TY  - JOUR
AU  - Zakić, Tamara
AU  - Kalezić, Anđelika
AU  - Drvendžija, Zorka
AU  - Udicki, Mirjana
AU  - Ivković Kapicl, Tatjana
AU  - Srdić Galić, Biljana
AU  - Korać, Aleksandra
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6528
AB  - The close cooperation between breast cancer and cancer-associated adipose tissue (CAAT) shapes the malignant phenotype, but the role of mitochondrial metabolic reprogramming and obesity in breast cancer remains undecided, especially in premenopausal women. Here, we examined mitochondrial metabolic dynamics in paired biopsies of malignant versus benign breast tumor tissue and CAAT in normal-weight and overweight/obese premenopausal women. Lower protein level of pyruvate dehydrogenase and citrate synthase in malignant tumor tissue indicated decreased carbon flux from glucose into the Krebs cycle, whereas the trend was just the opposite in malignant CAAT. Simultaneously, stimulated lipolysis in CAAT of obese women was followed by upregulated β-oxidation, as well as fatty acid synthesis enzymes in both tumor tissue and CAAT of women with malignant tumors, corroborating their physical association. Further, protein level of electron transport chain complexes was generally increased in tumor tissue and CAAT from women with malignant tumors, respective to obesity. Preserved mitochondrial structure in malignant tumor tissue was also observed. However, mitochondrial DNA copy number and protein levels of PGC-1α were dependent on both malignancy and obesity in tumor tissue and CAAT. In conclusion, metabolic cooperation between breast cancer and CAAT in premenopausal women involves obesity-related, synchronized changes in mitochondrial metabolism.
PB  - Basel: MDPI
T2  - Cells
T1  - Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue
IS  - 2
VL  - 13
DO  - 10.3390/cells13020155
SP  - 155
ER  - 

@article{
author = "Zakić, Tamara and Kalezić, Anđelika and Drvendžija, Zorka and Udicki, Mirjana and Ivković Kapicl, Tatjana and Srdić Galić, Biljana and Korać, Aleksandra and Janković, Aleksandra and Korać, Bato",
year = "2024",
abstract = "The close cooperation between breast cancer and cancer-associated adipose tissue (CAAT) shapes the malignant phenotype, but the role of mitochondrial metabolic reprogramming and obesity in breast cancer remains undecided, especially in premenopausal women. Here, we examined mitochondrial metabolic dynamics in paired biopsies of malignant versus benign breast tumor tissue and CAAT in normal-weight and overweight/obese premenopausal women. Lower protein level of pyruvate dehydrogenase and citrate synthase in malignant tumor tissue indicated decreased carbon flux from glucose into the Krebs cycle, whereas the trend was just the opposite in malignant CAAT. Simultaneously, stimulated lipolysis in CAAT of obese women was followed by upregulated β-oxidation, as well as fatty acid synthesis enzymes in both tumor tissue and CAAT of women with malignant tumors, corroborating their physical association. Further, protein level of electron transport chain complexes was generally increased in tumor tissue and CAAT from women with malignant tumors, respective to obesity. Preserved mitochondrial structure in malignant tumor tissue was also observed. However, mitochondrial DNA copy number and protein levels of PGC-1α were dependent on both malignancy and obesity in tumor tissue and CAAT. In conclusion, metabolic cooperation between breast cancer and CAAT in premenopausal women involves obesity-related, synchronized changes in mitochondrial metabolism.",
publisher = "Basel: MDPI",
journal = "Cells",
title = "Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue",
number = "2",
volume = "13",
doi = "10.3390/cells13020155",
pages = "155"
}

Zakić, T., Kalezić, A., Drvendžija, Z., Udicki, M., Ivković Kapicl, T., Srdić Galić, B., Korać, A., Janković, A.,& Korać, B.. (2024). Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue. in Cells
Basel: MDPI., 13(2), 155.
https://doi.org/10.3390/cells13020155

Zakić T, Kalezić A, Drvendžija Z, Udicki M, Ivković Kapicl T, Srdić Galić B, Korać A, Janković A, Korać B. Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue. in Cells. 2024;13(2):155.
doi:10.3390/cells13020155 .

Zakić, Tamara, Kalezić, Anđelika, Drvendžija, Zorka, Udicki, Mirjana, Ivković Kapicl, Tatjana, Srdić Galić, Biljana, Korać, Aleksandra, Janković, Aleksandra, Korać, Bato, "Breast Cancer: Mitochondria-Centered Metabolic Alterations in Tumor and Associated Adipose Tissue" in Cells, 13, no. 2 (2024):155,
https://doi.org/10.3390/cells13020155 . .

TY  - CONF
AU  - Kalezić, Anđelika
AU  - Udicki, Mirjana
AU  - Srdić Galić, Biljana
AU  - Korać, Aleksandra
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4681
AB  - Global trends of increasing caloric consumption and decreasing physical activity have given rise to metabolic diseases in epidemic proportions. As a chronic state of altered metabolic and energy homeostasis,obesity is associated with various chronic diseases, including cancer. Alongside obesity, breast cancer incidence is also on the rise,even among premenopausal women. Acting through systemic and local influences, obesity could be viewed as a potential driving force for breast cancer development; however, this relationship seems rather complex. Accumulating evidence indicates that obesity decreasesthe incidence of breast cancer but increases rates of mortality,metastasis and therapeutic resistance in premenopausal women. We aim to provide an in-depth insight into the molecular mechanisms underlying the intriguing relationship between breast cancer and obesity by focusing on cross-examinationof metabolic alterations in breast cancer and cancer-associated adipose tissue. Metabolic reprogramming will be discussed through the potential influences obesityexerts on major pathways in glucose, lipid, and mitochondrial metabolism in breast cancer and cancer-associated adipose tissue. An overview of the wide-ranging implications of context-dependent metabolic reprogramming for personalized diagnostic and therapeutic approaches will be given.
PB  - Belgrade: Serbian Nutrition Society
C3  - 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia
T1  - Molecular basis of obesity and cancer
SP  - 55
EP  - 55
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4681
ER  - 

@conference{
author = "Kalezić, Anđelika and Udicki, Mirjana and Srdić Galić, Biljana and Korać, Aleksandra and Janković, Aleksandra and Korać, Bato",
year = "2021",
abstract = "Global trends of increasing caloric consumption and decreasing physical activity have given rise to metabolic diseases in epidemic proportions. As a chronic state of altered metabolic and energy homeostasis,obesity is associated with various chronic diseases, including cancer. Alongside obesity, breast cancer incidence is also on the rise,even among premenopausal women. Acting through systemic and local influences, obesity could be viewed as a potential driving force for breast cancer development; however, this relationship seems rather complex. Accumulating evidence indicates that obesity decreasesthe incidence of breast cancer but increases rates of mortality,metastasis and therapeutic resistance in premenopausal women. We aim to provide an in-depth insight into the molecular mechanisms underlying the intriguing relationship between breast cancer and obesity by focusing on cross-examinationof metabolic alterations in breast cancer and cancer-associated adipose tissue. Metabolic reprogramming will be discussed through the potential influences obesityexerts on major pathways in glucose, lipid, and mitochondrial metabolism in breast cancer and cancer-associated adipose tissue. An overview of the wide-ranging implications of context-dependent metabolic reprogramming for personalized diagnostic and therapeutic approaches will be given.",
publisher = "Belgrade: Serbian Nutrition Society",
journal = "14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia",
title = "Molecular basis of obesity and cancer",
pages = "55-55",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4681"
}

Kalezić, A., Udicki, M., Srdić Galić, B., Korać, A., Janković, A.,& Korać, B.. (2021). Molecular basis of obesity and cancer. in 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia
Belgrade: Serbian Nutrition Society., 55-55.
https://hdl.handle.net/21.15107/rcub_ibiss_4681

Kalezić A, Udicki M, Srdić Galić B, Korać A, Janković A, Korać B. Molecular basis of obesity and cancer. in 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia. 2021;:55-55.
https://hdl.handle.net/21.15107/rcub_ibiss_4681 .

Kalezić, Anđelika, Udicki, Mirjana, Srdić Galić, Biljana, Korać, Aleksandra, Janković, Aleksandra, Korać, Bato, "Molecular basis of obesity and cancer" in 14th International Congress on Nutrition: A Place Where Science Meets Practice, Book of Abstracts; 2021 Nov 8-10; Belgrade, Serbia (2021):55-55,
https://hdl.handle.net/21.15107/rcub_ibiss_4681 .

TY  - JOUR
AU  - Kalezić, Anđelika
AU  - Udicki, Mirjana
AU  - Srdić Galić, Biljana
AU  - Aleksić, Marija
AU  - Korać, Aleksandra
AU  - Janković, Aleksandra
AU  - Korać, Bato
PY  - 2020
UR  - https://www.mdpi.com/1422-0067/21/24/9676
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4082
AB  - Metabolic reprogramming that favors high glycolytic flux with lactate production in normoxia is among cancer hallmarks. Lactate is an essential oncometabolite regulating cellular redox homeostasis, energy substrate partitioning, and intracellular signaling. Moreover, malignant phenotype’s chief characteristics are dependent on the interaction between cancer cells and their microenvironment. In breast cancer, mammary adipocytes represent an essential cellular component of the tumor milieu. We analyzed lactate concentration, lactate dehydrogenase (LDH) activity, and isozyme pattern, and LDHA/LDHB protein expression and tissue localization in paired biopsies of breast cancer tissue and cancer-associated adipose tissue in normal-weight and overweight/obese premenopausal women, compared to benign breast tumor tissue and adipose tissue in normal-weight and overweight/obese premenopausal women. We show that higher lactate concentration in cancer tissue is concomitant with a shift in isozyme pattern towards the “muscle-type” LDH and corresponding LDHA and LDHB protein expression changes. In contrast, significantly higher LDH activity in cancer-associated adipose tissue seems to be directed towards lactate oxidation. Moreover, localization patterns of LDH isoforms varied substantially across different areas of breast cancer tissue. Invasive front of the tumor showed cell-specific protein localization of LDHA in breast cancer cells and LDHB in cancer-associated adipocytes. The results suggest a specific, lactate-centric relationship between cancer tissue and cancer-associated adipose tissue and indicate how cancer-adipose tissue cross-talk may be influenced by obesity in premenopausal women.
PB  - MDPI AG
T2  - International Journal of Molecular Sciences
T1  - Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity
IS  - 24
VL  - 21
DO  - 10.3390/ijms21249676
SP  - 9676
ER  - 

@article{
author = "Kalezić, Anđelika and Udicki, Mirjana and Srdić Galić, Biljana and Aleksić, Marija and Korać, Aleksandra and Janković, Aleksandra and Korać, Bato",
year = "2020",
abstract = "Metabolic reprogramming that favors high glycolytic flux with lactate production in normoxia is among cancer hallmarks. Lactate is an essential oncometabolite regulating cellular redox homeostasis, energy substrate partitioning, and intracellular signaling. Moreover, malignant phenotype’s chief characteristics are dependent on the interaction between cancer cells and their microenvironment. In breast cancer, mammary adipocytes represent an essential cellular component of the tumor milieu. We analyzed lactate concentration, lactate dehydrogenase (LDH) activity, and isozyme pattern, and LDHA/LDHB protein expression and tissue localization in paired biopsies of breast cancer tissue and cancer-associated adipose tissue in normal-weight and overweight/obese premenopausal women, compared to benign breast tumor tissue and adipose tissue in normal-weight and overweight/obese premenopausal women. We show that higher lactate concentration in cancer tissue is concomitant with a shift in isozyme pattern towards the “muscle-type” LDH and corresponding LDHA and LDHB protein expression changes. In contrast, significantly higher LDH activity in cancer-associated adipose tissue seems to be directed towards lactate oxidation. Moreover, localization patterns of LDH isoforms varied substantially across different areas of breast cancer tissue. Invasive front of the tumor showed cell-specific protein localization of LDHA in breast cancer cells and LDHB in cancer-associated adipocytes. The results suggest a specific, lactate-centric relationship between cancer tissue and cancer-associated adipose tissue and indicate how cancer-adipose tissue cross-talk may be influenced by obesity in premenopausal women.",
publisher = "MDPI AG",
journal = "International Journal of Molecular Sciences",
title = "Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity",
number = "24",
volume = "21",
doi = "10.3390/ijms21249676",
pages = "9676"
}

Kalezić, A., Udicki, M., Srdić Galić, B., Aleksić, M., Korać, A., Janković, A.,& Korać, B.. (2020). Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity. in International Journal of Molecular Sciences
MDPI AG., 21(24), 9676.
https://doi.org/10.3390/ijms21249676

Kalezić A, Udicki M, Srdić Galić B, Aleksić M, Korać A, Janković A, Korać B. Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity. in International Journal of Molecular Sciences. 2020;21(24):9676.
doi:10.3390/ijms21249676 .

Kalezić, Anđelika, Udicki, Mirjana, Srdić Galić, Biljana, Aleksić, Marija, Korać, Aleksandra, Janković, Aleksandra, Korać, Bato, "Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity" in International Journal of Molecular Sciences, 21, no. 24 (2020):9676,
https://doi.org/10.3390/ijms21249676 . .