TY  - CONF
AU  - Dubljević, Olga
AU  - Pavković, Željko
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5845
AB  - The molecular mechanisms behind non-motor symptoms of Parkinson’s disease are poorly understood. In literature, the connection between neuroinflammation and depressive symptoms was observed in PD patients, however the implications of such findings are limited due to cerebrospinal fluid and blood derivates being the only obtainable samples for analysis. The aim of this preliminary study was to assess neuroinflammation, memory deficits and depression like symptoms associated with PD and to detect related proinflammatory mediators using a transgenic A53T mouse model. The transgene consists of the human α-synuclein sequence with the A53T mutation driven by the mouse prion protein (PrP) promoter resulting in overexpression of human α-synuclein in key structures associated with PD. Although it was determined that microglial activation is increased in these mice, the specific cytokine profile driving the inflammation has not been characterized in literature. We have examined the expression of three key inflammatory mediators (tumor necrosis factor alpha (TNFα), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) described as markers associated with the microglial pro-inflammatory phenotype in the prefrontal cortex, hippocampus, and striatum of 8 male 6 months old A53T mice. Additionally, depressive like symptoms and anhedonia were assessed using Tail Suspension Test and Sucrose Preference Test respectively, while cognitive status and memory deficits were assessed using Novel Object Recognition Test. Non-transgenic littermates were used as controls. Obtained results depict a characteristic behavioral and pro-inflammatory profile in the transgenic animals. The implications of the obtained findings and the potential for using the A53T model as a tool for studying non-motor features of PD are further discussed.
PB  - Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria
T1  - Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5845
ER  - 

@conference{
author = "Dubljević, Olga and Pavković, Željko and Pešić, Vesna",
year = "2023",
abstract = "The molecular mechanisms behind non-motor symptoms of Parkinson’s disease are poorly understood. In literature, the connection between neuroinflammation and depressive symptoms was observed in PD patients, however the implications of such findings are limited due to cerebrospinal fluid and blood derivates being the only obtainable samples for analysis. The aim of this preliminary study was to assess neuroinflammation, memory deficits and depression like symptoms associated with PD and to detect related proinflammatory mediators using a transgenic A53T mouse model. The transgene consists of the human α-synuclein sequence with the A53T mutation driven by the mouse prion protein (PrP) promoter resulting in overexpression of human α-synuclein in key structures associated with PD. Although it was determined that microglial activation is increased in these mice, the specific cytokine profile driving the inflammation has not been characterized in literature. We have examined the expression of three key inflammatory mediators (tumor necrosis factor alpha (TNFα), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) described as markers associated with the microglial pro-inflammatory phenotype in the prefrontal cortex, hippocampus, and striatum of 8 male 6 months old A53T mice. Additionally, depressive like symptoms and anhedonia were assessed using Tail Suspension Test and Sucrose Preference Test respectively, while cognitive status and memory deficits were assessed using Novel Object Recognition Test. Non-transgenic littermates were used as controls. Obtained results depict a characteristic behavioral and pro-inflammatory profile in the transgenic animals. The implications of the obtained findings and the potential for using the A53T model as a tool for studying non-motor features of PD are further discussed.",
publisher = "Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria",
title = "Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5845"
}

Dubljević, O., Pavković, Ž.,& Pešić, V.. (2023). Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model. in FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria
Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5845

Dubljević O, Pavković Ž, Pešić V. Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model. in FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5845 .

Dubljević, Olga, Pavković, Željko, Pešić, Vesna, "Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model" in FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5845 .

TY  - CONF
AU  - Dubljević, Olga
AU  - Pavković, Željko
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5844
AB  - The molecular mechanisms behind non-motor symptoms of Parkinson’s disease are poorly understood. In literature, the connection between neuroinflammation and depressive symptoms was observed in PD patients, however the implications of such findings are limited due to cerebrospinal fluid and blood derivates being the only obtainable samples for analysis. The aim of this preliminary study was to assess neuroinflammation, memory deficits and depression like symptoms associated with PD and to detect related proinflammatory mediators using a transgenic A53T mouse model. The transgene consists of the human α-synuclein sequence with the A53T mutation driven by the mouse prion protein (PrP) promoter resulting in overexpression of human α-synuclein in key structures associated with PD. Although it was determined that microglial activation is increased in these mice, the specific cytokine profile driving the inflammation has not been characterized in literature. We have examined the expression of three key inflammatory mediators (tumor necrosis factor alpha (TNFα), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) described as markers associated with the microglial pro-inflammatory phenotype in the prefrontal cortex, hippocampus, and striatum of 8 male 6 months old A53T mice. Additionally, depressive like symptoms and anhedonia were assessed using Tail Suspension Test and Sucrose Preference Test respectively, while cognitive status and memory deficits were assessed using Novel Object Recognition Test. Non-transgenic littermates were used as controls. Obtained results depict a characteristic behavioral and pro-inflammatory profile in the transgenic animals. The implications of the obtained findings and the potential for using the A53T model as a tool for studying non-motor features of PD are further discussed.
PB  - Federation of European Neuroscience Societies
C3  - FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria
T1  - Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5844
ER  - 

@conference{
author = "Dubljević, Olga and Pavković, Željko and Pešić, Vesna",
year = "2023",
abstract = "The molecular mechanisms behind non-motor symptoms of Parkinson’s disease are poorly understood. In literature, the connection between neuroinflammation and depressive symptoms was observed in PD patients, however the implications of such findings are limited due to cerebrospinal fluid and blood derivates being the only obtainable samples for analysis. The aim of this preliminary study was to assess neuroinflammation, memory deficits and depression like symptoms associated with PD and to detect related proinflammatory mediators using a transgenic A53T mouse model. The transgene consists of the human α-synuclein sequence with the A53T mutation driven by the mouse prion protein (PrP) promoter resulting in overexpression of human α-synuclein in key structures associated with PD. Although it was determined that microglial activation is increased in these mice, the specific cytokine profile driving the inflammation has not been characterized in literature. We have examined the expression of three key inflammatory mediators (tumor necrosis factor alpha (TNFα), interleukin-1beta (IL-1β) and interleukin-6 (IL-6) described as markers associated with the microglial pro-inflammatory phenotype in the prefrontal cortex, hippocampus, and striatum of 8 male 6 months old A53T mice. Additionally, depressive like symptoms and anhedonia were assessed using Tail Suspension Test and Sucrose Preference Test respectively, while cognitive status and memory deficits were assessed using Novel Object Recognition Test. Non-transgenic littermates were used as controls. Obtained results depict a characteristic behavioral and pro-inflammatory profile in the transgenic animals. The implications of the obtained findings and the potential for using the A53T model as a tool for studying non-motor features of PD are further discussed.",
publisher = "Federation of European Neuroscience Societies",
journal = "FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria",
title = "Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5844"
}

Dubljević, O., Pavković, Ž.,& Pešić, V.. (2023). Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model. in FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria
Federation of European Neuroscience Societies., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_5844

Dubljević O, Pavković Ž, Pešić V. Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model. in FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria. 2023;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_5844 .

Dubljević, Olga, Pavković, Željko, Pešić, Vesna, "Neuroinflammation associated with non-motor symptoms in Parkinson’s disease- insights from the A53T mouse model" in FENS-Hertie Winter School: “Neuro-immune interactions in health and disease”; 2023 Jan 29 - Feb 4; Obergurgl, Austria (2023):32,
https://hdl.handle.net/21.15107/rcub_ibiss_5844 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5842
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5842
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main 
cause of dementia in the elderly worldwide. It is also well-established that the 
prevalence and severity of AD is greater in women than in men, suggesting that sex is 
a crucial variable in disease heterogeneity.
Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like 
amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were 
assessed using novel object recognition (NOR) and relocation test (NOL), 
respectively, while for depressive-like behavior tail-suspension test (TST) was used. 
Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were 
tested at the age of 9 months.
Memory impairments were evident in both WT and APPNL-G-F females in comparison 
to their male littermates. In NOL as a spatial variation of NOR, the discrimination 
index was decreased, but not in NOR as such. Furthermore, the decrease in total active 
immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. 
male mice suggesting more prominent depressive-like behavior as well. Examined 
parameters had similar pattern in WT and APPNL-G-F mice of both sexes. 
The results suggest prominent sex-biased differences in behavior of males and females 
in this particular model and support its validity for further studies revealing the impact 
of sex to behavioral deficits.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5842"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:110.
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):110,
https://hdl.handle.net/21.15107/rcub_ibiss_5842 .

TY  - CONF
AU  - Milovanović, Nikola
AU  - Jovanović Macura, Irena
AU  - Tešić, Vesna
AU  - Pavković, Željko
AU  - Perović, Milka
AU  - Pešić, Vesna
AU  - Ćirić, Jelena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5843
AB  - Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade
C3  - 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5843
ER  - 

@conference{
author = "Milovanović, Nikola and Jovanović Macura, Irena and Tešić, Vesna and Pavković, Željko and Perović, Milka and Pešić, Vesna and Ćirić, Jelena",
year = "2023",
abstract = "Alzheimer’s disease (AD) is progressive age-associated brain disorder and the main cause of dementia in the elderly worldwide. It is also well-established that the prevalence and severity of AD is greater in women than in men, suggesting that sex is a crucial variable in disease heterogeneity.Sex-biased differences in behavioral parameters related to cognition and depressive like behavior were examined in novel, state-of-the-art mouse model of AD-like amyloidosis, APPNL-G-F knock-in (KI) mice. Nonspatial and spatial memory were assessed using novel object recognition (NOR) and relocation test (NOL), respectively, while for depressive-like behavior tail-suspension test (TST) was used. Male and female APPNL-G-F mice and their non-APPNL-G-F KI littermates (WT) were tested at the age of 9 months.Memory impairments were evident in both WT and APPNL-G-F females in comparison to their male littermates. In NOL as a spatial variation of NOR, the discrimination index was decreased, but not in NOR as such. Furthermore, the decrease in total active immobility time in TST test was also detected in female WT and APPNL-G-F mice vs. male mice suggesting more prominent depressive-like behavior as well. Examined parameters had similar pattern in WT and APPNL-G-F mice of both sexes. The results suggest prominent sex-biased differences in behavior of males and females in this particular model and support its validity for further studies revealing the impact of sex to behavioral deficits.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade",
journal = "8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5843"
}

Milovanović, N., Jovanović Macura, I., Tešić, V., Pavković, Ž., Perović, M., Pešić, V.,& Ćirić, J.. (2023). The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković" – National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_5843

Milovanović N, Jovanović Macura I, Tešić V, Pavković Ž, Perović M, Pešić V, Ćirić J. The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease. in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

Milovanović, Nikola, Jovanović Macura, Irena, Tešić, Vesna, Pavković, Željko, Perović, Milka, Pešić, Vesna, Ćirić, Jelena, "The impact of sex on behavioral deficits in APP knock-in mouse model of Alzheimer's disease" in 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5843 .

TY  - CONF
AU  - Pavković, Željko
AU  - Potrebić, Milica
AU  - Dubljević, Olga
AU  - Srbovan, Maja
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5832
AB  - Adolescence is a period when alcohol abuse is more likely to occur. Use of isoflurane
(a volatile anesthetic) in adolescence has been experimentally shown to alter
responses to ethanol, implying that general exposure to anesthetics during adolescence
may be an environmental risk factor contributing to increased susceptibility to alcohol
use disorder (AUD). Our previous work showed that propofol (intravenous anesthetic)
anesthesia exposure (PAE) in adolescence favors risk-taking and preference for
novel/intense stimuli. However, its influence on alcohol consumption hasn’t been
examined. Therefore, we aimed to investigate whether PAE in adolescence alters
alcohol consumption in alcohol-naïve rats.
Male Wistar rats aged 35 and 50 postnatal days (early-to-mid and mid-to-late
adolescence, respectively) were injected with anesthetic dose of propofol emulsion
(75mg/kg) or saline, separated into individual cages and exposed to two-bottle choice
paradigm (allowing free access to food and two bottles, one containing 2,5% ethanol
in 1% sucrose solution and the other containing tap water). Liquid consumption was
measured for 4 constitutive days. Obtained data showed no preference for alcohol in
all analyzed experimental groups. Moreover, the tendency for alcohol intake was
significantly reduced during first 24h after PAE in early-to-mid adolescent rats.
Overall, our study shows that PAE doesn’t increase voluntary alcohol intake in
peripubertal rats used as a rodent model of adolescence. The obtained findings do not
support the view that exposure to general anesthetics in adolescence is a risk factor for
AUD and suggest that a detailed analysis of the range of anesthetics is needed before
generalizing the data.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Propofol reduces the tendency for alcohol consumption in adolescent rats
SP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5832
ER  - 

@conference{
author = "Pavković, Željko and Potrebić, Milica and Dubljević, Olga and Srbovan, Maja and Pešić, Vesna",
year = "2023",
abstract = "Adolescence is a period when alcohol abuse is more likely to occur. Use of isoflurane
(a volatile anesthetic) in adolescence has been experimentally shown to alter
responses to ethanol, implying that general exposure to anesthetics during adolescence
may be an environmental risk factor contributing to increased susceptibility to alcohol
use disorder (AUD). Our previous work showed that propofol (intravenous anesthetic)
anesthesia exposure (PAE) in adolescence favors risk-taking and preference for
novel/intense stimuli. However, its influence on alcohol consumption hasn’t been
examined. Therefore, we aimed to investigate whether PAE in adolescence alters
alcohol consumption in alcohol-naïve rats.
Male Wistar rats aged 35 and 50 postnatal days (early-to-mid and mid-to-late
adolescence, respectively) were injected with anesthetic dose of propofol emulsion
(75mg/kg) or saline, separated into individual cages and exposed to two-bottle choice
paradigm (allowing free access to food and two bottles, one containing 2,5% ethanol
in 1% sucrose solution and the other containing tap water). Liquid consumption was
measured for 4 constitutive days. Obtained data showed no preference for alcohol in
all analyzed experimental groups. Moreover, the tendency for alcohol intake was
significantly reduced during first 24h after PAE in early-to-mid adolescent rats.
Overall, our study shows that PAE doesn’t increase voluntary alcohol intake in
peripubertal rats used as a rodent model of adolescence. The obtained findings do not
support the view that exposure to general anesthetics in adolescence is a risk factor for
AUD and suggest that a detailed analysis of the range of anesthetics is needed before
generalizing the data.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Propofol reduces the tendency for alcohol consumption in adolescent rats",
pages = "70",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5832"
}

Pavković, Ž., Potrebić, M., Dubljević, O., Srbovan, M.,& Pešić, V.. (2023). Propofol reduces the tendency for alcohol consumption in adolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 70.
https://hdl.handle.net/21.15107/rcub_ibiss_5832

Pavković Ž, Potrebić M, Dubljević O, Srbovan M, Pešić V. Propofol reduces the tendency for alcohol consumption in adolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:70.
https://hdl.handle.net/21.15107/rcub_ibiss_5832 .

Pavković, Željko, Potrebić, Milica, Dubljević, Olga, Srbovan, Maja, Pešić, Vesna, "Propofol reduces the tendency for alcohol consumption in adolescent rats" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):70,
https://hdl.handle.net/21.15107/rcub_ibiss_5832 .

TY  - CONF
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Dubljević, Olga
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5831
AB  - Aim: Microglia are macrophages of the brain with a fundamental role in neuronal
homeostasis. Stress-induced activation of microglia leads to rapid morphological
changes and it is implicated that these alternations could mediate different aspects of
early-life stress psychopathologies. We aimed to examine morphological response of
hippocampal microglia to social isolation using peripubertal rats as a rodent model of
adolescence.
Methods: Male peripubertal Wistar rats were randomly selected for group-housing
(GH; n=3 per cage) or single-housing (SH; n=1 per cage) at postnatal day 29. After
1st and 2nd week of defined housing animals῾ brains were sampled, and used for
microglia iba-1 fluorescent labelling and morphology analysis: one filed per section
per animal (n=4 animals per group) in hippocampal dentate gyrus (HDG). Data for
microglia cell shape (FracLac plugin, ImageJ) and cell ramification (Analyze Skeleton
plugin, ImageJ) were collected from single cells in photomicrograph visualized with a
confocal microscope (Leica Microsystems).
Results: Compared to GH peers, SH rats had lower microglial cell density in the HDG
after the 1st week of defined housing, which was not evident after the 2nd week. Other
parameters of microglia shape and ramification showed no difference between the
groups in neither of the two time points examined.
Conclusions: Obtained results showed that change in the cell density in the HDG was
the only and transient change in microglia morphology due to the social isolation
during adolescence. Further examination of other stress-related brain regions is
needed to define in depth the relationship between the microglia phenotype and earlylife
stress conditions.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Microglial morphological response to the lack of direct social contact in periadolescent rats
SP  - 108
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5831
ER  - 

@conference{
author = "Potrebić, Milica and Pavković, Željko and Srbovan, Maja and Dubljević, Olga and Pešić, Vesna",
year = "2023",
abstract = "Aim: Microglia are macrophages of the brain with a fundamental role in neuronal
homeostasis. Stress-induced activation of microglia leads to rapid morphological
changes and it is implicated that these alternations could mediate different aspects of
early-life stress psychopathologies. We aimed to examine morphological response of
hippocampal microglia to social isolation using peripubertal rats as a rodent model of
adolescence.
Methods: Male peripubertal Wistar rats were randomly selected for group-housing
(GH; n=3 per cage) or single-housing (SH; n=1 per cage) at postnatal day 29. After
1st and 2nd week of defined housing animals῾ brains were sampled, and used for
microglia iba-1 fluorescent labelling and morphology analysis: one filed per section
per animal (n=4 animals per group) in hippocampal dentate gyrus (HDG). Data for
microglia cell shape (FracLac plugin, ImageJ) and cell ramification (Analyze Skeleton
plugin, ImageJ) were collected from single cells in photomicrograph visualized with a
confocal microscope (Leica Microsystems).
Results: Compared to GH peers, SH rats had lower microglial cell density in the HDG
after the 1st week of defined housing, which was not evident after the 2nd week. Other
parameters of microglia shape and ramification showed no difference between the
groups in neither of the two time points examined.
Conclusions: Obtained results showed that change in the cell density in the HDG was
the only and transient change in microglia morphology due to the social isolation
during adolescence. Further examination of other stress-related brain regions is
needed to define in depth the relationship between the microglia phenotype and earlylife
stress conditions.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Microglial morphological response to the lack of direct social contact in periadolescent rats",
pages = "108",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5831"
}

Potrebić, M., Pavković, Ž., Srbovan, M., Dubljević, O.,& Pešić, V.. (2023). Microglial morphological response to the lack of direct social contact in periadolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 108.
https://hdl.handle.net/21.15107/rcub_ibiss_5831

Potrebić M, Pavković Ž, Srbovan M, Dubljević O, Pešić V. Microglial morphological response to the lack of direct social contact in periadolescent rats. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:108.
https://hdl.handle.net/21.15107/rcub_ibiss_5831 .

Potrebić, Milica, Pavković, Željko, Srbovan, Maja, Dubljević, Olga, Pešić, Vesna, "Microglial morphological response to the lack of direct social contact in periadolescent rats" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):108,
https://hdl.handle.net/21.15107/rcub_ibiss_5831 .

TY  - CONF
AU  - Dubljević, Olga
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Potrebić, Milica
AU  - Pešić, Vesna
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5830
AB  - Parkinson's disease (PD) is characterized by both motor and non-motor symptoms.
Current research indicates that neuroinflammation, along with activated glial cells,
represents one of the key factors in the pathogenesis of PD. The aim of this study was
to assess non-motor symptoms of PD as well as the expression of gene markers of
microgliosis, astrogliosis and neuroinflammation in a transgenic A53T mouse model,
which is recommended for studying PD and other synucleinopathies. These transgenic
mice express an A53T missense mutant form of human alpha-synuclein under the
control of the murine prion promoter.
Depression-like symptoms and anhedonia were assessed using Tail Suspension Test
and Sucrose Preference Test respectively, while memory deficits were assessed using
Novel Object Recognition Test along with Object Relocation Test. Gene expression of
ionized calcium-binding adapter molecule (Iba-1), glial fibrillary acidic protein
(GFAP α and δ form) and cytokines associated with the microglial pro-inflammatory
phenotype (TNFα, IL-1β, IL-6) was assessed using RT-PCR. Brain regions of interest
were the prefrontal cortex, hippocampus, and striatum of 6 months old A53T male
mice. Non-transgenic littermates were used as controls. Obtained results suggest that
A53T mice compared to non-transgenic littermates exert 1) the presence of memory
deficits and anhedonia, 2) no difference in the expression of glial activity markers and
pro-inflammatory cytokines in the cortex and striatum, and 3) increased expression of
glial activity markers in the hippocampus, but without significant changes in the
expression of cytokines. Overall, better understanding of the role of active glia in the
model is needed.
PB  - Belgrade: Serbian Neuroscience Society
C3  - Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
T1  - Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease
SP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5830
ER  - 

@conference{
author = "Dubljević, Olga and Pavković, Željko and Srbovan, Maja and Potrebić, Milica and Pešić, Vesna",
year = "2023",
abstract = "Parkinson's disease (PD) is characterized by both motor and non-motor symptoms.
Current research indicates that neuroinflammation, along with activated glial cells,
represents one of the key factors in the pathogenesis of PD. The aim of this study was
to assess non-motor symptoms of PD as well as the expression of gene markers of
microgliosis, astrogliosis and neuroinflammation in a transgenic A53T mouse model,
which is recommended for studying PD and other synucleinopathies. These transgenic
mice express an A53T missense mutant form of human alpha-synuclein under the
control of the murine prion promoter.
Depression-like symptoms and anhedonia were assessed using Tail Suspension Test
and Sucrose Preference Test respectively, while memory deficits were assessed using
Novel Object Recognition Test along with Object Relocation Test. Gene expression of
ionized calcium-binding adapter molecule (Iba-1), glial fibrillary acidic protein
(GFAP α and δ form) and cytokines associated with the microglial pro-inflammatory
phenotype (TNFα, IL-1β, IL-6) was assessed using RT-PCR. Brain regions of interest
were the prefrontal cortex, hippocampus, and striatum of 6 months old A53T male
mice. Non-transgenic littermates were used as controls. Obtained results suggest that
A53T mice compared to non-transgenic littermates exert 1) the presence of memory
deficits and anhedonia, 2) no difference in the expression of glial activity markers and
pro-inflammatory cytokines in the cortex and striatum, and 3) increased expression of
glial activity markers in the hippocampus, but without significant changes in the
expression of cytokines. Overall, better understanding of the role of active glia in the
model is needed.",
publisher = "Belgrade: Serbian Neuroscience Society",
journal = "Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia",
title = "Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease",
pages = "109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5830"
}

Dubljević, O., Pavković, Ž., Srbovan, M., Potrebić, M.,& Pešić, V.. (2023). Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia
Belgrade: Serbian Neuroscience Society., 109.
https://hdl.handle.net/21.15107/rcub_ibiss_5830

Dubljević O, Pavković Ž, Srbovan M, Potrebić M, Pešić V. Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease. in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia. 2023;:109.
https://hdl.handle.net/21.15107/rcub_ibiss_5830 .

Dubljević, Olga, Pavković, Željko, Srbovan, Maja, Potrebić, Milica, Pešić, Vesna, "Upregulation of glial markers with absence of a typical proinflammatory profile in the hippocampus of A53T mice as a model of Parkinson’s disease" in Book of abstracts: 8th Congress of Serbian neuroscience society with international participation; 2023 May 31 - Jun 2; Belgrade, Serbia (2023):109,
https://hdl.handle.net/21.15107/rcub_ibiss_5830 .

TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Srbovan, Maja
AU  - Đmura, Goran
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5336
AB  - Changes in housing density, including individual housing, are commonly necessary in animal research. Obtaining reproducibility and translational validity in biomedical research requires an understanding of how animals adapt to changes in housing density. Existing literature mainly addresses acclimatization after transportation. We used a within-subject design to examine changes in behavior and weight gain of 4-mo-old male Wistar Han rats after reduction of their social group (RSG; due to removal of one rat from a cage containing 3 rats) and social isolation (SI; the removed rat) for the subsequent 2 wk. Changes in weight gain and in exploratory and center-avoidance behavior in an inescapable open arena (OA) were measured before (D0) and on days 7 and 14 (D7 and D14, respectively) after social change. The motor response to d-amphetamine (1.5 mg/kg), which stimulates behavioral arousal in response to novelty, was assessed at D14. Within-subject design revealed that RSG rats in OA had less locomotion at D7 but not more center-avoidance behavior and had returned to the D0 activity level at D14; SI rats in OA had consistently less locomotion and more center-avoidance behavior. Rearing behavior during OA exposure did not change in either group. However, SI rats showed more center-avoidance behavior in OA, greater weight gain, and less amphetamine-induced rearing at D14 as compared with RSG rats. These data indicate that after RSG, mature adult male rats require 2 wk to return to their baseline level of OA-related behavior, while after SI they gain weight and acquire maladaptive exploratory and center-avoidance behavior. The finding that SI produces maladaptive behavioral and physiologic alterations in adult male rats deserves attention because these changes could have confounding effects on research findings.
PB  - Memphis: American Association for Laboratory Animal Science
T2  - Journal of the American Association for Laboratory Animal Science
T1  - Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation
IS  - 6
VL  - 61
DO  - 10.30802/AALAS-JAALAS-22-000032
SP  - 615
EP  - 623
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Srbovan, Maja and Đmura, Goran and Pešić, Vesna",
year = "2022",
abstract = "Changes in housing density, including individual housing, are commonly necessary in animal research. Obtaining reproducibility and translational validity in biomedical research requires an understanding of how animals adapt to changes in housing density. Existing literature mainly addresses acclimatization after transportation. We used a within-subject design to examine changes in behavior and weight gain of 4-mo-old male Wistar Han rats after reduction of their social group (RSG; due to removal of one rat from a cage containing 3 rats) and social isolation (SI; the removed rat) for the subsequent 2 wk. Changes in weight gain and in exploratory and center-avoidance behavior in an inescapable open arena (OA) were measured before (D0) and on days 7 and 14 (D7 and D14, respectively) after social change. The motor response to d-amphetamine (1.5 mg/kg), which stimulates behavioral arousal in response to novelty, was assessed at D14. Within-subject design revealed that RSG rats in OA had less locomotion at D7 but not more center-avoidance behavior and had returned to the D0 activity level at D14; SI rats in OA had consistently less locomotion and more center-avoidance behavior. Rearing behavior during OA exposure did not change in either group. However, SI rats showed more center-avoidance behavior in OA, greater weight gain, and less amphetamine-induced rearing at D14 as compared with RSG rats. These data indicate that after RSG, mature adult male rats require 2 wk to return to their baseline level of OA-related behavior, while after SI they gain weight and acquire maladaptive exploratory and center-avoidance behavior. The finding that SI produces maladaptive behavioral and physiologic alterations in adult male rats deserves attention because these changes could have confounding effects on research findings.",
publisher = "Memphis: American Association for Laboratory Animal Science",
journal = "Journal of the American Association for Laboratory Animal Science",
title = "Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation",
number = "6",
volume = "61",
doi = "10.30802/AALAS-JAALAS-22-000032",
pages = "615-623"
}

Potrebić, M., Pavković, Ž., Srbovan, M., Đmura, G.,& Pešić, V.. (2022). Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation. in Journal of the American Association for Laboratory Animal Science
Memphis: American Association for Laboratory Animal Science., 61(6), 615-623.
https://doi.org/10.30802/AALAS-JAALAS-22-000032

Potrebić M, Pavković Ž, Srbovan M, Đmura G, Pešić V. Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation. in Journal of the American Association for Laboratory Animal Science. 2022;61(6):615-623.
doi:10.30802/AALAS-JAALAS-22-000032 .

Potrebić, Milica, Pavković, Željko, Srbovan, Maja, Đmura, Goran, Pešić, Vesna, "Changes in the Behavior and Body Weight of Mature, Adult Male Wistar Han Rats after Reduced Social Grouping and Social Isolation" in Journal of the American Association for Laboratory Animal Science, 61, no. 6 (2022):615-623,
https://doi.org/10.30802/AALAS-JAALAS-22-000032 . .

TY  - JOUR
AU  - Dubljević, Olga
AU  - Ković, Vanja
AU  - Pavković, Željko
AU  - Mitić, Miloš
AU  - Pešić, Vesna
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9599889
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5178
AB  - A potential relationship between unrestricted sucrose intake (USI), overweight, and emotional/behavioral control has not been well documented. We examined the influence of USI and having less sweetness than expected on body weight (BW), motor/exploratory, anxiety-like, and social dominant behavior in adult C57BL/6J male mice. Animals had free access to water (group 1) or 32% sucrose and water (sucrose groups 2-5) for 10 days. Then, group 2 remained with 32% sucrose while groups 3-5 were subjected to the downshift (24 h access to 4%, 8%, or 16% sucrose). All experimental groups were weighed and tested in the novel-open arena (NA), elevated plus maze (EPM), and tube tests to assess BW, motor/exploratory, anxiety-like, and social dominance behavior, respectively. USI did not influence animals' BW but produced hyperactivity and anxiolytic-like behavior, which was evident in EPM but not in NA; the outcomes of the downshift were comparable. USI did not influence successes/wins in the tube test but altered emotions that drive the winning, favoring a less anxious behavioral phenotype; this was not evident in the downshifted groups. Observed findings suggest that USI promotes sensation-seeking and motivates dominance, without changing BW, while blunted emotional base of social dominance might be an early mark of the downshift.
PB  - Basel: MDPI
T2  - Brain Sciences
T1  - The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model
IS  - 10
VL  - 12
DO  - 10.3390/brainsci12101332
SP  - 1332
ER  - 

@article{
author = "Dubljević, Olga and Ković, Vanja and Pavković, Željko and Mitić, Miloš and Pešić, Vesna",
year = "2022",
abstract = "A potential relationship between unrestricted sucrose intake (USI), overweight, and emotional/behavioral control has not been well documented. We examined the influence of USI and having less sweetness than expected on body weight (BW), motor/exploratory, anxiety-like, and social dominant behavior in adult C57BL/6J male mice. Animals had free access to water (group 1) or 32% sucrose and water (sucrose groups 2-5) for 10 days. Then, group 2 remained with 32% sucrose while groups 3-5 were subjected to the downshift (24 h access to 4%, 8%, or 16% sucrose). All experimental groups were weighed and tested in the novel-open arena (NA), elevated plus maze (EPM), and tube tests to assess BW, motor/exploratory, anxiety-like, and social dominance behavior, respectively. USI did not influence animals' BW but produced hyperactivity and anxiolytic-like behavior, which was evident in EPM but not in NA; the outcomes of the downshift were comparable. USI did not influence successes/wins in the tube test but altered emotions that drive the winning, favoring a less anxious behavioral phenotype; this was not evident in the downshifted groups. Observed findings suggest that USI promotes sensation-seeking and motivates dominance, without changing BW, while blunted emotional base of social dominance might be an early mark of the downshift.",
publisher = "Basel: MDPI",
journal = "Brain Sciences",
title = "The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model",
number = "10",
volume = "12",
doi = "10.3390/brainsci12101332",
pages = "1332"
}

Dubljević, O., Ković, V., Pavković, Ž., Mitić, M.,& Pešić, V.. (2022). The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model. in Brain Sciences
Basel: MDPI., 12(10), 1332.
https://doi.org/10.3390/brainsci12101332

Dubljević O, Ković V, Pavković Ž, Mitić M, Pešić V. The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model. in Brain Sciences. 2022;12(10):1332.
doi:10.3390/brainsci12101332 .

Dubljević, Olga, Ković, Vanja, Pavković, Željko, Mitić, Miloš, Pešić, Vesna, "The Influence of Unlimited Sucrose Intake on Body Weight and Behavior-Findings from a Mouse Model" in Brain Sciences, 12, no. 10 (2022):1332,
https://doi.org/10.3390/brainsci12101332 . .

TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Puškaš, Nela
AU  - Pešić, Vesna
PY  - 2022
UR  - https://www.frontiersin.org/articles/10.3389/fnbeh.2022.872628/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9113078
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4988
AB  - The fulfillment of belonging needs underlies a variety of behaviors. In order to understand how social needs unmet during maturation shape everyday life, we examined social motivation and cognition in peripubertal rats, as a rodent model of adolescence, subjected to social isolation (SI) during early and early-to-mid adolescence. The behavioral correlates of social orientation (social space preference), sociability (preference for social over non-social novelty), and social novelty preference (SNP) were examined in group-housed (GH) and single-housed (SH) rats in a 3-chamber test. The response to social odors was examined to gain insights into the developmental role of social odors in motivated social behavior. Differentiation between appetitive (number of visits/approaches) and consummatory (exploratory time) aspects of motivated social behavior was done to determine which facet of social motivation characterizes maturation when social needs are met and which aspect dominates when social needs are unsatisfied. The SI-sensitive parvalbumin-expressing interneurons (PVI) in the hippocampus were examined using immunohistochemistry. The main findings are the following: (1) in GH rats, the preference for social space is not evident regardless of animals' age, while sociability becomes apparent in mid-adolescence strictly through consummatory behavior, along with complete SNP (appetitive, consummatory); (2) SH promotes staying in a social chamber/space regardless of animals' age and produces an appetitive preference for it only in early-adolescent animals; (3) SH promotes sociability (appetitive, consummatory) regardless of the animals' age and prevents the SNP; (4) the preference for a social odor is displayed in all the groups through consummatory behavior, while appetitive behavior is evident only in SH rats; (5) the response to social odors does not commensurate directly to the response to conspecifics; (6) SH does not influence PVI in the hippocampus, except in the case of early-adolescence when a transient decrease in the dentate gyrus is observed. These results accentuate the developmental complexity of social motivation and cognition, and the power of SI in adolescence to infringe social maturation at different functional levels, promoting appetitive behavior toward peers overall but harming the interest for social novelty. The findings emphasize the importance of the fulfillment of basic social needs in the navigation through the social world.
PB  - Lausanne: Frontiers Media S.A.
T2  - Frontiers in Behavioral Neuroscience
T1  - The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.
VL  - 16
DO  - 10.3389/fnbeh.2022.872628
SP  - 872628
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Puškaš, Nela and Pešić, Vesna",
year = "2022",
abstract = "The fulfillment of belonging needs underlies a variety of behaviors. In order to understand how social needs unmet during maturation shape everyday life, we examined social motivation and cognition in peripubertal rats, as a rodent model of adolescence, subjected to social isolation (SI) during early and early-to-mid adolescence. The behavioral correlates of social orientation (social space preference), sociability (preference for social over non-social novelty), and social novelty preference (SNP) were examined in group-housed (GH) and single-housed (SH) rats in a 3-chamber test. The response to social odors was examined to gain insights into the developmental role of social odors in motivated social behavior. Differentiation between appetitive (number of visits/approaches) and consummatory (exploratory time) aspects of motivated social behavior was done to determine which facet of social motivation characterizes maturation when social needs are met and which aspect dominates when social needs are unsatisfied. The SI-sensitive parvalbumin-expressing interneurons (PVI) in the hippocampus were examined using immunohistochemistry. The main findings are the following: (1) in GH rats, the preference for social space is not evident regardless of animals' age, while sociability becomes apparent in mid-adolescence strictly through consummatory behavior, along with complete SNP (appetitive, consummatory); (2) SH promotes staying in a social chamber/space regardless of animals' age and produces an appetitive preference for it only in early-adolescent animals; (3) SH promotes sociability (appetitive, consummatory) regardless of the animals' age and prevents the SNP; (4) the preference for a social odor is displayed in all the groups through consummatory behavior, while appetitive behavior is evident only in SH rats; (5) the response to social odors does not commensurate directly to the response to conspecifics; (6) SH does not influence PVI in the hippocampus, except in the case of early-adolescence when a transient decrease in the dentate gyrus is observed. These results accentuate the developmental complexity of social motivation and cognition, and the power of SI in adolescence to infringe social maturation at different functional levels, promoting appetitive behavior toward peers overall but harming the interest for social novelty. The findings emphasize the importance of the fulfillment of basic social needs in the navigation through the social world.",
publisher = "Lausanne: Frontiers Media S.A.",
journal = "Frontiers in Behavioral Neuroscience",
title = "The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.",
volume = "16",
doi = "10.3389/fnbeh.2022.872628",
pages = "872628"
}

Potrebić, M., Pavković, Ž., Puškaš, N.,& Pešić, V.. (2022). The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.. in Frontiers in Behavioral Neuroscience
Lausanne: Frontiers Media S.A.., 16, 872628.
https://doi.org/10.3389/fnbeh.2022.872628

Potrebić M, Pavković Ž, Puškaš N, Pešić V. The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence.. in Frontiers in Behavioral Neuroscience. 2022;16:872628.
doi:10.3389/fnbeh.2022.872628 .

Potrebić, Milica, Pavković, Željko, Puškaš, Nela, Pešić, Vesna, "The Influence of Social Isolation on Social Orientation, Sociability, Social Novelty Preference, and Hippocampal Parvalbumin-Expressing Interneurons in Peripubertal Rats - Understanding the Importance of Meeting Social Needs in Adolescence." in Frontiers in Behavioral Neuroscience, 16 (2022):872628,
https://doi.org/10.3389/fnbeh.2022.872628 . .

TY  - CONF
AU  - Pavković, Željko
AU  - Potrebić, Milica
AU  - Dubljević, Olga
AU  - Srbovan, Maja
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5614
AB  - Познато је да су анксиозни поремећаји генерално изражени током адолесценције док специфичности везане за интензитет симптома током различитих фаза адолесценције (рана, средња, касна) још увек нису довољно проучене. Aдолесценција је као период матурације присутна код свих сисара, што оправдава коришћење анималних модела у психобиологији. Имајући у виду дефинисане фазе адолесценције глодара, испитивали смо матурацију експлораторног и анксиозног одговора на новину код мужјака Wistar Han пацова старих 35 ± 1 дан (крај ране/почетак средње адолесценције), 49 ± 1 дан (крај средње/почетак касне адолесценције) и 155 ± 1 дан (адултно доба). Коришћени су тестови у којима животиња слободно истражује нову средину (нова правоугаона арена, црно-бела кутија, уздигнути крстасти лавиринт). Адаптабилност на нову средину је процењивана поређењем бихејвиоралних одговора у првих и последњих 5 минута тестирања. Добијени резултати су показали да код пацова 1) матурациона крива експлораторног одговора на нову средину може имати како линеарни тако и U-профил, у зависности од карактеристика новe средине, 2) понашање налик анксиозном има инвертовани U-профил, док се 3) прилагодљивост независно од срединских услова јавља крајем средње/почетком касне адолесценције. Дискутован је потенцијални значај добијених резултата.
AB  - Poznato je da su anksiozni poremećaji generalno izraženi tokom adolescencije dok specifičnosti vezane za intenzitet simptoma tokom različitih faza adolescencije (rana, srednja, kasna) još uvek nisu dovoljno proučene. Adolescencija je kao period maturacije prisutna kod svih sisara, što opravdava korišćenje animalnih modela u psihobiologiji. Imajući u vidu definisane faze adolescencije glodara, ispitivali smo maturaciju eksploratornog i anksioznog odgovora na novinu kod mužjaka Wistar Han pacova starih 35 ± 1 dan (kraj rane/početak srednje adolescencije), 49 ± 1 dan (kraj srednje/početak kasne adolescencije) i 155 ± 1 dan (adultno doba). Korišćeni su testovi u kojima životinja slobodno istražuje novu sredinu (nova pravougaona arena, crno-bela kutija, uzdignuti krstasti lavirint). Adaptabilnost na novu sredinu je procenjivana poređenjem bihejvioralnih odgovora u prvih i poslednjih 5 minuta testiranja. Dobijeni rezultati su pokazali da kod pacova 1) maturaciona kriva eksploratornog odgovora na novu sredinu može imati kako linearni tako i U-profil, u zavisnosti od karakteristika nove sredine, 2) ponašanje nalik anksioznom ima invertovani U-profil, dok se 3) prilagodljivost nezavisno od sredinskih uslova javlja krajem srednje/početkom kasne adolescencije. Diskutovan je potencijalni značaj dobijenih rezultata.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова
T1  - Maturacija eksploratornog, anksioznog i adaptivnog odgovora na nove sredine u modelu pacova
SP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5614
ER  - 

@conference{
author = "Pavković, Željko and Potrebić, Milica and Dubljević, Olga and Srbovan, Maja and Pešić, Vesna",
year = "2022",
abstract = "Познато је да су анксиозни поремећаји генерално изражени током адолесценције док специфичности везане за интензитет симптома током различитих фаза адолесценције (рана, средња, касна) још увек нису довољно проучене. Aдолесценција је као период матурације присутна код свих сисара, што оправдава коришћење анималних модела у психобиологији. Имајући у виду дефинисане фазе адолесценције глодара, испитивали смо матурацију експлораторног и анксиозног одговора на новину код мужјака Wistar Han пацова старих 35 ± 1 дан (крај ране/почетак средње адолесценције), 49 ± 1 дан (крај средње/почетак касне адолесценције) и 155 ± 1 дан (адултно доба). Коришћени су тестови у којима животиња слободно истражује нову средину (нова правоугаона арена, црно-бела кутија, уздигнути крстасти лавиринт). Адаптабилност на нову средину је процењивана поређењем бихејвиоралних одговора у првих и последњих 5 минута тестирања. Добијени резултати су показали да код пацова 1) матурациона крива експлораторног одговора на нову средину може имати како линеарни тако и U-профил, у зависности од карактеристика новe средине, 2) понашање налик анксиозном има инвертовани U-профил, док се 3) прилагодљивост независно од срединских услова јавља крајем средње/почетком касне адолесценције. Дискутован је потенцијални значај добијених резултата., Poznato je da su anksiozni poremećaji generalno izraženi tokom adolescencije dok specifičnosti vezane za intenzitet simptoma tokom različitih faza adolescencije (rana, srednja, kasna) još uvek nisu dovoljno proučene. Adolescencija je kao period maturacije prisutna kod svih sisara, što opravdava korišćenje animalnih modela u psihobiologiji. Imajući u vidu definisane faze adolescencije glodara, ispitivali smo maturaciju eksploratornog i anksioznog odgovora na novinu kod mužjaka Wistar Han pacova starih 35 ± 1 dan (kraj rane/početak srednje adolescencije), 49 ± 1 dan (kraj srednje/početak kasne adolescencije) i 155 ± 1 dan (adultno doba). Korišćeni su testovi u kojima životinja slobodno istražuje novu sredinu (nova pravougaona arena, crno-bela kutija, uzdignuti krstasti lavirint). Adaptabilnost na novu sredinu je procenjivana poređenjem bihejvioralnih odgovora u prvih i poslednjih 5 minuta testiranja. Dobijeni rezultati su pokazali da kod pacova 1) maturaciona kriva eksploratornog odgovora na novu sredinu može imati kako linearni tako i U-profil, u zavisnosti od karakteristika nove sredine, 2) ponašanje nalik anksioznom ima invertovani U-profil, dok se 3) prilagodljivost nezavisno od sredinskih uslova javlja krajem srednje/početkom kasne adolescencije. Diskutovan je potencijalni značaj dobijenih rezultata.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова, Maturacija eksploratornog, anksioznog i adaptivnog odgovora na nove sredine u modelu pacova",
pages = "339",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5614"
}

Pavković, Ž., Potrebić, M., Dubljević, O., Srbovan, M.,& Pešić, V.. (2022). Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 339.
https://hdl.handle.net/21.15107/rcub_ibiss_5614

Pavković Ž, Potrebić M, Dubljević O, Srbovan M, Pešić V. Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:339.
https://hdl.handle.net/21.15107/rcub_ibiss_5614 .

Pavković, Željko, Potrebić, Milica, Dubljević, Olga, Srbovan, Maja, Pešić, Vesna, "Матурација експлораторног, анксиозног и адаптивног одговора на нове средине у моделу пацова" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):339,
https://hdl.handle.net/21.15107/rcub_ibiss_5614 .

TY  - CONF
AU  - Dubljević, Olga
AU  - Ković, Vanja
AU  - Pavković, Željko
AU  - Mitić, Miloš
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5617
AB  - Већина доступних животињских модела зависности од шећера не укључује компоненту само-администрирања супстанце, а познато је да се код људи конзумирање шећера одвија у ad libitum условима. Како би превазишли овај проблем, у овој студији испитани су конзуматорни и бихевиорални исходи неограниченог уноса шећера, као и накнадног смањења доступности шећера код адултних мужјака C57BL/6J мишева применом модификоване парадигме „Конзуматорни сукцесивни негативни контраст“ (cSNC).1,2 Две групе животиња имале су неограничен приступ 32% раствору сахарозе 10 дана (у виду тзв. Two- Bottle-Choice протокола); 11. дана једна група добила је раствор са мањом концентрацијом сахарозе (32%→4%), док је друга група добила уобичајени 32% раствор. Контролна група је имала ad libitum приступ води. Дванаестог дана стандардизованим тестовима понашања (Тест отвореног поља, Крстасти лавиринт и Туба тест социјалне конфронтације) испитани су социјална доминација, као и новином-изазвано експлораторно и понашање слично анксиозности. Добијени резултати указују на централни утицај неограниченог уноса шећера с обзиром да је код C57BL/6J мужјака уочен анксиолитички ефекат на понашање који вероватно одражава физиолошку дисрегулацију и склоност тражењу сензација. Потенцијални простор за примену нове модификоване парадигме у испитивању патолошких последица неограниченог уноса шећера даље је размотрен.
AB  - Većina dostupnih životinjskih modela zavisnosti od šećera ne uključuje komponentu samo-administriranja supstance, a poznato je da se kod ljudi konzumiranje šećera odvija u ad libitum uslovima. Kako bi prevazišli ovaj problem, u ovoj studiji ispitani su konzumatorni i bihevioralni ishodi neograničenog unosa šećera, kao i naknadnog smanjenja dostupnosti šećera kod adultnih mužjaka C57BL/6J miševa primenom modifikovane paradigme „Konzumatorni sukcesivni negativni kontrast“ (cSNC).1,2 Dve grupe životinja imale su neograničen pristup 32% rastvoru saharoze 10 dana (u vidu tzv. Two- Bottle-Choice protokola); 11. dana jedna grupa dobila je rastvor sa manjom koncentracijom saharoze (32%→4%), dok je druga grupa dobila uobičajeni 32% rastvor. Kontrolna grupa je imala ad libitum pristup vodi. Dvanaestog dana standardizovanim testovima ponašanja (Test otvorenog polja, Krstasti lavirint i Tuba test socijalne konfrontacije) ispitani su socijalna dominacija, kao i novinom-izazvano eksploratorno i ponašanje slično anksioznosti. Dobijeni rezultati ukazuju na centralni uticaj neograničenog unosa šećera s obzirom da je kod C57BL/6J mužjaka uočen anksiolitički efekat na ponašanje koji verovatno odražava fiziološku disregulaciju i sklonost traženju senzacija. Potencijalni prostor za primenu nove modifikovane paradigme u ispitivanju patoloških posledica neograničenog unosa šećera dalje je razmotren.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера
T1  - Konzumatorne i bihevioralne posledice neograničenog unosa šećera i naknadnog smanjenja dostupnosti šećera
SP  - 384
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5617
ER  - 

@conference{
author = "Dubljević, Olga and Ković, Vanja and Pavković, Željko and Mitić, Miloš and Pešić, Vesna",
year = "2022",
abstract = "Већина доступних животињских модела зависности од шећера не укључује компоненту само-администрирања супстанце, а познато је да се код људи конзумирање шећера одвија у ad libitum условима. Како би превазишли овај проблем, у овој студији испитани су конзуматорни и бихевиорални исходи неограниченог уноса шећера, као и накнадног смањења доступности шећера код адултних мужјака C57BL/6J мишева применом модификоване парадигме „Конзуматорни сукцесивни негативни контраст“ (cSNC).1,2 Две групе животиња имале су неограничен приступ 32% раствору сахарозе 10 дана (у виду тзв. Two- Bottle-Choice протокола); 11. дана једна група добила је раствор са мањом концентрацијом сахарозе (32%→4%), док је друга група добила уобичајени 32% раствор. Контролна група је имала ad libitum приступ води. Дванаестог дана стандардизованим тестовима понашања (Тест отвореног поља, Крстасти лавиринт и Туба тест социјалне конфронтације) испитани су социјална доминација, као и новином-изазвано експлораторно и понашање слично анксиозности. Добијени резултати указују на централни утицај неограниченог уноса шећера с обзиром да је код C57BL/6J мужјака уочен анксиолитички ефекат на понашање који вероватно одражава физиолошку дисрегулацију и склоност тражењу сензација. Потенцијални простор за примену нове модификоване парадигме у испитивању патолошких последица неограниченог уноса шећера даље је размотрен., Većina dostupnih životinjskih modela zavisnosti od šećera ne uključuje komponentu samo-administriranja supstance, a poznato je da se kod ljudi konzumiranje šećera odvija u ad libitum uslovima. Kako bi prevazišli ovaj problem, u ovoj studiji ispitani su konzumatorni i bihevioralni ishodi neograničenog unosa šećera, kao i naknadnog smanjenja dostupnosti šećera kod adultnih mužjaka C57BL/6J miševa primenom modifikovane paradigme „Konzumatorni sukcesivni negativni kontrast“ (cSNC).1,2 Dve grupe životinja imale su neograničen pristup 32% rastvoru saharoze 10 dana (u vidu tzv. Two- Bottle-Choice protokola); 11. dana jedna grupa dobila je rastvor sa manjom koncentracijom saharoze (32%→4%), dok je druga grupa dobila uobičajeni 32% rastvor. Kontrolna grupa je imala ad libitum pristup vodi. Dvanaestog dana standardizovanim testovima ponašanja (Test otvorenog polja, Krstasti lavirint i Tuba test socijalne konfrontacije) ispitani su socijalna dominacija, kao i novinom-izazvano eksploratorno i ponašanje slično anksioznosti. Dobijeni rezultati ukazuju na centralni uticaj neograničenog unosa šećera s obzirom da je kod C57BL/6J mužjaka uočen anksiolitički efekat na ponašanje koji verovatno odražava fiziološku disregulaciju i sklonost traženju senzacija. Potencijalni prostor za primenu nove modifikovane paradigme u ispitivanju patoloških posledica neograničenog unosa šećera dalje je razmotren.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера, Konzumatorne i bihevioralne posledice neograničenog unosa šećera i naknadnog smanjenja dostupnosti šećera",
pages = "384",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5617"
}

Dubljević, O., Ković, V., Pavković, Ž., Mitić, M.,& Pešić, V.. (2022). Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 384.
https://hdl.handle.net/21.15107/rcub_ibiss_5617

Dubljević O, Ković V, Pavković Ž, Mitić M, Pešić V. Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:384.
https://hdl.handle.net/21.15107/rcub_ibiss_5617 .

Dubljević, Olga, Ković, Vanja, Pavković, Željko, Mitić, Miloš, Pešić, Vesna, "Конзуматорне и бихевиоралне последице неограниченог уноса шећера и  накнадног смањења доступности шећера" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):384,
https://hdl.handle.net/21.15107/rcub_ibiss_5617 .

TY  - CONF
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Pešić, Vesna
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5609
AB  - The influence of parental social experience on the offspring neurodevelopment and its own susceptibility to the sculpting effect of social experience are not well understood. Using rat model we aimed to examine whether and how social experience of adolescent parents impacts psychophysical characteristics of their offspring, during pre-weaning period and later during adolescent growing in defined social conditions. 
Methods: Peripubertal Wistar rats (both sexes) were randomly selected for group housing (GH; n=3 per cage) or single housing (SH; n=1 per cage) at postnatal day (P) 29 and left undisturbed until P55. Thereafter, they were grouped for mating (SH males with SH females, GH males with GH females). Body weight (BW) of pups was monitored at P7, P14, and P21. At P17 motor activity of the offspring was monitored; at P29 the animals were subjected to GH or SH; after 1 week (P36) and 2 weeks (P43) their motor activity was monitored; at P45 they were subjected to sucrose preference test (SPT). 
Results: The male offspring of SH parents had decreased locomotor activity in a novel open arena, at P17. At P36 and P43, adolescent SH offspring showed hypolocomotion in a novel arena and hypohedonic behavior in SPT compared to GH counterparts, regardless of parental experience. BW did not differ between groups. 
Conclusions: Parental social experience influences novelty-exploring behavior of offspring, producing response below expected at P17. With further offspring development, their individual social experience has stronger impact on the appearance of depression-like phenotypes than parental social experience.
PB  - Federation of European Neuroscience Societies
C3  - E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
T1  - The influence of parental social experience of offspring novelty-exploring and drepression-like behavior
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5609
ER  - 

@conference{
author = "Potrebić, Milica and Pavković, Željko and Pešić, Vesna",
year = "2022",
abstract = "The influence of parental social experience on the offspring neurodevelopment and its own susceptibility to the sculpting effect of social experience are not well understood. Using rat model we aimed to examine whether and how social experience of adolescent parents impacts psychophysical characteristics of their offspring, during pre-weaning period and later during adolescent growing in defined social conditions. 
Methods: Peripubertal Wistar rats (both sexes) were randomly selected for group housing (GH; n=3 per cage) or single housing (SH; n=1 per cage) at postnatal day (P) 29 and left undisturbed until P55. Thereafter, they were grouped for mating (SH males with SH females, GH males with GH females). Body weight (BW) of pups was monitored at P7, P14, and P21. At P17 motor activity of the offspring was monitored; at P29 the animals were subjected to GH or SH; after 1 week (P36) and 2 weeks (P43) their motor activity was monitored; at P45 they were subjected to sucrose preference test (SPT). 
Results: The male offspring of SH parents had decreased locomotor activity in a novel open arena, at P17. At P36 and P43, adolescent SH offspring showed hypolocomotion in a novel arena and hypohedonic behavior in SPT compared to GH counterparts, regardless of parental experience. BW did not differ between groups. 
Conclusions: Parental social experience influences novelty-exploring behavior of offspring, producing response below expected at P17. With further offspring development, their individual social experience has stronger impact on the appearance of depression-like phenotypes than parental social experience.",
publisher = "Federation of European Neuroscience Societies",
journal = "E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France",
title = "The influence of parental social experience of offspring novelty-exploring and drepression-like behavior",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5609"
}

Potrebić, M., Pavković, Ž.,& Pešić, V.. (2022). The influence of parental social experience of offspring novelty-exploring and drepression-like behavior. in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France
Federation of European Neuroscience Societies..
https://hdl.handle.net/21.15107/rcub_ibiss_5609

Potrebić M, Pavković Ž, Pešić V. The influence of parental social experience of offspring novelty-exploring and drepression-like behavior. in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5609 .

Potrebić, Milica, Pavković, Željko, Pešić, Vesna, "The influence of parental social experience of offspring novelty-exploring and drepression-like behavior" in E-book of Abstracts: FENS Forum 2022; 2022 Jul 9-13; Paris, France (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5609 .

TY  - JOUR
AU  - Potrebić, Milica
AU  - Pavković, Željko
AU  - Lončarević-Vasiljković, Nataša
AU  - Kanazir, Selma
AU  - Pešić, Vesna
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4073
AB  - Reduction in direct social contact with peers during early adolescence is thought to be a risk factor for an increase in depressive symptoms, but there is still no clear evidence to suggest early behavioral manifestations and their association with the later outcome of social distancing during this period. To address this question, we used social isolation paradigm in peripubertal rats as the rodent model of adolescence. The litter was an experimental unit. On postnatal day 29, each litter gave group-housed and single-housed males, which were reared and tested one week and two weeks thereafter. Psychomotor/emotional response to novelty in exploration-based tasks, behavioral and neuronal responses to the drug reward (D-amphetamine), motivation/hedonic behavior, physiological and response to physiological stress were examined. Social isolation in peripubertal rats manifested through: hyper-reactivity/agitation and the state anxiety/risk-taking at an early stage; reduced behavioral response to D-amphetamine and altered neural processing of this stimulus, at a later stage; consummatory hypohedonia that deepened over time without changing the motivation to eat; unchanged body weight gain and resting blood corticosterone, cortisol and glucose levels over time; altered blood biochemistry (silenced corticosterone and increased glucose) due to overnight fasting only at an early stage. Our results highlight that the outcome of reduced direct social contact with peers during peripuberty is dynamic, with the cluster of atypical early symptoms that evolve into the syndrome that is delicate for assessment through routinely measurable behavior and biomarkers of stress, but with progressive consummatory hypohedonia and unaffected motivation to eat as stable marks.
PB  - Elsevier Inc.
T2  - Progress in Neuro-Psychopharmacology and Biological Psychiatry
T1  - Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty
VL  - 108
DO  - 10.1016/j.pnpbp.2020.110186
SP  - 110186
ER  - 

@article{
author = "Potrebić, Milica and Pavković, Željko and Lončarević-Vasiljković, Nataša and Kanazir, Selma and Pešić, Vesna",
year = "2021",
abstract = "Reduction in direct social contact with peers during early adolescence is thought to be a risk factor for an increase in depressive symptoms, but there is still no clear evidence to suggest early behavioral manifestations and their association with the later outcome of social distancing during this period. To address this question, we used social isolation paradigm in peripubertal rats as the rodent model of adolescence. The litter was an experimental unit. On postnatal day 29, each litter gave group-housed and single-housed males, which were reared and tested one week and two weeks thereafter. Psychomotor/emotional response to novelty in exploration-based tasks, behavioral and neuronal responses to the drug reward (D-amphetamine), motivation/hedonic behavior, physiological and response to physiological stress were examined. Social isolation in peripubertal rats manifested through: hyper-reactivity/agitation and the state anxiety/risk-taking at an early stage; reduced behavioral response to D-amphetamine and altered neural processing of this stimulus, at a later stage; consummatory hypohedonia that deepened over time without changing the motivation to eat; unchanged body weight gain and resting blood corticosterone, cortisol and glucose levels over time; altered blood biochemistry (silenced corticosterone and increased glucose) due to overnight fasting only at an early stage. Our results highlight that the outcome of reduced direct social contact with peers during peripuberty is dynamic, with the cluster of atypical early symptoms that evolve into the syndrome that is delicate for assessment through routinely measurable behavior and biomarkers of stress, but with progressive consummatory hypohedonia and unaffected motivation to eat as stable marks.",
publisher = "Elsevier Inc.",
journal = "Progress in Neuro-Psychopharmacology and Biological Psychiatry",
title = "Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty",
volume = "108",
doi = "10.1016/j.pnpbp.2020.110186",
pages = "110186"
}

Potrebić, M., Pavković, Ž., Lončarević-Vasiljković, N., Kanazir, S.,& Pešić, V.. (2021). Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty. in Progress in Neuro-Psychopharmacology and Biological Psychiatry
Elsevier Inc.., 108, 110186.
https://doi.org/10.1016/j.pnpbp.2020.110186

Potrebić M, Pavković Ž, Lončarević-Vasiljković N, Kanazir S, Pešić V. Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty. in Progress in Neuro-Psychopharmacology and Biological Psychiatry. 2021;108:110186.
doi:10.1016/j.pnpbp.2020.110186 .

Potrebić, Milica, Pavković, Željko, Lončarević-Vasiljković, Nataša, Kanazir, Selma, Pešić, Vesna, "Altered hedonic, novelty-, stress- and D-amphetamine-induced response to due to social isolation in peripuberty" in Progress in Neuro-Psychopharmacology and Biological Psychiatry, 108 (2021):110186,
https://doi.org/10.1016/j.pnpbp.2020.110186 . .

TY  - JOUR
AU  - Petković, Branka
AU  - Kesić, Srđan
AU  - Ristić, Slavica
AU  - Pavković, Željko
AU  - Podgorac, Jelena
AU  - Stojadinović, Gordana
AU  - Pešić, Vesna
PY  - 2020
UR  - https://www.eurekaselect.com/180519/article
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32213154
UR  - https://radar.ibiss.bg.ac.rs/123456789/3879
AB  - BACKGROUND Psychotic states related to psychostimulant misuse in patients with hepatitis C virus infection may complicate acceptance and reaction to antiviral treatment. This observation equally applies to widely used ribavirin therapy. OBJECTIVE We examined psychomotor and body weight gain response to low ribavirin doses after cessation of intermittent amphetamine treatment in adult rats to assess its role in neurobehavioral outcome during psychostimulant withdrawal. METHOD The model of amphetamine-induced (1.5 mg/kg/day, i.p., 7 consecutive days) motor sensitization and affected body weight gain was established in adult male Wistar rats. Then, additional cohort of amphetamine-sensitized rats was subjected to saline (0.9% NaCl; 1 mL/kg/day; i.p.) or ribavirin (10, 20 and 30 mg/kg/day, i.p.) treatment for 7 consecutive days. Animals' motor activity in a novel environment was monitored after the 1st and the 7th saline/ribavirin injection. Body weight gain was calculated as appropriate. Determination and quantification of ribavirin in the brain tissue were performed too. RESULTS The 1st application of ribavirin to amphetamine-sensitized rats affected/decreased their novelty-induced motor activity only at a dose of 30 mg/kg. After the 7th application, ribavirin 30 mg/kg/day still decreased while 10 and 20 mg/kg/day increased novelty-induced motor activity. These behavioral effects coincided with the time required to reach maximum ribavirin concentration in the brain. Body weight gain during withdrawal was not influenced by any of the doses tested. CONCLUSION Ribavirin displays central effects that in repeated treatment, depending on the applied dose, could significantly influence psychomotor response but not body weight gain during psychostimulant/amphetamine withdrawal.
PB  - Bentham Science Publishers Ltd.
T2  - Current Pharmaceutical Design
T1  - A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats
IS  - 31
VL  - 26
DO  - 10.2174/1381612826666200326125821
SP  - 3884
EP  - 3894
ER  - 

@article{
author = "Petković, Branka and Kesić, Srđan and Ristić, Slavica and Pavković, Željko and Podgorac, Jelena and Stojadinović, Gordana and Pešić, Vesna",
year = "2020",
abstract = "BACKGROUND Psychotic states related to psychostimulant misuse in patients with hepatitis C virus infection may complicate acceptance and reaction to antiviral treatment. This observation equally applies to widely used ribavirin therapy. OBJECTIVE We examined psychomotor and body weight gain response to low ribavirin doses after cessation of intermittent amphetamine treatment in adult rats to assess its role in neurobehavioral outcome during psychostimulant withdrawal. METHOD The model of amphetamine-induced (1.5 mg/kg/day, i.p., 7 consecutive days) motor sensitization and affected body weight gain was established in adult male Wistar rats. Then, additional cohort of amphetamine-sensitized rats was subjected to saline (0.9% NaCl; 1 mL/kg/day; i.p.) or ribavirin (10, 20 and 30 mg/kg/day, i.p.) treatment for 7 consecutive days. Animals' motor activity in a novel environment was monitored after the 1st and the 7th saline/ribavirin injection. Body weight gain was calculated as appropriate. Determination and quantification of ribavirin in the brain tissue were performed too. RESULTS The 1st application of ribavirin to amphetamine-sensitized rats affected/decreased their novelty-induced motor activity only at a dose of 30 mg/kg. After the 7th application, ribavirin 30 mg/kg/day still decreased while 10 and 20 mg/kg/day increased novelty-induced motor activity. These behavioral effects coincided with the time required to reach maximum ribavirin concentration in the brain. Body weight gain during withdrawal was not influenced by any of the doses tested. CONCLUSION Ribavirin displays central effects that in repeated treatment, depending on the applied dose, could significantly influence psychomotor response but not body weight gain during psychostimulant/amphetamine withdrawal.",
publisher = "Bentham Science Publishers Ltd.",
journal = "Current Pharmaceutical Design",
title = "A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats",
number = "31",
volume = "26",
doi = "10.2174/1381612826666200326125821",
pages = "3884-3894"
}

Petković, B., Kesić, S., Ristić, S., Pavković, Ž., Podgorac, J., Stojadinović, G.,& Pešić, V.. (2020). A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats. in Current Pharmaceutical Design
Bentham Science Publishers Ltd.., 26(31), 3884-3894.
https://doi.org/10.2174/1381612826666200326125821

Petković B, Kesić S, Ristić S, Pavković Ž, Podgorac J, Stojadinović G, Pešić V. A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats. in Current Pharmaceutical Design. 2020;26(31):3884-3894.
doi:10.2174/1381612826666200326125821 .

Petković, Branka, Kesić, Srđan, Ristić, Slavica, Pavković, Željko, Podgorac, Jelena, Stojadinović, Gordana, Pešić, Vesna, "A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats" in Current Pharmaceutical Design, 26, no. 31 (2020):3884-3894,
https://doi.org/10.2174/1381612826666200326125821 . .

TY  - JOUR
AU  - Petković, Branka
AU  - Kesić, Srđan
AU  - Ristić, Slavica
AU  - Pavković, Željko
AU  - Podgorac, Jelena
AU  - Stojadinović, Gordana
AU  - Pešić, Vesna
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3765
AB  - Background: Psychotic states related to psychostimulant misuse in patients with hepatitis C virus infection may complicate acceptance and reaction to antiviral treatment. This observation equally applies to widely used ribavirin therapy.
Objective: We examined psychomotor and body weight gain response to low ribavirin doses after cessation of intermittent amphetamine treatment in adult rats to assess its role in neurobehavioral outcome during psychostimulant withdrawal.
Method: The model of amphetamine-induced (1.5 mg/kg/day, i.p., 7 consecutive days) motor sensitization and affected body weight gain was established in adult male Wistar rats. Then, additional cohort of amphetamine-sensitized rats was subjected to saline (0.9% NaCl; 1 mL/kg/day; i.p.) or ribavirin (10, 20 and 30 mg/kg/day, i.p.) treatment for 7 consecutive days. Animals’ motor activity in a novel environment was monitored after the 1st and the 7th saline/ribavirin injection. Body weight gain was calculated as appropriate. Determination and quantification of ribavirin in the brain tissue were performed too.
Results: The 1st application of ribavirin to amphetamine-sensitized rats affected/decreased their novelty-induced motor activity only at a dose of 30 mg/kg. After the 7th application, ribavirin 30 mg/kg/day still decreased while 10 and 20 mg/kg/day increased novelty-induced motor activity. These behavioral effects coincided with the time required to reach maximum ribavirin concentration in the brain. Body weight gain during withdrawal was not influenced by any of the doses tested.
Conclusion: Ribavirin displays central effects that in repeated treatment, depending on the applied dose, could significantly influence psychomotor response but not body weight gain during psychostimulant/amphetamine withdrawal.
PB  - Bentham Science Publishers Ltd.
T2  - Current Pharmaceutical Design
T1  - A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats
DO  - 10.2174/1381612826666200326125821
ER  - 

@article{
author = "Petković, Branka and Kesić, Srđan and Ristić, Slavica and Pavković, Željko and Podgorac, Jelena and Stojadinović, Gordana and Pešić, Vesna",
year = "2020",
abstract = "Background: Psychotic states related to psychostimulant misuse in patients with hepatitis C virus infection may complicate acceptance and reaction to antiviral treatment. This observation equally applies to widely used ribavirin therapy.
Objective: We examined psychomotor and body weight gain response to low ribavirin doses after cessation of intermittent amphetamine treatment in adult rats to assess its role in neurobehavioral outcome during psychostimulant withdrawal.
Method: The model of amphetamine-induced (1.5 mg/kg/day, i.p., 7 consecutive days) motor sensitization and affected body weight gain was established in adult male Wistar rats. Then, additional cohort of amphetamine-sensitized rats was subjected to saline (0.9% NaCl; 1 mL/kg/day; i.p.) or ribavirin (10, 20 and 30 mg/kg/day, i.p.) treatment for 7 consecutive days. Animals’ motor activity in a novel environment was monitored after the 1st and the 7th saline/ribavirin injection. Body weight gain was calculated as appropriate. Determination and quantification of ribavirin in the brain tissue were performed too.
Results: The 1st application of ribavirin to amphetamine-sensitized rats affected/decreased their novelty-induced motor activity only at a dose of 30 mg/kg. After the 7th application, ribavirin 30 mg/kg/day still decreased while 10 and 20 mg/kg/day increased novelty-induced motor activity. These behavioral effects coincided with the time required to reach maximum ribavirin concentration in the brain. Body weight gain during withdrawal was not influenced by any of the doses tested.
Conclusion: Ribavirin displays central effects that in repeated treatment, depending on the applied dose, could significantly influence psychomotor response but not body weight gain during psychostimulant/amphetamine withdrawal.",
publisher = "Bentham Science Publishers Ltd.",
journal = "Current Pharmaceutical Design",
title = "A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats",
doi = "10.2174/1381612826666200326125821"
}

Petković, B., Kesić, S., Ristić, S., Pavković, Ž., Podgorac, J., Stojadinović, G.,& Pešić, V.. (2020). A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats. in Current Pharmaceutical Design
Bentham Science Publishers Ltd...
https://doi.org/10.2174/1381612826666200326125821

Petković B, Kesić S, Ristić S, Pavković Ž, Podgorac J, Stojadinović G, Pešić V. A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats. in Current Pharmaceutical Design. 2020;.
doi:10.2174/1381612826666200326125821 .

Petković, Branka, Kesić, Srđan, Ristić, Slavica, Pavković, Željko, Podgorac, Jelena, Stojadinović, Gordana, Pešić, Vesna, "A New Look at an Old Drug: Cumulative Effects of Low Ribavirin Doses in Amphetamine-Sensitized Rats" in Current Pharmaceutical Design (2020),
https://doi.org/10.2174/1381612826666200326125821 . .

TY  - JOUR
AU  - Pavković, Željko
AU  - Potrebić, Milica
AU  - Kanazir, Selma
AU  - Pešić, Vesna
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0278584619304245?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3455
AB  - Adolescent neurodevelopment confer vulnerability to the actions of treatments that produce adaptations in neurocircuitry underlying motivation, impulsivity and reward. Considering wide usage of a sedative-hypnotic agent propofol in clinical practice, we examined whether propofol is a challenging treatment for peripubertal brain. Motivation/hedonic behavior (sucrose preference test), approach/avoidance behavior (elevated plus maze test) and response to dissociative drug phencyclidine (PCP) were studied in peripubertal rats (the rodent model of periadolescence) after propofol anesthesia exposure (PAE). Neurodegeneration (Fluoro-Jade staining) and the expression of proteins (Western blot) involved in excitatory synaptic transmission and activity-dependent synaptic stabilization in the medial prefrontal cortex (mPFC) and striatum (components of motivation/reward circuitry; process both appetitive and aversive events) were examined as well. In peripubertal rats PAE produced 1) transient brain-region specific changes in the expression of N-methyl-d-aspartate (NMDA) receptor subunits NR2A and NR2B, PSD-95 and N-cadherin, without neurotoxicity, 2) hyperlocomotor response to PCP, 3) no changes in preference for palatable 1% sucrose solution and a decrease in food eaten, 4) preference for 20% sucrose solution without changes in food eaten, 5) stretch-attended postures and open arms entries in the elevated plus maze test. Overall, these novel findings show that PAE leaves transient synaptic trace recognized as early form of synaptic plasticity related to passive drug exposure in the brain systems implicated in motivation/reward, increases drug-responsiveness, favors risk-taking and preference of novel/intense stimuli repairing otherwise present motivational deficiency. These findings accentuate multifaceted response to propofol in peripuberty and the importance of environmental stability for the most favorable neurobehavioral recovery.
T2  - Progress in Neuro-Psychopharmacology and Biological Psychiatry
T1  - Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats.
VL  - 96
DO  - 10.1016/j.pnpbp.2019.109733
SP  - 109733
ER  - 

@article{
author = "Pavković, Željko and Potrebić, Milica and Kanazir, Selma and Pešić, Vesna",
year = "2019",
abstract = "Adolescent neurodevelopment confer vulnerability to the actions of treatments that produce adaptations in neurocircuitry underlying motivation, impulsivity and reward. Considering wide usage of a sedative-hypnotic agent propofol in clinical practice, we examined whether propofol is a challenging treatment for peripubertal brain. Motivation/hedonic behavior (sucrose preference test), approach/avoidance behavior (elevated plus maze test) and response to dissociative drug phencyclidine (PCP) were studied in peripubertal rats (the rodent model of periadolescence) after propofol anesthesia exposure (PAE). Neurodegeneration (Fluoro-Jade staining) and the expression of proteins (Western blot) involved in excitatory synaptic transmission and activity-dependent synaptic stabilization in the medial prefrontal cortex (mPFC) and striatum (components of motivation/reward circuitry; process both appetitive and aversive events) were examined as well. In peripubertal rats PAE produced 1) transient brain-region specific changes in the expression of N-methyl-d-aspartate (NMDA) receptor subunits NR2A and NR2B, PSD-95 and N-cadherin, without neurotoxicity, 2) hyperlocomotor response to PCP, 3) no changes in preference for palatable 1% sucrose solution and a decrease in food eaten, 4) preference for 20% sucrose solution without changes in food eaten, 5) stretch-attended postures and open arms entries in the elevated plus maze test. Overall, these novel findings show that PAE leaves transient synaptic trace recognized as early form of synaptic plasticity related to passive drug exposure in the brain systems implicated in motivation/reward, increases drug-responsiveness, favors risk-taking and preference of novel/intense stimuli repairing otherwise present motivational deficiency. These findings accentuate multifaceted response to propofol in peripuberty and the importance of environmental stability for the most favorable neurobehavioral recovery.",
journal = "Progress in Neuro-Psychopharmacology and Biological Psychiatry",
title = "Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats.",
volume = "96",
doi = "10.1016/j.pnpbp.2019.109733",
pages = "109733"
}

Pavković, Ž., Potrebić, M., Kanazir, S.,& Pešić, V.. (2019). Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats.. in Progress in Neuro-Psychopharmacology and Biological Psychiatry, 96, 109733.
https://doi.org/10.1016/j.pnpbp.2019.109733

Pavković Ž, Potrebić M, Kanazir S, Pešić V. Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats.. in Progress in Neuro-Psychopharmacology and Biological Psychiatry. 2019;96:109733.
doi:10.1016/j.pnpbp.2019.109733 .

Pavković, Željko, Potrebić, Milica, Kanazir, Selma, Pešić, Vesna, "Motivation, risk-taking and sensation seeking behavior in propofol anesthesia exposed peripubertal rats." in Progress in Neuro-Psychopharmacology and Biological Psychiatry, 96 (2019):109733,
https://doi.org/10.1016/j.pnpbp.2019.109733 . .

TY  - THES
AU  - Pavković, Željko
PY  - 2018
UR  - http://uvidok.rcub.bg.ac.rs/handle/123456789/2787
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3137
AB  - Propofol je često korišćeni anestetik u modernoj medicini. Adiktivni potencijal propofola je uočen, kao i uticaj na memorijski proces. Međutim, oba fenomena su još uvek nedovoljno istražena. Imajući u vidu da je adolescencija period izuzetne osetljivosti na dejstvo adiktivnih supstanci i intenzivne maturacije mnemoničkog potencijala, cilj ovog rada je bio da se ispita uticaj jednokratnog izlaganja propofolskoj anesteziji, što je tipičan način kliničke primene anestetika, na dopaminsku signalizaciju, sinaptičku i neuronsku aktivnost u različitim regionima mozga i ponašanje juvenilnih/peripubertetskih pacova, kao model sistema humanog periadolescentnog razvoja. Efekti su analizirani 4, 24 i 48 sati nakon tretamana, kod mužjaka Wistar pacova starih 35 dana. Dobijeni rezultati su po prvi put ukazali da izlaganje propofolskoj anesteziji izaziva promene u ekspresiji/fosforilaciji signalnih molekula koji su već prepoznati kao značajni za dejstvo adiktivnih supstanci. Od svih analiziranih dopaminoceptivnih moždanih regiona (medijalni prefrontalni korteks, strijatum i talamus) jedino su u talamusu uočene značajne promene u ekspresiji fosforilisane/aktivirane forme DARPP-32 proteina, pokazatelja postsinaptičke dopaminske signalizacije, 4 i 24 sata nakon tretmana, i bile su praćene povećanom ekspresijom FosB/ΔFosB proteina, biohemijskog pokazatelja neuronske aktivnosti. Promene su lokalizovane u paraventrikularnom talamičkom jedru i mediodorzalnom talamusu. U strijatumu i medijalnom prefrontalnom korteksu je uočen porast u ekspresiji fosforilisane forme CaMKIIα, biohemijskog senzora sinaptičke aktivnosti koji ima važnu ulogu u pamćenju prethodnog izlaganja adiktivnim supstancama. Smanjenje u intenzitetu anksioznosti (procenjeno na osnovu rezultata dobijenih u testu svetlo/tamne kutije i uzdignutog krstastog lavirinta) je zabeleženo 24 sata nakon tretmana, kada je uočen i pad u ekspresiji FosB proteina u strijatumu, što se može tumačiti kao traženje senzacija usled smanjene aktivnosti moždanog regiona značajnog za osećaj zadovoljstva i motivisanost. Pojačan motorički odgovor na d-amfetamin i fenciklidin je uočen 24 sata nakon tretmana (ukrštena senzitizacija), kao potvrda da bez obzira na različite primarne mehanizme dejstva propofol i dve korišćene droge koriste iste neuronske puteve za ostvarivanje psihomotoričkih efekata. Rezultati studije su takođe ukazali da kod peripubertetskih pacova nakon izlaganja propofolskoj anesteziji postoje izvesne poteškoće u pozivanju memorije i memorisanju novih informacija u neaverzivnim memorijskim testovima (prostorna habituacija, prepoznavanje novog objekta), 24 sata nakon tretmana. Ove posledice su bile praćene promenama u ekspresiji molekula koji učestvuju u pozivanju i rekonsolidaciji epizodične memorije (BDNF/TrkB, Egr-1, ERK1/2, CaMKIIα, FosB/ΔFosB), u dorzalnom hipokampusu. Uočene poteškoće u prepoznavanju novine su u skladu sa malobrojnim kliničkim studijama, koje naglašavaju značaj kontrole osnovnih kognitivnih funkcija kod starije dece nakon izlaganja propofolskoj anesteziji. Takođe, u svim ispitivanim moždanim regionima osim u talamusu je zabeležen porast u ekspresiji fosforilisane forme Aducinβ proteina, što je indikacija za destabilizaciju citoskeleta i sinaptičku reorganizaciju. Gledano u celini, rezultati ove studije su ukazali da bolje razumevanje bioloških posledica primene propofola u juvenilnom/pubertetskom uzrastu može doprineti sagledavanju starosno zavisnih zdravstvenih rizika tretmana, koji su do sada intenzivno istraživani samo u ekstremima životne dobi, tj. u ranoj postnatalnoj i kasnoj životnoj fazi.
AB  - Propofol is a commonly used anesthetic in modern medicine. Addictive potential of propofol is observed, as well as the impact on the memory process. However, both phenomena are still insufficiently explored. Bearing in mind that adolescence is a period of extreme sensitivity to addictive substances and intense maturation of the mnemonic potential, the aim of this study was to examine the effect of a single exposure to propofol anesthesia, which is a typical method of its clinical application, on dopaminergic signaling, synaptic and neuronal activity in different brain regions and behavior of juvenile/peripubertal rats, as a model system of human periadolescencent development. The effects were analyzed 4, 24 and 48 hours after the treatment, in male Wistar rats aged 35 days.
The obtained findings for the first time showed that exposure to propofol anesthesia caused changes in the expression/phosphorylation of signal molecules that are already recognized as significant for the action of the addictive substances. Of all the analyzed dopaminoceptive brain regions (medial prefrontal cortex, striatum and thalamus), significant changes in the expression of the phosphorylated/activated form of DARPP-32 protein, indicator of postsynaptic dopaminergic signalling, were observed only in the thalamus, 4 and 24 hours after the treatment, and were accompanied by increased expression of FosB/ΔFosB protein, a biochemical indicator of neuronal activity. The alterations were localized in the paraventricular thalamic nucleus and the mid-dorsal thalamus. An increase in the expression of the phosphorylated form of CaMKIIα, a biochemical sensor of synaptic activity that has an important role in memory on addictive substances exposure, was detected in striatum and medial prefrontal cortex. Reduction in the intensity of anxiety (estimated in accordence to the data obtained in the light/dark box and elevated plus maze tests) was observed 24 hours after the treatment, along with the decrease in the expression of FosB protein in striatum, which can be interpreted as a sensation seeking due to decreased activity of brain region important for a pleasure/motivation. An enhanced motor response to d-amphetamine and phencyclidine was observed 24 hours after the treatment (cross
sensitization), as confirmation that despite the different primary mechanisms of action, propofol and these two drugs use the same neuronal pathways to achieve psychomotor effects. The results of the study also indicated that in peripubertal rats exposed to propofol anesthesia, there were some difficulties in memory retrieval and acquisition of new learning in non-aversive memory tests (spatial habituation, novel object recognition), 24 hours after the treatment. These effects were accompanied by changes in the expression of molecules involved in retrieval and reconsolidation of episodic memory (BDNF/TrkB, Egr-1, ERK1/2, CaMKIIα, FosB/ΔFosB) in the dorsal hippocampus. Detected difficulties in recognizing the novelty are consistent with a few clinical studies that emphasize the importance of controlling basic cognitive functions in older children after exposure to propofol anesthesia. Also, in all investigated brain regions, except the thalamus, an increase in the expression of the phosphorylated form of Adducinβ protein was observed, which is an indication of the cytoskelet destabilization and synaptic reorganization.
Overall, the results of this study accentuated that a better understanding of the biological consequences of the use of propofol in juvenile/puberty period could contribute to the undestanding of age-dependent health risks of the treatment, which have been extensively investigated so far only at the extremes of age, i.e. during early postnatal period and in aging.
PB  - Belgrade: University of Belgrade, Faculty of Biology
T2  - University of Belgrade, Faculty of Biology
T1  - Efekti anestezije indukovane propofolom na sinaptičku plastičnost, aktivnost dopaminskog sistema i ponašanje juvenilnih pacova
T1  - The effects of propofol induced anesthesia on synaptic plasticity, dopaminergic system activity and behaviour of juvenile rats
SP  - 1
EP  - 156
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3137
ER  - 

@phdthesis{
author = "Pavković, Željko",
year = "2018",
abstract = "Propofol je često korišćeni anestetik u modernoj medicini. Adiktivni potencijal propofola je uočen, kao i uticaj na memorijski proces. Međutim, oba fenomena su još uvek nedovoljno istražena. Imajući u vidu da je adolescencija period izuzetne osetljivosti na dejstvo adiktivnih supstanci i intenzivne maturacije mnemoničkog potencijala, cilj ovog rada je bio da se ispita uticaj jednokratnog izlaganja propofolskoj anesteziji, što je tipičan način kliničke primene anestetika, na dopaminsku signalizaciju, sinaptičku i neuronsku aktivnost u različitim regionima mozga i ponašanje juvenilnih/peripubertetskih pacova, kao model sistema humanog periadolescentnog razvoja. Efekti su analizirani 4, 24 i 48 sati nakon tretamana, kod mužjaka Wistar pacova starih 35 dana. Dobijeni rezultati su po prvi put ukazali da izlaganje propofolskoj anesteziji izaziva promene u ekspresiji/fosforilaciji signalnih molekula koji su već prepoznati kao značajni za dejstvo adiktivnih supstanci. Od svih analiziranih dopaminoceptivnih moždanih regiona (medijalni prefrontalni korteks, strijatum i talamus) jedino su u talamusu uočene značajne promene u ekspresiji fosforilisane/aktivirane forme DARPP-32 proteina, pokazatelja postsinaptičke dopaminske signalizacije, 4 i 24 sata nakon tretmana, i bile su praćene povećanom ekspresijom FosB/ΔFosB proteina, biohemijskog pokazatelja neuronske aktivnosti. Promene su lokalizovane u paraventrikularnom talamičkom jedru i mediodorzalnom talamusu. U strijatumu i medijalnom prefrontalnom korteksu je uočen porast u ekspresiji fosforilisane forme CaMKIIα, biohemijskog senzora sinaptičke aktivnosti koji ima važnu ulogu u pamćenju prethodnog izlaganja adiktivnim supstancama. Smanjenje u intenzitetu anksioznosti (procenjeno na osnovu rezultata dobijenih u testu svetlo/tamne kutije i uzdignutog krstastog lavirinta) je zabeleženo 24 sata nakon tretmana, kada je uočen i pad u ekspresiji FosB proteina u strijatumu, što se može tumačiti kao traženje senzacija usled smanjene aktivnosti moždanog regiona značajnog za osećaj zadovoljstva i motivisanost. Pojačan motorički odgovor na d-amfetamin i fenciklidin je uočen 24 sata nakon tretmana (ukrštena senzitizacija), kao potvrda da bez obzira na različite primarne mehanizme dejstva propofol i dve korišćene droge koriste iste neuronske puteve za ostvarivanje psihomotoričkih efekata. Rezultati studije su takođe ukazali da kod peripubertetskih pacova nakon izlaganja propofolskoj anesteziji postoje izvesne poteškoće u pozivanju memorije i memorisanju novih informacija u neaverzivnim memorijskim testovima (prostorna habituacija, prepoznavanje novog objekta), 24 sata nakon tretmana. Ove posledice su bile praćene promenama u ekspresiji molekula koji učestvuju u pozivanju i rekonsolidaciji epizodične memorije (BDNF/TrkB, Egr-1, ERK1/2, CaMKIIα, FosB/ΔFosB), u dorzalnom hipokampusu. Uočene poteškoće u prepoznavanju novine su u skladu sa malobrojnim kliničkim studijama, koje naglašavaju značaj kontrole osnovnih kognitivnih funkcija kod starije dece nakon izlaganja propofolskoj anesteziji. Takođe, u svim ispitivanim moždanim regionima osim u talamusu je zabeležen porast u ekspresiji fosforilisane forme Aducinβ proteina, što je indikacija za destabilizaciju citoskeleta i sinaptičku reorganizaciju. Gledano u celini, rezultati ove studije su ukazali da bolje razumevanje bioloških posledica primene propofola u juvenilnom/pubertetskom uzrastu može doprineti sagledavanju starosno zavisnih zdravstvenih rizika tretmana, koji su do sada intenzivno istraživani samo u ekstremima životne dobi, tj. u ranoj postnatalnoj i kasnoj životnoj fazi., Propofol is a commonly used anesthetic in modern medicine. Addictive potential of propofol is observed, as well as the impact on the memory process. However, both phenomena are still insufficiently explored. Bearing in mind that adolescence is a period of extreme sensitivity to addictive substances and intense maturation of the mnemonic potential, the aim of this study was to examine the effect of a single exposure to propofol anesthesia, which is a typical method of its clinical application, on dopaminergic signaling, synaptic and neuronal activity in different brain regions and behavior of juvenile/peripubertal rats, as a model system of human periadolescencent development. The effects were analyzed 4, 24 and 48 hours after the treatment, in male Wistar rats aged 35 days.
The obtained findings for the first time showed that exposure to propofol anesthesia caused changes in the expression/phosphorylation of signal molecules that are already recognized as significant for the action of the addictive substances. Of all the analyzed dopaminoceptive brain regions (medial prefrontal cortex, striatum and thalamus), significant changes in the expression of the phosphorylated/activated form of DARPP-32 protein, indicator of postsynaptic dopaminergic signalling, were observed only in the thalamus, 4 and 24 hours after the treatment, and were accompanied by increased expression of FosB/ΔFosB protein, a biochemical indicator of neuronal activity. The alterations were localized in the paraventricular thalamic nucleus and the mid-dorsal thalamus. An increase in the expression of the phosphorylated form of CaMKIIα, a biochemical sensor of synaptic activity that has an important role in memory on addictive substances exposure, was detected in striatum and medial prefrontal cortex. Reduction in the intensity of anxiety (estimated in accordence to the data obtained in the light/dark box and elevated plus maze tests) was observed 24 hours after the treatment, along with the decrease in the expression of FosB protein in striatum, which can be interpreted as a sensation seeking due to decreased activity of brain region important for a pleasure/motivation. An enhanced motor response to d-amphetamine and phencyclidine was observed 24 hours after the treatment (cross
sensitization), as confirmation that despite the different primary mechanisms of action, propofol and these two drugs use the same neuronal pathways to achieve psychomotor effects. The results of the study also indicated that in peripubertal rats exposed to propofol anesthesia, there were some difficulties in memory retrieval and acquisition of new learning in non-aversive memory tests (spatial habituation, novel object recognition), 24 hours after the treatment. These effects were accompanied by changes in the expression of molecules involved in retrieval and reconsolidation of episodic memory (BDNF/TrkB, Egr-1, ERK1/2, CaMKIIα, FosB/ΔFosB) in the dorsal hippocampus. Detected difficulties in recognizing the novelty are consistent with a few clinical studies that emphasize the importance of controlling basic cognitive functions in older children after exposure to propofol anesthesia. Also, in all investigated brain regions, except the thalamus, an increase in the expression of the phosphorylated form of Adducinβ protein was observed, which is an indication of the cytoskelet destabilization and synaptic reorganization.
Overall, the results of this study accentuated that a better understanding of the biological consequences of the use of propofol in juvenile/puberty period could contribute to the undestanding of age-dependent health risks of the treatment, which have been extensively investigated so far only at the extremes of age, i.e. during early postnatal period and in aging.",
publisher = "Belgrade: University of Belgrade, Faculty of Biology",
journal = "University of Belgrade, Faculty of Biology",
title = "Efekti anestezije indukovane propofolom na sinaptičku plastičnost, aktivnost dopaminskog sistema i ponašanje juvenilnih pacova, The effects of propofol induced anesthesia on synaptic plasticity, dopaminergic system activity and behaviour of juvenile rats",
pages = "1-156",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3137"
}

Pavković, Ž.. (2018). Efekti anestezije indukovane propofolom na sinaptičku plastičnost, aktivnost dopaminskog sistema i ponašanje juvenilnih pacova. in University of Belgrade, Faculty of Biology
Belgrade: University of Belgrade, Faculty of Biology., 1-156.
https://hdl.handle.net/21.15107/rcub_ibiss_3137

Pavković Ž. Efekti anestezije indukovane propofolom na sinaptičku plastičnost, aktivnost dopaminskog sistema i ponašanje juvenilnih pacova. in University of Belgrade, Faculty of Biology. 2018;:1-156.
https://hdl.handle.net/21.15107/rcub_ibiss_3137 .

Pavković, Željko, "Efekti anestezije indukovane propofolom na sinaptičku plastičnost, aktivnost dopaminskog sistema i ponašanje juvenilnih pacova" in University of Belgrade, Faculty of Biology (2018):1-156,
https://hdl.handle.net/21.15107/rcub_ibiss_3137 .

TY  - JOUR
AU  - Pavković, Željko
AU  - Milanović, Desanka
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
AU  - Pešić, Vesna
PY  - 2018
UR  - http://doi.wiley.com/10.1111/pan.13396
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29752843
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3061
AB  - BACKGROUND The effects of anesthetic drugs on postoperative cognitive function in children are not well defined and have not been experimentally addressed. AIMS The present study aimed to examine the influence of propofol anesthesia exposure on nonaversive hippocampus-dependent learning and biochemical changes involved in memory process in the dorsal hippocampus, in peripubertal rats as the rodent model of periadolescence. METHODS The intersession spatial habituation and the novel object recognition tasks were used to assess spatial and nonspatial, nonaversive hippocampus-dependent learning. The exposure to anesthesia was performed after comparably long acquisition phases in both tasks. Behavioral testing lasted for 2 consecutive days (24-hour retention period). Changes in the expression of molecules involved in memory retrieval/reconsolidation were examined in the dorsal hippocampus by Western blot and immunohistochemistry, at the time of behavioral testing. RESULTS Exposure to propofol anesthesia resulted in inappropriate assessment of spatial novelty at the beginning of the test session and affected continuation of acquisition in the spatial habituation test. The treatment did not affect recognition of the novel object at the beginning of the test session but it attenuated overall preference to novelty, reflecting retrieval of a weak memory. The expression of phosphorylated extracellular signal-regulated kinase 2 (involved in memory retrieval) was decreased while the level of phosphorylated Ca2+ /calmodulin-dependent protein kinase IIα and early growth response protein 1 (involved in memory reconsolidation) was increased in the dorsal hippocampus. The level of Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog B (neuronal activity indicator) was increased in the dorsal dentate gyrus. Enhanced exploratory activity was still evident in the propofol anesthesia exposure (PAE) group 48 hour after the treatment in both tasks. CONCLUSION In peripubertal rats, propofol anesthesia exposure affects memory retrieval and acquisition of new learning in the spatial and nonspatial, nonaversive learning tasks 24 hour after the treatment, along with the expression of molecules that participate in memory retrieval/reconsolidation in the dorsal hippocampus. These results may have clinical implications, favoring control of basic cognitive functions in older children after the propofol exposure.
T2  - Paediatric Anaesthesia
T1  - The influence of propofol anesthesia exposure on nonaversive memory retrieval and expression of molecules involved in memory process in the dorsal hippocampus in peripubertal rats.
IS  - 6
VL  - 28
DO  - 10.1111/pan.13396
SP  - 537
EP  - 546
ER  - 

@article{
author = "Pavković, Željko and Milanović, Desanka and Ruždijić, Sabera and Kanazir, Selma and Pešić, Vesna",
year = "2018",
abstract = "BACKGROUND The effects of anesthetic drugs on postoperative cognitive function in children are not well defined and have not been experimentally addressed. AIMS The present study aimed to examine the influence of propofol anesthesia exposure on nonaversive hippocampus-dependent learning and biochemical changes involved in memory process in the dorsal hippocampus, in peripubertal rats as the rodent model of periadolescence. METHODS The intersession spatial habituation and the novel object recognition tasks were used to assess spatial and nonspatial, nonaversive hippocampus-dependent learning. The exposure to anesthesia was performed after comparably long acquisition phases in both tasks. Behavioral testing lasted for 2 consecutive days (24-hour retention period). Changes in the expression of molecules involved in memory retrieval/reconsolidation were examined in the dorsal hippocampus by Western blot and immunohistochemistry, at the time of behavioral testing. RESULTS Exposure to propofol anesthesia resulted in inappropriate assessment of spatial novelty at the beginning of the test session and affected continuation of acquisition in the spatial habituation test. The treatment did not affect recognition of the novel object at the beginning of the test session but it attenuated overall preference to novelty, reflecting retrieval of a weak memory. The expression of phosphorylated extracellular signal-regulated kinase 2 (involved in memory retrieval) was decreased while the level of phosphorylated Ca2+ /calmodulin-dependent protein kinase IIα and early growth response protein 1 (involved in memory reconsolidation) was increased in the dorsal hippocampus. The level of Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog B (neuronal activity indicator) was increased in the dorsal dentate gyrus. Enhanced exploratory activity was still evident in the propofol anesthesia exposure (PAE) group 48 hour after the treatment in both tasks. CONCLUSION In peripubertal rats, propofol anesthesia exposure affects memory retrieval and acquisition of new learning in the spatial and nonspatial, nonaversive learning tasks 24 hour after the treatment, along with the expression of molecules that participate in memory retrieval/reconsolidation in the dorsal hippocampus. These results may have clinical implications, favoring control of basic cognitive functions in older children after the propofol exposure.",
journal = "Paediatric Anaesthesia",
title = "The influence of propofol anesthesia exposure on nonaversive memory retrieval and expression of molecules involved in memory process in the dorsal hippocampus in peripubertal rats.",
number = "6",
volume = "28",
doi = "10.1111/pan.13396",
pages = "537-546"
}

Pavković, Ž., Milanović, D., Ruždijić, S., Kanazir, S.,& Pešić, V.. (2018). The influence of propofol anesthesia exposure on nonaversive memory retrieval and expression of molecules involved in memory process in the dorsal hippocampus in peripubertal rats.. in Paediatric Anaesthesia, 28(6), 537-546.
https://doi.org/10.1111/pan.13396

Pavković Ž, Milanović D, Ruždijić S, Kanazir S, Pešić V. The influence of propofol anesthesia exposure on nonaversive memory retrieval and expression of molecules involved in memory process in the dorsal hippocampus in peripubertal rats.. in Paediatric Anaesthesia. 2018;28(6):537-546.
doi:10.1111/pan.13396 .

Pavković, Željko, Milanović, Desanka, Ruždijić, Sabera, Kanazir, Selma, Pešić, Vesna, "The influence of propofol anesthesia exposure on nonaversive memory retrieval and expression of molecules involved in memory process in the dorsal hippocampus in peripubertal rats." in Paediatric Anaesthesia, 28, no. 6 (2018):537-546,
https://doi.org/10.1111/pan.13396 . .

TY  - JOUR
AU  - Pavković, Željko
AU  - Smiljanić, Kosara
AU  - Kanazir, Selma
AU  - Milanović, Desanka
AU  - Pešić, Vesna
AU  - Ruždijić, Sabera
PY  - 2017
UR  - http://doi.wiley.com/10.1111/pan.13182
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2831
AB  - Background: Propofol is commonly used in modern anesthesiology. Some findings suggest that it is highly addictive. Aim: In this study it was examined whether propofol anesthesia exposure was able to induce behavioral alterations and brain molecular changes already described in addictive drug usage in peripubertal rats, during the onset of mid/periadolescence as a developmental period with increasing vulnerability to drug addiction. Methods: The expression of D1 dopamine receptor, a dopamine, and cAMP-regulated phosphoprotein with a Mr 32 000; Ca 2+ /calmodulin-dependent protein kinase IIα; and Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog-B was examined in peripubertal rats 4, 24, and 48 hour after propofol anesthesia exposure by Western blot and immunohistochemistry. Brain regions of interest were the medial prefrontal cortex, the striatum, and the thalamus. Anxiety and behavioral cross-sensitization to d-amphetamine were examined as well. Results: Significant increase in the expression of dopamine and cAMP-regulated phosphoprotein with a Mr 32 000 phosphorylated at threonine 34, a postsynaptic marker of dopaminergic neurotransmission, and Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog-B, a marker of neuronal activity, was detected in the thalamus of experimental animals 4-24 hour after the treatment, with the accent on the paraventricular thalamic nucleus. Significant increase in the expression of Ca 2+ /calmodulin-dependent protein kinase IIα phosphorylated at threonine 286, a sensor of synaptic activity, was observed in the prefrontal cortex and the striatum 24 hour after propofol anesthesia exposure. It was accompanied by a significant decrease in Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog-B expression in the striatum. Decreased behavioral inhibition in aversive environment and increased motor response to d-amphetamine in a context-independent manner were observed as well. Conclusion: In peripubertal rats, propofol anesthesia exposure induces transient molecular and behavioral response that share similarities with those reported previously for addictive drugs. In the absence of additional pharmacological manipulation, all detected effects receded within 48 hour after the treatment.
T2  - Pediatric Anesthesia
T1  - Brain molecular changes and behavioral alterations induced by propofol anesthesia exposure in peripubertal rats
IS  - 9
VL  - 27
DO  - 10.1111/pan.13182
SP  - 962
EP  - 972
ER  - 

@article{
author = "Pavković, Željko and Smiljanić, Kosara and Kanazir, Selma and Milanović, Desanka and Pešić, Vesna and Ruždijić, Sabera",
year = "2017",
abstract = "Background: Propofol is commonly used in modern anesthesiology. Some findings suggest that it is highly addictive. Aim: In this study it was examined whether propofol anesthesia exposure was able to induce behavioral alterations and brain molecular changes already described in addictive drug usage in peripubertal rats, during the onset of mid/periadolescence as a developmental period with increasing vulnerability to drug addiction. Methods: The expression of D1 dopamine receptor, a dopamine, and cAMP-regulated phosphoprotein with a Mr 32 000; Ca 2+ /calmodulin-dependent protein kinase IIα; and Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog-B was examined in peripubertal rats 4, 24, and 48 hour after propofol anesthesia exposure by Western blot and immunohistochemistry. Brain regions of interest were the medial prefrontal cortex, the striatum, and the thalamus. Anxiety and behavioral cross-sensitization to d-amphetamine were examined as well. Results: Significant increase in the expression of dopamine and cAMP-regulated phosphoprotein with a Mr 32 000 phosphorylated at threonine 34, a postsynaptic marker of dopaminergic neurotransmission, and Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog-B, a marker of neuronal activity, was detected in the thalamus of experimental animals 4-24 hour after the treatment, with the accent on the paraventricular thalamic nucleus. Significant increase in the expression of Ca 2+ /calmodulin-dependent protein kinase IIα phosphorylated at threonine 286, a sensor of synaptic activity, was observed in the prefrontal cortex and the striatum 24 hour after propofol anesthesia exposure. It was accompanied by a significant decrease in Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog-B expression in the striatum. Decreased behavioral inhibition in aversive environment and increased motor response to d-amphetamine in a context-independent manner were observed as well. Conclusion: In peripubertal rats, propofol anesthesia exposure induces transient molecular and behavioral response that share similarities with those reported previously for addictive drugs. In the absence of additional pharmacological manipulation, all detected effects receded within 48 hour after the treatment.",
journal = "Pediatric Anesthesia",
title = "Brain molecular changes and behavioral alterations induced by propofol anesthesia exposure in peripubertal rats",
number = "9",
volume = "27",
doi = "10.1111/pan.13182",
pages = "962-972"
}

Pavković, Ž., Smiljanić, K., Kanazir, S., Milanović, D., Pešić, V.,& Ruždijić, S.. (2017). Brain molecular changes and behavioral alterations induced by propofol anesthesia exposure in peripubertal rats. in Pediatric Anesthesia, 27(9), 962-972.
https://doi.org/10.1111/pan.13182

Pavković Ž, Smiljanić K, Kanazir S, Milanović D, Pešić V, Ruždijić S. Brain molecular changes and behavioral alterations induced by propofol anesthesia exposure in peripubertal rats. in Pediatric Anesthesia. 2017;27(9):962-972.
doi:10.1111/pan.13182 .

Pavković, Željko, Smiljanić, Kosara, Kanazir, Selma, Milanović, Desanka, Pešić, Vesna, Ruždijić, Sabera, "Brain molecular changes and behavioral alterations induced by propofol anesthesia exposure in peripubertal rats" in Pediatric Anesthesia, 27, no. 9 (2017):962-972,
https://doi.org/10.1111/pan.13182 . .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Pešić, Vesna
AU  - Pavković, Željko
AU  - Martać, Ljiljana
AU  - Kanazir, Selma
AU  - Filipović, Ljupka
AU  - Sekulić, Slobodan
PY  - 2016
UR  - http://www.journals.elsevier.com/behavioural-brain-research/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5456
AB  - Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.
PB  - Amsterdam : Elsevier
T2  - Behavioural Brain Research
T1  - Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development
VL  - 311
DO  - 10.1016/j.bbr.2016.05.023
SP  - 99
EP  - 109
ER  - 

@article{
author = "Podgorac, Jelena and Pešić, Vesna and Pavković, Željko and Martać, Ljiljana and Kanazir, Selma and Filipović, Ljupka and Sekulić, Slobodan",
year = "2016",
abstract = "Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.",
publisher = "Amsterdam : Elsevier",
journal = "Behavioural Brain Research",
title = "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development",
volume = "311",
doi = "10.1016/j.bbr.2016.05.023",
pages = "99-109"
}

Podgorac, J., Pešić, V., Pavković, Ž., Martać, L., Kanazir, S., Filipović, L.,& Sekulić, S.. (2016). Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research
Amsterdam : Elsevier., 311, 99-109.
https://doi.org/10.1016/j.bbr.2016.05.023

Podgorac J, Pešić V, Pavković Ž, Martać L, Kanazir S, Filipović L, Sekulić S. Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research. 2016;311:99-109.
doi:10.1016/j.bbr.2016.05.023 .

Podgorac, Jelena, Pešić, Vesna, Pavković, Željko, Martać, Ljiljana, Kanazir, Selma, Filipović, Ljupka, Sekulić, Slobodan, "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development" in Behavioural Brain Research, 311 (2016):99-109,
https://doi.org/10.1016/j.bbr.2016.05.023 . .

TY  - JOUR
AU  - Milanović, Desanka
AU  - Pešić, Vesna
AU  - Lončarević-Vasiljković, Nataša
AU  - Pavković, Željko
AU  - Popić, Jelena
AU  - Kanazir, Selma
AU  - Jevtović-Todorović, Vesna
AU  - Ruždijić, Sabera
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5457
AB  - A number of experimental studies have reported that exposure to common, clinically used anesthetics induce extensive neuroapoptosis and cognitive impairment when applied to young rodents, up to 2 weeks old, in phase of rapid synaptogenesis. Propofol is the most used general anesthetic in clinical practice whose mechanisms of neurotoxicity on the developing brain remains to be examined in depth. This study investigated effects of different exposures to propofol anesthesia on Fas receptor and Fas ligand expressions, which mediate proapoptotic and proinflammation signaling in the brain. Propofol (20 mg/kg) was administered to 7-day-old rats in multiple doses sufficient to maintain 2-, 4- and 6-h duration of anesthesia. Animals were sacrificed at 0, 4, 16 and 24 h after termination of anesthesia. It was found that propofol anesthesia induced Fas/FasL and downstream caspase-8 expression more prominently in the thalamus than in the cortex. Opposite, Bcl-2 and caspase-9, markers of intrinsic pathway activation, were shown to be more influenced by propofol treatment in the cortex. Further, we have established upregulation of caspase-1 and IL-1β cytokine transcription as well as subsequent activation of microglia that is potentially associated with brain inflammation. Behavioral analyses revealed that P35 and P60 animals, neonatally exposed to propofol, had significantly higher motor activity during three consecutive days of testing in the open field, though formation of the intersession habituation was not prevented. This data, together with our previous results, contributes to elucidation of complex mechanisms of propofol toxicity in developing brain.
PB  - New York: Springer
T2  - Neurotoxicity Research
T1  - The Fas Ligand/Fas Death Receptor Pathways Contribute to Propofol-Induced Apoptosis and Neuroinflammation in the Brain of Neonatal Rats
IS  - 3
VL  - 30
DO  - 10.1007/s12640-016-9629-1
SP  - 434
EP  - 452
ER  - 

@article{
author = "Milanović, Desanka and Pešić, Vesna and Lončarević-Vasiljković, Nataša and Pavković, Željko and Popić, Jelena and Kanazir, Selma and Jevtović-Todorović, Vesna and Ruždijić, Sabera",
year = "2016",
abstract = "A number of experimental studies have reported that exposure to common, clinically used anesthetics induce extensive neuroapoptosis and cognitive impairment when applied to young rodents, up to 2 weeks old, in phase of rapid synaptogenesis. Propofol is the most used general anesthetic in clinical practice whose mechanisms of neurotoxicity on the developing brain remains to be examined in depth. This study investigated effects of different exposures to propofol anesthesia on Fas receptor and Fas ligand expressions, which mediate proapoptotic and proinflammation signaling in the brain. Propofol (20 mg/kg) was administered to 7-day-old rats in multiple doses sufficient to maintain 2-, 4- and 6-h duration of anesthesia. Animals were sacrificed at 0, 4, 16 and 24 h after termination of anesthesia. It was found that propofol anesthesia induced Fas/FasL and downstream caspase-8 expression more prominently in the thalamus than in the cortex. Opposite, Bcl-2 and caspase-9, markers of intrinsic pathway activation, were shown to be more influenced by propofol treatment in the cortex. Further, we have established upregulation of caspase-1 and IL-1β cytokine transcription as well as subsequent activation of microglia that is potentially associated with brain inflammation. Behavioral analyses revealed that P35 and P60 animals, neonatally exposed to propofol, had significantly higher motor activity during three consecutive days of testing in the open field, though formation of the intersession habituation was not prevented. This data, together with our previous results, contributes to elucidation of complex mechanisms of propofol toxicity in developing brain.",
publisher = "New York: Springer",
journal = "Neurotoxicity Research",
title = "The Fas Ligand/Fas Death Receptor Pathways Contribute to Propofol-Induced Apoptosis and Neuroinflammation in the Brain of Neonatal Rats",
number = "3",
volume = "30",
doi = "10.1007/s12640-016-9629-1",
pages = "434-452"
}

Milanović, D., Pešić, V., Lončarević-Vasiljković, N., Pavković, Ž., Popić, J., Kanazir, S., Jevtović-Todorović, V.,& Ruždijić, S.. (2016). The Fas Ligand/Fas Death Receptor Pathways Contribute to Propofol-Induced Apoptosis and Neuroinflammation in the Brain of Neonatal Rats. in Neurotoxicity Research
New York: Springer., 30(3), 434-452.
https://doi.org/10.1007/s12640-016-9629-1

Milanović D, Pešić V, Lončarević-Vasiljković N, Pavković Ž, Popić J, Kanazir S, Jevtović-Todorović V, Ruždijić S. The Fas Ligand/Fas Death Receptor Pathways Contribute to Propofol-Induced Apoptosis and Neuroinflammation in the Brain of Neonatal Rats. in Neurotoxicity Research. 2016;30(3):434-452.
doi:10.1007/s12640-016-9629-1 .

Milanović, Desanka, Pešić, Vesna, Lončarević-Vasiljković, Nataša, Pavković, Željko, Popić, Jelena, Kanazir, Selma, Jevtović-Todorović, Vesna, Ruždijić, Sabera, "The Fas Ligand/Fas Death Receptor Pathways Contribute to Propofol-Induced Apoptosis and Neuroinflammation in the Brain of Neonatal Rats" in Neurotoxicity Research, 30, no. 3 (2016):434-452,
https://doi.org/10.1007/s12640-016-9629-1 . .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Pešić, Vesna
AU  - Pavković, Željko
AU  - Martać, Ljiljana
AU  - Kanazir, Selma
AU  - Filipović, Ljupka
AU  - Sekulić, Slobodan
PY  - 2016
UR  - http://www.journals.elsevier.com/behavioural-brain-research/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5455
AB  - Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.
PB  - Amsterdam : Elsevier
T2  - Behavioural Brain Research
T1  - Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development
VL  - 311
DO  - 10.1016/j.bbr.2016.05.023
SP  - 99
EP  - 109
ER  - 

@article{
author = "Podgorac, Jelena and Pešić, Vesna and Pavković, Željko and Martać, Ljiljana and Kanazir, Selma and Filipović, Ljupka and Sekulić, Slobodan",
year = "2016",
abstract = "Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.",
publisher = "Amsterdam : Elsevier",
journal = "Behavioural Brain Research",
title = "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development",
volume = "311",
doi = "10.1016/j.bbr.2016.05.023",
pages = "99-109"
}

Podgorac, J., Pešić, V., Pavković, Ž., Martać, L., Kanazir, S., Filipović, L.,& Sekulić, S.. (2016). Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research
Amsterdam : Elsevier., 311, 99-109.
https://doi.org/10.1016/j.bbr.2016.05.023

Podgorac J, Pešić V, Pavković Ž, Martać L, Kanazir S, Filipović L, Sekulić S. Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research. 2016;311:99-109.
doi:10.1016/j.bbr.2016.05.023 .

Podgorac, Jelena, Pešić, Vesna, Pavković, Željko, Martać, Ljiljana, Kanazir, Selma, Filipović, Ljupka, Sekulić, Slobodan, "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development" in Behavioural Brain Research, 311 (2016):99-109,
https://doi.org/10.1016/j.bbr.2016.05.023 . .

TY  - JOUR
AU  - Stojković Burić, Sonja
AU  - Podolski-Renić, Ana
AU  - Dinić, Jelena
AU  - Pavković, Željko
AU  - Ayuso, Jose M.
AU  - Fernández, Luis J.
AU  - Ochoa, Ignacio
AU  - Pérez-Martínez, Víctor Manuel
AU  - Pešić, Vesna
AU  - Pešić, Milica
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/123456789/3872
AB  - Chemoresistance and invasion properties are severe limitations to efficient glioma therapy. Therefore, development of glioma in vivo models that more accurately resemble the situation observed in patients emerges. Previously, we established RC6 rat glioma cell line resistant to DNA damaging agents including antiglioma approved therapies such as 3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and temozolomide (TMZ). Herein, we evaluated the invasiveness of RC6 cells in vitro and in a new orthotopic animal model. For comparison, we used C6 cells from which RC6 cells originated. Differences in cell growth properties were assessed by real-time cell analyzer. Cells’ invasive potential in vitro was studied in fluorescently labeled gelatin and by formation of multicellular spheroids in hydrogel. For animal studies, fluorescently labeled cells were inoculated into adult male Wistar rat brains. Consecutive coronal and sagittal brain sections were analyzed 10 and 25 days post-inoculation, while rats’ behavior was recorded during three days in the open field test starting from 25th day post-inoculation. We demonstrated that development of chemoresistance induced invasive phenotype of RC6 cells with significant behavioral impediments implying usefulness of orthotopic RC6 glioma allograft in preclinical studies for the examination of new approaches to counteract both chemoresistance and invasion of glioma cells.
PB  - Basel : MDPI
T2  - Molecules
T1  - Resistance to DNA Damaging Agents Produced Invasive Phenotype of Rat Glioma Cells—Characterization of a New in Vivo Model
IS  - 7
VL  - 21
DO  - 10.3390/molecules21070843
SP  - 843
ER  - 

@article{
author = "Stojković Burić, Sonja and Podolski-Renić, Ana and Dinić, Jelena and Pavković, Željko and Ayuso, Jose M. and Fernández, Luis J. and Ochoa, Ignacio and Pérez-Martínez, Víctor Manuel and Pešić, Vesna and Pešić, Milica",
year = "2016",
abstract = "Chemoresistance and invasion properties are severe limitations to efficient glioma therapy. Therefore, development of glioma in vivo models that more accurately resemble the situation observed in patients emerges. Previously, we established RC6 rat glioma cell line resistant to DNA damaging agents including antiglioma approved therapies such as 3-bis(2-chloroethyl)-1-nitrosourea (BCNU) and temozolomide (TMZ). Herein, we evaluated the invasiveness of RC6 cells in vitro and in a new orthotopic animal model. For comparison, we used C6 cells from which RC6 cells originated. Differences in cell growth properties were assessed by real-time cell analyzer. Cells’ invasive potential in vitro was studied in fluorescently labeled gelatin and by formation of multicellular spheroids in hydrogel. For animal studies, fluorescently labeled cells were inoculated into adult male Wistar rat brains. Consecutive coronal and sagittal brain sections were analyzed 10 and 25 days post-inoculation, while rats’ behavior was recorded during three days in the open field test starting from 25th day post-inoculation. We demonstrated that development of chemoresistance induced invasive phenotype of RC6 cells with significant behavioral impediments implying usefulness of orthotopic RC6 glioma allograft in preclinical studies for the examination of new approaches to counteract both chemoresistance and invasion of glioma cells.",
publisher = "Basel : MDPI",
journal = "Molecules",
title = "Resistance to DNA Damaging Agents Produced Invasive Phenotype of Rat Glioma Cells—Characterization of a New in Vivo Model",
number = "7",
volume = "21",
doi = "10.3390/molecules21070843",
pages = "843"
}

Stojković Burić, S., Podolski-Renić, A., Dinić, J., Pavković, Ž., Ayuso, J. M., Fernández, L. J., Ochoa, I., Pérez-Martínez, V. M., Pešić, V.,& Pešić, M.. (2016). Resistance to DNA Damaging Agents Produced Invasive Phenotype of Rat Glioma Cells—Characterization of a New in Vivo Model. in Molecules
Basel : MDPI., 21(7), 843.
https://doi.org/10.3390/molecules21070843

Stojković Burić S, Podolski-Renić A, Dinić J, Pavković Ž, Ayuso JM, Fernández LJ, Ochoa I, Pérez-Martínez VM, Pešić V, Pešić M. Resistance to DNA Damaging Agents Produced Invasive Phenotype of Rat Glioma Cells—Characterization of a New in Vivo Model. in Molecules. 2016;21(7):843.
doi:10.3390/molecules21070843 .

Stojković Burić, Sonja, Podolski-Renić, Ana, Dinić, Jelena, Pavković, Željko, Ayuso, Jose M., Fernández, Luis J., Ochoa, Ignacio, Pérez-Martínez, Víctor Manuel, Pešić, Vesna, Pešić, Milica, "Resistance to DNA Damaging Agents Produced Invasive Phenotype of Rat Glioma Cells—Characterization of a New in Vivo Model" in Molecules, 21, no. 7 (2016):843,
https://doi.org/10.3390/molecules21070843 . .

TY  - JOUR
AU  - Smiljanić, Kosara
AU  - Pesic, Vesna
AU  - Mladenović, Aleksandra
AU  - Pavković, Željko
AU  - Brkić, Marjana
AU  - Ruždijić, Sabera
AU  - Kanazir, Selma
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2013
AB  - Dietary restriction (DR) exerts significant beneficial effects in terms
   of aging and age-related diseases in many organisms including humans.
   The present study aimed to examine the influence of long-term DR on the
   BDNF system at the transcriptional and translational levels in the
   cortex and hippocampus of middle-aged (12-month-old) and aged
   (24-month-old) male Wistar rats. The obtained results revealed that the
   DR upregulated the expression of exon-specific BDNF transcripts in both
   regions, followed by elevated levels of mBDNF only in the cortex in
   middle-aged animals. In aged animals, DR modulated BDNF protein levels
   by increasing proBDNF and by declining mBDNF levels. Additionally,
   elevated levels of the full-length TrkB accompanied by a decreased level
   of the less-glycosylated TrkB protein were observed in middle-aged rats
   following DR, while in aged rats, DR amplified only the expression of
   the less-glycosylated form of TrkB. The levels of phosphorylated
   TrkB(Y816) were stable during aging regardless of feeding. Reduced
   levels of p75(NTR) were detected in both regions of middle-aged DR-fed
   animals, while a significant increase was measured in the cortex of aged
   DR-fed rats. These findings shed additional light on DR as a modulator
   of BDNF system revealing its disparate effects in middle-aged and aged
   animals. Given the importance of the proBDNF/BDNF circuit-level
   expression in different brain functions and various aspects of behavior,
   it is necessary to further elucidate the optimal duration of the applied
   dietary regimen with regard to the animal age in order to achieve its
   most favorable effects.
T2  - Biogerontology
T1  - Long-term dietary restriction differentially affects the expression of
 BDNF and its receptors in the cortex and hippocampus of middle-aged and
 aged male rats
IS  - 1
VL  - 16
DO  - 10.1007/s10522-014-9537-9
SP  - 71
EP  - 83
ER  - 

@article{
author = "Smiljanić, Kosara and Pesic, Vesna and Mladenović, Aleksandra and Pavković, Željko and Brkić, Marjana and Ruždijić, Sabera and Kanazir, Selma",
year = "2015",
abstract = "Dietary restriction (DR) exerts significant beneficial effects in terms
   of aging and age-related diseases in many organisms including humans.
   The present study aimed to examine the influence of long-term DR on the
   BDNF system at the transcriptional and translational levels in the
   cortex and hippocampus of middle-aged (12-month-old) and aged
   (24-month-old) male Wistar rats. The obtained results revealed that the
   DR upregulated the expression of exon-specific BDNF transcripts in both
   regions, followed by elevated levels of mBDNF only in the cortex in
   middle-aged animals. In aged animals, DR modulated BDNF protein levels
   by increasing proBDNF and by declining mBDNF levels. Additionally,
   elevated levels of the full-length TrkB accompanied by a decreased level
   of the less-glycosylated TrkB protein were observed in middle-aged rats
   following DR, while in aged rats, DR amplified only the expression of
   the less-glycosylated form of TrkB. The levels of phosphorylated
   TrkB(Y816) were stable during aging regardless of feeding. Reduced
   levels of p75(NTR) were detected in both regions of middle-aged DR-fed
   animals, while a significant increase was measured in the cortex of aged
   DR-fed rats. These findings shed additional light on DR as a modulator
   of BDNF system revealing its disparate effects in middle-aged and aged
   animals. Given the importance of the proBDNF/BDNF circuit-level
   expression in different brain functions and various aspects of behavior,
   it is necessary to further elucidate the optimal duration of the applied
   dietary regimen with regard to the animal age in order to achieve its
   most favorable effects.",
journal = "Biogerontology",
title = "Long-term dietary restriction differentially affects the expression of
 BDNF and its receptors in the cortex and hippocampus of middle-aged and
 aged male rats",
number = "1",
volume = "16",
doi = "10.1007/s10522-014-9537-9",
pages = "71-83"
}

Smiljanić, K., Pesic, V., Mladenović, A., Pavković, Ž., Brkić, M., Ruždijić, S.,& Kanazir, S.. (2015). Long-term dietary restriction differentially affects the expression of
 BDNF and its receptors in the cortex and hippocampus of middle-aged and
 aged male rats. in Biogerontology, 16(1), 71-83.
https://doi.org/10.1007/s10522-014-9537-9

Smiljanić K, Pesic V, Mladenović A, Pavković Ž, Brkić M, Ruždijić S, Kanazir S. Long-term dietary restriction differentially affects the expression of
 BDNF and its receptors in the cortex and hippocampus of middle-aged and
 aged male rats. in Biogerontology. 2015;16(1):71-83.
doi:10.1007/s10522-014-9537-9 .

Smiljanić, Kosara, Pesic, Vesna, Mladenović, Aleksandra, Pavković, Željko, Brkić, Marjana, Ruždijić, Sabera, Kanazir, Selma, "Long-term dietary restriction differentially affects the expression of
 BDNF and its receptors in the cortex and hippocampus of middle-aged and
 aged male rats" in Biogerontology, 16, no. 1 (2015):71-83,
https://doi.org/10.1007/s10522-014-9537-9 . .