TY  - JOUR
AU  - Santoro, Azzurra
AU  - Santolamazza, Federica
AU  - Cacciò, Simone
AU  - La Rosa, Giuseppina
AU  - Antolová, Daniela
AU  - Auer, Herbert
AU  - Bagrade, Guna
AU  - Bandelj, Petra
AU  - Basso, Walter
AU  - Beck, Relja
AU  - Citterio, Carlo
AU  - Davidson, Rebecca
AU  - Deksne, Gunita
AU  - Frey, Caroline
AU  - Fuglei, Eva
AU  - Glawischnig, Walter
AU  - Gottstein, Bruno
AU  - Harna, Jiri
AU  - Petersen, Heidi
AU  - Karamon, Jacek
AU  - Jansen, Famke
AU  - Jarošová, Júlia
AU  - Jokelainen, Pikka
AU  - Lundström-Stadelmann, Britta
AU  - Maksimov, Pavlo
AU  - Miljević, Milan
AU  - Miterpáková, Martina
AU  - Moks, Epp
AU  - Origgi, Francesco
AU  - Ozolina, Zanda
AU  - Ryser, Marie-Pierre
AU  - Romig, Thomas
AU  - Šarkūnas, Mindaugas
AU  - Scorrano, Nathalie
AU  - Saarma, Urmas
AU  - Šnábel, Viliam
AU  - Sréter, Tamas
AU  - Umhang, Gerald
AU  - Vengušt, Gorzad
AU  - Žele Vengušt, Diana
AU  - Casulli, Adriano
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6523
AB  - The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a fatal zoonotic parasitic disease of the northern hemisphere. Red foxes are the main reservoir hosts and, likely, the main drivers of the geographic spread of the disease in Europe. Knowledge of genetic relationships among E. multilocularis isolates at a European scale is key to understanding the dispersal characteristics of E. multilocularis. Hence, the present study aimed to describe the genetic diversity of E. multilocularis isolates obtained from different host species in 19 European countries. Based on the analysis of complete nucleotide sequences of the cob, atp6, nad2, nad1 and cox1 mitochondrial genes (4,968 bp), 43 haplotypes were inferred. Four haplotypes represented 62.56 % of the examined isolates (142/227), and one of these four haplotypes was found in each country investigated, except Svalbard, Norway. While the haplotypes from Svalbard were markedly different from all the others, mainland Europe appeared to be dominated by two main clusters, represented by most western, central and eastern European countries, and the Baltic countries and northeastern Poland, respectively. Moreover, one Asian-like haplotype was identified in Latvia and northeastern Poland. To better elucidate the presence of Asian genetic variants of E. multilocularis in Europe, and to obtain a more comprehensive Europe-wide coverage, further studies, including samples from endemic regions not investigated in the present study, especially some eastern European countries, are needed. Further, the present work proposes historical causes that may have contributed to shaping the current genetic variability of E. multilocularis in Europe
PB  - Elsevier Ltd on behalf of Australian Society for Parasitology
T2  - International Journal for Parasitology
T1  - Mitochondrial genetic diversity and phylogenetic relationships of Echinococcus multilocularis in Europe
DO  - 10.1016/j.ijpara.2024.01.003
ER  - 

@article{
author = "Santoro, Azzurra and Santolamazza, Federica and Cacciò, Simone and La Rosa, Giuseppina and Antolová, Daniela and Auer, Herbert and Bagrade, Guna and Bandelj, Petra and Basso, Walter and Beck, Relja and Citterio, Carlo and Davidson, Rebecca and Deksne, Gunita and Frey, Caroline and Fuglei, Eva and Glawischnig, Walter and Gottstein, Bruno and Harna, Jiri and Petersen, Heidi and Karamon, Jacek and Jansen, Famke and Jarošová, Júlia and Jokelainen, Pikka and Lundström-Stadelmann, Britta and Maksimov, Pavlo and Miljević, Milan and Miterpáková, Martina and Moks, Epp and Origgi, Francesco and Ozolina, Zanda and Ryser, Marie-Pierre and Romig, Thomas and Šarkūnas, Mindaugas and Scorrano, Nathalie and Saarma, Urmas and Šnábel, Viliam and Sréter, Tamas and Umhang, Gerald and Vengušt, Gorzad and Žele Vengušt, Diana and Casulli, Adriano",
year = "2024",
abstract = "The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a fatal zoonotic parasitic disease of the northern hemisphere. Red foxes are the main reservoir hosts and, likely, the main drivers of the geographic spread of the disease in Europe. Knowledge of genetic relationships among E. multilocularis isolates at a European scale is key to understanding the dispersal characteristics of E. multilocularis. Hence, the present study aimed to describe the genetic diversity of E. multilocularis isolates obtained from different host species in 19 European countries. Based on the analysis of complete nucleotide sequences of the cob, atp6, nad2, nad1 and cox1 mitochondrial genes (4,968 bp), 43 haplotypes were inferred. Four haplotypes represented 62.56 % of the examined isolates (142/227), and one of these four haplotypes was found in each country investigated, except Svalbard, Norway. While the haplotypes from Svalbard were markedly different from all the others, mainland Europe appeared to be dominated by two main clusters, represented by most western, central and eastern European countries, and the Baltic countries and northeastern Poland, respectively. Moreover, one Asian-like haplotype was identified in Latvia and northeastern Poland. To better elucidate the presence of Asian genetic variants of E. multilocularis in Europe, and to obtain a more comprehensive Europe-wide coverage, further studies, including samples from endemic regions not investigated in the present study, especially some eastern European countries, are needed. Further, the present work proposes historical causes that may have contributed to shaping the current genetic variability of E. multilocularis in Europe",
publisher = "Elsevier Ltd on behalf of Australian Society for Parasitology",
journal = "International Journal for Parasitology",
title = "Mitochondrial genetic diversity and phylogenetic relationships of Echinococcus multilocularis in Europe",
doi = "10.1016/j.ijpara.2024.01.003"
}

Santoro, A., Santolamazza, F., Cacciò, S., La Rosa, G., Antolová, D., Auer, H., Bagrade, G., Bandelj, P., Basso, W., Beck, R., Citterio, C., Davidson, R., Deksne, G., Frey, C., Fuglei, E., Glawischnig, W., Gottstein, B., Harna, J., Petersen, H., Karamon, J., Jansen, F., Jarošová, J., Jokelainen, P., Lundström-Stadelmann, B., Maksimov, P., Miljević, M., Miterpáková, M., Moks, E., Origgi, F., Ozolina, Z., Ryser, M., Romig, T., Šarkūnas, M., Scorrano, N., Saarma, U., Šnábel, V., Sréter, T., Umhang, G., Vengušt, G., Žele Vengušt, D.,& Casulli, A.. (2024). Mitochondrial genetic diversity and phylogenetic relationships of Echinococcus multilocularis in Europe. in International Journal for Parasitology
Elsevier Ltd on behalf of Australian Society for Parasitology..
https://doi.org/10.1016/j.ijpara.2024.01.003

Santoro A, Santolamazza F, Cacciò S, La Rosa G, Antolová D, Auer H, Bagrade G, Bandelj P, Basso W, Beck R, Citterio C, Davidson R, Deksne G, Frey C, Fuglei E, Glawischnig W, Gottstein B, Harna J, Petersen H, Karamon J, Jansen F, Jarošová J, Jokelainen P, Lundström-Stadelmann B, Maksimov P, Miljević M, Miterpáková M, Moks E, Origgi F, Ozolina Z, Ryser M, Romig T, Šarkūnas M, Scorrano N, Saarma U, Šnábel V, Sréter T, Umhang G, Vengušt G, Žele Vengušt D, Casulli A. Mitochondrial genetic diversity and phylogenetic relationships of Echinococcus multilocularis in Europe. in International Journal for Parasitology. 2024;.
doi:10.1016/j.ijpara.2024.01.003 .

Santoro, Azzurra, Santolamazza, Federica, Cacciò, Simone, La Rosa, Giuseppina, Antolová, Daniela, Auer, Herbert, Bagrade, Guna, Bandelj, Petra, Basso, Walter, Beck, Relja, Citterio, Carlo, Davidson, Rebecca, Deksne, Gunita, Frey, Caroline, Fuglei, Eva, Glawischnig, Walter, Gottstein, Bruno, Harna, Jiri, Petersen, Heidi, Karamon, Jacek, Jansen, Famke, Jarošová, Júlia, Jokelainen, Pikka, Lundström-Stadelmann, Britta, Maksimov, Pavlo, Miljević, Milan, Miterpáková, Martina, Moks, Epp, Origgi, Francesco, Ozolina, Zanda, Ryser, Marie-Pierre, Romig, Thomas, Šarkūnas, Mindaugas, Scorrano, Nathalie, Saarma, Urmas, Šnábel, Viliam, Sréter, Tamas, Umhang, Gerald, Vengušt, Gorzad, Žele Vengušt, Diana, Casulli, Adriano, "Mitochondrial genetic diversity and phylogenetic relationships of Echinococcus multilocularis in Europe" in International Journal for Parasitology (2024),
https://doi.org/10.1016/j.ijpara.2024.01.003 . .

TY  - CONF
AU  - Santoro, Azzurra
AU  - Santolamazza, Federica
AU  - Cacciò, Simone
AU  - La Rosa, Giuseppina
AU  - Antolová, Daniela
AU  - Auer, Herbert
AU  - Bagrade, Guna
AU  - Bandelj, Petra
AU  - Basso, Walter
AU  - Beck, Relja
AU  - Citterio, Carlo Vittorio
AU  - Davidson, Rebecca
AU  - Deksne, Gunita
AU  - Frey, Caroline
AU  - Fuglei, Eva
AU  - Glawischnig, Walter
AU  - Gottstein, Bruno
AU  - Harna, Jiri
AU  - Petersen, Heidi
AU  - Karamon, Jacek
AU  - Jansen, Famke
AU  - Jarošová, Júlia
AU  - Jokelainen, Pikka
AU  - Lundström-Stadelmann, Britta
AU  - Maksimov, Pavlo
AU  - Miljević, Milan
AU  - Miterpáková, Martina
AU  - Moks, Epp
AU  - Origgi, Francesco
AU  - Ozolina, Zanda
AU  - Ryser, Marie-Pierre
AU  - Romig, Thomas
AU  - Šarkūnas, Mindaugas
AU  - Scorrano, Nathalie
AU  - Saarma, Urmas
AU  - Šnábel, Viliam
AU  - Sréter, Tamas
AU  - Umhang, Gerald
AU  - Vengušt, Gorzad
AU  - Žele Vengušt, Diana
AU  - Vergles Rataj, Aleksandra
AU  - Casulli, Adriano
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6406
AB  - The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a highly fatal zoonotic parasitic disease of the northern hemisphere [1]. Red foxes are the main reservoir hosts and, probably, the main drivers of the geographic spreading of the disease in Europe. Knowledge of genetic relationship among E. multilocularis isolates at European scale is a key to understand the dispersal characteristics of E. multilocularis [2,3]. Hence, the present study aimed to describe the genetic diversity of E. multilocularis isolates obtained from different hosts in 19 European countries. Based on the analysis of complete nucleotide sequences of the cob, atp6, nad2, nad1 and cox1 mitochondrial genes (4,968 bp), 43 haplotypes were inferred. Four haplotypes represented 62.56% of the examined isolates (142/227), and one of these four haplotypes was found in each country investigated, except Svalbard (Norway). While the haplotypes from Svalbard were markedly different from all the others, mainland Europe appeared to be dominated by two main clusters, represented by most western-central-eastern European countries, and the Baltic countries including North-East Poland, respectively. Further comparison was made with the cob, nad2 and cox1 Nakao’s historical haplotypes [4], unveiling the presence of one Asian-like haplotype identified in Latvia and North-Eastern Poland. To better elucidate the presence of Asian genetic variants of E. multilocularis in Europe, and to get a more comprehensive European wide coverage, further studies, including endemic regions not investigated in the present study, especially some Eastern European countries, are needed.
PB  - Bishkek: Kyrgyz State Medical Academy named after I.K. Akhunbaev
C3  - Collection of abstracts: 29th World Congress of Echinococcosis; 2023 Ag 28-30; Bishkek, Kyrgyzstan
T1  - The genetic diversity of echinococcus multilocularis in europe: a picture based on mitochondrial markers
SP  - 72
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6406
ER  - 

@conference{
author = "Santoro, Azzurra and Santolamazza, Federica and Cacciò, Simone and La Rosa, Giuseppina and Antolová, Daniela and Auer, Herbert and Bagrade, Guna and Bandelj, Petra and Basso, Walter and Beck, Relja and Citterio, Carlo Vittorio and Davidson, Rebecca and Deksne, Gunita and Frey, Caroline and Fuglei, Eva and Glawischnig, Walter and Gottstein, Bruno and Harna, Jiri and Petersen, Heidi and Karamon, Jacek and Jansen, Famke and Jarošová, Júlia and Jokelainen, Pikka and Lundström-Stadelmann, Britta and Maksimov, Pavlo and Miljević, Milan and Miterpáková, Martina and Moks, Epp and Origgi, Francesco and Ozolina, Zanda and Ryser, Marie-Pierre and Romig, Thomas and Šarkūnas, Mindaugas and Scorrano, Nathalie and Saarma, Urmas and Šnábel, Viliam and Sréter, Tamas and Umhang, Gerald and Vengušt, Gorzad and Žele Vengušt, Diana and Vergles Rataj, Aleksandra and Casulli, Adriano",
year = "2023",
abstract = "The cestode Echinococcus multilocularis is the causative agent of alveolar echinococcosis, a highly fatal zoonotic parasitic disease of the northern hemisphere [1]. Red foxes are the main reservoir hosts and, probably, the main drivers of the geographic spreading of the disease in Europe. Knowledge of genetic relationship among E. multilocularis isolates at European scale is a key to understand the dispersal characteristics of E. multilocularis [2,3]. Hence, the present study aimed to describe the genetic diversity of E. multilocularis isolates obtained from different hosts in 19 European countries. Based on the analysis of complete nucleotide sequences of the cob, atp6, nad2, nad1 and cox1 mitochondrial genes (4,968 bp), 43 haplotypes were inferred. Four haplotypes represented 62.56% of the examined isolates (142/227), and one of these four haplotypes was found in each country investigated, except Svalbard (Norway). While the haplotypes from Svalbard were markedly different from all the others, mainland Europe appeared to be dominated by two main clusters, represented by most western-central-eastern European countries, and the Baltic countries including North-East Poland, respectively. Further comparison was made with the cob, nad2 and cox1 Nakao’s historical haplotypes [4], unveiling the presence of one Asian-like haplotype identified in Latvia and North-Eastern Poland. To better elucidate the presence of Asian genetic variants of E. multilocularis in Europe, and to get a more comprehensive European wide coverage, further studies, including endemic regions not investigated in the present study, especially some Eastern European countries, are needed.",
publisher = "Bishkek: Kyrgyz State Medical Academy named after I.K. Akhunbaev",
journal = "Collection of abstracts: 29th World Congress of Echinococcosis; 2023 Ag 28-30; Bishkek, Kyrgyzstan",
title = "The genetic diversity of echinococcus multilocularis in europe: a picture based on mitochondrial markers",
pages = "72-73",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6406"
}

Santoro, A., Santolamazza, F., Cacciò, S., La Rosa, G., Antolová, D., Auer, H., Bagrade, G., Bandelj, P., Basso, W., Beck, R., Citterio, C. V., Davidson, R., Deksne, G., Frey, C., Fuglei, E., Glawischnig, W., Gottstein, B., Harna, J., Petersen, H., Karamon, J., Jansen, F., Jarošová, J., Jokelainen, P., Lundström-Stadelmann, B., Maksimov, P., Miljević, M., Miterpáková, M., Moks, E., Origgi, F., Ozolina, Z., Ryser, M., Romig, T., Šarkūnas, M., Scorrano, N., Saarma, U., Šnábel, V., Sréter, T., Umhang, G., Vengušt, G., Žele Vengušt, D., Vergles Rataj, A.,& Casulli, A.. (2023). The genetic diversity of echinococcus multilocularis in europe: a picture based on mitochondrial markers. in Collection of abstracts: 29th World Congress of Echinococcosis; 2023 Ag 28-30; Bishkek, Kyrgyzstan
Bishkek: Kyrgyz State Medical Academy named after I.K. Akhunbaev., 72-73.
https://hdl.handle.net/21.15107/rcub_ibiss_6406

Santoro A, Santolamazza F, Cacciò S, La Rosa G, Antolová D, Auer H, Bagrade G, Bandelj P, Basso W, Beck R, Citterio CV, Davidson R, Deksne G, Frey C, Fuglei E, Glawischnig W, Gottstein B, Harna J, Petersen H, Karamon J, Jansen F, Jarošová J, Jokelainen P, Lundström-Stadelmann B, Maksimov P, Miljević M, Miterpáková M, Moks E, Origgi F, Ozolina Z, Ryser M, Romig T, Šarkūnas M, Scorrano N, Saarma U, Šnábel V, Sréter T, Umhang G, Vengušt G, Žele Vengušt D, Vergles Rataj A, Casulli A. The genetic diversity of echinococcus multilocularis in europe: a picture based on mitochondrial markers. in Collection of abstracts: 29th World Congress of Echinococcosis; 2023 Ag 28-30; Bishkek, Kyrgyzstan. 2023;:72-73.
https://hdl.handle.net/21.15107/rcub_ibiss_6406 .

Santoro, Azzurra, Santolamazza, Federica, Cacciò, Simone, La Rosa, Giuseppina, Antolová, Daniela, Auer, Herbert, Bagrade, Guna, Bandelj, Petra, Basso, Walter, Beck, Relja, Citterio, Carlo Vittorio, Davidson, Rebecca, Deksne, Gunita, Frey, Caroline, Fuglei, Eva, Glawischnig, Walter, Gottstein, Bruno, Harna, Jiri, Petersen, Heidi, Karamon, Jacek, Jansen, Famke, Jarošová, Júlia, Jokelainen, Pikka, Lundström-Stadelmann, Britta, Maksimov, Pavlo, Miljević, Milan, Miterpáková, Martina, Moks, Epp, Origgi, Francesco, Ozolina, Zanda, Ryser, Marie-Pierre, Romig, Thomas, Šarkūnas, Mindaugas, Scorrano, Nathalie, Saarma, Urmas, Šnábel, Viliam, Sréter, Tamas, Umhang, Gerald, Vengušt, Gorzad, Žele Vengušt, Diana, Vergles Rataj, Aleksandra, Casulli, Adriano, "The genetic diversity of echinococcus multilocularis in europe: a picture based on mitochondrial markers" in Collection of abstracts: 29th World Congress of Echinococcosis; 2023 Ag 28-30; Bishkek, Kyrgyzstan (2023):72-73,
https://hdl.handle.net/21.15107/rcub_ibiss_6406 .

TY  - JOUR
AU  - Miljević, Milan
AU  - Rajičić, Marija
AU  - Umhang, Gerald
AU  - Bajić, Branka
AU  - Bjelić Čabrilo, Olivera
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6016
AB  - Background
Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu lato (s.l.) species is difficult without the use of molecular methods. The aim of this study was to identify and explore the genetic diversity of Hydatigera and other taeniid species.

Methods
Ten different small mammals species (856 individuals) (Rattus rattus, three Apodemus, three Arvicolinae and three Soricidae species) were examined from 2013 to 2023. Captured animals were visually examined for cysts and visible lesions. Two markers were used for amplification and sequencing: cox1 and 12S rDNA.

Results
Molecular analysis of cysts and visible lesions revealed four taeniid species: Hydatigera kamiyai, H. taeniaeformis sensu stricto (s.s.), Taenia martis and T. crassiceps. Hydatigera kamiyai was found in Apodemus flavicollis, A. agrarius, Microtus arvalis and Crocidrua leucodon, while H. taeniaeformis s.s. is registered in R. rattus. Hydatigera kamiyai cox1 sequences clustered with European populations and showed at least 25 nucleotid differences compared to Asian, African, Australian and one of our isolates of H. taeniaeformis s.s acquired from a rat, followed by large sequence distances (9.4% to 12.9%), indicating clear molecular distinction of two species.

Conclusions
This is one of the few mitochondrial gene-based studies performed after the description of cryptic entities within the Hydatigera taeniaeformis s.l. complex and represents a valuable contribution to understanding of genetic diversity, host suitability and geographic distribution of these tapeworm species. Also, our study provides an important basis of molecular data from this part of Europe for further studies. We emphasize the importance of additional studies of intermediate hosts, especially rats from Europe and Apodemus spp. and voles from Asia and Africa.
PB  - Springer Nature
T2  - Parasites and Vectors
T1  - Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia
IS  - 1
VL  - 16
DO  - 10.1186/s13071-023-05879-x
SP  - 250
ER  - 

@article{
author = "Miljević, Milan and Rajičić, Marija and Umhang, Gerald and Bajić, Branka and Bjelić Čabrilo, Olivera and Budinski, Ivana and Blagojević, Jelena",
year = "2023",
abstract = "Background
Hydatigera (Cestoda: Taeniidae) is a recently resurrected genus with the description of a new species, Hydatigera kamiyai, a cryptic entity within the Hydatigera taeniaeformis species complex. Rodents are intermediate hosts and correct taxonomic identification of H. taeniaeformis sensu lato (s.l.) species is difficult without the use of molecular methods. The aim of this study was to identify and explore the genetic diversity of Hydatigera and other taeniid species.

Methods
Ten different small mammals species (856 individuals) (Rattus rattus, three Apodemus, three Arvicolinae and three Soricidae species) were examined from 2013 to 2023. Captured animals were visually examined for cysts and visible lesions. Two markers were used for amplification and sequencing: cox1 and 12S rDNA.

Results
Molecular analysis of cysts and visible lesions revealed four taeniid species: Hydatigera kamiyai, H. taeniaeformis sensu stricto (s.s.), Taenia martis and T. crassiceps. Hydatigera kamiyai was found in Apodemus flavicollis, A. agrarius, Microtus arvalis and Crocidrua leucodon, while H. taeniaeformis s.s. is registered in R. rattus. Hydatigera kamiyai cox1 sequences clustered with European populations and showed at least 25 nucleotid differences compared to Asian, African, Australian and one of our isolates of H. taeniaeformis s.s acquired from a rat, followed by large sequence distances (9.4% to 12.9%), indicating clear molecular distinction of two species.

Conclusions
This is one of the few mitochondrial gene-based studies performed after the description of cryptic entities within the Hydatigera taeniaeformis s.l. complex and represents a valuable contribution to understanding of genetic diversity, host suitability and geographic distribution of these tapeworm species. Also, our study provides an important basis of molecular data from this part of Europe for further studies. We emphasize the importance of additional studies of intermediate hosts, especially rats from Europe and Apodemus spp. and voles from Asia and Africa.",
publisher = "Springer Nature",
journal = "Parasites and Vectors",
title = "Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia",
number = "1",
volume = "16",
doi = "10.1186/s13071-023-05879-x",
pages = "250"
}

Miljević, M., Rajičić, M., Umhang, G., Bajić, B., Bjelić Čabrilo, O., Budinski, I.,& Blagojević, J.. (2023). Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia. in Parasites and Vectors
Springer Nature., 16(1), 250.
https://doi.org/10.1186/s13071-023-05879-x

Miljević M, Rajičić M, Umhang G, Bajić B, Bjelić Čabrilo O, Budinski I, Blagojević J. Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia. in Parasites and Vectors. 2023;16(1):250.
doi:10.1186/s13071-023-05879-x .

Miljević, Milan, Rajičić, Marija, Umhang, Gerald, Bajić, Branka, Bjelić Čabrilo, Olivera, Budinski, Ivana, Blagojević, Jelena, "Cryptic species Hydatigera kamiyai and other taeniid metacestodes in the populations of small mammals in Serbia" in Parasites and Vectors, 16, no. 1 (2023):250,
https://doi.org/10.1186/s13071-023-05879-x . .

TY  - CONF
AU  - Miljević, Milan
AU  - Rajičić, Marija
AU  - Umhang, Gerald
AU  - Bjelić Čabrilo, Olivera
AU  - Bajić, Branka
AU  - Budinski, Ivana
AU  - Blagojević, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6033
AB  - The family Taeniidae recently consists of 4 genera, with a newly described cryptic species H. kamiyai within the Hydatigera genus. H. kamiyai is distributed across Europe to western Siberia, whereas H. taeniaeformis s.s. probably originated in Asia but has spread worldwide. Despite the extraordinary genetic divergence, the two species differ little morphologically, making it questionable whether morphological identification is even relevant in this case. Considering the geographical location of the studied area and the intermediate hosts targeted, we expected the presence of H. kamiyai, and our aim was to confirm this assumption by genetic analyses. From March to October 2013 to 2022, a total of 846 small mammals (Apodemus agrarius (152), Apodemus flavicollis (521), Apodemus sylvaticus (33), Microtus arvalis (48), Microtus subteraneus (17), Myodes glareolus (52), Crocidura suaveolens (13), Crocidura leucodon (7) and Sorex araneus (3)) were captured from 45 different localities in Serbia. Cysts and visible lesions in the body cavity and various organs were examined. Two markers (cox1 and 12S rDNA) were used for amplification and sequencing. A total of 21 strobilocercus of H. kamiyai were identified corresponding to thirteen sequences of the cox1 gene (318 bp) and eight of 12S rDNA (238 bp). H. kamiyai is found in 17 A. flavicollis, one A. agrarius, two M. arvalis, and one C. leucodon. According to the median-joining network, the 13 H. kamiyai cox1 sequences from this study clustered with isolates from Europe and western Siberia differing up to 2.7%. These results confirm and highlight the different geographic distribution and molecular differences in the Hydatigera genus. The results also confirm host suitability for this species. This is the first report of this newly described cryptic species in our country confirmed by genetic analysis.
AB  - Фамилија Taeniidae од недавно се састоји од 4 рода, са ново-описаном криптичном врстом Hydatigera kamiyai у оквиру рода Hydatigera. H. kamiyai врста је распрострањена широм Европе до западног Сибира, док H. taeniaeformis s.s вероватно потиче из Азије, али је распрострањена широм света. Упркос изузетној генетској дивергенцији, ове две врсте се мало разликују морфолошки, што доводи у питање да ли је морфолошка идентификација уопште релевантна у овом случају. С обзиром на географску локацију проучаваног подручја и циљане домаћине, очекивали смо присуство врсте H. kamiyai, са циљем да генетским анализама потврдимо ову претпоставку. Од марта до октобра 2013. до 2022., укупно 846 ситних сисара (Apodemus agrarius (152), Apodemus flavicollis (521), Apodemus sylvaticus (33), Microtus arvalis (48), Microtus subteraneus (17), Myodes glareolus (52), Crocidura suaveolens (13), Crocidura leucodon (7) и Sorex araneus (3)) прикупљено је са 45 различитих локалитета у Србији. Прегледане су цисте и видљиве лезије у телесној шупљини и разним органима. Два маркера (cox1 and 12S rDNA) коришћена су за амплификацију и секвенцирање. Идентификовано је укупно 21 strobilocercus H. kamiyai на основу тринаест секвенци гена cox1 (318bp) и осам секвенци 12S rDNA (238bp). H. kamiyai је регистрована код 17 A. flavicollis, једног A. agrarius, два M. arvalis и једне јединке C. leucodon. Према median-joining мрежи хаплотипова, 13 секвенци H. kamiyai cox1 из ове студије груписале су се са изолатима из Европе и западног Сибира који се разликују до 2.7%. Ови резултати потврђују и наглашавају различиту географску дистрибуцију и молекуларне разлике у роду Hydatigera. Резултати такође потврђују погодност испитиваних домаћина за ову врсту. Ово је први извештај ове ново-описане криптичне врсте код нас потврђен генетском анализом.
PB  - Belgrade: Veterinary Association of Serbia
C3  - Book of Abstracts: 25th Symposium of Epizootiologist and Epidemiologist; 2023 Apr 24-26; Novi Sad, Serbia
T1  - First report of recently described cryptic species Hydatigera kamiyai in Serbia
T1  - Први приказ недавно описане криптичне врсте Hydatigera kamiyai у србији
SP  - 74
EP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6033
ER  - 

@conference{
author = "Miljević, Milan and Rajičić, Marija and Umhang, Gerald and Bjelić Čabrilo, Olivera and Bajić, Branka and Budinski, Ivana and Blagojević, Jelena",
year = "2023",
abstract = "The family Taeniidae recently consists of 4 genera, with a newly described cryptic species H. kamiyai within the Hydatigera genus. H. kamiyai is distributed across Europe to western Siberia, whereas H. taeniaeformis s.s. probably originated in Asia but has spread worldwide. Despite the extraordinary genetic divergence, the two species differ little morphologically, making it questionable whether morphological identification is even relevant in this case. Considering the geographical location of the studied area and the intermediate hosts targeted, we expected the presence of H. kamiyai, and our aim was to confirm this assumption by genetic analyses. From March to October 2013 to 2022, a total of 846 small mammals (Apodemus agrarius (152), Apodemus flavicollis (521), Apodemus sylvaticus (33), Microtus arvalis (48), Microtus subteraneus (17), Myodes glareolus (52), Crocidura suaveolens (13), Crocidura leucodon (7) and Sorex araneus (3)) were captured from 45 different localities in Serbia. Cysts and visible lesions in the body cavity and various organs were examined. Two markers (cox1 and 12S rDNA) were used for amplification and sequencing. A total of 21 strobilocercus of H. kamiyai were identified corresponding to thirteen sequences of the cox1 gene (318 bp) and eight of 12S rDNA (238 bp). H. kamiyai is found in 17 A. flavicollis, one A. agrarius, two M. arvalis, and one C. leucodon. According to the median-joining network, the 13 H. kamiyai cox1 sequences from this study clustered with isolates from Europe and western Siberia differing up to 2.7%. These results confirm and highlight the different geographic distribution and molecular differences in the Hydatigera genus. The results also confirm host suitability for this species. This is the first report of this newly described cryptic species in our country confirmed by genetic analysis., Фамилија Taeniidae од недавно се састоји од 4 рода, са ново-описаном криптичном врстом Hydatigera kamiyai у оквиру рода Hydatigera. H. kamiyai врста је распрострањена широм Европе до западног Сибира, док H. taeniaeformis s.s вероватно потиче из Азије, али је распрострањена широм света. Упркос изузетној генетској дивергенцији, ове две врсте се мало разликују морфолошки, што доводи у питање да ли је морфолошка идентификација уопште релевантна у овом случају. С обзиром на географску локацију проучаваног подручја и циљане домаћине, очекивали смо присуство врсте H. kamiyai, са циљем да генетским анализама потврдимо ову претпоставку. Од марта до октобра 2013. до 2022., укупно 846 ситних сисара (Apodemus agrarius (152), Apodemus flavicollis (521), Apodemus sylvaticus (33), Microtus arvalis (48), Microtus subteraneus (17), Myodes glareolus (52), Crocidura suaveolens (13), Crocidura leucodon (7) и Sorex araneus (3)) прикупљено је са 45 различитих локалитета у Србији. Прегледане су цисте и видљиве лезије у телесној шупљини и разним органима. Два маркера (cox1 and 12S rDNA) коришћена су за амплификацију и секвенцирање. Идентификовано је укупно 21 strobilocercus H. kamiyai на основу тринаест секвенци гена cox1 (318bp) и осам секвенци 12S rDNA (238bp). H. kamiyai је регистрована код 17 A. flavicollis, једног A. agrarius, два M. arvalis и једне јединке C. leucodon. Према median-joining мрежи хаплотипова, 13 секвенци H. kamiyai cox1 из ове студије груписале су се са изолатима из Европе и западног Сибира који се разликују до 2.7%. Ови резултати потврђују и наглашавају различиту географску дистрибуцију и молекуларне разлике у роду Hydatigera. Резултати такође потврђују погодност испитиваних домаћина за ову врсту. Ово је први извештај ове ново-описане криптичне врсте код нас потврђен генетском анализом.",
publisher = "Belgrade: Veterinary Association of Serbia",
journal = "Book of Abstracts: 25th Symposium of Epizootiologist and Epidemiologist; 2023 Apr 24-26; Novi Sad, Serbia",
title = "First report of recently described cryptic species Hydatigera kamiyai in Serbia, Први приказ недавно описане криптичне врсте Hydatigera kamiyai у србији",
pages = "74-75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6033"
}

Miljević, M., Rajičić, M., Umhang, G., Bjelić Čabrilo, O., Bajić, B., Budinski, I.,& Blagojević, J.. (2023). First report of recently described cryptic species Hydatigera kamiyai in Serbia. in Book of Abstracts: 25th Symposium of Epizootiologist and Epidemiologist; 2023 Apr 24-26; Novi Sad, Serbia
Belgrade: Veterinary Association of Serbia., 74-75.
https://hdl.handle.net/21.15107/rcub_ibiss_6033

Miljević M, Rajičić M, Umhang G, Bjelić Čabrilo O, Bajić B, Budinski I, Blagojević J. First report of recently described cryptic species Hydatigera kamiyai in Serbia. in Book of Abstracts: 25th Symposium of Epizootiologist and Epidemiologist; 2023 Apr 24-26; Novi Sad, Serbia. 2023;:74-75.
https://hdl.handle.net/21.15107/rcub_ibiss_6033 .

Miljević, Milan, Rajičić, Marija, Umhang, Gerald, Bjelić Čabrilo, Olivera, Bajić, Branka, Budinski, Ivana, Blagojević, Jelena, "First report of recently described cryptic species Hydatigera kamiyai in Serbia" in Book of Abstracts: 25th Symposium of Epizootiologist and Epidemiologist; 2023 Apr 24-26; Novi Sad, Serbia (2023):74-75,
https://hdl.handle.net/21.15107/rcub_ibiss_6033 .