TY  - CONF
AU  - Vučetić, Milica
AU  - Markelić, Milica
AU  - Janković, Aleksandra
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Korać, Aleksandra
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1910
AB  - Natural hypothermia, in addition to allowing energy saving in hostile conditions, has been associated with delayed aging and increased longevity. However, the molecular basis responsible for observed correlations between the use of daily torpor/hibernation and indices of rate of aging is hitherto unclear. Considering central role of mitochondria dysfunction in the ageing process, we examined several mechanisms that might be involved in mitochondrial protection against oxidative damage during euthermia-hypothermia (and vice versa) transition, in brown adipose tissue (BAT) of the European Ground Squirrel (Spermophilus citellus).

Results showed that in hibernation increased protein expression of Mn superoxide dismutase coincides with decreased content of ATP synthase and uncoupling protein 1. This suggests that BAT mitochondria during hibernation are protected from oxidative injuries by suppressed oxidative capacity, as well as by upregulated antioxidant defense. Also, the data indicate that such molecular pattern of changes is initiated already during prehibernating period. Namely, in this period we observed accumulations of hypoxia-inducible factor-1α (HIF-1α) and nuclear factor (erythroid 2-related)-like 2, which are probably responsible for suppressed oxidative metabolism, i.e. increased antioxidant capacity, respectively. Increased expression of the mitofusin 1 and detection of the megamitochondria in the prehibernating period indicate intensive mitofusion process in the BAT. This may be another mechanism of protection of mitochondrial content/function during euthermia-hypothermia transition.

The results of the study suggest mechanisms that might be associated with increased resistance of “hibernating” mitochondria to the oxidative damage. Also, the data showed that biochemistry responsible for redox balance within the cell involves integration of antioxidant response and transcription control of overall metabolism. Finally, the results go in favor of the previous reports that suggested HIF-1 as a negative modulator of aging.
PB  - Elsevier
C3  - SFRR-E/SNFS Conference Abstracts; 2015 Sep 1-4; Stuttgart, Germany
T1  - Molecular mechanisms of mitochondrial protection against oxidative
 damage in hibernators - the anti-aging effects of heterothermy
IS  - 1
VL  - 86
DO  - 10.1016/j.freeradbiomed.2015.07.028
ER  - 

@conference{
author = "Vučetić, Milica and Markelić, Milica and Janković, Aleksandra and Stančić, Ana and Otašević, Vesna and Korać, Aleksandra and Buzadžić, Biljana J. and Korać, Bato",
year = "2015",
abstract = "Natural hypothermia, in addition to allowing energy saving in hostile conditions, has been associated with delayed aging and increased longevity. However, the molecular basis responsible for observed correlations between the use of daily torpor/hibernation and indices of rate of aging is hitherto unclear. Considering central role of mitochondria dysfunction in the ageing process, we examined several mechanisms that might be involved in mitochondrial protection against oxidative damage during euthermia-hypothermia (and vice versa) transition, in brown adipose tissue (BAT) of the European Ground Squirrel (Spermophilus citellus).

Results showed that in hibernation increased protein expression of Mn superoxide dismutase coincides with decreased content of ATP synthase and uncoupling protein 1. This suggests that BAT mitochondria during hibernation are protected from oxidative injuries by suppressed oxidative capacity, as well as by upregulated antioxidant defense. Also, the data indicate that such molecular pattern of changes is initiated already during prehibernating period. Namely, in this period we observed accumulations of hypoxia-inducible factor-1α (HIF-1α) and nuclear factor (erythroid 2-related)-like 2, which are probably responsible for suppressed oxidative metabolism, i.e. increased antioxidant capacity, respectively. Increased expression of the mitofusin 1 and detection of the megamitochondria in the prehibernating period indicate intensive mitofusion process in the BAT. This may be another mechanism of protection of mitochondrial content/function during euthermia-hypothermia transition.

The results of the study suggest mechanisms that might be associated with increased resistance of “hibernating” mitochondria to the oxidative damage. Also, the data showed that biochemistry responsible for redox balance within the cell involves integration of antioxidant response and transcription control of overall metabolism. Finally, the results go in favor of the previous reports that suggested HIF-1 as a negative modulator of aging.",
publisher = "Elsevier",
journal = "SFRR-E/SNFS Conference Abstracts; 2015 Sep 1-4; Stuttgart, Germany",
title = "Molecular mechanisms of mitochondrial protection against oxidative
 damage in hibernators - the anti-aging effects of heterothermy",
number = "1",
volume = "86",
doi = "10.1016/j.freeradbiomed.2015.07.028"
}

Vučetić, M., Markelić, M., Janković, A., Stančić, A., Otašević, V., Korać, A., Buzadžić, B. J.,& Korać, B.. (2015). Molecular mechanisms of mitochondrial protection against oxidative
 damage in hibernators - the anti-aging effects of heterothermy. in SFRR-E/SNFS Conference Abstracts; 2015 Sep 1-4; Stuttgart, Germany
Elsevier., 86(1).
https://doi.org/10.1016/j.freeradbiomed.2015.07.028

Vučetić M, Markelić M, Janković A, Stančić A, Otašević V, Korać A, Buzadžić BJ, Korać B. Molecular mechanisms of mitochondrial protection against oxidative
 damage in hibernators - the anti-aging effects of heterothermy. in SFRR-E/SNFS Conference Abstracts; 2015 Sep 1-4; Stuttgart, Germany. 2015;86(1).
doi:10.1016/j.freeradbiomed.2015.07.028 .

Vučetić, Milica, Markelić, Milica, Janković, Aleksandra, Stančić, Ana, Otašević, Vesna, Korać, Aleksandra, Buzadžić, Biljana J., Korać, Bato, "Molecular mechanisms of mitochondrial protection against oxidative
 damage in hibernators - the anti-aging effects of heterothermy" in SFRR-E/SNFS Conference Abstracts; 2015 Sep 1-4; Stuttgart, Germany, 86, no. 1 (2015),
https://doi.org/10.1016/j.freeradbiomed.2015.07.028 . .

TY  - JOUR
AU  - Buzadžić, Biljana J.
AU  - Vucetic, M.
AU  - Janković, Aleksandra
AU  - Stancic, A.
AU  - Korac, A.
AU  - Korać, Bato
AU  - Otašević, Vesna
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1994
AB  - Infertility is a global problem that is on the rise, especially during
   the last decade. Currently, infertility affects approximately 10-15\% of
   the population worldwide. The frequency and origin of different forms of
   infertility varies. It has been shown that reactive oxygen and nitrogen
   species (ROS and RNS) are involved in the aetiology of infertility,
   especially male infertility. Various strategies have been designed to
   remove or decrease the production of ROS and RNS in spermatozoa, in
   particular during in vitro fertilization. However, in recent years it
   has been shown that spermatozoa naturally produce a variety of ROS/RNS,
   including superoxide anion radical (O-2(-)), hydrogen peroxide and NO.
   These reactive species, in particular NO, are essential in regulating
   sperm capacitation and the acrosome reaction, two processes that need to
   be acquired by sperm in order to achieve fertilization potential. In
   addition, it has recently been shown that mitochondrial function is
   positively correlated with human sperm fertilization potential and
   quality and that NO and NO precursors increase sperm motility by
   increasing energy production in mitochondria. We will review the new
   link between sperm NO-driven redox regulation and infertility herein. A
   special emphasis will be placed on the potential implementation of new
   redox-active substances that modulate the content of NO in spermatozoa
   to increase fertility and promote conception.
   Linked ArticlesThis article is part of a themed section on Pharmacology
   of the Gasotransmitters. To view the other articles in this section
   visit
PB  - John Wiley and Sons
T2  - British Journal of Pharmacology
T1  - New insights into male (in)fertility: the importance of NO
IS  - 6, SI
VL  - 172
DO  - 10.1111/bph.12675
SP  - 1455
EP  - 1467
ER  - 

@article{
author = "Buzadžić, Biljana J. and Vucetic, M. and Janković, Aleksandra and Stancic, A. and Korac, A. and Korać, Bato and Otašević, Vesna",
year = "2015",
abstract = "Infertility is a global problem that is on the rise, especially during
   the last decade. Currently, infertility affects approximately 10-15\% of
   the population worldwide. The frequency and origin of different forms of
   infertility varies. It has been shown that reactive oxygen and nitrogen
   species (ROS and RNS) are involved in the aetiology of infertility,
   especially male infertility. Various strategies have been designed to
   remove or decrease the production of ROS and RNS in spermatozoa, in
   particular during in vitro fertilization. However, in recent years it
   has been shown that spermatozoa naturally produce a variety of ROS/RNS,
   including superoxide anion radical (O-2(-)), hydrogen peroxide and NO.
   These reactive species, in particular NO, are essential in regulating
   sperm capacitation and the acrosome reaction, two processes that need to
   be acquired by sperm in order to achieve fertilization potential. In
   addition, it has recently been shown that mitochondrial function is
   positively correlated with human sperm fertilization potential and
   quality and that NO and NO precursors increase sperm motility by
   increasing energy production in mitochondria. We will review the new
   link between sperm NO-driven redox regulation and infertility herein. A
   special emphasis will be placed on the potential implementation of new
   redox-active substances that modulate the content of NO in spermatozoa
   to increase fertility and promote conception.
   Linked ArticlesThis article is part of a themed section on Pharmacology
   of the Gasotransmitters. To view the other articles in this section
   visit",
publisher = "John Wiley and Sons",
journal = "British Journal of Pharmacology",
title = "New insights into male (in)fertility: the importance of NO",
number = "6, SI",
volume = "172",
doi = "10.1111/bph.12675",
pages = "1455-1467"
}

Buzadžić, B. J., Vucetic, M., Janković, A., Stancic, A., Korac, A., Korać, B.,& Otašević, V.. (2015). New insights into male (in)fertility: the importance of NO. in British Journal of Pharmacology
John Wiley and Sons., 172(6, SI), 1455-1467.
https://doi.org/10.1111/bph.12675

Buzadžić BJ, Vucetic M, Janković A, Stancic A, Korac A, Korać B, Otašević V. New insights into male (in)fertility: the importance of NO. in British Journal of Pharmacology. 2015;172(6, SI):1455-1467.
doi:10.1111/bph.12675 .

Buzadžić, Biljana J., Vucetic, M., Janković, Aleksandra, Stancic, A., Korac, A., Korać, Bato, Otašević, Vesna, "New insights into male (in)fertility: the importance of NO" in British Journal of Pharmacology, 172, no. 6, SI (2015):1455-1467,
https://doi.org/10.1111/bph.12675 . .

TY  - JOUR
AU  - Janković, Aleksandra
AU  - Korac, Aleksandra
AU  - Buzadžić, Biljana J.
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Daiber, Andreas
AU  - Korać, Bato
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2326
AB  - Obesity is an energy balance disorder associated with dyslipidemia,
   insulin resistance and diabetes type 2, also summarized with the term
   metabolic syndrome or syndrome X. Increasing evidence points to
   ``adipocyte dysfunction{''}, rather than fat mass accretion per se, as
   the key pathophysiological factor for metabolic complications in
   obesity. The dysfunctional fat tissue in obesity characterizes a failure
   to safely store metabolic substrates into existing hypertrophied
   adipocytes and/or into new preadipocytes recruited for differentiation.
   In this review we briefly summarize the potential of redox imbalance in
   fat tissue as an instigator of adipocyte dysfunction in obesity. We
   reveal the challenge of the adipose redox changes, insights in the
   regulation of healthy expansion of adipose tissue and its reduction,
   leading to glucose and lipids overflow. (C) 2015 Published by Elsevier
   B.V.
T2  - Redox Biology
T1  - Redox implications in adipose tissue (dys)function-A new look at old
 acquaintances
VL  - 6
DO  - 10.1016/j.redox.2015.06.018
SP  - 19
EP  - 32
ER  - 

@article{
author = "Janković, Aleksandra and Korac, Aleksandra and Buzadžić, Biljana J. and Otašević, Vesna and Stančić, Ana and Daiber, Andreas and Korać, Bato",
year = "2015",
abstract = "Obesity is an energy balance disorder associated with dyslipidemia,
   insulin resistance and diabetes type 2, also summarized with the term
   metabolic syndrome or syndrome X. Increasing evidence points to
   ``adipocyte dysfunction{''}, rather than fat mass accretion per se, as
   the key pathophysiological factor for metabolic complications in
   obesity. The dysfunctional fat tissue in obesity characterizes a failure
   to safely store metabolic substrates into existing hypertrophied
   adipocytes and/or into new preadipocytes recruited for differentiation.
   In this review we briefly summarize the potential of redox imbalance in
   fat tissue as an instigator of adipocyte dysfunction in obesity. We
   reveal the challenge of the adipose redox changes, insights in the
   regulation of healthy expansion of adipose tissue and its reduction,
   leading to glucose and lipids overflow. (C) 2015 Published by Elsevier
   B.V.",
journal = "Redox Biology",
title = "Redox implications in adipose tissue (dys)function-A new look at old
 acquaintances",
volume = "6",
doi = "10.1016/j.redox.2015.06.018",
pages = "19-32"
}

Janković, A., Korac, A., Buzadžić, B. J., Otašević, V., Stančić, A., Daiber, A.,& Korać, B.. (2015). Redox implications in adipose tissue (dys)function-A new look at old
 acquaintances. in Redox Biology, 6, 19-32.
https://doi.org/10.1016/j.redox.2015.06.018

Janković A, Korac A, Buzadžić BJ, Otašević V, Stančić A, Daiber A, Korać B. Redox implications in adipose tissue (dys)function-A new look at old
 acquaintances. in Redox Biology. 2015;6:19-32.
doi:10.1016/j.redox.2015.06.018 .

Janković, Aleksandra, Korac, Aleksandra, Buzadžić, Biljana J., Otašević, Vesna, Stančić, Ana, Daiber, Andreas, Korać, Bato, "Redox implications in adipose tissue (dys)function-A new look at old
 acquaintances" in Redox Biology, 6 (2015):19-32,
https://doi.org/10.1016/j.redox.2015.06.018 . .

TY  - JOUR
AU  - Janković, Aleksandra
AU  - Golic, Igor
AU  - Markelic, Milica
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra
AU  - Korać, Bato
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1921
AB  - Conversion of white into brown adipose tissue may have important
   implications in obesity resistance and treatment. Several browning
   agents or conditions ignite thermogenesis in white adipose tissue (WAT).
   To reveal the capacity of WAT to function in a brownish/burning mode
   over the long term, we investigated the progression of the rat
   retroperitoneal WAT (rpWAT) browning during 45days of cold acclimation.
   During the early stages of cold acclimation, the majority of rpWAT
   adipocytes underwent multilocularization and thermogenic-profile
   induction, as demonstrated by the presence of a multitude of uncoupling
   protein 1 (UCP1)-immunopositive paucilocular adipocytes containing
   peroxisome proliferator-activated receptor (PPAR) coactivator-1 (PGC-1)
   and PR domain-containing 16 (PRDM16) in their nuclei. After 45days, all
   adipocytes remained PRDM16 immunopositive, but only a few multilocular
   adipocytes rich in mitochondria remained UCP1/PGC-1 immunopositive.
   Molecular evidence showed that thermogenic recruitment of rpWAT occurred
   following cold exposure, but returned to starting levels after cold
   acclimation. Compared with controls (22 +/- 1 degrees C), levels of UCP1
   mRNA increased in parallel with PPAR (PPAR from days 1 to 7 and PGC-1 on
   day 1). Transcriptional recruitment of rpWAT was followed by an increase
   in UCP1 protein content (from days 1 to 21). Results clearly showed that
   most of the adipocytes within rpWAT underwent transient brown-fat-like
   thermogenic recruitment upon stimulation, but only a minority of cells
   retained a brown adipose tissue-like phenotype after the attainment of
   cold acclimation. Therefore, browning of WAT is dependent on both
   maintaining the thermogenic response and retaining enough brown-like
   thermogenically competent adipocytes in the long-term. Both aspects of
   browning could be important for long-term energy homeostasis and
   body-weight regulation.
T2  - Journal of Physiology-London
T1  - Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation
IS  - 15
VL  - 593
DO  - 10.1113/JP270805
SP  - 3267
EP  - 3280
ER  - 

@article{
author = "Janković, Aleksandra and Golic, Igor and Markelic, Milica and Stančić, Ana and Otašević, Vesna and Buzadžić, Biljana J. and Korac, Aleksandra and Korać, Bato",
year = "2015",
abstract = "Conversion of white into brown adipose tissue may have important
   implications in obesity resistance and treatment. Several browning
   agents or conditions ignite thermogenesis in white adipose tissue (WAT).
   To reveal the capacity of WAT to function in a brownish/burning mode
   over the long term, we investigated the progression of the rat
   retroperitoneal WAT (rpWAT) browning during 45days of cold acclimation.
   During the early stages of cold acclimation, the majority of rpWAT
   adipocytes underwent multilocularization and thermogenic-profile
   induction, as demonstrated by the presence of a multitude of uncoupling
   protein 1 (UCP1)-immunopositive paucilocular adipocytes containing
   peroxisome proliferator-activated receptor (PPAR) coactivator-1 (PGC-1)
   and PR domain-containing 16 (PRDM16) in their nuclei. After 45days, all
   adipocytes remained PRDM16 immunopositive, but only a few multilocular
   adipocytes rich in mitochondria remained UCP1/PGC-1 immunopositive.
   Molecular evidence showed that thermogenic recruitment of rpWAT occurred
   following cold exposure, but returned to starting levels after cold
   acclimation. Compared with controls (22 +/- 1 degrees C), levels of UCP1
   mRNA increased in parallel with PPAR (PPAR from days 1 to 7 and PGC-1 on
   day 1). Transcriptional recruitment of rpWAT was followed by an increase
   in UCP1 protein content (from days 1 to 21). Results clearly showed that
   most of the adipocytes within rpWAT underwent transient brown-fat-like
   thermogenic recruitment upon stimulation, but only a minority of cells
   retained a brown adipose tissue-like phenotype after the attainment of
   cold acclimation. Therefore, browning of WAT is dependent on both
   maintaining the thermogenic response and retaining enough brown-like
   thermogenically competent adipocytes in the long-term. Both aspects of
   browning could be important for long-term energy homeostasis and
   body-weight regulation.",
journal = "Journal of Physiology-London",
title = "Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation",
number = "15",
volume = "593",
doi = "10.1113/JP270805",
pages = "3267-3280"
}

Janković, A., Golic, I., Markelic, M., Stančić, A., Otašević, V., Buzadžić, B. J., Korac, A.,& Korać, B.. (2015). Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation. in Journal of Physiology-London, 593(15), 3267-3280.
https://doi.org/10.1113/JP270805

Janković A, Golic I, Markelic M, Stančić A, Otašević V, Buzadžić BJ, Korac A, Korać B. Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation. in Journal of Physiology-London. 2015;593(15):3267-3280.
doi:10.1113/JP270805 .

Janković, Aleksandra, Golic, Igor, Markelic, Milica, Stančić, Ana, Otašević, Vesna, Buzadžić, Biljana J., Korac, Aleksandra, Korać, Bato, "Two key temporally distinguishable molecular and cellular components of
 white adipose tissue browning during cold acclimation" in Journal of Physiology-London, 593, no. 15 (2015):3267-3280,
https://doi.org/10.1113/JP270805 . .

TY  - JOUR
AU  - Macanović, Biljana
AU  - Vučetić, Milica
AU  - Janković, Aleksandra
AU  - Stančić, Ana
AU  - Buzadžić, Biljana J.
AU  - Garalejić, Eliana
AU  - Korać, Aleksandra
AU  - Korać, Bato
AU  - Otašević, Vesna
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2090
AB  - Background. Semen analysis is the cornerstone in the evaluation of male
   (in) fertility. However, there are men with normal semen tests but with
   impaired fertilizing ability, as well as fertile men with poor sperm
   characteristics. Thus, there is rising interest to find novel parameters
   that will help to predict and define the functional capacity of
   spermatozoa. Methods. We examined whether there is a correlation between
   semen parameters (count, progressive motility, and morphology) and
   protein expression/activity of antioxidative defense enzymes in seminal
   plasma from 10 normospermic subjects. Results. Sperm progressive
   motility was in positive correlation with seminal plasma protein
   expression of both superoxide dismutase (SOD) isoforms (MnSOD and
   CuZnSOD) and catalase. Also, positive correlation was observed between
   sperm count and MnSOD protein expression, as well as between sperm
   morphology and protein expression of catalase in seminal plasma. In
   contrast, protein expression of glutathione peroxidase was not in
   correlation with any sperm parameter, while its activity negatively
   correlated with sperm morphology and motility. Conclusions. These data
   suggest that evaluation of protein expression of antioxidative defense
   enzymes in seminal plasma might be of importance in the evaluation of
   male fertility status and that could be used as an additional biomarker
   along with classic semen analysis in assessment of semen quality.
T2  - Disease Markers
T1  - Correlation between Sperm Parameters and Protein Expression of
 Antioxidative Defense Enzymes in Seminal Plasma: A Pilot Study
IS  - 436236
DO  - 10.1155/2015/436236
ER  - 

@article{
author = "Macanović, Biljana and Vučetić, Milica and Janković, Aleksandra and Stančić, Ana and Buzadžić, Biljana J. and Garalejić, Eliana and Korać, Aleksandra and Korać, Bato and Otašević, Vesna",
year = "2015",
abstract = "Background. Semen analysis is the cornerstone in the evaluation of male
   (in) fertility. However, there are men with normal semen tests but with
   impaired fertilizing ability, as well as fertile men with poor sperm
   characteristics. Thus, there is rising interest to find novel parameters
   that will help to predict and define the functional capacity of
   spermatozoa. Methods. We examined whether there is a correlation between
   semen parameters (count, progressive motility, and morphology) and
   protein expression/activity of antioxidative defense enzymes in seminal
   plasma from 10 normospermic subjects. Results. Sperm progressive
   motility was in positive correlation with seminal plasma protein
   expression of both superoxide dismutase (SOD) isoforms (MnSOD and
   CuZnSOD) and catalase. Also, positive correlation was observed between
   sperm count and MnSOD protein expression, as well as between sperm
   morphology and protein expression of catalase in seminal plasma. In
   contrast, protein expression of glutathione peroxidase was not in
   correlation with any sperm parameter, while its activity negatively
   correlated with sperm morphology and motility. Conclusions. These data
   suggest that evaluation of protein expression of antioxidative defense
   enzymes in seminal plasma might be of importance in the evaluation of
   male fertility status and that could be used as an additional biomarker
   along with classic semen analysis in assessment of semen quality.",
journal = "Disease Markers",
title = "Correlation between Sperm Parameters and Protein Expression of
 Antioxidative Defense Enzymes in Seminal Plasma: A Pilot Study",
number = "436236",
doi = "10.1155/2015/436236"
}

Macanović, B., Vučetić, M., Janković, A., Stančić, A., Buzadžić, B. J., Garalejić, E., Korać, A., Korać, B.,& Otašević, V.. (2015). Correlation between Sperm Parameters and Protein Expression of
 Antioxidative Defense Enzymes in Seminal Plasma: A Pilot Study. in Disease Markers(436236).
https://doi.org/10.1155/2015/436236

Macanović B, Vučetić M, Janković A, Stančić A, Buzadžić BJ, Garalejić E, Korać A, Korać B, Otašević V. Correlation between Sperm Parameters and Protein Expression of
 Antioxidative Defense Enzymes in Seminal Plasma: A Pilot Study. in Disease Markers. 2015;(436236).
doi:10.1155/2015/436236 .

Macanović, Biljana, Vučetić, Milica, Janković, Aleksandra, Stančić, Ana, Buzadžić, Biljana J., Garalejić, Eliana, Korać, Aleksandra, Korać, Bato, Otašević, Vesna, "Correlation between Sperm Parameters and Protein Expression of
 Antioxidative Defense Enzymes in Seminal Plasma: A Pilot Study" in Disease Markers, no. 436236 (2015),
https://doi.org/10.1155/2015/436236 . .

TY  - JOUR
AU  - Veličković, Ksenija
AU  - Čvoro, Aleksandra
AU  - Srdić, Biljana
AU  - Stokić, Edita
AU  - Markelić, Milica
AU  - Golić, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2294
AB  - Context: Brown adipose tissue (BAT) has the unique ability of generating
   heat due to the expression of mitochondrial uncoupling protein 1 (UCP1).
   A recent discovery regarding functional BAT in adult humans has
   increased interest in the molecular pathways of BAT development and
   functionality. An important role for estrogen in white adipose tissue
   was shown, but the possible role of estrogen in human fetal BAT (fBAT)
   is unclear.
   Objective: The objective of this study was to determine whether human
   fBAT expresses estrogen receptor alpha (ER alpha) and ER beta. In
   addition, we examined their localization as well as their correlation
   with crucial proteins involved in BAT differentiation, proliferation,
   mitochondriogenesis and thermogenesis including peroxisome
   proliferator-activated receptor gamma (PPAR gamma), proliferating cell
   nuclear antigen (PCNA), PPAR gamma-coactivator-1 alpha (PGC-1 alpha),
   and UCP1.
   Design: The fBAT was obtained from 4 human male fetuses aged 15, 17, 20,
   and 23 weeks gestation. ER alpha and ER beta expression was assessed
   using Western blotting, immunohistochemistry, and immunocytochemistry.
   Possible correlations with PPAR gamma, PCNA, PGC-1 alpha, and UCP1 were
   examined by double immunofluorescence.
   Results: Both ER alpha and ER beta were expressed in human fBAT, with ER
   alpha being dominant. Unlike ER beta, which was present only in mature
   brown adipocytes, we detected ER alpha in mature adipocytes,
   preadipocytes, mesenchymal and endothelial cells. In addition, double
   immunofluorescence supported the notion that differentiation in fBAT
   probably involves ER alpha. Immunocytochemical analysis revealed
   mitochondrial localization of both receptors.
   Conclusion: The expression of both ER alpha and ER beta in human fBAT
   suggests a role for estrogen in its development, primarily via ER alpha.
   In addition, our results indicate that fBAT mitochondria could be
   targeted by estrogens and pointed out the possible role of both ERs in
   mitochondriogenesis.
T2  - Journal of Clinical Endocrinology & Metabolism
T1  - Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue
IS  - 1
VL  - 99
DO  - 10.1210/jc.2013-2017
SP  - 151
EP  - 159
ER  - 

@article{
author = "Veličković, Ksenija and Čvoro, Aleksandra and Srdić, Biljana and Stokić, Edita and Markelić, Milica and Golić, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Buzadžić, Biljana J. and Korać, Bato and Korać, Aleksandra",
year = "2014",
abstract = "Context: Brown adipose tissue (BAT) has the unique ability of generating
   heat due to the expression of mitochondrial uncoupling protein 1 (UCP1).
   A recent discovery regarding functional BAT in adult humans has
   increased interest in the molecular pathways of BAT development and
   functionality. An important role for estrogen in white adipose tissue
   was shown, but the possible role of estrogen in human fetal BAT (fBAT)
   is unclear.
   Objective: The objective of this study was to determine whether human
   fBAT expresses estrogen receptor alpha (ER alpha) and ER beta. In
   addition, we examined their localization as well as their correlation
   with crucial proteins involved in BAT differentiation, proliferation,
   mitochondriogenesis and thermogenesis including peroxisome
   proliferator-activated receptor gamma (PPAR gamma), proliferating cell
   nuclear antigen (PCNA), PPAR gamma-coactivator-1 alpha (PGC-1 alpha),
   and UCP1.
   Design: The fBAT was obtained from 4 human male fetuses aged 15, 17, 20,
   and 23 weeks gestation. ER alpha and ER beta expression was assessed
   using Western blotting, immunohistochemistry, and immunocytochemistry.
   Possible correlations with PPAR gamma, PCNA, PGC-1 alpha, and UCP1 were
   examined by double immunofluorescence.
   Results: Both ER alpha and ER beta were expressed in human fBAT, with ER
   alpha being dominant. Unlike ER beta, which was present only in mature
   brown adipocytes, we detected ER alpha in mature adipocytes,
   preadipocytes, mesenchymal and endothelial cells. In addition, double
   immunofluorescence supported the notion that differentiation in fBAT
   probably involves ER alpha. Immunocytochemical analysis revealed
   mitochondrial localization of both receptors.
   Conclusion: The expression of both ER alpha and ER beta in human fBAT
   suggests a role for estrogen in its development, primarily via ER alpha.
   In addition, our results indicate that fBAT mitochondria could be
   targeted by estrogens and pointed out the possible role of both ERs in
   mitochondriogenesis.",
journal = "Journal of Clinical Endocrinology & Metabolism",
title = "Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue",
number = "1",
volume = "99",
doi = "10.1210/jc.2013-2017",
pages = "151-159"
}

Veličković, K., Čvoro, A., Srdić, B., Stokić, E., Markelić, M., Golić, I., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Buzadžić, B. J., Korać, B.,& Korać, A.. (2014). Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue. in Journal of Clinical Endocrinology & Metabolism, 99(1), 151-159.
https://doi.org/10.1210/jc.2013-2017

Veličković K, Čvoro A, Srdić B, Stokić E, Markelić M, Golić I, Otašević V, Stančić A, Janković A, Vučetić M, Buzadžić BJ, Korać B, Korać A. Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue. in Journal of Clinical Endocrinology & Metabolism. 2014;99(1):151-159.
doi:10.1210/jc.2013-2017 .

Veličković, Ksenija, Čvoro, Aleksandra, Srdić, Biljana, Stokić, Edita, Markelić, Milica, Golić, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Buzadžić, Biljana J., Korać, Bato, Korać, Aleksandra, "Expression and Subcellular Localization of Estrogen Receptors alpha and
 beta in Human Fetal Brown Adipose Tissue" in Journal of Clinical Endocrinology & Metabolism, 99, no. 1 (2014):151-159,
https://doi.org/10.1210/jc.2013-2017 . .

TY  - CONF
AU  - Vujic, D. Stojan
AU  - Lazic, E.
AU  - Stancic, A.
AU  - Otašević, Vesna
AU  - Vucetic, M.
AU  - Jankovic, A.
AU  - Buzadžić, Biljana J.
AU  - Zecevic, Z.
AU  - Korac, A.
AU  - Korać, Bato
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2239
C3  - Bone Marrow Transplantation
T1  - METABOLIC CHANGES IN PERIPHERAL BLOOD STEM CELLS OF PEDIATRIC PATIENTS
 FOR AUTOLOGOUS TRANSPLANTATION WITH DIFFERENT TYPE OF CANCER
IS  - 1
VL  - 49
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2239
ER  - 

@conference{
author = "Vujic, D. Stojan and Lazic, E. and Stancic, A. and Otašević, Vesna and Vucetic, M. and Jankovic, A. and Buzadžić, Biljana J. and Zecevic, Z. and Korac, A. and Korać, Bato",
year = "2014",
journal = "Bone Marrow Transplantation",
title = "METABOLIC CHANGES IN PERIPHERAL BLOOD STEM CELLS OF PEDIATRIC PATIENTS
 FOR AUTOLOGOUS TRANSPLANTATION WITH DIFFERENT TYPE OF CANCER",
number = "1",
volume = "49",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2239"
}

Vujic, D. S., Lazic, E., Stancic, A., Otašević, V., Vucetic, M., Jankovic, A., Buzadžić, B. J., Zecevic, Z., Korac, A.,& Korać, B.. (2014). METABOLIC CHANGES IN PERIPHERAL BLOOD STEM CELLS OF PEDIATRIC PATIENTS
 FOR AUTOLOGOUS TRANSPLANTATION WITH DIFFERENT TYPE OF CANCER. in Bone Marrow Transplantation, 49(1).
https://hdl.handle.net/21.15107/rcub_ibiss_2239

Vujic DS, Lazic E, Stancic A, Otašević V, Vucetic M, Jankovic A, Buzadžić BJ, Zecevic Z, Korac A, Korać B. METABOLIC CHANGES IN PERIPHERAL BLOOD STEM CELLS OF PEDIATRIC PATIENTS
 FOR AUTOLOGOUS TRANSPLANTATION WITH DIFFERENT TYPE OF CANCER. in Bone Marrow Transplantation. 2014;49(1).
https://hdl.handle.net/21.15107/rcub_ibiss_2239 .

Vujic, D. Stojan, Lazic, E., Stancic, A., Otašević, Vesna, Vucetic, M., Jankovic, A., Buzadžić, Biljana J., Zecevic, Z., Korac, A., Korać, Bato, "METABOLIC CHANGES IN PERIPHERAL BLOOD STEM CELLS OF PEDIATRIC PATIENTS
 FOR AUTOLOGOUS TRANSPLANTATION WITH DIFFERENT TYPE OF CANCER" in Bone Marrow Transplantation, 49, no. 1 (2014),
https://hdl.handle.net/21.15107/rcub_ibiss_2239 .

TY  - JOUR
AU  - Golic, I.
AU  - Velickovic, K.
AU  - Markelic, M.
AU  - Stancic, A.
AU  - Jankovic, A.
AU  - Vucetic, M.
AU  - Otašević, Vesna
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korac, A.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2256
AB  - Mitochondria are key organelles maintaining cellular bioenergetics and
   integrity, and their regulation of {[}Ca2+](i) homeostasis has been
   investigated in many cell types. We investigated the short-term
   Ca-SANDOZ (R) treatment on brown adipocyte mitochondria, using imaging
   and molecular biology techniques. Two-month-old male Wistar rats were
   divided into two groups: Ca-SANDOZ (R) drinking or tap water (control)
   drinking for three days. Alizarin Red S staining showed increased Ca2+
   level in the brown adipocytes of treated rats, and potassium
   pyroantimonate staining localized electron-dense regions in the
   cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ (R)
   decreased mitochondrial number, but increased their size and
   mitochondrial cristae volume. Transmission electron microscopy revealed
   numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ (R)
   treated group compared to the control, and megamitochondria in some
   brown adipocytes. The Ca2+ diet affected mitochondrial fusion as
   mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and
   mitochondrial fission as dynamin related protein 1 (DRP1) was decreased.
   Confocal microscopy showed a higher colocalization rate between
   functional mitochondria and endoplasmic reticulum (ER). The level of
   uncoupling protein-1 (UCP1) was elevated, which was confirmed by
   immunohistochemistry and Western blot analysis. These results suggest
   that Ca-SANDOZ (R) stimulates mitochondrial fusion, increases
   mitochondrial-ER contacts and the thermogenic capacity of brown
   adipocytes.
T2  - European Journal of Histochemistry
T1  - Calcium-induced alteration of mitochondrial morphology and
 mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes
IS  - 2377
VL  - 58
DO  - 10.4081/ejh.2014.2377
SP  - 251
EP  - 257
ER  - 

@article{
author = "Golic, I. and Velickovic, K. and Markelic, M. and Stancic, A. and Jankovic, A. and Vucetic, M. and Otašević, Vesna and Buzadžić, Biljana J. and Korać, Bato and Korac, A.",
year = "2014",
abstract = "Mitochondria are key organelles maintaining cellular bioenergetics and
   integrity, and their regulation of {[}Ca2+](i) homeostasis has been
   investigated in many cell types. We investigated the short-term
   Ca-SANDOZ (R) treatment on brown adipocyte mitochondria, using imaging
   and molecular biology techniques. Two-month-old male Wistar rats were
   divided into two groups: Ca-SANDOZ (R) drinking or tap water (control)
   drinking for three days. Alizarin Red S staining showed increased Ca2+
   level in the brown adipocytes of treated rats, and potassium
   pyroantimonate staining localized electron-dense regions in the
   cytoplasm, mitochondria and around lipid droplets. Ca-SANDOZ (R)
   decreased mitochondrial number, but increased their size and
   mitochondrial cristae volume. Transmission electron microscopy revealed
   numerous enlarged and fusioned-like mitochondria in the Ca-SANDOZ (R)
   treated group compared to the control, and megamitochondria in some
   brown adipocytes. The Ca2+ diet affected mitochondrial fusion as
   mitofusin 1 (MFN1) and mitofusin 2 (MFN2) were increased, and
   mitochondrial fission as dynamin related protein 1 (DRP1) was decreased.
   Confocal microscopy showed a higher colocalization rate between
   functional mitochondria and endoplasmic reticulum (ER). The level of
   uncoupling protein-1 (UCP1) was elevated, which was confirmed by
   immunohistochemistry and Western blot analysis. These results suggest
   that Ca-SANDOZ (R) stimulates mitochondrial fusion, increases
   mitochondrial-ER contacts and the thermogenic capacity of brown
   adipocytes.",
journal = "European Journal of Histochemistry",
title = "Calcium-induced alteration of mitochondrial morphology and
 mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes",
number = "2377",
volume = "58",
doi = "10.4081/ejh.2014.2377",
pages = "251-257"
}

Golic, I., Velickovic, K., Markelic, M., Stancic, A., Jankovic, A., Vucetic, M., Otašević, V., Buzadžić, B. J., Korać, B.,& Korac, A.. (2014). Calcium-induced alteration of mitochondrial morphology and
 mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes. in European Journal of Histochemistry, 58(2377), 251-257.
https://doi.org/10.4081/ejh.2014.2377

Golic I, Velickovic K, Markelic M, Stancic A, Jankovic A, Vucetic M, Otašević V, Buzadžić BJ, Korać B, Korac A. Calcium-induced alteration of mitochondrial morphology and
 mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes. in European Journal of Histochemistry. 2014;58(2377):251-257.
doi:10.4081/ejh.2014.2377 .

Golic, I., Velickovic, K., Markelic, M., Stancic, A., Jankovic, A., Vucetic, M., Otašević, Vesna, Buzadžić, Biljana J., Korać, Bato, Korac, A., "Calcium-induced alteration of mitochondrial morphology and
 mitochondrial-endoplasmic reticulum contacts in rat brown adipocytes" in European Journal of Histochemistry, 58, no. 2377 (2014):251-257,
https://doi.org/10.4081/ejh.2014.2377 . .

TY  - JOUR
AU  - Velickovic, Ksenija
AU  - Markelic, Milica
AU  - Golic, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vucetic, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korac, Aleksandra
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2227
AB  - Nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) are
   important intestinal neurotransmitters that coexist in the gut enteric
   nervous system and play an important role in intestinal physiology
   (e.g., absorption, motility, fluid secretion and smooth muscle
   relaxation). It is also known that cold exposure alters several aspects
   of gastrointestinal physiology and induces hyperphagia to meet increased
   metabolic demands, but there are no data regarding NO and VIP
   involvement in intestinal response during acclimation to cold. The
   objective of this study was to determine the influence of long-term
   l-arginine supplementation on the expression of the three isoforms of
   nitric oxide synthase (NOS) and VIP in small intestine of rats
   acclimated to room temperature or cold.
   Animals (six per group) acclimated to room temperature (22 +/- A 1 A
   degrees C) and cold (4 +/- A 1 A degrees C), respectively, were treated
   with 2.25 \% l-arginine, a substrate for NOSs, or with 0.01 \% N
   (omega)-nitro-l-arginine methyl ester, an inhibitor of NOSs, for 45
   days. The topographical distribution of VIP and NOSs expression in small
   intestine was studied by immunohistochemistry, and ImageJ software was
   used for semiquantitative densitometric analysis of their
   immunoexpression.
   Long-term dietary l-arginine supplementation increases VIP and NOSs
   immunoexpression at room temperature while at cold increases the
   endothelial NOS, inducible NOS and VIP but decrease neuronal NOS in rat
   small intestine.
   Our results demonstrate that long-term dietary l-arginine
   supplementation modulates NOSs and VIP immunoexpression in rat small
   intestine with respect to ambient temperature, pointing out the eNOS as
   a predominant NOS isoform with an immunoexpression pattern similar to
   VIP.
T2  - European Journal of Nutrition
T1  - Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine
IS  - 3
VL  - 53
DO  - 10.1007/s00394-013-0585-8
SP  - 813
EP  - 821
ER  - 

@article{
author = "Velickovic, Ksenija and Markelic, Milica and Golic, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vucetic, Milica and Buzadžić, Biljana J. and Korać, Bato and Korac, Aleksandra",
year = "2014",
abstract = "Nitric oxide (NO) and vasoactive intestinal polypeptide (VIP) are
   important intestinal neurotransmitters that coexist in the gut enteric
   nervous system and play an important role in intestinal physiology
   (e.g., absorption, motility, fluid secretion and smooth muscle
   relaxation). It is also known that cold exposure alters several aspects
   of gastrointestinal physiology and induces hyperphagia to meet increased
   metabolic demands, but there are no data regarding NO and VIP
   involvement in intestinal response during acclimation to cold. The
   objective of this study was to determine the influence of long-term
   l-arginine supplementation on the expression of the three isoforms of
   nitric oxide synthase (NOS) and VIP in small intestine of rats
   acclimated to room temperature or cold.
   Animals (six per group) acclimated to room temperature (22 +/- A 1 A
   degrees C) and cold (4 +/- A 1 A degrees C), respectively, were treated
   with 2.25 \% l-arginine, a substrate for NOSs, or with 0.01 \% N
   (omega)-nitro-l-arginine methyl ester, an inhibitor of NOSs, for 45
   days. The topographical distribution of VIP and NOSs expression in small
   intestine was studied by immunohistochemistry, and ImageJ software was
   used for semiquantitative densitometric analysis of their
   immunoexpression.
   Long-term dietary l-arginine supplementation increases VIP and NOSs
   immunoexpression at room temperature while at cold increases the
   endothelial NOS, inducible NOS and VIP but decrease neuronal NOS in rat
   small intestine.
   Our results demonstrate that long-term dietary l-arginine
   supplementation modulates NOSs and VIP immunoexpression in rat small
   intestine with respect to ambient temperature, pointing out the eNOS as
   a predominant NOS isoform with an immunoexpression pattern similar to
   VIP.",
journal = "European Journal of Nutrition",
title = "Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine",
number = "3",
volume = "53",
doi = "10.1007/s00394-013-0585-8",
pages = "813-821"
}

Velickovic, K., Markelic, M., Golic, I., Otašević, V., Stančić, A., Janković, A., Vucetic, M., Buzadžić, B. J., Korać, B.,& Korac, A.. (2014). Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine. in European Journal of Nutrition, 53(3), 813-821.
https://doi.org/10.1007/s00394-013-0585-8

Velickovic K, Markelic M, Golic I, Otašević V, Stančić A, Janković A, Vucetic M, Buzadžić BJ, Korać B, Korac A. Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine. in European Journal of Nutrition. 2014;53(3):813-821.
doi:10.1007/s00394-013-0585-8 .

Velickovic, Ksenija, Markelic, Milica, Golic, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vucetic, Milica, Buzadžić, Biljana J., Korać, Bato, Korac, Aleksandra, "Long-term dietary L-arginine supplementation increases endothelial
 nitric oxide synthase and vasoactive intestinal peptide immunoexpression
 in rat small intestine" in European Journal of Nutrition, 53, no. 3 (2014):813-821,
https://doi.org/10.1007/s00394-013-0585-8 . .

TY  - JOUR
AU  - Janković, Aleksandra
AU  - Korac, Aleksandra
AU  - Srdic-Galic, Biljana
AU  - Buzadžić, Biljana J.
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Vucetic, Milica
AU  - Markelic, Milica
AU  - Velickovic, Ksenija
AU  - Golic, Igor
AU  - Korać, Bato
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2220
AB  - Objective. Metabolic homeostasis depends on adipocyte metabolic
   responses/processes, most of which are redox-regulated. Besides,
   visceral and subcutaneous adipose tissues (VAT and SAT, respectively)
   differ metabolically and in their contribution to metabolic
   complications, but their redox characteristics in humans are still
   unknown. To understand the molecular mechanisms of metabolic syndrome
   development, we analysed the redox characteristics of VAT and SAT in
   groups with various body weights and metabolic risks.
   Material and Methods. Fifty premenopausal women were classified
   according to body mass index into normal-weight and obese groups, and
   these groups were further sub-classified into metabolically healthy and
   metabolically obese ({''}at risk{''}) based on the homeostasis model
   assessment of insulin resistance (HOMA-IR) index and the triglyceride,
   total-, LDL- and HDL-cholesterol levels. Antioxidant components, NADPH
   oxidase protein and 4-hydroxynonenal (4-HNE) levels were analysed in VAT
   and SAT.
   Results. Compared with the SAT, the VAT showed a higher basal level of
   glutathione (GSH) and GSH-dependent enzyme activities. Compared with the
   metabolically healthy normal-weight controls, the obese groups of women
   showed lower GSH levels in both depots. However, in these groups,
   additional prooxidative changes (increased NADPH oxidase and 4-HNE and
   decreased levels of SOD and/or CAT) were observed only in VAT.
   Conclusions. Because of the critical role of thiol-redox homeostasis in
   lipogenesis, interdepot-differences in the GSH-dependent antioxidant
   part may be connected to the higher metabolic activity found in VAT.
   Analogously, the lower GSH levels that occur during obesity and the
   corresponding additional redox imbalance may be signs of VAT metabolic
   dysfunction that underlie the subsequent metabolic impairment. (C) 2014
   Elsevier Inc. All rights reserved.
T2  - Metabolism-Clinical and Experimental
T1  - Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk
IS  - 5
VL  - 63
DO  - 10.1016/j.metabol.2014.01.009
SP  - 661
EP  - 671
ER  - 

@article{
author = "Janković, Aleksandra and Korac, Aleksandra and Srdic-Galic, Biljana and Buzadžić, Biljana J. and Otašević, Vesna and Stančić, Ana and Vucetic, Milica and Markelic, Milica and Velickovic, Ksenija and Golic, Igor and Korać, Bato",
year = "2014",
abstract = "Objective. Metabolic homeostasis depends on adipocyte metabolic
   responses/processes, most of which are redox-regulated. Besides,
   visceral and subcutaneous adipose tissues (VAT and SAT, respectively)
   differ metabolically and in their contribution to metabolic
   complications, but their redox characteristics in humans are still
   unknown. To understand the molecular mechanisms of metabolic syndrome
   development, we analysed the redox characteristics of VAT and SAT in
   groups with various body weights and metabolic risks.
   Material and Methods. Fifty premenopausal women were classified
   according to body mass index into normal-weight and obese groups, and
   these groups were further sub-classified into metabolically healthy and
   metabolically obese ({''}at risk{''}) based on the homeostasis model
   assessment of insulin resistance (HOMA-IR) index and the triglyceride,
   total-, LDL- and HDL-cholesterol levels. Antioxidant components, NADPH
   oxidase protein and 4-hydroxynonenal (4-HNE) levels were analysed in VAT
   and SAT.
   Results. Compared with the SAT, the VAT showed a higher basal level of
   glutathione (GSH) and GSH-dependent enzyme activities. Compared with the
   metabolically healthy normal-weight controls, the obese groups of women
   showed lower GSH levels in both depots. However, in these groups,
   additional prooxidative changes (increased NADPH oxidase and 4-HNE and
   decreased levels of SOD and/or CAT) were observed only in VAT.
   Conclusions. Because of the critical role of thiol-redox homeostasis in
   lipogenesis, interdepot-differences in the GSH-dependent antioxidant
   part may be connected to the higher metabolic activity found in VAT.
   Analogously, the lower GSH levels that occur during obesity and the
   corresponding additional redox imbalance may be signs of VAT metabolic
   dysfunction that underlie the subsequent metabolic impairment. (C) 2014
   Elsevier Inc. All rights reserved.",
journal = "Metabolism-Clinical and Experimental",
title = "Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk",
number = "5",
volume = "63",
doi = "10.1016/j.metabol.2014.01.009",
pages = "661-671"
}

Janković, A., Korac, A., Srdic-Galic, B., Buzadžić, B. J., Otašević, V., Stančić, A., Vucetic, M., Markelic, M., Velickovic, K., Golic, I.,& Korać, B.. (2014). Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk. in Metabolism-Clinical and Experimental, 63(5), 661-671.
https://doi.org/10.1016/j.metabol.2014.01.009

Janković A, Korac A, Srdic-Galic B, Buzadžić BJ, Otašević V, Stančić A, Vucetic M, Markelic M, Velickovic K, Golic I, Korać B. Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk. in Metabolism-Clinical and Experimental. 2014;63(5):661-671.
doi:10.1016/j.metabol.2014.01.009 .

Janković, Aleksandra, Korac, Aleksandra, Srdic-Galic, Biljana, Buzadžić, Biljana J., Otašević, Vesna, Stančić, Ana, Vucetic, Milica, Markelic, Milica, Velickovic, Ksenija, Golic, Igor, Korać, Bato, "Differences in the redox status of human visceral and subcutaneous
 adipose tissues - relationships to obesity and metabolic risk" in Metabolism-Clinical and Experimental, 63, no. 5 (2014):661-671,
https://doi.org/10.1016/j.metabol.2014.01.009 . .

TY  - JOUR
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Klepal, Waltraud
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korac, Aleksandra B
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1030
AB  - The aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role of lipid peroxidation and iron in this process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement in the lipofuscin production, accompanied by an increase of mitochondrial damage in brown adipocytes. Extensive fusions of lipid droplets and mitochondria with lysosomes were also observed. Confocal microscopy showed lipofuscin autofluorescence emission in brown adipose tissue (BAT) after excitation at 488 nm and 633 nm, particularly in the insulin-treated groups. The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4-HNE), in brown adipocytes was assessed by immunohistochemical examination revealing its higher content after treatment with insulin. The iron content was quantified by electron dispersive X-ray analysis (EDX) showing its higher content in the hyperinsulinaemic groups. The ultrastucture of the majority of lipofuscin granules suggests their mitochondrial origin, which was additionally confirmed by their co-localization with ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a consequence of lipid peroxidation, mitochondrial damage and iron accumulation.
T2  - Histology and Histopathology
T1  - The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron
IS  - 4
VL  - 28
SP  - 435
EP  - 503
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1030
ER  - 

@article{
author = "Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Klepal, Waltraud and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Buzadžić, Biljana J. and Korać, Bato and Korac, Aleksandra B",
year = "2013",
abstract = "The aim of this study was to investigate lipofuscin origin in brown adipocytes of hyperinsulinaemic rats and the possible role of lipid peroxidation and iron in this process. Ultrastructural examination revealed hyperinsulinaemia-induced enhancement in the lipofuscin production, accompanied by an increase of mitochondrial damage in brown adipocytes. Extensive fusions of lipid droplets and mitochondria with lysosomes were also observed. Confocal microscopy showed lipofuscin autofluorescence emission in brown adipose tissue (BAT) after excitation at 488 nm and 633 nm, particularly in the insulin-treated groups. The presence and distribution of lipid peroxidation product, 4-hydroxy-2-nonenal (4-HNE), in brown adipocytes was assessed by immunohistochemical examination revealing its higher content after treatment with insulin. The iron content was quantified by electron dispersive X-ray analysis (EDX) showing its higher content in the hyperinsulinaemic groups. The ultrastucture of the majority of lipofuscin granules suggests their mitochondrial origin, which was additionally confirmed by their co-localization with ATP synthase. In conclusion, our results suggest that increased lipofuscinogenesis in the brown adipocytes of hyperinsulinaemic rats is a consequence of lipid peroxidation, mitochondrial damage and iron accumulation.",
journal = "Histology and Histopathology",
title = "The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron",
number = "4",
volume = "28",
pages = "435-503",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1030"
}

Markelić, M. B., Velicković, K. D., Golić, I., Klepal, W., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Buzadžić, B. J., Korać, B.,& Korac, A. B.. (2013). The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron. in Histology and Histopathology, 28(4), 435-503.
https://hdl.handle.net/21.15107/rcub_ibiss_1030

Markelić MB, Velicković KD, Golić I, Klepal W, Otašević V, Stančić A, Janković A, Vučetić M, Buzadžić BJ, Korać B, Korac AB. The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron. in Histology and Histopathology. 2013;28(4):435-503.
https://hdl.handle.net/21.15107/rcub_ibiss_1030 .

Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Klepal, Waltraud, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Buzadžić, Biljana J., Korać, Bato, Korac, Aleksandra B, "The origin of lipofuscin in brown adipocytes of hyperinsulinaemic rats: the role of lipid peroxidation and iron" in Histology and Histopathology, 28, no. 4 (2013):435-503,
https://hdl.handle.net/21.15107/rcub_ibiss_1030 .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Vučetić, Milica
AU  - Macanović, Biljana
AU  - Garalejić, Eliana
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Buzadžić, Biljana J.
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Markelić, Milica B
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1067
AB  - Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((NO)-N-center dot), the number of MitoTracker (R) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (NO)-N-center dot might be involved in the observed effects of the mimic. Antioxid. Redox Signal. 18, 170-178.
T2  - Antioxidants & Redox Signaling
T1  - Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?
IS  - 2
VL  - 18
SP  - 23
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1067
ER  - 

@article{
author = "Otašević, Vesna and Korac, Aleksandra B and Vučetić, Milica and Macanović, Biljana and Garalejić, Eliana and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Buzadžić, Biljana J. and Stančić, Ana and Janković, Aleksandra and Velicković, Ksenija D and Golić, Igor and Markelić, Milica B and Korać, Bato",
year = "2013",
abstract = "Mitochondria play an important role in sperm cell maturation and function. Here, we examined whether (and how) changes in sperm redox milieu affect the functional status of sperm mitochondria, that is, sperm functionality. Compared with the control, incubation in Tyrode's medium for 3 h, under noncapacitating conditions, decreased sperm motility, the amount of nitric oxide ((NO)-N-center dot), the number of MitoTracker (R) Green FM (MT-G) positive mitochondria, and the expression of complexes I and IV of the mitochondrial respiratory chain. In turn, superoxide dismutase (SOD) mimic (M40403) treatment restored/increased these parameters, as well as the expression of endothelial nitric oxide synthase, manganese SOD, and catalase. These data lead to the hypothesis that M40403 improves mitochondrial functional state and motility of spermatozoa, as well as (NO)-N-center dot might be involved in the observed effects of the mimic. Antioxid. Redox Signal. 18, 170-178.",
journal = "Antioxidants & Redox Signaling",
title = "Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?",
number = "2",
volume = "18",
pages = "23-178",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1067"
}

Otašević, V., Korac, A. B., Vučetić, M., Macanović, B., Garalejić, E., Ivanović-Burmazović, I. S., Filipović, M. R., Buzadžić, B. J., Stančić, A., Janković, A., Velicković, K. D., Golić, I., Markelić, M. B.,& Korać, B.. (2013). Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?. in Antioxidants & Redox Signaling, 18(2), 23-178.
https://hdl.handle.net/21.15107/rcub_ibiss_1067

Otašević V, Korac AB, Vučetić M, Macanović B, Garalejić E, Ivanović-Burmazović IS, Filipović MR, Buzadžić BJ, Stančić A, Janković A, Velicković KD, Golić I, Markelić MB, Korać B. Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?. in Antioxidants & Redox Signaling. 2013;18(2):23-178.
https://hdl.handle.net/21.15107/rcub_ibiss_1067 .

Otašević, Vesna, Korac, Aleksandra B, Vučetić, Milica, Macanović, Biljana, Garalejić, Eliana, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Buzadžić, Biljana J., Stančić, Ana, Janković, Aleksandra, Velicković, Ksenija D, Golić, Igor, Markelić, Milica B, Korać, Bato, "Is Manganese (II) Pentaazamacrocyclic Superoxide Dismutase Mimic Beneficial for Human Sperm Mitochondria Function and Motility?" in Antioxidants & Redox Signaling, 18, no. 2 (2013):23-178,
https://hdl.handle.net/21.15107/rcub_ibiss_1067 .

TY  - JOUR
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Korac, Aleksandra B
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/960
AB  - Hippocampal structural changes associated with diabetes-related cognitive impairments are well described, but their molecular background remained vague. We examined whether/how diabetes alters molecular basis of energy metabolism in hippocampus readily after diabetes onset, with special emphasis on its redox-sensitivity. To induce diabetes, adult Mill Hill hybrid hooded rats received a single alloxan dose (120 mg/kg). Both non-diabetic and diabetic groups were further divided in two subgroups receiving (i) or not (ii) superoxide dismutase (SOD) mimic, [Mn(II)(pyane)Cl-2] for 7 days, i.p. Treatment of the diabetic animals started after blood glucose level >= 12 mM. Diabetes decreased protein levels of oxidative phosphorylation components: complex III and ATP synthase. In contrast, protein amounts of glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, and hypoxia-inducible factor-1 alpha - the key regulator of energy metabolism in stress conditions, were higher in diabetic animals. Treatment with SOD mimic restored/increased the levels of oxidative phosphorylation components and returned hypoxia-inducible factor-1 alpha to control level, while diabetes-induced up-regulation of glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, was additionally stimulated. To conclude, our results provide insight into the earliest molecular changes of energy-producing pathways in diabetes that may account for structural/functional disturbance of hippocampus, seen during disease progression. Also, data suggest [Mn(II)(pyane)Cl-2] as potential therapeutic agent in cutting-edge approaches to threat this widespread metabolic disorder. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Brain Research Bulletin
T1  - Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic
IS  - null
VL  - 99
SP  - 153
EP  - 33
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_960
ER  - 

@article{
author = "Stančić, Ana and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Korac, Aleksandra B and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Buzadžić, Biljana J. and Korać, Bato",
year = "2013",
abstract = "Hippocampal structural changes associated with diabetes-related cognitive impairments are well described, but their molecular background remained vague. We examined whether/how diabetes alters molecular basis of energy metabolism in hippocampus readily after diabetes onset, with special emphasis on its redox-sensitivity. To induce diabetes, adult Mill Hill hybrid hooded rats received a single alloxan dose (120 mg/kg). Both non-diabetic and diabetic groups were further divided in two subgroups receiving (i) or not (ii) superoxide dismutase (SOD) mimic, [Mn(II)(pyane)Cl-2] for 7 days, i.p. Treatment of the diabetic animals started after blood glucose level >= 12 mM. Diabetes decreased protein levels of oxidative phosphorylation components: complex III and ATP synthase. In contrast, protein amounts of glyceraldehyde-3-phosphate dehydrogenase, pyruvate dehydrogenase, and hypoxia-inducible factor-1 alpha - the key regulator of energy metabolism in stress conditions, were higher in diabetic animals. Treatment with SOD mimic restored/increased the levels of oxidative phosphorylation components and returned hypoxia-inducible factor-1 alpha to control level, while diabetes-induced up-regulation of glycolytic enzyme, glyceraldehyde-3-phosphate dehydrogenase, was additionally stimulated. To conclude, our results provide insight into the earliest molecular changes of energy-producing pathways in diabetes that may account for structural/functional disturbance of hippocampus, seen during disease progression. Also, data suggest [Mn(II)(pyane)Cl-2] as potential therapeutic agent in cutting-edge approaches to threat this widespread metabolic disorder. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Brain Research Bulletin",
title = "Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic",
number = "null",
volume = "99",
pages = "153-33",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_960"
}

Stančić, A., Otašević, V., Janković, A., Vučetić, M., Ivanović-Burmazović, I. S., Filipović, M. R., Korac, A. B., Markelić, M. B., Velicković, K. D., Golić, I., Buzadžić, B. J.,& Korać, B.. (2013). Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic. in Brain Research Bulletin, 99(null), 153-33.
https://hdl.handle.net/21.15107/rcub_ibiss_960

Stančić A, Otašević V, Janković A, Vučetić M, Ivanović-Burmazović IS, Filipović MR, Korac AB, Markelić MB, Velicković KD, Golić I, Buzadžić BJ, Korać B. Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic. in Brain Research Bulletin. 2013;99(null):153-33.
https://hdl.handle.net/21.15107/rcub_ibiss_960 .

Stančić, Ana, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Korac, Aleksandra B, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Buzadžić, Biljana J., Korać, Bato, "Molecular basis of hippocampal energy metabolism in diabetic rats: The effects of SOD mimic" in Brain Research Bulletin, 99, no. null (2013):153-33,
https://hdl.handle.net/21.15107/rcub_ibiss_960 .

TY  - JOUR
AU  - Stančić, Ana
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/951
AB  - This study examined the molecular basis of energy-related regulatory mechanisms underlying metabolic recruitment of skeletal muscle during cold acclimation and possible involvement of the L-arginine/nitric oxide-producing pathway. Rats exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21 and 45 days were divided into three groups: untreated, L-arginine treated and N-omega-nitro-L-arginine methyl ester (L-NAME) treated. Compared with controls (22 +/- 1 degrees C), there was an initial increase in the protein level of 5'-AMP-activated protein kinase alpha (day 1), followed by an increase in peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) and peroxisome proliferator-activated receptors (PPARs): PPAR alpha and PPAR gamma from day 1 and PPAR delta from day 7 of cold acclimation. Activation of the PGC-1 alpha/PPAR transcription program was accompanied by increased protein expression of the key metabolic enzymes in beta-oxidation, the tricarboxylic acid cycle and oxidative phosphorylation, with the exceptions in complex I (no changes) and ATP synthase (decreased at day 1). Cold did not affect hexokinase and GAPDH protein levels, but increased lactate dehydrogenase activity compared with controls (1-45 days). L-arginine sustained, accelerated and/or intensified cold-induced molecular remodeling throughout cold acclimation. L-NAME exerted phase-dependent effects: similar to L-arginine in early cold acclimation and opposite after prolonged cold exposure (from day 21). It seems that upregulation of the PGC-1 alpha/PPAR transcription program early during cold acclimation triggers the molecular recruitment of skeletal muscle underlying the shift to more oxidative metabolism during prolonged cold acclimation. Our results suggest that nitric oxide has a role in maintaining the skeletal muscle oxidative phenotype in late cold acclimation but question its role early in cold acclimation.
T2  - Journal of Experimental Biology
T1  - Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation
IS  - 22
VL  - 216
SP  - 73
EP  - 4241
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_951
ER  - 

@article{
author = "Stančić, Ana and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Korać, Bato",
year = "2013",
abstract = "This study examined the molecular basis of energy-related regulatory mechanisms underlying metabolic recruitment of skeletal muscle during cold acclimation and possible involvement of the L-arginine/nitric oxide-producing pathway. Rats exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21 and 45 days were divided into three groups: untreated, L-arginine treated and N-omega-nitro-L-arginine methyl ester (L-NAME) treated. Compared with controls (22 +/- 1 degrees C), there was an initial increase in the protein level of 5'-AMP-activated protein kinase alpha (day 1), followed by an increase in peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1 alpha) and peroxisome proliferator-activated receptors (PPARs): PPAR alpha and PPAR gamma from day 1 and PPAR delta from day 7 of cold acclimation. Activation of the PGC-1 alpha/PPAR transcription program was accompanied by increased protein expression of the key metabolic enzymes in beta-oxidation, the tricarboxylic acid cycle and oxidative phosphorylation, with the exceptions in complex I (no changes) and ATP synthase (decreased at day 1). Cold did not affect hexokinase and GAPDH protein levels, but increased lactate dehydrogenase activity compared with controls (1-45 days). L-arginine sustained, accelerated and/or intensified cold-induced molecular remodeling throughout cold acclimation. L-NAME exerted phase-dependent effects: similar to L-arginine in early cold acclimation and opposite after prolonged cold exposure (from day 21). It seems that upregulation of the PGC-1 alpha/PPAR transcription program early during cold acclimation triggers the molecular recruitment of skeletal muscle underlying the shift to more oxidative metabolism during prolonged cold acclimation. Our results suggest that nitric oxide has a role in maintaining the skeletal muscle oxidative phenotype in late cold acclimation but question its role early in cold acclimation.",
journal = "Journal of Experimental Biology",
title = "Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation",
number = "22",
volume = "216",
pages = "73-4241",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_951"
}

Stančić, A., Buzadžić, B. J., Korac, A. B., Otašević, V., Janković, A., Vučetić, M., Markelić, M. B., Velicković, K. D., Golić, I.,& Korać, B.. (2013). Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation. in Journal of Experimental Biology, 216(22), 73-4241.
https://hdl.handle.net/21.15107/rcub_ibiss_951

Stančić A, Buzadžić BJ, Korac AB, Otašević V, Janković A, Vučetić M, Markelić MB, Velicković KD, Golić I, Korać B. Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation. in Journal of Experimental Biology. 2013;216(22):73-4241.
https://hdl.handle.net/21.15107/rcub_ibiss_951 .

Stančić, Ana, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Korać, Bato, "Regulatory role of PGC-1 alpha/PPAR signaling in skeletal muscle metabolic recruitment during cold acclimation" in Journal of Experimental Biology, 216, no. 22 (2013):73-4241,
https://hdl.handle.net/21.15107/rcub_ibiss_951 .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Korac, Aleksandra B
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Buzadžić, Biljana J.
AU  - Storey, Kenneth B
AU  - Korać, Bato
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/944
AB  - Any alteration in oxidative metabolism is coupled with a corresponding response by an antioxidant defense (AD) in appropriate subcellular compartments. Seasonal hibernators pass through circannual metabolic adaptations that allow them to either maintain euthermy (cold acclimation) or enter winter torpor with body temperature falling to low values. The present study aimed to investigate the corresponding pattern of AD enzyme protein expressions associated with these strategies in the main tissues involved in whole animal energy homeostasis: brown and white adipose tissues (BAT and WAT, respectively), liver, and skeletal muscle. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 +/- 1 C) and then divided into two groups: (1) animals fell into torpor (hibernating group) and (2) animals stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). We examined the effects of cold acclimation and hibernation on the tissue-dependent protein expression of four enzymes which catalyze the two-step detoxification of superoxide to water: superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), and glutathione peroxidase (GSH-Px). The results showed that hibernation induced an increase of AD enzyme protein expressions in BAT and skeletal muscle. However, AD enzyme contents in liver were largely unaffected during torpor. Under these conditions, different WAT depots responded by elevating the amounts of specific enzymes, as follows: SOD 1 in retroperitoneal WAT, GSH-Px in gonadal WAT, and CAT in subcutaneous WAT. Similar perturbations of AD enzymes contents were seen in all tissues during cold acclimation, often in a time-dependent manner. It can be concluded that BAT and muscle AD capacity undergo the most dramatic changes during both cold acclimation and hibernation, while liver is relatively unaffected by either condition. Additionally, this study provides a basis for further metabolic study that will illuminate the causes of these tissue-specific AD responses, particularly the novel finding of distinct responses by different WAT depots in hibernators. (C) 2013 Elsevier Inc. All rights reserved.
T2  - Free Radical Biology and Medicine
T1  - The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update
IS  - null
VL  - 65
SP  - 47
EP  - 924
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_944
ER  - 

@article{
author = "Vučetić, Milica and Stančić, Ana and Otašević, Vesna and Janković, Aleksandra and Korac, Aleksandra B and Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Buzadžić, Biljana J. and Storey, Kenneth B and Korać, Bato",
year = "2013",
abstract = "Any alteration in oxidative metabolism is coupled with a corresponding response by an antioxidant defense (AD) in appropriate subcellular compartments. Seasonal hibernators pass through circannual metabolic adaptations that allow them to either maintain euthermy (cold acclimation) or enter winter torpor with body temperature falling to low values. The present study aimed to investigate the corresponding pattern of AD enzyme protein expressions associated with these strategies in the main tissues involved in whole animal energy homeostasis: brown and white adipose tissues (BAT and WAT, respectively), liver, and skeletal muscle. European ground squirrels (Spermophilus citellus) were exposed to low temperature (4 +/- 1 C) and then divided into two groups: (1) animals fell into torpor (hibernating group) and (2) animals stayed active and euthermic for 1, 3, 7, 12, or 21 days (cold-exposed group). We examined the effects of cold acclimation and hibernation on the tissue-dependent protein expression of four enzymes which catalyze the two-step detoxification of superoxide to water: superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), and glutathione peroxidase (GSH-Px). The results showed that hibernation induced an increase of AD enzyme protein expressions in BAT and skeletal muscle. However, AD enzyme contents in liver were largely unaffected during torpor. Under these conditions, different WAT depots responded by elevating the amounts of specific enzymes, as follows: SOD 1 in retroperitoneal WAT, GSH-Px in gonadal WAT, and CAT in subcutaneous WAT. Similar perturbations of AD enzymes contents were seen in all tissues during cold acclimation, often in a time-dependent manner. It can be concluded that BAT and muscle AD capacity undergo the most dramatic changes during both cold acclimation and hibernation, while liver is relatively unaffected by either condition. Additionally, this study provides a basis for further metabolic study that will illuminate the causes of these tissue-specific AD responses, particularly the novel finding of distinct responses by different WAT depots in hibernators. (C) 2013 Elsevier Inc. All rights reserved.",
journal = "Free Radical Biology and Medicine",
title = "The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update",
number = "null",
volume = "65",
pages = "47-924",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_944"
}

Vučetić, M., Stančić, A., Otašević, V., Janković, A., Korac, A. B., Markelić, M. B., Velicković, K. D., Golić, I., Buzadžić, B. J., Storey, K. B.,& Korać, B.. (2013). The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update. in Free Radical Biology and Medicine, 65(null), 47-924.
https://hdl.handle.net/21.15107/rcub_ibiss_944

Vučetić M, Stančić A, Otašević V, Janković A, Korac AB, Markelić MB, Velicković KD, Golić I, Buzadžić BJ, Storey KB, Korać B. The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update. in Free Radical Biology and Medicine. 2013;65(null):47-924.
https://hdl.handle.net/21.15107/rcub_ibiss_944 .

Vučetić, Milica, Stančić, Ana, Otašević, Vesna, Janković, Aleksandra, Korac, Aleksandra B, Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Buzadžić, Biljana J., Storey, Kenneth B, Korać, Bato, "The impact of cold acclimation and hibernation on antioxidant defenses in the ground squirrel (Spermophilus citellus): An update" in Free Radical Biology and Medicine, 65, no. null (2013):47-924,
https://hdl.handle.net/21.15107/rcub_ibiss_944 .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Markelić, Milica B
AU  - Golić, Igor
AU  - Velicković, Ksenija D
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1148
AB  - In this study, the effects of l-arginine-nitric-oxide ((NO)-N-a (TM))-producing pathway on protein content of ubiquitin, as an important component of ubiquitin-proteasome system for protein removal, were investigated. We showed that l-arginine markedly decreased ubiquitin protein content in interscapular brown adipose tissue, both in thermogenic inactive (at room temperature) and thermogenic active (on cold) states; while in l-NAME-treated groups this effect was abolished. This result suggests that nitric oxide ((NO)-N-a (TM)), besides well established roles, is involved in this aspect of structure remodeling, as well.
T2  - Molecular and Cellular Biochemistry
T1  - Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot
IS  - 1-2
VL  - 368
SP  - 503
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1148
ER  - 

@article{
author = "Vučetić, Milica and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Markelić, Milica B and Golić, Igor and Velicković, Ksenija D and Buzadžić, Biljana J. and Korac, Aleksandra B and Korać, Bato",
year = "2012",
abstract = "In this study, the effects of l-arginine-nitric-oxide ((NO)-N-a (TM))-producing pathway on protein content of ubiquitin, as an important component of ubiquitin-proteasome system for protein removal, were investigated. We showed that l-arginine markedly decreased ubiquitin protein content in interscapular brown adipose tissue, both in thermogenic inactive (at room temperature) and thermogenic active (on cold) states; while in l-NAME-treated groups this effect was abolished. This result suggests that nitric oxide ((NO)-N-a (TM)), besides well established roles, is involved in this aspect of structure remodeling, as well.",
journal = "Molecular and Cellular Biochemistry",
title = "Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot",
number = "1-2",
volume = "368",
pages = "503-193",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1148"
}

Vučetić, M., Otašević, V., Stančić, A., Janković, A., Markelić, M. B., Golić, I., Velicković, K. D., Buzadžić, B. J., Korac, A. B.,& Korać, B.. (2012). Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot. in Molecular and Cellular Biochemistry, 368(1-2), 503-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1148

Vučetić M, Otašević V, Stančić A, Janković A, Markelić MB, Golić I, Velicković KD, Buzadžić BJ, Korac AB, Korać B. Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot. in Molecular and Cellular Biochemistry. 2012;368(1-2):503-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1148 .

Vučetić, Milica, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Markelić, Milica B, Golić, Igor, Velicković, Ksenija D, Buzadžić, Biljana J., Korac, Aleksandra B, Korać, Bato, "Protein expression of ubiquitin in interscapular brown adipose tissue during acclimation of rats to cold: the impact of (NO)-N-center dot" in Molecular and Cellular Biochemistry, 368, no. 1-2 (2012):503-193,
https://hdl.handle.net/21.15107/rcub_ibiss_1148 .

TY  - JOUR
AU  - Jović, Miomir Đ
AU  - Stančić, Ana
AU  - Nenadić, Dragan
AU  - Cekić, Olivera
AU  - Nezić, Dusko G
AU  - Milojević, Predrag S
AU  - Micović, Slobodan V
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1138
AB  - Background/Aims: Study elucidates and compares the mitochondrial bioenergetic-related molecular basis of sevoflurane and propofol cardioprotection during aortic valve replacement surgery due to aortic valve stenosis. Methods: Twenty-two patients were prospectively randomized in two groups regarding the anesthetic regime: sevoflurane and propofol. Hemodynamic parameters, biomarkers of cardiac injury and brain natriuretic peptide (BNP) were measured preoperatively and postoperatively. In tissue samples, taken from the interventricular septum, key mitochondrial molecules were determined by Western blot, real time PCR, as well as confocal microscopy and immunohisto- and immunocyto-chemical analysis. Results: The protein levels of cytochrome c oxidase and ATP synthase were higher in sevoflurane than in propofol group. Nevertheless, cytochrome c protein content was higher in propofol than sevoflurane receiving patients. Propofol group also showed higher protein level of connexin 43 (Cx43) than sevoflurane group. Besides, immunogold analysis showed its mitochondrial localization. The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. On the other hand, there were no significant differences between groups in hemodynamic assessment, intensive care unit length of stay, troponin I and BNP level. Conclusions: Our data indicate that sevoflurane and propofol lead to cardiac protection via different mitochondrially related molecular mechanisms. It appears that sevoflurane acts regulating cytochrome c oxidase and ATP synthase, while the effects of propofol occur through regulation of cytochrome c, Cx43, mtDNA transcription and UCP2. Copyright (C) 2012 S. Karger AG, Basel
T2  - Cellular Physiology and Biochemistry
T1  - Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass
IS  - 1-2
VL  - 29
SP  - 973
EP  - 142
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1138
ER  - 

@article{
author = "Jović, Miomir Đ and Stančić, Ana and Nenadić, Dragan and Cekić, Olivera and Nezić, Dusko G and Milojević, Predrag S and Micović, Slobodan V and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Velicković, Ksenija D and Golić, Igor and Korać, Bato",
year = "2012",
abstract = "Background/Aims: Study elucidates and compares the mitochondrial bioenergetic-related molecular basis of sevoflurane and propofol cardioprotection during aortic valve replacement surgery due to aortic valve stenosis. Methods: Twenty-two patients were prospectively randomized in two groups regarding the anesthetic regime: sevoflurane and propofol. Hemodynamic parameters, biomarkers of cardiac injury and brain natriuretic peptide (BNP) were measured preoperatively and postoperatively. In tissue samples, taken from the interventricular septum, key mitochondrial molecules were determined by Western blot, real time PCR, as well as confocal microscopy and immunohisto- and immunocyto-chemical analysis. Results: The protein levels of cytochrome c oxidase and ATP synthase were higher in sevoflurane than in propofol group. Nevertheless, cytochrome c protein content was higher in propofol than sevoflurane receiving patients. Propofol group also showed higher protein level of connexin 43 (Cx43) than sevoflurane group. Besides, immunogold analysis showed its mitochondrial localization. The mRNA level of mtDNA and uncoupling protein (UCP2) were higher in propofol than sevoflurane patients, as well. On the other hand, there were no significant differences between groups in hemodynamic assessment, intensive care unit length of stay, troponin I and BNP level. Conclusions: Our data indicate that sevoflurane and propofol lead to cardiac protection via different mitochondrially related molecular mechanisms. It appears that sevoflurane acts regulating cytochrome c oxidase and ATP synthase, while the effects of propofol occur through regulation of cytochrome c, Cx43, mtDNA transcription and UCP2. Copyright (C) 2012 S. Karger AG, Basel",
journal = "Cellular Physiology and Biochemistry",
title = "Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass",
number = "1-2",
volume = "29",
pages = "973-142",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1138"
}

Jović, M. Đ., Stančić, A., Nenadić, D., Cekić, O., Nezić, D. G., Milojević, P. S., Micović, S. V., Buzadžić, B. J., Korac, A. B., Otašević, V., Janković, A., Vučetić, M., Velicković, K. D., Golić, I.,& Korać, B.. (2012). Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass. in Cellular Physiology and Biochemistry, 29(1-2), 973-142.
https://hdl.handle.net/21.15107/rcub_ibiss_1138

Jović MĐ, Stančić A, Nenadić D, Cekić O, Nezić DG, Milojević PS, Micović SV, Buzadžić BJ, Korac AB, Otašević V, Janković A, Vučetić M, Velicković KD, Golić I, Korać B. Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass. in Cellular Physiology and Biochemistry. 2012;29(1-2):973-142.
https://hdl.handle.net/21.15107/rcub_ibiss_1138 .

Jović, Miomir Đ, Stančić, Ana, Nenadić, Dragan, Cekić, Olivera, Nezić, Dusko G, Milojević, Predrag S, Micović, Slobodan V, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Velicković, Ksenija D, Golić, Igor, Korać, Bato, "Mitochondrial Molecular Basis of Sevoflurane and Propofol Cardioprotection in Patients Undergoing Aortic Valve Replacement with Cardiopulmonary Bypass" in Cellular Physiology and Biochemistry, 29, no. 1-2 (2012):973-142,
https://hdl.handle.net/21.15107/rcub_ibiss_1138 .

TY  - CONF
AU  - Vučetić, Milica
AU  - Stančić, Ana
AU  - Filipović, Milos R
AU  - Ivanović-Burmazović, Ivana S
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Janković, Aleksandra
AU  - Buzadžić, Biljana J.
AU  - Velicković, Ksenija D
AU  - Markelić, Milica B
AU  - Golić, Igor
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1137
C3  - European Journal of Clinical Investigation
T1  - The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats
IS  - null
VL  - 42
SP  - 969
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1137
ER  - 

@conference{
author = "Vučetić, Milica and Stančić, Ana and Filipović, Milos R and Ivanović-Burmazović, Ivana S and Otašević, Vesna and Korac, Aleksandra B and Janković, Aleksandra and Buzadžić, Biljana J. and Velicković, Ksenija D and Markelić, Milica B and Golić, Igor and Korać, Bato",
year = "2012",
journal = "European Journal of Clinical Investigation",
title = "The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats",
number = "null",
volume = "42",
pages = "969-76",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1137"
}

Vučetić, M., Stančić, A., Filipović, M. R., Ivanović-Burmazović, I. S., Otašević, V., Korac, A. B., Janković, A., Buzadžić, B. J., Velicković, K. D., Markelić, M. B., Golić, I.,& Korać, B.. (2012). The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats. in European Journal of Clinical Investigation, 42(null), 969-76.
https://hdl.handle.net/21.15107/rcub_ibiss_1137

Vučetić M, Stančić A, Filipović MR, Ivanović-Burmazović IS, Otašević V, Korac AB, Janković A, Buzadžić BJ, Velicković KD, Markelić MB, Golić I, Korać B. The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats. in European Journal of Clinical Investigation. 2012;42(null):969-76.
https://hdl.handle.net/21.15107/rcub_ibiss_1137 .

Vučetić, Milica, Stančić, Ana, Filipović, Milos R, Ivanović-Burmazović, Ivana S, Otašević, Vesna, Korac, Aleksandra B, Janković, Aleksandra, Buzadžić, Biljana J., Velicković, Ksenija D, Markelić, Milica B, Golić, Igor, Korać, Bato, "The effects of superoxide dismutase mimic on energy metabolism in hippocampus of diabetic rats" in European Journal of Clinical Investigation, 42, no. null (2012):969-76,
https://hdl.handle.net/21.15107/rcub_ibiss_1137 .

TY  - JOUR
AU  - Stančić, Ana
AU  - Rasić-Milutinović, Zorica R
AU  - Perunicić-Peković, Gordana B
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Otašević, Vesna
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1189
AB  - Diabetes and renal insufficiency are interrelated metabolic disorders closely associated with redox homeostasis disturbances. The aim of this study was to compare the activity of copper zinc,superoxide dismutase (CuZnSOD) in the erythrocytes of hypertensive diabetic patients with or without renal insufficiency with normal healthy control subjects. In both groups of diabetic patients, blood glucose level and the content of glycosylated hemoglobin (HbA(lc)) were higher than in the control group. However, CuZnSOD activity was significantly higher than control only in hypertensive diabetic patients with renal insufficiency. Our results suggest that disturbances in superoxide homeostasis do correlate with long-term complication in diabetes, i.e. diabetic renal insufficiency and hypertension.
T2  - Indian Journal of Biochemistry & Biophysics
T1  - Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients
IS  - 2
VL  - 49
EP  - 100
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1189
ER  - 

@article{
author = "Stančić, Ana and Rasić-Milutinović, Zorica R and Perunicić-Peković, Gordana B and Buzadžić, Biljana J. and Korac, Aleksandra B and Otašević, Vesna and Janković, Aleksandra and Vučetić, Milica and Korać, Bato",
year = "2012",
abstract = "Diabetes and renal insufficiency are interrelated metabolic disorders closely associated with redox homeostasis disturbances. The aim of this study was to compare the activity of copper zinc,superoxide dismutase (CuZnSOD) in the erythrocytes of hypertensive diabetic patients with or without renal insufficiency with normal healthy control subjects. In both groups of diabetic patients, blood glucose level and the content of glycosylated hemoglobin (HbA(lc)) were higher than in the control group. However, CuZnSOD activity was significantly higher than control only in hypertensive diabetic patients with renal insufficiency. Our results suggest that disturbances in superoxide homeostasis do correlate with long-term complication in diabetes, i.e. diabetic renal insufficiency and hypertension.",
journal = "Indian Journal of Biochemistry & Biophysics",
title = "Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients",
number = "2",
volume = "49",
pages = "100",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1189"
}

Stančić, A., Rasić-Milutinović, Z. R., Perunicić-Peković, G. B., Buzadžić, B. J., Korac, A. B., Otašević, V., Janković, A., Vučetić, M.,& Korać, B.. (2012). Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients. in Indian Journal of Biochemistry & Biophysics, 49(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1189

Stančić A, Rasić-Milutinović ZR, Perunicić-Peković GB, Buzadžić BJ, Korac AB, Otašević V, Janković A, Vučetić M, Korać B. Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients. in Indian Journal of Biochemistry & Biophysics. 2012;49(2):null-100.
https://hdl.handle.net/21.15107/rcub_ibiss_1189 .

Stančić, Ana, Rasić-Milutinović, Zorica R, Perunicić-Peković, Gordana B, Buzadžić, Biljana J., Korac, Aleksandra B, Otašević, Vesna, Janković, Aleksandra, Vučetić, Milica, Korać, Bato, "Relation of CuZnSOD activity with renal insufficiency in hypertensive diabetic patients" in Indian Journal of Biochemistry & Biophysics, 49, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1189 .

TY  - CONF
AU  - Macanović, Biljana
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Vučetić, Milica
AU  - Garalejić, Eliana
AU  - Ivanović-Burmazović, Ivana S
AU  - Filipović, Milos R
AU  - Buzadžić, Biljana J.
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Markelić, Milica B
AU  - Korać, Bato
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1212
C3  - Human Reproduction
T1  - Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO
IS  - null
VL  - 27
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1212
ER  - 

@conference{
author = "Macanović, Biljana and Otašević, Vesna and Korac, Aleksandra B and Vučetić, Milica and Garalejić, Eliana and Ivanović-Burmazović, Ivana S and Filipović, Milos R and Buzadžić, Biljana J. and Stančić, Ana and Janković, Aleksandra and Velicković, Ksenija D and Golić, Igor and Markelić, Milica B and Korać, Bato",
year = "2012",
journal = "Human Reproduction",
title = "Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO",
number = "null",
volume = "27",
pages = "na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1212"
}

Macanović, B., Otašević, V., Korac, A. B., Vučetić, M., Garalejić, E., Ivanović-Burmazović, I. S., Filipović, M. R., Buzadžić, B. J., Stančić, A., Janković, A., Velicković, K. D., Golić, I., Markelić, M. B.,& Korać, B.. (2012). Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO. in Human Reproduction, 27(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1212

Macanović B, Otašević V, Korac AB, Vučetić M, Garalejić E, Ivanović-Burmazović IS, Filipović MR, Buzadžić BJ, Stančić A, Janković A, Velicković KD, Golić I, Markelić MB, Korać B. Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO. in Human Reproduction. 2012;27(null):null-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1212 .

Macanović, Biljana, Otašević, Vesna, Korac, Aleksandra B, Vučetić, Milica, Garalejić, Eliana, Ivanović-Burmazović, Ivana S, Filipović, Milos R, Buzadžić, Biljana J., Stančić, Ana, Janković, Aleksandra, Velicković, Ksenija D, Golić, Igor, Markelić, Milica B, Korać, Bato, "Manganese (II) pentaazamacrocyclic SOD mimic improves functional status of human sperm mitochondria: involvement of iNO" in Human Reproduction, 27, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1212 .

TY  - JOUR
AU  - Buzadžić, Biljana J.
AU  - Vučetić, Milica
AU  - Janković, Aleksandra
AU  - Stančić, Ana
AU  - Otašević, Vesna
AU  - Korać, Bato
PY  - 2011
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/455
AB  - Oxidants, antioxidants and free radicals are terms that are associated with daily supplementation of antioxidants in the prevention of a wide range of diseases and pathology. Unfortunately, that kind of thinking, followed by insufficient knowledge of the redox mechanism, popularistically categorizes oxidants and antioxidants as bad and good. This paper is an attempt to examine their role in biological systems with a central place of redox regulation as a life compromise between these two extremes, based on previous knowledge. A try to get a perspective of the protective role of antioxidant and oxidant toxicity, and to perceive a reality in which their activity and/ or concentration in cells determines their role from pathology to the regulation of physiological processes. A special place in that reality is dedicated to gasotransmitters: nitric monoxide (NO), carbon monox­ide (CO) and hydrogen sulfide (H2S). .
AB  - Oksidanti, antioksidanti i slobodni radikali su termini koji se svakodnevno povezuju sa suplementacijom antioksidanata u prevenciji širokog spektra oboljenja i patologija. Nažalost, takav način razmišljanja, praćen nedovoljnim poznavanjem redoks mehanizama, popularistički kategorizuje oksidante i antioksidante na dobre i loše. Ovaj rad je pokušaj da se na bazi dosadašnjih saznanja sagleda njihova uloga u biološkim sistemima sa centralnim mestom redoks regulacije kao životnog kompromisa između ove dve krajnosti. Pokušaj da se između zaštitne uloge antioksidanata i toksičnosti oksidanata sagleda realnost u kojoj njihova aktivnost i/ili koncentracija u ćelijama determiniše njihovu ulogu od patologije do regulacije fizioloških procesa. Posebno mesto u toj realnosti posvećeno je gasotransmiterima: azot monoksidu (NO), ugljen monoksidu (CO) i vodonik sulfidu (H2S). .
T2  - Hrana i ishrana
T1  - Od Fentonove reakcije do savremenog koncepta redoks regulacije
T1  - From Fenton's reaction to modern concept of redox regulation
IS  - 1
VL  - 52
SP  - 1
EP  - 10
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_455
ER  - 

@article{
author = "Buzadžić, Biljana J. and Vučetić, Milica and Janković, Aleksandra and Stančić, Ana and Otašević, Vesna and Korać, Bato",
year = "2011, 2011",
abstract = "Oxidants, antioxidants and free radicals are terms that are associated with daily supplementation of antioxidants in the prevention of a wide range of diseases and pathology. Unfortunately, that kind of thinking, followed by insufficient knowledge of the redox mechanism, popularistically categorizes oxidants and antioxidants as bad and good. This paper is an attempt to examine their role in biological systems with a central place of redox regulation as a life compromise between these two extremes, based on previous knowledge. A try to get a perspective of the protective role of antioxidant and oxidant toxicity, and to perceive a reality in which their activity and/ or concentration in cells determines their role from pathology to the regulation of physiological processes. A special place in that reality is dedicated to gasotransmitters: nitric monoxide (NO), carbon monox­ide (CO) and hydrogen sulfide (H2S). ., Oksidanti, antioksidanti i slobodni radikali su termini koji se svakodnevno povezuju sa suplementacijom antioksidanata u prevenciji širokog spektra oboljenja i patologija. Nažalost, takav način razmišljanja, praćen nedovoljnim poznavanjem redoks mehanizama, popularistički kategorizuje oksidante i antioksidante na dobre i loše. Ovaj rad je pokušaj da se na bazi dosadašnjih saznanja sagleda njihova uloga u biološkim sistemima sa centralnim mestom redoks regulacije kao životnog kompromisa između ove dve krajnosti. Pokušaj da se između zaštitne uloge antioksidanata i toksičnosti oksidanata sagleda realnost u kojoj njihova aktivnost i/ili koncentracija u ćelijama determiniše njihovu ulogu od patologije do regulacije fizioloških procesa. Posebno mesto u toj realnosti posvećeno je gasotransmiterima: azot monoksidu (NO), ugljen monoksidu (CO) i vodonik sulfidu (H2S). .",
journal = "Hrana i ishrana",
title = "Od Fentonove reakcije do savremenog koncepta redoks regulacije, From Fenton's reaction to modern concept of redox regulation",
number = "1",
volume = "52",
pages = "1-10",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_455"
}

Buzadžić, B. J., Vučetić, M., Janković, A., Stančić, A., Otašević, V.,& Korać, B.. (2011). Od Fentonove reakcije do savremenog koncepta redoks regulacije. in Hrana i ishrana, 52(1), 1-10.
https://hdl.handle.net/21.15107/rcub_ibiss_455

Buzadžić BJ, Vučetić M, Janković A, Stančić A, Otašević V, Korać B. Od Fentonove reakcije do savremenog koncepta redoks regulacije. in Hrana i ishrana. 2011;52(1):1-10.
https://hdl.handle.net/21.15107/rcub_ibiss_455 .

Buzadžić, Biljana J., Vučetić, Milica, Janković, Aleksandra, Stančić, Ana, Otašević, Vesna, Korać, Bato, "Od Fentonove reakcije do savremenog koncepta redoks regulacije" in Hrana i ishrana, 52, no. 1 (2011):1-10,
https://hdl.handle.net/21.15107/rcub_ibiss_455 .

TY  - JOUR
AU  - Vučetić, Milica
AU  - Otašević, Vesna
AU  - Korac, Aleksandra B
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Markelić, Milica B
AU  - Golić, Igor
AU  - Velicković, Ksenija D
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1243
AB  - Background: Brown adipose tissue thermogenic program includes complex molecular and structural changes. However, energetic aspects of this process are poorly depicted. Methods: We investigated time-dependent reprogramming of interscapular brown adipose tissue (IBAT) energy metabolism during cold-acclimation, as well as the effects of nitric oxide ((center dot)NO) on those changes. Rats were exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21. and 45 days, and divided into three groups: control, treated with L-arginine, and treated with N(omega)-nitro-L-arginine methyl ester (L-NAME). Results: In the early phase of cold-acclimation (up to 7 days), the protein levels of all metabolic parameters and oxidative phosphorylation components were below the control. However, metabolic parameters and respiratory chain components entered a new homeostatic level in the late phase of cold-acclimation. These changes were accompanied with increased protein levels of phospho-AMP-dependent protein kinase-alpha (phospho-AMPK alpha) on the first day of cold-acclimation, and hypoxia-inducible factor-1 alpha (HIF-1 alpha) throughout early cold-acclimation. L-arginine positively affected protein expression of enzymes involved in glucose metabolism and beta-oxidation of fatty acids in the early phase of cold-acclimation, and oxidative phosphorylation components throughout cold-acclimation. In contrast, L-NAME had the opposite effects. Conclusion: Results suggest that IBAT structural remodeling is followed by energy metabolism reprogramming, which control might be orchestrated by the action of AMPK alpha and HIF-1 alpha. Data also indicated the involvement of L-arginine-(center dot)NO in the regulation of IBAT metabolism. General significance: Results obtained in this study might be of great importance for elucidating regulatory pathways governing energy metabolism in both physiological and pathophysiological states. (C) 2011 Elsevier B.V. All rights reserved.
T2  - Biochimica et Biophysica Acta-General Subjects
T1  - Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha
IS  - 12
VL  - 1810
EP  - 1261
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1243
ER  - 

@article{
author = "Vučetić, Milica and Otašević, Vesna and Korac, Aleksandra B and Stančić, Ana and Janković, Aleksandra and Markelić, Milica B and Golić, Igor and Velicković, Ksenija D and Buzadžić, Biljana J. and Korać, Bato",
year = "2011",
abstract = "Background: Brown adipose tissue thermogenic program includes complex molecular and structural changes. However, energetic aspects of this process are poorly depicted. Methods: We investigated time-dependent reprogramming of interscapular brown adipose tissue (IBAT) energy metabolism during cold-acclimation, as well as the effects of nitric oxide ((center dot)NO) on those changes. Rats were exposed to cold (4 +/- 1 degrees C) for periods of 1, 3, 7, 12, 21. and 45 days, and divided into three groups: control, treated with L-arginine, and treated with N(omega)-nitro-L-arginine methyl ester (L-NAME). Results: In the early phase of cold-acclimation (up to 7 days), the protein levels of all metabolic parameters and oxidative phosphorylation components were below the control. However, metabolic parameters and respiratory chain components entered a new homeostatic level in the late phase of cold-acclimation. These changes were accompanied with increased protein levels of phospho-AMP-dependent protein kinase-alpha (phospho-AMPK alpha) on the first day of cold-acclimation, and hypoxia-inducible factor-1 alpha (HIF-1 alpha) throughout early cold-acclimation. L-arginine positively affected protein expression of enzymes involved in glucose metabolism and beta-oxidation of fatty acids in the early phase of cold-acclimation, and oxidative phosphorylation components throughout cold-acclimation. In contrast, L-NAME had the opposite effects. Conclusion: Results suggest that IBAT structural remodeling is followed by energy metabolism reprogramming, which control might be orchestrated by the action of AMPK alpha and HIF-1 alpha. Data also indicated the involvement of L-arginine-(center dot)NO in the regulation of IBAT metabolism. General significance: Results obtained in this study might be of great importance for elucidating regulatory pathways governing energy metabolism in both physiological and pathophysiological states. (C) 2011 Elsevier B.V. All rights reserved.",
journal = "Biochimica et Biophysica Acta-General Subjects",
title = "Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha",
number = "12",
volume = "1810",
pages = "1261",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1243"
}

Vučetić, M., Otašević, V., Korac, A. B., Stančić, A., Janković, A., Markelić, M. B., Golić, I., Velicković, K. D., Buzadžić, B. J.,& Korać, B.. (2011). Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha. in Biochimica et Biophysica Acta-General Subjects, 1810(12).
https://hdl.handle.net/21.15107/rcub_ibiss_1243

Vučetić M, Otašević V, Korac AB, Stančić A, Janković A, Markelić MB, Golić I, Velicković KD, Buzadžić BJ, Korać B. Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha. in Biochimica et Biophysica Acta-General Subjects. 2011;1810(12):null-1261.
https://hdl.handle.net/21.15107/rcub_ibiss_1243 .

Vučetić, Milica, Otašević, Vesna, Korac, Aleksandra B, Stančić, Ana, Janković, Aleksandra, Markelić, Milica B, Golić, Igor, Velicković, Ksenija D, Buzadžić, Biljana J., Korać, Bato, "Interscapular brown adipose tissue metabolic reprogramming during cold acclimation: Interplay of HIF-1 alpha and AMPK alpha" in Biochimica et Biophysica Acta-General Subjects, 1810, no. 12 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1243 .

TY  - JOUR
AU  - Otašević, Vesna
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Korać, Bato
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1281
AB  - Study elucidates molecular mechanisms underlying activation of catalase and glutathione peroxidase (GSH-Px) during cold-exposure by examining time-dependent changes in their mRNA, protein expression and activity, in interscapular brown adipose tissue (IBAT) of rats kept at room temperature (22 +/- 1 degrees C), or subjected to cold (4 +/- 1 degrees C) for 1, 3, 7, 12, 21 and 45 days. To examine involvement of nitric oxide (*NO) in catalase and GSH-Px regulation, rats were treated with L-arginine or N((1))-nitro-L-arginine-methyl ester (L-NAME). In all groups, cold increased catalase mRNA, protein expression and activity from day 3 to day 45 of acclimation. However, cold-acclimation increased GSH-Px mRNA content on day 3, protein content from day 1 to day 45 and activity no sooner than on day 45. At room temperature, 21- and 45-day L-arginine and L-NAME supplementation increased catalase and GSH-Px mRNA expression, but decreased their activity. Concomitantly, catalase protein content decreased, while GSH-Px protein expression increased compared to control. Data show that cold is dominant stimulus that determines IBAT catalase and GSH-Px responses, by setting tissue metabolic state. In thermogenic-active tissue, catalase is regulated on transcriptional level, while GSH-Px is regulated post-translationally. On the other side, it was shown that *NO affects catalase and GSH-Px only in thermogenic-inactive tissue. (C) 2011 Elsevier Ltd. All rights reserved.
T2  - Journal of Thermal Biology
T1  - Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures
IS  - 5
VL  - 36
EP  - 276
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1281
ER  - 

@article{
author = "Otašević, Vesna and Buzadžić, Biljana J. and Korac, Aleksandra B and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Korać, Bato",
year = "2011",
abstract = "Study elucidates molecular mechanisms underlying activation of catalase and glutathione peroxidase (GSH-Px) during cold-exposure by examining time-dependent changes in their mRNA, protein expression and activity, in interscapular brown adipose tissue (IBAT) of rats kept at room temperature (22 +/- 1 degrees C), or subjected to cold (4 +/- 1 degrees C) for 1, 3, 7, 12, 21 and 45 days. To examine involvement of nitric oxide (*NO) in catalase and GSH-Px regulation, rats were treated with L-arginine or N((1))-nitro-L-arginine-methyl ester (L-NAME). In all groups, cold increased catalase mRNA, protein expression and activity from day 3 to day 45 of acclimation. However, cold-acclimation increased GSH-Px mRNA content on day 3, protein content from day 1 to day 45 and activity no sooner than on day 45. At room temperature, 21- and 45-day L-arginine and L-NAME supplementation increased catalase and GSH-Px mRNA expression, but decreased their activity. Concomitantly, catalase protein content decreased, while GSH-Px protein expression increased compared to control. Data show that cold is dominant stimulus that determines IBAT catalase and GSH-Px responses, by setting tissue metabolic state. In thermogenic-active tissue, catalase is regulated on transcriptional level, while GSH-Px is regulated post-translationally. On the other side, it was shown that *NO affects catalase and GSH-Px only in thermogenic-inactive tissue. (C) 2011 Elsevier Ltd. All rights reserved.",
journal = "Journal of Thermal Biology",
title = "Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures",
number = "5",
volume = "36",
pages = "276",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1281"
}

Otašević, V., Buzadžić, B. J., Korac, A. B., Stančić, A., Janković, A., Vučetić, M.,& Korać, B.. (2011). Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures. in Journal of Thermal Biology, 36(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1281

Otašević V, Buzadžić BJ, Korac AB, Stančić A, Janković A, Vučetić M, Korać B. Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures. in Journal of Thermal Biology. 2011;36(5):null-276.
https://hdl.handle.net/21.15107/rcub_ibiss_1281 .

Otašević, Vesna, Buzadžić, Biljana J., Korac, Aleksandra B, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Korać, Bato, "Effects of L-arginine and L-NAME supplementation on mRNA, protein expression and activity of catalase and glutathione peroxidase in brown adipose tissue of rats acclimated to different temperatures" in Journal of Thermal Biology, 36, no. 5 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1281 .

TY  - JOUR
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Buzadžić, Biljana J.
AU  - Korać, Bato
AU  - Korac, Aleksandra B
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1324
AB  - The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis of endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy and also, to investigate the possible mechanisms underlying ECs death. In order to induce hyperinsulinaemia, adult male rats of Wistar strain were treated with high dose of insulin (4 U/kg, intraperitonely) for one or three days. Examinations at ultrastructural level showed apoptotic changes of ECs, allowing us to point out that changes mainly but not exclusively, occur in nuclei. Besides different stages of condensation and alterations of the chromatin, nuclear fragmentation was also observed. Higher number of ECs apoptotic nuclei in the BAT of hyperinsulinaemic rats was also confirmed by propidium iodide staining. Immunohistochemical localization of tumor necrosis factor-alpha (TNF-alpha) revealed increased expression in ECs of BAT of hyperinsulinaemic animals, indicating its possible role in insulin-induced apoptotic changes. These results suggest that BAT atrophy in hyperinsulinaemia is a result of endothelial and adipocyte apoptosis combined, rather than any of functional components alone.
T2  - European Journal of Histochemistry
T1  - Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha
IS  - 4
VL  - 55
EP  - 193
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1324
ER  - 

@article{
author = "Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Buzadžić, Biljana J. and Korać, Bato and Korac, Aleksandra B",
year = "2011",
abstract = "The aim of the present study was to investigate whether hyperinsulinaemia, which frequently precedes insulin resistance syndrome (obesity, diabetes), induces apoptosis of endothelial cells (ECs) in brown adipose tissue (BAT) and causes BAT atrophy and also, to investigate the possible mechanisms underlying ECs death. In order to induce hyperinsulinaemia, adult male rats of Wistar strain were treated with high dose of insulin (4 U/kg, intraperitonely) for one or three days. Examinations at ultrastructural level showed apoptotic changes of ECs, allowing us to point out that changes mainly but not exclusively, occur in nuclei. Besides different stages of condensation and alterations of the chromatin, nuclear fragmentation was also observed. Higher number of ECs apoptotic nuclei in the BAT of hyperinsulinaemic rats was also confirmed by propidium iodide staining. Immunohistochemical localization of tumor necrosis factor-alpha (TNF-alpha) revealed increased expression in ECs of BAT of hyperinsulinaemic animals, indicating its possible role in insulin-induced apoptotic changes. These results suggest that BAT atrophy in hyperinsulinaemia is a result of endothelial and adipocyte apoptosis combined, rather than any of functional components alone.",
journal = "European Journal of Histochemistry",
title = "Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha",
number = "4",
volume = "55",
pages = "193",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1324"
}

Markelić, M. B., Velicković, K. D., Golić, I., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Buzadžić, B. J., Korać, B.,& Korac, A. B.. (2011). Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha. in European Journal of Histochemistry, 55(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1324

Markelić MB, Velicković KD, Golić I, Otašević V, Stančić A, Janković A, Vučetić M, Buzadžić BJ, Korać B, Korac AB. Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha. in European Journal of Histochemistry. 2011;55(4):null-193.
https://hdl.handle.net/21.15107/rcub_ibiss_1324 .

Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Buzadžić, Biljana J., Korać, Bato, Korac, Aleksandra B, "Endothelial cell apoptosis in brown adipose tissue of rats induced by hyperinsulinaemia: the possible role of TNF-alpha" in European Journal of Histochemistry, 55, no. 4 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1324 .

TY  - CONF
AU  - Markelić, Milica B
AU  - Velicković, Ksenija D
AU  - Golić, Igor
AU  - Otašević, Vesna
AU  - Stančić, Ana
AU  - Janković, Aleksandra
AU  - Vučetić, Milica
AU  - Korać, Bato
AU  - Buzadžić, Biljana J.
AU  - Korac, Aleksandra B
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1314
C3  - Journal of Vascular Research
T1  - Insulin-induced microcirculation Remodelling in the rat brown adipose tissue
IS  - null
VL  - 48
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1314
ER  - 

@conference{
author = "Markelić, Milica B and Velicković, Ksenija D and Golić, Igor and Otašević, Vesna and Stančić, Ana and Janković, Aleksandra and Vučetić, Milica and Korać, Bato and Buzadžić, Biljana J. and Korac, Aleksandra B",
year = "2011",
journal = "Journal of Vascular Research",
title = "Insulin-induced microcirculation Remodelling in the rat brown adipose tissue",
number = "null",
volume = "48",
pages = "135",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1314"
}

Markelić, M. B., Velicković, K. D., Golić, I., Otašević, V., Stančić, A., Janković, A., Vučetić, M., Korać, B., Buzadžić, B. J.,& Korac, A. B.. (2011). Insulin-induced microcirculation Remodelling in the rat brown adipose tissue. in Journal of Vascular Research, 48(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1314

Markelić MB, Velicković KD, Golić I, Otašević V, Stančić A, Janković A, Vučetić M, Korać B, Buzadžić BJ, Korac AB. Insulin-induced microcirculation Remodelling in the rat brown adipose tissue. in Journal of Vascular Research. 2011;48(null):null-135.
https://hdl.handle.net/21.15107/rcub_ibiss_1314 .

Markelić, Milica B, Velicković, Ksenija D, Golić, Igor, Otašević, Vesna, Stančić, Ana, Janković, Aleksandra, Vučetić, Milica, Korać, Bato, Buzadžić, Biljana J., Korac, Aleksandra B, "Insulin-induced microcirculation Remodelling in the rat brown adipose tissue" in Journal of Vascular Research, 48, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1314 .