TY  - CONF
AU  - Janković-Tomanić, Milena
AU  - Vranković, Jelena
AU  - Petković, Branka
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6525
AB  - Tenebrio molitor is considered a pest of stored grains and grain-based products, but also as an important
supplement in animal and human nutrition. It is important to point out that T. molitor larvae are sensitive
to the presence of mycotoxins in the environment, changing the behavior and some biochemical
parameters. Mycotoxin zearalenone (ZEA) was used to assess the effect on the induction of oxidative
stress and behavior in T. molitor larvae. The aim of this research was to examine the effect of high doses
of mycotoxin ZEA (10 and 20 mg/kg) in feed on the activity of the enzymes superoxide dismutase (SOD),
catalase (CAT), and glutathione S-transferase (GST), as well as on the parameters of locomotor activity:
travel distance, time in movement, and average speed while in motion in T. molitor larvae.
The results of this study showed that the presence of ZEA in feed at concentrations of 10 and 20 mg/kg
significantly increased the specific activity of SOD, CAT, and GST. It also affected the locomotor activity
of the larvae, i.e. travel distance and time in movement were significantly lower in larvae exposed to
10 and 20 mg/kg ZEA, while average speed did not change significantly. The presence of ZEA leads to
significant changes in the physiology and behavior of T. molitor larvae and can be useful in the early
detection of food contamination with this dangerous mycotoxin.
AB  - Tenebrio molitor se smatra štetočinom uskladištenih žitarica i proizvoda od žitarica, ali i kao važan
dodatak u ishrani životinja i ljudi. Važno je istaći da su T. molitor larve osetljive na prisustvo mikotoksina
u okruženju, menjajući ponašanje i vrednosti biohemijskih parametara. Fungalni toksin zearalenon
(ZEA) je korišćen za procenu uticaja na oksidativni stres i lokomotornu aktivnost larvi brašnenog crva, T.
molitor. Cilj ovog istraživanja je bio da se ispita uticaj visokih doza mikotoksina ZEA (10 i 20 mg/kg) u hrani
na aktivnost enzima superoksid dismutaze (SOD), katalaze (CAT) i glutation S-transferaze (GST), kao i na
parametre lokomotorne aktivnosti: pređeni put, vreme kretanja i prosečnu brzinu kretanja larvi T. molitor.
Rezultati ovog istraživanja su pokazali da prisustvo ZEA u hrani u koncentracijama od 10 i 20 mg/kg
značajno povećava specifičnu aktivnost SOD, CAT i GST. Takođe, uticalo je i na lokomotornu aktivnost
larvi tj. pređeni put i vreme kretanja su bili značajno niži kod larvi izloženih 10 i 20 mg/kg ZEA, dok se
prosečna brzina kretanja nije značajno menjala.
Prisustvo ZEA dovodi do značajnih promena u fiziologiji i ponašanju larvi T. molitor i može biti korisno
u ranom otkrivanju kontaminacije hrane ovim opasnim mikotoksinom.
PB  - Belgrade: Serbian Society of Toxicology
C3  - Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
T1  - The effects of high doses of the mycotoxin zearalenone on Tenebrio molitor L. larvae
T1  - Efekat visokih doza mikotoksina zearalenona na larve brašnenog crva Tenebrio molitor L.
SP  - 87
EP  - 88
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6525
ER  - 

@conference{
author = "Janković-Tomanić, Milena and Vranković, Jelena and Petković, Branka and Perić Mataruga, Vesna",
year = "2023",
abstract = "Tenebrio molitor is considered a pest of stored grains and grain-based products, but also as an important
supplement in animal and human nutrition. It is important to point out that T. molitor larvae are sensitive
to the presence of mycotoxins in the environment, changing the behavior and some biochemical
parameters. Mycotoxin zearalenone (ZEA) was used to assess the effect on the induction of oxidative
stress and behavior in T. molitor larvae. The aim of this research was to examine the effect of high doses
of mycotoxin ZEA (10 and 20 mg/kg) in feed on the activity of the enzymes superoxide dismutase (SOD),
catalase (CAT), and glutathione S-transferase (GST), as well as on the parameters of locomotor activity:
travel distance, time in movement, and average speed while in motion in T. molitor larvae.
The results of this study showed that the presence of ZEA in feed at concentrations of 10 and 20 mg/kg
significantly increased the specific activity of SOD, CAT, and GST. It also affected the locomotor activity
of the larvae, i.e. travel distance and time in movement were significantly lower in larvae exposed to
10 and 20 mg/kg ZEA, while average speed did not change significantly. The presence of ZEA leads to
significant changes in the physiology and behavior of T. molitor larvae and can be useful in the early
detection of food contamination with this dangerous mycotoxin., Tenebrio molitor se smatra štetočinom uskladištenih žitarica i proizvoda od žitarica, ali i kao važan
dodatak u ishrani životinja i ljudi. Važno je istaći da su T. molitor larve osetljive na prisustvo mikotoksina
u okruženju, menjajući ponašanje i vrednosti biohemijskih parametara. Fungalni toksin zearalenon
(ZEA) je korišćen za procenu uticaja na oksidativni stres i lokomotornu aktivnost larvi brašnenog crva, T.
molitor. Cilj ovog istraživanja je bio da se ispita uticaj visokih doza mikotoksina ZEA (10 i 20 mg/kg) u hrani
na aktivnost enzima superoksid dismutaze (SOD), katalaze (CAT) i glutation S-transferaze (GST), kao i na
parametre lokomotorne aktivnosti: pređeni put, vreme kretanja i prosečnu brzinu kretanja larvi T. molitor.
Rezultati ovog istraživanja su pokazali da prisustvo ZEA u hrani u koncentracijama od 10 i 20 mg/kg
značajno povećava specifičnu aktivnost SOD, CAT i GST. Takođe, uticalo je i na lokomotornu aktivnost
larvi tj. pređeni put i vreme kretanja su bili značajno niži kod larvi izloženih 10 i 20 mg/kg ZEA, dok se
prosečna brzina kretanja nije značajno menjala.
Prisustvo ZEA dovodi do značajnih promena u fiziologiji i ponašanju larvi T. molitor i može biti korisno
u ranom otkrivanju kontaminacije hrane ovim opasnim mikotoksinom.",
publisher = "Belgrade: Serbian Society of Toxicology",
journal = "Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia",
title = "The effects of high doses of the mycotoxin zearalenone on Tenebrio molitor L. larvae, Efekat visokih doza mikotoksina zearalenona na larve brašnenog crva Tenebrio molitor L.",
pages = "87-88",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6525"
}

Janković-Tomanić, M., Vranković, J., Petković, B.,& Perić Mataruga, V.. (2023). The effects of high doses of the mycotoxin zearalenone on Tenebrio molitor L. larvae. in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia
Belgrade: Serbian Society of Toxicology., 87-88.
https://hdl.handle.net/21.15107/rcub_ibiss_6525

Janković-Tomanić M, Vranković J, Petković B, Perić Mataruga V. The effects of high doses of the mycotoxin zearalenone on Tenebrio molitor L. larvae. in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia. 2023;:87-88.
https://hdl.handle.net/21.15107/rcub_ibiss_6525 .

Janković-Tomanić, Milena, Vranković, Jelena, Petković, Branka, Perić Mataruga, Vesna, "The effects of high doses of the mycotoxin zearalenone on Tenebrio molitor L. larvae" in Abstract Book: 13th International Congress of the Serbian Society of Toxicology and 1st toxSEE Regional Conference: Present and Future of toxicology: Challenges and opportunities; 2023 May 10-12; Belgrade, Serbia (2023):87-88,
https://hdl.handle.net/21.15107/rcub_ibiss_6525 .

TY  - JOUR
AU  - Janković Tomanić, Milena
AU  - Petković, Branka
AU  - Vranković, Jelena
AU  - Perić Mataruga, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5730
AB  - The larvae of the yellow mealworm Tenebrio molitor represent a new sustainable source of protein and nutrients for animal and human nutrition. To ensure safe insect-based foods, the risks of mycotoxins and other chemicals must be elucidated. Since oxidative stress is known to underlie many mycotoxin-induced disorders, the present work investigates the effects of the mycotoxin zearalenone (ZEA) on the responses of some oxidative stress biomarkers, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) in the whole body of mealworm, as well as the effects on larval survival, body weight, and locomotor activity, and some aspects of mycotoxin accumulation. Larvae were fed artificially contaminated wheat bran containing approximately 0.1 mg/kg, 0.5 mg/kg, 2 mg/kg, and 5 mg/kg ZEA for two weeks. The results of the current study showed that ZEA-contaminated wheat bran at a concentration of 0.5 mg/kg was correlated with increased SOD, CAT, and GST enzyme activities and decreased locomotor activity. Higher concentrations of ZEA, i.e., 2 and 5 mg/kg caused enzymes and locomotor activity to return to control levels. The presence of ZEA in the diet for two weeks did not affect survival but resulted in lower weight gain in T. molitor larvae. The concentration of ZEA in the residual material was 21.1–37.6%, while in the larvae it accounted for 0.38–0.51% of the mycotoxin in the feed. The selected concentrations were in accordance with the EU limits for the presence of ZEA in food and with the levels found in naturally contaminated cereals. Our results suggest that ZEA at the tested concentration induces physiological, biochemical, and behavioral changes in larvae that could potentially serve as early markers of mycotoxin contamination.
PB  - Oxford: Pergamon-Elsevier Science Ltd.
T2  - Journal of Stored Products Research
T1  - Changes in antioxidant enzymes and locomotor activity of yellow mealworm larvae fed the mycotoxin zearalenone supplemented diet
VL  - 102
DO  - 10.1016/j.jspr.2023.102113
SP  - 102113
ER  - 

@article{
author = "Janković Tomanić, Milena and Petković, Branka and Vranković, Jelena and Perić Mataruga, Vesna",
year = "2023",
abstract = "The larvae of the yellow mealworm Tenebrio molitor represent a new sustainable source of protein and nutrients for animal and human nutrition. To ensure safe insect-based foods, the risks of mycotoxins and other chemicals must be elucidated. Since oxidative stress is known to underlie many mycotoxin-induced disorders, the present work investigates the effects of the mycotoxin zearalenone (ZEA) on the responses of some oxidative stress biomarkers, superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) in the whole body of mealworm, as well as the effects on larval survival, body weight, and locomotor activity, and some aspects of mycotoxin accumulation. Larvae were fed artificially contaminated wheat bran containing approximately 0.1 mg/kg, 0.5 mg/kg, 2 mg/kg, and 5 mg/kg ZEA for two weeks. The results of the current study showed that ZEA-contaminated wheat bran at a concentration of 0.5 mg/kg was correlated with increased SOD, CAT, and GST enzyme activities and decreased locomotor activity. Higher concentrations of ZEA, i.e., 2 and 5 mg/kg caused enzymes and locomotor activity to return to control levels. The presence of ZEA in the diet for two weeks did not affect survival but resulted in lower weight gain in T. molitor larvae. The concentration of ZEA in the residual material was 21.1–37.6%, while in the larvae it accounted for 0.38–0.51% of the mycotoxin in the feed. The selected concentrations were in accordance with the EU limits for the presence of ZEA in food and with the levels found in naturally contaminated cereals. Our results suggest that ZEA at the tested concentration induces physiological, biochemical, and behavioral changes in larvae that could potentially serve as early markers of mycotoxin contamination.",
publisher = "Oxford: Pergamon-Elsevier Science Ltd.",
journal = "Journal of Stored Products Research",
title = "Changes in antioxidant enzymes and locomotor activity of yellow mealworm larvae fed the mycotoxin zearalenone supplemented diet",
volume = "102",
doi = "10.1016/j.jspr.2023.102113",
pages = "102113"
}

Janković Tomanić, M., Petković, B., Vranković, J.,& Perić Mataruga, V.. (2023). Changes in antioxidant enzymes and locomotor activity of yellow mealworm larvae fed the mycotoxin zearalenone supplemented diet. in Journal of Stored Products Research
Oxford: Pergamon-Elsevier Science Ltd.., 102, 102113.
https://doi.org/10.1016/j.jspr.2023.102113

Janković Tomanić M, Petković B, Vranković J, Perić Mataruga V. Changes in antioxidant enzymes and locomotor activity of yellow mealworm larvae fed the mycotoxin zearalenone supplemented diet. in Journal of Stored Products Research. 2023;102:102113.
doi:10.1016/j.jspr.2023.102113 .

Janković Tomanić, Milena, Petković, Branka, Vranković, Jelena, Perić Mataruga, Vesna, "Changes in antioxidant enzymes and locomotor activity of yellow mealworm larvae fed the mycotoxin zearalenone supplemented diet" in Journal of Stored Products Research, 102 (2023):102113,
https://doi.org/10.1016/j.jspr.2023.102113 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Milanović, Slobodan
AU  - Šešlija Jovanović, Darka
AU  - Janković Tomanić, Milena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5721
AB  - Temperature and food quality are the most important environmental factors determining
the performance of herbivorous insects. The objective of our study was to evaluate the responses of
the spongy moth (formerly known as the gypsy moth) [Lymantria dispar L. (Lepidoptera: Erebidae)] to
simultaneous variation in these two factors. From hatching to the fourth instar, larvae were exposed
to three temperatures (19  C, 23  C, and 28  C) and fed four artificial diets that differed in protein (P)
and carbohydrate (C) content. Within each temperature regime, the effects of the nutrient content
(P+C) and ratio (P:C) on development duration, larval mass, growth rate, and activities of digestive
proteases, carbohydrases, and lipase were examined. It was found that temperature and food quality
had a significant effect on the fitness-related traits and digestive physiology of the larvae. The greatest
mass and highest growth rate were obtained at 28  C on a high-protein low-carbohydrate diet. A
homeostatic increase in activity was observed for total protease, trypsin, and amylase in response to
low substrate levels in the diet. A significant modulation of overall enzyme activities in response
to 28  C was detected only with a low diet quality. A decrease in the nutrient content and P:C ratio
only affected the coordination of enzyme activities at 28  C, as indicated by the significantly altered
correlation matrices. Multiple linear regression analysis showed that variation in fitness traits in
response to different rearing conditions could be explained by variation in digestion. Our results
contribute to the understanding of the role of digestive enzymes in post-ingestive nutrient balancing
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae
IS  - 5
VL  - 13
DO  - 10.3390/biom13050821
SP  - 821
ER  - 

@article{
author = "Lazarević, Jelica and Milanović, Slobodan and Šešlija Jovanović, Darka and Janković Tomanić, Milena",
year = "2023",
abstract = "Temperature and food quality are the most important environmental factors determining
the performance of herbivorous insects. The objective of our study was to evaluate the responses of
the spongy moth (formerly known as the gypsy moth) [Lymantria dispar L. (Lepidoptera: Erebidae)] to
simultaneous variation in these two factors. From hatching to the fourth instar, larvae were exposed
to three temperatures (19  C, 23  C, and 28  C) and fed four artificial diets that differed in protein (P)
and carbohydrate (C) content. Within each temperature regime, the effects of the nutrient content
(P+C) and ratio (P:C) on development duration, larval mass, growth rate, and activities of digestive
proteases, carbohydrases, and lipase were examined. It was found that temperature and food quality
had a significant effect on the fitness-related traits and digestive physiology of the larvae. The greatest
mass and highest growth rate were obtained at 28  C on a high-protein low-carbohydrate diet. A
homeostatic increase in activity was observed for total protease, trypsin, and amylase in response to
low substrate levels in the diet. A significant modulation of overall enzyme activities in response
to 28  C was detected only with a low diet quality. A decrease in the nutrient content and P:C ratio
only affected the coordination of enzyme activities at 28  C, as indicated by the significantly altered
correlation matrices. Multiple linear regression analysis showed that variation in fitness traits in
response to different rearing conditions could be explained by variation in digestion. Our results
contribute to the understanding of the role of digestive enzymes in post-ingestive nutrient balancing",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae",
number = "5",
volume = "13",
doi = "10.3390/biom13050821",
pages = "821"
}

Lazarević, J., Milanović, S., Šešlija Jovanović, D.,& Janković Tomanić, M.. (2023). Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae. in Biomolecules
Basel: MDPI., 13(5), 821.
https://doi.org/10.3390/biom13050821

Lazarević J, Milanović S, Šešlija Jovanović D, Janković Tomanić M. Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae. in Biomolecules. 2023;13(5):821.
doi:10.3390/biom13050821 .

Lazarević, Jelica, Milanović, Slobodan, Šešlija Jovanović, Darka, Janković Tomanić, Milena, "Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae" in Biomolecules, 13, no. 5 (2023):821,
https://doi.org/10.3390/biom13050821 . .

TY  - CONF
AU  - Janković Tomanić, Milena
AU  - Vranković, Jelena
AU  - Petković, Branka
AU  - Perić Mataruga, Vesna
PY  - 2022
UR  - http://www.eds.org.rs/SES/2022/Zbornik%20rezimea%20XIII%20SES%202022.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5740
AB  - Tenebrio molitor L. (Coleoptera, Tenebrionidae) (brašneni crv) je holometabolni insekt, koji se smatra jednim od glavnih štetočina silosa i žitarica. Sa druge strane, on se može smatrati i korisnom vrstom, obzirom da je izvor visoko kvalitetnih nutrienata u ishrani životinja, sa perspektivom korišćenja i u ljudskoj ishrani. Takođe je veoma važno istaći da su larve T. molitor osetljive na prisustvo mikotoksina u okruženju. Larve menjaju ponašanje u njihovom prisustvu, događaju se brze biohemijske i fiziološke promene koje ga prate, tako da vrsta T. molitor može imati potencijal za detektovanje mikotoksina u hrani. Za ovu studiju, izabran je mikotoksin zearalenon (ZEA), koji predstavlja sekundarni metabolit gljiva iz roda Fusarium, najčešće F. culmorum i F. graminearum. ZEA je pronañen u uzorcima pšenice i kukuruza širom Evrope u koncentracijama u rasponu od 15 µg/kg do 5700 µg/kg, pa čak i do 14,58 mg/kg (Fruhauf i sar., 2019). Cilj ovog istraživanja je bio da se ispita uticaj mikotoksina dodatog u pšenične mekinje, koje predstavljaju optimalan supstrat za gajenje larvi T. molitor, na aktivnost enzima antioksidativne zaštite, superoksid dismutaze (SOD) i katalaze (CAT), detoksifikacionog enzima glutation-S-transferaze (GST) kao i motornog ponašanje larvi (pređeni put, vreme provedeno u kretanju i prosečna brzina tokom kretanja). Primenjene doze mikotoksina su bile sledeće: 100 µg/kg (što odgovara maksimalno dozvoljenoj vrednosti u kukuruzu namenjenom za neposrednu ishranu ljudi), 2 mg/kg (žitarice i proizvodi od žitarica osim kukuruza namenjeni za ishranu životinja; Preporuka Evropske komisije 2006/576/EC) i 10 mg/kg hrane (što odgovara maksimalnim vrednostima koje se mogu naći u uzorcima žitarica). Koristili smo larve iz populacije gajene u Institutu za biološka istraživanja „Siniša Stanković“, koje su na početku eksperimenta bile prosečne starosti 2 meseca i prosečne težine 106,2 ± 0,8 mg. Uočeno je da prisustvo mikotoksina u hrani značajno utiče na aktivnost enzima SOD (F(3, 50)=11,738, P<0,001) i GST (F(3,50)=7,889, P<0,001), dok ne dovodi do promene u aktivnosti enzima CAT (F(3, 50)=0,775, P=0,513). Aktivnosti enzima SOD i GST su bile značajno više u grupama larvi gajenih na supstratu sa najnižom (100 µg/kg) i najvišom (10 mg/kg) dozom mikotoksina u odnosu na kontrolne larve gajene na supstratu bez mikotoksina. U grupi larvi gajenih na supstratu sa 2 mg/kg mikotoksina, aktivnost ovih enzima je bila na kontrolnom nivou. Mikotoksin prisutan u hrani utiče i na motorno ponašanje jedinki značajno menjajući pređeni put (H(3,33)=8,739, P<0,05) i vreme provedeno u kretanju (H(3,33)=7,866, P<0,05), dok nema uticaja na prosečnu brzinu tokom kretanja (H(3,33)=5,525, P=0,137). Pređeni put i vreme provedeno u kretanju su bili značajno niži u grupama larvi gajenih na supstratu sa najnižom (100 µg/kg) i najvišom (10 mg/kg) dozom mikotoksina, i nepromenjeni u grupi larvi gajenih na supstratu sa 2 mg/kg mikotoksina, u odnosu na kontrolne larve. Naši rezultati ukazuju da prisustvo mikotoksina ZEA u hrani u dozama od 100 µg/kg i 10 mg/kg dovodi do promena u fiziologiji i ponašanju larvi T. molitor, i to povećanja aktivnosti antioksidativnog i detoksifikacionog enzima praćenog smanjenom motornom aktivnošću. Stoga, analiza ovih lako merljivih i osetljivih parametara može biti važna u ispitivanju i ranom otkrivanju kontaminacije hrane ovim veoma opasnim mikotoksinom.
PB  - Belgrade: Entomological Society of Serbia
C3  - Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia
T1  - Uticaj mikotoksina zearalenona na aktivnost enzima antioksidativne zaštite i ponašanje larvi Tenebrio molitor (L.)
SP  - 38
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5740
ER  - 

@conference{
author = "Janković Tomanić, Milena and Vranković, Jelena and Petković, Branka and Perić Mataruga, Vesna",
year = "2022",
abstract = "Tenebrio molitor L. (Coleoptera, Tenebrionidae) (brašneni crv) je holometabolni insekt, koji se smatra jednim od glavnih štetočina silosa i žitarica. Sa druge strane, on se može smatrati i korisnom vrstom, obzirom da je izvor visoko kvalitetnih nutrienata u ishrani životinja, sa perspektivom korišćenja i u ljudskoj ishrani. Takođe je veoma važno istaći da su larve T. molitor osetljive na prisustvo mikotoksina u okruženju. Larve menjaju ponašanje u njihovom prisustvu, događaju se brze biohemijske i fiziološke promene koje ga prate, tako da vrsta T. molitor može imati potencijal za detektovanje mikotoksina u hrani. Za ovu studiju, izabran je mikotoksin zearalenon (ZEA), koji predstavlja sekundarni metabolit gljiva iz roda Fusarium, najčešće F. culmorum i F. graminearum. ZEA je pronañen u uzorcima pšenice i kukuruza širom Evrope u koncentracijama u rasponu od 15 µg/kg do 5700 µg/kg, pa čak i do 14,58 mg/kg (Fruhauf i sar., 2019). Cilj ovog istraživanja je bio da se ispita uticaj mikotoksina dodatog u pšenične mekinje, koje predstavljaju optimalan supstrat za gajenje larvi T. molitor, na aktivnost enzima antioksidativne zaštite, superoksid dismutaze (SOD) i katalaze (CAT), detoksifikacionog enzima glutation-S-transferaze (GST) kao i motornog ponašanje larvi (pređeni put, vreme provedeno u kretanju i prosečna brzina tokom kretanja). Primenjene doze mikotoksina su bile sledeće: 100 µg/kg (što odgovara maksimalno dozvoljenoj vrednosti u kukuruzu namenjenom za neposrednu ishranu ljudi), 2 mg/kg (žitarice i proizvodi od žitarica osim kukuruza namenjeni za ishranu životinja; Preporuka Evropske komisije 2006/576/EC) i 10 mg/kg hrane (što odgovara maksimalnim vrednostima koje se mogu naći u uzorcima žitarica). Koristili smo larve iz populacije gajene u Institutu za biološka istraživanja „Siniša Stanković“, koje su na početku eksperimenta bile prosečne starosti 2 meseca i prosečne težine 106,2 ± 0,8 mg. Uočeno je da prisustvo mikotoksina u hrani značajno utiče na aktivnost enzima SOD (F(3, 50)=11,738, P<0,001) i GST (F(3,50)=7,889, P<0,001), dok ne dovodi do promene u aktivnosti enzima CAT (F(3, 50)=0,775, P=0,513). Aktivnosti enzima SOD i GST su bile značajno više u grupama larvi gajenih na supstratu sa najnižom (100 µg/kg) i najvišom (10 mg/kg) dozom mikotoksina u odnosu na kontrolne larve gajene na supstratu bez mikotoksina. U grupi larvi gajenih na supstratu sa 2 mg/kg mikotoksina, aktivnost ovih enzima je bila na kontrolnom nivou. Mikotoksin prisutan u hrani utiče i na motorno ponašanje jedinki značajno menjajući pređeni put (H(3,33)=8,739, P<0,05) i vreme provedeno u kretanju (H(3,33)=7,866, P<0,05), dok nema uticaja na prosečnu brzinu tokom kretanja (H(3,33)=5,525, P=0,137). Pređeni put i vreme provedeno u kretanju su bili značajno niži u grupama larvi gajenih na supstratu sa najnižom (100 µg/kg) i najvišom (10 mg/kg) dozom mikotoksina, i nepromenjeni u grupi larvi gajenih na supstratu sa 2 mg/kg mikotoksina, u odnosu na kontrolne larve. Naši rezultati ukazuju da prisustvo mikotoksina ZEA u hrani u dozama od 100 µg/kg i 10 mg/kg dovodi do promena u fiziologiji i ponašanju larvi T. molitor, i to povećanja aktivnosti antioksidativnog i detoksifikacionog enzima praćenog smanjenom motornom aktivnošću. Stoga, analiza ovih lako merljivih i osetljivih parametara može biti važna u ispitivanju i ranom otkrivanju kontaminacije hrane ovim veoma opasnim mikotoksinom.",
publisher = "Belgrade: Entomological Society of Serbia",
journal = "Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia",
title = "Uticaj mikotoksina zearalenona na aktivnost enzima antioksidativne zaštite i ponašanje larvi Tenebrio molitor (L.)",
pages = "38",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5740"
}

Janković Tomanić, M., Vranković, J., Petković, B.,& Perić Mataruga, V.. (2022). Uticaj mikotoksina zearalenona na aktivnost enzima antioksidativne zaštite i ponašanje larvi Tenebrio molitor (L.). in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia
Belgrade: Entomological Society of Serbia., 38.
https://hdl.handle.net/21.15107/rcub_ibiss_5740

Janković Tomanić M, Vranković J, Petković B, Perić Mataruga V. Uticaj mikotoksina zearalenona na aktivnost enzima antioksidativne zaštite i ponašanje larvi Tenebrio molitor (L.). in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia. 2022;:38.
https://hdl.handle.net/21.15107/rcub_ibiss_5740 .

Janković Tomanić, Milena, Vranković, Jelena, Petković, Branka, Perić Mataruga, Vesna, "Uticaj mikotoksina zearalenona na aktivnost enzima antioksidativne zaštite i ponašanje larvi Tenebrio molitor (L.)" in Zbornik rezimea: 13. Simpozijum entomologa Srbije sa međunarodnim učešćem; 2022 Sep 14-16; Pirot, Serbia (2022):38,
https://hdl.handle.net/21.15107/rcub_ibiss_5740 .

TY  - JOUR
AU  - Vranković, Jelena
AU  - Janković Tomanić, Milena
AU  - Vukov, Tanja
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32376364
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3680
AB  - The traffic pressure is increasing, resulting in the emission of atmospheric pollution. Soil organisms will need to respond to pollution stressors. Among them, land snails are valuable indicators of ecosystem disturbance. In this study, land snails Helix pomatia were sampled from three city localities with different traffic intensity. Oxidative stress biomarkers catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and glutathione-S-transferase (GST) in the foot muscle (FM) and hepatopancreas (HP) tissue were determined. Also, five heavy metal (Cd, Cu, Ni, Pb, and Zn) concentrations were quantified in soil and tissue samples. According to the results, the highway induces the strongest contamination on the surrounding environment, with the highest metal concentrations measured in soil and snails. At the most polluted locality, only Cd exceeded some soil guidelines authorities that we referred to in this study. In addition, tissue Cd concentrations exceeded the United States Environmental Protection Agency (USEPA) value (1 mg kg-1) for soil invertebrate toxicity at all localities making it likely responsible for generating adverse effects in snails. Regarding HP, the CAT and GST are the most sensitive parameters that could be useful as oxidative stress biomarkers in snails exposed to the actual metals in the environment. On the other hand, in FM tissue, the most pronounced changes were recorded for GPX and GR. Based on tissue-specific enzyme responses, three urban populations were clearly separated. Therefore land snails are the promising candidates for quick field-based biomarker studies after showing a tissue-specific concentration-dependent induction of certain enzymes to heavy metals.
PB  - Elsevier BV
T2  - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
T1  - Comparative assessment of biomarker response to tissue metal concentrations in urban populations of the land snail Helix pomatia (Pulmonata: Helicidae).
VL  - 245
DO  - 10.1016/j.cbpb.2020.110448
SP  - 110448
ER  - 

@article{
author = "Vranković, Jelena and Janković Tomanić, Milena and Vukov, Tanja",
year = "2020",
abstract = "The traffic pressure is increasing, resulting in the emission of atmospheric pollution. Soil organisms will need to respond to pollution stressors. Among them, land snails are valuable indicators of ecosystem disturbance. In this study, land snails Helix pomatia were sampled from three city localities with different traffic intensity. Oxidative stress biomarkers catalase (CAT), glutathione peroxidase (GPX), glutathione reductase (GR) and glutathione-S-transferase (GST) in the foot muscle (FM) and hepatopancreas (HP) tissue were determined. Also, five heavy metal (Cd, Cu, Ni, Pb, and Zn) concentrations were quantified in soil and tissue samples. According to the results, the highway induces the strongest contamination on the surrounding environment, with the highest metal concentrations measured in soil and snails. At the most polluted locality, only Cd exceeded some soil guidelines authorities that we referred to in this study. In addition, tissue Cd concentrations exceeded the United States Environmental Protection Agency (USEPA) value (1 mg kg-1) for soil invertebrate toxicity at all localities making it likely responsible for generating adverse effects in snails. Regarding HP, the CAT and GST are the most sensitive parameters that could be useful as oxidative stress biomarkers in snails exposed to the actual metals in the environment. On the other hand, in FM tissue, the most pronounced changes were recorded for GPX and GR. Based on tissue-specific enzyme responses, three urban populations were clearly separated. Therefore land snails are the promising candidates for quick field-based biomarker studies after showing a tissue-specific concentration-dependent induction of certain enzymes to heavy metals.",
publisher = "Elsevier BV",
journal = "Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology",
title = "Comparative assessment of biomarker response to tissue metal concentrations in urban populations of the land snail Helix pomatia (Pulmonata: Helicidae).",
volume = "245",
doi = "10.1016/j.cbpb.2020.110448",
pages = "110448"
}

Vranković, J., Janković Tomanić, M.,& Vukov, T.. (2020). Comparative assessment of biomarker response to tissue metal concentrations in urban populations of the land snail Helix pomatia (Pulmonata: Helicidae).. in Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology
Elsevier BV., 245, 110448.
https://doi.org/10.1016/j.cbpb.2020.110448

Vranković J, Janković Tomanić M, Vukov T. Comparative assessment of biomarker response to tissue metal concentrations in urban populations of the land snail Helix pomatia (Pulmonata: Helicidae).. in Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology. 2020;245:110448.
doi:10.1016/j.cbpb.2020.110448 .

Vranković, Jelena, Janković Tomanić, Milena, Vukov, Tanja, "Comparative assessment of biomarker response to tissue metal concentrations in urban populations of the land snail Helix pomatia (Pulmonata: Helicidae)." in Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 245 (2020):110448,
https://doi.org/10.1016/j.cbpb.2020.110448 . .

TY  - JOUR
AU  - Janković Tomanić, Milena
AU  - Petković, Branka
AU  - Todorović, Dajana
AU  - Vranković, Jelena
AU  - Perić Mataruga, Vesna
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3447
AB  - Deoxynivalenol (DON) is one of the most prevalent type B trichothecene mycotoxins present in cereals such as: wheat, rye, barley, oats and corn. It initiates a wide range of toxic effects on human and animal health. As an edible insect species for the growing human population, Tenebrio molitor is typically fed on cereal bran or flour. In the present study T. molitor L. larvae were grown for two weeks on wheat bran artificially contaminated with DON at four concentrations: 4.9 μg/g, 8 μg/g, 16 μg/g and 25 μg/g. The effects of DON intake on survival, growth, activity of the antioxidant enzymes - superoxide dismutase (SOD) and catalase (CAT) and the phase II biotransformation enzyme - glutathione-S-transferase (GST), protein content and locomotor activity were monitored in two-month-old T. molitor larvae. The study revealed that DON at concentrations ranging from 4.9 to 25 μg/g wheat bran reduced larval body weight and protein content. Furthermore, it increased SOD and GST activity, had no effect on CAT activity. Also, this study showed that presence of DON in these concentrations has no direct detrimental effects on larval survival, but reduced locomotor activity. The observed effects were particularly pronounced in the larvae given the highest DON concentration 25 μg/g. These finding reveals that DON in artificially contaminated wheat bran at concentrations up to 25 μg/g is able to induce dose-dependent physiological and behavioral changes in T. molitor larvae.
PB  - Elsevier Ltd.
T2  - Journal of Stored Products Research
T1  - Physiological and behavioral effects of the mycotoxin deoxynivalenol in Tenebrio molitor larvae
VL  - 83
DO  - 10.1016/J.JSPR.2019.07.008
SP  - 236
EP  - 242
ER  - 

@article{
author = "Janković Tomanić, Milena and Petković, Branka and Todorović, Dajana and Vranković, Jelena and Perić Mataruga, Vesna",
year = "2019",
abstract = "Deoxynivalenol (DON) is one of the most prevalent type B trichothecene mycotoxins present in cereals such as: wheat, rye, barley, oats and corn. It initiates a wide range of toxic effects on human and animal health. As an edible insect species for the growing human population, Tenebrio molitor is typically fed on cereal bran or flour. In the present study T. molitor L. larvae were grown for two weeks on wheat bran artificially contaminated with DON at four concentrations: 4.9 μg/g, 8 μg/g, 16 μg/g and 25 μg/g. The effects of DON intake on survival, growth, activity of the antioxidant enzymes - superoxide dismutase (SOD) and catalase (CAT) and the phase II biotransformation enzyme - glutathione-S-transferase (GST), protein content and locomotor activity were monitored in two-month-old T. molitor larvae. The study revealed that DON at concentrations ranging from 4.9 to 25 μg/g wheat bran reduced larval body weight and protein content. Furthermore, it increased SOD and GST activity, had no effect on CAT activity. Also, this study showed that presence of DON in these concentrations has no direct detrimental effects on larval survival, but reduced locomotor activity. The observed effects were particularly pronounced in the larvae given the highest DON concentration 25 μg/g. These finding reveals that DON in artificially contaminated wheat bran at concentrations up to 25 μg/g is able to induce dose-dependent physiological and behavioral changes in T. molitor larvae.",
publisher = "Elsevier Ltd.",
journal = "Journal of Stored Products Research",
title = "Physiological and behavioral effects of the mycotoxin deoxynivalenol in Tenebrio molitor larvae",
volume = "83",
doi = "10.1016/J.JSPR.2019.07.008",
pages = "236-242"
}

Janković Tomanić, M., Petković, B., Todorović, D., Vranković, J.,& Perić Mataruga, V.. (2019). Physiological and behavioral effects of the mycotoxin deoxynivalenol in Tenebrio molitor larvae. in Journal of Stored Products Research
Elsevier Ltd.., 83, 236-242.
https://doi.org/10.1016/J.JSPR.2019.07.008

Janković Tomanić M, Petković B, Todorović D, Vranković J, Perić Mataruga V. Physiological and behavioral effects of the mycotoxin deoxynivalenol in Tenebrio molitor larvae. in Journal of Stored Products Research. 2019;83:236-242.
doi:10.1016/J.JSPR.2019.07.008 .

Janković Tomanić, Milena, Petković, Branka, Todorović, Dajana, Vranković, Jelena, Perić Mataruga, Vesna, "Physiological and behavioral effects of the mycotoxin deoxynivalenol in Tenebrio molitor larvae" in Journal of Stored Products Research, 83 (2019):236-242,
https://doi.org/10.1016/J.JSPR.2019.07.008 . .

TY  - JOUR
AU  - Janković Tomanić, Milena
AU  - Todorović, Dajana
AU  - Stanivuković, Zoran
AU  - Perić Mataruga, Vesna
AU  - Wessjohann, Ludger A.
AU  - Kaluđerović, Goran N.
PY  - 2017
UR  - http://online.journals.tubitak.gov.tr/openDoiPdf.htm?mKodu=biy-1705-76
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2945
AB  - The study presented here aims to elucidate the effects of emodin (EO = 1,3,8-trihydroxy-6-methylanthraquinone) in its free form and when loaded into a mesoporous silica nanocarrier SBA-15 (→ SBA-15|EO) on the activities of the main antioxidative enzymes, superoxide dismutase, catalase, glutathione S-transferase, and glutathione reductase, in larvae of a polyphagous insect pest, the browntail moth Euproctis chrysorrhoea (L.). The results show that only SBA-15|EO upregulates the activities of the tested antioxidative enzymes. These results point to significant differences in the effectiveness of the compound in the free versus the loaded form.
PB  - Scientific and Technical Research Council of Turkey
T2  - Turkish Journal of Biology
T2  - Turkish Journal of Biology
T1  - Mesoporous silica nanoparticles SBA-15 loaded with emodin upregulate the antioxidative defense of Euproctis chrysorrhoea (L.) larvae
IS  - 6
VL  - 41
DO  - 10.3906/biy-1705-76
SP  - 935
EP  - 942
ER  - 

@article{
author = "Janković Tomanić, Milena and Todorović, Dajana and Stanivuković, Zoran and Perić Mataruga, Vesna and Wessjohann, Ludger A. and Kaluđerović, Goran N.",
year = "2017",
abstract = "The study presented here aims to elucidate the effects of emodin (EO = 1,3,8-trihydroxy-6-methylanthraquinone) in its free form and when loaded into a mesoporous silica nanocarrier SBA-15 (→ SBA-15|EO) on the activities of the main antioxidative enzymes, superoxide dismutase, catalase, glutathione S-transferase, and glutathione reductase, in larvae of a polyphagous insect pest, the browntail moth Euproctis chrysorrhoea (L.). The results show that only SBA-15|EO upregulates the activities of the tested antioxidative enzymes. These results point to significant differences in the effectiveness of the compound in the free versus the loaded form.",
publisher = "Scientific and Technical Research Council of Turkey",
journal = "Turkish Journal of Biology, Turkish Journal of Biology",
title = "Mesoporous silica nanoparticles SBA-15 loaded with emodin upregulate the antioxidative defense of Euproctis chrysorrhoea (L.) larvae",
number = "6",
volume = "41",
doi = "10.3906/biy-1705-76",
pages = "935-942"
}

Janković Tomanić, M., Todorović, D., Stanivuković, Z., Perić Mataruga, V., Wessjohann, L. A.,& Kaluđerović, G. N.. (2017). Mesoporous silica nanoparticles SBA-15 loaded with emodin upregulate the antioxidative defense of Euproctis chrysorrhoea (L.) larvae. in Turkish Journal of Biology
Scientific and Technical Research Council of Turkey., 41(6), 935-942.
https://doi.org/10.3906/biy-1705-76

Janković Tomanić M, Todorović D, Stanivuković Z, Perić Mataruga V, Wessjohann LA, Kaluđerović GN. Mesoporous silica nanoparticles SBA-15 loaded with emodin upregulate the antioxidative defense of Euproctis chrysorrhoea (L.) larvae. in Turkish Journal of Biology. 2017;41(6):935-942.
doi:10.3906/biy-1705-76 .

Janković Tomanić, Milena, Todorović, Dajana, Stanivuković, Zoran, Perić Mataruga, Vesna, Wessjohann, Ludger A., Kaluđerović, Goran N., "Mesoporous silica nanoparticles SBA-15 loaded with emodin upregulate the antioxidative defense of Euproctis chrysorrhoea (L.) larvae" in Turkish Journal of Biology, 41, no. 6 (2017):935-942,
https://doi.org/10.3906/biy-1705-76 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Janković Tomanić, Milena
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Milanović, Slobodan
AU  - Stojković, Biljana
PY  - 2017
UR  - http://doi.wiley.com/10.1111/ens.12250
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2924
AB  - The gypsy moth is a generalist insect pest with an extremely wide host range. Adaptive responses of digestive enzymes are important for the successful utilization of plant hosts that differ in the contents and ratios of constituent nutrients and allelochemicals. In the present study, we examined the responses of α-amylase, trypsin, and leucine aminopeptidase to two tree hosts (suitable oak, Quercus cerris, and unsuitable locust tree, Robinia pseudoacacia) in the fourth, fifth, and sixth instars of gypsy moth larvae originating from oak and locust tree forest populations (hereafter assigned as Quercus and Robinia populations, respectively). Gypsy moths from the Robinia forest had been adapting to this unsuitable host for more than 40 generations. To test for population-level host plant specialization, we applied a two-population × two-host experimental design. We compared the levels, developmental patterns, and plasticities of the activities of enzymes. The locust tree diet increased enzyme activity in the fourth instar and reduced activity in advanced instars of the Quercus larvae in comparison to the oak diet. These larvae also exhibited opposite developmental trajectories on the two hosts, i.e. activity increased on the oak diet and decreased on the locust tree diet with the progress of instar. Larvae of the Robinia population were characterized by reduced plasticity of enzyme activity and its developmental trajectories. In addition, elevated trypsin activity in response to an unsuitable host was observed in all instar larvae of the Robinia population, which demonstrated that Robinia larvae had an improved digestive performance than did Quercus larvae.
T2  - Entomological Science
T1  - Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)
IS  - 1
VL  - 20
DO  - 10.1111/ens.12250
SP  - 189
EP  - 194
ER  - 

@article{
author = "Lazarević, Jelica and Janković Tomanić, Milena and Savković, Uroš and Đorđević, Mirko and Milanović, Slobodan and Stojković, Biljana",
year = "2017",
abstract = "The gypsy moth is a generalist insect pest with an extremely wide host range. Adaptive responses of digestive enzymes are important for the successful utilization of plant hosts that differ in the contents and ratios of constituent nutrients and allelochemicals. In the present study, we examined the responses of α-amylase, trypsin, and leucine aminopeptidase to two tree hosts (suitable oak, Quercus cerris, and unsuitable locust tree, Robinia pseudoacacia) in the fourth, fifth, and sixth instars of gypsy moth larvae originating from oak and locust tree forest populations (hereafter assigned as Quercus and Robinia populations, respectively). Gypsy moths from the Robinia forest had been adapting to this unsuitable host for more than 40 generations. To test for population-level host plant specialization, we applied a two-population × two-host experimental design. We compared the levels, developmental patterns, and plasticities of the activities of enzymes. The locust tree diet increased enzyme activity in the fourth instar and reduced activity in advanced instars of the Quercus larvae in comparison to the oak diet. These larvae also exhibited opposite developmental trajectories on the two hosts, i.e. activity increased on the oak diet and decreased on the locust tree diet with the progress of instar. Larvae of the Robinia population were characterized by reduced plasticity of enzyme activity and its developmental trajectories. In addition, elevated trypsin activity in response to an unsuitable host was observed in all instar larvae of the Robinia population, which demonstrated that Robinia larvae had an improved digestive performance than did Quercus larvae.",
journal = "Entomological Science",
title = "Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)",
number = "1",
volume = "20",
doi = "10.1111/ens.12250",
pages = "189-194"
}

Lazarević, J., Janković Tomanić, M., Savković, U., Đorđević, M., Milanović, S.,& Stojković, B.. (2017). Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae). in Entomological Science, 20(1), 189-194.
https://doi.org/10.1111/ens.12250

Lazarević J, Janković Tomanić M, Savković U, Đorđević M, Milanović S, Stojković B. Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae). in Entomological Science. 2017;20(1):189-194.
doi:10.1111/ens.12250 .

Lazarević, Jelica, Janković Tomanić, Milena, Savković, Uroš, Đorđević, Mirko, Milanović, Slobodan, Stojković, Biljana, "Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)" in Entomological Science, 20, no. 1 (2017):189-194,
https://doi.org/10.1111/ens.12250 . .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Janković Tomanić, Milena
AU  - Kostić, Igor
AU  - Kostić, Miroslav
AU  - Morina, Filis
AU  - Živanović, Bojana
AU  - Lazarević, Jelica
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3956
AB  - Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, fre-
quently encounter spatial and temporal variations in diet quality. Such variation favoured the evolu-
tion of high behavioural and physiological plasticity which, depending on forest stand composition,
enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable
habitat, interspecific and intraspecific host quality variation may provoke significant variation in
gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently
explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches
between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.)
(TH and HT groups), under controlled laboratory conditions, and compared larval performance
between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian
oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak
quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hun-
garian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption
rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activ-
ities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT lar-
vae. It appeared that the lower growth in the TH group than in the TT group was caused by both
behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming
oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet
experience influenced the sensitivity of the most examined traits to less suitable Hungarian oaks, sug-
gesting the development of behavioural and physiological adjustments. Our results indicate that
lower risks of defoliation by gypsy moth might be expected in mixed stands with a higher proportion
of Hungarian oak.
PB  - The Netherlands Entomological Society
T2  - Entomologia Experimentalis et Applicata
T1  - Behavioural and physiological plasticity of gypsy moth larvae to host plant switching
IS  - 2
VL  - 158
DO  - 10.1111/eea.12388
SP  - 152
EP  - 162
ER  - 

@article{
author = "Milanović, Slobodan and Janković Tomanić, Milena and Kostić, Igor and Kostić, Miroslav and Morina, Filis and Živanović, Bojana and Lazarević, Jelica",
year = "2016",
abstract = "Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, fre-
quently encounter spatial and temporal variations in diet quality. Such variation favoured the evolu-
tion of high behavioural and physiological plasticity which, depending on forest stand composition,
enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable
habitat, interspecific and intraspecific host quality variation may provoke significant variation in
gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently
explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches
between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.)
(TH and HT groups), under controlled laboratory conditions, and compared larval performance
between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian
oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak
quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hun-
garian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption
rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activ-
ities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT lar-
vae. It appeared that the lower growth in the TH group than in the TT group was caused by both
behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming
oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet
experience influenced the sensitivity of the most examined traits to less suitable Hungarian oaks, sug-
gesting the development of behavioural and physiological adjustments. Our results indicate that
lower risks of defoliation by gypsy moth might be expected in mixed stands with a higher proportion
of Hungarian oak.",
publisher = "The Netherlands Entomological Society",
journal = "Entomologia Experimentalis et Applicata",
title = "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching",
number = "2",
volume = "158",
doi = "10.1111/eea.12388",
pages = "152-162"
}

Milanović, S., Janković Tomanić, M., Kostić, I., Kostić, M., Morina, F., Živanović, B.,& Lazarević, J.. (2016). Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis et Applicata
The Netherlands Entomological Society., 158(2), 152-162.
https://doi.org/10.1111/eea.12388

Milanović S, Janković Tomanić M, Kostić I, Kostić M, Morina F, Živanović B, Lazarević J. Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis et Applicata. 2016;158(2):152-162.
doi:10.1111/eea.12388 .

Milanović, Slobodan, Janković Tomanić, Milena, Kostić, Igor, Kostić, Miroslav, Morina, Filis, Živanović, Bojana, Lazarević, Jelica, "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching" in Entomologia Experimentalis et Applicata, 158, no. 2 (2016):152-162,
https://doi.org/10.1111/eea.12388 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Janković Tomanić, Milena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2346
AB  - Because food proteins are crucial for insect survival, growth, and
   fecundity, enzymes involved in their digestion have attracted the
   attention of fundamental entomologists studying the mechanisms and
   patterns of dietary specialization, and applied entomologists searching
   for more efficient modes of pest control. Most insects digest proteins
   using trypsin, an endopeptidase that cleaves polypeptide chains on the
   carboxyl side of arginine and lysine, two basic amino acids. As the most
   ancestral proteinase, trypsin is wide-spread in the digestive tract of
   insects from various orders and with various feeding habits. The present
   review focuses on biochemical and molecular characteristics, mechanisms
   of regulation, and adaptive/non-adaptive changes of trypsin activity in
   response to heterogeneous food environments in a phylogenetic context.
   Within- and among-species variations in trypsin structure and regulation
   that contribute to better matching with specific food types are
   emphasized. We also discuss the relevance of these data for choosing an
   appropriate strategy for control of insect pests. Pest control
   strategies to reduce trypsin activity by interfering with substrate
   binding or trypsin synthesis and secretion have been suggested.
   Successful application of these procedures requires a multi-disciplinary
   approach and knowledge about digestive physiology of the pest, as well
   as the structural characteristics of trypsins that determine their
   interaction with proteinaceous inhibitors and other food compounds,
   about patterns of developmental changes in trypsin gene expression,
   adaptive responses of insects to food composition, and various
   environmental effects on trypsin activity.
T2  - Entomologia Experimentalis Et Applicata
T1  - Dietary and phylogenetic correlates of digestive trypsin activity in
 insect pests
IS  - 2
VL  - 157
DO  - 10.1111/eea.12349
SP  - 123
EP  - 151
ER  - 

@article{
author = "Lazarević, Jelica and Janković Tomanić, Milena",
year = "2015",
abstract = "Because food proteins are crucial for insect survival, growth, and
   fecundity, enzymes involved in their digestion have attracted the
   attention of fundamental entomologists studying the mechanisms and
   patterns of dietary specialization, and applied entomologists searching
   for more efficient modes of pest control. Most insects digest proteins
   using trypsin, an endopeptidase that cleaves polypeptide chains on the
   carboxyl side of arginine and lysine, two basic amino acids. As the most
   ancestral proteinase, trypsin is wide-spread in the digestive tract of
   insects from various orders and with various feeding habits. The present
   review focuses on biochemical and molecular characteristics, mechanisms
   of regulation, and adaptive/non-adaptive changes of trypsin activity in
   response to heterogeneous food environments in a phylogenetic context.
   Within- and among-species variations in trypsin structure and regulation
   that contribute to better matching with specific food types are
   emphasized. We also discuss the relevance of these data for choosing an
   appropriate strategy for control of insect pests. Pest control
   strategies to reduce trypsin activity by interfering with substrate
   binding or trypsin synthesis and secretion have been suggested.
   Successful application of these procedures requires a multi-disciplinary
   approach and knowledge about digestive physiology of the pest, as well
   as the structural characteristics of trypsins that determine their
   interaction with proteinaceous inhibitors and other food compounds,
   about patterns of developmental changes in trypsin gene expression,
   adaptive responses of insects to food composition, and various
   environmental effects on trypsin activity.",
journal = "Entomologia Experimentalis Et Applicata",
title = "Dietary and phylogenetic correlates of digestive trypsin activity in
 insect pests",
number = "2",
volume = "157",
doi = "10.1111/eea.12349",
pages = "123-151"
}

Lazarević, J.,& Janković Tomanić, M.. (2015). Dietary and phylogenetic correlates of digestive trypsin activity in
 insect pests. in Entomologia Experimentalis Et Applicata, 157(2), 123-151.
https://doi.org/10.1111/eea.12349

Lazarević J, Janković Tomanić M. Dietary and phylogenetic correlates of digestive trypsin activity in
 insect pests. in Entomologia Experimentalis Et Applicata. 2015;157(2):123-151.
doi:10.1111/eea.12349 .

Lazarević, Jelica, Janković Tomanić, Milena, "Dietary and phylogenetic correlates of digestive trypsin activity in
 insect pests" in Entomologia Experimentalis Et Applicata, 157, no. 2 (2015):123-151,
https://doi.org/10.1111/eea.12349 . .

TY  - JOUR
AU  - Janković Tomanić, Milena
AU  - Šešlija Jovanović, Darka
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Lazarević, Jelica
PY  - 2015
UR  - http://www.sciencedirect.com/science/article/pii/S0022474X15000272?via%3Dihub#ack0010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2922
AB  - Bean weevil, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae, Bruchinae) is a cosmopolitan pest of legume stored products. Storages with various legume seeds can facilitate shifts of the weevil from its primary host, common bean (Phaseolus vulgaris L.), to other legume species and enable host expansion, i.e. broadening of the range of acceptable plant-hosts. In the first generation of host shift, survival of an insect depends on ability to adjust its physiology to altered content of nutrients and secondary metabolites in novel host. On a long-term scale, physiological adaptations to a new host can comprise modifications both in level and plasticity of physiological traits. Changes in activity of phosphatases play an essential role in this process due to their involvement in diverse functions. This study investigated alterations in activity of total acid, lysosomal acid and alkaline phosphatases using laboratory populations of A.obtectus which were maintained either on the optimal host (common bean) or on the suboptimal host (chickpea, Cicer arietinum L.) for 28 years. To determine short-term (plastic) effects, subsets of individuals from each population were exposed to the alternative host for one generation. Our results revealed that one-generation shift to chickpea significantly increased phosphatases' activity reflecting immediate plastic response to nutritional/allelochemical stress where these enzymes might be involved in defense mechanisms. On the other hand, both level and plasticity of phosphatases' activities significantly declined as a long-term response to Cicer-based diet suggesting that selection on chickpea favored resistance mechanisms that were less costly than phosphatases. Considering diverse roles of phosphatases we suggest that such modifications could be crucial for expanding host range and might have implications on efficiency of chemical and botanical insecticides.
T2  - Journal of Stored Products Research
T1  - Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say)
VL  - 62
DO  - 10.1016/j.jspr.2015.03.008
SP  - 32
EP  - 35
ER  - 

@article{
author = "Janković Tomanić, Milena and Šešlija Jovanović, Darka and Savković, Uroš and Đorđević, Mirko and Stojković, Biljana and Lazarević, Jelica",
year = "2015",
abstract = "Bean weevil, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae, Bruchinae) is a cosmopolitan pest of legume stored products. Storages with various legume seeds can facilitate shifts of the weevil from its primary host, common bean (Phaseolus vulgaris L.), to other legume species and enable host expansion, i.e. broadening of the range of acceptable plant-hosts. In the first generation of host shift, survival of an insect depends on ability to adjust its physiology to altered content of nutrients and secondary metabolites in novel host. On a long-term scale, physiological adaptations to a new host can comprise modifications both in level and plasticity of physiological traits. Changes in activity of phosphatases play an essential role in this process due to their involvement in diverse functions. This study investigated alterations in activity of total acid, lysosomal acid and alkaline phosphatases using laboratory populations of A.obtectus which were maintained either on the optimal host (common bean) or on the suboptimal host (chickpea, Cicer arietinum L.) for 28 years. To determine short-term (plastic) effects, subsets of individuals from each population were exposed to the alternative host for one generation. Our results revealed that one-generation shift to chickpea significantly increased phosphatases' activity reflecting immediate plastic response to nutritional/allelochemical stress where these enzymes might be involved in defense mechanisms. On the other hand, both level and plasticity of phosphatases' activities significantly declined as a long-term response to Cicer-based diet suggesting that selection on chickpea favored resistance mechanisms that were less costly than phosphatases. Considering diverse roles of phosphatases we suggest that such modifications could be crucial for expanding host range and might have implications on efficiency of chemical and botanical insecticides.",
journal = "Journal of Stored Products Research",
title = "Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say)",
volume = "62",
doi = "10.1016/j.jspr.2015.03.008",
pages = "32-35"
}

Janković Tomanić, M., Šešlija Jovanović, D., Savković, U., Đorđević, M., Stojković, B.,& Lazarević, J.. (2015). Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say). in Journal of Stored Products Research, 62, 32-35.
https://doi.org/10.1016/j.jspr.2015.03.008

Janković Tomanić M, Šešlija Jovanović D, Savković U, Đorđević M, Stojković B, Lazarević J. Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say). in Journal of Stored Products Research. 2015;62:32-35.
doi:10.1016/j.jspr.2015.03.008 .

Janković Tomanić, Milena, Šešlija Jovanović, Darka, Savković, Uroš, Đorđević, Mirko, Stojković, Biljana, Lazarević, Jelica, "Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say)" in Journal of Stored Products Research, 62 (2015):32-35,
https://doi.org/10.1016/j.jspr.2015.03.008 . .

TY  - CONF
AU  - Milanović, Slobodan
AU  - Janković Tomanić, Milena
AU  - Lazarević, Jelica
PY  - 2014
UR  - http://www.worldcat.org/oclc/1123859215
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3979
AB  - Larvae of a generalist species, the gypsy moth (Lymantria dispar), frequently encounter diet heterogeneity due to passive wind-born dispersal of 1st instar as well as host plant switching of advanced instars in mixed stands and during outbreak. Such spatial and temporal variation in nutritional environment favors their exquisite behavioral and physiological plasticity. We studied the effects of switching between Turkey (Quercus cerris) and Hungarian oak (Q. frainetto) on growth, nutritional indices and activities of protease and amylase in gypsy moth larvae. In Serbia, these oak species are often found in mixed stands where outbreaks start. Hungarian oak is less suitable host plant due to lower water and protein content. Switching from turkey to hungarian oak led to prolonged duration of 4th instar, decreased relative growth rate, relative consumption rate and efficiency of conversion of ingested food as well as increased protease and amylase activity. Values of examined traits in gypsy moth larvae switched from hungarian to turkey oak did not differ from values obtained in larvae continuously reared on turkey oak. Studying physiological plasticity of the gypsy moth in response to variation in suitable hosts are important for understanding population dynamics of this invasive species.
PB  - Royal Entomological Society
C3  - 10th European Congress of Entomology, Royal Entomological Society-UK
T1  - Nutritional indices and digestive enzyme activities of gypsy moth larvae switched between turkey and hungarian oak
SP  - 119
EP  - 119
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3979
ER  - 

@conference{
author = "Milanović, Slobodan and Janković Tomanić, Milena and Lazarević, Jelica",
year = "2014",
abstract = "Larvae of a generalist species, the gypsy moth (Lymantria dispar), frequently encounter diet heterogeneity due to passive wind-born dispersal of 1st instar as well as host plant switching of advanced instars in mixed stands and during outbreak. Such spatial and temporal variation in nutritional environment favors their exquisite behavioral and physiological plasticity. We studied the effects of switching between Turkey (Quercus cerris) and Hungarian oak (Q. frainetto) on growth, nutritional indices and activities of protease and amylase in gypsy moth larvae. In Serbia, these oak species are often found in mixed stands where outbreaks start. Hungarian oak is less suitable host plant due to lower water and protein content. Switching from turkey to hungarian oak led to prolonged duration of 4th instar, decreased relative growth rate, relative consumption rate and efficiency of conversion of ingested food as well as increased protease and amylase activity. Values of examined traits in gypsy moth larvae switched from hungarian to turkey oak did not differ from values obtained in larvae continuously reared on turkey oak. Studying physiological plasticity of the gypsy moth in response to variation in suitable hosts are important for understanding population dynamics of this invasive species.",
publisher = "Royal Entomological Society",
journal = "10th European Congress of Entomology, Royal Entomological Society-UK",
title = "Nutritional indices and digestive enzyme activities of gypsy moth larvae switched between turkey and hungarian oak",
pages = "119-119",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3979"
}

Milanović, S., Janković Tomanić, M.,& Lazarević, J.. (2014). Nutritional indices and digestive enzyme activities of gypsy moth larvae switched between turkey and hungarian oak. in 10th European Congress of Entomology, Royal Entomological Society-UK
Royal Entomological Society., 119-119.
https://hdl.handle.net/21.15107/rcub_ibiss_3979

Milanović S, Janković Tomanić M, Lazarević J. Nutritional indices and digestive enzyme activities of gypsy moth larvae switched between turkey and hungarian oak. in 10th European Congress of Entomology, Royal Entomological Society-UK. 2014;:119-119.
https://hdl.handle.net/21.15107/rcub_ibiss_3979 .

Milanović, Slobodan, Janković Tomanić, Milena, Lazarević, Jelica, "Nutritional indices and digestive enzyme activities of gypsy moth larvae switched between turkey and hungarian oak" in 10th European Congress of Entomology, Royal Entomological Society-UK (2014):119-119,
https://hdl.handle.net/21.15107/rcub_ibiss_3979 .

TY  - JOUR
AU  - Milanovic, Slobodan
AU  - Mihajlovic, Ljubodrag
AU  - Karadzic, Dragan
AU  - Jankovsky, Libor
AU  - Aleksic, Predrag
AU  - Janković Tomanić, Milena
AU  - Lazarević, Jelica
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2265
AB  - Gypsy moths and powdery mildew play a significant role in oak decline
   processes. However, information is lacking on the effects on the gypsy
   moth of impaired tree vitality caused by defoliation or parasite
   infection. We assessed how pedunculate oak leaves collected from
   vigorous, declining, and infected trees influenced gypsy moth preference
   and performance (growth and nutritional indices). We found a negative
   effect of powdery mildew-infected leaves on gypsy moth performance,
   while declining trees had positive effects on gypsy moth performance and
   preference. All examined parameters of larvae fed declining oak leaves
   were higher than those of larvae fed vigorous oak leaves. Increased
   growth on declining oak leaves was caused by both higher consumption and
   more efficient food utilization. The results of this research could help
   us to better understand multitrophic interactions in complex communities
   such as oak forests.
T2  - Archives of Biological Sciences
T1  - Effects of pedunculate oak tree vitality on gypsy moth preference and performance
IS  - 4
VL  - 66
DO  - 10.2298/ABS1404659M
SP  - 1659
EP  - 1672
ER  - 

@article{
author = "Milanovic, Slobodan and Mihajlovic, Ljubodrag and Karadzic, Dragan and Jankovsky, Libor and Aleksic, Predrag and Janković Tomanić, Milena and Lazarević, Jelica",
year = "2014",
abstract = "Gypsy moths and powdery mildew play a significant role in oak decline
   processes. However, information is lacking on the effects on the gypsy
   moth of impaired tree vitality caused by defoliation or parasite
   infection. We assessed how pedunculate oak leaves collected from
   vigorous, declining, and infected trees influenced gypsy moth preference
   and performance (growth and nutritional indices). We found a negative
   effect of powdery mildew-infected leaves on gypsy moth performance,
   while declining trees had positive effects on gypsy moth performance and
   preference. All examined parameters of larvae fed declining oak leaves
   were higher than those of larvae fed vigorous oak leaves. Increased
   growth on declining oak leaves was caused by both higher consumption and
   more efficient food utilization. The results of this research could help
   us to better understand multitrophic interactions in complex communities
   such as oak forests.",
journal = "Archives of Biological Sciences",
title = "Effects of pedunculate oak tree vitality on gypsy moth preference and performance",
number = "4",
volume = "66",
doi = "10.2298/ABS1404659M",
pages = "1659-1672"
}

Milanovic, S., Mihajlovic, L., Karadzic, D., Jankovsky, L., Aleksic, P., Janković Tomanić, M.,& Lazarević, J.. (2014). Effects of pedunculate oak tree vitality on gypsy moth preference and performance. in Archives of Biological Sciences, 66(4), 1659-1672.
https://doi.org/10.2298/ABS1404659M

Milanovic S, Mihajlovic L, Karadzic D, Jankovsky L, Aleksic P, Janković Tomanić M, Lazarević J. Effects of pedunculate oak tree vitality on gypsy moth preference and performance. in Archives of Biological Sciences. 2014;66(4):1659-1672.
doi:10.2298/ABS1404659M .

Milanovic, Slobodan, Mihajlovic, Ljubodrag, Karadzic, Dragan, Jankovsky, Libor, Aleksic, Predrag, Janković Tomanić, Milena, Lazarević, Jelica, "Effects of pedunculate oak tree vitality on gypsy moth preference and performance" in Archives of Biological Sciences, 66, no. 4 (2014):1659-1672,
https://doi.org/10.2298/ABS1404659M . .

TY  - JOUR
AU  - Mrdaković, Marija
AU  - Perić Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Janković Tomanić, Milena
AU  - Mirčić, Dejan Lj
AU  - Lazarević, Jelica
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1057
AB  - The effects of tannic acid on mean values and genetic variation in fitness-related traits (mass, relative growth rate) and specific activities of digestive enzymes (total proteases, alpha-glucosidase and lipase), and genetic variation in their plasticity, were investigated in fifth instar larvae of Lymantria dispar L. (Lepidoptera: Lymantriidae) originating from two populations with different host use histories (oak and locust-tree). The two populations did not differentiate with respect to fitness-related traits, i.e. adverse effects of tannic acid were similar in both populations. However, Robinia larvae, which originated from the locust-tree forest, were characterized by higher total protease and lipase activity and lower alpha-glucosidase activity than Quercus larvae, which originated from the oak forest. Higher plasticity of lipase and lower plasticity of alpha-glucosidase in response to tannic acid were also recorded. Quantitative genetic analysis revealed mostly significant expression of genetic variation in the examined traits and trait plasticity, suggesting the potential for evolution of adaptive plastic responses to new environmental conditions and presence of a stressor. The genetic correlations observed between the environments significantly differed from "one", which indicates there are no constraints on the evolution of trait plasticity.
PB  - Czech Academy of Sciences
T2  - European Journal of Entomology
T1  - Response of Lymantria dispar (Lepidoptera: Lymantriidae) larvae from differently adapted populations to allelochemical stress: Effects of tannic acid
IS  - 1
VL  - 110
DO  - 10.14411/eje.2013.007
SP  - 55
EP  - 63
ER  - 

@article{
author = "Mrdaković, Marija and Perić Mataruga, Vesna and Ilijin, Larisa and Vlahović, Milena and Janković Tomanić, Milena and Mirčić, Dejan Lj and Lazarević, Jelica",
year = "2013",
abstract = "The effects of tannic acid on mean values and genetic variation in fitness-related traits (mass, relative growth rate) and specific activities of digestive enzymes (total proteases, alpha-glucosidase and lipase), and genetic variation in their plasticity, were investigated in fifth instar larvae of Lymantria dispar L. (Lepidoptera: Lymantriidae) originating from two populations with different host use histories (oak and locust-tree). The two populations did not differentiate with respect to fitness-related traits, i.e. adverse effects of tannic acid were similar in both populations. However, Robinia larvae, which originated from the locust-tree forest, were characterized by higher total protease and lipase activity and lower alpha-glucosidase activity than Quercus larvae, which originated from the oak forest. Higher plasticity of lipase and lower plasticity of alpha-glucosidase in response to tannic acid were also recorded. Quantitative genetic analysis revealed mostly significant expression of genetic variation in the examined traits and trait plasticity, suggesting the potential for evolution of adaptive plastic responses to new environmental conditions and presence of a stressor. The genetic correlations observed between the environments significantly differed from "one", which indicates there are no constraints on the evolution of trait plasticity.",
publisher = "Czech Academy of Sciences",
journal = "European Journal of Entomology",
title = "Response of Lymantria dispar (Lepidoptera: Lymantriidae) larvae from differently adapted populations to allelochemical stress: Effects of tannic acid",
number = "1",
volume = "110",
doi = "10.14411/eje.2013.007",
pages = "55-63"
}

Mrdaković, M., Perić Mataruga, V., Ilijin, L., Vlahović, M., Janković Tomanić, M., Mirčić, D. L.,& Lazarević, J.. (2013). Response of Lymantria dispar (Lepidoptera: Lymantriidae) larvae from differently adapted populations to allelochemical stress: Effects of tannic acid. in European Journal of Entomology
Czech Academy of Sciences., 110(1), 55-63.
https://doi.org/10.14411/eje.2013.007

Mrdaković M, Perić Mataruga V, Ilijin L, Vlahović M, Janković Tomanić M, Mirčić DL, Lazarević J. Response of Lymantria dispar (Lepidoptera: Lymantriidae) larvae from differently adapted populations to allelochemical stress: Effects of tannic acid. in European Journal of Entomology. 2013;110(1):55-63.
doi:10.14411/eje.2013.007 .

Mrdaković, Marija, Perić Mataruga, Vesna, Ilijin, Larisa, Vlahović, Milena, Janković Tomanić, Milena, Mirčić, Dejan Lj, Lazarević, Jelica, "Response of Lymantria dispar (Lepidoptera: Lymantriidae) larvae from differently adapted populations to allelochemical stress: Effects of tannic acid" in European Journal of Entomology, 110, no. 1 (2013):55-63,
https://doi.org/10.14411/eje.2013.007 . .

TY  - THES
AU  - Janković Tomanić, Milena
PY  - 2012
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=416
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:6140/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=1024555186
UR  - http://nardus.mpn.gov.rs/123456789/2094
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2389
AB  - Temperatura i kvalitet hrane utiču na performansu larvi gubara, Lymantria
dispar L. nezavisno ili u međusobnoj interakciji. Strategije preživljavanja larvi
gubara u temperaturno kao i nutritivno heterogenoj sredini obuhvataju različite
tipove reverzibilne i ireverzibilne fenotipske plastičnosti, koje preko uticaja na
usvajanje i raspodelu resursa utiču na osobine životne istorije i rezistentnost
prema ekstremnim uslovima životne sredine.
U cilju ispitivanja efekata temperature i kvaliteta hrane, tj. sadržaja proteina i
ugljenih hidrata u hrani na komponente adaptivne vrednosti, kao i ekspresiju
genetičke varijabilnosti, larve gubara su izložene delovanju tri različite
temperature (suboptimalna, optimalna i supraoptimalna) i 4 kombinacije
hranljivog sastava dijete, koje su se međusobno razlikovale kako u ukupnom
sadržaju proteina i ugljenih hidrata, tako i u njihovom međusobnom odnosu. U
istim eksperimentalnim uslovima ispitivana je uloga procesa varenja, odnosno
aktivnosti digestivnih enzima u usklađivanju odnosa i količine unetih nutienata sa
potrebama organizma na različitim temperaturama. Takođe, ispitan je uticaj
nutritivne vrednosti i balansiranosti hrane na senzitivnost gubara prema stresnim
temperaturama.
Nepovoljne temperature i nizak sadržaj proteina u hrani, kao i disbalans
proteina u odnosu na ugljene hidrate, smanjuju performansu larvi gubara. Uticaji
temperature i kvaliteta hrane na komponente adaptivne vrednosti: preživljavanje,
trajanje razvića, masu i relativnu brzinu rasta, uglavnom su međusobno nezavisni.
Pokazano je da povišena temperatura smanjuje preživljavanje i trajanje razvića
larvi ali dovodi do povećanja relativne brzine rasta. Nutritivni sastav hrane nije
uticao na preživljavanje, ali je nizak sadržaj proteina u hrani dovodio do
produžavanja razvića, smanjenja mase i relativne brzine rasta larvi. Relativna
brzina rasta larvi je bila manja i pri visokom sadržaju ugljenih hidrata u hrani, dok
je smanjenje mase larvi na hrani sa niskim sadržajem proteina bilo veće ako je i
sadržaj ugljenih hidrata bio nizak.
Ako se larve hrane dijetom sa visokim sadržajem proteina dolazi do smanjenja
aktivnosti proteolitičkih enzima, ukupnih proteaza i tripsina, dok se u uslovima
niskog sadžaja proteina i visokog sadržaja ugljenih hidrata smanjuje aktivnost
karbohidraza, α-amilaze i α-glikozidaze. Temperatura i hrana deluju nezavisno na
aktivnost elastaze i tripsina, α-glikozidaze i kiselih fosfataza, dok je za aktivnost
ukupnih proteaza, leucin aminopeptidaze, α-amilaze, lipaze i alkalne fosfataze
pokazana značajna interakcija temperature i nutritivnog sastava dijete. Promena
aktivnosti ukupnih proteaza sa porastom temperature sastoji se u povećanju
aktivnosti na nutritivno najsiromašnijoj i smanjivanju aktivnosti na nutritivno
najbogatijoj hrani, dok se promena aktivnosti α-amilaze u odgovoru na povećanje
temperature sastoji u povećanju aktivnosti na hrani sa niskim sadržajem ugljenih
hidrata. Odgovor lipaze i kiselih fosfataza na nizak sadržaj proteina i visok sadržaj
ugljenih hidrata u hrani, kao i nutritivno siromašnu hranu sastoji se u povećanju
njihove aktivnosti, dok se aktivnost alkalne fosfataze sa porastom temperature
povećava i na hrani sa niskim sadržajem proteina i na hrani sa niskim sadržajem
ugljenih hidrata.
U odgovoru na delovanje temperature, pokazan je „obrnut obrazac“ promene
aktivnosti između endo i egzopeptidaze (elastaze i leucin aminopeptidaze), između
endo i egzokarbohidraze (-amilaze i -glikozidaze) i između alkalne i kiselih
fosfataza, kao i promena fenotipskih korelacija između pojedinih klasa enzima na
nepovoljnim temperaturama i suboptimalnom nutritivnom sadržaju hrane.
Uticaj temperature i kvaliteta hrane uočava se i na nivou promene ekspresije
genetičke varijabilnosti trajanja razvića, kao i preko značajne varijabilnosti
fenotipske plastičnosti u odgovoru na delovanje temperature. Na optimalnoj
temperaturi dolazi do porasta heritabilnosti mase larvi na nutritivno
najsiromašnijoj hrani, dok porast temperature smanjuje heritabilnost mase ako je
u hrani nizak sadržaj jednog ili oba nutrijenta. Genetičke korelacije izmedju
trajanja razvića i mase larvi u nepovoljnim uslovima životne sredine su negativne,
tj. larve koje karakteriše duže larveno razviće istovremeno imaju i manju masu,
dok u optimalnim uslovima nisu detektovane značajne korelacije između ovih
osobina. Većina genetičkih korelacije između sredina, kako za trajanje razvića, tako
i za masu larvi bila je pozitivna, što je očekivan rezultat za vrste generaliste. Sve
genetičke korelacije između sredina za trajanje razvića larvi su pozitivne i nisu
značajno različite od „1” tako da predstavljaju ograničenje za evoluciju fenotipske
plastičnosti. Genetičke korelacije između sredina za masu larvi su pozitivne i
značajno različite od „1” i, mada za masu larvi nije ustanovljena varijabilnost
fenotipske plastičnosti, evolucija plastičnosti je moguća usled značajnih razlika u
heritabilnosti između sredina.
Larve gubara mogu „profitirati“ u nepovoljnim uslovima nutritivno siromašne
hrane, jer je rezistentost gubara na temperaturni stres procenjena na osnovu
vremena preživljavanja, pokazala najveću vrednost u takvim uslovima. Nasuprot
tome, ograničavajući faktori preživljavanja su prethodna aklimacija na
(konstantno) povišenu temperaturu tokom ranih stupnjeva larvenog razvića i
visok sadržaj proteina i ugljenih hidrata u hrani. Suboptimalna temperatura
gajenja i hrana koju karakteriše najmanji odnos proteina u odnosu na ugljene
hidrate, kao i povećanje temperature gajenja na nutritivno najsiromašnijoj hrani,
značajno smanjuju sposobnost larvi gubara da se presvlače na stresnoj
temperaturi.
AB  - Temperature and food quality affect the performance of gypsy moth larvae
Lymantria dispar L. independently or in an interaction with each other. Survival
strategies of gypsy moth larvae in temperature and nutritionally heterogeneous
environments include various types of reversible and irreversible phenotypic
plasticity, which due to the effect of uptake and distribution of resources affect the
life-history traits and resistance to extreme environmental conditions.
In order to investigate the direct and interactive effects of temperature and
food quality on fitness components, as well as the expression of genetic variation,
gypsy moth larvae were exposed to three different temperatures (suboptimal,
optimal and supraoptimal) and 4 sets of nutrient composition of the diet, which
differed in protein and carbohydrate content. Under the same experimental
conditions, the role of digestion and digestive enzyme activity in adjusting nutrient
quantity and ratio with organism needs at different temperatures was
investigated. Also, it was investigated the effect of nutritional value of the food on
sensitivity of gypsy moth larvae to stressful temperatures.
An adverse temperature and low protein content in food, as well as an
imbalance of protein compared to carbohydrates, reduced performance of gypsy
moth larvae. Effects of temperature and food quality on fitness components -
survival, developmental time, larval weight and relative growth rate were mainly
independent. It has been shown that elevated temperature reduces survival and
duration of development, but leads to an increase of the relative growth rate.
Nutritional composition of food had no effect on survival, but the low protein
content led to prolonged developmental time, reduced larval weight and relative
growth rate of gypsy moth larvae. The relative growth rate of larvae was lower if
carbohydrate content in food was high, while larval weight reduction was greater
if protein content was low and the carbohydrate content was high.
Diet with high protein content led to the decrease in specific activities of total
protease and trypsin, while low protein and high carbohydrate diet decreased
specific activities of carbohydrases, α-amylase and α-glucosidase of larvae.
Temperature and food independently influenced activity of elastase and trypsin, α-
glucosidase and acid phosphatase, while total protease, leucine aminopeptidase,
lipase and alkaline phosphatase activities were significantly affected by interaction
of food and temperature. Change of total protease activity with increasing
temperature consisted in its increased activity on nutritionally poorest and
reduced activity in the richest food, whereas the changes of α-amylase activity in
response to increasing temperatures consisted in increased activities in low
carbohydrates food conditions. Responses of lipase and acid phosphatase to low
protein and high carbohydrate content in food, i.e. nutritionally poor food, were an
increase in their activities, while alkaline phosphatase activity increased with
increasing temperature on food that are low in both protein and carbohydrate
content.
In response to temperature, “reverse pattern" of activity was demonstrated
between the endo and exopeptidase (elastase and leucine aminopeptidase), endo
and exocarbohydrase (α-amylase and α-glucosidase) and among alkaline and acid
phosphatases, as well as changes in phenotypic correlations between certain
classes of digestive enzymes to adverse temperatures and suboptimal nutritional
content of food.
The effects of temperature and food quality were also noticeable at the level of
expression of the genetic variability of developmental time, as well as by
significant variability of phenotypic plasticity in response to temperature. At the
optimal temperature there was an increase of heritability of larval weight on
nutritionally poorest food, while rise in temperature decreased heritability of
larval weight if the food was low in one or both nutrients. Within environments
genetic correlations for developmental time and larval weight in adverse
environmental conditions are negative, i.e. larvae, which were characterized by
longer larval development had lower larval weight but, in optimal conditions did
not reveal any significant correlation between these traits. Majority of the acrossenvironment
genetic correlations both for developmental time and larval weight
were positive, which was an expected result for generalist species. Acrossenvironments
genetic correlations for developmental time were positive and not
significantly different from “one”, which represented a constraint for the evolution
of optimal phenotypic plasticity. Across-environments genetic correlations for
larval weight were positive and significantly different from “1” and although, for
larval weight was not found variability of phenotypic plasticity, evolution of
plasticity was possible due to the significant difference in heritability between
environments.
The larvae of gypsy moth ”can benefit” from adverse conditions of nutritionally
poor food, because the gypsy moth resistance to temperature stress, which was
estimated based on survival time, showed the highest value particularly in such
conditions. In contrast, the limiting factor for survival was the previous acclimation
to (constant) elevated temperature during early larval stages and high content of
protein and carbohydrates in food. Suboptimal temperature and food with the
lowest ratio of proteins compared to carbohydrates, as well as an increase in
temperature on nutrient-poorest food, significantly reduced the ability of gypsy
moth larvae to molt in stressful temperatures.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Uticaj temperature i kvaliteta hrane na varijabilnost komponenti adaptivne vrednosti i fiziologiju varenja larvi gubara Lymantria dispar L.
T1  - Effect of temperature and food quality on variability of fitness components and physiology of digestion in the gypsy moth larvae Lymantria dispar L.
SP  - 1
EP  - 231
UR  - https://hdl.handle.net/21.15107/rcub_nardus_2094
ER  - 

@phdthesis{
author = "Janković Tomanić, Milena",
year = "2012",
abstract = "Temperatura i kvalitet hrane utiču na performansu larvi gubara, Lymantria
dispar L. nezavisno ili u međusobnoj interakciji. Strategije preživljavanja larvi
gubara u temperaturno kao i nutritivno heterogenoj sredini obuhvataju različite
tipove reverzibilne i ireverzibilne fenotipske plastičnosti, koje preko uticaja na
usvajanje i raspodelu resursa utiču na osobine životne istorije i rezistentnost
prema ekstremnim uslovima životne sredine.
U cilju ispitivanja efekata temperature i kvaliteta hrane, tj. sadržaja proteina i
ugljenih hidrata u hrani na komponente adaptivne vrednosti, kao i ekspresiju
genetičke varijabilnosti, larve gubara su izložene delovanju tri različite
temperature (suboptimalna, optimalna i supraoptimalna) i 4 kombinacije
hranljivog sastava dijete, koje su se međusobno razlikovale kako u ukupnom
sadržaju proteina i ugljenih hidrata, tako i u njihovom međusobnom odnosu. U
istim eksperimentalnim uslovima ispitivana je uloga procesa varenja, odnosno
aktivnosti digestivnih enzima u usklađivanju odnosa i količine unetih nutienata sa
potrebama organizma na različitim temperaturama. Takođe, ispitan je uticaj
nutritivne vrednosti i balansiranosti hrane na senzitivnost gubara prema stresnim
temperaturama.
Nepovoljne temperature i nizak sadržaj proteina u hrani, kao i disbalans
proteina u odnosu na ugljene hidrate, smanjuju performansu larvi gubara. Uticaji
temperature i kvaliteta hrane na komponente adaptivne vrednosti: preživljavanje,
trajanje razvića, masu i relativnu brzinu rasta, uglavnom su međusobno nezavisni.
Pokazano je da povišena temperatura smanjuje preživljavanje i trajanje razvića
larvi ali dovodi do povećanja relativne brzine rasta. Nutritivni sastav hrane nije
uticao na preživljavanje, ali je nizak sadržaj proteina u hrani dovodio do
produžavanja razvića, smanjenja mase i relativne brzine rasta larvi. Relativna
brzina rasta larvi je bila manja i pri visokom sadržaju ugljenih hidrata u hrani, dok
je smanjenje mase larvi na hrani sa niskim sadržajem proteina bilo veće ako je i
sadržaj ugljenih hidrata bio nizak.
Ako se larve hrane dijetom sa visokim sadržajem proteina dolazi do smanjenja
aktivnosti proteolitičkih enzima, ukupnih proteaza i tripsina, dok se u uslovima
niskog sadžaja proteina i visokog sadržaja ugljenih hidrata smanjuje aktivnost
karbohidraza, α-amilaze i α-glikozidaze. Temperatura i hrana deluju nezavisno na
aktivnost elastaze i tripsina, α-glikozidaze i kiselih fosfataza, dok je za aktivnost
ukupnih proteaza, leucin aminopeptidaze, α-amilaze, lipaze i alkalne fosfataze
pokazana značajna interakcija temperature i nutritivnog sastava dijete. Promena
aktivnosti ukupnih proteaza sa porastom temperature sastoji se u povećanju
aktivnosti na nutritivno najsiromašnijoj i smanjivanju aktivnosti na nutritivno
najbogatijoj hrani, dok se promena aktivnosti α-amilaze u odgovoru na povećanje
temperature sastoji u povećanju aktivnosti na hrani sa niskim sadržajem ugljenih
hidrata. Odgovor lipaze i kiselih fosfataza na nizak sadržaj proteina i visok sadržaj
ugljenih hidrata u hrani, kao i nutritivno siromašnu hranu sastoji se u povećanju
njihove aktivnosti, dok se aktivnost alkalne fosfataze sa porastom temperature
povećava i na hrani sa niskim sadržajem proteina i na hrani sa niskim sadržajem
ugljenih hidrata.
U odgovoru na delovanje temperature, pokazan je „obrnut obrazac“ promene
aktivnosti između endo i egzopeptidaze (elastaze i leucin aminopeptidaze), između
endo i egzokarbohidraze (-amilaze i -glikozidaze) i između alkalne i kiselih
fosfataza, kao i promena fenotipskih korelacija između pojedinih klasa enzima na
nepovoljnim temperaturama i suboptimalnom nutritivnom sadržaju hrane.
Uticaj temperature i kvaliteta hrane uočava se i na nivou promene ekspresije
genetičke varijabilnosti trajanja razvića, kao i preko značajne varijabilnosti
fenotipske plastičnosti u odgovoru na delovanje temperature. Na optimalnoj
temperaturi dolazi do porasta heritabilnosti mase larvi na nutritivno
najsiromašnijoj hrani, dok porast temperature smanjuje heritabilnost mase ako je
u hrani nizak sadržaj jednog ili oba nutrijenta. Genetičke korelacije izmedju
trajanja razvića i mase larvi u nepovoljnim uslovima životne sredine su negativne,
tj. larve koje karakteriše duže larveno razviće istovremeno imaju i manju masu,
dok u optimalnim uslovima nisu detektovane značajne korelacije između ovih
osobina. Većina genetičkih korelacije između sredina, kako za trajanje razvića, tako
i za masu larvi bila je pozitivna, što je očekivan rezultat za vrste generaliste. Sve
genetičke korelacije između sredina za trajanje razvića larvi su pozitivne i nisu
značajno različite od „1” tako da predstavljaju ograničenje za evoluciju fenotipske
plastičnosti. Genetičke korelacije između sredina za masu larvi su pozitivne i
značajno različite od „1” i, mada za masu larvi nije ustanovljena varijabilnost
fenotipske plastičnosti, evolucija plastičnosti je moguća usled značajnih razlika u
heritabilnosti između sredina.
Larve gubara mogu „profitirati“ u nepovoljnim uslovima nutritivno siromašne
hrane, jer je rezistentost gubara na temperaturni stres procenjena na osnovu
vremena preživljavanja, pokazala najveću vrednost u takvim uslovima. Nasuprot
tome, ograničavajući faktori preživljavanja su prethodna aklimacija na
(konstantno) povišenu temperaturu tokom ranih stupnjeva larvenog razvića i
visok sadržaj proteina i ugljenih hidrata u hrani. Suboptimalna temperatura
gajenja i hrana koju karakteriše najmanji odnos proteina u odnosu na ugljene
hidrate, kao i povećanje temperature gajenja na nutritivno najsiromašnijoj hrani,
značajno smanjuju sposobnost larvi gubara da se presvlače na stresnoj
temperaturi., Temperature and food quality affect the performance of gypsy moth larvae
Lymantria dispar L. independently or in an interaction with each other. Survival
strategies of gypsy moth larvae in temperature and nutritionally heterogeneous
environments include various types of reversible and irreversible phenotypic
plasticity, which due to the effect of uptake and distribution of resources affect the
life-history traits and resistance to extreme environmental conditions.
In order to investigate the direct and interactive effects of temperature and
food quality on fitness components, as well as the expression of genetic variation,
gypsy moth larvae were exposed to three different temperatures (suboptimal,
optimal and supraoptimal) and 4 sets of nutrient composition of the diet, which
differed in protein and carbohydrate content. Under the same experimental
conditions, the role of digestion and digestive enzyme activity in adjusting nutrient
quantity and ratio with organism needs at different temperatures was
investigated. Also, it was investigated the effect of nutritional value of the food on
sensitivity of gypsy moth larvae to stressful temperatures.
An adverse temperature and low protein content in food, as well as an
imbalance of protein compared to carbohydrates, reduced performance of gypsy
moth larvae. Effects of temperature and food quality on fitness components -
survival, developmental time, larval weight and relative growth rate were mainly
independent. It has been shown that elevated temperature reduces survival and
duration of development, but leads to an increase of the relative growth rate.
Nutritional composition of food had no effect on survival, but the low protein
content led to prolonged developmental time, reduced larval weight and relative
growth rate of gypsy moth larvae. The relative growth rate of larvae was lower if
carbohydrate content in food was high, while larval weight reduction was greater
if protein content was low and the carbohydrate content was high.
Diet with high protein content led to the decrease in specific activities of total
protease and trypsin, while low protein and high carbohydrate diet decreased
specific activities of carbohydrases, α-amylase and α-glucosidase of larvae.
Temperature and food independently influenced activity of elastase and trypsin, α-
glucosidase and acid phosphatase, while total protease, leucine aminopeptidase,
lipase and alkaline phosphatase activities were significantly affected by interaction
of food and temperature. Change of total protease activity with increasing
temperature consisted in its increased activity on nutritionally poorest and
reduced activity in the richest food, whereas the changes of α-amylase activity in
response to increasing temperatures consisted in increased activities in low
carbohydrates food conditions. Responses of lipase and acid phosphatase to low
protein and high carbohydrate content in food, i.e. nutritionally poor food, were an
increase in their activities, while alkaline phosphatase activity increased with
increasing temperature on food that are low in both protein and carbohydrate
content.
In response to temperature, “reverse pattern" of activity was demonstrated
between the endo and exopeptidase (elastase and leucine aminopeptidase), endo
and exocarbohydrase (α-amylase and α-glucosidase) and among alkaline and acid
phosphatases, as well as changes in phenotypic correlations between certain
classes of digestive enzymes to adverse temperatures and suboptimal nutritional
content of food.
The effects of temperature and food quality were also noticeable at the level of
expression of the genetic variability of developmental time, as well as by
significant variability of phenotypic plasticity in response to temperature. At the
optimal temperature there was an increase of heritability of larval weight on
nutritionally poorest food, while rise in temperature decreased heritability of
larval weight if the food was low in one or both nutrients. Within environments
genetic correlations for developmental time and larval weight in adverse
environmental conditions are negative, i.e. larvae, which were characterized by
longer larval development had lower larval weight but, in optimal conditions did
not reveal any significant correlation between these traits. Majority of the acrossenvironment
genetic correlations both for developmental time and larval weight
were positive, which was an expected result for generalist species. Acrossenvironments
genetic correlations for developmental time were positive and not
significantly different from “one”, which represented a constraint for the evolution
of optimal phenotypic plasticity. Across-environments genetic correlations for
larval weight were positive and significantly different from “1” and although, for
larval weight was not found variability of phenotypic plasticity, evolution of
plasticity was possible due to the significant difference in heritability between
environments.
The larvae of gypsy moth ”can benefit” from adverse conditions of nutritionally
poor food, because the gypsy moth resistance to temperature stress, which was
estimated based on survival time, showed the highest value particularly in such
conditions. In contrast, the limiting factor for survival was the previous acclimation
to (constant) elevated temperature during early larval stages and high content of
protein and carbohydrates in food. Suboptimal temperature and food with the
lowest ratio of proteins compared to carbohydrates, as well as an increase in
temperature on nutrient-poorest food, significantly reduced the ability of gypsy
moth larvae to molt in stressful temperatures.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Uticaj temperature i kvaliteta hrane na varijabilnost komponenti adaptivne vrednosti i fiziologiju varenja larvi gubara Lymantria dispar L., Effect of temperature and food quality on variability of fitness components and physiology of digestion in the gypsy moth larvae Lymantria dispar L.",
pages = "1-231",
url = "https://hdl.handle.net/21.15107/rcub_nardus_2094"
}

Janković Tomanić, M.. (2012). Uticaj temperature i kvaliteta hrane na varijabilnost komponenti adaptivne vrednosti i fiziologiju varenja larvi gubara Lymantria dispar L.. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-231.
https://hdl.handle.net/21.15107/rcub_nardus_2094

Janković Tomanić M. Uticaj temperature i kvaliteta hrane na varijabilnost komponenti adaptivne vrednosti i fiziologiju varenja larvi gubara Lymantria dispar L.. in University of Belgrade, Faculty of Biology. 2012;:1-231.
https://hdl.handle.net/21.15107/rcub_nardus_2094 .

Janković Tomanić, Milena, "Uticaj temperature i kvaliteta hrane na varijabilnost komponenti adaptivne vrednosti i fiziologiju varenja larvi gubara Lymantria dispar L." in University of Belgrade, Faculty of Biology (2012):1-231,
https://hdl.handle.net/21.15107/rcub_nardus_2094 .

TY  - JOUR
AU  - Janković Tomanić, Milena
AU  - Lazarević, Jelica
PY  - 2012
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/314
AB  - To assess the plastic and genetic components of variation in responses of gypsy moth (Lymantria dispar) 4th instar larvae to temperature and food quality, we applied a split-family four-environment experimental design where full-sibs were reared on two constant temperatures (23°C and 28°C) and two concentrations of dietary nitrogen (1.5 and 3.7% dry weight). A temperature of 28°C and low dietary nitrogen decreased larval weight and prolonged larval developmental time, while viability was not affected. Only a marginally significant interaction between the two environmental factors was found for larval weight. The broad-sense heritability for larval developmental time did not change across environments, and across-environment genetic correlations were close to one. Heritability for larval weight depended on environmental and across-environmental genetic correlations that were not significant. There was no evidence of a trade-off between developmental time and larval weight. The implications of the obtained results for the evolution of phenotypic plasticity in complex environments are discussed.
T2  - Archives of Biological Sciences
T1  - Effects of temperature and dietary nitrogen on genetic variation and covariation in gypsy moth larval performance traits
IS  - 3
VL  - 64
SP  - 1109
EP  - 1116
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_314
ER  - 

@article{
author = "Janković Tomanić, Milena and Lazarević, Jelica",
year = "2012, 2012",
abstract = "To assess the plastic and genetic components of variation in responses of gypsy moth (Lymantria dispar) 4th instar larvae to temperature and food quality, we applied a split-family four-environment experimental design where full-sibs were reared on two constant temperatures (23°C and 28°C) and two concentrations of dietary nitrogen (1.5 and 3.7% dry weight). A temperature of 28°C and low dietary nitrogen decreased larval weight and prolonged larval developmental time, while viability was not affected. Only a marginally significant interaction between the two environmental factors was found for larval weight. The broad-sense heritability for larval developmental time did not change across environments, and across-environment genetic correlations were close to one. Heritability for larval weight depended on environmental and across-environmental genetic correlations that were not significant. There was no evidence of a trade-off between developmental time and larval weight. The implications of the obtained results for the evolution of phenotypic plasticity in complex environments are discussed.",
journal = "Archives of Biological Sciences",
title = "Effects of temperature and dietary nitrogen on genetic variation and covariation in gypsy moth larval performance traits",
number = "3",
volume = "64",
pages = "1109-1116",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_314"
}

Janković Tomanić, M.,& Lazarević, J.. (2012). Effects of temperature and dietary nitrogen on genetic variation and covariation in gypsy moth larval performance traits. in Archives of Biological Sciences, 64(3), 1109-1116.
https://hdl.handle.net/21.15107/rcub_ibiss_314

Janković Tomanić M, Lazarević J. Effects of temperature and dietary nitrogen on genetic variation and covariation in gypsy moth larval performance traits. in Archives of Biological Sciences. 2012;64(3):1109-1116.
https://hdl.handle.net/21.15107/rcub_ibiss_314 .

Janković Tomanić, Milena, Lazarević, Jelica, "Effects of temperature and dietary nitrogen on genetic variation and covariation in gypsy moth larval performance traits" in Archives of Biological Sciences, 64, no. 3 (2012):1109-1116,
https://hdl.handle.net/21.15107/rcub_ibiss_314 .

TY  - JOUR
AU  - Perić Mataruga, Vesna
AU  - Vlahović, Milena
AU  - Petković, Branka
AU  - Ilijin, Larisa
AU  - Janković Tomanić, Milena
AU  - Matić, Dragana
AU  - Mrdaković, Marija
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1134
UR  - https://www.sciencedirect.com/science/article/abs/pii/S0196978112002641
AB  - Ghrelin is a 28-amino acid peptide that has significant effects on appetite and growth in humans and animals. The aim of this study was to examine 4th instar larvae of the pest insect Lymantria dispar L. after ghrelin treatment. Parameters included changes in nutritional indices (efficiency of conversion of ingested food, efficiency of conversion of digested food, approximate digestibility); midgut and fat body mass; total proteases, trypsin and leucine aminopeptidase activities in the midgut; number, height and width of columnar and goblet cells and their nuclei in the midgut epithelium and detection of ghrelin-like immunoreactivity in the midgut tissue. Four subpicomolar injections of ghrelin (0.3 pmol) or physiological saline (control) were applied every 24h. The nutritional indices were higher in the ghrelin treated than in the control group. Ghrelin treatment was also associated with elevation of midgut mass, induced digestive enzyme activities, increased fat body mass and morphometric changes in columnar and goblet cells. This is the first report of the presence of ghrelin-like hormone in endocrine cells of an insect midgut. Such information provides additional evidence for application of this relatively simple model system in the future studies of the mechanisms underlying of digestion and energy balance in more complex organisms.
PB  - Elsevier Science Inc
T2  - Peptides
T1  - Ghrelin effect on nutritional indices, midgut and fat body of Lymantria dispar L. (Lymantriidae)
IS  - 1
VL  - 37
DO  - 10.1016/j.peptides.2012.04.025
SP  - 55
EP  - 62
ER  - 

@article{
author = "Perić Mataruga, Vesna and Vlahović, Milena and Petković, Branka and Ilijin, Larisa and Janković Tomanić, Milena and Matić, Dragana and Mrdaković, Marija",
year = "2012",
abstract = "Ghrelin is a 28-amino acid peptide that has significant effects on appetite and growth in humans and animals. The aim of this study was to examine 4th instar larvae of the pest insect Lymantria dispar L. after ghrelin treatment. Parameters included changes in nutritional indices (efficiency of conversion of ingested food, efficiency of conversion of digested food, approximate digestibility); midgut and fat body mass; total proteases, trypsin and leucine aminopeptidase activities in the midgut; number, height and width of columnar and goblet cells and their nuclei in the midgut epithelium and detection of ghrelin-like immunoreactivity in the midgut tissue. Four subpicomolar injections of ghrelin (0.3 pmol) or physiological saline (control) were applied every 24h. The nutritional indices were higher in the ghrelin treated than in the control group. Ghrelin treatment was also associated with elevation of midgut mass, induced digestive enzyme activities, increased fat body mass and morphometric changes in columnar and goblet cells. This is the first report of the presence of ghrelin-like hormone in endocrine cells of an insect midgut. Such information provides additional evidence for application of this relatively simple model system in the future studies of the mechanisms underlying of digestion and energy balance in more complex organisms.",
publisher = "Elsevier Science Inc",
journal = "Peptides",
title = "Ghrelin effect on nutritional indices, midgut and fat body of Lymantria dispar L. (Lymantriidae)",
number = "1",
volume = "37",
doi = "10.1016/j.peptides.2012.04.025",
pages = "55-62"
}

Perić Mataruga, V., Vlahović, M., Petković, B., Ilijin, L., Janković Tomanić, M., Matić, D.,& Mrdaković, M.. (2012). Ghrelin effect on nutritional indices, midgut and fat body of Lymantria dispar L. (Lymantriidae). in Peptides
Elsevier Science Inc., 37(1), 55-62.
https://doi.org/10.1016/j.peptides.2012.04.025

Perić Mataruga V, Vlahović M, Petković B, Ilijin L, Janković Tomanić M, Matić D, Mrdaković M. Ghrelin effect on nutritional indices, midgut and fat body of Lymantria dispar L. (Lymantriidae). in Peptides. 2012;37(1):55-62.
doi:10.1016/j.peptides.2012.04.025 .

Perić Mataruga, Vesna, Vlahović, Milena, Petković, Branka, Ilijin, Larisa, Janković Tomanić, Milena, Matić, Dragana, Mrdaković, Marija, "Ghrelin effect on nutritional indices, midgut and fat body of Lymantria dispar L. (Lymantriidae)" in Peptides, 37, no. 1 (2012):55-62,
https://doi.org/10.1016/j.peptides.2012.04.025 . .

TY  - JOUR
AU  - Perić Mataruga, Vesna
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Ilijin, Larisa
AU  - Janković Tomanić, Milena
AU  - Mirčić, D.
AU  - Nenadović, Vera
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/280
AB  - The number, morphometric parameters and amount of aminergic neurosecretory product of protocerebral A2 neurosecretory neurons were investigated in the fifth instar of Lymantria dispar caterpillars, following a suitable or unsuitable trophic regime. Caterpillars originated from two populations (Quercus rubra or Robinia pseudoacacia forest) and were differently adapted to trophic stress, i.e. feeding on locust tree leaves - unsuitable host plant. The number of neurosecretory neurons was higher in the caterpillars originated from Robinia population than in Quercus population, regardless of feeding. A2 neurosecretory neurons, nuclei and their nucleoli were larger in caterpillars fed with unsuitable leaves in both populations. There was more aminergic product in the A2 neurosecretory neurons of the caterpillars fed with unsuitable leaves independently of population origin.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Biogenic amines in protocerebral A2 neurosecretory neurons of Lymantria dispar L. (Lepidoptera:Lymantriidae): Response to trophic stress
IS  - 3
VL  - 63
DO  - 10.2298/ABS1103571P
SP  - 571
EP  - 577
ER  - 

@article{
author = "Perić Mataruga, Vesna and Mrdaković, Marija and Vlahović, Milena and Ilijin, Larisa and Janković Tomanić, Milena and Mirčić, D. and Nenadović, Vera",
year = "2011, 2011",
abstract = "The number, morphometric parameters and amount of aminergic neurosecretory product of protocerebral A2 neurosecretory neurons were investigated in the fifth instar of Lymantria dispar caterpillars, following a suitable or unsuitable trophic regime. Caterpillars originated from two populations (Quercus rubra or Robinia pseudoacacia forest) and were differently adapted to trophic stress, i.e. feeding on locust tree leaves - unsuitable host plant. The number of neurosecretory neurons was higher in the caterpillars originated from Robinia population than in Quercus population, regardless of feeding. A2 neurosecretory neurons, nuclei and their nucleoli were larger in caterpillars fed with unsuitable leaves in both populations. There was more aminergic product in the A2 neurosecretory neurons of the caterpillars fed with unsuitable leaves independently of population origin.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Biogenic amines in protocerebral A2 neurosecretory neurons of Lymantria dispar L. (Lepidoptera:Lymantriidae): Response to trophic stress",
number = "3",
volume = "63",
doi = "10.2298/ABS1103571P",
pages = "571-577"
}

Perić Mataruga, V., Mrdaković, M., Vlahović, M., Ilijin, L., Janković Tomanić, M., Mirčić, D.,& Nenadović, V.. (2011). Biogenic amines in protocerebral A2 neurosecretory neurons of Lymantria dispar L. (Lepidoptera:Lymantriidae): Response to trophic stress. in Archives of Biological Sciences
Serbian Biological Society., 63(3), 571-577.
https://doi.org/10.2298/ABS1103571P

Perić Mataruga V, Mrdaković M, Vlahović M, Ilijin L, Janković Tomanić M, Mirčić D, Nenadović V. Biogenic amines in protocerebral A2 neurosecretory neurons of Lymantria dispar L. (Lepidoptera:Lymantriidae): Response to trophic stress. in Archives of Biological Sciences. 2011;63(3):571-577.
doi:10.2298/ABS1103571P .

Perić Mataruga, Vesna, Mrdaković, Marija, Vlahović, Milena, Ilijin, Larisa, Janković Tomanić, Milena, Mirčić, D., Nenadović, Vera, "Biogenic amines in protocerebral A2 neurosecretory neurons of Lymantria dispar L. (Lepidoptera:Lymantriidae): Response to trophic stress" in Archives of Biological Sciences, 63, no. 3 (2011):571-577,
https://doi.org/10.2298/ABS1103571P . .

TY  - JOUR
AU  - Mirčić, D.
AU  - Janković Tomanić, Milena
AU  - Nenadović, Vera
AU  - Franeta, F.
AU  - Lazarević, Jelica
PY  - 2010
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/264
AB  - Gypsy moth (Lymantria dispar L.) females and males were chronically exposed to three sublethal cadmium concentrations (10, 30 and 50 mg/g dry food mass) in order to assess the effects of cadmium on larval and pupal duration, pupal mass and longevity. On average, the presence of cadmium in food did not affect larval duration while shortened pupal duration and reduced pupal mass and longevity were recorded. The most significant effects were obtained at the highest cadmium concentration. Females and males did not differ in sensitivity of life history traits to cadmium exposure. It is concluded that (1) cadmium exerts a strong adverse impact on the growth and development of gypsy moths, and (2) the significance of the cadmium effects depends on the dose.
T2  - Archives of Biological Sciences
T1  - The effects of cadmium on the life history traits of Lymantria dispar L.
IS  - 4
VL  - 62
SP  - 1013
EP  - 1020
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_264
ER  - 

@article{
author = "Mirčić, D. and Janković Tomanić, Milena and Nenadović, Vera and Franeta, F. and Lazarević, Jelica",
year = "2010, 2010",
abstract = "Gypsy moth (Lymantria dispar L.) females and males were chronically exposed to three sublethal cadmium concentrations (10, 30 and 50 mg/g dry food mass) in order to assess the effects of cadmium on larval and pupal duration, pupal mass and longevity. On average, the presence of cadmium in food did not affect larval duration while shortened pupal duration and reduced pupal mass and longevity were recorded. The most significant effects were obtained at the highest cadmium concentration. Females and males did not differ in sensitivity of life history traits to cadmium exposure. It is concluded that (1) cadmium exerts a strong adverse impact on the growth and development of gypsy moths, and (2) the significance of the cadmium effects depends on the dose.",
journal = "Archives of Biological Sciences",
title = "The effects of cadmium on the life history traits of Lymantria dispar L.",
number = "4",
volume = "62",
pages = "1013-1020",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_264"
}

Mirčić, D., Janković Tomanić, M., Nenadović, V., Franeta, F.,& Lazarević, J.. (2010). The effects of cadmium on the life history traits of Lymantria dispar L.. in Archives of Biological Sciences, 62(4), 1013-1020.
https://hdl.handle.net/21.15107/rcub_ibiss_264

Mirčić D, Janković Tomanić M, Nenadović V, Franeta F, Lazarević J. The effects of cadmium on the life history traits of Lymantria dispar L.. in Archives of Biological Sciences. 2010;62(4):1013-1020.
https://hdl.handle.net/21.15107/rcub_ibiss_264 .

Mirčić, D., Janković Tomanić, Milena, Nenadović, Vera, Franeta, F., Lazarević, Jelica, "The effects of cadmium on the life history traits of Lymantria dispar L." in Archives of Biological Sciences, 62, no. 4 (2010):1013-1020,
https://hdl.handle.net/21.15107/rcub_ibiss_264 .

TY  - CONF
AU  - Ilijin, Larisa
AU  - Perić Mataruga, Vesna
AU  - Lazarević, Jelica
AU  - Prolić, Zlatko
AU  - Vlahović, Milena
AU  - Janković Tomanić, Milena
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3936
AB  - The neuroendocrine system of insects consists of neurosecretory neurons located in the brain and ventral nerve cord. Neurohormones are synthesized and secrete in neurosecretory neurons. Neurohormones represent the main regulators of physiological and metabolic processes including secretion of hormones that regulate molting, metamorphosis, reproduction, stress responses and homeostasis in insects. The dorsolateral neurosecretory neurons of insect protocerebrum are known to be a site of synthesis of allatostatins and large form of prothoracicotropic hormone, the regulators of metabolic and morphogenetic processes in insects. 
The effects of constant magnetic (average magnet induction of 235 mT) and extreme low frequency (average magnet induction of 6 mT) magnetic field on morphometric characteristics of L2' neurosecretory neurons in 4th instar gypsy moth larvae were investigated. Larvae were reared from first until third day of 4th instar on artificial diet and in constant and extreme low frequency magnetic field. 
The acute effect of constant magnetic field on morphometric characteristics of L2' neurosecretory neurons in gypsy moth larvae were not detected. The extreme low frequency magnetic field led to a decrease in L2' cells size. Both type of magnetic fields induced an increase in amount of neurosecretory material. After the exposure to constant magnetic field the neurosecretory material was medium size grained, while after the exposure to extreme low frequency magnetic filed the large size grained neurosecretory material was dominantly presented. In this paper the reduction in L2' neurosecretory activity was obtained. It is known that these cells synthesize neurohormones involved in morphogenesis, and their level is decreased in the middle of larval instar and also when the instar is prolonged which be one of stress response action.
PB  - Sežana: Higher Education Centre, Laboratory for Geomagnetism and Aeronomy
C3  - MGB - 2008 : abstract booklet. International Conference on Magnetism, Geomagnetism and Biomagnetism; 2008 Nov 7-8; Sežana, Slovenia
T1  - Changes in lateral protocerebral neurosecretory neurons of Lymantria dispar L. larvae after exposure to constant and extreme low frequency magnetic field
SP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3936
ER  - 

@conference{
author = "Ilijin, Larisa and Perić Mataruga, Vesna and Lazarević, Jelica and Prolić, Zlatko and Vlahović, Milena and Janković Tomanić, Milena",
year = "2008",
abstract = "The neuroendocrine system of insects consists of neurosecretory neurons located in the brain and ventral nerve cord. Neurohormones are synthesized and secrete in neurosecretory neurons. Neurohormones represent the main regulators of physiological and metabolic processes including secretion of hormones that regulate molting, metamorphosis, reproduction, stress responses and homeostasis in insects. The dorsolateral neurosecretory neurons of insect protocerebrum are known to be a site of synthesis of allatostatins and large form of prothoracicotropic hormone, the regulators of metabolic and morphogenetic processes in insects. 
The effects of constant magnetic (average magnet induction of 235 mT) and extreme low frequency (average magnet induction of 6 mT) magnetic field on morphometric characteristics of L2' neurosecretory neurons in 4th instar gypsy moth larvae were investigated. Larvae were reared from first until third day of 4th instar on artificial diet and in constant and extreme low frequency magnetic field. 
The acute effect of constant magnetic field on morphometric characteristics of L2' neurosecretory neurons in gypsy moth larvae were not detected. The extreme low frequency magnetic field led to a decrease in L2' cells size. Both type of magnetic fields induced an increase in amount of neurosecretory material. After the exposure to constant magnetic field the neurosecretory material was medium size grained, while after the exposure to extreme low frequency magnetic filed the large size grained neurosecretory material was dominantly presented. In this paper the reduction in L2' neurosecretory activity was obtained. It is known that these cells synthesize neurohormones involved in morphogenesis, and their level is decreased in the middle of larval instar and also when the instar is prolonged which be one of stress response action.",
publisher = "Sežana: Higher Education Centre, Laboratory for Geomagnetism and Aeronomy",
journal = "MGB - 2008 : abstract booklet. International Conference on Magnetism, Geomagnetism and Biomagnetism; 2008 Nov 7-8; Sežana, Slovenia",
title = "Changes in lateral protocerebral neurosecretory neurons of Lymantria dispar L. larvae after exposure to constant and extreme low frequency magnetic field",
pages = "16",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3936"
}

Ilijin, L., Perić Mataruga, V., Lazarević, J., Prolić, Z., Vlahović, M.,& Janković Tomanić, M.. (2008). Changes in lateral protocerebral neurosecretory neurons of Lymantria dispar L. larvae after exposure to constant and extreme low frequency magnetic field. in MGB - 2008 : abstract booklet. International Conference on Magnetism, Geomagnetism and Biomagnetism; 2008 Nov 7-8; Sežana, Slovenia
Sežana: Higher Education Centre, Laboratory for Geomagnetism and Aeronomy., 16.
https://hdl.handle.net/21.15107/rcub_ibiss_3936

Ilijin L, Perić Mataruga V, Lazarević J, Prolić Z, Vlahović M, Janković Tomanić M. Changes in lateral protocerebral neurosecretory neurons of Lymantria dispar L. larvae after exposure to constant and extreme low frequency magnetic field. in MGB - 2008 : abstract booklet. International Conference on Magnetism, Geomagnetism and Biomagnetism; 2008 Nov 7-8; Sežana, Slovenia. 2008;:16.
https://hdl.handle.net/21.15107/rcub_ibiss_3936 .

Ilijin, Larisa, Perić Mataruga, Vesna, Lazarević, Jelica, Prolić, Zlatko, Vlahović, Milena, Janković Tomanić, Milena, "Changes in lateral protocerebral neurosecretory neurons of Lymantria dispar L. larvae after exposure to constant and extreme low frequency magnetic field" in MGB - 2008 : abstract booklet. International Conference on Magnetism, Geomagnetism and Biomagnetism; 2008 Nov 7-8; Sežana, Slovenia (2008):16,
https://hdl.handle.net/21.15107/rcub_ibiss_3936 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Nenadović, Vera
AU  - Janković Tomanić, Milena
AU  - Milanović, S.
PY  - 2008
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/212
AB  - Periodic fluctuations in density impose different selection pressures on populations of outbreaking Lepidoptera due to changes in their nutritional environment. The maternal effects hypothesis of insect outbreak predicts the transmission of this nutritional "information" to subsequent generations and alterations in offspring life-history traits. To test for these time-delayed effects of the parental generation, we compared life-history traits and their variation and covariation among laboratory-reared gypsy moths hatched from egg masses collected from low- and medium-density populations. Decreased individual performance was recorded in offspring from the medium-density population, indicating reduced egg provisioning under crowding conditions. Genetic variance and covariance were also shown to be sensitive to density of the parental generation. In gypsy moths from the medium-density population, quantitative genetic analysis revealed significantly higher broad-sense heritabilities for development duration traits and demonstrated a trade-off between development duration and body size.
AB  - Lepidoptere sa eruptivnom populacionom dinamikom su izložene različitim selektivnim pritiscima tokom periodičnih fluktuacija brojnosti usled promena u nutritivnom kvalitetu njihove životne sredine. Hipoteza o ulozi materinskog efekta u pojavi prenamnoženja insekata predviđa prenos ove nutritivne "informacije" na sledeće generacije i promenu osobina životnog ciklusa kod potomaka. Da bismo testirali ovaj vremenski odložen uticaj parentalne generacije uporedili smo osobine životnog ciklusa, njihovu varijabilnost i međusobne korelacije između gubara gajenih u laboratoriji poreklom iz populacija niske i srednje brojnosti. Potomstvo poreklom iz populacije srednje brojnosti pokazalo je smanjenje individualne performanse što ukazuje na redukciju kvaliteta jaja sa porastom gustine populacije. Genetička varijansa i kovarijansa su takođe bile osetljive na gustinu roditeljske generacije. Kvantitativno-genetičkom analizom je dobijena veća heritabilnost u širem smislu za osobine trajanja razvića kao i uzajamno ograničenje između trajanja razvića i mase lutki gubara koje su poreklom iz populacije srednje brojnosti.
T2  - Archives of Biological Sciences
T1  - Genetička varijabilnost i korelacije između osobina životnog ciklusa gubara (Lymantria dispar L.) poreklom iz dve populacije iz Srbije
T1  - Genetic variation and correlations of life-history traits in gypsy moths (Lymantria dispar L.) from two populations in Serbia
IS  - 4
VL  - 60
SP  - 619
EP  - 627
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_212
ER  - 

@article{
author = "Lazarević, Jelica and Nenadović, Vera and Janković Tomanić, Milena and Milanović, S.",
year = "2008, 2008",
abstract = "Periodic fluctuations in density impose different selection pressures on populations of outbreaking Lepidoptera due to changes in their nutritional environment. The maternal effects hypothesis of insect outbreak predicts the transmission of this nutritional "information" to subsequent generations and alterations in offspring life-history traits. To test for these time-delayed effects of the parental generation, we compared life-history traits and their variation and covariation among laboratory-reared gypsy moths hatched from egg masses collected from low- and medium-density populations. Decreased individual performance was recorded in offspring from the medium-density population, indicating reduced egg provisioning under crowding conditions. Genetic variance and covariance were also shown to be sensitive to density of the parental generation. In gypsy moths from the medium-density population, quantitative genetic analysis revealed significantly higher broad-sense heritabilities for development duration traits and demonstrated a trade-off between development duration and body size., Lepidoptere sa eruptivnom populacionom dinamikom su izložene različitim selektivnim pritiscima tokom periodičnih fluktuacija brojnosti usled promena u nutritivnom kvalitetu njihove životne sredine. Hipoteza o ulozi materinskog efekta u pojavi prenamnoženja insekata predviđa prenos ove nutritivne "informacije" na sledeće generacije i promenu osobina životnog ciklusa kod potomaka. Da bismo testirali ovaj vremenski odložen uticaj parentalne generacije uporedili smo osobine životnog ciklusa, njihovu varijabilnost i međusobne korelacije između gubara gajenih u laboratoriji poreklom iz populacija niske i srednje brojnosti. Potomstvo poreklom iz populacije srednje brojnosti pokazalo je smanjenje individualne performanse što ukazuje na redukciju kvaliteta jaja sa porastom gustine populacije. Genetička varijansa i kovarijansa su takođe bile osetljive na gustinu roditeljske generacije. Kvantitativno-genetičkom analizom je dobijena veća heritabilnost u širem smislu za osobine trajanja razvića kao i uzajamno ograničenje između trajanja razvića i mase lutki gubara koje su poreklom iz populacije srednje brojnosti.",
journal = "Archives of Biological Sciences",
title = "Genetička varijabilnost i korelacije između osobina životnog ciklusa gubara (Lymantria dispar L.) poreklom iz dve populacije iz Srbije, Genetic variation and correlations of life-history traits in gypsy moths (Lymantria dispar L.) from two populations in Serbia",
number = "4",
volume = "60",
pages = "619-627",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_212"
}

Lazarević, J., Nenadović, V., Janković Tomanić, M.,& Milanović, S.. (2008). Genetička varijabilnost i korelacije između osobina životnog ciklusa gubara (Lymantria dispar L.) poreklom iz dve populacije iz Srbije. in Archives of Biological Sciences, 60(4), 619-627.
https://hdl.handle.net/21.15107/rcub_ibiss_212

Lazarević J, Nenadović V, Janković Tomanić M, Milanović S. Genetička varijabilnost i korelacije između osobina životnog ciklusa gubara (Lymantria dispar L.) poreklom iz dve populacije iz Srbije. in Archives of Biological Sciences. 2008;60(4):619-627.
https://hdl.handle.net/21.15107/rcub_ibiss_212 .

Lazarević, Jelica, Nenadović, Vera, Janković Tomanić, Milena, Milanović, S., "Genetička varijabilnost i korelacije između osobina životnog ciklusa gubara (Lymantria dispar L.) poreklom iz dve populacije iz Srbije" in Archives of Biological Sciences, 60, no. 4 (2008):619-627,
https://hdl.handle.net/21.15107/rcub_ibiss_212 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Perić Mataruga, Vesna
AU  - Nenadović, Vera
AU  - Janković Tomanić, Milena
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/175
T2  - Archives of Biological Sciences
T1  - Trypsin activity in the midgut of gypsy moth (Lymantria dispar L.)larvae during the intermolt period
IS  - 4
VL  - 59
SP  - 59
EP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_175
ER  - 

@article{
author = "Lazarević, Jelica and Perić Mataruga, Vesna and Nenadović, Vera and Janković Tomanić, Milena",
year = "2007, 2007",
journal = "Archives of Biological Sciences",
title = "Trypsin activity in the midgut of gypsy moth (Lymantria dispar L.)larvae during the intermolt period",
number = "4",
volume = "59",
pages = "59-60",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_175"
}

Lazarević, J., Perić Mataruga, V., Nenadović, V.,& Janković Tomanić, M.. (2007). Trypsin activity in the midgut of gypsy moth (Lymantria dispar L.)larvae during the intermolt period. in Archives of Biological Sciences, 59(4), 59-60.
https://hdl.handle.net/21.15107/rcub_ibiss_175

Lazarević J, Perić Mataruga V, Nenadović V, Janković Tomanić M. Trypsin activity in the midgut of gypsy moth (Lymantria dispar L.)larvae during the intermolt period. in Archives of Biological Sciences. 2007;59(4):59-60.
https://hdl.handle.net/21.15107/rcub_ibiss_175 .

Lazarević, Jelica, Perić Mataruga, Vesna, Nenadović, Vera, Janković Tomanić, Milena, "Trypsin activity in the midgut of gypsy moth (Lymantria dispar L.)larvae during the intermolt period" in Archives of Biological Sciences, 59, no. 4 (2007):59-60,
https://hdl.handle.net/21.15107/rcub_ibiss_175 .

TY  - JOUR
AU  - Mrdaković, Marija
AU  - Lazarević, Jelica
AU  - Perić Mataruga, Vesna
AU  - Janković Tomanić, Milena
AU  - Ilijin, Larisa
AU  - Vlahović, Milena
AU  - Mirčić, D.
AU  - Nenadović, Vera
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/169
T2  - Archives of Biological Sciences
T1  - Morphometric changes of corpora allata in Morimus funereus müls. (Cerambycidae) larvae during thermal stress
IS  - 3
VL  - 59
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_169
ER  - 

@article{
author = "Mrdaković, Marija and Lazarević, Jelica and Perić Mataruga, Vesna and Janković Tomanić, Milena and Ilijin, Larisa and Vlahović, Milena and Mirčić, D. and Nenadović, Vera",
year = "2007, 2007",
journal = "Archives of Biological Sciences",
title = "Morphometric changes of corpora allata in Morimus funereus müls. (Cerambycidae) larvae during thermal stress",
number = "3",
volume = "59",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_169"
}

Mrdaković, M., Lazarević, J., Perić Mataruga, V., Janković Tomanić, M., Ilijin, L., Vlahović, M., Mirčić, D.,& Nenadović, V.. (2007). Morphometric changes of corpora allata in Morimus funereus müls. (Cerambycidae) larvae during thermal stress. in Archives of Biological Sciences, 59(3), 47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_169

Mrdaković M, Lazarević J, Perić Mataruga V, Janković Tomanić M, Ilijin L, Vlahović M, Mirčić D, Nenadović V. Morphometric changes of corpora allata in Morimus funereus müls. (Cerambycidae) larvae during thermal stress. in Archives of Biological Sciences. 2007;59(3):47-48.
https://hdl.handle.net/21.15107/rcub_ibiss_169 .

Mrdaković, Marija, Lazarević, Jelica, Perić Mataruga, Vesna, Janković Tomanić, Milena, Ilijin, Larisa, Vlahović, Milena, Mirčić, D., Nenadović, Vera, "Morphometric changes of corpora allata in Morimus funereus müls. (Cerambycidae) larvae during thermal stress" in Archives of Biological Sciences, 59, no. 3 (2007):47-48,
https://hdl.handle.net/21.15107/rcub_ibiss_169 .

TY  - JOUR
AU  - Mrdaković, Marija
AU  - Ilijin, Larisa
AU  - Janković Tomanić, Milena
AU  - Vlahović, Milena
AU  - Prolić, Zlatko M.
AU  - Perić Mataruga, Vesna
AU  - Lazarević, Jelica
AU  - Nenadović, Vera
PY  - 2005
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/112
AB  - The effects of different temperatures (23°C and 8°C) on activity of corpora allata (CA) and dorsolateral (L1, L2) protocerebral neurosecretory neurons were investigated in Morimus funereus Mulsant (1863) larvae collected from a natural population during March. Activity of CA was revealed by monitoring of CA volume and cell number. Increase of CA volume after two day exposure to both temperatures was shown to be the result of increase in cell number. Activity of CA was higher at 23°C than 8°C. Activity of L1 and L2 neurosecretory neurons was inhibited at both temperatures. Neurosecretory neurons were more sensitive to temperature of 23°C than 8°C. It can be supposed that dorsolateral neurosecretory neurons synthesize neurohormones that affect CA activity, depending on environmental temperature.
AB  - Ispitivan je uticaj različitih temperatura (23°C i 8°C) na aktivnost corpora allata (CA) i dorzolateralnih (L1 i L2) protocerebralnih neurosekretnih neurona kod larvi Morimus funereus Muls. (1863), sakupljenih iz prirode tokom meseca marta. Aktivnost CA je procenjivana praćenjem promena veličine CA i broja ćelija CA. Pokazano je da je povećanje veličine CA, nakon 2 dana izlaganja larvi obema temperaturama, rezultuje u povećanju broja ćelija. Aktivnost CA je veća na temperaturi od 230C, nego na 80C. Aktivnost L1 i L2 neurosekretnih neurona je bila inhibirana delovanjem obeju temperatura. Neurosekretni neuroni su osetljiviji na delovanje temperature od 230C, nego na 80C. Moglo bi se pretpostaviti da dorzolateralni neurosekretni neuroni sintetišu neurohormone koji utiču na aktivnost CA, u zavisnosti od temperature spoljašnje sredine.
T2  - Archives of Biological Sciences
T1  - Uticaj različitih temperatura na aktivnost corpora allata i dorzolateralnih protocerebralnih neurosekretnih neurona kod larvi Morimus funereus
T1  - Effects of thermal stress on activity of corpora allata and dorsolateral neurosecretory neurons in Morimus funereus larvae
IS  - 2
VL  - 57
SP  - 83
EP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_112
ER  - 

@article{
author = "Mrdaković, Marija and Ilijin, Larisa and Janković Tomanić, Milena and Vlahović, Milena and Prolić, Zlatko M. and Perić Mataruga, Vesna and Lazarević, Jelica and Nenadović, Vera",
year = "2005, 2005",
abstract = "The effects of different temperatures (23°C and 8°C) on activity of corpora allata (CA) and dorsolateral (L1, L2) protocerebral neurosecretory neurons were investigated in Morimus funereus Mulsant (1863) larvae collected from a natural population during March. Activity of CA was revealed by monitoring of CA volume and cell number. Increase of CA volume after two day exposure to both temperatures was shown to be the result of increase in cell number. Activity of CA was higher at 23°C than 8°C. Activity of L1 and L2 neurosecretory neurons was inhibited at both temperatures. Neurosecretory neurons were more sensitive to temperature of 23°C than 8°C. It can be supposed that dorsolateral neurosecretory neurons synthesize neurohormones that affect CA activity, depending on environmental temperature., Ispitivan je uticaj različitih temperatura (23°C i 8°C) na aktivnost corpora allata (CA) i dorzolateralnih (L1 i L2) protocerebralnih neurosekretnih neurona kod larvi Morimus funereus Muls. (1863), sakupljenih iz prirode tokom meseca marta. Aktivnost CA je procenjivana praćenjem promena veličine CA i broja ćelija CA. Pokazano je da je povećanje veličine CA, nakon 2 dana izlaganja larvi obema temperaturama, rezultuje u povećanju broja ćelija. Aktivnost CA je veća na temperaturi od 230C, nego na 80C. Aktivnost L1 i L2 neurosekretnih neurona je bila inhibirana delovanjem obeju temperatura. Neurosekretni neuroni su osetljiviji na delovanje temperature od 230C, nego na 80C. Moglo bi se pretpostaviti da dorzolateralni neurosekretni neuroni sintetišu neurohormone koji utiču na aktivnost CA, u zavisnosti od temperature spoljašnje sredine.",
journal = "Archives of Biological Sciences",
title = "Uticaj različitih temperatura na aktivnost corpora allata i dorzolateralnih protocerebralnih neurosekretnih neurona kod larvi Morimus funereus, Effects of thermal stress on activity of corpora allata and dorsolateral neurosecretory neurons in Morimus funereus larvae",
number = "2",
volume = "57",
pages = "83-92",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_112"
}

Mrdaković, M., Ilijin, L., Janković Tomanić, M., Vlahović, M., Prolić, Z. M., Perić Mataruga, V., Lazarević, J.,& Nenadović, V.. (2005). Uticaj različitih temperatura na aktivnost corpora allata i dorzolateralnih protocerebralnih neurosekretnih neurona kod larvi Morimus funereus. in Archives of Biological Sciences, 57(2), 83-92.
https://hdl.handle.net/21.15107/rcub_ibiss_112

Mrdaković M, Ilijin L, Janković Tomanić M, Vlahović M, Prolić ZM, Perić Mataruga V, Lazarević J, Nenadović V. Uticaj različitih temperatura na aktivnost corpora allata i dorzolateralnih protocerebralnih neurosekretnih neurona kod larvi Morimus funereus. in Archives of Biological Sciences. 2005;57(2):83-92.
https://hdl.handle.net/21.15107/rcub_ibiss_112 .

Mrdaković, Marija, Ilijin, Larisa, Janković Tomanić, Milena, Vlahović, Milena, Prolić, Zlatko M., Perić Mataruga, Vesna, Lazarević, Jelica, Nenadović, Vera, "Uticaj različitih temperatura na aktivnost corpora allata i dorzolateralnih protocerebralnih neurosekretnih neurona kod larvi Morimus funereus" in Archives of Biological Sciences, 57, no. 2 (2005):83-92,
https://hdl.handle.net/21.15107/rcub_ibiss_112 .

TY  - CONF
AU  - Vlahović, Milena
AU  - Lazarević, Jelica
AU  - Ilijin, Larisa
AU  - Perić Mataruga, Vesna
AU  - Mrdaković, Marija
AU  - Janković Tomanić, Milena
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3976
AB  - Chronic effects of two cadmium concentrations (10 and 30 µg Cd/g dry food) on larval mass and midgut phosphatase activity (total, acid lysosomal and alkaline), were investigated in the 4th instar of the gypsy moth larvae (Lymantria dispar L.). Native PAGE was used for detection of phosphatases isozymes using p-nitrophenyl phosphate as a substrate. The analysis was performed on 20 egg masses (5 larvae/ egg mass/ treatment). It was found that both cadmium concentrations had inhibitory effect on phosphatase activity (total, lysosomal and alkaline). Only higher concentration (30 µg Cd/g dry food) reduced larval mass. Electrophoretograms of acid and alkaline phosphatases showed three major isoforms which differed in the level of expression depending on egg mass and cadmium treatment. Variability of alkaline phosphatase at 10 µg Cd/g dry food was lower than at the control treatment, while variance of lysosomal phosphatase was significantly higher at 30 µg Cd/g compared to control. As midgut homogenates were pulled within each egg mass (full-sib family), the change in a trait variance represented the change in genetic diversity. Significant and positive correlations for alkaline phosphatase between control and two cadmium concentrations as well as between the two cadmium treatments showed common genetic determination of a trait in the suitable and stressful environment. Common genetic determination was also observed between two cadmium concentrations for larval mass. The absence of significant correlations for lysosomal phosphatase between different environments might point to expression of different genes. Decrease of lysosomal phosphatase activity at both cadmium concentrations, increase of variance at 30 µg Cd/g and lack of significant correlations between different treatments indicate that this enzyme could be indicator of environmental changes.
PB  - Belgrade: Plant Protection Society of Serbia
C3  - 5th Congress of Plant Protection; 2004 Nov 22-26; Zlatibor, Srbija; Book of Abstract pp. 72-73
T1  - Chronic effect of cadmium on phosphatase activity in the midgut of gypsy moth larvae
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3976
ER  - 

@conference{
author = "Vlahović, Milena and Lazarević, Jelica and Ilijin, Larisa and Perić Mataruga, Vesna and Mrdaković, Marija and Janković Tomanić, Milena",
year = "2004",
abstract = "Chronic effects of two cadmium concentrations (10 and 30 µg Cd/g dry food) on larval mass and midgut phosphatase activity (total, acid lysosomal and alkaline), were investigated in the 4th instar of the gypsy moth larvae (Lymantria dispar L.). Native PAGE was used for detection of phosphatases isozymes using p-nitrophenyl phosphate as a substrate. The analysis was performed on 20 egg masses (5 larvae/ egg mass/ treatment). It was found that both cadmium concentrations had inhibitory effect on phosphatase activity (total, lysosomal and alkaline). Only higher concentration (30 µg Cd/g dry food) reduced larval mass. Electrophoretograms of acid and alkaline phosphatases showed three major isoforms which differed in the level of expression depending on egg mass and cadmium treatment. Variability of alkaline phosphatase at 10 µg Cd/g dry food was lower than at the control treatment, while variance of lysosomal phosphatase was significantly higher at 30 µg Cd/g compared to control. As midgut homogenates were pulled within each egg mass (full-sib family), the change in a trait variance represented the change in genetic diversity. Significant and positive correlations for alkaline phosphatase between control and two cadmium concentrations as well as between the two cadmium treatments showed common genetic determination of a trait in the suitable and stressful environment. Common genetic determination was also observed between two cadmium concentrations for larval mass. The absence of significant correlations for lysosomal phosphatase between different environments might point to expression of different genes. Decrease of lysosomal phosphatase activity at both cadmium concentrations, increase of variance at 30 µg Cd/g and lack of significant correlations between different treatments indicate that this enzyme could be indicator of environmental changes.",
publisher = "Belgrade: Plant Protection Society of Serbia",
journal = "5th Congress of Plant Protection; 2004 Nov 22-26; Zlatibor, Srbija; Book of Abstract pp. 72-73",
title = "Chronic effect of cadmium on phosphatase activity in the midgut of gypsy moth larvae",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3976"
}

Vlahović, M., Lazarević, J., Ilijin, L., Perić Mataruga, V., Mrdaković, M.,& Janković Tomanić, M.. (2004). Chronic effect of cadmium on phosphatase activity in the midgut of gypsy moth larvae. in 5th Congress of Plant Protection; 2004 Nov 22-26; Zlatibor, Srbija; Book of Abstract pp. 72-73
Belgrade: Plant Protection Society of Serbia..
https://hdl.handle.net/21.15107/rcub_ibiss_3976

Vlahović M, Lazarević J, Ilijin L, Perić Mataruga V, Mrdaković M, Janković Tomanić M. Chronic effect of cadmium on phosphatase activity in the midgut of gypsy moth larvae. in 5th Congress of Plant Protection; 2004 Nov 22-26; Zlatibor, Srbija; Book of Abstract pp. 72-73. 2004;.
https://hdl.handle.net/21.15107/rcub_ibiss_3976 .

Vlahović, Milena, Lazarević, Jelica, Ilijin, Larisa, Perić Mataruga, Vesna, Mrdaković, Marija, Janković Tomanić, Milena, "Chronic effect of cadmium on phosphatase activity in the midgut of gypsy moth larvae" in 5th Congress of Plant Protection; 2004 Nov 22-26; Zlatibor, Srbija; Book of Abstract pp. 72-73 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_3976 .