TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Nešović, Milica
AU  - Ćirić, Ivanka
AU  - Tešić, Živoslav
AU  - Pezo, Lato
AU  - Tosti, Tomislav
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Lončar, Biljana
AU  - Meland, Mekjell
PY  - 2022
UR  - https://www.mdpi.com/2311-7524/8/9/765
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5130
AB  - Raspberries are considered valuable fruits due to their high levels of nutrients and phytochemicals, which have many beneficial effects on humans. As many external factors affect the composition of these fruits (the type of cultivation, soil characteristics, ripeness, storage time and post-harvest technologies, cultivar/genotype, and climatic conditions), the goal of this study was to analyze different raspberry cultivars grown in Norway. Considering that Norway is a country with specific climatic conditions, as well as has a limited period of fruit vegetation, another important goal of this study was also to compare raspberries from different Norwegian areas, as well as different grown cultivars. Modern analytical techniques, such as high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPEAC-PAD), ultra-high-performance liquid chromatography with diode array detector coupled to triple quadrupole mass spectrometry (UHPLC-DAD MS/MS), and inductively coupled plasma–optical emission spectrometry (ICP-OES), provided a detailed examination of the raspberry extract samples. Based on their high levels of minerals (especially N, P, and K), organic acids (predominantly citric and malic acids), sugars (glucose, fructose, sucrose, and galactose), and polyphenols (ellagic acid, syringic acid, quercetin, and rutin), Norwegian raspberries could be considered fruits with increased health-beneficial compounds. The chemical composition of the studied cultivars depended on the locality of growth.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions
IS  - 9
VL  - 8
DO  - 10.3390/horticulturae8090765
SP  - 765
ER  - 

@article{
author = "Fotirić Akšić, Milica and Nešović, Milica and Ćirić, Ivanka and Tešić, Živoslav and Pezo, Lato and Tosti, Tomislav and Gašić, Uroš and Dojčinović, Biljana and Lončar, Biljana and Meland, Mekjell",
year = "2022",
abstract = "Raspberries are considered valuable fruits due to their high levels of nutrients and phytochemicals, which have many beneficial effects on humans. As many external factors affect the composition of these fruits (the type of cultivation, soil characteristics, ripeness, storage time and post-harvest technologies, cultivar/genotype, and climatic conditions), the goal of this study was to analyze different raspberry cultivars grown in Norway. Considering that Norway is a country with specific climatic conditions, as well as has a limited period of fruit vegetation, another important goal of this study was also to compare raspberries from different Norwegian areas, as well as different grown cultivars. Modern analytical techniques, such as high-performance anion-exchange liquid chromatography with pulsed amperometric detection (HPEAC-PAD), ultra-high-performance liquid chromatography with diode array detector coupled to triple quadrupole mass spectrometry (UHPLC-DAD MS/MS), and inductively coupled plasma–optical emission spectrometry (ICP-OES), provided a detailed examination of the raspberry extract samples. Based on their high levels of minerals (especially N, P, and K), organic acids (predominantly citric and malic acids), sugars (glucose, fructose, sucrose, and galactose), and polyphenols (ellagic acid, syringic acid, quercetin, and rutin), Norwegian raspberries could be considered fruits with increased health-beneficial compounds. The chemical composition of the studied cultivars depended on the locality of growth.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions",
number = "9",
volume = "8",
doi = "10.3390/horticulturae8090765",
pages = "765"
}

Fotirić Akšić, M., Nešović, M., Ćirić, I., Tešić, Ž., Pezo, L., Tosti, T., Gašić, U., Dojčinović, B., Lončar, B.,& Meland, M.. (2022). Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions. in Horticulturae
Basel: MDPI., 8(9), 765.
https://doi.org/10.3390/horticulturae8090765

Fotirić Akšić M, Nešović M, Ćirić I, Tešić Ž, Pezo L, Tosti T, Gašić U, Dojčinović B, Lončar B, Meland M. Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions. in Horticulturae. 2022;8(9):765.
doi:10.3390/horticulturae8090765 .

Fotirić Akšić, Milica, Nešović, Milica, Ćirić, Ivanka, Tešić, Živoslav, Pezo, Lato, Tosti, Tomislav, Gašić, Uroš, Dojčinović, Biljana, Lončar, Biljana, Meland, Mekjell, "Chemical Fruit Profiles of Different Raspberry Cultivars Grown in Specific Norwegian Agroclimatic Conditions" in Horticulturae, 8, no. 9 (2022):765,
https://doi.org/10.3390/horticulturae8090765 . .

TY  - JOUR
AU  - Fotirić Akšić, Milica
AU  - Nešović, Milica
AU  - Ćirić, Ivanka
AU  - Tešić, Živoslav
AU  - Pezo, Lato
AU  - Tosti, Tomislav
AU  - Gašić, Uroš
AU  - Dojčinović, Biljana
AU  - Lončar, Biljana
AU  - Meland, Mekjell
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.941487/full
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5081
AB  - Using modern analytical techniques, a comprehensive study of the chemical composition of fruits from apple cultivars grown in Western Norway during 2019 and 2020 was done. Metals, sugars, organic acids, antioxidant tests, and polyphenol content have been observed. In all investigated samples, the most dominant sugars were glucose, fructose, and sucrose. Among 11 tested organic acids, the dominant was malic acid, followed by citric and maleic acid. The most common metal was potassium, followed by magnesium and zinc. The quantification of polyphenols showed that among the 11 quantified polyphenols, chlorogenic acid, quercetin 3- O- rhamnoside, quercetin 3 -O -glucoside, quercetin, and phlorizin were the most abundant. A detailed study of the polyphenolic profile of nine investigated apple samples provided 30 identified polyphenolic compounds from the class of hydroxybenzoic and hydroxycinnamic acids, flavonoids, and dihydrochalcones. In addition to the identified 3- O -caffeoylquinic acid, its two isomers of 5- O -caffeoylquinic acid and three esters were also found. Present polyphenols of the tested apples provided significant data on the quality of Norwegian apples, and they contribute to the distinguishing of these apple samples.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars
VL  - 9
DO  - 10.3389/fnut.2022.941487
SP  - 941487
ER  - 

@article{
author = "Fotirić Akšić, Milica and Nešović, Milica and Ćirić, Ivanka and Tešić, Živoslav and Pezo, Lato and Tosti, Tomislav and Gašić, Uroš and Dojčinović, Biljana and Lončar, Biljana and Meland, Mekjell",
year = "2022",
abstract = "Using modern analytical techniques, a comprehensive study of the chemical composition of fruits from apple cultivars grown in Western Norway during 2019 and 2020 was done. Metals, sugars, organic acids, antioxidant tests, and polyphenol content have been observed. In all investigated samples, the most dominant sugars were glucose, fructose, and sucrose. Among 11 tested organic acids, the dominant was malic acid, followed by citric and maleic acid. The most common metal was potassium, followed by magnesium and zinc. The quantification of polyphenols showed that among the 11 quantified polyphenols, chlorogenic acid, quercetin 3- O- rhamnoside, quercetin 3 -O -glucoside, quercetin, and phlorizin were the most abundant. A detailed study of the polyphenolic profile of nine investigated apple samples provided 30 identified polyphenolic compounds from the class of hydroxybenzoic and hydroxycinnamic acids, flavonoids, and dihydrochalcones. In addition to the identified 3- O -caffeoylquinic acid, its two isomers of 5- O -caffeoylquinic acid and three esters were also found. Present polyphenols of the tested apples provided significant data on the quality of Norwegian apples, and they contribute to the distinguishing of these apple samples.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars",
volume = "9",
doi = "10.3389/fnut.2022.941487",
pages = "941487"
}

Fotirić Akšić, M., Nešović, M., Ćirić, I., Tešić, Ž., Pezo, L., Tosti, T., Gašić, U., Dojčinović, B., Lončar, B.,& Meland, M.. (2022). Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 941487.
https://doi.org/10.3389/fnut.2022.941487

Fotirić Akšić M, Nešović M, Ćirić I, Tešić Ž, Pezo L, Tosti T, Gašić U, Dojčinović B, Lončar B, Meland M. Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars. in Frontiers in Nutrition. 2022;9:941487.
doi:10.3389/fnut.2022.941487 .

Fotirić Akšić, Milica, Nešović, Milica, Ćirić, Ivanka, Tešić, Živoslav, Pezo, Lato, Tosti, Tomislav, Gašić, Uroš, Dojčinović, Biljana, Lončar, Biljana, Meland, Mekjell, "Polyphenolics and Chemical Profiles of Domestic Norwegian Apple (Malus × domestica Borkh.) Cultivars" in Frontiers in Nutrition, 9 (2022):941487,
https://doi.org/10.3389/fnut.2022.941487 . .

TY  - JOUR
AU  - Nedić, Nebojša
AU  - Nešović, Milica
AU  - Radišić, Predrag
AU  - Gašić, Uroš
AU  - Baošić, Rada
AU  - Joksimović, Kristina
AU  - Pezo, Lato
AU  - Tešić, Živoslav
AU  - Vovk, Irena
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnut.2022.941463/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9263698
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5080
AB  - This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Nutrition
T1  - Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.
VL  - 9
DO  - 10.3389/fnut.2022.941463
SP  - 941463
ER  - 

@article{
author = "Nedić, Nebojša and Nešović, Milica and Radišić, Predrag and Gašić, Uroš and Baošić, Rada and Joksimović, Kristina and Pezo, Lato and Tešić, Živoslav and Vovk, Irena",
year = "2022",
abstract = "This study presents a detailed characterization of 27 honey samples from the Tara Mountain region in Serbia using different comprehensive techniques and methods. The types of the honey samples were defined as monofloral (4 samples), honeydew (5 samples) and polyfloral (18 samples) honey based on determined polyphenol content, antioxidant activity, electrical conductivity and melissopalynological analyses. Physicochemical parameters such as pH (4.13-4.94), diastase activity (24.20-41.70 DN), acidity (14.60-29.70 meq/kg), content of 5-(hydroxymethyl)furfural (in range below 5, up to 16.90 mg/kg), sucrose (0.20-3.90 g/100 g), and moisture content (15.01-19.23%) confirmed the required quality of the honey samples. Sensory analysis revealed honey characteristics favorable to consumers. Analyses of 19 phenolic compounds using ultra-high-performance liquid chromatography with a diode-array detection and triple quadrupole mass spectrometry (UHPLC-DAD-MS/MS) revealed six phenolic acids and 13 other compounds from the group of flavonoids and their glycosides. In all the samples the highest content was determined for p-coumaric acid, followed by caffeic acid and pinocembrin. Besides total phenolic content and radical scavenging activity, antimicrobial activity was also examined. Most honey samples showed bactericidal activity against Staphylococcus aureus and bacteriostatic activity against Escherichia coli, while none of the honey samples inhibited the growth of Candida albicans. Chemometric analyses were applied for an in-depth study of the results to further evaluate the characteristics of the honey samples studied. Principal component analysis (PCA) was used for assessing the differences in physicochemical parameters, polyphenols content and antioxidant capacity between honey samples. The unrooted cluster tree was used to group the samples based on the melissopalynological analyses.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Nutrition",
title = "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.",
volume = "9",
doi = "10.3389/fnut.2022.941463",
pages = "941463"
}

Nedić, N., Nešović, M., Radišić, P., Gašić, U., Baošić, R., Joksimović, K., Pezo, L., Tešić, Ž.,& Vovk, I.. (2022). Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition
Lausanne: Frontiers Media S.A.., 9, 941463.
https://doi.org/10.3389/fnut.2022.941463

Nedić N, Nešović M, Radišić P, Gašić U, Baošić R, Joksimović K, Pezo L, Tešić Ž, Vovk I. Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia.. in Frontiers in Nutrition. 2022;9:941463.
doi:10.3389/fnut.2022.941463 .

Nedić, Nebojša, Nešović, Milica, Radišić, Predrag, Gašić, Uroš, Baošić, Rada, Joksimović, Kristina, Pezo, Lato, Tešić, Živoslav, Vovk, Irena, "Polyphenolic and Chemical Profiles of Honey From the Tara Mountain in Serbia." in Frontiers in Nutrition, 9 (2022):941463,
https://doi.org/10.3389/fnut.2022.941463 . .

TY  - JOUR
AU  - Bugeja Douglas, Adrian
AU  - Nešović, Milica
AU  - Šikoparija, Branko
AU  - Radišić, Predrag
AU  - Tosti, Tomislav
AU  - Trifković, Jelena
AU  - Russi, Luigi
AU  - Attard, Everaldo
AU  - Tešić, Živoslav
AU  - Gašić, Uroš
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5665
AB  - Malta, a country renowned for its honey, has not been extensively mentioned in studies based on honey. In addition to many parameters, the collection period affects honey quality, precisely due to the different floral composition that exists during a certain season. Therefore, the significance of this study refers to the provision of data on honey from Malta collected during the autumn, spring, and summer seasons. Melissopalynological analysis, determination of physicochemical parameters, and the use of analytical chromatographic methods enabled detailed analysis of this honey. Principal component analysis (PCA) provided the differentiation of Maltese honey depending on the harvest season. Lotus pollen, followed by Eucalyptus, predominated in all honey samples. Characteristic compounds for summer honey were pinocembrin, galangin, kaempferol, chrysin, p-hydroxybenzoic acid, vanillic acid and maltotriose, while quercetin 3-O-galactoside, ferulic acid, ellagic acid, protocatechuic acid, luteolin 7-O-glucoside and melibiose were specific for autumn honey. A higher amount of p-coumaric acid, genistein, catechin, as well as the content of many sugars were found in spring samples. To the best of our knowledge, this is the first scientific work dealing with a detailed chemical analysis of Maltese honey.
PB  - Belgrade: Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons
IS  - 9
VL  - 87
DO  - 10.2298/JSC211214033B
SP  - 983
EP  - 995
ER  - 

@article{
author = "Bugeja Douglas, Adrian and Nešović, Milica and Šikoparija, Branko and Radišić, Predrag and Tosti, Tomislav and Trifković, Jelena and Russi, Luigi and Attard, Everaldo and Tešić, Živoslav and Gašić, Uroš",
year = "2022",
abstract = "Malta, a country renowned for its honey, has not been extensively mentioned in studies based on honey. In addition to many parameters, the collection period affects honey quality, precisely due to the different floral composition that exists during a certain season. Therefore, the significance of this study refers to the provision of data on honey from Malta collected during the autumn, spring, and summer seasons. Melissopalynological analysis, determination of physicochemical parameters, and the use of analytical chromatographic methods enabled detailed analysis of this honey. Principal component analysis (PCA) provided the differentiation of Maltese honey depending on the harvest season. Lotus pollen, followed by Eucalyptus, predominated in all honey samples. Characteristic compounds for summer honey were pinocembrin, galangin, kaempferol, chrysin, p-hydroxybenzoic acid, vanillic acid and maltotriose, while quercetin 3-O-galactoside, ferulic acid, ellagic acid, protocatechuic acid, luteolin 7-O-glucoside and melibiose were specific for autumn honey. A higher amount of p-coumaric acid, genistein, catechin, as well as the content of many sugars were found in spring samples. To the best of our knowledge, this is the first scientific work dealing with a detailed chemical analysis of Maltese honey.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons",
number = "9",
volume = "87",
doi = "10.2298/JSC211214033B",
pages = "983-995"
}

Bugeja Douglas, A., Nešović, M., Šikoparija, B., Radišić, P., Tosti, T., Trifković, J., Russi, L., Attard, E., Tešić, Ž.,& Gašić, U.. (2022). Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons. in Journal of the Serbian Chemical Society
Belgrade: Serbian Chemical Society., 87(9), 983-995.
https://doi.org/10.2298/JSC211214033B

Bugeja Douglas A, Nešović M, Šikoparija B, Radišić P, Tosti T, Trifković J, Russi L, Attard E, Tešić Ž, Gašić U. Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons. in Journal of the Serbian Chemical Society. 2022;87(9):983-995.
doi:10.2298/JSC211214033B .

Bugeja Douglas, Adrian, Nešović, Milica, Šikoparija, Branko, Radišić, Predrag, Tosti, Tomislav, Trifković, Jelena, Russi, Luigi, Attard, Everaldo, Tešić, Živoslav, Gašić, Uroš, "Melissopalynology analysis, determination of physicochemical parameters, sugars and phenolics in Maltese honey collected in different seasons" in Journal of the Serbian Chemical Society, 87, no. 9 (2022):983-995,
https://doi.org/10.2298/JSC211214033B . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Nedić, Nebojša
AU  - Sredojević, Milica
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4314
AB  - The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.
PB  - Cambridge: Royal Society of Chemistry
T2  - RSC Advances
T1  - Distribution of polyphenolic and sugar compounds in different buckwheat plant parts
IS  - 42
VL  - 11
DO  - 10.1039/D1RA04250E
SP  - 25816
EP  - 25829
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Horvacki, Nikola and Nedić, Nebojša and Sredojević, Milica and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2021",
abstract = "The aim of this study was to provide information on the phenolic and sugar profiles of different parts of the buckwheat plant, which can define that buckwheat is a functional food, with a high nutritional value and very useful for human health. Therefore, the extracts of buckwheat leaf, stem, and flower, as well as buckwheat grain were analysed for the content of polyphenol and antioxidant tests. The identification of a notable number of phenolic compounds and quantification of sugars in different parts of buckwheat indicates that buckwheat is a highly valuable plant. A total of 60 phenolic compounds were identified (18 cinnamic acid derivatives, 14 flavonols, 13 flavan-3-ols (including proanthocyanidins), 10 hydroxybenzoic acid derivatives, and 5 flavones) using ultra-high-performance liquid chromatography (UHPLC), coupled with a hybrid mass spectrometer which combines the Linear Trap Quadrupole (LTQ) and OrbiTrap mass analyzer. The highest number of phenolic compounds was found in the analysed buckwheat flower sample, and then in the leaf, followed by the grain and the stem. In addition, the sugar profile of buckwheat leaf, stem, flower and grain, as well as the buckwheat pollen and the nectar was analysed. Hence, 16 sugars and 5 sugar alcohols were detected by the high-performance anion exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD). Sucrose was found in a significant amount with the highest content in buckwheat leaf. Trisaccharides had similar accumulation in the sample extracts, while disaccharides dominated in buckwheat leaf, followed by nectar and pollen. The sugar alcohols showed the highest content in buckwheat grain, where erythritol was predominant. The obtained results show that buckwheat is very rich in phenolic compounds and sugars. In addition to grain, the other parts of the buckwheat plant can be used as a very good source of different classes of phenolic compounds. This study provides useful information on the distribution of phytochemicals in different parts of the buckwheat plant, which contribute to the maintaining of the status of buckwheat as a functional food.",
publisher = "Cambridge: Royal Society of Chemistry",
journal = "RSC Advances",
title = "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts",
number = "42",
volume = "11",
doi = "10.1039/D1RA04250E",
pages = "25816-25829"
}

Nešović, M., Gašić, U., Tosti, T., Horvacki, N., Nedić, N., Sredojević, M., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2021). Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances
Cambridge: Royal Society of Chemistry., 11(42), 25816-25829.
https://doi.org/10.1039/D1RA04250E

Nešović M, Gašić U, Tosti T, Horvacki N, Nedić N, Sredojević M, Blagojević S, Ignjatović L, Tešić Ž. Distribution of polyphenolic and sugar compounds in different buckwheat plant parts. in RSC Advances. 2021;11(42):25816-25829.
doi:10.1039/D1RA04250E .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Horvacki, Nikola, Nedić, Nebojša, Sredojević, Milica, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Distribution of polyphenolic and sugar compounds in different buckwheat plant parts" in RSC Advances, 11, no. 42 (2021):25816-25829,
https://doi.org/10.1039/D1RA04250E . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Horvacki, Nikola
AU  - Šikoparija, Branko
AU  - Nedić, Nebojša
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4058
AB  - A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.
PB  - Royal Society of Chemistry
T2  - Royal Society Open Science
T1  - Polyphenolic profile of buckwheat honey, nectar and pollen
IS  - 12
VL  - 7
DO  - 10.1098/rsos.201576
SP  - 201576
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Horvacki, Nikola and Šikoparija, Branko and Nedić, Nebojša and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2020",
abstract = "A focus of research in recent years is the comparison of honey as the final product of bees with pollen and nectar of the plant from which the honey originates, as the main food source for bees. Buckwheat honey is recognized as a nutritionally valuable product, which provides a scientifically proven health benefit and is confirmed as a functional food. The quality of this type of honey is attributed to high levels of phytochemicals in buckwheat. The purpose of this study was the examination of similarity between buckwheat honey and buckwheat nectar and pollen, as well as simultaneous investigation of their chemical profiles and the origin of the honey. The phenolic profile of buckwheat pollen showed a lower number of flavonoids and phenolic acids than those of nectar and honey samples, but confirmed the presence of the most characteristic polyphenols derived from the buckwheat plant. The notable difference was found to be the presence of (epi)catechin units, its galloylated derivatives and procyanidin dimers, which were not present in honey. Honey polyphenols displayed a pronounced correlation with those of nectar, but not with those of pollen. Finally, by comparing the polyphenolic profiles of honey, nectar and pollen sharing the same geographical origin, new data could be provided for a potential assessment of the botanical origin of buckwheat honey.",
publisher = "Royal Society of Chemistry",
journal = "Royal Society Open Science",
title = "Polyphenolic profile of buckwheat honey, nectar and pollen",
number = "12",
volume = "7",
doi = "10.1098/rsos.201576",
pages = "201576"
}

Nešović, M., Gašić, U., Tosti, T., Horvacki, N., Šikoparija, B., Nedić, N., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2020). Polyphenolic profile of buckwheat honey, nectar and pollen. in Royal Society Open Science
Royal Society of Chemistry., 7(12), 201576.
https://doi.org/10.1098/rsos.201576

Nešović M, Gašić U, Tosti T, Horvacki N, Šikoparija B, Nedić N, Blagojević S, Ignjatović L, Tešić Ž. Polyphenolic profile of buckwheat honey, nectar and pollen. in Royal Society Open Science. 2020;7(12):201576.
doi:10.1098/rsos.201576 .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Horvacki, Nikola, Šikoparija, Branko, Nedić, Nebojša, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Polyphenolic profile of buckwheat honey, nectar and pollen" in Royal Society Open Science, 7, no. 12 (2020):201576,
https://doi.org/10.1098/rsos.201576 . .

TY  - JOUR
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tosti, Tomislav
AU  - Trifković, Jelena
AU  - Baošić, Rada
AU  - Blagojević, Stevan
AU  - Ignjatović, Ljubiša
AU  - Tešić, Živoslav
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3600
AB  - The research subject of this paper was a detail physicochemical analysis of 28 honey samples from the northern part of Montenegro. The honey from Montenegro has not been previously studied in such detail. Differentiation between samples, such as honeydew honey and polyfloral honey, was based on electrical conductivity, which was higher than 0.8 mS cm-1 for honeydew honey, as was expected. Other investigated physicochemical parameters (water content, free acids, diastase activity, hydroxymethylfurfural (HMF) content and sugar content) have shown great similarity for all honey samples. The main interest of this study was the identification and quantification of phenolic compounds using ultra-high performance liquid chromatography (UHPLC) with mass spectrometry detection. The results show that honey samples are very rich in phenolic compounds, especially quercetin. Among the 31 quantified phenolic compounds, the most dominant were phenolic acids. The highlight was based on p-hydroxybenzoic acid, p-coumaric acid, caffeic acid and ferulic acid. Considering polyphenolic compounds and sugar content, a high nutritional value can be observed in all samples, with an emphasis on polyfloral honeys, as was confirmed with principal component analysis (PCA). In addition, all honey samples were tested for total phenolic content (TPC) and radical scavenging activity (RSA). The results indicate the higher antioxidant ability of honeys from Montenegro in comparison to some honey samples from other countries in the region.
T2  - RSC Advances
T1  - Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro
IS  - 5
VL  - 10
DO  - 10.1039/c9ra08783d
SP  - 2462
EP  - 2471
ER  - 

@article{
author = "Nešović, Milica and Gašić, Uroš and Tosti, Tomislav and Trifković, Jelena and Baošić, Rada and Blagojević, Stevan and Ignjatović, Ljubiša and Tešić, Živoslav",
year = "2020",
abstract = "The research subject of this paper was a detail physicochemical analysis of 28 honey samples from the northern part of Montenegro. The honey from Montenegro has not been previously studied in such detail. Differentiation between samples, such as honeydew honey and polyfloral honey, was based on electrical conductivity, which was higher than 0.8 mS cm-1 for honeydew honey, as was expected. Other investigated physicochemical parameters (water content, free acids, diastase activity, hydroxymethylfurfural (HMF) content and sugar content) have shown great similarity for all honey samples. The main interest of this study was the identification and quantification of phenolic compounds using ultra-high performance liquid chromatography (UHPLC) with mass spectrometry detection. The results show that honey samples are very rich in phenolic compounds, especially quercetin. Among the 31 quantified phenolic compounds, the most dominant were phenolic acids. The highlight was based on p-hydroxybenzoic acid, p-coumaric acid, caffeic acid and ferulic acid. Considering polyphenolic compounds and sugar content, a high nutritional value can be observed in all samples, with an emphasis on polyfloral honeys, as was confirmed with principal component analysis (PCA). In addition, all honey samples were tested for total phenolic content (TPC) and radical scavenging activity (RSA). The results indicate the higher antioxidant ability of honeys from Montenegro in comparison to some honey samples from other countries in the region.",
journal = "RSC Advances",
title = "Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro",
number = "5",
volume = "10",
doi = "10.1039/c9ra08783d",
pages = "2462-2471"
}

Nešović, M., Gašić, U., Tosti, T., Trifković, J., Baošić, R., Blagojević, S., Ignjatović, L.,& Tešić, Ž.. (2020). Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro. in RSC Advances, 10(5), 2462-2471.
https://doi.org/10.1039/c9ra08783d

Nešović M, Gašić U, Tosti T, Trifković J, Baošić R, Blagojević S, Ignjatović L, Tešić Ž. Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro. in RSC Advances. 2020;10(5):2462-2471.
doi:10.1039/c9ra08783d .

Nešović, Milica, Gašić, Uroš, Tosti, Tomislav, Trifković, Jelena, Baošić, Rada, Blagojević, Stevan, Ignjatović, Ljubiša, Tešić, Živoslav, "Physicochemical analysis and phenolic profile of polyfloral and honeydew honey from Montenegro" in RSC Advances, 10, no. 5 (2020):2462-2471,
https://doi.org/10.1039/c9ra08783d . .

TY  - CONF
AU  - Nešović, Milica
AU  - Gašić, Uroš
AU  - Tešić, Živoslav
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5907
AB  - Introduction: Honey is a high energetic sweet food composed, among other components, of
bioactive compounds that attribute therapeutic properties of this product. As antioxidants,
polyphenols are recognized as the major constituents in honey responsible for its health
promoting properties. Bees produce a floral honey from the collected nectar of flowers, and
honeydew honey from collected excrement of insects or plants. Considering that Montenegro
is a country with traditional beekeeping, this is the first time to publish the results of its
honeydew and polyfloral honey samples.
Experimental: Analyzed honey samples, honeydew and polyfloral, were collected from three
locations in norther part of Montenegro, Berane, Bijelo Polje and Pljevlja. Polyphenol profile
of six honey samples was determinated by identifying polyphenols on UHPLC-LTQ OrbiTrap
MS, and by their quantifications on UHPLC-DAD MS/MS.
Results: Of the 32 identified polyphenol compounds, 24 were present in all samples. Appearing
the other eight polyphenol compounds has no constant diversity between the samples. The
similarity between them was confirmed by their quantifications. The highest proportion in total
polyphenols has a quercetin with a mean value of 3.82 mg/kg, followed by p-hydrohybenzoic
acid and p-coumaric acid. Slightly higher content of chrysin, pinocembrin, p-protocatechuic
and vanillic acid can be noticed. The quantifications of the target compounds include 33
polyphenols, and only 16 of them were previously identified. This indicates the occurrence of
certain dissimilarities between two used techniques, which are the consequences of similar
spectra of certain polyphenols and their derivatives.
Conclusion: This is the detailed study of polyphenols in Montenegrin honeys. Based on the
obtained results, it can be seen that the investigated honey samples are very rich in polyphenols,
with no significant differences between honeydew and polyfloral samples. Considering small
areas that cover these three regions of norther part of Montenegro, it can be assumed that small
diversity in plant species causes overlapping in honeybee’s pastures and similarity of samples
PB  - Novi Sad: Faculty of Technology
C3  - Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia
T1  - Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5907
ER  - 

@conference{
author = "Nešović, Milica and Gašić, Uroš and Tešić, Živoslav",
year = "2019",
abstract = "Introduction: Honey is a high energetic sweet food composed, among other components, of
bioactive compounds that attribute therapeutic properties of this product. As antioxidants,
polyphenols are recognized as the major constituents in honey responsible for its health
promoting properties. Bees produce a floral honey from the collected nectar of flowers, and
honeydew honey from collected excrement of insects or plants. Considering that Montenegro
is a country with traditional beekeeping, this is the first time to publish the results of its
honeydew and polyfloral honey samples.
Experimental: Analyzed honey samples, honeydew and polyfloral, were collected from three
locations in norther part of Montenegro, Berane, Bijelo Polje and Pljevlja. Polyphenol profile
of six honey samples was determinated by identifying polyphenols on UHPLC-LTQ OrbiTrap
MS, and by their quantifications on UHPLC-DAD MS/MS.
Results: Of the 32 identified polyphenol compounds, 24 were present in all samples. Appearing
the other eight polyphenol compounds has no constant diversity between the samples. The
similarity between them was confirmed by their quantifications. The highest proportion in total
polyphenols has a quercetin with a mean value of 3.82 mg/kg, followed by p-hydrohybenzoic
acid and p-coumaric acid. Slightly higher content of chrysin, pinocembrin, p-protocatechuic
and vanillic acid can be noticed. The quantifications of the target compounds include 33
polyphenols, and only 16 of them were previously identified. This indicates the occurrence of
certain dissimilarities between two used techniques, which are the consequences of similar
spectra of certain polyphenols and their derivatives.
Conclusion: This is the detailed study of polyphenols in Montenegrin honeys. Based on the
obtained results, it can be seen that the investigated honey samples are very rich in polyphenols,
with no significant differences between honeydew and polyfloral samples. Considering small
areas that cover these three regions of norther part of Montenegro, it can be assumed that small
diversity in plant species causes overlapping in honeybee’s pastures and similarity of samples",
publisher = "Novi Sad: Faculty of Technology",
journal = "Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia",
title = "Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro",
pages = "39",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5907"
}

Nešović, M., Gašić, U.,& Tešić, Ž.. (2019). Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro. in Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia
Novi Sad: Faculty of Technology., 39.
https://hdl.handle.net/21.15107/rcub_ibiss_5907

Nešović M, Gašić U, Tešić Ž. Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro. in Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia. 2019;:39.
https://hdl.handle.net/21.15107/rcub_ibiss_5907 .

Nešović, Milica, Gašić, Uroš, Tešić, Živoslav, "Polyphenol profile of honeydew and polyfloral honey from northern part of Montenegro" in Book of abstracts: 1st International Conference on Advanced Production and Processing: 2019 Oct 10-11; Novi Sad, Serbia (2019):39,
https://hdl.handle.net/21.15107/rcub_ibiss_5907 .