TY  - JOUR
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Jankovský, Libor
AU  - Lazarević, Jelica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5299
AB  - Botanicals, such as essential oils (EO) and their compounds, are considered a viable ecofriendly
alternative to synthetic insecticides, which threaten human health and ecosystem functioning.
In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid)
and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious
pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology
were assessed at different compound concentrations and compared with the effects of the commercial
botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed
by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice
assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations
of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing
molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth
regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and
food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food
processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results
suggest that anethole and carvone might be used as control agents against GML.
PB  - Basel: MDPI
T2  - Agronomy
T1  - Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)
IS  - 12
VL  - 12
DO  - 10.3390/agronomy12123049
SP  - 3049
ER  - 

@article{
author = "Kostić, Igor and Milanović, Slobodan and Kostić, Miroslav and Šešlija Jovanović, Darka and Ćalić, Dušica and Jankovský, Libor and Lazarević, Jelica",
year = "2022",
abstract = "Botanicals, such as essential oils (EO) and their compounds, are considered a viable ecofriendly
alternative to synthetic insecticides, which threaten human health and ecosystem functioning.
In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid)
and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious
pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology
were assessed at different compound concentrations and compared with the effects of the commercial
botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed
by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice
assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations
of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing
molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth
regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and
food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food
processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results
suggest that anethole and carvone might be used as control agents against GML.",
publisher = "Basel: MDPI",
journal = "Agronomy",
title = "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)",
number = "12",
volume = "12",
doi = "10.3390/agronomy12123049",
pages = "3049"
}

Kostić, I., Milanović, S., Kostić, M., Šešlija Jovanović, D., Ćalić, D., Jankovský, L.,& Lazarević, J.. (2022). Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy
Basel: MDPI., 12(12), 3049.
https://doi.org/10.3390/agronomy12123049

Kostić I, Milanović S, Kostić M, Šešlija Jovanović D, Ćalić D, Jankovský L, Lazarević J. Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy. 2022;12(12):3049.
doi:10.3390/agronomy12123049 .

Kostić, Igor, Milanović, Slobodan, Kostić, Miroslav, Šešlija Jovanović, Darka, Ćalić, Dušica, Jankovský, Libor, Lazarević, Jelica, "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)" in Agronomy, 12, no. 12 (2022):3049,
https://doi.org/10.3390/agronomy12123049 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5306
AB  - The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae
species. The use of essential oil compounds in pest management has been proposed as an alternative
to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined
the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and
a thujone‐camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according
to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of
potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased
at higher concentrations of the examined compounds. Camphor was more effective against larvae
and the thujone‐camphor mixture was more effective against adults. Additionally, adults moved
faster towards the control leaf disc in the two‐choice olfactometer assay if an alternative disc was
treated with a thujone‐camphor mixture, whereas larvae responded similarly to the two potential
repellents. However, after contact with the leaf disc treated with the highest compound concentration,
the larvae escaped faster from the thujone‐camphor mixture than from pure camphor. In conclusion,
both examined compounds are promising eco‐friendly antifeedants, but their efficacy depends
on the developmental stage of the beetle, compound type and applied concentration.
PB  - Basel: MDPI
T2  - Plants
T1  - Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle
IS  - 24
VL  - 11
DO  - 10.3390/plants11243587
SP  - 3587
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Šešlija Jovanović, Darka and Ćalić, Dušica and Milanović, Slobodan and Kostić, Miroslav",
year = "2022",
abstract = "The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae
species. The use of essential oil compounds in pest management has been proposed as an alternative
to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined
the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and
a thujone‐camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according
to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of
potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased
at higher concentrations of the examined compounds. Camphor was more effective against larvae
and the thujone‐camphor mixture was more effective against adults. Additionally, adults moved
faster towards the control leaf disc in the two‐choice olfactometer assay if an alternative disc was
treated with a thujone‐camphor mixture, whereas larvae responded similarly to the two potential
repellents. However, after contact with the leaf disc treated with the highest compound concentration,
the larvae escaped faster from the thujone‐camphor mixture than from pure camphor. In conclusion,
both examined compounds are promising eco‐friendly antifeedants, but their efficacy depends
on the developmental stage of the beetle, compound type and applied concentration.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle",
number = "24",
volume = "11",
doi = "10.3390/plants11243587",
pages = "3587"
}

Lazarević, J., Kostić, I., Šešlija Jovanović, D., Ćalić, D., Milanović, S.,& Kostić, M.. (2022). Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants
Basel: MDPI., 11(24), 3587.
https://doi.org/10.3390/plants11243587

Lazarević J, Kostić I, Šešlija Jovanović D, Ćalić D, Milanović S, Kostić M. Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants. 2022;11(24):3587.
doi:10.3390/plants11243587 .

Lazarević, Jelica, Kostić, Igor, Šešlija Jovanović, Darka, Ćalić, Dušica, Milanović, Slobodan, Kostić, Miroslav, "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle" in Plants, 11, no. 24 (2022):3587,
https://doi.org/10.3390/plants11243587 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Kostić, Miroslav
AU  - Šešlija Jovanović, Darka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4799
AB  - Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Physiology
T1  - Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say
VL  - 13
DO  - 10.3389/fphys.2022.842314
SP  - 842314
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Vuleta, Ana and Manitašević Jovanović, Sanja and Kostić, Miroslav and Šešlija Jovanović, Darka",
year = "2022",
abstract = "Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Physiology",
title = "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say",
volume = "13",
doi = "10.3389/fphys.2022.842314",
pages = "842314"
}

Lazarević, J., Jevremović, S., Kostić, I., Vuleta, A., Manitašević Jovanović, S., Kostić, M.,& Šešlija Jovanović, D.. (2022). Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology
Lausanne: Frontiers Media SA., 13, 842314.
https://doi.org/10.3389/fphys.2022.842314

Lazarević J, Jevremović S, Kostić I, Vuleta A, Manitašević Jovanović S, Kostić M, Šešlija Jovanović D. Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology. 2022;13:842314.
doi:10.3389/fphys.2022.842314 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Vuleta, Ana, Manitašević Jovanović, Sanja, Kostić, Miroslav, Šešlija Jovanović, Darka, "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say" in Frontiers in Physiology, 13 (2022):842314,
https://doi.org/10.3389/fphys.2022.842314 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Šešlija Jovanović, Darka
AU  - Kostić, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4504
AB  - The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood
forests. In the search for an environmentally safe means of its control, we assessed the impact
of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise
Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality,
molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with
commercial insecticide NeemAzal®-T/S (neem). The main compounds in the Eos were trans-anethole
in anise; carvone, limonene, and  -phellandrene in dill; and trans-anethole and fenchone in fennel
seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more
toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%,
anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs
were more effective in reducing growth rate, approximate digestibility and efficiency of conversion
of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and
metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from
both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used
for gypsy moth control.
PB  - Basel: MDPI
T2  - Plants
T1  - Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control
IS  - 10
VL  - 10
DO  - 10.3390/plants10102194
SP  - 2194
ER  - 

@article{
author = "Kostić, Igor and Lazarević, Jelica and Šešlija Jovanović, Darka and Kostić, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood
forests. In the search for an environmentally safe means of its control, we assessed the impact
of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise
Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality,
molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with
commercial insecticide NeemAzal®-T/S (neem). The main compounds in the Eos were trans-anethole
in anise; carvone, limonene, and  -phellandrene in dill; and trans-anethole and fenchone in fennel
seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more
toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%,
anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs
were more effective in reducing growth rate, approximate digestibility and efficiency of conversion
of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and
metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from
both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used
for gypsy moth control.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control",
number = "10",
volume = "10",
doi = "10.3390/plants10102194",
pages = "2194"
}

Kostić, I., Lazarević, J., Šešlija Jovanović, D., Kostić, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants
Basel: MDPI., 10(10), 2194.
https://doi.org/10.3390/plants10102194

Kostić I, Lazarević J, Šešlija Jovanović D, Kostić M, Marković T, Milanović S. Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants. 2021;10(10):2194.
doi:10.3390/plants10102194 .

Kostić, Igor, Lazarević, Jelica, Šešlija Jovanović, Darka, Kostić, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control" in Plants, 10, no. 10 (2021):2194,
https://doi.org/10.3390/plants10102194 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Šešlija Jovanović, Darka
AU  - Krnjajić, Slobodan
AU  - Ćalić, Dušica
AU  - Stanković, Slađan
AU  - Kostić, Miroslav
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3838
AB  - The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.
PB  - Cambridge University Press
T2  - Bulletin of Entomological Research
T1  - Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)
IS  - 2
VL  - 11
DO  - 10.1017/S0007485320000504
SP  - 190
EP  - 199
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Milanović, Slobodan and Šešlija Jovanović, Darka and Krnjajić, Slobodan and Ćalić, Dušica and Stanković, Slađan and Kostić, Miroslav",
year = "2021",
abstract = "The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.",
publisher = "Cambridge University Press",
journal = "Bulletin of Entomological Research",
title = "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)",
number = "2",
volume = "11",
doi = "10.1017/S0007485320000504",
pages = "190-199"
}

Lazarević, J., Kostić, I., Milanović, S., Šešlija Jovanović, D., Krnjajić, S., Ćalić, D., Stanković, S.,& Kostić, M.. (2021). Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research
Cambridge University Press., 11(2), 190-199.
https://doi.org/10.1017/S0007485320000504

Lazarević J, Kostić I, Milanović S, Šešlija Jovanović D, Krnjajić S, Ćalić D, Stanković S, Kostić M. Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research. 2021;11(2):190-199.
doi:10.1017/S0007485320000504 .

Lazarević, Jelica, Kostić, Igor, Milanović, Slobodan, Šešlija Jovanović, Darka, Krnjajić, Slobodan, Ćalić, Dušica, Stanković, Slađan, Kostić, Miroslav, "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)" in Bulletin of Entomological Research, 11, no. 2 (2021):190-199,
https://doi.org/10.1017/S0007485320000504 . .

TY  - DATA
AU  - Kostić, igor
AU  - Lazarević, Jelica
AU  - Šešlija Jovanović, Darka
AU  - Kostić, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4465
AB  - We provide one excel (with four sheets) and 4 csv files. The data in the excel is the same as the one provided in the 4 csv files. 

Data file named 'data plants 1393603.xls' is consisted of 4 sheets containing raw data on all analyzed traits in the manuscript. 

The first sheet named 'antifeeding' contains data on absolute and relative deterrence coefficients of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The second sheet named 'mortality' contains data on the percentage of mortality of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The third sheet named 'molting' contains data on the percentage of 2nd to 3rd instar molting and percentage of molting reduction of gypsy moth larvae exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The forth sheet named 'growth and nutritional indices' contains data on the initial mass, amount of consumed, assimilated and metabolized food, and indices RGR, RCR, RMR, ECI, AD, ECD, and metabolic cost of gypsy moth larvae (4th instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard.
PB  - Basel: MDPI
T2  - Plants
T1  - Potential of Essential Oils for the Gypsy Moth Control
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4465
ER  - 

@misc{
author = "Kostić, igor and Lazarević, Jelica and Šešlija Jovanović, Darka and Kostić, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "We provide one excel (with four sheets) and 4 csv files. The data in the excel is the same as the one provided in the 4 csv files. 

Data file named 'data plants 1393603.xls' is consisted of 4 sheets containing raw data on all analyzed traits in the manuscript. 

The first sheet named 'antifeeding' contains data on absolute and relative deterrence coefficients of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The second sheet named 'mortality' contains data on the percentage of mortality of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The third sheet named 'molting' contains data on the percentage of 2nd to 3rd instar molting and percentage of molting reduction of gypsy moth larvae exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The forth sheet named 'growth and nutritional indices' contains data on the initial mass, amount of consumed, assimilated and metabolized food, and indices RGR, RCR, RMR, ECI, AD, ECD, and metabolic cost of gypsy moth larvae (4th instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Potential of Essential Oils for the Gypsy Moth Control",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4465"
}

Kostić, i., Lazarević, J., Šešlija Jovanović, D., Kostić, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils for the Gypsy Moth Control. in Plants
Basel: MDPI..
https://hdl.handle.net/21.15107/rcub_ibiss_4465

Kostić I, Lazarević J, Šešlija Jovanović D, Kostić M, Marković T, Milanović S. Potential of Essential Oils for the Gypsy Moth Control. in Plants. 2021;.
https://hdl.handle.net/21.15107/rcub_ibiss_4465 .

Kostić, igor, Lazarević, Jelica, Šešlija Jovanović, Darka, Kostić, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils for the Gypsy Moth Control" in Plants (2021),
https://hdl.handle.net/21.15107/rcub_ibiss_4465 .

TY  - JOUR
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Savić, Jelena
AU  - Ćalić, Dušica
PY  - 2020
UR  - internal-pdf://Devrnja et al. - 2020 - Evaluation of tansy essential oil as a potential “green” alternative for gypsy moth control.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3601
AB  - The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality (< 10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet (digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.
T2  - Environmental Science and Pollution Research
T1  - Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.
VL  - 27
DO  - 10.1007/s11356-020-07825-1
SP  - 11958
EP  - 11967
ER  - 

@article{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Savić, Jelena and Ćalić, Dušica",
year = "2020",
abstract = "The development of "green" alternatives to chemical pesticides could play a crucial role in integrated pest management (IPM). Their use is considered either as a substitution for or in addition to hazardous synthetic products. We analysed the influence of three concentrations of tansy (Tanacetum vulgare L.) essential oil (EO), previously characterised by GC-MS, on the survival and moulting of the 2nd instar and the nutritional indices of the 4th instar gypsy moth (Lymantria dispar L.) larvae. In a residual contact toxicity assessment, the exposure to tansy EO caused low mortality (< 10%) while larval development was significantly slowed down, i.e., the percentage of larvae that moulted into the 3rd instar was reduced. On the other hand, when tansy EO was incorporated into the diet (digestive toxicity assay), high mortality and a lack of moulting after 120 h of eating were recorded for the highest applied concentration of EO. During 48 h of feeding on EO-supplemented food at concentrations of 0.5 and 1% (v/v), the relative growth rate (RGR) of the 4th instar larvae significantly decreased, which can be explained by a significant reduction of the relative consumption rate (RCR) and significantly or marginally significantly lower efficiency of conversion of ingested food into insect biomass (ECI). Although the RCR was also reduced with the lowest applied EO concentration (0.1%), the ECI was not affected which meant the RGR was as high as it was for the control larvae. ECI changes, when two higher EO concentrations were applied, were due to a reduction in the efficiency of conversion of digested food into biomass (ECD), while approximate digestibility was unaffected by the presence of EO in the food. Our results on the significant negative effects of tansy EO on gypsy moth larval survival, development time, and nutritional physiology suggest that it could be considered in future designs for botanical insecticides for gypsy moth control.",
journal = "Environmental Science and Pollution Research",
title = "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.",
volume = "27",
doi = "10.1007/s11356-020-07825-1",
pages = "11958-11967"
}

Devrnja, N., Kostić, I., Lazarević, J., Savić, J.,& Ćalić, D.. (2020). Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.. in Environmental Science and Pollution Research, 27, 11958-11967.
https://doi.org/10.1007/s11356-020-07825-1

Devrnja N, Kostić I, Lazarević J, Savić J, Ćalić D. Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control.. in Environmental Science and Pollution Research. 2020;27:11958-11967.
doi:10.1007/s11356-020-07825-1 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Savić, Jelena, Ćalić, Dušica, "Evaluation of tansy essential oil as a potential "green" alternative for gypsy moth control." in Environmental Science and Pollution Research, 27 (2020):11958-11967,
https://doi.org/10.1007/s11356-020-07825-1 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Kostić, Miroslav
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Šešlija Jovanović, Darka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3837
AB  - The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Insects
T1  - Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus
IS  - 9
VL  - 11
DO  - 10.3390/insects11090563
SP  - 563
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Kostić, Miroslav and Vuleta, Ana and Manitašević Jovanović, Sanja and Šešlija Jovanović, Darka",
year = "2020",
abstract = "The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Insects",
title = "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus",
number = "9",
volume = "11",
doi = "10.3390/insects11090563",
pages = "563"
}

Lazarević, J., Jevremović, S., Kostić, I., Kostić, M., Vuleta, A., Manitašević Jovanović, S.,& Šešlija Jovanović, D.. (2020). Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects
Multidisciplinary Digital Publishing Institute., 11(9), 563.
https://doi.org/10.3390/insects11090563

Lazarević J, Jevremović S, Kostić I, Kostić M, Vuleta A, Manitašević Jovanović S, Šešlija Jovanović D. Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects. 2020;11(9):563.
doi:10.3390/insects11090563 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Kostić, Miroslav, Vuleta, Ana, Manitašević Jovanović, Sanja, Šešlija Jovanović, Darka, "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus" in Insects, 11, no. 9 (2020):563,
https://doi.org/10.3390/insects11090563 . .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Popović, Marija
AU  - Dobrosavljević, Jovan
AU  - Kostić, Igor
AU  - Lazarević, Jelica
PY  - 2020
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4780
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3653
AB  - Gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) feeds on a large number of tree species, while ash, Fraxinus spp. (Lamiales: Oleaceae) species are considered resistant and are only sporadically eaten. To assess the conditions under which late instar gypsy moth larvae (GML) can temporarily use non-host common ash (CA) (F. excelsior L.), and to evaluate their ability to recover from ingestion of this toxic food, we determined the relative growth rate, the relative consumption rate and the amount of produced feces in different laboratory feeding trials. Our report is the first to show that under specific circumstances, the resources acquired after short-term consumption of CA leaves can be utilized for larval growth. We varied the intensity of density and starvation stress prior to feeding on CA leaves. We observed that after moderate stress a group of GML was temporarily capable of coping with CA leaves. Although observed growth and consumption were much lower on CA than on the optimal host oak, Quercus cerris L. (Fagales: Fagaceae), CA-oak-switched larvae showed the ability to recover from short-term use of a toxic non-host foliage. This suggests that feeding on CA might enable GML to survive under conditions of food shortage.
T2  - Archives of Biological Sciences
T1  - Desperate times call for desperate measures: Short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress
IS  - 1
VL  - 72
DO  - 10.2298/abs191106067m
SP  - 63
EP  - 69
ER  - 

@article{
author = "Milanović, Slobodan and Popović, Marija and Dobrosavljević, Jovan and Kostić, Igor and Lazarević, Jelica",
year = "2020",
abstract = "Gypsy moth, Lymantria dispar L. (Lepidoptera: Erebidae) feeds on a large number of tree species, while ash, Fraxinus spp. (Lamiales: Oleaceae) species are considered resistant and are only sporadically eaten. To assess the conditions under which late instar gypsy moth larvae (GML) can temporarily use non-host common ash (CA) (F. excelsior L.), and to evaluate their ability to recover from ingestion of this toxic food, we determined the relative growth rate, the relative consumption rate and the amount of produced feces in different laboratory feeding trials. Our report is the first to show that under specific circumstances, the resources acquired after short-term consumption of CA leaves can be utilized for larval growth. We varied the intensity of density and starvation stress prior to feeding on CA leaves. We observed that after moderate stress a group of GML was temporarily capable of coping with CA leaves. Although observed growth and consumption were much lower on CA than on the optimal host oak, Quercus cerris L. (Fagales: Fagaceae), CA-oak-switched larvae showed the ability to recover from short-term use of a toxic non-host foliage. This suggests that feeding on CA might enable GML to survive under conditions of food shortage.",
journal = "Archives of Biological Sciences",
title = "Desperate times call for desperate measures: Short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress",
number = "1",
volume = "72",
doi = "10.2298/abs191106067m",
pages = "63-69"
}

Milanović, S., Popović, M., Dobrosavljević, J., Kostić, I.,& Lazarević, J.. (2020). Desperate times call for desperate measures: Short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress. in Archives of Biological Sciences, 72(1), 63-69.
https://doi.org/10.2298/abs191106067m

Milanović S, Popović M, Dobrosavljević J, Kostić I, Lazarević J. Desperate times call for desperate measures: Short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress. in Archives of Biological Sciences. 2020;72(1):63-69.
doi:10.2298/abs191106067m .

Milanović, Slobodan, Popović, Marija, Dobrosavljević, Jovan, Kostić, Igor, Lazarević, Jelica, "Desperate times call for desperate measures: Short-term use of the common ash tree by gypsy moth larvae (Lepidoptera: Erebidae) under density and starvation stress" in Archives of Biological Sciences, 72, no. 1 (2020):63-69,
https://doi.org/10.2298/abs191106067m . .

TY  - JOUR
AU  - Jevremović, Stojan
AU  - Lazarević, Jelica
AU  - Kostić, Miroslav
AU  - Krnjajić, Slobodan
AU  - Ugrenović, Vladan
AU  - Radonjić, Anda
AU  - Kostić, Igor
PY  - 2019
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4391
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3657
AB  - The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, α-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and α-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.
T2  - Archives of Biological Sciences
T1  - Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans
IS  - 4
VL  - 71
DO  - 10.2298/ABS190617049J
SP  - 665
EP  - 676
ER  - 

@article{
author = "Jevremović, Stojan and Lazarević, Jelica and Kostić, Miroslav and Krnjajić, Slobodan and Ugrenović, Vladan and Radonjić, Anda and Kostić, Igor",
year = "2019",
abstract = "The bean weevil (Acanthoscelides obtectus, Say) is a serious pest of stored bean seeds. Bean weevil control relies heavily on the use of synthetic insecticides. In the search for a sustainable alternative, the residual contact toxicity and anti-oviposition activity of thyme (Thymus vulgaris L.), rosemary (Rosmarinus officinalis L.) and basil (Ocimum basilicum L.) essential oils as well as their dominant components (thymol, α-pinene, 1,8-cineole and linalool) were tested against A. obtectus adults. Out of the seven tested botanicals, T. vulgaris oil, thymol and linalool exhibited the highest toxic potential (>90% mortality). Females were less susceptible than males. The insecticidal activity of these botanicals was much greater when they were applied on glass compared to direct application to the bean. All tested botanicals reduced oviposition by bean weevil females. T. vulgaris oil, thymol and α-pinene also deterred bean weevil oviposition, as revealed by a two-choice test. Our research shows that T. vulgaris oil and thymol are promising and sustainable alternatives to synthetic pesticides for protecting stored beans against the bean weevil.",
journal = "Archives of Biological Sciences",
title = "Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans",
number = "4",
volume = "71",
doi = "10.2298/ABS190617049J",
pages = "665-676"
}

Jevremović, S., Lazarević, J., Kostić, M., Krnjajić, S., Ugrenović, V., Radonjić, A.,& Kostić, I.. (2019). Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences, 71(4), 665-676.
https://doi.org/10.2298/ABS190617049J

Jevremović S, Lazarević J, Kostić M, Krnjajić S, Ugrenović V, Radonjić A, Kostić I. Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans. in Archives of Biological Sciences. 2019;71(4):665-676.
doi:10.2298/ABS190617049J .

Jevremović, Stojan, Lazarević, Jelica, Kostić, Miroslav, Krnjajić, Slobodan, Ugrenović, Vladan, Radonjić, Anda, Kostić, Igor, "Contact application of lamiaceae botanicals reduces bean weevil infestation in stored beans" in Archives of Biological Sciences, 71, no. 4 (2019):665-676,
https://doi.org/10.2298/ABS190617049J . .

TY  - CONF
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Kostić, Miroslav
AU  - Savić, Jelena
AU  - Belić, Maja
AU  - Ćosić, Tatjana
AU  - Ćalić, Dušica
PY  - 2018
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4326
AB  - Larvae of gypsy moth (Lymantria dispar L.) are major defoliators of deciduous forests and urban
environment. The biological means of regulation of moth population density were incorporated
along with conventional treatment in the integrative management concept. The effects of tansy
(Tanacetum vulgare L.) essential oil (EO) to gypsy moths were monitored through the effects of
residual contact and digestive toxicity on the mortality and development in the second instar
larvae, but also through the growth and feeding indices of the fourth instar larvae. Although EO
presence didn’t cause residual contact toxicity or significantly affect larval mortality, tested oil notably
elongated developmental time by delaying the molting moment and reduced percentage
of molted larvae. On the other hand, EO digestion caused significant mortality in the second instar
larvae (72% compared to control, at the end of experiment). Digestion of food enriched with tansy
oil decreased the efficiency of the diet, and led to reduced mass gain, and therefore induced the
delay of the molting moment, or completely stopped molting into the third instar larvae. Besides
that, EO reduced the daily mass gain (0.06 mg mg-1 day-1 compared to 0.3 mg mg-1 day-1 in control)
and the food consumption rate (0.61 mg mg-1 day-1 compared to 1.23 mg mg-1 day-1 in control)
in the fourth instar larvae and caused decrease of the conversion rate of digested food to larvae
body mass (10.62% compared to 26.05% in control). Despite that, food assimilation rate to larvae
body mass was not significantly decreased, which moderated negative effects of EO digestion as
well as reduced food intake.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
T1  - Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae
SP  - 136
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4326
ER  - 

@conference{
author = "Devrnja, Nina and Kostić, Igor and Lazarević, Jelica and Kostić, Miroslav and Savić, Jelena and Belić, Maja and Ćosić, Tatjana and Ćalić, Dušica",
year = "2018",
abstract = "Larvae of gypsy moth (Lymantria dispar L.) are major defoliators of deciduous forests and urban
environment. The biological means of regulation of moth population density were incorporated
along with conventional treatment in the integrative management concept. The effects of tansy
(Tanacetum vulgare L.) essential oil (EO) to gypsy moths were monitored through the effects of
residual contact and digestive toxicity on the mortality and development in the second instar
larvae, but also through the growth and feeding indices of the fourth instar larvae. Although EO
presence didn’t cause residual contact toxicity or significantly affect larval mortality, tested oil notably
elongated developmental time by delaying the molting moment and reduced percentage
of molted larvae. On the other hand, EO digestion caused significant mortality in the second instar
larvae (72% compared to control, at the end of experiment). Digestion of food enriched with tansy
oil decreased the efficiency of the diet, and led to reduced mass gain, and therefore induced the
delay of the molting moment, or completely stopped molting into the third instar larvae. Besides
that, EO reduced the daily mass gain (0.06 mg mg-1 day-1 compared to 0.3 mg mg-1 day-1 in control)
and the food consumption rate (0.61 mg mg-1 day-1 compared to 1.23 mg mg-1 day-1 in control)
in the fourth instar larvae and caused decrease of the conversion rate of digested food to larvae
body mass (10.62% compared to 26.05% in control). Despite that, food assimilation rate to larvae
body mass was not significantly decreased, which moderated negative effects of EO digestion as
well as reduced food intake.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts",
title = "Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae",
pages = "136",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4326"
}

Devrnja, N., Kostić, I., Lazarević, J., Kostić, M., Savić, J., Belić, M., Ćosić, T.,& Ćalić, D.. (2018). Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts
Belgrade: Serbian Plant Physiology Society., 136.
https://hdl.handle.net/21.15107/rcub_ibiss_4326

Devrnja N, Kostić I, Lazarević J, Kostić M, Savić J, Belić M, Ćosić T, Ćalić D. Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae. in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts. 2018;:136.
https://hdl.handle.net/21.15107/rcub_ibiss_4326 .

Devrnja, Nina, Kostić, Igor, Lazarević, Jelica, Kostić, Miroslav, Savić, Jelena, Belić, Maja, Ćosić, Tatjana, Ćalić, Dušica, "Effects of tansy essential oil on fitness and digestion process of gypsy moth larvae" in 3rd International Conference on Plant Biology and 22nd Symposium of the Serbian Plant Physiology Society, Belgrade, Serbia, Book of abstracts (2018):136,
https://hdl.handle.net/21.15107/rcub_ibiss_4326 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Radojković, Aleksandar
AU  - Kostić, Igor
AU  - Krnjajić, Slobodan
AU  - Mitrović, Jelena
AU  - Kostić, Miroslav B.
AU  - Novaković, Tatjana
AU  - Branković, Zorica
AU  - Branković, Goran
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0022474X17304083?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3051
AB  - For long-term protection of stored products there is a growing demand to replace chemical insecticides due to their effects on human health and environmental safety. Particulate materials, such as inert dusts and various submicron and nanomaterials have been extensively tested as viable alternatives. This is the first study on the insecticidal impact of alumina powder (α-Al2O3) on the bruchid pest, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae: Bruchinae). By altering the fuel to oxidant molar ratio (F/O: 0.5, 0.8, 1.2) in an autocombustion reaction we synthesized alumina powders with specific surface area and particle size varying from the nano- to micron scale. It was found that particle morphology influenced survival and progeny number of A. obtectus. The order of powders from low to high efficacy in reducing beetle performance (F/O-0.8 < F/O-0.5 < F/O-1.2) correlated well with increase in surface area, pore volume and diameter, and decrease in particle size. Survival was also affected by time of exposure, the applied dose and sex. The estimated median lethal concentration of the most efficient powder F/O-1.2 was significantly lower in males (LC50 = 330.4 ppm) than in females (LC50 = 409.6 ppm). Our results suggest that alumina powder can be considered for seed protection against A. obtectus, particularly during long-term storage, as it is cost effective, exerts limited toxicity to humans and demands no repetitive use like conventional pesticides.
T2  - Journal of Stored Products Research
T1  - Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)
VL  - 77
DO  - 10.1016/J.JSPR.2018.02.006
SP  - 45
EP  - 54
ER  - 

@article{
author = "Lazarević, Jelica and Radojković, Aleksandar and Kostić, Igor and Krnjajić, Slobodan and Mitrović, Jelena and Kostić, Miroslav B. and Novaković, Tatjana and Branković, Zorica and Branković, Goran",
year = "2018",
abstract = "For long-term protection of stored products there is a growing demand to replace chemical insecticides due to their effects on human health and environmental safety. Particulate materials, such as inert dusts and various submicron and nanomaterials have been extensively tested as viable alternatives. This is the first study on the insecticidal impact of alumina powder (α-Al2O3) on the bruchid pest, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae: Bruchinae). By altering the fuel to oxidant molar ratio (F/O: 0.5, 0.8, 1.2) in an autocombustion reaction we synthesized alumina powders with specific surface area and particle size varying from the nano- to micron scale. It was found that particle morphology influenced survival and progeny number of A. obtectus. The order of powders from low to high efficacy in reducing beetle performance (F/O-0.8 < F/O-0.5 < F/O-1.2) correlated well with increase in surface area, pore volume and diameter, and decrease in particle size. Survival was also affected by time of exposure, the applied dose and sex. The estimated median lethal concentration of the most efficient powder F/O-1.2 was significantly lower in males (LC50 = 330.4 ppm) than in females (LC50 = 409.6 ppm). Our results suggest that alumina powder can be considered for seed protection against A. obtectus, particularly during long-term storage, as it is cost effective, exerts limited toxicity to humans and demands no repetitive use like conventional pesticides.",
journal = "Journal of Stored Products Research",
title = "Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)",
volume = "77",
doi = "10.1016/J.JSPR.2018.02.006",
pages = "45-54"
}

Lazarević, J., Radojković, A., Kostić, I., Krnjajić, S., Mitrović, J., Kostić, M. B., Novaković, T., Branković, Z.,& Branković, G.. (2018). Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say). in Journal of Stored Products Research, 77, 45-54.
https://doi.org/10.1016/J.JSPR.2018.02.006

Lazarević J, Radojković A, Kostić I, Krnjajić S, Mitrović J, Kostić MB, Novaković T, Branković Z, Branković G. Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say). in Journal of Stored Products Research. 2018;77:45-54.
doi:10.1016/J.JSPR.2018.02.006 .

Lazarević, Jelica, Radojković, Aleksandar, Kostić, Igor, Krnjajić, Slobodan, Mitrović, Jelena, Kostić, Miroslav B., Novaković, Tatjana, Branković, Zorica, Branković, Goran, "Insecticidal impact of alumina powders against Acanthoscelides obtectus (Say)" in Journal of Stored Products Research, 77 (2018):45-54,
https://doi.org/10.1016/J.JSPR.2018.02.006 . .

TY  - JOUR
AU  - Toscano, Bruno
AU  - Štrbac, Pero
AU  - Popović, Zorica
AU  - Kostić, Miroslav
AU  - Kostić, Igor
AU  - Konjević, Aleksandra
AU  - Krnjajić, Slobodan
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6732
AB  - A faunistic study of the family Elateridae was carried out in Bačka, the north-western district of Vojvodina Province in Serbia, focusing on their preferred habitats and soil types. The survey included four locations with a total of 1059 ha, in which 35 species, belonging to 15 genera of Elateridae, were observed. The frequency of larval and adult forms was 58.94% and 67.40%, respectively, proving that wireworms are regular residents of the area. Regarding habitat preferences, it was shown that a majority of Elateridae species prefer open biotopes and wheat crop, while marsh soil, chernozem and alluvium were the preferred soil types of wireworms.
PB  - Belgrade: Institute of Pesticides and Environmental protection
T2  - Pesticidi i fitomedicina
T1  - A faunistic study of the family Elateridae in Bačka, Serbia
IS  - 3-4
VL  - 32
DO  - 10.2298/PIF1704181T
SP  - 181
EP  - 188
ER  - 

@article{
author = "Toscano, Bruno and Štrbac, Pero and Popović, Zorica and Kostić, Miroslav and Kostić, Igor and Konjević, Aleksandra and Krnjajić, Slobodan",
year = "2017",
abstract = "A faunistic study of the family Elateridae was carried out in Bačka, the north-western district of Vojvodina Province in Serbia, focusing on their preferred habitats and soil types. The survey included four locations with a total of 1059 ha, in which 35 species, belonging to 15 genera of Elateridae, were observed. The frequency of larval and adult forms was 58.94% and 67.40%, respectively, proving that wireworms are regular residents of the area. Regarding habitat preferences, it was shown that a majority of Elateridae species prefer open biotopes and wheat crop, while marsh soil, chernozem and alluvium were the preferred soil types of wireworms.",
publisher = "Belgrade: Institute of Pesticides and Environmental protection",
journal = "Pesticidi i fitomedicina",
title = "A faunistic study of the family Elateridae in Bačka, Serbia",
number = "3-4",
volume = "32",
doi = "10.2298/PIF1704181T",
pages = "181-188"
}

Toscano, B., Štrbac, P., Popović, Z., Kostić, M., Kostić, I., Konjević, A.,& Krnjajić, S.. (2017). A faunistic study of the family Elateridae in Bačka, Serbia. in Pesticidi i fitomedicina
Belgrade: Institute of Pesticides and Environmental protection., 32(3-4), 181-188.
https://doi.org/10.2298/PIF1704181T

Toscano B, Štrbac P, Popović Z, Kostić M, Kostić I, Konjević A, Krnjajić S. A faunistic study of the family Elateridae in Bačka, Serbia. in Pesticidi i fitomedicina. 2017;32(3-4):181-188.
doi:10.2298/PIF1704181T .

Toscano, Bruno, Štrbac, Pero, Popović, Zorica, Kostić, Miroslav, Kostić, Igor, Konjević, Aleksandra, Krnjajić, Slobodan, "A faunistic study of the family Elateridae in Bačka, Serbia" in Pesticidi i fitomedicina, 32, no. 3-4 (2017):181-188,
https://doi.org/10.2298/PIF1704181T . .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Janković Tomanić, Milena
AU  - Kostić, Igor
AU  - Kostić, Miroslav
AU  - Morina, Filis
AU  - Živanović, Bojana
AU  - Lazarević, Jelica
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3956
AB  - Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, fre-
quently encounter spatial and temporal variations in diet quality. Such variation favoured the evolu-
tion of high behavioural and physiological plasticity which, depending on forest stand composition,
enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable
habitat, interspecific and intraspecific host quality variation may provoke significant variation in
gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently
explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches
between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.)
(TH and HT groups), under controlled laboratory conditions, and compared larval performance
between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian
oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak
quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hun-
garian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption
rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activ-
ities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT lar-
vae. It appeared that the lower growth in the TH group than in the TT group was caused by both
behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming
oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet
experience influenced the sensitivity of the most examined traits to less suitable Hungarian oaks, sug-
gesting the development of behavioural and physiological adjustments. Our results indicate that
lower risks of defoliation by gypsy moth might be expected in mixed stands with a higher proportion
of Hungarian oak.
PB  - The Netherlands Entomological Society
T2  - Entomologia Experimentalis et Applicata
T1  - Behavioural and physiological plasticity of gypsy moth larvae to host plant switching
IS  - 2
VL  - 158
DO  - 10.1111/eea.12388
SP  - 152
EP  - 162
ER  - 

@article{
author = "Milanović, Slobodan and Janković Tomanić, Milena and Kostić, Igor and Kostić, Miroslav and Morina, Filis and Živanović, Bojana and Lazarević, Jelica",
year = "2016",
abstract = "Larvae of the gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), a generalist species, fre-
quently encounter spatial and temporal variations in diet quality. Such variation favoured the evolu-
tion of high behavioural and physiological plasticity which, depending on forest stand composition,
enables more or less successful exploitation of the environment. Even in mixed oak stands, a suitable
habitat, interspecific and intraspecific host quality variation may provoke significant variation in
gypsy moth performance and, consequently, defoliation severity. To elucidate the insufficiently
explored relationship between gypsy moth and oaks (Fagaceae), we carried out reciprocal switches
between Turkey oaks (Quercus cerris L.) and less nutritious Hungarian oaks (Quercus frainetto Ten.)
(TH and HT groups), under controlled laboratory conditions, and compared larval performance
between the switched larvae and larvae continuously fed on either Turkey oak (TT) or Hungarian
oak (HH). We found that larval traits were most strongly affected by among-tree variation in oak
quality and identity of the host consumed during the fourth instar. Switching from Turkey to Hun-
garian oak (TH) led to a longer period of feeding, decrease of mass gain, growth, and consumption
rate, lower efficiency of food use and nutrient conversion, and increase of protease and amylase activ-
ities. Larvae exposed to the reverse switch (HT) attained values of these traits characteristic for TT lar-
vae. It appeared that the lower growth in the TH group than in the TT group was caused by both
behavioural (consumption, pre-ingestive) and metabolic (post-digestive) effects from consuming
oaks. Multivariate analyses of growth, consumption, and efficiency of food use revealed that early diet
experience influenced the sensitivity of the most examined traits to less suitable Hungarian oaks, sug-
gesting the development of behavioural and physiological adjustments. Our results indicate that
lower risks of defoliation by gypsy moth might be expected in mixed stands with a higher proportion
of Hungarian oak.",
publisher = "The Netherlands Entomological Society",
journal = "Entomologia Experimentalis et Applicata",
title = "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching",
number = "2",
volume = "158",
doi = "10.1111/eea.12388",
pages = "152-162"
}

Milanović, S., Janković Tomanić, M., Kostić, I., Kostić, M., Morina, F., Živanović, B.,& Lazarević, J.. (2016). Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis et Applicata
The Netherlands Entomological Society., 158(2), 152-162.
https://doi.org/10.1111/eea.12388

Milanović S, Janković Tomanić M, Kostić I, Kostić M, Morina F, Živanović B, Lazarević J. Behavioural and physiological plasticity of gypsy moth larvae to host plant switching. in Entomologia Experimentalis et Applicata. 2016;158(2):152-162.
doi:10.1111/eea.12388 .

Milanović, Slobodan, Janković Tomanić, Milena, Kostić, Igor, Kostić, Miroslav, Morina, Filis, Živanović, Bojana, Lazarević, Jelica, "Behavioural and physiological plasticity of gypsy moth larvae to host plant switching" in Entomologia Experimentalis et Applicata, 158, no. 2 (2016):152-162,
https://doi.org/10.1111/eea.12388 . .

TY  - JOUR
AU  - Toscano, Bruno
AU  - Štrbac, Pero
AU  - Popović, Zorica
AU  - Kostić, Miroslav
AU  - Kostić, Igor
AU  - Krnjajić, Slobodan
PY  - 2015
UR  - https://www.agrojournal.org/21/03-12.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6766
AB  - A survey was conducted on Elateridae fauna of Srem region, in the south of Vojvodina Province (Serbia) during 2010-2012. As many as 1202 specimens (666 adults and 536 larval forms) were collected from 949 ha on four localities and different types of habitats (crops and open biotops). Totally, 37 species belonging to 15 genera were identified. The most frequently present genus was Agroites, with the species A. ustulaus.
PB  - National Centre for Agrarian Sciences
T2  - Bulgarian Journal of Agricultural Science
T1  - Faunistic inventory of click beetles in srem region (vojvodina province, serbia)
IS  - 3
VL  - 21
SP  - 545
EP  - 553
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6766
ER  - 

@article{
author = "Toscano, Bruno and Štrbac, Pero and Popović, Zorica and Kostić, Miroslav and Kostić, Igor and Krnjajić, Slobodan",
year = "2015",
abstract = "A survey was conducted on Elateridae fauna of Srem region, in the south of Vojvodina Province (Serbia) during 2010-2012. As many as 1202 specimens (666 adults and 536 larval forms) were collected from 949 ha on four localities and different types of habitats (crops and open biotops). Totally, 37 species belonging to 15 genera were identified. The most frequently present genus was Agroites, with the species A. ustulaus.",
publisher = "National Centre for Agrarian Sciences",
journal = "Bulgarian Journal of Agricultural Science",
title = "Faunistic inventory of click beetles in srem region (vojvodina province, serbia)",
number = "3",
volume = "21",
pages = "545-553",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6766"
}

Toscano, B., Štrbac, P., Popović, Z., Kostić, M., Kostić, I.,& Krnjajić, S.. (2015). Faunistic inventory of click beetles in srem region (vojvodina province, serbia). in Bulgarian Journal of Agricultural Science
National Centre for Agrarian Sciences., 21(3), 545-553.
https://hdl.handle.net/21.15107/rcub_ibiss_6766

Toscano B, Štrbac P, Popović Z, Kostić M, Kostić I, Krnjajić S. Faunistic inventory of click beetles in srem region (vojvodina province, serbia). in Bulgarian Journal of Agricultural Science. 2015;21(3):545-553.
https://hdl.handle.net/21.15107/rcub_ibiss_6766 .

Toscano, Bruno, Štrbac, Pero, Popović, Zorica, Kostić, Miroslav, Kostić, Igor, Krnjajić, Slobodan, "Faunistic inventory of click beetles in srem region (vojvodina province, serbia)" in Bulgarian Journal of Agricultural Science, 21, no. 3 (2015):545-553,
https://hdl.handle.net/21.15107/rcub_ibiss_6766 .

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Kostić, Miroslav B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1009
AB  - The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG. (C) 2013 Elsevier B.V. All rights reserved.
T2  - Scientia Horticulturae
T1  - Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca
IS  - null
VL  - 155
SP  - 69
EP  - 122
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1009
ER  - 

@article{
author = "Ćalić, Dušica and Devrnja, Nina and Kostić, Igor and Kostić, Miroslav B",
year = "2013",
abstract = "The pollen morphology and exine ornamentation of an important autochthon plum cultivar (Prunus domestica cv. Pozegaca L), which has horticultural, medicinal and alimentary uses, was examined in detail using both light and scanning electron microscope (SEM). Acetocarmine and fluorescein diacetate were used to assess the pollen viability of four Pozegaca plum genotypes. Pollen viability varied from 67% in genotype Pdp1 to 99% in genotype Pdp4. Also, pollen nucleus status was determined, and binucleate mature pollens were detected. The effect of polyethylene glycol-PEG (10, 15 and 20%, w/v) on pollen germination and tube growth was evaluated. Overall, the inclusion of PEG in the medium improved both pollen germination and tube growth. Regardless of the PEG concentration genotype has an effect on germination and length of pollen tubes. Genotype has a pronounced effect on germination and length of pollen tubes, regardless of the PEG concentration used. Genotype Pdp4 had the highest germination percentage and the highest pollen tube length on all media when compared to the other three genotypes. The highest pollen germination (96%) and a tube length (822 mu m) of genotype PdP4 was attained in media with 20% PEG. (C) 2013 Elsevier B.V. All rights reserved.",
journal = "Scientia Horticulturae",
title = "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca",
number = "null",
volume = "155",
pages = "69-122",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1009"
}

Ćalić, D., Devrnja, N., Kostić, I.,& Kostić, M. B.. (2013). Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae, 155(null), 69-122.
https://hdl.handle.net/21.15107/rcub_ibiss_1009

Ćalić D, Devrnja N, Kostić I, Kostić MB. Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca. in Scientia Horticulturae. 2013;155(null):69-122.
https://hdl.handle.net/21.15107/rcub_ibiss_1009 .

Ćalić, Dušica, Devrnja, Nina, Kostić, Igor, Kostić, Miroslav B, "Pollen morphology, viability, and germination of Prunus domestica cv. Pozegaca" in Scientia Horticulturae, 155, no. null (2013):69-122,
https://hdl.handle.net/21.15107/rcub_ibiss_1009 .

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Bohanec, Borut
AU  - Devrnja, Nina
AU  - Milojević, Jelena
AU  - Tubić, Ljiljana B
AU  - Kostić, Igor
AU  - Zdravković-Korać, Snežana
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1006
AB  - Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.
T2  - Trees-Structure and Function
T1  - Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos
IS  - 3
VL  - 27
SP  - 99
EP  - 762
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1006
ER  - 

@article{
author = "Ćalić, Dušica and Bohanec, Borut and Devrnja, Nina and Milojević, Jelena and Tubić, Ljiljana B and Kostić, Igor and Zdravković-Korać, Snežana",
year = "2013",
abstract = "Horse chestnut (Aesculus hyppocastanum L., Hyppocastanacea) is a relict species with a slow and complex reproductive cycle considered to have horticultural and medical importance. The cycle maybe circumvented via in vitro androgenesis. Androgenesis of horse chestnut was induced in microspores and anther culture on MS media. Some of the horse chestnut androgenic embryos were albinos. Addition of abscisic acid in media (in concentrations of 0.01, 0.1, 0.5, 1, 2, 5, 10, and 20 mg l(-1)) with horse chestnut androgenic embryos has circumvented the reproduction cycle barriers. The best results were achieved on medium with the lowest abscisic acid concentration (0.01 mg l(-1)) in microspore culture. The microspore culture proved to be a better model system for embryo production and albino embryo reduction than anther culture. Flow cytometry analysis after maturation treatments induced by ABA showed that 88 % of green embryos originating from microspore culture were haploid. However, 50 % of green embryos from anther culture were haploid. The remaining analyzed androgenic embryos, from both types of cultures were diploid.",
journal = "Trees-Structure and Function",
title = "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos",
number = "3",
volume = "27",
pages = "99-762",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1006"
}

Ćalić, D., Bohanec, B., Devrnja, N., Milojević, J., Tubić, L. B., Kostić, I.,& Zdravković-Korać, S.. (2013). Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function, 27(3), 99-762.
https://hdl.handle.net/21.15107/rcub_ibiss_1006

Ćalić D, Bohanec B, Devrnja N, Milojević J, Tubić LB, Kostić I, Zdravković-Korać S. Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos. in Trees-Structure and Function. 2013;27(3):99-762.
https://hdl.handle.net/21.15107/rcub_ibiss_1006 .

Ćalić, Dušica, Bohanec, Borut, Devrnja, Nina, Milojević, Jelena, Tubić, Ljiljana B, Kostić, Igor, Zdravković-Korać, Snežana, "Impact of abscisic acid in overcoming the problem of albinism in horse chestnut androgenic embryos" in Trees-Structure and Function, 27, no. 3 (2013):99-762,
https://hdl.handle.net/21.15107/rcub_ibiss_1006 .

TY  - CONF
AU  - Zdravković-Korać, Snežana
AU  - Tubić, Ljiljana
AU  - Milojević, Jelena
AU  - Devrnja, Nina
AU  - Kostić, Igor
AU  - Ćalić, Dušica
PY  - 2012
UR  - http://www.forest.org.rs/pdf/proceedings-conference2012.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6124
AB  - Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.
PB  - Belgrade: Institute of Forestry
C3  - Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.
T1  - Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots
SP  - 389
EP  - 396
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6124
ER  - 

@conference{
author = "Zdravković-Korać, Snežana and Tubić, Ljiljana and Milojević, Jelena and Devrnja, Nina and Kostić, Igor and Ćalić, Dušica",
year = "2012",
abstract = "Efficient bud regeneration was achieved from germinating horse chestnut (Aesculus hippocastanum L.) somatic embryos cultivated on 1-10 μM benzyladenine (BA). Adventitious buds were detached from the mother tissue and used to establish permanent shoot cultures on 0-20 μM BA. Secondary buds were regenerated from the shoot base of the explants. Bud multiplication was very poor (1.9) and shoot-tip necrosis was very high (100%) on plant growth regulator (PGR)-free medium. The highest multiplication was achieved on 5 and 10 μM BA (16.8 and 18.7, respectively), with no shoot-tip necrosis, while hyperhydration was rather frequent on shoots cultivated on BA above 5 μM. Individual shoots were elongated on medium with 1 μM BA and 500 mg/l polyvinylpyrrolidone (PVP MW40 000) for 4 weeks. However, it was necessary to reduce BA level below 1 μM for shoot rooting and that caused mass shoot-tip necrosis. As classical rooting methods failed, the basal part of each elongated shoot was first wounded by cutting with a sterile blade and then dipped into a 0, 5 or 10 mM indole-3-butyric acid solution for 1 min and cultivated on solid half-strength MS PGR-free medium with 0.02% activated charcoal for 2-3 weeks. To prevent shoot tip necrosis during this phase, a BA solution was applied directly on apical meristem. Shoot-tip necrosis was completely eliminated by weekly application of 10 μl of 1 μM BA. As soon as the root initials were observed, the shoots were transferred to MS medium supplemented with 500 mg/l PVP and 5 μM BA. The frequency of rooting was 23%, and further optimisation of root-inducing phase is needed.",
publisher = "Belgrade: Institute of Forestry",
journal = "Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.",
title = "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots",
pages = "389-396",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6124"
}

Zdravković-Korać, S., Tubić, L., Milojević, J., Devrnja, N., Kostić, I.,& Ćalić, D.. (2012). Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.
Belgrade: Institute of Forestry., 389-396.
https://hdl.handle.net/21.15107/rcub_ibiss_6124

Zdravković-Korać S, Tubić L, Milojević J, Devrnja N, Kostić I, Ćalić D. Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots. in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia.. 2012;:389-396.
https://hdl.handle.net/21.15107/rcub_ibiss_6124 .

Zdravković-Korać, Snežana, Tubić, Ljiljana, Milojević, Jelena, Devrnja, Nina, Kostić, Igor, Ćalić, Dušica, "Rooting and preventing shoot-tip necrosis of in vitro cultured horse chestnut shoots" in Proceedings: International Scientific Conference: Forest in the Future - Sustainable Use, Risks and Challenges; 2012 Oct 4-5; Belgrade, Serbia. (2012):389-396,
https://hdl.handle.net/21.15107/rcub_ibiss_6124 .

TY  - JOUR
AU  - Ćalić, Dušica
AU  - Devrnja, Nina
AU  - Milojević, Jelena
AU  - Kostić, Igor
AU  - Janošević, Dušica A.
AU  - Budimir, Snežana
AU  - Zdravković-Korać, Snežana
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1070
AB  - The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.
T2  - Hortscience
T1  - Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis
IS  - 12
VL  - 47
SP  - 79
EP  - 1744
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1070
ER  - 

@article{
author = "Ćalić, Dušica and Devrnja, Nina and Milojević, Jelena and Kostić, Igor and Janošević, Dušica A. and Budimir, Snežana and Zdravković-Korać, Snežana",
year = "2012",
abstract = "The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg.L-1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg.L-1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.",
journal = "Hortscience",
title = "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis",
number = "12",
volume = "47",
pages = "79-1744",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1070"
}

Ćalić, D., Devrnja, N., Milojević, J., Kostić, I., Janošević, D. A., Budimir, S.,& Zdravković-Korać, S.. (2012). Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience, 47(12), 79-1744.
https://hdl.handle.net/21.15107/rcub_ibiss_1070

Ćalić D, Devrnja N, Milojević J, Kostić I, Janošević DA, Budimir S, Zdravković-Korać S. Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis. in Hortscience. 2012;47(12):79-1744.
https://hdl.handle.net/21.15107/rcub_ibiss_1070 .

Ćalić, Dušica, Devrnja, Nina, Milojević, Jelena, Kostić, Igor, Janošević, Dušica A., Budimir, Snežana, Zdravković-Korać, Snežana, "Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis" in Hortscience, 47, no. 12 (2012):79-1744,
https://hdl.handle.net/21.15107/rcub_ibiss_1070 .