TY  - JOUR
AU  - Brdarić, Emilija
AU  - Popović, Dušanka
AU  - Soković Bajić, Svetlana
AU  - Tucović, Dina
AU  - Mutić, Jelena
AU  - Čakić-Milošević, Maja
AU  - Đurđić, Slađana
AU  - Tolinački, Maja
AU  - Popov Aleksandrov, Aleksandra
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Živković, Milica
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5479
AB  - Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is
correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS)
derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for
the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food
supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty
days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led
to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas
the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on
the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation
(IL-1 , TNF- , and IFN-
) in the duodenum. Moreover, micrographs of the duodenum were in line
with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used
16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the
microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly
favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those
changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting
this toxic environmental pollutant.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats
IS  - 3
VL  - 24
DO  - 10.3390/ijms24032845
SP  - 2845
ER  - 

@article{
author = "Brdarić, Emilija and Popović, Dušanka and Soković Bajić, Svetlana and Tucović, Dina and Mutić, Jelena and Čakić-Milošević, Maja and Đurđić, Slađana and Tolinački, Maja and Popov Aleksandrov, Aleksandra and Golić, Nataša and Mirkov, Ivana and Živković, Milica",
year = "2023",
abstract = "Cadmium (Cd) is a highly toxic metal that is distributed worldwide. Exposure to it is
correlated with a vast number of diseases and organism malfunctions. Exopolysaccharides (EPS)
derived from Lactiplantibacillus plantarum BGAN8, EPS-AN8, previously showed great potential for
the in vitro protection of intestinal cells from this metal. Here, we investigated the potential of food
supplemented with EPS-AN8 to protect rats from the hazardous effects of Cd exposure. After thirty
days of exposure to lower (5 ppm) and higher (50 ppm)-Cd doses, the administration of EPS-AN8 led
to decreased Cd content in the kidneys, liver, and blood compared to only Cd-treated groups, whereas
the fecal Cd content was strongly enriched. In addition, EPS-AN8 reversed Cd-provoked effects on
the most significant parameters of oxidative stress (MDA, CAT, GST, and GSH) and inflammation
(IL-1 , TNF- , and IFN-
) in the duodenum. Moreover, micrographs of the duodenum were in line
with these findings. As the gut microbiota has an important role in maintaining homeostasis, we used
16S rRNA amplicon sequencing and investigated the effects of Cd and EPS-AN8 on one part of the
microbiota presented in the duodenum. Although Cd decreased the growth of lactobacilli and mostly
favored the blooming of opportunistic pathogen bacteria, parallel intake of EPS-AN8 reversed those
changes. Therefore, our results imply that EPS-AN8 might be extremely noteworthy in combatting
this toxic environmental pollutant.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats",
number = "3",
volume = "24",
doi = "10.3390/ijms24032845",
pages = "2845"
}

Brdarić, E., Popović, D., Soković Bajić, S., Tucović, D., Mutić, J., Čakić-Milošević, M., Đurđić, S., Tolinački, M., Popov Aleksandrov, A., Golić, N., Mirkov, I.,& Živković, M.. (2023). Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences
Basel: MDPI., 24(3), 2845.
https://doi.org/10.3390/ijms24032845

Brdarić E, Popović D, Soković Bajić S, Tucović D, Mutić J, Čakić-Milošević M, Đurđić S, Tolinački M, Popov Aleksandrov A, Golić N, Mirkov I, Živković M. Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats. in International Journal of Molecular Sciences. 2023;24(3):2845.
doi:10.3390/ijms24032845 .

Brdarić, Emilija, Popović, Dušanka, Soković Bajić, Svetlana, Tucović, Dina, Mutić, Jelena, Čakić-Milošević, Maja, Đurđić, Slađana, Tolinački, Maja, Popov Aleksandrov, Aleksandra, Golić, Nataša, Mirkov, Ivana, Živković, Milica, "Orally Administrated Lactiplantibacillus plantarum BGAN8-Derived EPS-AN8 Ameliorates Cd Hazards in Rats" in International Journal of Molecular Sciences, 24, no. 3 (2023):2845,
https://doi.org/10.3390/ijms24032845 . .

TY  - JOUR
AU  - Bojović, Katarina
AU  - Ignjatović, Đurđica
AU  - Soković Bajić, Svetlana
AU  - Vojnović-Milutinović, Danijela
AU  - Tomić, Mirko
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2020
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32509596
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7248180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3698
AB  - While gut microbiota dysbiosis has been linked with autism, its role in the etiology of other neurodevelopmental disorders (NDD) is largely underexplored. To our knowledge this is the first study to evaluate gut microbiota diversity and composition in 36 children from the Republic of Serbia diagnosed with NDD and 28 healthy children. The results revealed an increased incidence of potentially harmful bacteria, closely related to Clostridium species, in the NDD patient group compared to the Control group: Desulfotomaculum guttoideum (P < 0.01), Intestinibacter bartlettii (P < 0.05), and Romboutsia ilealis (P < 0.001). On the other hand, significantly lower diversity of common commensal bacteria in the NDD group of patients was noticed. Enterococcus faecalis (P < 0.05), Enterococcus gallinarum (P < 0.01), Streptococcus pasteurianus (P < 0.05), Lactobacillus rhamnosus (P < 0.01) and Bifidobacteria sp. were detected in lower numbers of patients or were even absent in some NDD patients. In addition, butyrate-producing bacteria Faecalibacterium prausnitzii (P < 0.01), Butyricicoccus pullicaecorum (P < 0.05), and Eubacterium rectale (P = 0.07) were less frequent in the NDD patient group. In line with that, the levels of fecal short chain fatty acids (SCFAs) were determined. Although significant differences in SCFA levels were not detected between NDD patients and the Control group, a positive correlation was noted between number of rDNA amplicons obtained with universal primers and level of propionic acid, as well as a trend for levels of total SCFAs and butyric acid in the Control group. This correlation is lost in the NDD patient group, indicating that NDD patients' microbiota differs from the microbiota of healthy children in the presence or number of strong SCFA-producing bacteria. According to a range-weighted richness index it was observed that microbial diversity was significantly lower in the NDD patient group. Our study reveals that the intestinal microbiota from NDD patients differs from the microbiota of healthy children. It is hypothesized that early life microbiome might have an impact on GI disturbances and accompanied behavioral problems frequently observed in patients with a broad spectrum of NDD.
PB  - Frontiers Media S.A.
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders.
VL  - 10
DO  - 10.3389/fcimb.2020.00223
SP  - 223
ER  - 

@article{
author = "Bojović, Katarina and Ignjatović, Đurđica and Soković Bajić, Svetlana and Vojnović-Milutinović, Danijela and Tomić, Mirko and Golić, Nataša and Tolinački, Maja",
year = "2020",
abstract = "While gut microbiota dysbiosis has been linked with autism, its role in the etiology of other neurodevelopmental disorders (NDD) is largely underexplored. To our knowledge this is the first study to evaluate gut microbiota diversity and composition in 36 children from the Republic of Serbia diagnosed with NDD and 28 healthy children. The results revealed an increased incidence of potentially harmful bacteria, closely related to Clostridium species, in the NDD patient group compared to the Control group: Desulfotomaculum guttoideum (P < 0.01), Intestinibacter bartlettii (P < 0.05), and Romboutsia ilealis (P < 0.001). On the other hand, significantly lower diversity of common commensal bacteria in the NDD group of patients was noticed. Enterococcus faecalis (P < 0.05), Enterococcus gallinarum (P < 0.01), Streptococcus pasteurianus (P < 0.05), Lactobacillus rhamnosus (P < 0.01) and Bifidobacteria sp. were detected in lower numbers of patients or were even absent in some NDD patients. In addition, butyrate-producing bacteria Faecalibacterium prausnitzii (P < 0.01), Butyricicoccus pullicaecorum (P < 0.05), and Eubacterium rectale (P = 0.07) were less frequent in the NDD patient group. In line with that, the levels of fecal short chain fatty acids (SCFAs) were determined. Although significant differences in SCFA levels were not detected between NDD patients and the Control group, a positive correlation was noted between number of rDNA amplicons obtained with universal primers and level of propionic acid, as well as a trend for levels of total SCFAs and butyric acid in the Control group. This correlation is lost in the NDD patient group, indicating that NDD patients' microbiota differs from the microbiota of healthy children in the presence or number of strong SCFA-producing bacteria. According to a range-weighted richness index it was observed that microbial diversity was significantly lower in the NDD patient group. Our study reveals that the intestinal microbiota from NDD patients differs from the microbiota of healthy children. It is hypothesized that early life microbiome might have an impact on GI disturbances and accompanied behavioral problems frequently observed in patients with a broad spectrum of NDD.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders.",
volume = "10",
doi = "10.3389/fcimb.2020.00223",
pages = "223"
}

Bojović, K., Ignjatović, Đ., Soković Bajić, S., Vojnović-Milutinović, D., Tomić, M., Golić, N.,& Tolinački, M.. (2020). Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders.. in Frontiers in Cellular and Infection Microbiology
Frontiers Media S.A.., 10, 223.
https://doi.org/10.3389/fcimb.2020.00223

Bojović K, Ignjatović Đ, Soković Bajić S, Vojnović-Milutinović D, Tomić M, Golić N, Tolinački M. Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders.. in Frontiers in Cellular and Infection Microbiology. 2020;10:223.
doi:10.3389/fcimb.2020.00223 .

Bojović, Katarina, Ignjatović, Đurđica, Soković Bajić, Svetlana, Vojnović-Milutinović, Danijela, Tomić, Mirko, Golić, Nataša, Tolinački, Maja, "Gut Microbiota Dysbiosis Associated With Altered Production of Short Chain Fatty Acids in Children With Neurodevelopmental Disorders." in Frontiers in Cellular and Infection Microbiology, 10 (2020):223,
https://doi.org/10.3389/fcimb.2020.00223 . .

TY  - CONF
AU  - Živković, Milica
AU  - Soković Bajić, Svetlana
AU  - Tolinački, Maja
AU  - Brdarić, Emilija
AU  - Đokić, Jelena
AU  - Popović, Nikola
AU  - Rajić, Jovana
AU  - Đorđević, Marija
AU  - Golić, Nataša
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5690
AB  - Objective
Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment
of diabetic rats showed decreased hyperglycemia and
ameliorating effect on diabetes-associated damage of liver and kidneys.
Hence, the aim of this study was to reveal the effects of BGCG11 probiotic
on gut microbiota composition and monitoring the insulin production in
pancreatic islets in diabetic rats.
Methods
Experiments were performed on albino Wistar rats divided into four groups:
ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes;
P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were
orally administered with BGCG11, one week before (P/D/P) and after the
STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from
all fecal samples and rDNA amplicons were analyzed by DGGE and 16S
rDNA genes sequencing. For immunohistochemical analysis, slides were
stained with anti-insulin antibody and secondary antibody coupled with
horseradish peroxidase.
Results
The results revealed the higher diversity of gut microbiota in D/P group
comparing to D group, as well as the higher prevalence of Flintibacter
butyricus (the major butyric producer), Acetatifactor muris (present in
obese mouse) and Eisenbergiella massiliensis (found in obese woman),
while the lipolytic bacterium Aestuariispira insulae was more prevalent in
diabetic rats. In both, P/D/P and D/P group, increased number of positive
immunoreactions of β-cells for anti-insulin antibodies was displayed in
compare to D group with islet atrophy.
Conclusions
The results of this study suggest that the positive effect of BGCG11 on
STZ-induced diabetes in rats could be annotated to its protective role on
the integrity of fecal microbiota.
PB  - Wolters Kluwer Health
C3  - 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
T1  - The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11
DO  - 10.1097/MCG.0000000000001292
SP  - 103
ER  - 

@conference{
author = "Živković, Milica and Soković Bajić, Svetlana and Tolinački, Maja and Brdarić, Emilija and Đokić, Jelena and Popović, Nikola and Rajić, Jovana and Đorđević, Marija and Golić, Nataša",
year = "2019",
abstract = "Objective
Our previous studies with Lactobacillus paraplantarum BGCG11 probiotictreatment
of diabetic rats showed decreased hyperglycemia and
ameliorating effect on diabetes-associated damage of liver and kidneys.
Hence, the aim of this study was to reveal the effects of BGCG11 probiotic
on gut microbiota composition and monitoring the insulin production in
pancreatic islets in diabetic rats.
Methods
Experiments were performed on albino Wistar rats divided into four groups:
ND – non-diabetic control, D – streptozotocin (STZ) induced diabetes;
P/D/P – BGCG11 pretreatment; D/P – BGCG11 treatment. The rats were
orally administered with BGCG11, one week before (P/D/P) and after the
STZ injection, for four weeks (P/D/P and D/P). Total DNA was isolated from
all fecal samples and rDNA amplicons were analyzed by DGGE and 16S
rDNA genes sequencing. For immunohistochemical analysis, slides were
stained with anti-insulin antibody and secondary antibody coupled with
horseradish peroxidase.
Results
The results revealed the higher diversity of gut microbiota in D/P group
comparing to D group, as well as the higher prevalence of Flintibacter
butyricus (the major butyric producer), Acetatifactor muris (present in
obese mouse) and Eisenbergiella massiliensis (found in obese woman),
while the lipolytic bacterium Aestuariispira insulae was more prevalent in
diabetic rats. In both, P/D/P and D/P group, increased number of positive
immunoreactions of β-cells for anti-insulin antibodies was displayed in
compare to D group with islet atrophy.
Conclusions
The results of this study suggest that the positive effect of BGCG11 on
STZ-induced diabetes in rats could be annotated to its protective role on
the integrity of fecal microbiota.",
publisher = "Wolters Kluwer Health",
journal = "10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy",
title = "The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11",
doi = "10.1097/MCG.0000000000001292",
pages = "103"
}

Živković, M., Soković Bajić, S., Tolinački, M., Brdarić, E., Đokić, J., Popović, N., Rajić, J., Đorđević, M.,& Golić, N.. (2019). The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
Wolters Kluwer Health., 103.
https://doi.org/10.1097/MCG.0000000000001292

Živković M, Soković Bajić S, Tolinački M, Brdarić E, Đokić J, Popović N, Rajić J, Đorđević M, Golić N. The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy. 2019;:103.
doi:10.1097/MCG.0000000000001292 .

Živković, Milica, Soković Bajić, Svetlana, Tolinački, Maja, Brdarić, Emilija, Đokić, Jelena, Popović, Nikola, Rajić, Jovana, Đorđević, Marija, Golić, Nataša, "The analysis of fecal microbiota and insulin production in diabetic rats after oral administration of probiotic Lactobacillus paraplantarum BGCG11" in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy (2019):103,
https://doi.org/10.1097/MCG.0000000000001292 . .

TY  - CONF
AU  - Ignjatović, Đurđica
AU  - Soković-Bajić, Svetlana
AU  - Bojović, Katarina
AU  - Vojnović-Milutinović, Danijela
AU  - Tomić, Mirko
AU  - Golić, Nataša
AU  - Tolinački, Maja
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5431
AB  - Objective: Clinical reports indicated increased incidence of gastrointestinal (GI) disturbances and dysbiosis in children with neurodevelopmental disorders (NDD) as well as acceleration of the recovery after the dysbiosis treatment. The aim of this study was to evaluate gut microbiota diversity and to identify bacterial strains which incidences were different between patients’ and control group. This is the first study evaluating gut microbiota diversity and composition in children with NDD.
Conclusions: Our study reveals that the intestinal microbiota from children with NDDs differs from the microbiota of healthy children. Similarity of microbiota composition in patient groups with similar neurological and behavioral symptoms indicate that intestinal microbiota might have role in pathophysiology of NDD. Supplementation with several health promoting strains, could be safe adjuvant therapy in treatment of NDD accompanied with GI disturbances.
PB  - Wolters Kluwer Health
C3  - 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
T1  - Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders
VL  - 54
DO  - 10.1097/MCG.0000000000001292
ER  - 

@conference{
author = "Ignjatović, Đurđica and Soković-Bajić, Svetlana and Bojović, Katarina and Vojnović-Milutinović, Danijela and Tomić, Mirko and Golić, Nataša and Tolinački, Maja",
year = "2019",
abstract = "Objective: Clinical reports indicated increased incidence of gastrointestinal (GI) disturbances and dysbiosis in children with neurodevelopmental disorders (NDD) as well as acceleration of the recovery after the dysbiosis treatment. The aim of this study was to evaluate gut microbiota diversity and to identify bacterial strains which incidences were different between patients’ and control group. This is the first study evaluating gut microbiota diversity and composition in children with NDD.
Conclusions: Our study reveals that the intestinal microbiota from children with NDDs differs from the microbiota of healthy children. Similarity of microbiota composition in patient groups with similar neurological and behavioral symptoms indicate that intestinal microbiota might have role in pathophysiology of NDD. Supplementation with several health promoting strains, could be safe adjuvant therapy in treatment of NDD accompanied with GI disturbances.",
publisher = "Wolters Kluwer Health",
journal = "10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy",
title = "Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders",
volume = "54",
doi = "10.1097/MCG.0000000000001292"
}

Ignjatović, Đ., Soković-Bajić, S., Bojović, K., Vojnović-Milutinović, D., Tomić, M., Golić, N.,& Tolinački, M.. (2019). Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy
Wolters Kluwer Health., 54.
https://doi.org/10.1097/MCG.0000000000001292

Ignjatović Đ, Soković-Bajić S, Bojović K, Vojnović-Milutinović D, Tomić M, Golić N, Tolinački M. Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders. in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy. 2019;54.
doi:10.1097/MCG.0000000000001292 .

Ignjatović, Đurđica, Soković-Bajić, Svetlana, Bojović, Katarina, Vojnović-Milutinović, Danijela, Tomić, Mirko, Golić, Nataša, Tolinački, Maja, "Increased incidence of clostridium-like species and lower diversity of common commensal bacteria in gut microbiota of children with neurodevelopmental disorders" in 10th Probiotics, prebiotics and new foods, nutraceuticals and botanicals for nutrition and human and microbiota health and 1st Science; 2019 Sep 8-10; Rome, Italy, 54 (2019),
https://doi.org/10.1097/MCG.0000000000001292 . .

TY  - CONF
AU  - Popović, Dušanka
AU  - Popov Aleksandrov, Aleksandra
AU  - Tucović, Dina
AU  - Kulaš, Jelena
AU  - Zeljković, Milica
AU  - Terzić-Vidojević, Amarela
AU  - Lukić, Jovana
AU  - Golić, Nataša
AU  - Mirkov, Ivana
AU  - Tolinački, Maja
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4793
AB  - The gut microbiome is very important for hosts’ proper essential functions, as microbiota influence both near and distant organs and disruption at any level of this complex relation can be underlying cause for many diseases. Both intestinal bacterial population and their metabolic products are involved in immune system development, activity and maintenance of immune homeostasis. Previous findings revealed differences in immune reactivity in Albino Oxford (AO) and Dark Agouti (DA) rat strains in various diseases models (such as experimental autoimmune encephalomyelitis, rheumatoid arthritis, contact hypersensitivity reaction, pulmonary aspergillosis etc.), and in response to xenobiotics such as cadmium, warfarin, etc.
The aim of present study was to investigate microbial composition of gut (duodenum, jejunum, coecum and colon) and lungs of AO and DA rats using DGGE method. Although similar number of bacterial species were noted in both tissue and lumen of duodenum, bacterial composition differ between these two strains (solely 76.2% and 44.4% species were common in both strains tissue and lumen, respectively), while greater variability was noted in DA rats. Similar results were noted in jejunum. In contrast to duodenum and jejunum, higher number of bacterial species were detected in coecum (content) and colon (tissue and content) of AO rats. Around 50% of detected bacteria were present in both gut segments of both strains. Analysis of DGGE bands obtained from lung tissue revealed similar number of bacteria in both strains, but solely 54.5% were common. Further investigations will be directed to identification of bacterial species and try to connect observed differences in microbial composition with different immune reactivity in AO and DA rats.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade
C3  - Immunology at the Confluence of Multidisciplinary Approaches : abstract book
T1  - Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4793
ER  - 

@conference{
author = "Popović, Dušanka and Popov Aleksandrov, Aleksandra and Tucović, Dina and Kulaš, Jelena and Zeljković, Milica and Terzić-Vidojević, Amarela and Lukić, Jovana and Golić, Nataša and Mirkov, Ivana and Tolinački, Maja",
year = "2019",
abstract = "The gut microbiome is very important for hosts’ proper essential functions, as microbiota influence both near and distant organs and disruption at any level of this complex relation can be underlying cause for many diseases. Both intestinal bacterial population and their metabolic products are involved in immune system development, activity and maintenance of immune homeostasis. Previous findings revealed differences in immune reactivity in Albino Oxford (AO) and Dark Agouti (DA) rat strains in various diseases models (such as experimental autoimmune encephalomyelitis, rheumatoid arthritis, contact hypersensitivity reaction, pulmonary aspergillosis etc.), and in response to xenobiotics such as cadmium, warfarin, etc.
The aim of present study was to investigate microbial composition of gut (duodenum, jejunum, coecum and colon) and lungs of AO and DA rats using DGGE method. Although similar number of bacterial species were noted in both tissue and lumen of duodenum, bacterial composition differ between these two strains (solely 76.2% and 44.4% species were common in both strains tissue and lumen, respectively), while greater variability was noted in DA rats. Similar results were noted in jejunum. In contrast to duodenum and jejunum, higher number of bacterial species were detected in coecum (content) and colon (tissue and content) of AO rats. Around 50% of detected bacteria were present in both gut segments of both strains. Analysis of DGGE bands obtained from lung tissue revealed similar number of bacteria in both strains, but solely 54.5% were common. Further investigations will be directed to identification of bacterial species and try to connect observed differences in microbial composition with different immune reactivity in AO and DA rats.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade",
journal = "Immunology at the Confluence of Multidisciplinary Approaches : abstract book",
title = "Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4793"
}

Popović, D., Popov Aleksandrov, A., Tucović, D., Kulaš, J., Zeljković, M., Terzić-Vidojević, A., Lukić, J., Golić, N., Mirkov, I.,& Tolinački, M.. (2019). Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book
Belgrade: Institute for Biological Research "Siniša Stanković", University of Belgrade., 28.
https://hdl.handle.net/21.15107/rcub_ibiss_4793

Popović D, Popov Aleksandrov A, Tucović D, Kulaš J, Zeljković M, Terzić-Vidojević A, Lukić J, Golić N, Mirkov I, Tolinački M. Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly. in Immunology at the Confluence of Multidisciplinary Approaches : abstract book. 2019;:28.
https://hdl.handle.net/21.15107/rcub_ibiss_4793 .

Popović, Dušanka, Popov Aleksandrov, Aleksandra, Tucović, Dina, Kulaš, Jelena, Zeljković, Milica, Terzić-Vidojević, Amarela, Lukić, Jovana, Golić, Nataša, Mirkov, Ivana, Tolinački, Maja, "Variability of gut and lung microbiota in rat strains known to differ in reactivity to immune stimuly" in Immunology at the Confluence of Multidisciplinary Approaches : abstract book (2019):28,
https://hdl.handle.net/21.15107/rcub_ibiss_4793 .

TY  - JOUR
AU  - Kulaš, Jelena
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Zolotarevski, Lidija
AU  - Glamočlija, Jasmina
AU  - Veljović, Katarina
AU  - Tolinački, Maja
AU  - Golić, Nataša
AU  - Kataranovski, Milena
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0171298518301001?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3165
AB  - Microbiota inhabiting mucosal tissues is involved in maintenance of their immune homeostasis. Growing body of evidence indicate that dysbiosis in gut influence immune responses at distal sites including lungs. There are also reports concerning gut involvement with pulmonary injury/inflammation in settings of respiratory viral and bacterial infections. The impact of infections with other microorganisms on gut homeostasis is not explored. In this study, the rat model of sublethal pulmonary infection with Aspergillus fumigatus was used to investigate the effect of fungal respiratory infection on gut immune-mediated homeostasis. Signs of intestinal damage, intestinal and gut-draining lymphoid tissue cytokine responses and gut bacterial microbiota diversity were examined. Intestinal injury, inflammatory cell infiltration, as well as increased levels of intestinal interferon-γ (IFN-γ) and interleukin-17 (IL-17) (as opposed to unchanged levels of anti-inflammatory cytokine IL-10) during the two-week period depict intestinal inflammation in rats with pulmonary A. fumigatus infection. It could not be ascribed to the fungus as it was not detected in the intestine of infected rats. Increased production of pro-inflammatory cytokines by major gut-draining mesenteric lymph nodes point to these lymphoid organs as places of generation of cytokine-producing cells. No changes in spleen or systemic cytokine responses was observed, showing lack of the effects of pulmonary A. fumigatus infection outside mucosal immune system. Drop of intestinal bacterial microbiota diversity (disappearance of several bacterial bands) was noted early in infection with normalization starting from day seven. From day three, appearance of new bacterial bands (unique to infected individuals, not present in controls) was seen, and some of them are pathogens. Alterations in intestinal bacterial community might have affected intestinal immune tolerance contributing to inflammation. Disruption of gut homeostasis during pulmonary infection might render gastrointestinal tract more susceptible to variety of physiological and pathological stimuli. Data which showed for the first time gut involvement with pulmonary infection with A. fumigatus provide the baseline for future studies of the impact of fungal lung infections to gut homeostasis, particularly in individuals susceptible to these infections.
T2  - Immunobiology
T2  - Immunobiology
T1  - Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis.
IS  - 1
VL  - 224
DO  - 10.1016/j.imbio.2018.10.001
SP  - 116
EP  - 123
ER  - 

@article{
author = "Kulaš, Jelena and Mirkov, Ivana and Tucović, Dina and Zolotarevski, Lidija and Glamočlija, Jasmina and Veljović, Katarina and Tolinački, Maja and Golić, Nataša and Kataranovski, Milena",
year = "2019",
abstract = "Microbiota inhabiting mucosal tissues is involved in maintenance of their immune homeostasis. Growing body of evidence indicate that dysbiosis in gut influence immune responses at distal sites including lungs. There are also reports concerning gut involvement with pulmonary injury/inflammation in settings of respiratory viral and bacterial infections. The impact of infections with other microorganisms on gut homeostasis is not explored. In this study, the rat model of sublethal pulmonary infection with Aspergillus fumigatus was used to investigate the effect of fungal respiratory infection on gut immune-mediated homeostasis. Signs of intestinal damage, intestinal and gut-draining lymphoid tissue cytokine responses and gut bacterial microbiota diversity were examined. Intestinal injury, inflammatory cell infiltration, as well as increased levels of intestinal interferon-γ (IFN-γ) and interleukin-17 (IL-17) (as opposed to unchanged levels of anti-inflammatory cytokine IL-10) during the two-week period depict intestinal inflammation in rats with pulmonary A. fumigatus infection. It could not be ascribed to the fungus as it was not detected in the intestine of infected rats. Increased production of pro-inflammatory cytokines by major gut-draining mesenteric lymph nodes point to these lymphoid organs as places of generation of cytokine-producing cells. No changes in spleen or systemic cytokine responses was observed, showing lack of the effects of pulmonary A. fumigatus infection outside mucosal immune system. Drop of intestinal bacterial microbiota diversity (disappearance of several bacterial bands) was noted early in infection with normalization starting from day seven. From day three, appearance of new bacterial bands (unique to infected individuals, not present in controls) was seen, and some of them are pathogens. Alterations in intestinal bacterial community might have affected intestinal immune tolerance contributing to inflammation. Disruption of gut homeostasis during pulmonary infection might render gastrointestinal tract more susceptible to variety of physiological and pathological stimuli. Data which showed for the first time gut involvement with pulmonary infection with A. fumigatus provide the baseline for future studies of the impact of fungal lung infections to gut homeostasis, particularly in individuals susceptible to these infections.",
journal = "Immunobiology, Immunobiology",
title = "Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis.",
number = "1",
volume = "224",
doi = "10.1016/j.imbio.2018.10.001",
pages = "116-123"
}

Kulaš, J., Mirkov, I., Tucović, D., Zolotarevski, L., Glamočlija, J., Veljović, K., Tolinački, M., Golić, N.,& Kataranovski, M.. (2019). Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis.. in Immunobiology, 224(1), 116-123.
https://doi.org/10.1016/j.imbio.2018.10.001

Kulaš J, Mirkov I, Tucović D, Zolotarevski L, Glamočlija J, Veljović K, Tolinački M, Golić N, Kataranovski M. Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis.. in Immunobiology. 2019;224(1):116-123.
doi:10.1016/j.imbio.2018.10.001 .

Kulaš, Jelena, Mirkov, Ivana, Tucović, Dina, Zolotarevski, Lidija, Glamočlija, Jasmina, Veljović, Katarina, Tolinački, Maja, Golić, Nataša, Kataranovski, Milena, "Pulmonary Aspergillus fumigatus infection in rats affects gastrointestinal homeostasis." in Immunobiology, 224, no. 1 (2019):116-123,
https://doi.org/10.1016/j.imbio.2018.10.001 . .

TY  - JOUR
AU  - Vujičić, Milica
AU  - Saksida, Tamara
AU  - Despotović, Sanja
AU  - Soković Bajić, Svetlana
AU  - Lalić, Ivana
AU  - Koprivica, Ivan
AU  - Mićanović, Dragica
AU  - Golić, Natasa
AU  - Tolinački, Maja
AU  - Stojanović, Ivana D.
PY  - 2018
UR  - http://www.nature.com/articles/s41598-018-24706-3
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC5910418
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3049
AB  - Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability. In these circumstances the diversity and content of gut microbiota in MIF-KO mice was considerably different compared to wild type mice. This change in microbiota was accompanied by an increased intestinal IgA concentration and a higher production of pro-inflammatory cytokines TNF and IFN-γ in mesenteric lymph nodes of MIF-KO mice. The forced changes of microbiota executed by antibiotics prevented the "leakage" of the barrier in MIF-KO mice, probably through up-regulation of occludin expression and normalization of cellular pore diameters. In addition, cytokine secretion was normalized after the treatment with antibiotics. These results suggest that MIF participates in the maintenance of physiological microbiota diversity and immunosurveillance, which in turn enables the proper intestinal barrier function.
T2  - Scientific Reports
T1  - The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.
IS  - 1
VL  - 8
DO  - 10.1038/s41598-018-24706-3
SP  - 6337
ER  - 

@article{
author = "Vujičić, Milica and Saksida, Tamara and Despotović, Sanja and Soković Bajić, Svetlana and Lalić, Ivana and Koprivica, Ivan and Mićanović, Dragica and Golić, Natasa and Tolinački, Maja and Stojanović, Ivana D.",
year = "2018",
abstract = "Macrophage migration inhibitory factor (MIF) is a multifunctional protein that is involved in the development of gut-related inflammation. To investigate the role of MIF in the function of the intestinal barrier, we have explored intestinal permeability and gut-associated immune response in MIF-deficient (MIF-KO) mice. The absence of MIF provoked impairment of tight and adherens epithelial junctions in the colon through the disturbance of E-cadherin, zonula occludens-1, occludin and claudin-2 expression, which lead to the increase of intestinal barrier permeability. In these circumstances the diversity and content of gut microbiota in MIF-KO mice was considerably different compared to wild type mice. This change in microbiota was accompanied by an increased intestinal IgA concentration and a higher production of pro-inflammatory cytokines TNF and IFN-γ in mesenteric lymph nodes of MIF-KO mice. The forced changes of microbiota executed by antibiotics prevented the "leakage" of the barrier in MIF-KO mice, probably through up-regulation of occludin expression and normalization of cellular pore diameters. In addition, cytokine secretion was normalized after the treatment with antibiotics. These results suggest that MIF participates in the maintenance of physiological microbiota diversity and immunosurveillance, which in turn enables the proper intestinal barrier function.",
journal = "Scientific Reports",
title = "The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.",
number = "1",
volume = "8",
doi = "10.1038/s41598-018-24706-3",
pages = "6337"
}

Vujičić, M., Saksida, T., Despotović, S., Soković Bajić, S., Lalić, I., Koprivica, I., Mićanović, D., Golić, N., Tolinački, M.,& Stojanović, I. D.. (2018). The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.. in Scientific Reports, 8(1), 6337.
https://doi.org/10.1038/s41598-018-24706-3

Vujičić M, Saksida T, Despotović S, Soković Bajić S, Lalić I, Koprivica I, Mićanović D, Golić N, Tolinački M, Stojanović ID. The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier.. in Scientific Reports. 2018;8(1):6337.
doi:10.1038/s41598-018-24706-3 .

Vujičić, Milica, Saksida, Tamara, Despotović, Sanja, Soković Bajić, Svetlana, Lalić, Ivana, Koprivica, Ivan, Mićanović, Dragica, Golić, Natasa, Tolinački, Maja, Stojanović, Ivana D., "The Role of Macrophage Migration Inhibitory Factor in the Function of Intestinal Barrier." in Scientific Reports, 8, no. 1 (2018):6337,
https://doi.org/10.1038/s41598-018-24706-3 . .

TY  - JOUR
AU  - Mihailović, Mirjana
AU  - Živković, Milica
AU  - Arambašić Jovanović, Jelena
AU  - Tolinački, Maja
AU  - Đorđević, Marija
AU  - Rajić, Jovana
AU  - Uskoković, Aleksandra
AU  - Dinić, Svetlana
AU  - Grdović, Nevena
AU  - Golić, Nataša
AU  - Vidaković, Melita
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1756464617305546
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2862
AB  - The aim of this study was to assess the effect of the probiotic Lactobacillus paraplantarum BGCG11 on the regulatory pathways that underlie the defense responses of the liver and kidney in diabetic rats. Probiotic-treated diabetic rats exhibited decreased hyperglycemia, glycated hemoglobin, triacylglycerols and a reduction in advanced glycation end products of serum proteins. The probiotic treatment adjusted the redox imbalance in the liver and kidney of diabetic rats, reduced the level of DNA damage, increased the activity of the pro-survival Akt kinase, decreased procaspase 3 degradation and lowered the level of inflammatory mediator C/EBPβ. Administration of probiotic to diabetic rats attenuated fibrotic process activated in the liver and kidneys as judged by the increase in E-cadherin and decreases in α-smooth muscle actin and fibronectin. In summary, the probiotic administration had an ameliorating effect on diabetes-associated disturbed redox homeostasis, inflammation and fibrosis, which underline the development of diabetic complications.
T2  - Journal of Functional Foods
T1  - Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats
IS  - Part A
VL  - 38
DO  - 10.1016/j.jff.2017.09.033
SP  - 427
EP  - 437
ER  - 

@article{
author = "Mihailović, Mirjana and Živković, Milica and Arambašić Jovanović, Jelena and Tolinački, Maja and Đorđević, Marija and Rajić, Jovana and Uskoković, Aleksandra and Dinić, Svetlana and Grdović, Nevena and Golić, Nataša and Vidaković, Melita",
year = "2017",
abstract = "The aim of this study was to assess the effect of the probiotic Lactobacillus paraplantarum BGCG11 on the regulatory pathways that underlie the defense responses of the liver and kidney in diabetic rats. Probiotic-treated diabetic rats exhibited decreased hyperglycemia, glycated hemoglobin, triacylglycerols and a reduction in advanced glycation end products of serum proteins. The probiotic treatment adjusted the redox imbalance in the liver and kidney of diabetic rats, reduced the level of DNA damage, increased the activity of the pro-survival Akt kinase, decreased procaspase 3 degradation and lowered the level of inflammatory mediator C/EBPβ. Administration of probiotic to diabetic rats attenuated fibrotic process activated in the liver and kidneys as judged by the increase in E-cadherin and decreases in α-smooth muscle actin and fibronectin. In summary, the probiotic administration had an ameliorating effect on diabetes-associated disturbed redox homeostasis, inflammation and fibrosis, which underline the development of diabetic complications.",
journal = "Journal of Functional Foods",
title = "Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats",
number = "Part A",
volume = "38",
doi = "10.1016/j.jff.2017.09.033",
pages = "427-437"
}

Mihailović, M., Živković, M., Arambašić Jovanović, J., Tolinački, M., Đorđević, M., Rajić, J., Uskoković, A., Dinić, S., Grdović, N., Golić, N.,& Vidaković, M.. (2017). Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats. in Journal of Functional Foods, 38(Part A), 427-437.
https://doi.org/10.1016/j.jff.2017.09.033

Mihailović M, Živković M, Arambašić Jovanović J, Tolinački M, Đorđević M, Rajić J, Uskoković A, Dinić S, Grdović N, Golić N, Vidaković M. Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats. in Journal of Functional Foods. 2017;38(Part A):427-437.
doi:10.1016/j.jff.2017.09.033 .

Mihailović, Mirjana, Živković, Milica, Arambašić Jovanović, Jelena, Tolinački, Maja, Đorđević, Marija, Rajić, Jovana, Uskoković, Aleksandra, Dinić, Svetlana, Grdović, Nevena, Golić, Nataša, Vidaković, Melita, "Oral administration of probiotic Lactobacillus paraplantarum BGCG11 attenuates diabetes-induced liver and kidney damage in rats" in Journal of Functional Foods, 38, no. Part A (2017):427-437,
https://doi.org/10.1016/j.jff.2017.09.033 . .

TY  - CONF
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Mileusnić, Dina
AU  - Zolotarevski, Lidija
AU  - Tolinački, Maja
AU  - Kataranovski, Dragan
AU  - Brceski, Ilija
AU  - Kataranovski, Milena
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4810
AB  - Conflicting data exist concerning cadmium
effects on immune system depending on the
experimental model, exposure or tissue/activity
examined. In this study immunotoxicity of acute
intraperitoneal and oral cadmium
administration was investigated in rats. The
use of the inflammation-prone inbred Dark
Agouti (DA) and less reactive Albino Oxford
(AO) rats showed differential (immune activityrelated and/or strain-related) effects of
cadmium (1 mg of Cd/kg, i.p.) on spleen
immune responses. A decrease in ConAinduced proliferation (related to altered spleen
cells responsiveness to IL-2) and of IFN-γ
(independently of IL-4 and IL-10) was more
pronounced in DA rats. Increased innate
immunity splenocyte activity (granulocyte
CD11b+ cells, iNOS mRNA and NO
production, myeloperoxidase MPO activity, IL1β mRNA and IL-1β protein product levels)
were observed in both strains (some of them
more pronounced in DA rats), while a decrease
in respiratory burst (dihydrorhodamine/DHR
oxidation) was similar. 30-day oral intake of 5
ppm and 50 ppm of cadmium by DA rats
resulted in reduction of some probiotic bacteria,
villous damage and intestinal inflammation
[(increased levels of High Mobility Group
Box1/HMGB1, antioxidant enzyme (superoxide
dismutase/SOD and catalase/CAT) activity and
proinflammatory cytokine (TNF, IL-1β, IFN-γ,
IL-17) in gut homogenates]. Stimulation of both
adaptive (increased cellularity, proliferation,
IFN-γ and IL-17cytokine responses) as well as
innate immune activity (increases in numbers
of NK cells and M1-like macrophages,
oxidative cell activities, IL-1β) of gut draining
(mesenteric) lymph nodes was associated with
decreased or unchanged antiinflammatory
cytokine (IL-10) cell response. Differential
(immunosuppressive and immunostimulatory)
effects noted in the same tissue (spleen)
should be taken into account when exploring
immunotoxicity of this metal. Stimulation of gut
immune responses imply dietary cadmium as
health risk factor.
PB  - Department of Biomedical Sciences-Histology of the University of Sassari
C3  - II Cadmium Symposium, June 25-27, 2015, Sassari, Italy
T1  - Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats
SP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4810
ER  - 

@conference{
author = "Demenesku, Jelena and Ninkov, Marina and Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Mileusnić, Dina and Zolotarevski, Lidija and Tolinački, Maja and Kataranovski, Dragan and Brceski, Ilija and Kataranovski, Milena",
year = "2015",
abstract = "Conflicting data exist concerning cadmium
effects on immune system depending on the
experimental model, exposure or tissue/activity
examined. In this study immunotoxicity of acute
intraperitoneal and oral cadmium
administration was investigated in rats. The
use of the inflammation-prone inbred Dark
Agouti (DA) and less reactive Albino Oxford
(AO) rats showed differential (immune activityrelated and/or strain-related) effects of
cadmium (1 mg of Cd/kg, i.p.) on spleen
immune responses. A decrease in ConAinduced proliferation (related to altered spleen
cells responsiveness to IL-2) and of IFN-γ
(independently of IL-4 and IL-10) was more
pronounced in DA rats. Increased innate
immunity splenocyte activity (granulocyte
CD11b+ cells, iNOS mRNA and NO
production, myeloperoxidase MPO activity, IL1β mRNA and IL-1β protein product levels)
were observed in both strains (some of them
more pronounced in DA rats), while a decrease
in respiratory burst (dihydrorhodamine/DHR
oxidation) was similar. 30-day oral intake of 5
ppm and 50 ppm of cadmium by DA rats
resulted in reduction of some probiotic bacteria,
villous damage and intestinal inflammation
[(increased levels of High Mobility Group
Box1/HMGB1, antioxidant enzyme (superoxide
dismutase/SOD and catalase/CAT) activity and
proinflammatory cytokine (TNF, IL-1β, IFN-γ,
IL-17) in gut homogenates]. Stimulation of both
adaptive (increased cellularity, proliferation,
IFN-γ and IL-17cytokine responses) as well as
innate immune activity (increases in numbers
of NK cells and M1-like macrophages,
oxidative cell activities, IL-1β) of gut draining
(mesenteric) lymph nodes was associated with
decreased or unchanged antiinflammatory
cytokine (IL-10) cell response. Differential
(immunosuppressive and immunostimulatory)
effects noted in the same tissue (spleen)
should be taken into account when exploring
immunotoxicity of this metal. Stimulation of gut
immune responses imply dietary cadmium as
health risk factor.",
publisher = "Department of Biomedical Sciences-Histology of the University of Sassari",
journal = "II Cadmium Symposium, June 25-27, 2015, Sassari, Italy",
title = "Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats",
pages = "31",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4810"
}

Demenesku, J., Ninkov, M., Popov Aleksandrov, A., Mirkov, I., Mileusnić, D., Zolotarevski, L., Tolinački, M., Kataranovski, D., Brceski, I.,& Kataranovski, M.. (2015). Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats. in II Cadmium Symposium, June 25-27, 2015, Sassari, Italy
Department of Biomedical Sciences-Histology of the University of Sassari., 31.
https://hdl.handle.net/21.15107/rcub_ibiss_4810

Demenesku J, Ninkov M, Popov Aleksandrov A, Mirkov I, Mileusnić D, Zolotarevski L, Tolinački M, Kataranovski D, Brceski I, Kataranovski M. Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats. in II Cadmium Symposium, June 25-27, 2015, Sassari, Italy. 2015;:31.
https://hdl.handle.net/21.15107/rcub_ibiss_4810 .

Demenesku, Jelena, Ninkov, Marina, Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Mileusnić, Dina, Zolotarevski, Lidija, Tolinački, Maja, Kataranovski, Dragan, Brceski, Ilija, Kataranovski, Milena, "Immunotoxicology of cadmium: Insight from acute intraperitoneal and intermediate period of oral exposure of rats" in II Cadmium Symposium, June 25-27, 2015, Sassari, Italy (2015):31,
https://hdl.handle.net/21.15107/rcub_ibiss_4810 .