelularne biohemijske promene u morfološki očuvanim ćelijama, indukovane primenom CPZ-a. MIMDECI je tehnologija koja koristi dva markera (za enzimsku aktivnost i morfologiju ćelija) i meri delovanje leka na enzim pre pojave nespecifičnog oštećenja ćelija. Intracelularna biohemijska aktivnost praćena je pomoću alfa-naftil acetat karboksilesteraze, naftol AS-D hloroacetat karboksilesteraze i alfa-naftil butarat karboksilesteraze. Karboksilesteraze su citoplazmatski konstituenti mnogih tipova ćelija, tako da su paralelno praćeni efekti CPZ-a u polimorfonuklearima periferne krvi, hepatocitima i nervnim ćelijama mozga sivog miša, u in vitro uslovima. Naši rezultati ukazuju da CPZ inhibira karboksilesteraze u sva tri tipa ćelija i da stepen inhibicije zavisi od vremena aplikacije i primenjene koncentracije. Intraceiularna kar boksiiesterazna aktivnost je smanjena ili potpuno inhibirana primenom CPZ-a u rastućim koncentracijama od 0.5 - 5.0 mg/ml. Korelacijom inhibitornog efekta u sva tri tipa ćelija ustanovili smo da su polimorfonuklearni leukociti periferne krvi najosetljiviji na dejstvo CPZ-a (ID50 = 0.42 mg/ml) dok su hepatociti najrezistentniji (ID50 = 2.45 mg/ml).
T2  - Acta veterinaria
T1  - Hronično unošenje hlorpromazina izaziva inhibiciju intracelularne biohemijske aktivnosti moždanog tkiva miša
T1  - Chlorpromazine treatment induced inhibition of intracellular biochemical activity of mouse brain tissue
IS  - 5-6
VL  - 50
SP  - 361
EP  - 373
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_457
ER  - 

@article{
author = "Radenović, Lidija and Kartelija, Gordana",
year = "2000, 2000",
abstract = "Chlorpromazine (CPZ), an antipsychotic drug, was found to inhibit intracellular carboxylesterases (CarbEs). As intracellular target carboxylesterases we used alpha-naphthyl acetate esterase (alpha-NA), naphthol AS-D chloroacetate esterase (AS-D) and alpha-naphthyl butyrate esterase (alpha-NB) in mouse polymorphonuclear neutrophils (PMN), hepatocytes (HC) and neuronal brain cells (NC). The impact of CPZ on the cells ranged from no effect to death, with intermediary effects of decreased CarbEs activities without either morphological changes or structural changes. The results of our study indicate that intracellular CarbEs activity inhibition by CPZ was dose-dependent, though the drug concentration required to bring about 50% inhibition of the initial activity (ID-50) varied between the mouse cell types, under the same experimental conditions. CarbEs activity was decreased or completely inhibited at CPZ concentrations ranging from 0.5 to 5.0 mg/ml (1.4 to 14.08 mmol/l). The impact maximum concentration of CPZ 5 mg/ml (14.08 mmol/l) on mouse brain cells resulted in 46.58% inhibition for AS-D, 54.26% for α-NA and 99.52% for α-NB. Our studies established a clear relationship between the increasing concentrations of CPZ and the extent of inhibition of the intracellular esterases of mice. Correlation of the inhibitory effects in all the cell types was demonstrated. The polymorphonuclear neutrophils - leukocytes were the most sensitive (ID-50 = 0.42 mg CPZ/ml) and the hepatocytes most resistant to the CPZ effect (ID-50 = 2.45 mg CPZ/ml). Since leukocytes are human cells much more readily available than hepatocytes or neuronal cells, we presume that CarbEs in peripheral blood leukocytes could be used as markers for the indication of intracellular biochemical damage of hepatocytes and neuronal brain cells by CPZ., Hlorpromazin (CPZ) je neuroleptik koji se koristi za dugotrajni tretman najtežih psihijatrijskih bolesti. Osnovni mehanizam dejstva CPZ je blokada D2 receptora ali se malo zna o efektima CPZ na intracelularnu biohemijsku aktivnost moždanog tkiva. U ovom radu ispitivan je uticaj CPZ-a na intracelularne karboksilesteraze (EC 3.1.1.1.), kao marker biohemijske aktivnosti. Primenom osetljive in vitro metode - MIMDECI detektovane su rane intracelularne biohemijske promene u morfološki očuvanim ćelijama, indukovane primenom CPZ-a. MIMDECI je tehnologija koja koristi dva markera (za enzimsku aktivnost i morfologiju ćelija) i meri delovanje leka na enzim pre pojave nespecifičnog oštećenja ćelija. Intracelularna biohemijska aktivnost praćena je pomoću alfa-naftil acetat karboksilesteraze, naftol AS-D hloroacetat karboksilesteraze i alfa-naftil butarat karboksilesteraze. Karboksilesteraze su citoplazmatski konstituenti mnogih tipova ćelija, tako da su paralelno praćeni efekti CPZ-a u polimorfonuklearima periferne krvi, hepatocitima i nervnim ćelijama mozga sivog miša, u in vitro uslovima. Naši rezultati ukazuju da CPZ inhibira karboksilesteraze u sva tri tipa ćelija i da stepen inhibicije zavisi od vremena aplikacije i primenjene koncentracije. Intraceiularna kar boksiiesterazna aktivnost je smanjena ili potpuno inhibirana primenom CPZ-a u rastućim koncentracijama od 0.5 - 5.0 mg/ml. Korelacijom inhibitornog efekta u sva tri tipa ćelija ustanovili smo da su polimorfonuklearni leukociti periferne krvi najosetljiviji na dejstvo CPZ-a (ID50 = 0.42 mg/ml) dok su hepatociti najrezistentniji (ID50 = 2.45 mg/ml).",
journal = "Acta veterinaria",
title = "Hronično unošenje hlorpromazina izaziva inhibiciju intracelularne biohemijske aktivnosti moždanog tkiva miša, Chlorpromazine treatment induced inhibition of intracellular biochemical activity of mouse brain tissue",
number = "5-6",
volume = "50",
pages = "361-373",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_457"
}

Radenović, L.,& Kartelija, G.. (2000). Hronično unošenje hlorpromazina izaziva inhibiciju intracelularne biohemijske aktivnosti moždanog tkiva miša. in Acta veterinaria, 50(5-6), 361-373.
https://hdl.handle.net/21.15107/rcub_ibiss_457

Radenović L, Kartelija G. Hronično unošenje hlorpromazina izaziva inhibiciju intracelularne biohemijske aktivnosti moždanog tkiva miša. in Acta veterinaria. 2000;50(5-6):361-373.
https://hdl.handle.net/21.15107/rcub_ibiss_457 .

Radenović, Lidija, Kartelija, Gordana, "Hronično unošenje hlorpromazina izaziva inhibiciju intracelularne biohemijske aktivnosti moždanog tkiva miša" in Acta veterinaria, 50, no. 5-6 (2000):361-373,
https://hdl.handle.net/21.15107/rcub_ibiss_457 .