TY  - JOUR
AU  - Vidović, Nevena
AU  - Ranković, Slavica
AU  - Oreščanin Dušić, Zorana
AU  - Debeljak-Martačić, Jasmina
AU  - Popović, Tamara
AU  - Tomić, Mirko
AU  - Glibetić, Marija
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0042-84501800122V
UR  - https://radar.ibiss.bg.ac.rs/123456789/3878
AB  - Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ± 0.73 U/mg) and CAT (147.25 ± 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ± 0.11 U/mg; CAT: 99.37 ± 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ± 0.52 U/mg; CAT: 104.18 ± 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ± 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ± 0.08 nmoL/mg; the fish-based diet group: 1.13 ± 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one’s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants
PB  - National Library of Serbia
T2  - Vojnosanitetski pregled
T1  - The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study
IS  - 6
VL  - 77
DO  - 10.2298/VSP180326122V
SP  - 641
EP  - 646
ER  - 

@article{
author = "Vidović, Nevena and Ranković, Slavica and Oreščanin Dušić, Zorana and Debeljak-Martačić, Jasmina and Popović, Tamara and Tomić, Mirko and Glibetić, Marija",
year = "2020",
abstract = "Background/Aim. Recently, there has been an increased interest in novel dietary antioxidants, including omega-3 fatty acids and bioactive proteins present in milk. The aim of this study was to examine potential antioxidant effects of four-weeks long fish-based and milk-based diets in female Wistar rats. Methods. Four-months old rats were divided into three groups receiving either: control diet, diet enriched with fish meal, or diet enriched with milk. The activities of antioxidant enzymes: glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase (CAT), and concentration of thiobarbituric acid reactive substances (TBARS) were determined in liver homogenates obtained at the end of the treatment period. Results. Statistically significant higher activities of GPx (3.52 ± 0.73 U/mg) and CAT (147.25 ± 15.93 U/mg) were detected in rats fed with fishbased meal in comparison with both the control (GPx: 1.93 ± 0.11 U/mg; CAT: 99.37 ± 10.03 U/mg) and the group fed with milk-based diet (GPx: 1.72 ± 0.52 U/mg; CAT: 104.18 ± 37.49 U/mg). Despite somewhat lower concentration of TBARS in the milk-treated group (0.88 ± 0.23 nmoL/mg), no significant differences were detected in comparison with other groups (the control group: 1.00 ± 0.08 nmoL/mg; the fish-based diet group: 1.13 ± 0.15 nmoL/mg). Conclusion. Diet enriched with fish could improve one’s oxidative status by enhancing activities of antioxidant enzymes in the liver tissue. On the contrary, we failed to obtain results suggesting that milk could serve as a source of dietary antioxidants",
publisher = "National Library of Serbia",
journal = "Vojnosanitetski pregled",
title = "The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study",
number = "6",
volume = "77",
doi = "10.2298/VSP180326122V",
pages = "641-646"
}

Vidović, N., Ranković, S., Oreščanin Dušić, Z., Debeljak-Martačić, J., Popović, T., Tomić, M.,& Glibetić, M.. (2020). The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study. in Vojnosanitetski pregled
National Library of Serbia., 77(6), 641-646.
https://doi.org/10.2298/VSP180326122V

Vidović N, Ranković S, Oreščanin Dušić Z, Debeljak-Martačić J, Popović T, Tomić M, Glibetić M. The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study. in Vojnosanitetski pregled. 2020;77(6):641-646.
doi:10.2298/VSP180326122V .

Vidović, Nevena, Ranković, Slavica, Oreščanin Dušić, Zorana, Debeljak-Martačić, Jasmina, Popović, Tamara, Tomić, Mirko, Glibetić, Marija, "The effects of fish-based and milk-based diets on liver tissue antioxidant enzymes and lipid peroxidation in female Wistar rats: A pilot study" in Vojnosanitetski pregled, 77, no. 6 (2020):641-646,
https://doi.org/10.2298/VSP180326122V . .

TY  - JOUR
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Petrović, Snježana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Glibetić, Maria
PY  - 2017
UR  - http://lipidworld.biomedcentral.com/articles/10.1186/s12944-017-0483-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2768
AB  - Background: Dietary intake influence changes in fatty acids (FA) profiles in liver which plays a central role in fatty acid metabolism, triacylglycerol synthesis and energy homeostasis. We investigated the effects of 4-weeks treatment with milk- and fish-based diet, on plasma biochemical parameters and FA composition of liver phospholipids (PL) in rats of both sexes. Methods: Adult, 4 months old, Wistar rats of both sexes, were fed with different types of diets: standard, milk-based and fish-based, during 4 weeks. Analytical characterization of different foods was done. Biochemical parameters in plasma were determined. Fatty acid composition was analyzed by gas-chromatography. Statistical significance of FA levels was tested with two-way analysis of variance (ANOVA) using the sex of animals and treatment (type of diet) as factors on logarithmic or trigonometric transformed data. Results: Our results showed that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Initially present sex differences appear to be dietary modulated. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), and effects were gender specific. Milk-based diet lowered SFA and elevated MUFA in males and increased PUFA in females vs. standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males. Fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio vs. standard and milk-based diet in females. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males. Conclusion: Gender related variations in FA composition of rat liver PL were observed, and results have shown that those initial differences could be significantly modulated by the type of diet. Furthermore, the modulatory effects of milk- and fish-based diets on liver phospholipids FA profiles appeared to be sex-specific.
T2  - Lipids in Health and Disease
T1  - Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes
IS  - 1
VL  - 16
DO  - 10.1186/s12944-017-0483-9
SP  - 94
EP  - 94
ER  - 

@article{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Petrović, Snježana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Glibetić, Maria",
year = "2017",
abstract = "Background: Dietary intake influence changes in fatty acids (FA) profiles in liver which plays a central role in fatty acid metabolism, triacylglycerol synthesis and energy homeostasis. We investigated the effects of 4-weeks treatment with milk- and fish-based diet, on plasma biochemical parameters and FA composition of liver phospholipids (PL) in rats of both sexes. Methods: Adult, 4 months old, Wistar rats of both sexes, were fed with different types of diets: standard, milk-based and fish-based, during 4 weeks. Analytical characterization of different foods was done. Biochemical parameters in plasma were determined. Fatty acid composition was analyzed by gas-chromatography. Statistical significance of FA levels was tested with two-way analysis of variance (ANOVA) using the sex of animals and treatment (type of diet) as factors on logarithmic or trigonometric transformed data. Results: Our results showed that both, milk- and fish-based diet, changed the composition and ratio of rat liver phospholipids FA, in gender-specific manner. Initially present sex differences appear to be dietary modulated. Although, applied diets changed the ratio of total saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA), and effects were gender specific. Milk-based diet lowered SFA and elevated MUFA in males and increased PUFA in females vs. standard diet. The same diet decreased n-3, increased n-6 and n-6/n-3 ratio in males. Fish-based diet increased n-3, decreased n-6 and n-6/n-3 ratio vs. standard and milk-based diet in females. However, the ratio of individual FA in liver PL was also dietary-influenced, but with gender specific manner. While in females fish-based diet decreased AA (arachidonic acid) increased level of EPA (eicosapentaenoic acid), DPA (docosapentaenoic acid) and DHA (docosahexaenoic acid), the same diet elevated only DHA levels in males. Conclusion: Gender related variations in FA composition of rat liver PL were observed, and results have shown that those initial differences could be significantly modulated by the type of diet. Furthermore, the modulatory effects of milk- and fish-based diets on liver phospholipids FA profiles appeared to be sex-specific.",
journal = "Lipids in Health and Disease",
title = "Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes",
number = "1",
volume = "16",
doi = "10.1186/s12944-017-0483-9",
pages = "94-94"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Petrović, S., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Glibetić, M.. (2017). Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes. in Lipids in Health and Disease, 16(1), 94-94.
https://doi.org/10.1186/s12944-017-0483-9

Ranković S, Popović T, Debeljak Martačić J, Petrović S, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes. in Lipids in Health and Disease. 2017;16(1):94-94.
doi:10.1186/s12944-017-0483-9 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Petrović, Snježana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Glibetić, Maria, "Liver phospholipids fatty acids composition in response to different types of diets in rats of both sexes" in Lipids in Health and Disease, 16, no. 1 (2017):94-94,
https://doi.org/10.1186/s12944-017-0483-9 . .

TY  - JOUR
AU  - Zec, Manja
AU  - Debeljak Martačić, Jasmina
AU  - Ranković, Slavica
AU  - Pokimica, Biljana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Glibetić, Maria
AU  - Popović, Tamara
PY  - 2017
UR  - http://www.degruyter.com/view/j/acve.2017.67.issue-1/acve-2017-0010/acve-2017-0010.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2731
AB  - A nutritional placebo-controlled study was performed in Wistar rats in order to investigate the effects of 5-weeks aronia juice consumption towards fatty acid (FA) composition of phospholipids in the plasma and liver, as well as plasma glucose (Glu) and cholesterol levels. The animals were divided into 3 groups of 8 animals each, and randomized to receive either the full polyphenol dose of Aronia melanocarpa juice (AMJ), 4 times less polyphenol dose (1/4-AMJ) or polyphenol-lacking placebo beverage (PLB). Each group of 8 male adult Wistar rats received the liquid ad libitum. AMJ decreased the levels of low-density lipoprotein (LDL) (P < 0.05) vs. PLB. AMJ increased dihomo-γ-linoleic acid (DGLA, 20:3n-6) (P < 0.05) and decreased arachidonic acid content (AA, 20:4n-6) (P < 0.05) vs. PLB in liver phospholipids. AMJ significantly increased monounsaturated fatty acids (MUFA) levels both in the liver (P < 0.05) and plasma (P < 0.05). Both aronia juice doses elevated the levels of beneficial n-3 polyunsaturated fatty acids (PUFA) in the plasma and liver. There was a dose-dependent, significant increase (P < 0.001) in cis-vaccenic acid (VA, 18:1n-7) in phospholipids in the plasma and liver. Our results indicate favorable effects of aronia juice intake on lipid parameters in Wistar rats. These findings suggest the potential of aronia dietary intake in cardiometabolic diseases primary prevention strategies in the human population.
T2  - Acta Veterinaria
T1  - Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats
IS  - 1
VL  - 67
DO  - 10.1515/acve-2017-0010
SP  - 107
EP  - 120
ER  - 

@article{
author = "Zec, Manja and Debeljak Martačić, Jasmina and Ranković, Slavica and Pokimica, Biljana and Tomić, Mirko and Ignjatović, Đurđica and Glibetić, Maria and Popović, Tamara",
year = "2017",
abstract = "A nutritional placebo-controlled study was performed in Wistar rats in order to investigate the effects of 5-weeks aronia juice consumption towards fatty acid (FA) composition of phospholipids in the plasma and liver, as well as plasma glucose (Glu) and cholesterol levels. The animals were divided into 3 groups of 8 animals each, and randomized to receive either the full polyphenol dose of Aronia melanocarpa juice (AMJ), 4 times less polyphenol dose (1/4-AMJ) or polyphenol-lacking placebo beverage (PLB). Each group of 8 male adult Wistar rats received the liquid ad libitum. AMJ decreased the levels of low-density lipoprotein (LDL) (P < 0.05) vs. PLB. AMJ increased dihomo-γ-linoleic acid (DGLA, 20:3n-6) (P < 0.05) and decreased arachidonic acid content (AA, 20:4n-6) (P < 0.05) vs. PLB in liver phospholipids. AMJ significantly increased monounsaturated fatty acids (MUFA) levels both in the liver (P < 0.05) and plasma (P < 0.05). Both aronia juice doses elevated the levels of beneficial n-3 polyunsaturated fatty acids (PUFA) in the plasma and liver. There was a dose-dependent, significant increase (P < 0.001) in cis-vaccenic acid (VA, 18:1n-7) in phospholipids in the plasma and liver. Our results indicate favorable effects of aronia juice intake on lipid parameters in Wistar rats. These findings suggest the potential of aronia dietary intake in cardiometabolic diseases primary prevention strategies in the human population.",
journal = "Acta Veterinaria",
title = "Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats",
number = "1",
volume = "67",
doi = "10.1515/acve-2017-0010",
pages = "107-120"
}

Zec, M., Debeljak Martačić, J., Ranković, S., Pokimica, B., Tomić, M., Ignjatović, Đ., Glibetić, M.,& Popović, T.. (2017). Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats. in Acta Veterinaria, 67(1), 107-120.
https://doi.org/10.1515/acve-2017-0010

Zec M, Debeljak Martačić J, Ranković S, Pokimica B, Tomić M, Ignjatović Đ, Glibetić M, Popović T. Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats. in Acta Veterinaria. 2017;67(1):107-120.
doi:10.1515/acve-2017-0010 .

Zec, Manja, Debeljak Martačić, Jasmina, Ranković, Slavica, Pokimica, Biljana, Tomić, Mirko, Ignjatović, Đurđica, Glibetić, Maria, Popović, Tamara, "Effects of Aronia melanocarpa juice on plasma and liver phospholipid fatty acid composition in Wistar rats" in Acta Veterinaria, 67, no. 1 (2017):107-120,
https://doi.org/10.1515/acve-2017-0010 . .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Petrović Oggiano, Gordana
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Miler, Marko
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6347
AB  - Cardiovascular diseases (CVD) are still leading cause of death worldwide. It has been shown that folic acid deficiency can modify polyunsaturated fatty acid (PUFA) metabolism and thus could contribute to the development of (CVD). The contents of (n-6) and (n-3) long-chain PUFA in cell membranes, are important determinants of the biophysical properties of those membranes. The long-chain n-3 PUFAs have been shown to have beneficial effects in delaying the development of various diseases. The aim of our study was to examine the effects of food enriched with fish flour and/or food enriched with fish flour + folic acid on fatty acid composition of liver lipids in 4 weeks treatment. Fe- male Wistar rats were randomly assigned into 3 experimental groups (n=10, 280g±5). Control group received standard food (Veterinarski zavod, Subotica). Both treated groups received a food enriched with fish flour, while in second treated group folic acid was added. After sacrificing, part of the liver was frozen at -80 °C. The phospholipids fatty acid composition of liver was determined by GC using Shimadzu GC. Our results showed that food enriched with fish flour significantly increased: eicosatrienoic (20:3), eicosapentaenoic acid (20:5), docosapentaenoic acid (22:5), docosahexaenoic acid (22:6), n-3 and decreased arachidonic acid, n-6 and n-6/n-3 ratio of liver phospholipids concentrations vs. control. Food enriched with folic acid increased (20:3), (20:5), (22:5), (22:6), PUFA, n-3 and decreased MUFA and palmitic acid (16:0) and n-6/n-3 ratio vs. control. Both type of food significantly decreased concentration of and linoleic (18:2) and vascenic acid (18:1, n-7).
PB  - Belgrade: Serbian Physiology Society
C3  - Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia
T1  - Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats
SP  - 139
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6347
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Petrović Oggiano, Gordana and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Miler, Marko and Glibetić, Marija",
year = "2015",
abstract = "Cardiovascular diseases (CVD) are still leading cause of death worldwide. It has been shown that folic acid deficiency can modify polyunsaturated fatty acid (PUFA) metabolism and thus could contribute to the development of (CVD). The contents of (n-6) and (n-3) long-chain PUFA in cell membranes, are important determinants of the biophysical properties of those membranes. The long-chain n-3 PUFAs have been shown to have beneficial effects in delaying the development of various diseases. The aim of our study was to examine the effects of food enriched with fish flour and/or food enriched with fish flour + folic acid on fatty acid composition of liver lipids in 4 weeks treatment. Fe- male Wistar rats were randomly assigned into 3 experimental groups (n=10, 280g±5). Control group received standard food (Veterinarski zavod, Subotica). Both treated groups received a food enriched with fish flour, while in second treated group folic acid was added. After sacrificing, part of the liver was frozen at -80 °C. The phospholipids fatty acid composition of liver was determined by GC using Shimadzu GC. Our results showed that food enriched with fish flour significantly increased: eicosatrienoic (20:3), eicosapentaenoic acid (20:5), docosapentaenoic acid (22:5), docosahexaenoic acid (22:6), n-3 and decreased arachidonic acid, n-6 and n-6/n-3 ratio of liver phospholipids concentrations vs. control. Food enriched with folic acid increased (20:3), (20:5), (22:5), (22:6), PUFA, n-3 and decreased MUFA and palmitic acid (16:0) and n-6/n-3 ratio vs. control. Both type of food significantly decreased concentration of and linoleic (18:2) and vascenic acid (18:1, n-7).",
publisher = "Belgrade: Serbian Physiology Society",
journal = "Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia",
title = "Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats",
pages = "139-139",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6347"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Petrović Oggiano, G., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G., Miler, M.,& Glibetić, M.. (2015). Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats. in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia
Belgrade: Serbian Physiology Society., 139-139.
https://hdl.handle.net/21.15107/rcub_ibiss_6347

Ranković S, Popović T, Debeljak Martačić J, Petrović Oggiano G, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Miler M, Glibetić M. Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats. in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia. 2015;:139-139.
https://hdl.handle.net/21.15107/rcub_ibiss_6347 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Petrović Oggiano, Gordana, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Miler, Marko, Glibetić, Marija, "Effect of omega 3 and folic acid on phospholipids fatty acid composition of liver rats" in Abstract book with final program: Heart Diseases: How New Research May Lead to New Treatments: 2nd European Section Meeting of the International Academy of Cardiovascular Sciences; 2015 Oct 8-10; Belgrade, Serbia (2015):139-139,
https://hdl.handle.net/21.15107/rcub_ibiss_6347 .

TY  - CONF
AU  - Ranković, Slavica
AU  - Popović, Tamara
AU  - Debeljak Martačić, Jasmina
AU  - Tomić, Mirko
AU  - Ignjatović, Đurđica
AU  - Tovilović-Kovačević, Gordana
AU  - Glibetić, Marija
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6348
AB  - Dietary intake of different food influences fatty acid composition of lipid fractions, especially phospholipids as the main constituents of cell membranes. The polyunsaturated fatty acids (PUFA) intake is more and more associated with the prevention and development of chronic diseases with an inflammatory component. The liver has an important role in the synthesis and metabolism of phospholipids. Liver phospholipids build the structure of the hepatocyte membrane, take part in metabolic activities and repairing after metabolic disturbances. Our aim was to investigate the effects of food enriched with fish flour and/or food enriched with milk powder on fatty acid composition of liver lipids in rats treated for 4 weeks. Male Wistar rats were randomly assigned into three experimental groups (n=10, 375g±5). The control group recieved standard food (Veterinarski zavod, Subotica). Group II as fed a diet with fish flour and III received a diet with milk powder. The animals were sacrificed by decapitation and part of the liver was frozen at –80 °C. The fatty acid composition of liver phospholipids was determined by Gas Chromatography (Shimadzu GC). Our results showed that food enriched with fish flour significantly increased liver phospholipid concentrations of docosahexaenoic acid (22:6), PUFA and n-3 and decreased n-6/n-3 ratio compared to control. Food enriched with milk powder increased arachidonic acid (20:4), MUFA, PUFA, n-6 and n-6/n-3 ratio while decreased eicosapentaenoic acid (20:5), (22:6) and n-3 compared to control. Fish oil/powder is a well known antioxidant. Food enriched with fish flour is more efficient due to decrease of n-6/n-3 ratio (as a risk factor) and our future examinations will address it.
PB  - Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology
C3  - Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia
T1  - Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6348
ER  - 

@conference{
author = "Ranković, Slavica and Popović, Tamara and Debeljak Martačić, Jasmina and Tomić, Mirko and Ignjatović, Đurđica and Tovilović-Kovačević, Gordana and Glibetić, Marija",
year = "2015",
abstract = "Dietary intake of different food influences fatty acid composition of lipid fractions, especially phospholipids as the main constituents of cell membranes. The polyunsaturated fatty acids (PUFA) intake is more and more associated with the prevention and development of chronic diseases with an inflammatory component. The liver has an important role in the synthesis and metabolism of phospholipids. Liver phospholipids build the structure of the hepatocyte membrane, take part in metabolic activities and repairing after metabolic disturbances. Our aim was to investigate the effects of food enriched with fish flour and/or food enriched with milk powder on fatty acid composition of liver lipids in rats treated for 4 weeks. Male Wistar rats were randomly assigned into three experimental groups (n=10, 375g±5). The control group recieved standard food (Veterinarski zavod, Subotica). Group II as fed a diet with fish flour and III received a diet with milk powder. The animals were sacrificed by decapitation and part of the liver was frozen at –80 °C. The fatty acid composition of liver phospholipids was determined by Gas Chromatography (Shimadzu GC). Our results showed that food enriched with fish flour significantly increased liver phospholipid concentrations of docosahexaenoic acid (22:6), PUFA and n-3 and decreased n-6/n-3 ratio compared to control. Food enriched with milk powder increased arachidonic acid (20:4), MUFA, PUFA, n-6 and n-6/n-3 ratio while decreased eicosapentaenoic acid (20:5), (22:6) and n-3 compared to control. Fish oil/powder is a well known antioxidant. Food enriched with fish flour is more efficient due to decrease of n-6/n-3 ratio (as a risk factor) and our future examinations will address it.",
publisher = "Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology",
journal = "Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia",
title = "Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6348"
}

Ranković, S., Popović, T., Debeljak Martačić, J., Tomić, M., Ignjatović, Đ., Tovilović-Kovačević, G.,& Glibetić, M.. (2015). Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats. in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia
Belgrade: Serbian Society for Mitochondrial and Free-Radical Physiology., 85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6348

Ranković S, Popović T, Debeljak Martačić J, Tomić M, Ignjatović Đ, Tovilović-Kovačević G, Glibetić M. Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats. in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia. 2015;:85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6348 .

Ranković, Slavica, Popović, Tamara, Debeljak Martačić, Jasmina, Tomić, Mirko, Ignjatović, Đurđica, Tovilović-Kovačević, Gordana, Glibetić, Marija, "Food enriched with fish flour decrease n-6/n-3 ratio in liver phospholipids in male Wistar rats" in Book of Abstracts: Third Congress Redox Medicine: Reactive Species Signaling, Analytical Methods, Phytopharmacy, Molecular Mechanisms of Disease - SSMFRP-2015; 2015 Sep 25-26; Belgrade, Serbia (2015):85-85,
https://hdl.handle.net/21.15107/rcub_ibiss_6348 .