TY  - JOUR
AU  - Đurović, Jelena
AU  - Stamenković, Gorana
AU  - Todorović, Jelena
AU  - Aleksić, Natasa
AU  - Stojković, Oliver
PY  - 2020
UR  - https://www.tandfonline.com/doi/full/10.1080/14647273.2018.1515503
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3141
AB  - AbstractUnexplained infertility refers to the absence of a definable cause of reproductive failure. Vitamin D receptor (VDR) acts as a transcription factor and regulates a number of vitamin D-responsive genes, including those involved in the immune system. Recent finding that VDR is expressed in reproductive tissues suggests a possible importance of vitamin D in pregnancy. We conducted a case-control study to examine the association of polymorphisms in VDR gene with reproductive success. DNA from 117 female patients with unexplained infertility and 130 fertile controls was isolated from peripheral blood and VDR genotypes (FokI, BsmI, ApaI and TaqI) were detected by PCR-RFLP. Haplotypes were determined using Haploview software. Our results show significant association of FokI and BsmI polymorphisms with infertility (p < 0.05). The haplotype analysis confirmed strong linkage disequilibrium between closely positioned BsmI, ApaI and TaqI polymorphisms. Two haplotypes were associated with infertility: (i) hapl...
T2  - Human Fertility
T1  - Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility
IS  - 2
VL  - 23
DO  - 10.1080/14647273.2018.1515503
SP  - 101
SP  - 101
EP  - 110
EP  - 110
ER  - 

@article{
author = "Đurović, Jelena and Stamenković, Gorana and Todorović, Jelena and Aleksić, Natasa and Stojković, Oliver",
year = "2020",
abstract = "AbstractUnexplained infertility refers to the absence of a definable cause of reproductive failure. Vitamin D receptor (VDR) acts as a transcription factor and regulates a number of vitamin D-responsive genes, including those involved in the immune system. Recent finding that VDR is expressed in reproductive tissues suggests a possible importance of vitamin D in pregnancy. We conducted a case-control study to examine the association of polymorphisms in VDR gene with reproductive success. DNA from 117 female patients with unexplained infertility and 130 fertile controls was isolated from peripheral blood and VDR genotypes (FokI, BsmI, ApaI and TaqI) were detected by PCR-RFLP. Haplotypes were determined using Haploview software. Our results show significant association of FokI and BsmI polymorphisms with infertility (p < 0.05). The haplotype analysis confirmed strong linkage disequilibrium between closely positioned BsmI, ApaI and TaqI polymorphisms. Two haplotypes were associated with infertility: (i) hapl...",
journal = "Human Fertility",
title = "Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility",
number = "2",
volume = "23",
doi = "10.1080/14647273.2018.1515503",
pages = "101-101-110-110"
}

Đurović, J., Stamenković, G., Todorović, J., Aleksić, N.,& Stojković, O.. (2020). Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility. in Human Fertility, 23(2), 101-110.
https://doi.org/10.1080/14647273.2018.1515503

Đurović J, Stamenković G, Todorović J, Aleksić N, Stojković O. Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility. in Human Fertility. 2020;23(2):101-110.
doi:10.1080/14647273.2018.1515503 .

Đurović, Jelena, Stamenković, Gorana, Todorović, Jelena, Aleksić, Natasa, Stojković, Oliver, "Polymorphisms and haplotypes in VDR gene are associated with female idiopathic infertility" in Human Fertility, 23, no. 2 (2020):101-110,
https://doi.org/10.1080/14647273.2018.1515503 . .

TY  - JOUR
AU  - Đurović, Jelena
AU  - Stojković, Oliver
AU  - Todorović, Jelena
AU  - Savić, Kristina
AU  - Stamenković, Gorana
PY  - 2017
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0534-00121702377D
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2904
AB  - Methylenetetrahydrofolate reductase (MTHFR) plays a critical role in the folate metabolism. The polymorphism 677C > T of the MTHFR gene, producing thermolabile enzyme with decreased function, is widely studied and associated with many conditions. Additionally, it has been shown that another polymorphism, 1298A > C, also reduces the activity of this enzyme, although to a lesser extent. The aim of this study is to evaluate the clinical informativeness of testing both MTHFR polymorphisms. Genomic DNA, were extracted from peripheral blood of 180 female patients with pregnancy complications and 183 healthy female controls, and genotyped for MTHFR 677C > T and 1298A > C loci, using TaqMan assays. Our study found similar frequency of alleles and genotypes between two groups. Based on MTHFR 677C > T genotype, 11.7% of patients homozygous for this mutation were under the possible risk. When the position 1298 was included in the testing, 22.8% of the patients were heterozygous for both polymorphisms. Additionally, 8.9% of the patients were homozygous only for the MTHFR 1298 mutation. Although, there was no differences compared to healthy control (p > 0.05), 43% of patients were found to have elevated risk which is about four time higher than results with only MTHFR 677C > T genotyping. After obtaining information for the 677 position, testing for the second polymorphism (1298A > C) should be considered, since we have shown that it dramatically increases the rate of detection of patients who are potentially at risk for MTHFR associated conditions.
T2  - Genetika
T2  - Genetika
T1  - Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?
IS  - 2
VL  - 49
DO  - 10.2298/GENSR1702377D
SP  - 377
EP  - 386
ER  - 

@article{
author = "Đurović, Jelena and Stojković, Oliver and Todorović, Jelena and Savić, Kristina and Stamenković, Gorana",
year = "2017",
abstract = "Methylenetetrahydrofolate reductase (MTHFR) plays a critical role in the folate metabolism. The polymorphism 677C > T of the MTHFR gene, producing thermolabile enzyme with decreased function, is widely studied and associated with many conditions. Additionally, it has been shown that another polymorphism, 1298A > C, also reduces the activity of this enzyme, although to a lesser extent. The aim of this study is to evaluate the clinical informativeness of testing both MTHFR polymorphisms. Genomic DNA, were extracted from peripheral blood of 180 female patients with pregnancy complications and 183 healthy female controls, and genotyped for MTHFR 677C > T and 1298A > C loci, using TaqMan assays. Our study found similar frequency of alleles and genotypes between two groups. Based on MTHFR 677C > T genotype, 11.7% of patients homozygous for this mutation were under the possible risk. When the position 1298 was included in the testing, 22.8% of the patients were heterozygous for both polymorphisms. Additionally, 8.9% of the patients were homozygous only for the MTHFR 1298 mutation. Although, there was no differences compared to healthy control (p > 0.05), 43% of patients were found to have elevated risk which is about four time higher than results with only MTHFR 677C > T genotyping. After obtaining information for the 677 position, testing for the second polymorphism (1298A > C) should be considered, since we have shown that it dramatically increases the rate of detection of patients who are potentially at risk for MTHFR associated conditions.",
journal = "Genetika, Genetika",
title = "Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?",
number = "2",
volume = "49",
doi = "10.2298/GENSR1702377D",
pages = "377-386"
}

Đurović, J., Stojković, O., Todorović, J., Savić, K.,& Stamenković, G.. (2017). Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?. in Genetika, 49(2), 377-386.
https://doi.org/10.2298/GENSR1702377D

Đurović J, Stojković O, Todorović J, Savić K, Stamenković G. Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?. in Genetika. 2017;49(2):377-386.
doi:10.2298/GENSR1702377D .

Đurović, Jelena, Stojković, Oliver, Todorović, Jelena, Savić, Kristina, Stamenković, Gorana, "Should MTHFR 1298 A>C be tested together with MTHFR 677 C>T polymorphism in women with reproductive challenges?" in Genetika, 49, no. 2 (2017):377-386,
https://doi.org/10.2298/GENSR1702377D . .

TY  - GEN
AU  - Đurović, Jelena
AU  - Stojković, Oliver
AU  - Todorović, Jelena
AU  - Brajić, Aleksandra
AU  - Stanković, Sanja
AU  - Obradović, Svetlana
AU  - Stamenković, Gorana
PY  - 2016
UR  - https://www.tandfonline.com/doi/full/10.1080/14647273.2016.1255785
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2595
AB  - Reproductive failure (recurrent foetal loss, unexplained infertility and IVF implantation failure) may be, in a number of cases, explained by thrombophilia, either acquired or inherited. Several genes contribute to thrombophilia, some with major effect (Factor V, Factor II), and some with minor effect (MTHFR, PAI-1, ATIII, etc.). The aim of this study was to estimate frequency of thrombophilia-associated genotypes (FII20210G > A, FV1691G > A, MTHFR677C > T and PAI-1 -675 4G/5G) in a group of 1631 Serbian women experiencing reproductive failure, and compare it with a healthy, female control group. Our results showed marginally significant (p = 0.050) differences in allele frequencies between patients and controls for the FV1691 mutations. For the FII20210G > A, although the statistical significance was not achieved (p = 0.076), we found higher frequency of variant allele in patients compared to controls (1.87% vs. 0.38%, respectively) which may point to a possible role of this polymorphism in thrombotic events. For the MTHFR677C > T and PAI-1 -675 4G/5G, we found no difference in distributions of genotype or allele frequencies between these two groups (p > 0.05). For three subjects with very rare genotypes (two patients homozygous for FV1691G > A and one patient homozygous for FII20210G > A) we performed additional biochemical analyses for haemostasis, as well as genotyping of two polymorphisms (MTHFR1298A > C and ATIII786G > A).
T2  - Human Fertility
T1  - Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations
DO  - 10.1080/14647273.2016.1255785
SP  - 1
EP  - 8
ER  - 

@misc{
author = "Đurović, Jelena and Stojković, Oliver and Todorović, Jelena and Brajić, Aleksandra and Stanković, Sanja and Obradović, Svetlana and Stamenković, Gorana",
year = "2016",
abstract = "Reproductive failure (recurrent foetal loss, unexplained infertility and IVF implantation failure) may be, in a number of cases, explained by thrombophilia, either acquired or inherited. Several genes contribute to thrombophilia, some with major effect (Factor V, Factor II), and some with minor effect (MTHFR, PAI-1, ATIII, etc.). The aim of this study was to estimate frequency of thrombophilia-associated genotypes (FII20210G > A, FV1691G > A, MTHFR677C > T and PAI-1 -675 4G/5G) in a group of 1631 Serbian women experiencing reproductive failure, and compare it with a healthy, female control group. Our results showed marginally significant (p = 0.050) differences in allele frequencies between patients and controls for the FV1691 mutations. For the FII20210G > A, although the statistical significance was not achieved (p = 0.076), we found higher frequency of variant allele in patients compared to controls (1.87% vs. 0.38%, respectively) which may point to a possible role of this polymorphism in thrombotic events. For the MTHFR677C > T and PAI-1 -675 4G/5G, we found no difference in distributions of genotype or allele frequencies between these two groups (p > 0.05). For three subjects with very rare genotypes (two patients homozygous for FV1691G > A and one patient homozygous for FII20210G > A) we performed additional biochemical analyses for haemostasis, as well as genotyping of two polymorphisms (MTHFR1298A > C and ATIII786G > A).",
journal = "Human Fertility",
title = "Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations",
doi = "10.1080/14647273.2016.1255785",
pages = "1-8"
}

Đurović, J., Stojković, O., Todorović, J., Brajić, A., Stanković, S., Obradović, S.,& Stamenković, G.. (2016). Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations. in Human Fertility, 1-8.
https://doi.org/10.1080/14647273.2016.1255785

Đurović J, Stojković O, Todorović J, Brajić A, Stanković S, Obradović S, Stamenković G. Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations. in Human Fertility. 2016;:1-8.
doi:10.1080/14647273.2016.1255785 .

Đurović, Jelena, Stojković, Oliver, Todorović, Jelena, Brajić, Aleksandra, Stanković, Sanja, Obradović, Svetlana, Stamenković, Gorana, "Genetics of suspected thrombophilia in Serbian females with infertility, including three cases, homozygous for FII 20210A or FV 1691A mutations" in Human Fertility (2016):1-8,
https://doi.org/10.1080/14647273.2016.1255785 . .