TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Dias, Maria Inês
AU  - Zengin, Gokhan
AU  - Barros, Lillian
AU  - Ivanov, Marija
AU  - Gašić, Uroš
AU  - Rajčević, Nemanja
AU  - Stevanović, Milena
AU  - Ferreira, Isabel C.F.R.
AU  - Soković, Marina
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4871
AB  - Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa extract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic effect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.
PB  - Dortmund: EXCLI Journal
T2  - EXCLI Journal
T1  - Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line
VL  - 21
DO  - 10.17179/excli2021-4487
SP  - 387
EP  - 399
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Dias, Maria Inês and Zengin, Gokhan and Barros, Lillian and Ivanov, Marija and Gašić, Uroš and Rajčević, Nemanja and Stevanović, Milena and Ferreira, Isabel C.F.R. and Soković, Marina",
year = "2022",
abstract = "Despite the significant advances in drug development we are witnessing the inability of health systems to combat both neurodegenerative diseases and cancers, especially glioblastoma. Hence, natural products are comprehen-sively studied in order to provide novel therapeutic options. This study aimed to explore anti-neurodegenerative and anti-glioblastoma potential of extract of Phlomis fruticosa L. using in vitro model systems. It was found that the methanol extract of P. fruticosa was able to efficiently reduce activities of enzymes linked to neurodegenera-tive disease including acetylcholinesterase, butyrylcholinesterase and tyrosinase. Furthermore, P. fruticosa extract has shown excellent antioxidant potential, as evidenced by six different methods. Analysis of cytotoxic effect of P. fruticosa extract on A172 glioblastoma cell line revealed that the concentration of the extract necessary for 50 % inhibition of A172 growth (IC50) was 710 μg/mL. The extract did not induce changes in proliferation and morphology of A172 glioblastoma cells. On the other side, production of ROS was increased in A172 cells treated with the extract. Observed cytotoxic effect of P. fruticosa extract might be based on increase in ROS generation upon treatment. Quantitative chemical analysis revealed the presence of twelve different polyphenols with the cis 3-O-caffeoylquinic acid being the most abundant. This study provided scientific evidence for further exploration of P. fruticosa as a promising natural anti-neurodegenerative therapeutic option.",
publisher = "Dortmund: EXCLI Journal",
journal = "EXCLI Journal",
title = "Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line",
volume = "21",
doi = "10.17179/excli2021-4487",
pages = "387-399"
}

Stojković, D., Drakulić, D., Dias, M. I., Zengin, G., Barros, L., Ivanov, M., Gašić, U., Rajčević, N., Stevanović, M., Ferreira, I. C.F.R.,& Soković, M.. (2022). Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line. in EXCLI Journal
Dortmund: EXCLI Journal., 21, 387-399.
https://doi.org/10.17179/excli2021-4487

Stojković D, Drakulić D, Dias MI, Zengin G, Barros L, Ivanov M, Gašić U, Rajčević N, Stevanović M, Ferreira IC, Soković M. Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line. in EXCLI Journal. 2022;21:387-399.
doi:10.17179/excli2021-4487 .

Stojković, Dejan, Drakulić, Danijela, Dias, Maria Inês, Zengin, Gokhan, Barros, Lillian, Ivanov, Marija, Gašić, Uroš, Rajčević, Nemanja, Stevanović, Milena, Ferreira, Isabel C.F.R., Soković, Marina, "Phlomis Fruticosa L. Exerts in Vitro Antineurodegenerative and Antioxidant Activities and Induces Prooxidant Effect in Glioblastoma Cell Line" in EXCLI Journal, 21 (2022):387-399,
https://doi.org/10.17179/excli2021-4487 . .

TY  - JOUR
AU  - Ivanov, Marija
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Drakulić, Danijela
AU  - Stevanović, Milena
AU  - Rajčević, Nemanja
AU  - Stojković, Dejan
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669021010256
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4696
AB  - Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound healing, antioxidant and antidiabetic potentials as well as antimicrobial and antibiofilm capacities; followed by the determination of the antimicrobial mechanism. The extracts yield ranged from 3.03% to 15.75% and they were dominated by epigallocatechin gallate and rosmarinic acid. The majority of extracts did not possess cytotoxic properties towards human keratinocytes. Butanol was the most appropriate solvent for the improvement of wound healing capacity. Combined extraction with ethanol and water resulted in the highest antioxidant potential of the extracts that could be attributed to the high abundance of detected polyphenols. Extraction with butanol led to the increased inhibition towards enzymes linked to type 2-diabetes and increase in antimicrobial capacities. The extracts have shown antibiofilm potential towards Staphylococcus lugdunensis biofilms with more than 90% inhibition for the most active ones. The selected extract EB7 reduced bacterial ability to adhere to human keratinocytes and provided immunomodulatory action in HaCaT cells exposed to S. lugdunensis. The antibacterial mechanism of action of the same extract was associated with moderate perturbation in the cell membrane integrity and with the reduction in bacterial mitochondrial activity. S. nemorosa inflorescences were abundant sources of bioactive polyphenols and with different extraction solvents their bioactivities were further improved. This study reported for the first time the effects of extraction solvents on various bioactivities of S. nemorosa inflorescences, supporting S. nemorosa role as a natural bioactive therapeutic agent with possible antioxidant, antidiabetic and antimicrobial applications.
PB  - Amsterdam: Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents
VL  - 175
DO  - 10.1016/j.indcrop.2021.114260
SP  - 114260
ER  - 

@article{
author = "Ivanov, Marija and Božunović, Jelena and Gašić, Uroš and Drakulić, Danijela and Stevanović, Milena and Rajčević, Nemanja and Stojković, Dejan",
year = "2022",
abstract = "Salvia nemorosa L. is a rich source of bioactive constituents. We have used different solvents to obtain 14 different extracts in order to evaluate solvents impact on S. nemorosa inflorescences bioactivities. Firstly, the extraction yield, chemical composition and cytotoxic properties towards HaCaT cell line were elaborated. Afterwards, we have studied the extracts bioactivity in terms of wound healing, antioxidant and antidiabetic potentials as well as antimicrobial and antibiofilm capacities; followed by the determination of the antimicrobial mechanism. The extracts yield ranged from 3.03% to 15.75% and they were dominated by epigallocatechin gallate and rosmarinic acid. The majority of extracts did not possess cytotoxic properties towards human keratinocytes. Butanol was the most appropriate solvent for the improvement of wound healing capacity. Combined extraction with ethanol and water resulted in the highest antioxidant potential of the extracts that could be attributed to the high abundance of detected polyphenols. Extraction with butanol led to the increased inhibition towards enzymes linked to type 2-diabetes and increase in antimicrobial capacities. The extracts have shown antibiofilm potential towards Staphylococcus lugdunensis biofilms with more than 90% inhibition for the most active ones. The selected extract EB7 reduced bacterial ability to adhere to human keratinocytes and provided immunomodulatory action in HaCaT cells exposed to S. lugdunensis. The antibacterial mechanism of action of the same extract was associated with moderate perturbation in the cell membrane integrity and with the reduction in bacterial mitochondrial activity. S. nemorosa inflorescences were abundant sources of bioactive polyphenols and with different extraction solvents their bioactivities were further improved. This study reported for the first time the effects of extraction solvents on various bioactivities of S. nemorosa inflorescences, supporting S. nemorosa role as a natural bioactive therapeutic agent with possible antioxidant, antidiabetic and antimicrobial applications.",
publisher = "Amsterdam: Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents",
volume = "175",
doi = "10.1016/j.indcrop.2021.114260",
pages = "114260"
}

Ivanov, M., Božunović, J., Gašić, U., Drakulić, D., Stevanović, M., Rajčević, N.,& Stojković, D.. (2022). Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents. in Industrial Crops and Products
Amsterdam: Elsevier B.V.., 175, 114260.
https://doi.org/10.1016/j.indcrop.2021.114260

Ivanov M, Božunović J, Gašić U, Drakulić D, Stevanović M, Rajčević N, Stojković D. Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents. in Industrial Crops and Products. 2022;175:114260.
doi:10.1016/j.indcrop.2021.114260 .

Ivanov, Marija, Božunović, Jelena, Gašić, Uroš, Drakulić, Danijela, Stevanović, Milena, Rajčević, Nemanja, Stojković, Dejan, "Bioactivities of Salvia nemorosa L. inflorescences are influenced by the extraction solvents" in Industrial Crops and Products, 175 (2022):114260,
https://doi.org/10.1016/j.indcrop.2021.114260 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Gašić, Uroš
AU  - Drakulić, Danijela
AU  - Zengin, Gokhan
AU  - Stevanović, Milena
AU  - Rajčević, Nemanja
AU  - Soković, Marina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4229
AB  - Structural diversity of biologically active compounds identified in plants after many years of storage is rarely reported in literature. Herein, we studied chemical profile and biological activities of Phlomis fruticosa L. after plant material storage for 20 years. Chemical analyzes were performed by UHPLC-LTQ-Orbitrap/MS, and revealed presence of 44 compounds: including 13 phenolic acids, 9 phenylethanoids, 20 flavonoids and 2 phenolic related compounds (a phenolic acid derivative and an aliphatic alcohol). The extract showed antimicrobial activity, being the most potent against Aspergillus fumigatus with minimum inhibitory concentration of 0.31 mg/mL. Also, the extract was able to inhibit biofilm formed by Candida species and to inhibit biofilm formation by Staphylococcus aureus. Obtained results revealed that the extract has potential to interfere with the cell membrane permeability of Candida albicans and to suppress production of virulence factor staphyloxanthin in S. aureus. Furthermore, the extract inhibited the activity of α-amylase which is one of the therapeutic targets for diabetes type II. Also, the antiproliferative effect of the extract was demonstrated on human cancer cell lines, while the extract did not exhibit any cytotoxic effect on primary human cells. Based on the obtained results, P. fruticosa could be an interesting source of biologically active compounds even after long term storage.
PB  - Elsevier B.V.
T2  - Journal of Pharmaceutical and Biomedical Analysis
T1  - Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.
VL  - 195
DO  - 10.1016/j.jpba.2020.113884
SP  - 113884
ER  - 

@article{
author = "Stojković, Dejan and Gašić, Uroš and Drakulić, Danijela and Zengin, Gokhan and Stevanović, Milena and Rajčević, Nemanja and Soković, Marina",
year = "2021",
abstract = "Structural diversity of biologically active compounds identified in plants after many years of storage is rarely reported in literature. Herein, we studied chemical profile and biological activities of Phlomis fruticosa L. after plant material storage for 20 years. Chemical analyzes were performed by UHPLC-LTQ-Orbitrap/MS, and revealed presence of 44 compounds: including 13 phenolic acids, 9 phenylethanoids, 20 flavonoids and 2 phenolic related compounds (a phenolic acid derivative and an aliphatic alcohol). The extract showed antimicrobial activity, being the most potent against Aspergillus fumigatus with minimum inhibitory concentration of 0.31 mg/mL. Also, the extract was able to inhibit biofilm formed by Candida species and to inhibit biofilm formation by Staphylococcus aureus. Obtained results revealed that the extract has potential to interfere with the cell membrane permeability of Candida albicans and to suppress production of virulence factor staphyloxanthin in S. aureus. Furthermore, the extract inhibited the activity of α-amylase which is one of the therapeutic targets for diabetes type II. Also, the antiproliferative effect of the extract was demonstrated on human cancer cell lines, while the extract did not exhibit any cytotoxic effect on primary human cells. Based on the obtained results, P. fruticosa could be an interesting source of biologically active compounds even after long term storage.",
publisher = "Elsevier B.V.",
journal = "Journal of Pharmaceutical and Biomedical Analysis",
title = "Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.",
volume = "195",
doi = "10.1016/j.jpba.2020.113884",
pages = "113884"
}

Stojković, D., Gašić, U., Drakulić, D., Zengin, G., Stevanović, M., Rajčević, N.,& Soković, M.. (2021). Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.. in Journal of Pharmaceutical and Biomedical Analysis
Elsevier B.V.., 195, 113884.
https://doi.org/10.1016/j.jpba.2020.113884

Stojković D, Gašić U, Drakulić D, Zengin G, Stevanović M, Rajčević N, Soković M. Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L.. in Journal of Pharmaceutical and Biomedical Analysis. 2021;195:113884.
doi:10.1016/j.jpba.2020.113884 .

Stojković, Dejan, Gašić, Uroš, Drakulić, Danijela, Zengin, Gokhan, Stevanović, Milena, Rajčević, Nemanja, Soković, Marina, "Chemical profiling, antimicrobial, anti-enzymatic, and cytotoxic properties of Phlomis fruticosa L." in Journal of Pharmaceutical and Biomedical Analysis, 195 (2021):113884,
https://doi.org/10.1016/j.jpba.2020.113884 . .

TY  - JOUR
AU  - Veličković, Ivona
AU  - Žižak, Željko
AU  - Rajčević, Nemanja
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Marin, Petar
AU  - Grujić, Slavica
PY  - 2021
UR  - https://www.notulaebotanicae.ro/index.php/nbha/article/view/12137
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4213
AB  - Prunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry.
PB  - University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
T2  - Notulae Botanicae Horti Agrobotanici Cluj-Napoca
T1  - Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities
IS  - 1
VL  - 49
DO  - 10.15835/nbha49112137
SP  - 12137
ER  - 

@article{
author = "Veličković, Ivona and Žižak, Željko and Rajčević, Nemanja and Ivanov, Marija and Soković, Marina and Marin, Petar and Grujić, Slavica",
year = "2021",
abstract = "Prunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry.",
publisher = "University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca",
journal = "Notulae Botanicae Horti Agrobotanici Cluj-Napoca",
title = "Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities",
number = "1",
volume = "49",
doi = "10.15835/nbha49112137",
pages = "12137"
}

Veličković, I., Žižak, Ž., Rajčević, N., Ivanov, M., Soković, M., Marin, P.,& Grujić, S.. (2021). Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca
University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca., 49(1), 12137.
https://doi.org/10.15835/nbha49112137

Veličković I, Žižak Ž, Rajčević N, Ivanov M, Soković M, Marin P, Grujić S. Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities. in Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2021;49(1):12137.
doi:10.15835/nbha49112137 .

Veličković, Ivona, Žižak, Željko, Rajčević, Nemanja, Ivanov, Marija, Soković, Marina, Marin, Petar, Grujić, Slavica, "Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities" in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 49, no. 1 (2021):12137,
https://doi.org/10.15835/nbha49112137 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Schwirtlich, Marija
AU  - Rajčević, Nemanja
AU  - Stevanović, Milena
AU  - Soković, Marina
AU  - Gašić, Uroš
PY  - 2021
UR  - https://www.mdpi.com/1424-8247/14/1/55
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4209
AB  - Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.
PB  - MDPI AG
T2  - Pharmaceuticals
T1  - Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions
IS  - 1
VL  - 14
DO  - 10.3390/ph14010055
SP  - 55
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Schwirtlich, Marija and Rajčević, Nemanja and Stevanović, Milena and Soković, Marina and Gašić, Uroš",
year = "2021",
abstract = "Anthriscus cerefolium (L.) Hoffm. is a plant traditionally used around the globe since antiquity. Although widely used in many traditional medicines in different cultures, from the scientific point of view it is poorly investigated. Glioblastoma, a tumor type with poor prognosis, is the most common and lethal brain tumor in adults. Current therapeutic strategies for glioblastoma include surgery, radiation and chemotherapy. On the other hand, it has been revealed that patients with cancers are highly susceptible to microbial infections due to the invasive nature of cancer treatment approaches. This study was designed to investigate the chemical profile of herba Anthriscii cerefoli methanolic extract by applying UHPLC-LTQ OrbiTrap MS4 analysis and to analyze its anti-glioblastoma and antimicrobial activities. This study revealed that methanolic extract of herba Anthrisc cerefolii contained phenolic acids and flavonoids, with 32 compounds being identified. Anti-glioblastoma activity was investigated in vitro using A172 glioblastoma cell line. The cytotoxic effects of the extract on A172 cells were compared to the same effect on primary human gingival fibroblast (HGF-1) cells. Decreased rate of proliferation and changes in cell morphology were detected upon treatment of A172 cells with the extract. The antimicrobial activity of extract was tested against Staphylococcus aureus and Candida species. The extract was active against the tested bacterium and yeasts, inhibiting free floating cells and microbial biofilms. This study is the first one to provide a detailed description of the chemical profile of A. cerefolium extract dealing with scientific insights into its anti-glioblastoma and antimicrobial activities.",
publisher = "MDPI AG",
journal = "Pharmaceuticals",
title = "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions",
number = "1",
volume = "14",
doi = "10.3390/ph14010055",
pages = "55"
}

Stojković, D., Drakulić, D., Schwirtlich, M., Rajčević, N., Stevanović, M., Soković, M.,& Gašić, U.. (2021). Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals
MDPI AG., 14(1), 55.
https://doi.org/10.3390/ph14010055

Stojković D, Drakulić D, Schwirtlich M, Rajčević N, Stevanović M, Soković M, Gašić U. Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions. in Pharmaceuticals. 2021;14(1):55.
doi:10.3390/ph14010055 .

Stojković, Dejan, Drakulić, Danijela, Schwirtlich, Marija, Rajčević, Nemanja, Stevanović, Milena, Soković, Marina, Gašić, Uroš, "Extract of Herba Anthrisci cerefolii: Chemical Profiling and Insights into Its Anti-Glioblastoma and Antimicrobial Mechanism of Actions" in Pharmaceuticals, 14, no. 1 (2021):55,
https://doi.org/10.3390/ph14010055 . .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Drakulić, Danijela
AU  - Gašić, Uroš
AU  - Zengin, Gokhan
AU  - Stevanović, Milena
AU  - Rajčević, Nemanja
AU  - Soković, Marina
PY  - 2020
UR  - https://pubs.rsc.org/en/content/articlehtml/2020/fo/d0fo01595d
UR  - https://pubs.rsc.org/en/content/articlelanding/2020/fo/d0fo01595d
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32749435
UR  - https://radar.ibiss.bg.ac.rs/123456789/3853
AB  - This study explored the chemical profile of the aerial parts of Ononis spinosa and further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential against Staphylococcus aureus and was able to supress the production of staphyloxanthin in S. aureus at sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.
PB  - Royal Society of Chemistry (RSC)
T2  - Food & Function
T1  - Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract
IS  - 8
VL  - 11
DO  - 10.1039/d0fo01595d
SP  - 7138
EP  - 7151
ER  - 

@article{
author = "Stojković, Dejan and Drakulić, Danijela and Gašić, Uroš and Zengin, Gokhan and Stevanović, Milena and Rajčević, Nemanja and Soković, Marina",
year = "2020",
abstract = "This study explored the chemical profile of the aerial parts of Ononis spinosa and further investigated its biological activities. Chemical profiling of the extract revealed the presence of 63 different compounds: phenolic acids, flavonoid glycosides and aglycones, isoflavonoid glycosides and aglycones, and other related compounds. Our results revealed that the extract was active against 8 strains of free floating bacteria. It showed anti-biofilm potential against Staphylococcus aureus and was able to supress the production of staphyloxanthin in S. aureus at sub-minimal inhibitory concentrations. Its antioxidant activity was evaluated by using several assays (phosphomolybdenum, DPPH, ABTS, CUPRAC, FRAP, and metal chelating assay), which showed that the extract exhibited a dose dependent activity. Inhibition of AChE, BChE, amylase, glucosidase and tyrosinase was achieved by the extract, demonstrating its anti-enzymatic activity. The antiproliferative potential of the extract towards human cancer cell lines (HepG2, MCF-7, SiHa and A172) was determined by using the crystal violet assay. Ki67, a marker of proliferation was downregulated in the A172 glioblastoma cell line.",
publisher = "Royal Society of Chemistry (RSC)",
journal = "Food & Function",
title = "Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract",
number = "8",
volume = "11",
doi = "10.1039/d0fo01595d",
pages = "7138-7151"
}

Stojković, D., Drakulić, D., Gašić, U., Zengin, G., Stevanović, M., Rajčević, N.,& Soković, M.. (2020). Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function
Royal Society of Chemistry (RSC)., 11(8), 7138-7151.
https://doi.org/10.1039/d0fo01595d

Stojković D, Drakulić D, Gašić U, Zengin G, Stevanović M, Rajčević N, Soković M. Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract. in Food & Function. 2020;11(8):7138-7151.
doi:10.1039/d0fo01595d .

Stojković, Dejan, Drakulić, Danijela, Gašić, Uroš, Zengin, Gokhan, Stevanović, Milena, Rajčević, Nemanja, Soković, Marina, "Ononis spinosa L., an edible and medicinal plant: UHPLC-LTQ-Orbitrap/MS chemical profiling and biological activities of the herbal extract" in Food & Function, 11, no. 8 (2020):7138-7151,
https://doi.org/10.1039/d0fo01595d . .

TY  - JOUR
AU  - Veličković, Ivona
AU  - Žižak, Željko
AU  - Rajčević, Nemanja
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Marin, Petar
AU  - Grujić, Slavica
PY  - 2020
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4932
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3652
AB  - We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson’s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of α-amylase and α-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent α-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further.
T2  - Archives of Biological Sciences
T1  - Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts
IS  - 1
VL  - 72
DO  - 10.2298/abs191217004v
SP  - 105
EP  - 115
ER  - 

@article{
author = "Veličković, Ivona and Žižak, Željko and Rajčević, Nemanja and Ivanov, Marija and Soković, Marina and Marin, Petar and Grujić, Slavica",
year = "2020",
abstract = "We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson’s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of α-amylase and α-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent α-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further.",
journal = "Archives of Biological Sciences",
title = "Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts",
number = "1",
volume = "72",
doi = "10.2298/abs191217004v",
pages = "105-115"
}

Veličković, I., Žižak, Ž., Rajčević, N., Ivanov, M., Soković, M., Marin, P.,& Grujić, S.. (2020). Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts. in Archives of Biological Sciences, 72(1), 105-115.
https://doi.org/10.2298/abs191217004v

Veličković I, Žižak Ž, Rajčević N, Ivanov M, Soković M, Marin P, Grujić S. Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts. in Archives of Biological Sciences. 2020;72(1):105-115.
doi:10.2298/abs191217004v .

Veličković, Ivona, Žižak, Željko, Rajčević, Nemanja, Ivanov, Marija, Soković, Marina, Marin, Petar, Grujić, Slavica, "Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts" in Archives of Biological Sciences, 72, no. 1 (2020):105-115,
https://doi.org/10.2298/abs191217004v . .

TY  - JOUR
AU  - Nikolić, Biljana
AU  - Ljujić, Jovana
AU  - Bojović, Srđan
AU  - Mitić, Zorica
AU  - Rajčević, Nemanja
AU  - Tešević, Vele
AU  - Marin, Petar
PY  - 2020
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642000038N
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4040
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/5348
AB  - The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were ?-pinene (29.5 %), ?-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, ?-phellandrene, ?-3-carene, p-cymene, ?-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (?-pinene, ?-pinene, myrcene, ?-3-carene, ?-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.
PB  - Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Headspace volatiles isolated from twigs of Picea omorika from Serbia
IS  - 3
VL  - 72
DO  - 10.2298/ABS200511038N
SP  - 445
EP  - 452
ER  - 

@article{
author = "Nikolić, Biljana and Ljujić, Jovana and Bojović, Srđan and Mitić, Zorica and Rajčević, Nemanja and Tešević, Vele and Marin, Petar",
year = "2020",
abstract = "The variability of volatiles isolated from twigs by the static headspace (HS) method in seven natural populations of Picea omorika from Serbia was investigated for the first time. In the overall chemical profile, monoterpenes strongly dominated hydrocarbons as the most volatile compounds (95.7%). The dominant compounds were ?-pinene (29.5 %), ?-pinene (25.7%) and myrcene (13.0%), totaling 68.2% of the volatiles on average. The following nine volatiles were found to be present in medium-to-high amounts (0.5-10%): tricyclene, camphene, ?-phellandrene, ?-3-carene, p-cymene, ?-phellandrene, terpinolene, (E)-caryophyllene, and germacrene D. Out of the 78 volatiles detected, the six most abundant (?-pinene, ?-pinene, myrcene, ?-3-carene, ?-phellandrene and camphene) were selected for principle component analysis (PCA) and cluster analyses (CA). PCA revealed a high degree of similarity between populations, while CA showed a degree of separation of two populations from the others. The presented results are in agreement with previous phytochemical and molecular analyses of this species that confirm high variability in both specialized metabolites and genetic markers.",
publisher = "Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Headspace volatiles isolated from twigs of Picea omorika from Serbia",
number = "3",
volume = "72",
doi = "10.2298/ABS200511038N",
pages = "445-452"
}

Nikolić, B., Ljujić, J., Bojović, S., Mitić, Z., Rajčević, N., Tešević, V.,& Marin, P.. (2020). Headspace volatiles isolated from twigs of Picea omorika from Serbia. in Archives of Biological Sciences
Serbian Biological Society., 72(3), 445-452.
https://doi.org/10.2298/ABS200511038N

Nikolić B, Ljujić J, Bojović S, Mitić Z, Rajčević N, Tešević V, Marin P. Headspace volatiles isolated from twigs of Picea omorika from Serbia. in Archives of Biological Sciences. 2020;72(3):445-452.
doi:10.2298/ABS200511038N .

Nikolić, Biljana, Ljujić, Jovana, Bojović, Srđan, Mitić, Zorica, Rajčević, Nemanja, Tešević, Vele, Marin, Petar, "Headspace volatiles isolated from twigs of Picea omorika from Serbia" in Archives of Biological Sciences, 72, no. 3 (2020):445-452,
https://doi.org/10.2298/ABS200511038N . .

TY  - JOUR
AU  - Kovačević, Dragan
AU  - Nikolić, Biljana
AU  - Mladenović-Drinić, Snežana
AU  - Bojović, Srđan
AU  - Dodoš, Tanja
AU  - Rajčević, Nemanja
AU  - Marin, Petar D.
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/449
AB  - Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus.
AB  - Prikazana su proučavanja sa ciljem utvrđivanja genetičke veze 10 različitih vrsta familije Pinaceae putem nasumično umnoženih polimorfnih DNA (RAPD) markera. Osamnaest proizvoljnih RAPD prajmera produkovalo je 123 fragmenata od kojih 107 polimorfnih (87%). Vrednosti koeficijenta sličnosti su varirale od 0.34 do 0.67. Najviši koeficijent sličnosti je detektovan između Pinus wallichiana i P. strobus, kao i između Picea abies i P. orientalis,a najniži između tri vrste roda Pinus (P. heldreichii,P. peuce i P. wallichiana)i Picea omorika. Analiza RAPD markera potvrdila je genetičku vezu između vrsta. Rod Picea se jasno razdvojio od roda Pinus i bliži je rodu Abies (A. concolor) nego rodu Pinus, što potvrđuje dosadašnje brojne uporedno-morfološke, anatomske, hemotaksonomske i molekularne rezultate ovih blisko srodnih rodova. Nadalje, na osnovu naših rezultata, jasno se razdvajaju analizirane vrste borova iz različitih podrodova-Pinus i Strobus, što je, takođe, u saglasnosti sa savremenom intrageneričkom klasifikacijom roda Pinus.
T2  - Genetika
T1  - Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera
T1  - Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers
IS  - 2
VL  - 45
SP  - 493
EP  - 502
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_449
ER  - 

@article{
author = "Kovačević, Dragan and Nikolić, Biljana and Mladenović-Drinić, Snežana and Bojović, Srđan and Dodoš, Tanja and Rajčević, Nemanja and Marin, Petar D.",
year = "2013, 2013",
abstract = "Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus., Prikazana su proučavanja sa ciljem utvrđivanja genetičke veze 10 različitih vrsta familije Pinaceae putem nasumično umnoženih polimorfnih DNA (RAPD) markera. Osamnaest proizvoljnih RAPD prajmera produkovalo je 123 fragmenata od kojih 107 polimorfnih (87%). Vrednosti koeficijenta sličnosti su varirale od 0.34 do 0.67. Najviši koeficijent sličnosti je detektovan između Pinus wallichiana i P. strobus, kao i između Picea abies i P. orientalis,a najniži između tri vrste roda Pinus (P. heldreichii,P. peuce i P. wallichiana)i Picea omorika. Analiza RAPD markera potvrdila je genetičku vezu između vrsta. Rod Picea se jasno razdvojio od roda Pinus i bliži je rodu Abies (A. concolor) nego rodu Pinus, što potvrđuje dosadašnje brojne uporedno-morfološke, anatomske, hemotaksonomske i molekularne rezultate ovih blisko srodnih rodova. Nadalje, na osnovu naših rezultata, jasno se razdvajaju analizirane vrste borova iz različitih podrodova-Pinus i Strobus, što je, takođe, u saglasnosti sa savremenom intrageneričkom klasifikacijom roda Pinus.",
journal = "Genetika",
title = "Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera, Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers",
number = "2",
volume = "45",
pages = "493-502",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_449"
}

Kovačević, D., Nikolić, B., Mladenović-Drinić, S., Bojović, S., Dodoš, T., Rajčević, N.,& Marin, P. D.. (2013). Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera. in Genetika, 45(2), 493-502.
https://hdl.handle.net/21.15107/rcub_ibiss_449

Kovačević D, Nikolić B, Mladenović-Drinić S, Bojović S, Dodoš T, Rajčević N, Marin PD. Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera. in Genetika. 2013;45(2):493-502.
https://hdl.handle.net/21.15107/rcub_ibiss_449 .

Kovačević, Dragan, Nikolić, Biljana, Mladenović-Drinić, Snežana, Bojović, Srđan, Dodoš, Tanja, Rajčević, Nemanja, Marin, Petar D., "Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera" in Genetika, 45, no. 2 (2013):493-502,
https://hdl.handle.net/21.15107/rcub_ibiss_449 .