TY  - JOUR
AU  - Constantin, Stephanie
AU  - Bjelobaba, Ivana
AU  - Stojilković, Stanko S.
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1471489222001011
UR  - http://www.ncbi.nlm.nih.gov/pubmed/35994915
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9509429
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5105
AB  - Pituitary gonadotrophs play a key role in reproductive functions by secreting luteinizing hormone (LH) and follicle-stimulating hormone (FSH). The LH secretory activity of gonadotroph is controlled by hypothalamic gonadotropin-releasing hormone (GnRH) via GnRH receptors and is accompanied by only minor effects on high basal Lhb gene expression. The secretory profiles of GnRH and LH are highly synchronized, with the latter reflecting a depletion of prestored LH in secretory vesicles by regulated exocytosis. In contrast, FSH is predominantly released by constitutive exocytosis, and secretory activity reflects the kinetics of Fshb gene expression controlled by GnRH, activin, and inhibin. Here is a review of recent data to improve the understanding of multiple patterns of gonadotroph gene expression and hormone secretion.
PB  - Elsevier B.V.
T2  - Current Opinion in Pharmacology
T1  - Pituitary gonadotroph-specific patterns of gene expression and hormone secretion.
VL  - 66
DO  - 10.1016/j.coph.2022.102274
SP  - 102274
ER  - 

@article{
author = "Constantin, Stephanie and Bjelobaba, Ivana and Stojilković, Stanko S.",
year = "2022",
abstract = "Pituitary gonadotrophs play a key role in reproductive functions by secreting luteinizing hormone (LH) and follicle-stimulating hormone (FSH). The LH secretory activity of gonadotroph is controlled by hypothalamic gonadotropin-releasing hormone (GnRH) via GnRH receptors and is accompanied by only minor effects on high basal Lhb gene expression. The secretory profiles of GnRH and LH are highly synchronized, with the latter reflecting a depletion of prestored LH in secretory vesicles by regulated exocytosis. In contrast, FSH is predominantly released by constitutive exocytosis, and secretory activity reflects the kinetics of Fshb gene expression controlled by GnRH, activin, and inhibin. Here is a review of recent data to improve the understanding of multiple patterns of gonadotroph gene expression and hormone secretion.",
publisher = "Elsevier B.V.",
journal = "Current Opinion in Pharmacology",
title = "Pituitary gonadotroph-specific patterns of gene expression and hormone secretion.",
volume = "66",
doi = "10.1016/j.coph.2022.102274",
pages = "102274"
}

Constantin, S., Bjelobaba, I.,& Stojilković, S. S.. (2022). Pituitary gonadotroph-specific patterns of gene expression and hormone secretion.. in Current Opinion in Pharmacology
Elsevier B.V.., 66, 102274.
https://doi.org/10.1016/j.coph.2022.102274

Constantin S, Bjelobaba I, Stojilković SS. Pituitary gonadotroph-specific patterns of gene expression and hormone secretion.. in Current Opinion in Pharmacology. 2022;66:102274.
doi:10.1016/j.coph.2022.102274 .

Constantin, Stephanie, Bjelobaba, Ivana, Stojilković, Stanko S., "Pituitary gonadotroph-specific patterns of gene expression and hormone secretion." in Current Opinion in Pharmacology, 66 (2022):102274,
https://doi.org/10.1016/j.coph.2022.102274 . .

TY  - JOUR
AU  - Milošević, Ana
AU  - Bjelobaba, Ivana
AU  - Božić, Iva
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Milošević, Katarina
AU  - Jakovljević, Marija
AU  - Stojilković, Stanko S.
AU  - Janjić, Marija
PY  - 2021
UR  - https://doi.org/10.1038/s41598-021-88305-5
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4248
AB  - Multiple sclerosis (MS) is an autoimmune disease that usually occurs during the reproductive years in both sexes. Many male patients with MS show lower blood testosterone levels, which was also observed in male rats during experimental autoimmune encephalomyelitis (EAE), an animal model of MS. To better understand the causes of decreased testosterone production during EAE, we investigated the expression status of genes and proteins associated with steroidogenesis in the testes. No changes in the number of interstitial cells were observed in EAE animals, but the expression of the insulin-like 3 gene was reduced at the peak of the disease, implying that the Leydig cell functional capacity was affected. Consistent with this finding, the expression of most steroidogenic enzyme genes and proteins was reduced during EAE, including StAR, CYP11A1, CYP17A1 and HSD3B. No signs of testicular inflammation were observed. Recovery of steroidogenesis was observed after injection of hCG, the placental gonadotropin, or buserelin acetate, a gonadotropin-releasing hormone analogue, at the peak of EAE. Together, our results are consistent with the hypothesis that impaired testicular steroidogenesis originates upstream of the testes and that low serum LH is the main cause of decreased testosterone levels during EAE.
PB  - Springer Science and Business Media LLC
T2  - Scientific Reports
T1  - Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-88305-5
SP  - 8996
ER  - 

@article{
author = "Milošević, Ana and Bjelobaba, Ivana and Božić, Iva and Lavrnja, Irena and Savić, Danijela and Milošević, Katarina and Jakovljević, Marija and Stojilković, Stanko S. and Janjić, Marija",
year = "2021",
abstract = "Multiple sclerosis (MS) is an autoimmune disease that usually occurs during the reproductive years in both sexes. Many male patients with MS show lower blood testosterone levels, which was also observed in male rats during experimental autoimmune encephalomyelitis (EAE), an animal model of MS. To better understand the causes of decreased testosterone production during EAE, we investigated the expression status of genes and proteins associated with steroidogenesis in the testes. No changes in the number of interstitial cells were observed in EAE animals, but the expression of the insulin-like 3 gene was reduced at the peak of the disease, implying that the Leydig cell functional capacity was affected. Consistent with this finding, the expression of most steroidogenic enzyme genes and proteins was reduced during EAE, including StAR, CYP11A1, CYP17A1 and HSD3B. No signs of testicular inflammation were observed. Recovery of steroidogenesis was observed after injection of hCG, the placental gonadotropin, or buserelin acetate, a gonadotropin-releasing hormone analogue, at the peak of EAE. Together, our results are consistent with the hypothesis that impaired testicular steroidogenesis originates upstream of the testes and that low serum LH is the main cause of decreased testosterone levels during EAE.",
publisher = "Springer Science and Business Media LLC",
journal = "Scientific Reports",
title = "Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-88305-5",
pages = "8996"
}

Milošević, A., Bjelobaba, I., Božić, I., Lavrnja, I., Savić, D., Milošević, K., Jakovljević, M., Stojilković, S. S.,& Janjić, M.. (2021). Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats. in Scientific Reports
Springer Science and Business Media LLC., 11(1), 8996.
https://doi.org/10.1038/s41598-021-88305-5

Milošević A, Bjelobaba I, Božić I, Lavrnja I, Savić D, Milošević K, Jakovljević M, Stojilković SS, Janjić M. Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats. in Scientific Reports. 2021;11(1):8996.
doi:10.1038/s41598-021-88305-5 .

Milošević, Ana, Bjelobaba, Ivana, Božić, Iva, Lavrnja, Irena, Savić, Danijela, Milošević, Katarina, Jakovljević, Marija, Stojilković, Stanko S., Janjić, Marija, "Testicular steroidogenesis is suppressed during experimental autoimmune encephalomyelitis in rats" in Scientific Reports, 11, no. 1 (2021):8996,
https://doi.org/10.1038/s41598-021-88305-5 . .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Janjić, Marija
AU  - Prévide, Rafael Maso
AU  - Abebe, Daniel
AU  - Kucka, Marek
AU  - Stojilković, Stanko S.
PY  - 2019
UR  - https://www.frontiersin.org/articles/10.3389/fendo.2019.00248/full
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3363
AB  - Cell-matrix interactions play important roles in pituitary development, physiology, and pathogenesis. In other tissues, a family of non-collagenous proteins, termed SIBLINGs, are known to contribute to cell-matrix interactions. Anterior pituitary gland expresses two SIBLING genes, Dmp1 (dentin matrix protein-1) and Spp1 (secreted phosphoprotein-1) encoding DMP1 and osteopontin proteins, respectively, but their expression pattern and roles in pituitary functions have not been clarified. Here we provide novel evidence supporting the conclusion that Spp1/osteopontin, like Dmp1/DMP1, are expressed in gonadotrophs in a sex- and age-specific manner. Other anterior pituitary cell types do not express these genes. In contrast to Dmp1, Spp1 expression is higher in males; in females, the expression reaches the peak during the diestrus phase of estrous cycle. In further contrast to Dmp1 and marker genes for gonadotrophs, the expression of Spp1 is not regulated by gonadotropin-releasing hormone in vivo and in vitro. However, Spp1 expression increases progressively after pituitary cell dispersion in both female and male cultures. We may speculate that gonadotrophs signal to other pituitary cell types about changes in the structure of pituitary cell-matrix network by osteopontin, a function consistent with the role of this secretory protein in postnatal tissue remodeling, extracellular matrix reorganization after injury, and tumorigenesis.
PB  - Frontiers Media S.A.
T2  - Frontiers in Endocrinology
T1  - Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs
IS  - MAR
VL  - 10
VL  - 10.3389/fendo.2019.00248
DO  - 10.3389/fendo.2019.00248
SP  - 248
ER  - 

@article{
author = "Bjelobaba, Ivana and Janjić, Marija and Prévide, Rafael Maso and Abebe, Daniel and Kucka, Marek and Stojilković, Stanko S.",
year = "2019",
abstract = "Cell-matrix interactions play important roles in pituitary development, physiology, and pathogenesis. In other tissues, a family of non-collagenous proteins, termed SIBLINGs, are known to contribute to cell-matrix interactions. Anterior pituitary gland expresses two SIBLING genes, Dmp1 (dentin matrix protein-1) and Spp1 (secreted phosphoprotein-1) encoding DMP1 and osteopontin proteins, respectively, but their expression pattern and roles in pituitary functions have not been clarified. Here we provide novel evidence supporting the conclusion that Spp1/osteopontin, like Dmp1/DMP1, are expressed in gonadotrophs in a sex- and age-specific manner. Other anterior pituitary cell types do not express these genes. In contrast to Dmp1, Spp1 expression is higher in males; in females, the expression reaches the peak during the diestrus phase of estrous cycle. In further contrast to Dmp1 and marker genes for gonadotrophs, the expression of Spp1 is not regulated by gonadotropin-releasing hormone in vivo and in vitro. However, Spp1 expression increases progressively after pituitary cell dispersion in both female and male cultures. We may speculate that gonadotrophs signal to other pituitary cell types about changes in the structure of pituitary cell-matrix network by osteopontin, a function consistent with the role of this secretory protein in postnatal tissue remodeling, extracellular matrix reorganization after injury, and tumorigenesis.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Endocrinology",
title = "Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs",
number = "MAR",
volume = "10, 10.3389/fendo.2019.00248",
doi = "10.3389/fendo.2019.00248",
pages = "248"
}

Bjelobaba, I., Janjić, M., Prévide, R. M., Abebe, D., Kucka, M.,& Stojilković, S. S.. (2019). Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs. in Frontiers in Endocrinology
Frontiers Media S.A.., 10(MAR), 248.
https://doi.org/10.3389/fendo.2019.00248

Bjelobaba I, Janjić M, Prévide RM, Abebe D, Kucka M, Stojilković SS. Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs. in Frontiers in Endocrinology. 2019;10(MAR):248.
doi:10.3389/fendo.2019.00248 .

Bjelobaba, Ivana, Janjić, Marija, Prévide, Rafael Maso, Abebe, Daniel, Kucka, Marek, Stojilković, Stanko S., "Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs" in Frontiers in Endocrinology, 10, no. MAR (2019):248,
https://doi.org/10.3389/fendo.2019.00248 . .

TY  - JOUR
AU  - Janjić, Marija
AU  - Prévide, Rafael M.
AU  - Fletcher, Patrick A.
AU  - Sherman, Arthur
AU  - Smiljanić, Kosara
AU  - Abebe, Daniel
AU  - Bjelobaba, Ivana
AU  - Stojilković, Stanko S.
PY  - 2019
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6934515
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3595
AB  - Continuous, as opposed to pulsatile, delivery of hypothalamic gonadotropin-releasing hormone (GnRH) leads to a marked decrease in secretion of pituitary gonadotropins LH and FSH and impairment of reproductive function. Here we studied the expression profile of gonadotropin subunit and GnRH receptor genes in rat pituitary in vitro and in vivo to clarify their expression profiles in the absence and continuous presence of GnRH. Culturing of pituitary cells in GnRH-free conditions downregulated Fshb, Cga, and Gnrhr expression, whereas continuous treatment with GnRH agonists upregulated Cga expression progressively and Gnrhr and Fshb expression transiently, accompanied by a prolonged blockade of Fshb but not Gnrhr expression. In contrast, Lhb expression was relatively insensitive to loss of endogenous GnRH and continuous treatment with GnRH, probably reflecting the status of Egr1 and Nr5a1 expression. Similar patterns of responses were observed in vivo after administration of a GnRH agonist. However, continuous treatment with GnRH stimulated LH secretion in vitro and in vivo, leading to decrease in LH cell content despite high basal Lhb expression. These data suggest that blockade of Fshb expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.
T2  - Scientific Reports
T1  - Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.
IS  - 1
VL  - 9
DO  - 10.1038/s41598-019-56480-1
SP  - 20098
ER  - 

@article{
author = "Janjić, Marija and Prévide, Rafael M. and Fletcher, Patrick A. and Sherman, Arthur and Smiljanić, Kosara and Abebe, Daniel and Bjelobaba, Ivana and Stojilković, Stanko S.",
year = "2019",
abstract = "Continuous, as opposed to pulsatile, delivery of hypothalamic gonadotropin-releasing hormone (GnRH) leads to a marked decrease in secretion of pituitary gonadotropins LH and FSH and impairment of reproductive function. Here we studied the expression profile of gonadotropin subunit and GnRH receptor genes in rat pituitary in vitro and in vivo to clarify their expression profiles in the absence and continuous presence of GnRH. Culturing of pituitary cells in GnRH-free conditions downregulated Fshb, Cga, and Gnrhr expression, whereas continuous treatment with GnRH agonists upregulated Cga expression progressively and Gnrhr and Fshb expression transiently, accompanied by a prolonged blockade of Fshb but not Gnrhr expression. In contrast, Lhb expression was relatively insensitive to loss of endogenous GnRH and continuous treatment with GnRH, probably reflecting the status of Egr1 and Nr5a1 expression. Similar patterns of responses were observed in vivo after administration of a GnRH agonist. However, continuous treatment with GnRH stimulated LH secretion in vitro and in vivo, leading to decrease in LH cell content despite high basal Lhb expression. These data suggest that blockade of Fshb expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.",
journal = "Scientific Reports",
title = "Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.",
number = "1",
volume = "9",
doi = "10.1038/s41598-019-56480-1",
pages = "20098"
}

Janjić, M., Prévide, R. M., Fletcher, P. A., Sherman, A., Smiljanić, K., Abebe, D., Bjelobaba, I.,& Stojilković, S. S.. (2019). Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.. in Scientific Reports, 9(1), 20098.
https://doi.org/10.1038/s41598-019-56480-1

Janjić M, Prévide RM, Fletcher PA, Sherman A, Smiljanić K, Abebe D, Bjelobaba I, Stojilković SS. Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.. in Scientific Reports. 2019;9(1):20098.
doi:10.1038/s41598-019-56480-1 .

Janjić, Marija, Prévide, Rafael M., Fletcher, Patrick A., Sherman, Arthur, Smiljanić, Kosara, Abebe, Daniel, Bjelobaba, Ivana, Stojilković, Stanko S., "Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo." in Scientific Reports, 9, no. 1 (2019):20098,
https://doi.org/10.1038/s41598-019-56480-1 . .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Li, Shuo
AU  - Stojilković, Stanko S.
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0005273617301098
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2745
AB  - Pannexins are a three-member family of vertebrate plasma membrane spanning molecules that have homology to the invertebrate gap junction forming proteins, the innexins. However, pannexins do not form gap junctions but operate as plasma membrane channels. The best-characterized member of these proteins, Pannexin1 (Panx1) was suggested to be functionally associated with purinergic P2X and N-methyl-D-aspartate (NMDA) receptor channels. Activation of these receptor channels by their endogenous ligands leads to cross-activation of Panx1 channels. This in turn potentiates P2X and NMDA receptor channel signaling. Two potentiation concepts have been suggested: enhancement of the current responses and/or sustained receptor channel activation by ATP released through Panx1 pore and adenosine generated by ectonucleotidase-dependent dephosphorylation of ATP. Here we summarize the current knowledge and hypotheses about interactions of Panx1 channels with P2X and NMDA receptor channels. This article is part of a Special Issue entitled: Gap Junction Proteins edited by Jean Claude Herve.
T2  - Biochimica et Biophysica Acta (BBA) - Biomembranes
T1  - Interactions of Pannexin1 channels with purinergic and NMDA receptor channels
IS  - 1
VL  - 1860
DO  - 10.1016/j.bbamem.2017.03.025
SP  - 166
EP  - 173
ER  - 

@article{
author = "Bjelobaba, Ivana and Li, Shuo and Stojilković, Stanko S.",
year = "2018",
abstract = "Pannexins are a three-member family of vertebrate plasma membrane spanning molecules that have homology to the invertebrate gap junction forming proteins, the innexins. However, pannexins do not form gap junctions but operate as plasma membrane channels. The best-characterized member of these proteins, Pannexin1 (Panx1) was suggested to be functionally associated with purinergic P2X and N-methyl-D-aspartate (NMDA) receptor channels. Activation of these receptor channels by their endogenous ligands leads to cross-activation of Panx1 channels. This in turn potentiates P2X and NMDA receptor channel signaling. Two potentiation concepts have been suggested: enhancement of the current responses and/or sustained receptor channel activation by ATP released through Panx1 pore and adenosine generated by ectonucleotidase-dependent dephosphorylation of ATP. Here we summarize the current knowledge and hypotheses about interactions of Panx1 channels with P2X and NMDA receptor channels. This article is part of a Special Issue entitled: Gap Junction Proteins edited by Jean Claude Herve.",
journal = "Biochimica et Biophysica Acta (BBA) - Biomembranes",
title = "Interactions of Pannexin1 channels with purinergic and NMDA receptor channels",
number = "1",
volume = "1860",
doi = "10.1016/j.bbamem.2017.03.025",
pages = "166-173"
}

Bjelobaba, I., Li, S.,& Stojilković, S. S.. (2018). Interactions of Pannexin1 channels with purinergic and NMDA receptor channels. in Biochimica et Biophysica Acta (BBA) - Biomembranes, 1860(1), 166-173.
https://doi.org/10.1016/j.bbamem.2017.03.025

Bjelobaba I, Li S, Stojilković SS. Interactions of Pannexin1 channels with purinergic and NMDA receptor channels. in Biochimica et Biophysica Acta (BBA) - Biomembranes. 2018;1860(1):166-173.
doi:10.1016/j.bbamem.2017.03.025 .

Bjelobaba, Ivana, Li, Shuo, Stojilković, Stanko S., "Interactions of Pannexin1 channels with purinergic and NMDA receptor channels" in Biochimica et Biophysica Acta (BBA) - Biomembranes, 1860, no. 1 (2018):166-173,
https://doi.org/10.1016/j.bbamem.2017.03.025 . .

TY  - GEN
AU  - Bjelobaba, Ivana
AU  - Stojilković, Stanko S.
AU  - Naor, Zvi
PY  - 2018
UR  - http://journal.frontiersin.org/article/10.3389/fendo.2018.00143/full
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3039
AB  - [No abstract available]
T2  - Frontiers in Endocrinology
T1  - Editorial: Gonadotropin-Releasing Hormone Receptor Signaling and Functions
IS  - April
VL  - 9
DO  - 10.3389/fendo.2018.00143
SP  - 143
ER  - 

@misc{
author = "Bjelobaba, Ivana and Stojilković, Stanko S. and Naor, Zvi",
year = "2018",
abstract = "[No abstract available]",
journal = "Frontiers in Endocrinology",
title = "Editorial: Gonadotropin-Releasing Hormone Receptor Signaling and Functions",
number = "April",
volume = "9",
doi = "10.3389/fendo.2018.00143",
pages = "143"
}

Bjelobaba, I., Stojilković, S. S.,& Naor, Z.. (2018). Editorial: Gonadotropin-Releasing Hormone Receptor Signaling and Functions. in Frontiers in Endocrinology, 9(April), 143.
https://doi.org/10.3389/fendo.2018.00143

Bjelobaba I, Stojilković SS, Naor Z. Editorial: Gonadotropin-Releasing Hormone Receptor Signaling and Functions. in Frontiers in Endocrinology. 2018;9(April):143.
doi:10.3389/fendo.2018.00143 .

Bjelobaba, Ivana, Stojilković, Stanko S., Naor, Zvi, "Editorial: Gonadotropin-Releasing Hormone Receptor Signaling and Functions" in Frontiers in Endocrinology, 9, no. April (2018):143,
https://doi.org/10.3389/fendo.2018.00143 . .

TY  - JOUR
AU  - Janjić, Marija
AU  - Stojilković, Stanko S.
AU  - Bjelobaba, Ivana
PY  - 2017
UR  - http://journal.frontiersin.org/article/10.3389/fendo.2017.00221/full
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2853
AB  - The hypothalamic decapeptide gonadotropin-releasing hormone (GnRH), acting via its receptors (GnRHRs) expressed in pituitary gonadotrophs, represents a critical molecule in control of reproductive functions in all vertebrate species. GnRH-activated receptors regulate synthesis of gonadotropins in a frequency-dependent manner. The number of GnRHRs on the plasma membrane determines the responsiveness of gonadotrophs to GnRH and varies in relation to age, sex, and physiological status. This is achieved by a complex control that operates at transcriptional, translational, and posttranslational levels. This review aims to overview the mechanisms of GnRHR gene (Gnrhr) transcription in mammalian gonadotrophs. In general, Gnrhr exhibits basal and regulated transcription activities. Basal Gnrhr transcription appears to be an intrinsic property of native and immortalized gonadotrophs that secures the presence of a sufficient number GnRHRs to preserve their functionality independently of the status of regulated transcription. On the other hand, regulated transcription modulates GnRHR expression during development, reproductive cycle, and aging. GnRH is crucial for regulated Gnrhr transcription in native gonadotrophs but is ineffective in immortalized gonadotrophs. In rat and mouse, both basal and GnRH-induced Gnrhr transcription rely primarily on the protein kinase C signaling pathway, with subsequent activation of mitogen-activated protein kinases. Continuous GnRH application, after a transient stimulation, shuts offregulated but not basal transcription, suggesting that different branches of this signaling pathway control transcription. Pituitary adenylate cyclase-activating polypeptide, but not activins, contributes to the regulated transcription utilizing the protein kinase A signaling pathway, whereas a mechanisms by which steroid hormones modulate Gnrhr transcription has not been well characterized.
T2  - Frontiers in Endocrinology
T1  - Intrinsic and Regulated Gonadotropin-Releasing Hormone Receptor Gene Transcription in Mammalian Pituitary Gonadotrophs
IS  - SEP
VL  - 8
DO  - 10.3389/fendo.2017.00221
SP  - 221
EP  - 221
ER  - 

@article{
author = "Janjić, Marija and Stojilković, Stanko S. and Bjelobaba, Ivana",
year = "2017",
abstract = "The hypothalamic decapeptide gonadotropin-releasing hormone (GnRH), acting via its receptors (GnRHRs) expressed in pituitary gonadotrophs, represents a critical molecule in control of reproductive functions in all vertebrate species. GnRH-activated receptors regulate synthesis of gonadotropins in a frequency-dependent manner. The number of GnRHRs on the plasma membrane determines the responsiveness of gonadotrophs to GnRH and varies in relation to age, sex, and physiological status. This is achieved by a complex control that operates at transcriptional, translational, and posttranslational levels. This review aims to overview the mechanisms of GnRHR gene (Gnrhr) transcription in mammalian gonadotrophs. In general, Gnrhr exhibits basal and regulated transcription activities. Basal Gnrhr transcription appears to be an intrinsic property of native and immortalized gonadotrophs that secures the presence of a sufficient number GnRHRs to preserve their functionality independently of the status of regulated transcription. On the other hand, regulated transcription modulates GnRHR expression during development, reproductive cycle, and aging. GnRH is crucial for regulated Gnrhr transcription in native gonadotrophs but is ineffective in immortalized gonadotrophs. In rat and mouse, both basal and GnRH-induced Gnrhr transcription rely primarily on the protein kinase C signaling pathway, with subsequent activation of mitogen-activated protein kinases. Continuous GnRH application, after a transient stimulation, shuts offregulated but not basal transcription, suggesting that different branches of this signaling pathway control transcription. Pituitary adenylate cyclase-activating polypeptide, but not activins, contributes to the regulated transcription utilizing the protein kinase A signaling pathway, whereas a mechanisms by which steroid hormones modulate Gnrhr transcription has not been well characterized.",
journal = "Frontiers in Endocrinology",
title = "Intrinsic and Regulated Gonadotropin-Releasing Hormone Receptor Gene Transcription in Mammalian Pituitary Gonadotrophs",
number = "SEP",
volume = "8",
doi = "10.3389/fendo.2017.00221",
pages = "221-221"
}

Janjić, M., Stojilković, S. S.,& Bjelobaba, I.. (2017). Intrinsic and Regulated Gonadotropin-Releasing Hormone Receptor Gene Transcription in Mammalian Pituitary Gonadotrophs. in Frontiers in Endocrinology, 8(SEP), 221-221.
https://doi.org/10.3389/fendo.2017.00221

Janjić M, Stojilković SS, Bjelobaba I. Intrinsic and Regulated Gonadotropin-Releasing Hormone Receptor Gene Transcription in Mammalian Pituitary Gonadotrophs. in Frontiers in Endocrinology. 2017;8(SEP):221-221.
doi:10.3389/fendo.2017.00221 .

Janjić, Marija, Stojilković, Stanko S., Bjelobaba, Ivana, "Intrinsic and Regulated Gonadotropin-Releasing Hormone Receptor Gene Transcription in Mammalian Pituitary Gonadotrophs" in Frontiers in Endocrinology, 8, no. SEP (2017):221-221,
https://doi.org/10.3389/fendo.2017.00221 . .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Stojilković, Stanko S.
AU  - Zemkova, Hana
PY  - 2017
UR  - http://journal.frontiersin.org/article/10.3389/fendo.2017.00126/full
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2783
AB  - Gonadotrophs are basophilic cells of the anterior pituitary gland specialized to secrete gonadotropins in response to elevation in intracellular calcium concentration. These cells fire action potentials (APs) spontaneously, coupled with voltage-gated calcium influx of insufficient amplitude to trigger gonadotropin release. The spontaneous excitability of gonadotrophs reflects the expression of voltage-gated sodium, calcium, potassium, non-selective cation-conducting, and chloride channels at their plasma membrane (PM). These cells also express the hyperpolarization-activated and cyclic nucleotide-gated cation channels at the PM, as well as GABAA, nicotinic, and purinergic P2X channels gated by γ-aminobutyric acid (GABA), acetylcholine (ACh), and ATP, respectively. Activation of these channels leads to initiation or amplification of the pacemaking activity, facilitation of calcium influx, and activation of the exocytic pathway. Gonadotrophs also express calcium-conducting channels at the endoplasmic reticulum membranes gated by inositol trisphosphate and intracellular calcium. These channels are activated potently by hypothalamic gonadotropin-releasing hormone (GnRH) and less potently by several paracrine calcium-mobilizing agonists, including pituitary adenylate cyclase-activating peptides, endothelins, ACh, vasopressin, and oxytocin. Activation of these channels causes oscillatory calcium release and a rapid gonadotropin release, accompanied with a shift from tonic firing of single APs to periodic bursting type of electrical activity, which accounts for a sustained calcium signaling and gonadotropin secretion. This review summarizes our current understanding of ion channels as signaling molecules in gonadotrophs, the role of GnRH and paracrine agonists in their gating, and the cross talk among channels.
T2  - Frontiers in Endocrinology
T1  - Ion Channels of Pituitary Gonadotrophs and Their Roles in Signaling and Secretion
IS  - JUN
VL  - 8
DO  - 10.3389/fendo.2017.00126
SP  - 126
EP  - 126
ER  - 

@article{
author = "Bjelobaba, Ivana and Stojilković, Stanko S. and Zemkova, Hana",
year = "2017",
abstract = "Gonadotrophs are basophilic cells of the anterior pituitary gland specialized to secrete gonadotropins in response to elevation in intracellular calcium concentration. These cells fire action potentials (APs) spontaneously, coupled with voltage-gated calcium influx of insufficient amplitude to trigger gonadotropin release. The spontaneous excitability of gonadotrophs reflects the expression of voltage-gated sodium, calcium, potassium, non-selective cation-conducting, and chloride channels at their plasma membrane (PM). These cells also express the hyperpolarization-activated and cyclic nucleotide-gated cation channels at the PM, as well as GABAA, nicotinic, and purinergic P2X channels gated by γ-aminobutyric acid (GABA), acetylcholine (ACh), and ATP, respectively. Activation of these channels leads to initiation or amplification of the pacemaking activity, facilitation of calcium influx, and activation of the exocytic pathway. Gonadotrophs also express calcium-conducting channels at the endoplasmic reticulum membranes gated by inositol trisphosphate and intracellular calcium. These channels are activated potently by hypothalamic gonadotropin-releasing hormone (GnRH) and less potently by several paracrine calcium-mobilizing agonists, including pituitary adenylate cyclase-activating peptides, endothelins, ACh, vasopressin, and oxytocin. Activation of these channels causes oscillatory calcium release and a rapid gonadotropin release, accompanied with a shift from tonic firing of single APs to periodic bursting type of electrical activity, which accounts for a sustained calcium signaling and gonadotropin secretion. This review summarizes our current understanding of ion channels as signaling molecules in gonadotrophs, the role of GnRH and paracrine agonists in their gating, and the cross talk among channels.",
journal = "Frontiers in Endocrinology",
title = "Ion Channels of Pituitary Gonadotrophs and Their Roles in Signaling and Secretion",
number = "JUN",
volume = "8",
doi = "10.3389/fendo.2017.00126",
pages = "126-126"
}

Bjelobaba, I., Stojilković, S. S.,& Zemkova, H.. (2017). Ion Channels of Pituitary Gonadotrophs and Their Roles in Signaling and Secretion. in Frontiers in Endocrinology, 8(JUN), 126-126.
https://doi.org/10.3389/fendo.2017.00126

Bjelobaba I, Stojilković SS, Zemkova H. Ion Channels of Pituitary Gonadotrophs and Their Roles in Signaling and Secretion. in Frontiers in Endocrinology. 2017;8(JUN):126-126.
doi:10.3389/fendo.2017.00126 .

Bjelobaba, Ivana, Stojilković, Stanko S., Zemkova, Hana, "Ion Channels of Pituitary Gonadotrophs and Their Roles in Signaling and Secretion" in Frontiers in Endocrinology, 8, no. JUN (2017):126-126,
https://doi.org/10.3389/fendo.2017.00126 . .