TY  - JOUR
AU  - Katanić, Jelena
AU  - Matić, Sanja
AU  - Pferschy-Wenzig, Eva-Maria
AU  - Kretschmer, Nadine
AU  - Boroja, Tatjana
AU  - Mihailović, Vladimir
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Bauer, Rudolf
PY  - 2017
UR  - https://www.sciencedirect.com/science/article/pii/S0278691516304343?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3178
AB  - Filipendula ulmaria, known as meadowsweet, is a perennial herb found in wild and cultivated habitats in Europe and Asia. Usage of F. ulmaria in traditional medicine is based on diuretic, astringent, antirheumatic, and anti-inflammatory properties of this plant. Exposure to cisplatin at a dose of 7.5 mg/kg caused significant increase in serum parameters of liver and kidneys function and tissue oxidative stress markers along with some histopathological changes in liver and kidney tissues of experimental rats, as well as high level of genotoxicity. Administration of F. ulmaria extracts in three different concentrations (100, 200, and 400 mg/kg/day) for 10 days resulted in a reduction of oxidative stress in tissues and decrease of serum parameters. Moreover, tested extracts attenuated the genotoxicity of cisplatin in reverse dose-dependent manner. F. ulmaria extracts had no in vitro cytotoxic activity at all applied concentrations (IC50 > 50 μg/mL). Tested extracts, rich in polyphenolic compounds, attenuate cisplatin-induced liver and kidney oxidative stress, reduce tissue damage, and enhance the antioxidative status of experimental animals during cisplatin application. Therefore, F. ulmaria extracts may be used as supportive agent for the prevention and amelioration of cisplatin side effects.
T2  - Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association
T1  - Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.
VL  - 99
DO  - 10.1016/j.fct.2016.11.018
SP  - 86
EP  - 102
ER  - 

@article{
author = "Katanić, Jelena and Matić, Sanja and Pferschy-Wenzig, Eva-Maria and Kretschmer, Nadine and Boroja, Tatjana and Mihailović, Vladimir and Stanković, Vesna and Stanković, Nevena and Mladenović, Milan and Stanić, Snežana and Mihailović, Mirjana and Bauer, Rudolf",
year = "2017",
abstract = "Filipendula ulmaria, known as meadowsweet, is a perennial herb found in wild and cultivated habitats in Europe and Asia. Usage of F. ulmaria in traditional medicine is based on diuretic, astringent, antirheumatic, and anti-inflammatory properties of this plant. Exposure to cisplatin at a dose of 7.5 mg/kg caused significant increase in serum parameters of liver and kidneys function and tissue oxidative stress markers along with some histopathological changes in liver and kidney tissues of experimental rats, as well as high level of genotoxicity. Administration of F. ulmaria extracts in three different concentrations (100, 200, and 400 mg/kg/day) for 10 days resulted in a reduction of oxidative stress in tissues and decrease of serum parameters. Moreover, tested extracts attenuated the genotoxicity of cisplatin in reverse dose-dependent manner. F. ulmaria extracts had no in vitro cytotoxic activity at all applied concentrations (IC50 > 50 μg/mL). Tested extracts, rich in polyphenolic compounds, attenuate cisplatin-induced liver and kidney oxidative stress, reduce tissue damage, and enhance the antioxidative status of experimental animals during cisplatin application. Therefore, F. ulmaria extracts may be used as supportive agent for the prevention and amelioration of cisplatin side effects.",
journal = "Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association",
title = "Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.",
volume = "99",
doi = "10.1016/j.fct.2016.11.018",
pages = "86-102"
}

Katanić, J., Matić, S., Pferschy-Wenzig, E., Kretschmer, N., Boroja, T., Mihailović, V., Stanković, V., Stanković, N., Mladenović, M., Stanić, S., Mihailović, M.,& Bauer, R.. (2017). Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.. in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association, 99, 86-102.
https://doi.org/10.1016/j.fct.2016.11.018

Katanić J, Matić S, Pferschy-Wenzig E, Kretschmer N, Boroja T, Mihailović V, Stanković V, Stanković N, Mladenović M, Stanić S, Mihailović M, Bauer R. Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis.. in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association. 2017;99:86-102.
doi:10.1016/j.fct.2016.11.018 .

Katanić, Jelena, Matić, Sanja, Pferschy-Wenzig, Eva-Maria, Kretschmer, Nadine, Boroja, Tatjana, Mihailović, Vladimir, Stanković, Vesna, Stanković, Nevena, Mladenović, Milan, Stanić, Snežana, Mihailović, Mirjana, Bauer, Rudolf, "Filipendula ulmaria extracts attenuate cisplatin-induced liver and kidney oxidative stress in rats: In vivo investigation and LC-MS analysis." in Food and Chemical Toxicology : an international journal published for the British Industrial Biological Research Association, 99 (2017):86-102,
https://doi.org/10.1016/j.fct.2016.11.018 . .

TY  - GEN
AU  - Katanić, Jelena
AU  - Mihailović, Vladimir
AU  - Matić, Sanja
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Mladenović, Milan
AU  - Stanić, Snežana
AU  - Kreft, Samo
AU  - Mihailović, Mirjana
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S1756464616303644
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2841
AB  - The authors regret that in Fig. 3 inadvertently was incorporated two photographs of the same tissue preparation of group VII (Fig. 3VII and X). They would like to inform that this wrong figure did not change the results of their study. The accurate representative photograph of kidney sections of experimental animals from group X (Fig. 3X in Fig. 3) is given below. The authors would like to apologize for any inconvenience caused.
T2  - Journal of Functional Foods
T1  - Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]
VL  - 28
DO  - 10.1016/j.jff.2016.11.017
SP  - 326
EP  - 327
ER  - 

@misc{
author = "Katanić, Jelena and Mihailović, Vladimir and Matić, Sanja and Stanković, Vesna and Stanković, Nevena and Boroja, Tatjana and Mladenović, Milan and Stanić, Snežana and Kreft, Samo and Mihailović, Mirjana",
year = "2017",
abstract = "The authors regret that in Fig. 3 inadvertently was incorporated two photographs of the same tissue preparation of group VII (Fig. 3VII and X). They would like to inform that this wrong figure did not change the results of their study. The accurate representative photograph of kidney sections of experimental animals from group X (Fig. 3X in Fig. 3) is given below. The authors would like to apologize for any inconvenience caused.",
journal = "Journal of Functional Foods",
title = "Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]",
volume = "28",
doi = "10.1016/j.jff.2016.11.017",
pages = "326-327"
}

Katanić, J., Mihailović, V., Matić, S., Stanković, V., Stanković, N., Boroja, T., Mladenović, M., Stanić, S., Kreft, S.,& Mihailović, M.. (2017). Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]. in Journal of Functional Foods, 28, 326-327.
https://doi.org/10.1016/j.jff.2016.11.017

Katanić J, Mihailović V, Matić S, Stanković V, Stanković N, Boroja T, Mladenović M, Stanić S, Kreft S, Mihailović M. Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]. in Journal of Functional Foods. 2017;28:326-327.
doi:10.1016/j.jff.2016.11.017 .

Katanić, Jelena, Mihailović, Vladimir, Matić, Sanja, Stanković, Vesna, Stanković, Nevena, Boroja, Tatjana, Mladenović, Milan, Stanić, Snežana, Kreft, Samo, Mihailović, Mirjana, "Corrigendum to “The ameliorating effect of Filipendula hexapetala extracts on hepatorenal toxicity of cisplatin” [J. Funct. Foods 18(A) (2015) 198–212]" in Journal of Functional Foods, 28 (2017):326-327,
https://doi.org/10.1016/j.jff.2016.11.017 . .

TY  - JOUR
AU  - Milošev, Milorad Z
AU  - Jakovljević, Katarina
AU  - Joksović, Milan D
AU  - Stanojković, Tatjana
AU  - Matić, Ivana Z
AU  - Perović, Milka
AU  - Tešić, Vesna
AU  - Kanazir, Selma
AU  - Mladenović, Milan
AU  - Rodić, Marko V
AU  - Leovac, Vukadin M
AU  - Trifunović, Snežana
AU  - Marković, Violeta
PY  - 2017
UR  - http://doi.wiley.com/10.1111/cbdd.12920
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27933733
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2753
AB  - A series of 18 novel N-Mannich bases derived from 5-adamantyl-1,2,4-triazole-3-thione was synthesized and characterized using NMR spectroscopy and X-ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL-60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC-5 compared to cancer cells. The effects of compounds 5b, 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase-dependent apoptosis, while the anti-angiogenic effects of 5b, 5e, and 5j have been confirmed in EA.hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.
T2  - Chemical Biology & Drug Design
T1  - Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies
IS  - 6
VL  - 89
DO  - 10.1111/cbdd.12920
SP  - 943
EP  - 952
ER  - 

@article{
author = "Milošev, Milorad Z and Jakovljević, Katarina and Joksović, Milan D and Stanojković, Tatjana and Matić, Ivana Z and Perović, Milka and Tešić, Vesna and Kanazir, Selma and Mladenović, Milan and Rodić, Marko V and Leovac, Vukadin M and Trifunović, Snežana and Marković, Violeta",
year = "2017",
abstract = "A series of 18 novel N-Mannich bases derived from 5-adamantyl-1,2,4-triazole-3-thione was synthesized and characterized using NMR spectroscopy and X-ray diffraction technique. All derivatives were evaluated for their anticancer potential against four human cancer cell lines. Several tested compounds exerted good cytotoxic activities on K562 and HL-60 cell lines, along with pronounced selectivity, showing lower cytotoxicity against normal fibroblasts MRC-5 compared to cancer cells. The effects of compounds 5b, 5e, and 5j on the cell cycle were investigated by flow cytometric analysis. It was found that these compounds cause the accumulation of cells in the subG1 and G1 phases of the cell cycle and induce caspase-dependent apoptosis, while the anti-angiogenic effects of 5b, 5e, and 5j have been confirmed in EA.hy926 cells using a tube formation assay. Further, the interaction of Bax protein with compound 5b was investigated by means of molecular modeling, applying the combined molecular docking/molecular dynamics approach.",
journal = "Chemical Biology & Drug Design",
title = "Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies",
number = "6",
volume = "89",
doi = "10.1111/cbdd.12920",
pages = "943-952"
}

Milošev, M. Z., Jakovljević, K., Joksović, M. D., Stanojković, T., Matić, I. Z., Perović, M., Tešić, V., Kanazir, S., Mladenović, M., Rodić, M. V., Leovac, V. M., Trifunović, S.,& Marković, V.. (2017). Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies. in Chemical Biology & Drug Design, 89(6), 943-952.
https://doi.org/10.1111/cbdd.12920

Milošev MZ, Jakovljević K, Joksović MD, Stanojković T, Matić IZ, Perović M, Tešić V, Kanazir S, Mladenović M, Rodić MV, Leovac VM, Trifunović S, Marković V. Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies. in Chemical Biology & Drug Design. 2017;89(6):943-952.
doi:10.1111/cbdd.12920 .

Milošev, Milorad Z, Jakovljević, Katarina, Joksović, Milan D, Stanojković, Tatjana, Matić, Ivana Z, Perović, Milka, Tešić, Vesna, Kanazir, Selma, Mladenović, Milan, Rodić, Marko V, Leovac, Vukadin M, Trifunović, Snežana, Marković, Violeta, "Mannich bases of 1,2,4-triazole-3-thione containing adamantane moiety: Synthesis, preliminary anticancer evaluation, and molecular modeling studies" in Chemical Biology & Drug Design, 89, no. 6 (2017):943-952,
https://doi.org/10.1111/cbdd.12920 . .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Mihailović, Mirjana
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Boroja, Tatjana
AU  - Vuković, Nenad
PY  - 2015
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-84943457635&partnerID=tZOtx3y1
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0006295215005511
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3175
AB  - Eight chroman-2,4-diones, namely 2a-h, previously investigated as anticoagulants, of which 2a and 2f as the most active, were evaluated as in vivo genotoxic agents in Wistar rat livers and kidneys using the comet assay. Compounds 2a, 2b, and 2f without genotoxic activity were applied prior to ethyl methanesulfonate (EMS) and diminished EMS-induced DNA damage according to the total score and percentage of reduction. EMS produce harmful O(6)-ethylguanine lesion which is incorporated in aberrant genotoxic GT and TG pairing after ATP-dependent DNA strand breaks have been catalyzed by rat Topoisomerase IIα (rTopIIα, EC 5.99.1.3). Therefore, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on the enzyme level using molecular docking and molecular dynamics simulations insamuch as it had been determined that compounds do not intercalate DNA but instead inhibit the ATPase activity. Calculations predicted that compounds inhibit ATP hydrolysis before the DNA-EMS cleavage is being catalyzed by rTopIIα, prevent EMS mutagenic and carcinogenic effects, and beside anticoagulant activity can even be applied in the cancer treatment to control the rate of anticancer alkylation drugs.
PB  - Elsevier
T2  - Biochemical Pharmacology
T1  - Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach
IS  - 1
VL  - 98
DO  - 10.1016/j.bcp.2015.08.106
SP  - 243
EP  - 266
ER  - 

@article{
author = "Mladenović, Milan and Stanković, Nevena and Matić, Sanja and Stanić, Snežana and Mihailović, Mirjana and Mihailović, Vladimir and Katanić, Jelena and Boroja, Tatjana and Vuković, Nenad",
year = "2015",
abstract = "Eight chroman-2,4-diones, namely 2a-h, previously investigated as anticoagulants, of which 2a and 2f as the most active, were evaluated as in vivo genotoxic agents in Wistar rat livers and kidneys using the comet assay. Compounds 2a, 2b, and 2f without genotoxic activity were applied prior to ethyl methanesulfonate (EMS) and diminished EMS-induced DNA damage according to the total score and percentage of reduction. EMS produce harmful O(6)-ethylguanine lesion which is incorporated in aberrant genotoxic GT and TG pairing after ATP-dependent DNA strand breaks have been catalyzed by rat Topoisomerase IIα (rTopIIα, EC 5.99.1.3). Therefore, the mechanism of 2a, 2b, and 2f antigenotoxic activity was investigated on the enzyme level using molecular docking and molecular dynamics simulations insamuch as it had been determined that compounds do not intercalate DNA but instead inhibit the ATPase activity. Calculations predicted that compounds inhibit ATP hydrolysis before the DNA-EMS cleavage is being catalyzed by rTopIIα, prevent EMS mutagenic and carcinogenic effects, and beside anticoagulant activity can even be applied in the cancer treatment to control the rate of anticancer alkylation drugs.",
publisher = "Elsevier",
journal = "Biochemical Pharmacology",
title = "Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach",
number = "1",
volume = "98",
doi = "10.1016/j.bcp.2015.08.106",
pages = "243-266"
}

Mladenović, M., Stanković, N., Matić, S., Stanić, S., Mihailović, M., Mihailović, V., Katanić, J., Boroja, T.,& Vuković, N.. (2015). Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach. in Biochemical Pharmacology
Elsevier., 98(1), 243-266.
https://doi.org/10.1016/j.bcp.2015.08.106

Mladenović M, Stanković N, Matić S, Stanić S, Mihailović M, Mihailović V, Katanić J, Boroja T, Vuković N. Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach. in Biochemical Pharmacology. 2015;98(1):243-266.
doi:10.1016/j.bcp.2015.08.106 .

Mladenović, Milan, Stanković, Nevena, Matić, Sanja, Stanić, Snežana, Mihailović, Mirjana, Mihailović, Vladimir, Katanić, Jelena, Boroja, Tatjana, Vuković, Nenad, "Newly discovered chroman-2,4-diones neutralize the in vivo DNA damage induced by alkylation through the inhibition of Topoisomerase IIα: A story behind the molecular modeling approach" in Biochemical Pharmacology, 98, no. 1 (2015):243-266,
https://doi.org/10.1016/j.bcp.2015.08.106 . .

TY  - JOUR
AU  - Katanić, Jelena
AU  - Mihailović, Vladimir
AU  - Matić, Sanja
AU  - Stanković, Vesna
AU  - Stanković, Nevena
AU  - Boroja, Tatjana
AU  - Mladenović, Milan
AU  - Stanić, Snezana
AU  - Kreft, Samo
AU  - Mihailović, Mirjana
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2352
UR  - http://www.sciencedirect.com/science/article/pii/S175646461500362X
AB  - The effects of the methanolic extracts of Filipendula hexapetala Gilib.
   aerial parts (FHA) and roots (FHR) against cisplatin induced kidney and
   liver injuries in rats were investigated as well as determination of
   genotoxicity and antigenotoxicity of the extracts. Treatment with FHA
   and FHR significantly decreased levels of urea, uric acid, serum
   transaminases, alkaline phosphatase and gamma-glutamyl transferase, and
   increased the content of total protein. In addition, treatment with the
   extracts significantly attenuated the cisplatin-induced oxidative stress
   in kidney and liver tissues by increasing catalase and superoxide
   dismutase activities and the content of reduced glutathione and
   decreasing the content of thiobarbituric acid reactive substances
   (TSARS). The histopathological studies confirmed the protective effects
   of the extracts against cisplatin-induced kidney and liver injuries. The
   extracts ameliorated cisplatin-induced genotoxicity. These results
   suggest that F. hexapetala extracts are effective nephro- and
   hepatoprotective agents, with potential to reduce oxidative stress and
   ameliorate cisplatin-induced nephro- and hepatotoxicity.
T2  - Journal of Functional Foods
T1  - The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin
IS  - Part A
VL  - 18
DO  - 10.1016/j.jff.2015.07.004
SP  - 198
EP  - 212
ER  - 

@article{
author = "Katanić, Jelena and Mihailović, Vladimir and Matić, Sanja and Stanković, Vesna and Stanković, Nevena and Boroja, Tatjana and Mladenović, Milan and Stanić, Snezana and Kreft, Samo and Mihailović, Mirjana",
year = "2015",
abstract = "The effects of the methanolic extracts of Filipendula hexapetala Gilib.
   aerial parts (FHA) and roots (FHR) against cisplatin induced kidney and
   liver injuries in rats were investigated as well as determination of
   genotoxicity and antigenotoxicity of the extracts. Treatment with FHA
   and FHR significantly decreased levels of urea, uric acid, serum
   transaminases, alkaline phosphatase and gamma-glutamyl transferase, and
   increased the content of total protein. In addition, treatment with the
   extracts significantly attenuated the cisplatin-induced oxidative stress
   in kidney and liver tissues by increasing catalase and superoxide
   dismutase activities and the content of reduced glutathione and
   decreasing the content of thiobarbituric acid reactive substances
   (TSARS). The histopathological studies confirmed the protective effects
   of the extracts against cisplatin-induced kidney and liver injuries. The
   extracts ameliorated cisplatin-induced genotoxicity. These results
   suggest that F. hexapetala extracts are effective nephro- and
   hepatoprotective agents, with potential to reduce oxidative stress and
   ameliorate cisplatin-induced nephro- and hepatotoxicity.",
journal = "Journal of Functional Foods",
title = "The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin",
number = "Part A",
volume = "18",
doi = "10.1016/j.jff.2015.07.004",
pages = "198-212"
}

Katanić, J., Mihailović, V., Matić, S., Stanković, V., Stanković, N., Boroja, T., Mladenović, M., Stanić, S., Kreft, S.,& Mihailović, M.. (2015). The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin. in Journal of Functional Foods, 18(Part A), 198-212.
https://doi.org/10.1016/j.jff.2015.07.004

Katanić J, Mihailović V, Matić S, Stanković V, Stanković N, Boroja T, Mladenović M, Stanić S, Kreft S, Mihailović M. The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin. in Journal of Functional Foods. 2015;18(Part A):198-212.
doi:10.1016/j.jff.2015.07.004 .

Katanić, Jelena, Mihailović, Vladimir, Matić, Sanja, Stanković, Vesna, Stanković, Nevena, Boroja, Tatjana, Mladenović, Milan, Stanić, Snezana, Kreft, Samo, Mihailović, Mirjana, "The ameliorating effect of Filipendula hexapetala extracts on
 hepatorenal toxicity of cisplatin" in Journal of Functional Foods, 18, no. Part A (2015):198-212,
https://doi.org/10.1016/j.jff.2015.07.004 . .

TY  - JOUR
AU  - Stanković, Nevena
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Arambašić Jovanović, Jelena
AU  - Uskoković, Aleksandra
AU  - Stanković, Vesna
AU  - Mihailović, Vladimir
AU  - Katanić, Jelena
AU  - Matić, Sanja
AU  - Solujić, Slavica
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
PY  - 2014
UR  - http://www.ncbi.nlm.nih.gov/pubmed/24468630
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3185
AB  - Eight synthesized 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones were evaluated as in vivo anticoagulants by intraperitoneal application to adult male Wistar rats in order to examine their pharmacological potential, evaluate ther toxicity and propose the mechanism of action. Two of them, 2f and 2a, in concentration of 2mg/kg of body weight, presented remarkable activity (PT=130s; PT=90s) upon seven days of continuous application. The results of rat serum and liver biochemical screening, as well those of histopathological studies, proved the compounds to be non-toxic. Activity of the compounds was further examined on the molecular level. Here, molecular docking studies were performed to position the compounds in relation to the active site of VKORC1 and determine the bioactive conformations. Docking results suggested a non-covalent mode of action during which the proton transfer occurs from Cys135 SH towards 4-carbonyl group of anticoagulant. All crucial interactions for anticoagulant activity were confirmed in generated structure-based 3-D pharmacophore model, consisted of hydrogen bond acceptor and hydrophobic aromatic features, and quantified by a best correlation coefficient of 0.97.
PB  - Elsevier
T2  - European Journal of Pharmaceutical Sciences
T1  - Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.
IS  - 1
VL  - 55
DO  - 10.1016/j.ejps.2014.01.004
SP  - 20
EP  - 35
ER  - 

@article{
author = "Stanković, Nevena and Mladenović, Milan and Mihailović, Mirjana and Arambašić Jovanović, Jelena and Uskoković, Aleksandra and Stanković, Vesna and Mihailović, Vladimir and Katanić, Jelena and Matić, Sanja and Solujić, Slavica and Vuković, Nenad and Sukdolak, Slobodan",
year = "2014",
abstract = "Eight synthesized 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones were evaluated as in vivo anticoagulants by intraperitoneal application to adult male Wistar rats in order to examine their pharmacological potential, evaluate ther toxicity and propose the mechanism of action. Two of them, 2f and 2a, in concentration of 2mg/kg of body weight, presented remarkable activity (PT=130s; PT=90s) upon seven days of continuous application. The results of rat serum and liver biochemical screening, as well those of histopathological studies, proved the compounds to be non-toxic. Activity of the compounds was further examined on the molecular level. Here, molecular docking studies were performed to position the compounds in relation to the active site of VKORC1 and determine the bioactive conformations. Docking results suggested a non-covalent mode of action during which the proton transfer occurs from Cys135 SH towards 4-carbonyl group of anticoagulant. All crucial interactions for anticoagulant activity were confirmed in generated structure-based 3-D pharmacophore model, consisted of hydrogen bond acceptor and hydrophobic aromatic features, and quantified by a best correlation coefficient of 0.97.",
publisher = "Elsevier",
journal = "European Journal of Pharmaceutical Sciences",
title = "Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.",
number = "1",
volume = "55",
doi = "10.1016/j.ejps.2014.01.004",
pages = "20-35"
}

Stanković, N., Mladenović, M., Mihailović, M., Arambašić Jovanović, J., Uskoković, A., Stanković, V., Mihailović, V., Katanić, J., Matić, S., Solujić, S., Vuković, N.,& Sukdolak, S.. (2014). Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.. in European Journal of Pharmaceutical Sciences
Elsevier., 55(1), 20-35.
https://doi.org/10.1016/j.ejps.2014.01.004

Stanković N, Mladenović M, Mihailović M, Arambašić Jovanović J, Uskoković A, Stanković V, Mihailović V, Katanić J, Matić S, Solujić S, Vuković N, Sukdolak S. Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model.. in European Journal of Pharmaceutical Sciences. 2014;55(1):20-35.
doi:10.1016/j.ejps.2014.01.004 .

Stanković, Nevena, Mladenović, Milan, Mihailović, Mirjana, Arambašić Jovanović, Jelena, Uskoković, Aleksandra, Stanković, Vesna, Mihailović, Vladimir, Katanić, Jelena, Matić, Sanja, Solujić, Slavica, Vuković, Nenad, Sukdolak, Slobodan, "Synthesis and toxicological studies of in vivo anticoagulant activity of novel 3-(1-aminoethylidene)chroman-2,4-diones and 4-hydroxy-3-(1-iminoethyl)-2H-chromen-2-ones combined with a structure-based 3-D pharmacophore model." in European Journal of Pharmaceutical Sciences, 55, no. 1 (2014):20-35,
https://doi.org/10.1016/j.ejps.2014.01.004 . .

TY  - CONF
AU  - Mihailović, Vladimir
AU  - Mišić, Danijela
AU  - Katanić, Jelena
AU  - Mihailović, Mirjana
AU  - Solujić, Slavica
AU  - Stanković, Vesna
AU  - Mladenović, Milan
AU  - Stanković, Nevena
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6142
AB  - Many Gentiono species are known for their pharmaceutical values, such as Gentiono crucioto L, commonly called cross gentian [1]. The dried roots and above-ground parts of G. crucioto are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing beneficial effects in gall and liver diseases [2]. This study using in vivo model investigates hepatoprotective activity of G. crucioto aerial part methanol extract (GCA) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCA (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days before they were treated with CCl4 (1 ml/kg, 1:1 mixture in olive oil) which caused liver injury. Separation, determination and quantification of components in GCA was perform­ed using Dionex Ultimate 3000 UHPLC system equipped with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Pretreatment with GCA dose-dependent­ly and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobarbituric acid-reactive substances, as compared to CCl4 treated group. Histological analyses also show that GCA reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macro-vesicular changes induced by CCl4 in rats. GCA was characterized by the presence of sweroside, swertiamarin, gentiopicrin, loganic acid, isovitexin 4',7-diglucoside, orientin and vitexin, as revealed by UHPLC-DAD-MS and UHPLC-MS/MS analyses. Quantification of targeted compounds in the SRM (selected reaction monitoring) experiment of UHPLC-MS/MS analysis clearly indicated that gentiopicrin (1.067%) was the dominant secoiridoid glycoside in GCA, whereas concentrations of sweroside (0.064%) and swertiamarin (0.033%) were significantly lower.
PB  - Belgrade: Serbian Chemical Society
C3  - Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia
T1  - Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats
SP  - 220
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6142
ER  - 

@conference{
author = "Mihailović, Vladimir and Mišić, Danijela and Katanić, Jelena and Mihailović, Mirjana and Solujić, Slavica and Stanković, Vesna and Mladenović, Milan and Stanković, Nevena",
year = "2013",
abstract = "Many Gentiono species are known for their pharmaceutical values, such as Gentiono crucioto L, commonly called cross gentian [1]. The dried roots and above-ground parts of G. crucioto are consumed in the Balkan region as herbal tea or a medicinal wine for loss of appetite, as a stomachic and component in preparations showing beneficial effects in gall and liver diseases [2]. This study using in vivo model investigates hepatoprotective activity of G. crucioto aerial part methanol extract (GCA) against carbon tetrachloride-induced liver injury in rats. Wistar rats were orally pretreated with GCA (100, 200, and 400 mg/kg) and silymarin (100 mg/kg) for seven days before they were treated with CCl4 (1 ml/kg, 1:1 mixture in olive oil) which caused liver injury. Separation, determination and quantification of components in GCA was perform­ed using Dionex Ultimate 3000 UHPLC system equipped with a diode array detector (DAD) and connected to a triple-quadrupole mass spectrometer. Pretreatment with GCA dose-dependent­ly and significantly (p < 0.001) decreased levels of serum transaminases, alkaline phosphatase and total bilirubin, whereas an increase was found in the level of total protein compared with CCl4-treated group. In the liver tissue antioxidant studies, we found a significant increase in the levels of catalase, superoxide dismutase and reduced glutathione, whereas there was marked reduction in the levels of thiobarbituric acid-reactive substances, as compared to CCl4 treated group. Histological analyses also show that GCA reduced the incidence of liver lesions including necrosis, ballooning degeneration and micro- and macro-vesicular changes induced by CCl4 in rats. GCA was characterized by the presence of sweroside, swertiamarin, gentiopicrin, loganic acid, isovitexin 4',7-diglucoside, orientin and vitexin, as revealed by UHPLC-DAD-MS and UHPLC-MS/MS analyses. Quantification of targeted compounds in the SRM (selected reaction monitoring) experiment of UHPLC-MS/MS analysis clearly indicated that gentiopicrin (1.067%) was the dominant secoiridoid glycoside in GCA, whereas concentrations of sweroside (0.064%) and swertiamarin (0.033%) were significantly lower.",
publisher = "Belgrade: Serbian Chemical Society",
journal = "Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia",
title = "Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats",
pages = "220",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6142"
}

Mihailović, V., Mišić, D., Katanić, J., Mihailović, M., Solujić, S., Stanković, V., Mladenović, M.,& Stanković, N.. (2013). Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats. in Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia
Belgrade: Serbian Chemical Society., 220.
https://hdl.handle.net/21.15107/rcub_ibiss_6142

Mihailović V, Mišić D, Katanić J, Mihailović M, Solujić S, Stanković V, Mladenović M, Stanković N. Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats. in Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia. 2013;:220.
https://hdl.handle.net/21.15107/rcub_ibiss_6142 .

Mihailović, Vladimir, Mišić, Danijela, Katanić, Jelena, Mihailović, Mirjana, Solujić, Slavica, Stanković, Vesna, Mladenović, Milan, Stanković, Nevena, "Phytochemical profiling by UHPLC-DAD/±HESI-MS/MS analyzes and hepatoprotective activity of Gentiana cruciata L. against CCl4 induced liver injury in Wistar rats" in Book of abstracts: 8th International Conference of the Chemical Societies of the South-East European Countries: ICOSECS 8; 2013 Jun 27-29; Belgrade, Serbia (2013):220,
https://hdl.handle.net/21.15107/rcub_ibiss_6142 .

TY  - JOUR
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Vidaković, Melita
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Stanković, Nevena
AU  - Mihailović, Mirjana
PY  - 2013
UR  - http://www.sciencedirect.com/science/article/pii/S1383571813001836
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3188
AB  - The present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000. mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500. mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12. h prior to administration of 100. mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity. © 2013 Elsevier B.V.
PB  - Elsevier
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury
IS  - 2
VL  - 755
DO  - 10.1016/j.mrgentox.2013.03.011
SP  - 81
EP  - 89
ER  - 

@article{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Vidaković, Melita and Grdović, Nevena and Dinić, Svetlana and Solujić, Slavica and Mladenović, Milan and Stanković, Nevena and Mihailović, Mirjana",
year = "2013",
abstract = "The present study was undertaken to investigate the hepatoprotective effect of the methanol extract of Cotinus coggygria Scop. in rats exposed to the hepatotoxic compound pyrogallol. Assessed with the alkaline version of the comet assay, 1000 and 2000. mg/kg body weight (bw) of the extract showed a low level of genotoxicity, while 500. mg/kg bw of the extract showed no genotoxic potential. Quantitative HPLC analysis of phenolic acids and flavonoids in the methanol extract of C. coggygria showed that myricetin was a major component. To test the hepatoprotective effect, a non-genotoxic dose of the C. coggygria extract and an equivalent amount of synthetic myricetin, as present in the extract, were applied either 2 or 12. h prior to administration of 100. mg/kg bw of pyrogallol. The extract and myricetin promoted restoration of hepatic function by significantly reducing pyrogallol-induced elevation in the serum enzymes AST, ALT, ALP and in total bilirubin. As measured by the decrease in total score and tail moment, the DNA damage in liver was also reduced by the extract and by myricetin. Our results suggest that pro-surviving Akt activity and STAT3 protein expression play important roles in decreasing DNA damage and in mediating hepatic protection by the extract. These results suggest that myricetin, as a major component in the extract, is responsible for the antigenotoxic and hepatoprotective properties of the methanol extract of C. coggygria against pyrogallol-induced toxicity. © 2013 Elsevier B.V.",
publisher = "Elsevier",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury",
number = "2",
volume = "755",
doi = "10.1016/j.mrgentox.2013.03.011",
pages = "81-89"
}

Matić, S., Stanić, S., Bogojević, D., Vidaković, M., Grdović, N., Dinić, S., Solujić, S., Mladenović, M., Stanković, N.,& Mihailović, M.. (2013). Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier., 755(2), 81-89.
https://doi.org/10.1016/j.mrgentox.2013.03.011

Matić S, Stanić S, Bogojević D, Vidaković M, Grdović N, Dinić S, Solujić S, Mladenović M, Stanković N, Mihailović M. Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2013;755(2):81-89.
doi:10.1016/j.mrgentox.2013.03.011 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Vidaković, Melita, Grdović, Nevena, Dinić, Svetlana, Solujić, Slavica, Mladenović, Milan, Stanković, Nevena, Mihailović, Mirjana, "Methanol extract from the stem of Cotinus coggygria Scop., and its major bioactive phytochemical constituent myricetin modulate pyrogallol-induced DNA damage and liver injury" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 755, no. 2 (2013):81-89,
https://doi.org/10.1016/j.mrgentox.2013.03.011 . .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
AU  - Matić, Sanja
AU  - Nićiforović, Neda
AU  - Mihailović, Vladimir
AU  - Mašković, Pavle
AU  - Vrvić, Miroslav M.
AU  - Solujić, Slavica
PY  - 2012
UR  - https://www.sciencedirect.com/science/article/pii/S0223523412002887?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3201
AB  - The objective of this study was to investigate in vitro and in vivo anticoagulant activity of sixteen 4-hydroxycoumarin derivatives bearing polar C-3 scaffolds. The activity was evaluated by measuring prothrombin time. Enhanced anticoagulant activity in vitro was observed for all tested compounds. Upon successive administration of 0.5 mg/kg of body weight to adult Wistar rats, over a period of five days, four derivatives (2b, 4c, 5c and 9c) presented anticoagulant activity in vivo. The most active compound was 2b, with PT = 30.0 s. Low or non-toxic effects in vivo were determined based on the catalytic activity of liver enzymes and the concentration of bilirubin, iron and proteins. Metabolic pathways of the most active compounds in vivo were determined after GC/MS analysis of collected rat urine samples. The excretion occurs by glucuronidation of 7-hydroxy forms of tested derivatives. In vivo results were described using PLS-based CoMFA and CoMSIA 3D-QSAR studies, which showed CoMFA-SE (q2 = 0.738) and CoMSIA-SEA (q2 = 0.763) to be the statistically most relevant models. Furthermore, molecular docking and DFT mechanistic studies performed on the rat VKORC1 homology model revealed interactions between the 4-OH coumarin group in the form of phenolic anion and the Cys135 catalytic site in the transition state.
T2  - European Journal of Medicinal Chemistry
T1  - Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants
VL  - 54
DO  - 10.1016/J.EJMECH.2012.04.036
SP  - 144
EP  - 158
ER  - 

@article{
author = "Mladenović, Milan and Mihailović, Mirjana and Bogojević, Desanka and Vuković, Nenad and Sukdolak, Slobodan and Matić, Sanja and Nićiforović, Neda and Mihailović, Vladimir and Mašković, Pavle and Vrvić, Miroslav M. and Solujić, Slavica",
year = "2012",
abstract = "The objective of this study was to investigate in vitro and in vivo anticoagulant activity of sixteen 4-hydroxycoumarin derivatives bearing polar C-3 scaffolds. The activity was evaluated by measuring prothrombin time. Enhanced anticoagulant activity in vitro was observed for all tested compounds. Upon successive administration of 0.5 mg/kg of body weight to adult Wistar rats, over a period of five days, four derivatives (2b, 4c, 5c and 9c) presented anticoagulant activity in vivo. The most active compound was 2b, with PT = 30.0 s. Low or non-toxic effects in vivo were determined based on the catalytic activity of liver enzymes and the concentration of bilirubin, iron and proteins. Metabolic pathways of the most active compounds in vivo were determined after GC/MS analysis of collected rat urine samples. The excretion occurs by glucuronidation of 7-hydroxy forms of tested derivatives. In vivo results were described using PLS-based CoMFA and CoMSIA 3D-QSAR studies, which showed CoMFA-SE (q2 = 0.738) and CoMSIA-SEA (q2 = 0.763) to be the statistically most relevant models. Furthermore, molecular docking and DFT mechanistic studies performed on the rat VKORC1 homology model revealed interactions between the 4-OH coumarin group in the form of phenolic anion and the Cys135 catalytic site in the transition state.",
journal = "European Journal of Medicinal Chemistry",
title = "Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants",
volume = "54",
doi = "10.1016/J.EJMECH.2012.04.036",
pages = "144-158"
}

Mladenović, M., Mihailović, M., Bogojević, D., Vuković, N., Sukdolak, S., Matić, S., Nićiforović, N., Mihailović, V., Mašković, P., Vrvić, M. M.,& Solujić, S.. (2012). Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants. in European Journal of Medicinal Chemistry, 54, 144-158.
https://doi.org/10.1016/J.EJMECH.2012.04.036

Mladenović M, Mihailović M, Bogojević D, Vuković N, Sukdolak S, Matić S, Nićiforović N, Mihailović V, Mašković P, Vrvić MM, Solujić S. Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants. in European Journal of Medicinal Chemistry. 2012;54:144-158.
doi:10.1016/J.EJMECH.2012.04.036 .

Mladenović, Milan, Mihailović, Mirjana, Bogojević, Desanka, Vuković, Nenad, Sukdolak, Slobodan, Matić, Sanja, Nićiforović, Neda, Mihailović, Vladimir, Mašković, Pavle, Vrvić, Miroslav M., Solujić, Slavica, "Biochemical and pharmacological evaluation of 4-hydroxychromen-2-ones bearing polar C-3 substituents as anticoagulants" in European Journal of Medicinal Chemistry, 54 (2012):144-158,
https://doi.org/10.1016/J.EJMECH.2012.04.036 . .

TY  - JOUR
AU  - Matić, Sanja
AU  - Stanić, Snežana
AU  - Bogojević, Desanka
AU  - Vidaković, Melita
AU  - Grdović, Nevena
AU  - Arambašić Jovanović, Jelena
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Poznanović, Goran
AU  - Solujić, Slavica
AU  - Mladenović, Milan
AU  - Marković, Jelena
AU  - Mihailović, Mirjana
PY  - 2011
UR  - http://www.nrcresearchpress.com/doi/10.1139/y11-043#.XBD8lM0o-Uk
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3204
AB  - To examine the protective potential of the Cotinus coggygria Scop. methanol extract, Wistar rats were treated with the hepatotoxic compound pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The ability of the extract to counteract the oxidative stress was examined in rats that were injected with the extract intraperitoneally (500 mg·(kg body weight)(-1)) either 2 or 12 h before the pyrogallol treatment. The extract possesses a reducing activity in vitro and an ability to chelate the ferrous ion both in vivo and in vitro. Application of the extract prior to pyrogallol treatment led to a decrease in the levels of thiobarbituric acid-reactive substances, aspartate aminotransferase, and alanine aminotransferase, increased activities of antioxidant enzymes and attenuation of DNA damage, as well as increased Akt activity and inhibition of NF-κB protein expression. Treatment with the extract 12 h prior to pyrogallol administration was more effective in suppressing pyrogallol-induced oxidative damage than the 2 h pretreatment. Extract administration promoted an increase in acute phase reactants haptoglobin and α(2)-macroglobulin that was short of a full-fledged acute phase response. Administration of the extract considerably improved the markers of oxidative stress, thus revealing a potential hepatoprotective activity. Our results suggest that Akt activation, NF-κB inhibition, and induction of the acute phase play important roles in mediating hepatic protection by the extract. The greater effectiveness of the 12 h pretreatment with extract points to the important role that preconditioning assumes in improving resistance to subsequent exposure to oxidative stress.
PB  - NRC Research Press; Canadian Science Publishing; National Research Council of Canada
PB  - © 2011 by NRC Research Press
T2  - Canadian Journal of Physiology and Pharmacology
T1  - Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats
IS  - 6
VL  - 89
DO  - 10.1139/y11-043
SP  - 401
EP  - 411
ER  - 

@article{
author = "Matić, Sanja and Stanić, Snežana and Bogojević, Desanka and Vidaković, Melita and Grdović, Nevena and Arambašić Jovanović, Jelena and Dinić, Svetlana and Uskoković, Aleksandra and Poznanović, Goran and Solujić, Slavica and Mladenović, Milan and Marković, Jelena and Mihailović, Mirjana",
year = "2011",
abstract = "To examine the protective potential of the Cotinus coggygria Scop. methanol extract, Wistar rats were treated with the hepatotoxic compound pyrogallol, which possesses a potent ability to generate free radicals and induce oxidative stress. The ability of the extract to counteract the oxidative stress was examined in rats that were injected with the extract intraperitoneally (500 mg·(kg body weight)(-1)) either 2 or 12 h before the pyrogallol treatment. The extract possesses a reducing activity in vitro and an ability to chelate the ferrous ion both in vivo and in vitro. Application of the extract prior to pyrogallol treatment led to a decrease in the levels of thiobarbituric acid-reactive substances, aspartate aminotransferase, and alanine aminotransferase, increased activities of antioxidant enzymes and attenuation of DNA damage, as well as increased Akt activity and inhibition of NF-κB protein expression. Treatment with the extract 12 h prior to pyrogallol administration was more effective in suppressing pyrogallol-induced oxidative damage than the 2 h pretreatment. Extract administration promoted an increase in acute phase reactants haptoglobin and α(2)-macroglobulin that was short of a full-fledged acute phase response. Administration of the extract considerably improved the markers of oxidative stress, thus revealing a potential hepatoprotective activity. Our results suggest that Akt activation, NF-κB inhibition, and induction of the acute phase play important roles in mediating hepatic protection by the extract. The greater effectiveness of the 12 h pretreatment with extract points to the important role that preconditioning assumes in improving resistance to subsequent exposure to oxidative stress.",
publisher = "NRC Research Press; Canadian Science Publishing; National Research Council of Canada, © 2011 by NRC Research Press",
journal = "Canadian Journal of Physiology and Pharmacology",
title = "Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats",
number = "6",
volume = "89",
doi = "10.1139/y11-043",
pages = "401-411"
}

Matić, S., Stanić, S., Bogojević, D., Vidaković, M., Grdović, N., Arambašić Jovanović, J., Dinić, S., Uskoković, A., Poznanović, G., Solujić, S., Mladenović, M., Marković, J.,& Mihailović, M.. (2011). Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats. in Canadian Journal of Physiology and Pharmacology
NRC Research Press; Canadian Science Publishing; National Research Council of Canada., 89(6), 401-411.
https://doi.org/10.1139/y11-043

Matić S, Stanić S, Bogojević D, Vidaković M, Grdović N, Arambašić Jovanović J, Dinić S, Uskoković A, Poznanović G, Solujić S, Mladenović M, Marković J, Mihailović M. Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats. in Canadian Journal of Physiology and Pharmacology. 2011;89(6):401-411.
doi:10.1139/y11-043 .

Matić, Sanja, Stanić, Snežana, Bogojević, Desanka, Vidaković, Melita, Grdović, Nevena, Arambašić Jovanović, Jelena, Dinić, Svetlana, Uskoković, Aleksandra, Poznanović, Goran, Solujić, Slavica, Mladenović, Milan, Marković, Jelena, Mihailović, Mirjana, "Extract of the plant Cotinus coggygria Scop. attenuates pyrogallol-induced hepatic oxidative stress in Wistar rats" in Canadian Journal of Physiology and Pharmacology, 89, no. 6 (2011):401-411,
https://doi.org/10.1139/y11-043 . .

TY  - JOUR
AU  - Mladenović, Milan
AU  - Mihailović, Mirjana
AU  - Bogojević, Desanka
AU  - Matić, Sanja
AU  - Nićiforović, Neda
AU  - Mihailović, Vladimir
AU  - Vuković, Nenad
AU  - Sukdolak, Slobodan
AU  - Solujić, Slavica
PY  - 2011
UR  - http://www.scopus.com/inward/record.url?eid=2-s2.0-79957795403&partnerID=tZOtx3y1
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3207
AB  - The series of fifteen synthesized 4-hydroxycoumarin derivatives was subjected to antioxidant activity evaluation in vitro, through total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl radical, lipid peroxide scavenging and chelating activity. The highest activity was detected during the radicals scavenging, with 2b, 6b, 2c, and 4c noticed as the most active. The antioxidant activity was further quantified by the quantitative structure-activity relationships (QSAR) studies. For this purpose, the structures were optimized using Paramethric Method 6 (PM6) semi-empirical and Density Functional Theory (DFT) B3LYP methods. Bond dissociation enthalpies of coumarin 4-OH, Natural Bond Orbital (NBO) gained hybridization of the oxygen, acidity of the hydrogen atom and various molecular descriptors obtained, were correlated with biological activity, after which we designed 20 new antioxidant structures, using the most favorable structural motifs, with much improved predicted activity in vitro.
T2  - International Journal of Molecular Sciences
T1  - In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.
IS  - 5
VL  - 12
DO  - 10.3390/ijms12052822
SP  - 2822
EP  - 2841
ER  - 

@article{
author = "Mladenović, Milan and Mihailović, Mirjana and Bogojević, Desanka and Matić, Sanja and Nićiforović, Neda and Mihailović, Vladimir and Vuković, Nenad and Sukdolak, Slobodan and Solujić, Slavica",
year = "2011",
abstract = "The series of fifteen synthesized 4-hydroxycoumarin derivatives was subjected to antioxidant activity evaluation in vitro, through total antioxidant capacity, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), hydroxyl radical, lipid peroxide scavenging and chelating activity. The highest activity was detected during the radicals scavenging, with 2b, 6b, 2c, and 4c noticed as the most active. The antioxidant activity was further quantified by the quantitative structure-activity relationships (QSAR) studies. For this purpose, the structures were optimized using Paramethric Method 6 (PM6) semi-empirical and Density Functional Theory (DFT) B3LYP methods. Bond dissociation enthalpies of coumarin 4-OH, Natural Bond Orbital (NBO) gained hybridization of the oxygen, acidity of the hydrogen atom and various molecular descriptors obtained, were correlated with biological activity, after which we designed 20 new antioxidant structures, using the most favorable structural motifs, with much improved predicted activity in vitro.",
journal = "International Journal of Molecular Sciences",
title = "In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.",
number = "5",
volume = "12",
doi = "10.3390/ijms12052822",
pages = "2822-2841"
}

Mladenović, M., Mihailović, M., Bogojević, D., Matić, S., Nićiforović, N., Mihailović, V., Vuković, N., Sukdolak, S.,& Solujić, S.. (2011). In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.. in International Journal of Molecular Sciences, 12(5), 2822-2841.
https://doi.org/10.3390/ijms12052822

Mladenović M, Mihailović M, Bogojević D, Matić S, Nićiforović N, Mihailović V, Vuković N, Sukdolak S, Solujić S. In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies.. in International Journal of Molecular Sciences. 2011;12(5):2822-2841.
doi:10.3390/ijms12052822 .

Mladenović, Milan, Mihailović, Mirjana, Bogojević, Desanka, Matić, Sanja, Nićiforović, Neda, Mihailović, Vladimir, Vuković, Nenad, Sukdolak, Slobodan, Solujić, Slavica, "In vitro antioxidant activity of selected 4-hydroxy-chromene-2-one derivatives-SAR, QSAR and DFT studies." in International Journal of Molecular Sciences, 12, no. 5 (2011):2822-2841,
https://doi.org/10.3390/ijms12052822 . .