TY  - CONF
AU  - Živković, Milica
AU  - Soković Bajić, Svetlana
AU  - Tolinački, Maja
AU  - Brdarić, Emilija
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Golić, Nataša
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5690
AB  - Objective
Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment
of diabetic rats showed decreased hyperglycemia and
ameliorating effect on diabetes-associated damage of liver and kidneys.
Hence, the aim of this study was to reveal the effects of BGCG11 probiotic
on gut microbiota composition and monitoring the insulin production in
pancreatic islets in diabetic rats.
Methods
Experiments were performed on albino Wistar rats divided into four groups:
ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes;
P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were
orally administered with BGCG11, one week before (P/D/P) and after the
STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from
all fecal samples and rDNA amplicons were analyzed by DGGE and 16S
rDNA genes sequencing. For immunohistochemical analysis, slides were
stained with anti-insulin antibody and secondary antibody coupled with
horseradish peroxidase.
Results
The results revealed the higher diversity of gut microbiota in D/P group
comparing to D group, as well as the higher prevalence of Flintibacter
butyricus (the major butyric producer), Acetatifactor muris (present in
obese mouse) and Eisenbergiella massiliensis (found in obese woman),
while the lipolytic bacterium Aestuariispira insulae was more prevalent in
diabetic rats. In both, P/D/P and D/P group, increased number of positive
immunoreactions of β-cells for anti-insulin antibodies was displayed in
compare to D group with islet atrophy.
Conclusions
The results of this study suggest that the positive effect of BGCG11 on
STZ-induced diabetes in rats could be annotated to its protective role on
the integrity of fecal microbiota.
PB  - Wolters Kluwer Health
C3  - 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
T1  - The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11
DO  - 10.1097/MCG.0000000000001292
SP  - 103
ER  - 

@conference{
author = "Živković, Milica and Soković Bajić, Svetlana and Tolinački, Maja and Brdarić, Emilija and Đokić, Jelena and Popović, Nikola and Rajić, Jovana and Đorđević, Marija and Golić, Nataša",
year = "2019",
abstract = "Objective
Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment
of diabetic rats showed decreased hyperglycemia and
ameliorating effect on diabetes-associated damage of liver and kidneys.
Hence, the aim of this study was to reveal the effects of BGCG11 probiotic
on gut microbiota composition and monitoring the insulin production in
pancreatic islets in diabetic rats.
Methods
Experiments were performed on albino Wistar rats divided into four groups:
ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes;
P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were
orally administered with BGCG11, one week before (P/D/P) and after the
STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from
all fecal samples and rDNA amplicons were analyzed by DGGE and 16S
rDNA genes sequencing. For immunohistochemical analysis, slides were
stained with anti-insulin antibody and secondary antibody coupled with
horseradish peroxidase.
Results
The results revealed the higher diversity of gut microbiota in D/P group
comparing to D group, as well as the higher prevalence of Flintibacter
butyricus (the major butyric producer), Acetatifactor muris (present in
obese mouse) and Eisenbergiella massiliensis (found in obese woman),
while the lipolytic bacterium Aestuariispira insulae was more prevalent in
diabetic rats. In both, P/D/P and D/P group, increased number of positive
immunoreactions of β-cells for anti-insulin antibodies was displayed in
compare to D group with islet atrophy.
Conclusions
The results of this study suggest that the positive effect of BGCG11 on
STZ-induced diabetes in rats could be annotated to its protective role on
the integrity of fecal microbiota.",
publisher = "Wolters Kluwer Health",
journal = "10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy",
title = "The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11",
doi = "10.1097/MCG.0000000000001292",
pages = "103"
}

Živković, M., Soković Bajić, S., Tolinački, M., Brdarić, E., Đokić, J., Popović, N., Rajić, J., Đorđević, M.,& Golić, N.. (2019). The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
Wolters Kluwer Health., 103.
https://doi.org/10.1097/MCG.0000000000001292

Živković M, Soković Bajić S, Tolinački M, Brdarić E, Đokić J, Popović N, Rajić J, Đorđević M, Golić N. The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy. 2019;:103.
doi:10.1097/MCG.0000000000001292 .

Živković, Milica, Soković Bajić, Svetlana, Tolinački, Maja, Brdarić, Emilija, Đokić, Jelena, Popović, Nikola, Rajić, Jovana, Đorđević, Marija, Golić, Nataša, "The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11" in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy (2019):103,
https://doi.org/10.1097/MCG.0000000000001292 . .

TY  - JOUR
AU  - Stanisavljević, Suzana
AU  - Dinić, Miroslav
AU  - Jevtić, Bojan
AU  - Nikolovski, Neda
AU  - Momčilović, Miljana
AU  - Đokić, Jelena
AU  - Golić, Nataša
AU  - Mostarica Stojković, Marija
AU  - Miljković, Đorđe
PY  - 2018
UR  - http://journal.frontiersin.org/article/10.3389/fimmu.2018.00942/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5942155
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3056
AB  - Albino Oxford (AO) rats are extremely resistant to induction of experimental autoimmune encephalomyelitis (EAE). EAE is an animal model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS), with established autoimmune pathogenesis. The autoimmune response against the antigens of the CNS is initiated in the peripheral lymphoid tissues after immunization of AO rats with CNS antigens. Subsequently, limited infiltration of the CNS occurs, yet without clinical sequels. It has recently become increasingly appreciated that gut-associated lymphoid tissues (GALT) and gut microbiota play an important role in regulation and propagation of encephalitogenic immune response. Therefore, modulation of AO gut microbiota by antibiotics was performed in this study. The treatment altered composition of gut microbiota in AO rats and led to a reduction in the proportion of regulatory T cells in Peyer's patches, mesenteric lymph nodes, and in lymph nodes draining the site of immunization. Upregulation of interferon-γ and interleukin (IL)-17 production was observed in the draining lymph nodes. The treatment led to clinically manifested EAE in AO rats with more numerous infiltrates and higher production of IL-17 observed in the CNS. Importantly, transfer of AO gut microbiota into EAE-prone Dark Agouti rats ameliorated the disease. These results clearly imply that gut microbiota is an important factor in AO rat resistance to EAE and that gut microbiota transfer is an efficacious way to treat CNS autoimmunity. These findings also support the idea that gut microbiota modulation has a potential as a future treatment of multiple sclerosis.
T2  - Frontiers in Immunology
T1  - Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis.
VL  - 9
DO  - 10.3389/fimmu.2018.00942
SP  - 942
ER  - 

@article{
author = "Stanisavljević, Suzana and Dinić, Miroslav and Jevtić, Bojan and Nikolovski, Neda and Momčilović, Miljana and Đokić, Jelena and Golić, Nataša and Mostarica Stojković, Marija and Miljković, Đorđe",
year = "2018",
abstract = "Albino Oxford (AO) rats are extremely resistant to induction of experimental autoimmune encephalomyelitis (EAE). EAE is an animal model of multiple sclerosis, a chronic inflammatory disease of the central nervous system (CNS), with established autoimmune pathogenesis. The autoimmune response against the antigens of the CNS is initiated in the peripheral lymphoid tissues after immunization of AO rats with CNS antigens. Subsequently, limited infiltration of the CNS occurs, yet without clinical sequels. It has recently become increasingly appreciated that gut-associated lymphoid tissues (GALT) and gut microbiota play an important role in regulation and propagation of encephalitogenic immune response. Therefore, modulation of AO gut microbiota by antibiotics was performed in this study. The treatment altered composition of gut microbiota in AO rats and led to a reduction in the proportion of regulatory T cells in Peyer's patches, mesenteric lymph nodes, and in lymph nodes draining the site of immunization. Upregulation of interferon-γ and interleukin (IL)-17 production was observed in the draining lymph nodes. The treatment led to clinically manifested EAE in AO rats with more numerous infiltrates and higher production of IL-17 observed in the CNS. Importantly, transfer of AO gut microbiota into EAE-prone Dark Agouti rats ameliorated the disease. These results clearly imply that gut microbiota is an important factor in AO rat resistance to EAE and that gut microbiota transfer is an efficacious way to treat CNS autoimmunity. These findings also support the idea that gut microbiota modulation has a potential as a future treatment of multiple sclerosis.",
journal = "Frontiers in Immunology",
title = "Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis.",
volume = "9",
doi = "10.3389/fimmu.2018.00942",
pages = "942"
}

Stanisavljević, S., Dinić, M., Jevtić, B., Nikolovski, N., Momčilović, M., Đokić, J., Golić, N., Mostarica Stojković, M.,& Miljković, Đ.. (2018). Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis.. in Frontiers in Immunology, 9, 942.
https://doi.org/10.3389/fimmu.2018.00942

Stanisavljević S, Dinić M, Jevtić B, Nikolovski N, Momčilović M, Đokić J, Golić N, Mostarica Stojković M, Miljković Đ. Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis.. in Frontiers in Immunology. 2018;9:942.
doi:10.3389/fimmu.2018.00942 .

Stanisavljević, Suzana, Dinić, Miroslav, Jevtić, Bojan, Nikolovski, Neda, Momčilović, Miljana, Đokić, Jelena, Golić, Nataša, Mostarica Stojković, Marija, Miljković, Đorđe, "Gut Microbiota Confers Resistance of Albino Oxford Rats to the Induction of Experimental Autoimmune Encephalomyelitis." in Frontiers in Immunology, 9 (2018):942,
https://doi.org/10.3389/fimmu.2018.00942 . .

TY  - JOUR
AU  - Đokić, Jelena
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Subota, Vesna
AU  - Mihajlović, Luka
AU  - Stojadinović, Marija
AU  - Stanić-Vučinić, Dragana
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/566
AB  - Warfarin (3-(α-acetonylbenzyl)-4-hydroxy coumarin) is a vitamin K (VK) antagonist that inhibits vitamin K-dependent (VKD) processes, such as blood coagulation. It also exerts an influence on some non-VKD-related activities. In this study, the effect of sub-acute (30-day) oral warfarin (2 and 1 mg L-1) intake on hematological parameters was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), that differ in their sensitivity to certain chemicals. Greater susceptibility to the anticoagulant effect of 2 mg L-1 of warfarin was observed in AO rats and was associated with an increase in the relevant hematological parameters in this strain. Although both strains responded to 2 mg L-1 of warfarin with quantitative changes in the peripheral blood leukocytes, differential bone marrow and lung responses were observed. Strain-related differences in the pro-inflammatory activity of peripheral blood granulocytes and in mononuclear cell IFN-γ production were observed. Recognition of differences in quantitative and qualitative effects of oral warfarin on processes other than hemostasis might be of relevance for those humans who are on warfarin therapy.
AB  - Varfarin (3-α-acetonilbenzil)-4–hidroksikumarin) je antagonist vitamina K (VK) koji inhibira procese zavisne od ovog vitamina, uključujući koagulaciju krvi. Osim toga, on ispoljava i aktivnosti koje ne zavise od vitamina K kao što su anti-tumorska i imunomodulatorna aktivnost. U ovom radu je ispitan efekat subakutnog (30 dana) oralnog unosa varfarina na hematološke parametre i aktivnost leukocita periferne krvi kod dva soja pacova Albino Oxford (AO) i Dark Agouti (DA) koji se raz- likuju u osetljivosti na iste hemijske agense. Kod jedinki AO soja zapažena je veća smrtnost nakon konzumiranja doze od 4 mg L–1 kao i veća osetljivost na antikoagulantno dejstvo varfarina pri nižim dozama (2 mg L–1) koje je praćeno povećanjem nekih hematoloških parametara. Iako kod jedinki oba soja dolazi do povećanja broja neutrofilnih leukocita periferne krvi pri dozi od 2 mg L–1, promene u osnovnim proinflamatornim aktivnostima ovih ćelija su zapažene samo kod jedinki DA soja. Promene u broju neutrofilnih leukocita u krvi DA jedinki su praćene povećanjem broja granulocitnih prekursora u koštanoj srži, dok prisustvo neutrofila u plućima AO jedinki ukazuje na razmenu ćelija između periferne krvi i plućnog intravaskularnog pula ćelija. Diferencijalne sojno–zavisne promene u aktivnosti mononuklearnih ćelija periferne krvi su takođe zapažene. Razlike u efektu oralno unetog varfarina mogu da imaju implikacije za osobe na oralnoj varfarinskoj terapiji.
T2  - Journal of the Serbian Chemical Society
T1  - Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova
T1  - Strain differences in the toxicity of the vitamin K antagonist warfarin in rats
IS  - 3
VL  - 78
SP  - 381
EP  - 394
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_566
ER  - 

@article{
author = "Đokić, Jelena and Ninkov, Marina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Subota, Vesna and Mihajlović, Luka and Stojadinović, Marija and Stanić-Vučinić, Dragana and Kataranovski, Dragan and Kataranovski, Milena",
year = "2013, 2013",
abstract = "Warfarin (3-(α-acetonylbenzyl)-4-hydroxy coumarin) is a vitamin K (VK) antagonist that inhibits vitamin K-dependent (VKD) processes, such as blood coagulation. It also exerts an influence on some non-VKD-related activities. In this study, the effect of sub-acute (30-day) oral warfarin (2 and 1 mg L-1) intake on hematological parameters was examined in two rat strains, Albino Oxford (AO) and Dark Agouti (DA), that differ in their sensitivity to certain chemicals. Greater susceptibility to the anticoagulant effect of 2 mg L-1 of warfarin was observed in AO rats and was associated with an increase in the relevant hematological parameters in this strain. Although both strains responded to 2 mg L-1 of warfarin with quantitative changes in the peripheral blood leukocytes, differential bone marrow and lung responses were observed. Strain-related differences in the pro-inflammatory activity of peripheral blood granulocytes and in mononuclear cell IFN-γ production were observed. Recognition of differences in quantitative and qualitative effects of oral warfarin on processes other than hemostasis might be of relevance for those humans who are on warfarin therapy., Varfarin (3-α-acetonilbenzil)-4–hidroksikumarin) je antagonist vitamina K (VK) koji inhibira procese zavisne od ovog vitamina, uključujući koagulaciju krvi. Osim toga, on ispoljava i aktivnosti koje ne zavise od vitamina K kao što su anti-tumorska i imunomodulatorna aktivnost. U ovom radu je ispitan efekat subakutnog (30 dana) oralnog unosa varfarina na hematološke parametre i aktivnost leukocita periferne krvi kod dva soja pacova Albino Oxford (AO) i Dark Agouti (DA) koji se raz- likuju u osetljivosti na iste hemijske agense. Kod jedinki AO soja zapažena je veća smrtnost nakon konzumiranja doze od 4 mg L–1 kao i veća osetljivost na antikoagulantno dejstvo varfarina pri nižim dozama (2 mg L–1) koje je praćeno povećanjem nekih hematoloških parametara. Iako kod jedinki oba soja dolazi do povećanja broja neutrofilnih leukocita periferne krvi pri dozi od 2 mg L–1, promene u osnovnim proinflamatornim aktivnostima ovih ćelija su zapažene samo kod jedinki DA soja. Promene u broju neutrofilnih leukocita u krvi DA jedinki su praćene povećanjem broja granulocitnih prekursora u koštanoj srži, dok prisustvo neutrofila u plućima AO jedinki ukazuje na razmenu ćelija između periferne krvi i plućnog intravaskularnog pula ćelija. Diferencijalne sojno–zavisne promene u aktivnosti mononuklearnih ćelija periferne krvi su takođe zapažene. Razlike u efektu oralno unetog varfarina mogu da imaju implikacije za osobe na oralnoj varfarinskoj terapiji.",
journal = "Journal of the Serbian Chemical Society",
title = "Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova, Strain differences in the toxicity of the vitamin K antagonist warfarin in rats",
number = "3",
volume = "78",
pages = "381-394",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_566"
}

Đokić, J., Ninkov, M., Popov Aleksandrov, A., Mirkov, I., Subota, V., Mihajlović, L., Stojadinović, M., Stanić-Vučinić, D., Kataranovski, D.,& Kataranovski, M.. (2013). Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova. in Journal of the Serbian Chemical Society, 78(3), 381-394.
https://hdl.handle.net/21.15107/rcub_ibiss_566

Đokić J, Ninkov M, Popov Aleksandrov A, Mirkov I, Subota V, Mihajlović L, Stojadinović M, Stanić-Vučinić D, Kataranovski D, Kataranovski M. Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova. in Journal of the Serbian Chemical Society. 2013;78(3):381-394.
https://hdl.handle.net/21.15107/rcub_ibiss_566 .

Đokić, Jelena, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Subota, Vesna, Mihajlović, Luka, Stojadinović, Marija, Stanić-Vučinić, Dragana, Kataranovski, Dragan, Kataranovski, Milena, "Sojne razlike u toksičnosti antagoniste vitamina K varfarina kod pacova" in Journal of the Serbian Chemical Society, 78, no. 3 (2013):381-394,
https://hdl.handle.net/21.15107/rcub_ibiss_566 .

TY  - JOUR
AU  - El-Muzghi, Amal Atia Mhfuod
AU  - Mirkov, Ivana
AU  - Đokić, Jelena
AU  - Popov Aleksandrov, Aleksandra
AU  - Miljković, Đorđe
AU  - Glamočlija, Jasmina
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/945
AB  - Rat models of pulmonary aspergillosis are used widely in diagnostic studies and in exploring antifungal therapeutic modalities, but there is lack of data concerning antifungal immunity in rats: In this study, cytokine response to pulmonary infection to Aspergillus fumigatus in non-immunosuppressed rats is explored. Temporal display (from the start of infection up to its eradication) of proinflammatory cytokines (IFN-gamma and IL-17) as well as Th2/anti-inflammatory ones (IL-4 and IL-10) was explored by measuring their presence in the environment in which elimination of infection occur (lung homogenates), by production of these mediators by lung cells (recovered by enzyme digestion or by bronchoalveolar lavage) as well as by cells of draining lymph nodes (as sites of generation of cytokine-producing cells). Reduction of infection (1 x 10(7) conidia) was associated with an increase of IFN-gamma and IL-17 content in lung homogenates, but with unchanged IL-4 and IL-10 content. Lung cells produced proinflammatory cytokines with differential dynamics (IFN-gamma earlier than IL-17). Differential pattern of Th2/anti-inflammatory cytokine production by lung cells was observed (unchanged IL-4 and increased IL-10), with the levels of the latter higher than proinflammatory cytokines. Upregulation of IFN-gamma, IL-17 and IL-10 production and gene expression, but downregulation of IL-4, by draining lymph node cells (dLN cells) accounted essentially for the observed ex vivo cytokine response in lungs. Similar pattern of cytokine production by dLN cells following restimulation with A. fumigatus conidia confirmed the specificity of cytokine response to the fungus. Draining lymph node CD4(+) cells seems to be the main source of proinflammatory cytokines, significant contributors to IL-10 production and the target for down regulation of IL-4. The knowledge of immune-based mechanisms of defense against A. fumigatus in rats might be helpful in the future use of rat models of pulmonary aspergillosis particularly those that develop immune-based therapeutic interventions as an adjunct treatment of fungal diseases. (C) 2013 Elsevier GmbH. All rights reserved.
T2  - Immunobiology
T1  - Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats
IS  - 12
VL  - 218
SP  - 69
EP  - 1523
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_945
ER  - 

@article{
author = "El-Muzghi, Amal Atia Mhfuod and Mirkov, Ivana and Đokić, Jelena and Popov Aleksandrov, Aleksandra and Miljković, Đorđe and Glamočlija, Jasmina and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2013",
abstract = "Rat models of pulmonary aspergillosis are used widely in diagnostic studies and in exploring antifungal therapeutic modalities, but there is lack of data concerning antifungal immunity in rats: In this study, cytokine response to pulmonary infection to Aspergillus fumigatus in non-immunosuppressed rats is explored. Temporal display (from the start of infection up to its eradication) of proinflammatory cytokines (IFN-gamma and IL-17) as well as Th2/anti-inflammatory ones (IL-4 and IL-10) was explored by measuring their presence in the environment in which elimination of infection occur (lung homogenates), by production of these mediators by lung cells (recovered by enzyme digestion or by bronchoalveolar lavage) as well as by cells of draining lymph nodes (as sites of generation of cytokine-producing cells). Reduction of infection (1 x 10(7) conidia) was associated with an increase of IFN-gamma and IL-17 content in lung homogenates, but with unchanged IL-4 and IL-10 content. Lung cells produced proinflammatory cytokines with differential dynamics (IFN-gamma earlier than IL-17). Differential pattern of Th2/anti-inflammatory cytokine production by lung cells was observed (unchanged IL-4 and increased IL-10), with the levels of the latter higher than proinflammatory cytokines. Upregulation of IFN-gamma, IL-17 and IL-10 production and gene expression, but downregulation of IL-4, by draining lymph node cells (dLN cells) accounted essentially for the observed ex vivo cytokine response in lungs. Similar pattern of cytokine production by dLN cells following restimulation with A. fumigatus conidia confirmed the specificity of cytokine response to the fungus. Draining lymph node CD4(+) cells seems to be the main source of proinflammatory cytokines, significant contributors to IL-10 production and the target for down regulation of IL-4. The knowledge of immune-based mechanisms of defense against A. fumigatus in rats might be helpful in the future use of rat models of pulmonary aspergillosis particularly those that develop immune-based therapeutic interventions as an adjunct treatment of fungal diseases. (C) 2013 Elsevier GmbH. All rights reserved.",
journal = "Immunobiology",
title = "Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats",
number = "12",
volume = "218",
pages = "69-1523",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_945"
}

El-Muzghi, A. A. M., Mirkov, I., Đokić, J., Popov Aleksandrov, A., Miljković, Đ., Glamočlija, J., Kataranovski, D. S.,& Kataranovski, M.. (2013). Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats. in Immunobiology, 218(12), 69-1523.
https://hdl.handle.net/21.15107/rcub_ibiss_945

El-Muzghi AAM, Mirkov I, Đokić J, Popov Aleksandrov A, Miljković Đ, Glamočlija J, Kataranovski DS, Kataranovski M. Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats. in Immunobiology. 2013;218(12):69-1523.
https://hdl.handle.net/21.15107/rcub_ibiss_945 .

El-Muzghi, Amal Atia Mhfuod, Mirkov, Ivana, Đokić, Jelena, Popov Aleksandrov, Aleksandra, Miljković, Đorđe, Glamočlija, Jasmina, Kataranovski, Dragan S., Kataranovski, Milena, "Regional cytokine responses to pulmonary aspergillosis in immunocompetent rats" in Immunobiology, 218, no. 12 (2013):69-1523,
https://hdl.handle.net/21.15107/rcub_ibiss_945 .

TY  - CONF
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Miljković, Đorđe
AU  - Belij, Sandra
AU  - Đokić, Jelena
AU  - Zolotarevski, Lidija D
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1113
C3  - Immunology
T1  - Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats
IS  - null
VL  - 137
SP  - 349
EP  - 442
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1113
ER  - 

@conference{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Miljković, Đorđe and Belij, Sandra and Đokić, Jelena and Zolotarevski, Lidija D and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
journal = "Immunology",
title = "Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats",
number = "null",
volume = "137",
pages = "349-442",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1113"
}

Popov Aleksandrov, A., Mirkov, I., Miljković, Đ., Belij, S., Đokić, J., Zolotarevski, L. D., Kataranovski, D. S.,& Kataranovski, M.. (2012). Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats. in Immunology, 137(null), 349-442.
https://hdl.handle.net/21.15107/rcub_ibiss_1113

Popov Aleksandrov A, Mirkov I, Miljković Đ, Belij S, Đokić J, Zolotarevski LD, Kataranovski DS, Kataranovski M. Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats. in Immunology. 2012;137(null):349-442.
https://hdl.handle.net/21.15107/rcub_ibiss_1113 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Miljković, Đorđe, Belij, Sandra, Đokić, Jelena, Zolotarevski, Lidija D, Kataranovski, Dragan S., Kataranovski, Milena, "Contribution of sensitization phase to intensity of contact hypersensitivity reaction in rats" in Immunology, 137, no. null (2012):349-442,
https://hdl.handle.net/21.15107/rcub_ibiss_1113 .

TY  - JOUR
AU  - Belij, Sandra
AU  - Popov Aleksandrov, Aleksandra
AU  - Zolotarevski, Lidija D
AU  - Mirkov, Ivana
AU  - Đokić, Jelena
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1203
AB  - Topical application of dinitrochlorobenzene (DNCB) is employed in the immunotherapy of skin diseases. Activation of T-cell mediated immune responses (Th1/type1) is the supposed mechanism of the clinical effect of DNCB, but there are no data concerning innate/inflammatory mechanisms. In this study, the effect of repeated topical DNCB application on peripheral blood polymorphonuclear (PMN) leukocytes has been examined in two rat strains which differ in the propensity to mount Th1/type1 or Th2/type2 responses. The dynamics of changes in PMN numbers and effector activities (respiratory burst, nitric oxide production and myeloperoxidase content), as well as in adhesion and TNF-alpha production following the rat skin sensitization with low (0.4%) and high (4%) DNCB doses were measured. Both priming and activation of PMNs were observed following skin sensitization with DNCB, with dose-dependent as well as time-dependent differences in some PMN activities. Obtained data might be relevant for understanding the immune mechanisms of topical DNCB therapy. (C) 2011 Published by Elsevier B.V.
T2  - Environmental Toxicology and Pharmacology
T1  - Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells
IS  - 2
VL  - 33
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1203
ER  - 

@article{
author = "Belij, Sandra and Popov Aleksandrov, Aleksandra and Zolotarevski, Lidija D and Mirkov, Ivana and Đokić, Jelena and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
abstract = "Topical application of dinitrochlorobenzene (DNCB) is employed in the immunotherapy of skin diseases. Activation of T-cell mediated immune responses (Th1/type1) is the supposed mechanism of the clinical effect of DNCB, but there are no data concerning innate/inflammatory mechanisms. In this study, the effect of repeated topical DNCB application on peripheral blood polymorphonuclear (PMN) leukocytes has been examined in two rat strains which differ in the propensity to mount Th1/type1 or Th2/type2 responses. The dynamics of changes in PMN numbers and effector activities (respiratory burst, nitric oxide production and myeloperoxidase content), as well as in adhesion and TNF-alpha production following the rat skin sensitization with low (0.4%) and high (4%) DNCB doses were measured. Both priming and activation of PMNs were observed following skin sensitization with DNCB, with dose-dependent as well as time-dependent differences in some PMN activities. Obtained data might be relevant for understanding the immune mechanisms of topical DNCB therapy. (C) 2011 Published by Elsevier B.V.",
journal = "Environmental Toxicology and Pharmacology",
title = "Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells",
number = "2",
volume = "33",
pages = "180",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1203"
}

Belij, S., Popov Aleksandrov, A., Zolotarevski, L. D., Mirkov, I., Đokić, J., Kataranovski, D. S.,& Kataranovski, M.. (2012). Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells. in Environmental Toxicology and Pharmacology, 33(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1203

Belij S, Popov Aleksandrov A, Zolotarevski LD, Mirkov I, Đokić J, Kataranovski DS, Kataranovski M. Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells. in Environmental Toxicology and Pharmacology. 2012;33(2):null-180.
https://hdl.handle.net/21.15107/rcub_ibiss_1203 .

Belij, Sandra, Popov Aleksandrov, Aleksandra, Zolotarevski, Lidija D, Mirkov, Ivana, Đokić, Jelena, Kataranovski, Dragan S., Kataranovski, Milena, "Systemic immunomodulatory effects of topical dinitrochlorobenzene (DNCB) in rats. Activity of peripheral blood polymorphonuclear cells" in Environmental Toxicology and Pharmacology, 33, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1203 .

TY  - CONF
AU  - Mirkov, Ivana
AU  - El-Muzghi, Amal Atia Mhfuod
AU  - Popov Aleksandrov, Aleksandra
AU  - Đokić, Jelena
AU  - Miljković, Đorđe
AU  - Glamočlija, Jasmina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4101
PB  - Odbor za imunologiju i alergologiju odeljenja medicinskih nauka SANU
C3  - Svestski dan imunologije 2012
T1  - Imunski mehanizmi u rezistenciji na A. fumigatus
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4101
ER  - 

@conference{
author = "Mirkov, Ivana and El-Muzghi, Amal Atia Mhfuod and Popov Aleksandrov, Aleksandra and Đokić, Jelena and Miljković, Đorđe and Glamočlija, Jasmina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2012",
publisher = "Odbor za imunologiju i alergologiju odeljenja medicinskih nauka SANU",
journal = "Svestski dan imunologije 2012",
title = "Imunski mehanizmi u rezistenciji na A. fumigatus",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4101"
}

Mirkov, I., El-Muzghi, A. A. M., Popov Aleksandrov, A., Đokić, J., Miljković, Đ., Glamočlija, J., Kataranovski, D.,& Kataranovski, M.. (2012). Imunski mehanizmi u rezistenciji na A. fumigatus. in Svestski dan imunologije 2012
Odbor za imunologiju i alergologiju odeljenja medicinskih nauka SANU..
https://hdl.handle.net/21.15107/rcub_ibiss_4101

Mirkov I, El-Muzghi AAM, Popov Aleksandrov A, Đokić J, Miljković Đ, Glamočlija J, Kataranovski D, Kataranovski M. Imunski mehanizmi u rezistenciji na A. fumigatus. in Svestski dan imunologije 2012. 2012;.
https://hdl.handle.net/21.15107/rcub_ibiss_4101 .

Mirkov, Ivana, El-Muzghi, Amal Atia Mhfuod, Popov Aleksandrov, Aleksandra, Đokić, Jelena, Miljković, Đorđe, Glamočlija, Jasmina, Kataranovski, Dragan, Kataranovski, Milena, "Imunski mehanizmi u rezistenciji na A. fumigatus" in Svestski dan imunologije 2012 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_4101 .