TY  - JOUR
AU  - Selaković, Vesna
AU  - Petković, Branka
AU  - Radenović, Lidija
PY  - 2010
UR  - https://link.springer.com/article/10.1007%2Fs11010-010-0466-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3797
AB  - We investigated MK-801 effect on ischemia-induced oxidative stress-the most important factor that exacerbates brain damage by reperfusion. The common carotid arteries of gerbils were occluded for 5, 10, or 15 min. Immediately after the occlusion, MK-801 (3 mg/kg i.p.) or saline were given in normothermic conditions. The MK-801 effects were followed in vivo by monitoring the neurological status of animals and at the intracellular level by standard biochemical assays. We investigated nitric oxide levels, superoxide production, superoxide dismutase activity, index of lipid peroxidation (ILP), and reduced glutathione content in hippocampus, striatum, forebrain cortex, and cerebellum. The measurements took place at different times (1, 2, 4, 7, 14, and 28 days) after reperfusion. Increased duration of cerebral ischemia resulted in a progressive induction of oxidative stress. Our results revealed pattern of dynamic changes in each oxidative stress parameter level which corresponded with ischemia duration in all tested brain structures. Most sensitive oxidative stress parameters were ILP and superoxide production. Our study confirmed spatial distribution of ischemia-induced oxidative stress. Tested brain structures showed different sensitivity to each oxidative stress parameter. As judged by biochemical and neurological data, applied MK-801 showed neuroprotective efficiency by reduction of ischemia-induced oxidative stress in brain.
PB  - Springer
T2  - Molecular and Cellular Biochemistry
T1  - MK-801 effect on regional cerebral oxidative stress rate induced by different duration of global ischemia in gerbils
IS  - 1-2
VL  - 342
DO  - 10.1007/s11010-010-0466-x
SP  - 35
EP  - 50
ER  - 

@article{
author = "Selaković, Vesna and Petković, Branka and Radenović, Lidija",
year = "2010",
abstract = "We investigated MK-801 effect on ischemia-induced oxidative stress-the most important factor that exacerbates brain damage by reperfusion. The common carotid arteries of gerbils were occluded for 5, 10, or 15 min. Immediately after the occlusion, MK-801 (3 mg/kg i.p.) or saline were given in normothermic conditions. The MK-801 effects were followed in vivo by monitoring the neurological status of animals and at the intracellular level by standard biochemical assays. We investigated nitric oxide levels, superoxide production, superoxide dismutase activity, index of lipid peroxidation (ILP), and reduced glutathione content in hippocampus, striatum, forebrain cortex, and cerebellum. The measurements took place at different times (1, 2, 4, 7, 14, and 28 days) after reperfusion. Increased duration of cerebral ischemia resulted in a progressive induction of oxidative stress. Our results revealed pattern of dynamic changes in each oxidative stress parameter level which corresponded with ischemia duration in all tested brain structures. Most sensitive oxidative stress parameters were ILP and superoxide production. Our study confirmed spatial distribution of ischemia-induced oxidative stress. Tested brain structures showed different sensitivity to each oxidative stress parameter. As judged by biochemical and neurological data, applied MK-801 showed neuroprotective efficiency by reduction of ischemia-induced oxidative stress in brain.",
publisher = "Springer",
journal = "Molecular and Cellular Biochemistry",
title = "MK-801 effect on regional cerebral oxidative stress rate induced by different duration of global ischemia in gerbils",
number = "1-2",
volume = "342",
doi = "10.1007/s11010-010-0466-x",
pages = "35-50"
}

Selaković, V., Petković, B.,& Radenović, L.. (2010). MK-801 effect on regional cerebral oxidative stress rate induced by different duration of global ischemia in gerbils. in Molecular and Cellular Biochemistry
Springer., 342(1-2), 35-50.
https://doi.org/10.1007/s11010-010-0466-x

Selaković V, Petković B, Radenović L. MK-801 effect on regional cerebral oxidative stress rate induced by different duration of global ischemia in gerbils. in Molecular and Cellular Biochemistry. 2010;342(1-2):35-50.
doi:10.1007/s11010-010-0466-x .

Selaković, Vesna, Petković, Branka, Radenović, Lidija, "MK-801 effect on regional cerebral oxidative stress rate induced by different duration of global ischemia in gerbils" in Molecular and Cellular Biochemistry, 342, no. 1-2 (2010):35-50,
https://doi.org/10.1007/s11010-010-0466-x . .

TY  - JOUR
AU  - Petković, Branka
AU  - Tovilović-Kovačević, Gordana
AU  - Tomić, Mirko
AU  - Prolić, Zlatko M.
AU  - Radenović, Lidija Lj
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1472
UR  - http://www.gpb.sav.sk/2009-Special-Issue.htm
AB  - External magnetic fields (MFs) have the ability to modify motor activity of animals, complex type of behaviour connected with dopaminergic and serotonergic neurotransmissions in the brain. Thus, the purpose of this study was to examine MF-induced changes in the activity of serotonin 5-HT(2A) receptors in the prefrontal cortex, as well as dopamine D(1) and D(2) receptors in the striatum of adult Wistar rats, considering their involvement in motor behavior regulation. Experimental animals were continuously exposed to extremely low frequency MF (ELF-MF, 50 Hz, 0.5 mT) for 1, 3, and 7 days. Subsequently, binding properties (K(d) and B(max)) of receptors were determined by in vitro radioligand receptor binding assays. It was shown that the affinity of serotonin 5-HT(2A) receptors decreased and their density increased in the prefrontal cortex of rats after ELF-MF exposure. Regarding affinity, this effect was duration-dependent and most prominent after 7-day of ELF-MF exposure. In contrast to serotonin 5-HT(2A) receptors in the prefrontal cortex, ELF-MF had no significant effect on the affinity and density of dopamine D(1) and D(2) receptors in the striatum. We can conclude that continuous exposure to ELF-MF up to 7 days affects cortical serotonergic neurotransmission, whereby intensity of these changes depends on ELF-MF exposure duration.
PB  - Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences
T2  - General Physiology and Biophysics
T1  - Effect of continuous exposure to alternating magnetic field (50 Hz, 0.5 mT) on serotonin and dopamine receptors activity in rat brain
IS  - Special Issue
VL  - 28
SP  - 41
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1472
ER  - 

@article{
author = "Petković, Branka and Tovilović-Kovačević, Gordana and Tomić, Mirko and Prolić, Zlatko M. and Radenović, Lidija Lj",
year = "2009",
abstract = "External magnetic fields (MFs) have the ability to modify motor activity of animals, complex type of behaviour connected with dopaminergic and serotonergic neurotransmissions in the brain. Thus, the purpose of this study was to examine MF-induced changes in the activity of serotonin 5-HT(2A) receptors in the prefrontal cortex, as well as dopamine D(1) and D(2) receptors in the striatum of adult Wistar rats, considering their involvement in motor behavior regulation. Experimental animals were continuously exposed to extremely low frequency MF (ELF-MF, 50 Hz, 0.5 mT) for 1, 3, and 7 days. Subsequently, binding properties (K(d) and B(max)) of receptors were determined by in vitro radioligand receptor binding assays. It was shown that the affinity of serotonin 5-HT(2A) receptors decreased and their density increased in the prefrontal cortex of rats after ELF-MF exposure. Regarding affinity, this effect was duration-dependent and most prominent after 7-day of ELF-MF exposure. In contrast to serotonin 5-HT(2A) receptors in the prefrontal cortex, ELF-MF had no significant effect on the affinity and density of dopamine D(1) and D(2) receptors in the striatum. We can conclude that continuous exposure to ELF-MF up to 7 days affects cortical serotonergic neurotransmission, whereby intensity of these changes depends on ELF-MF exposure duration.",
publisher = "Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences",
journal = "General Physiology and Biophysics",
title = "Effect of continuous exposure to alternating magnetic field (50 Hz, 0.5 mT) on serotonin and dopamine receptors activity in rat brain",
number = "Special Issue",
volume = "28",
pages = "41-46",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1472"
}

Petković, B., Tovilović-Kovačević, G., Tomić, M., Prolić, Z. M.,& Radenović, L. L.. (2009). Effect of continuous exposure to alternating magnetic field (50 Hz, 0.5 mT) on serotonin and dopamine receptors activity in rat brain. in General Physiology and Biophysics
Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences., 28(Special Issue), 41-46.
https://hdl.handle.net/21.15107/rcub_ibiss_1472

Petković B, Tovilović-Kovačević G, Tomić M, Prolić ZM, Radenović LL. Effect of continuous exposure to alternating magnetic field (50 Hz, 0.5 mT) on serotonin and dopamine receptors activity in rat brain. in General Physiology and Biophysics. 2009;28(Special Issue):41-46.
https://hdl.handle.net/21.15107/rcub_ibiss_1472 .

Petković, Branka, Tovilović-Kovačević, Gordana, Tomić, Mirko, Prolić, Zlatko M., Radenović, Lidija Lj, "Effect of continuous exposure to alternating magnetic field (50 Hz, 0.5 mT) on serotonin and dopamine receptors activity in rat brain" in General Physiology and Biophysics, 28, no. Special Issue (2009):41-46,
https://hdl.handle.net/21.15107/rcub_ibiss_1472 .