TY  - JOUR
AU  - Lazarević, Jelica
AU  - Milanović, Slobodan
AU  - Šešlija Jovanović, Darka
AU  - Janković Tomanić, Milena
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5721
AB  - Temperature and food quality are the most important environmental factors determining
the performance of herbivorous insects. The objective of our study was to evaluate the responses of
the spongy moth (formerly known as the gypsy moth) [Lymantria dispar L. (Lepidoptera: Erebidae)] to
simultaneous variation in these two factors. From hatching to the fourth instar, larvae were exposed
to three temperatures (19  C, 23  C, and 28  C) and fed four artificial diets that differed in protein (P)
and carbohydrate (C) content. Within each temperature regime, the effects of the nutrient content
(P+C) and ratio (P:C) on development duration, larval mass, growth rate, and activities of digestive
proteases, carbohydrases, and lipase were examined. It was found that temperature and food quality
had a significant effect on the fitness-related traits and digestive physiology of the larvae. The greatest
mass and highest growth rate were obtained at 28  C on a high-protein low-carbohydrate diet. A
homeostatic increase in activity was observed for total protease, trypsin, and amylase in response to
low substrate levels in the diet. A significant modulation of overall enzyme activities in response
to 28  C was detected only with a low diet quality. A decrease in the nutrient content and P:C ratio
only affected the coordination of enzyme activities at 28  C, as indicated by the significantly altered
correlation matrices. Multiple linear regression analysis showed that variation in fitness traits in
response to different rearing conditions could be explained by variation in digestion. Our results
contribute to the understanding of the role of digestive enzymes in post-ingestive nutrient balancing
PB  - Basel: MDPI
T2  - Biomolecules
T1  - Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae
IS  - 5
VL  - 13
DO  - 10.3390/biom13050821
SP  - 821
ER  - 

@article{
author = "Lazarević, Jelica and Milanović, Slobodan and Šešlija Jovanović, Darka and Janković Tomanić, Milena",
year = "2023",
abstract = "Temperature and food quality are the most important environmental factors determining
the performance of herbivorous insects. The objective of our study was to evaluate the responses of
the spongy moth (formerly known as the gypsy moth) [Lymantria dispar L. (Lepidoptera: Erebidae)] to
simultaneous variation in these two factors. From hatching to the fourth instar, larvae were exposed
to three temperatures (19  C, 23  C, and 28  C) and fed four artificial diets that differed in protein (P)
and carbohydrate (C) content. Within each temperature regime, the effects of the nutrient content
(P+C) and ratio (P:C) on development duration, larval mass, growth rate, and activities of digestive
proteases, carbohydrases, and lipase were examined. It was found that temperature and food quality
had a significant effect on the fitness-related traits and digestive physiology of the larvae. The greatest
mass and highest growth rate were obtained at 28  C on a high-protein low-carbohydrate diet. A
homeostatic increase in activity was observed for total protease, trypsin, and amylase in response to
low substrate levels in the diet. A significant modulation of overall enzyme activities in response
to 28  C was detected only with a low diet quality. A decrease in the nutrient content and P:C ratio
only affected the coordination of enzyme activities at 28  C, as indicated by the significantly altered
correlation matrices. Multiple linear regression analysis showed that variation in fitness traits in
response to different rearing conditions could be explained by variation in digestion. Our results
contribute to the understanding of the role of digestive enzymes in post-ingestive nutrient balancing",
publisher = "Basel: MDPI",
journal = "Biomolecules",
title = "Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae",
number = "5",
volume = "13",
doi = "10.3390/biom13050821",
pages = "821"
}

Lazarević, J., Milanović, S., Šešlija Jovanović, D.,& Janković Tomanić, M.. (2023). Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae. in Biomolecules
Basel: MDPI., 13(5), 821.
https://doi.org/10.3390/biom13050821

Lazarević J, Milanović S, Šešlija Jovanović D, Janković Tomanić M. Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae. in Biomolecules. 2023;13(5):821.
doi:10.3390/biom13050821 .

Lazarević, Jelica, Milanović, Slobodan, Šešlija Jovanović, Darka, Janković Tomanić, Milena, "Temperature- and Diet-Induced Plasticity of Growth and Digestive Enzymes Activity in Spongy Moth Larvae" in Biomolecules, 13, no. 5 (2023):821,
https://doi.org/10.3390/biom13050821 . .

TY  - JOUR
AU  - Popović, Vladan
AU  - Šešlija Jovanović, Darka
AU  - Miletić, Zoran
AU  - Milovanović, Jelena
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Miljković, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5232
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5248
AB  - The aim of this research was to quantifythe content of hazardous elements in the needles ofNorway spruce (Picea abies L.) in the natural habitatsthat were accumulated from thermal power plants,mines, and metal processing industry. Fifteen naturalpopulations of the Norway spruce were sampled fromthe mountain ranges in Southeastern Europe (DinaricAlps and Balkan Mountains). Two-year-old spruceneedles were evaluated the content of the followinghazardous elements: heavy metals cadmium, mercury,nickel, lead and zinc, and metalloid arsenic. The effectof the distance between air pollution emitters and theNorway spruce natural habitats on the hazardous elementscontent in needles was also evaluated. Theresults of the analysis of variance confirmed interpopulationdifferences in the content of all analyzed hazardous elements. The effect of the air pollutionsource (thermal power plants, mines, and industry) onthe content of hazardous elements in the spruce needleswas also assessed. Significant correlation wasfound between the distance of air pollution emittersand the amount of zinc. This study could serve as thestartup point of future monitoring programs and providenew prospect of using Norway spruce needlesas the bioindicator of air pollution with hazardouselements on Balkan Peninsula since the fact that theNorway spruce natural populations inhabit wide geographicrange of the continental Europe, from the BalkanPeninsula, over European Alps to Scandinavia anda large-scale of altitude from 980 to 1860 m above sealevel.
PB  - Springer Nature
T2  - Environmental Monitoring and Assessment
T1  - The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats
IS  - 109
VL  - 195
DO  - 10.1007/s10661-022-10732-2
ER  - 

@article{
author = "Popović, Vladan and Šešlija Jovanović, Darka and Miletić, Zoran and Milovanović, Jelena and Lučić, Aleksandar and Rakonjac, Ljubinko and Miljković, Danijela",
year = "2023",
abstract = "The aim of this research was to quantifythe content of hazardous elements in the needles ofNorway spruce (Picea abies L.) in the natural habitatsthat were accumulated from thermal power plants,mines, and metal processing industry. Fifteen naturalpopulations of the Norway spruce were sampled fromthe mountain ranges in Southeastern Europe (DinaricAlps and Balkan Mountains). Two-year-old spruceneedles were evaluated the content of the followinghazardous elements: heavy metals cadmium, mercury,nickel, lead and zinc, and metalloid arsenic. The effectof the distance between air pollution emitters and theNorway spruce natural habitats on the hazardous elementscontent in needles was also evaluated. Theresults of the analysis of variance confirmed interpopulationdifferences in the content of all analyzed hazardous elements. The effect of the air pollutionsource (thermal power plants, mines, and industry) onthe content of hazardous elements in the spruce needleswas also assessed. Significant correlation wasfound between the distance of air pollution emittersand the amount of zinc. This study could serve as thestartup point of future monitoring programs and providenew prospect of using Norway spruce needlesas the bioindicator of air pollution with hazardouselements on Balkan Peninsula since the fact that theNorway spruce natural populations inhabit wide geographicrange of the continental Europe, from the BalkanPeninsula, over European Alps to Scandinavia anda large-scale of altitude from 980 to 1860 m above sealevel.",
publisher = "Springer Nature",
journal = "Environmental Monitoring and Assessment",
title = "The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats",
number = "109",
volume = "195",
doi = "10.1007/s10661-022-10732-2"
}

Popović, V., Šešlija Jovanović, D., Miletić, Z., Milovanović, J., Lučić, A., Rakonjac, L.,& Miljković, D.. (2023). The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats. in Environmental Monitoring and Assessment
Springer Nature., 195(109).
https://doi.org/10.1007/s10661-022-10732-2

Popović V, Šešlija Jovanović D, Miletić Z, Milovanović J, Lučić A, Rakonjac L, Miljković D. The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats. in Environmental Monitoring and Assessment. 2023;195(109).
doi:10.1007/s10661-022-10732-2 .

Popović, Vladan, Šešlija Jovanović, Darka, Miletić, Zoran, Milovanović, Jelena, Lučić, Aleksandar, Rakonjac, Ljubinko, Miljković, Danijela, "The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats" in Environmental Monitoring and Assessment, 195, no. 109 (2023),
https://doi.org/10.1007/s10661-022-10732-2 . .

TY  - JOUR
AU  - Popović, Vladan
AU  - Šešlija Jovanović, Darka
AU  - Miletić, Zoran
AU  - Milovanović, Jelena
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Miljković, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5232
AB  - The aim of this research was to quantify
the content of hazardous elements in the needles of
Norway spruce (Picea abies L.) in the natural habitats
that were accumulated from thermal power plants,
mines, and metal processing industry. Fifteen natural
populations of the Norway spruce were sampled from
the mountain ranges in Southeastern Europe (Dinaric
Alps and Balkan Mountains). Two-year-old spruce
needles were evaluated the content of the following
hazardous elements: heavy metals cadmium, mercury,
nickel, lead and zinc, and metalloid arsenic. The effect
of the distance between air pollution emitters and the
Norway spruce natural habitats on the hazardous elements
content in needles was also evaluated. The
results of the analysis of variance confirmed interpopulation
differences in the content of all analyzed hazardous elements. The effect of the air pollution
source (thermal power plants, mines, and industry) on
the content of hazardous elements in the spruce needles
was also assessed. Significant correlation was
found between the distance of air pollution emitters
and the amount of zinc. This study could serve as the
startup point of future monitoring programs and provide
new prospect of using Norway spruce needles
as the bioindicator of air pollution with hazardous
elements on Balkan Peninsula since the fact that the
Norway spruce natural populations inhabit wide geographic
range of the continental Europe, from the Balkan
Peninsula, over European Alps to Scandinavia and
a large-scale of altitude from 980 to 1860 m above sea
level.
PB  - Springer Nature
T2  - Environmental Monitoring and Assessment
T1  - The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats
IS  - 109
VL  - 195
DO  - 10.1007/s10661-022-10732-2
ER  - 

@article{
author = "Popović, Vladan and Šešlija Jovanović, Darka and Miletić, Zoran and Milovanović, Jelena and Lučić, Aleksandar and Rakonjac, Ljubinko and Miljković, Danijela",
year = "2023",
abstract = "The aim of this research was to quantify
the content of hazardous elements in the needles of
Norway spruce (Picea abies L.) in the natural habitats
that were accumulated from thermal power plants,
mines, and metal processing industry. Fifteen natural
populations of the Norway spruce were sampled from
the mountain ranges in Southeastern Europe (Dinaric
Alps and Balkan Mountains). Two-year-old spruce
needles were evaluated the content of the following
hazardous elements: heavy metals cadmium, mercury,
nickel, lead and zinc, and metalloid arsenic. The effect
of the distance between air pollution emitters and the
Norway spruce natural habitats on the hazardous elements
content in needles was also evaluated. The
results of the analysis of variance confirmed interpopulation
differences in the content of all analyzed hazardous elements. The effect of the air pollution
source (thermal power plants, mines, and industry) on
the content of hazardous elements in the spruce needles
was also assessed. Significant correlation was
found between the distance of air pollution emitters
and the amount of zinc. This study could serve as the
startup point of future monitoring programs and provide
new prospect of using Norway spruce needles
as the bioindicator of air pollution with hazardous
elements on Balkan Peninsula since the fact that the
Norway spruce natural populations inhabit wide geographic
range of the continental Europe, from the Balkan
Peninsula, over European Alps to Scandinavia and
a large-scale of altitude from 980 to 1860 m above sea
level.",
publisher = "Springer Nature",
journal = "Environmental Monitoring and Assessment",
title = "The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats",
number = "109",
volume = "195",
doi = "10.1007/s10661-022-10732-2"
}

Popović, V., Šešlija Jovanović, D., Miletić, Z., Milovanović, J., Lučić, A., Rakonjac, L.,& Miljković, D.. (2023). The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats. in Environmental Monitoring and Assessment
Springer Nature., 195(109).
https://doi.org/10.1007/s10661-022-10732-2

Popović V, Šešlija Jovanović D, Miletić Z, Milovanović J, Lučić A, Rakonjac L, Miljković D. The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats. in Environmental Monitoring and Assessment. 2023;195(109).
doi:10.1007/s10661-022-10732-2 .

Popović, Vladan, Šešlija Jovanović, Darka, Miletić, Zoran, Milovanović, Jelena, Lučić, Aleksandar, Rakonjac, Ljubinko, Miljković, Danijela, "The evaluation of hazardous element content in the needles of the Norway spruce (Picea abies L.) that originated from anthropogenic activities in the vicinity of the native habitats" in Environmental Monitoring and Assessment, 195, no. 109 (2023),
https://doi.org/10.1007/s10661-022-10732-2 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Jankovský, Libor
AU  - Lazarević, Jelica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5299
AB  - Botanicals, such as essential oils (EO) and their compounds, are considered a viable ecofriendly
alternative to synthetic insecticides, which threaten human health and ecosystem functioning.
In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid)
and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious
pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology
were assessed at different compound concentrations and compared with the effects of the commercial
botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed
by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice
assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations
of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing
molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth
regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and
food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food
processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results
suggest that anethole and carvone might be used as control agents against GML.
PB  - Basel: MDPI
T2  - Agronomy
T1  - Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)
IS  - 12
VL  - 12
DO  - 10.3390/agronomy12123049
SP  - 3049
ER  - 

@article{
author = "Kostić, Igor and Milanović, Slobodan and Kostić, Miroslav and Šešlija Jovanović, Darka and Ćalić, Dušica and Jankovský, Libor and Lazarević, Jelica",
year = "2022",
abstract = "Botanicals, such as essential oils (EO) and their compounds, are considered a viable ecofriendly
alternative to synthetic insecticides, which threaten human health and ecosystem functioning.
In the present study, we explored the potential use of two EO compounds, trans-anethole (phenylpropanoid)
and S-(+)-carvone (monoterpene ketone), against gypsy moth larvae (GML), a serious
pest of deciduous forests and orchards. GML feeding, survival, molting, and nutritional physiology
were assessed at different compound concentrations and compared with the effects of the commercial
botanical product NeemAzal®-T/S (neem). The impact of botanicals on GML feeding was assessed
by the leaf-dipping method and showed the highest antifeeding activity of neem in the no-choice
assay. GML that were offered a choice were deterred by anethole and attracted by low concentrations
of carvone and neem. Ingestion of botanicals was more effective in inducing mortality and reducing
molting than residual contact exposure. Anethole and carvone were better toxicants but worse growth
regulators than neem. Assessing nutritional indices revealed reduced growth, consumption, and
food utilization in larvae fed on botanical-supplemented diets. The highest metabolic cost of food
processing was recorded in carvone-fed larvae, which exhibited a negative growth rate. The results
suggest that anethole and carvone might be used as control agents against GML.",
publisher = "Basel: MDPI",
journal = "Agronomy",
title = "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)",
number = "12",
volume = "12",
doi = "10.3390/agronomy12123049",
pages = "3049"
}

Kostić, I., Milanović, S., Kostić, M., Šešlija Jovanović, D., Ćalić, D., Jankovský, L.,& Lazarević, J.. (2022). Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy
Basel: MDPI., 12(12), 3049.
https://doi.org/10.3390/agronomy12123049

Kostić I, Milanović S, Kostić M, Šešlija Jovanović D, Ćalić D, Jankovský L, Lazarević J. Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.). in Agronomy. 2022;12(12):3049.
doi:10.3390/agronomy12123049 .

Kostić, Igor, Milanović, Slobodan, Kostić, Miroslav, Šešlija Jovanović, Darka, Ćalić, Dušica, Jankovský, Libor, Lazarević, Jelica, "Antifeeding, Toxic, and Growth-Reducing Activity of trans-Anethole and S-(+)-Carvone against Larvae of the Gypsy Moth Lymantria dispar (L.)" in Agronomy, 12, no. 12 (2022):3049,
https://doi.org/10.3390/agronomy12123049 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Šešlija Jovanović, Darka
AU  - Ćalić, Dušica
AU  - Milanović, Slobodan
AU  - Kostić, Miroslav
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5306
AB  - The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae
species. The use of essential oil compounds in pest management has been proposed as an alternative
to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined
the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and
a thujone‐camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according
to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of
potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased
at higher concentrations of the examined compounds. Camphor was more effective against larvae
and the thujone‐camphor mixture was more effective against adults. Additionally, adults moved
faster towards the control leaf disc in the two‐choice olfactometer assay if an alternative disc was
treated with a thujone‐camphor mixture, whereas larvae responded similarly to the two potential
repellents. However, after contact with the leaf disc treated with the highest compound concentration,
the larvae escaped faster from the thujone‐camphor mixture than from pure camphor. In conclusion,
both examined compounds are promising eco‐friendly antifeedants, but their efficacy depends
on the developmental stage of the beetle, compound type and applied concentration.
PB  - Basel: MDPI
T2  - Plants
T1  - Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle
IS  - 24
VL  - 11
DO  - 10.3390/plants11243587
SP  - 3587
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Šešlija Jovanović, Darka and Ćalić, Dušica and Milanović, Slobodan and Kostić, Miroslav",
year = "2022",
abstract = "The Colorado potato beetle (CPB) is a serious pest of economically important Solanaceae
species. The use of essential oil compounds in pest management has been proposed as an alternative
to harmful chemical insecticides that disturb human health and ecosystem functioning. We examined
the antifeedant activity of three concentrations (0.125%, 0.25% and 0.5%) of pure camphor and
a thujone‐camphor mixture against 3rd instar larvae and adults. Their efficacy was evaluated according
to the degree of leaf damage and avoidance of treated leaves by the CPB. Treatment of
potato leaves significantly reduced leaf damage compared to the control. Leaf protection increased
at higher concentrations of the examined compounds. Camphor was more effective against larvae
and the thujone‐camphor mixture was more effective against adults. Additionally, adults moved
faster towards the control leaf disc in the two‐choice olfactometer assay if an alternative disc was
treated with a thujone‐camphor mixture, whereas larvae responded similarly to the two potential
repellents. However, after contact with the leaf disc treated with the highest compound concentration,
the larvae escaped faster from the thujone‐camphor mixture than from pure camphor. In conclusion,
both examined compounds are promising eco‐friendly antifeedants, but their efficacy depends
on the developmental stage of the beetle, compound type and applied concentration.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle",
number = "24",
volume = "11",
doi = "10.3390/plants11243587",
pages = "3587"
}

Lazarević, J., Kostić, I., Šešlija Jovanović, D., Ćalić, D., Milanović, S.,& Kostić, M.. (2022). Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants
Basel: MDPI., 11(24), 3587.
https://doi.org/10.3390/plants11243587

Lazarević J, Kostić I, Šešlija Jovanović D, Ćalić D, Milanović S, Kostić M. Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle. in Plants. 2022;11(24):3587.
doi:10.3390/plants11243587 .

Lazarević, Jelica, Kostić, Igor, Šešlija Jovanović, Darka, Ćalić, Dušica, Milanović, Slobodan, Kostić, Miroslav, "Pure Camphor and a Thujone‐Camphor Mixture as Eco‐Friendly Antifeedants against Larvae and Adults of the Colorado Potato Beetle" in Plants, 11, no. 24 (2022):3587,
https://doi.org/10.3390/plants11243587 . .

TY  - JOUR
AU  - Popović, Vladan
AU  - Šešlija Jovanović, Darka
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Jovanović, Sanja
AU  - Vasiljević, Aleksandar
AU  - Miljković, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5131
AB  - Interpopulation and intrapopulation variability of three morphological needle traits (length, width and thickness) was investigated in 16 natural silver fir populations in the Balkan Peninsula. The populations represent refugial areas of silver fir (Abies alba Mill.). This paper aims to provide a comprehensive analysis of the influence of climatic factors (mean annual temperature, number of days with temperatures < 0, > 5, < 18, > 18oC, Hargreaves climatic moisture deficit and De Martonne aridity index, on the pattern of morphological needle traits within each population. Populations showed variation in the analyzed morphological needle traits, which could not be clearly defined by any of the analyzed climatic factors. The De Martonne aridity index and Hargreaves climatic moisture deficit had the greatest impact on the trait values, whereas the mean annual precipitation had the lowest. Evolutionary ecology research of the silver fir needle morphology is a valuable contribution to the comprehention of the present genetic variability as a prerequisite for adaptation to the rapid climate change and conservation of the species area in the Balkan Peninsula region.
AB  - Interpopulacijska i intrapopulacijska varijabilnost triju morfoloških osobina iglica (dužina, širina i
debljina) istraživana je u 16 prirodnih populacija jele na Balkanskom poluotoku. Populacije predstav-
ljaju refugijalna područja jele (Abies alba Mill.). Ovaj rad ima za cilj pružiti opsežnu analizu utjecaja
klimatskih čimbenika (srednja godišnja temperatura, broj dana s temperaturama < 0, > 5, < 18, > 18°C,
Hargreavesov klimatski deficit vlage i De Martonneov indeks aridnosti) na obrazac morfoloških
obilježja iglica unutar svake populacije. Populacije su pokazale varijacije u analiziranim morfološkim
obilježjima iglica koje se nisu mogle jasno definirati niti jednim od analiziranih klimatskih čimbenika.
Najveći utjecaj na vrijednosti obilježja imao je klimatski deficit vlage po Hargreavesu, a zatim najniže
vrijednosti srednje godišnje količine oborina. Evolucijska ekološka istraživanja morfologije iglica jele
vrijedan su doprinos razumijevanju sadašnje genetske varijabilnosti kao preduvjeta za prilagodbu na
brze klimatske promjene i očuvanje vrste na području Balkanskog poluotoka.
PB  - Zagreb: Croatian Forestry Society
T2  - Šumski list
T1  - Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors
T1  - Prostorna varijabilnost morfoloških obilježja iglica populacija jele (Abies alba Mill.) na Balkanskom poluotoku u odnosu na klimatske čimbenike
VL  - 7-8
DO  - 10.31298/SL.146.7-8.3
SP  - 309
EP  - 318
ER  - 

@article{
author = "Popović, Vladan and Šešlija Jovanović, Darka and Lučić, Aleksandar and Rakonjac, Ljubinko and Jovanović, Sanja and Vasiljević, Aleksandar and Miljković, Danijela",
year = "2022",
abstract = "Interpopulation and intrapopulation variability of three morphological needle traits (length, width and thickness) was investigated in 16 natural silver fir populations in the Balkan Peninsula. The populations represent refugial areas of silver fir (Abies alba Mill.). This paper aims to provide a comprehensive analysis of the influence of climatic factors (mean annual temperature, number of days with temperatures < 0, > 5, < 18, > 18oC, Hargreaves climatic moisture deficit and De Martonne aridity index, on the pattern of morphological needle traits within each population. Populations showed variation in the analyzed morphological needle traits, which could not be clearly defined by any of the analyzed climatic factors. The De Martonne aridity index and Hargreaves climatic moisture deficit had the greatest impact on the trait values, whereas the mean annual precipitation had the lowest. Evolutionary ecology research of the silver fir needle morphology is a valuable contribution to the comprehention of the present genetic variability as a prerequisite for adaptation to the rapid climate change and conservation of the species area in the Balkan Peninsula region., Interpopulacijska i intrapopulacijska varijabilnost triju morfoloških osobina iglica (dužina, širina i
debljina) istraživana je u 16 prirodnih populacija jele na Balkanskom poluotoku. Populacije predstav-
ljaju refugijalna područja jele (Abies alba Mill.). Ovaj rad ima za cilj pružiti opsežnu analizu utjecaja
klimatskih čimbenika (srednja godišnja temperatura, broj dana s temperaturama < 0, > 5, < 18, > 18°C,
Hargreavesov klimatski deficit vlage i De Martonneov indeks aridnosti) na obrazac morfoloških
obilježja iglica unutar svake populacije. Populacije su pokazale varijacije u analiziranim morfološkim
obilježjima iglica koje se nisu mogle jasno definirati niti jednim od analiziranih klimatskih čimbenika.
Najveći utjecaj na vrijednosti obilježja imao je klimatski deficit vlage po Hargreavesu, a zatim najniže
vrijednosti srednje godišnje količine oborina. Evolucijska ekološka istraživanja morfologije iglica jele
vrijedan su doprinos razumijevanju sadašnje genetske varijabilnosti kao preduvjeta za prilagodbu na
brze klimatske promjene i očuvanje vrste na području Balkanskog poluotoka.",
publisher = "Zagreb: Croatian Forestry Society",
journal = "Šumski list",
title = "Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors, Prostorna varijabilnost morfoloških obilježja iglica populacija jele (Abies alba Mill.) na Balkanskom poluotoku u odnosu na klimatske čimbenike",
volume = "7-8",
doi = "10.31298/SL.146.7-8.3",
pages = "309-318"
}

Popović, V., Šešlija Jovanović, D., Lučić, A., Rakonjac, L., Jovanović, S., Vasiljević, A.,& Miljković, D.. (2022). Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors. in Šumski list
Zagreb: Croatian Forestry Society., 7-8, 309-318.
https://doi.org/10.31298/SL.146.7-8.3

Popović V, Šešlija Jovanović D, Lučić A, Rakonjac L, Jovanović S, Vasiljević A, Miljković D. Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors. in Šumski list. 2022;7-8:309-318.
doi:10.31298/SL.146.7-8.3 .

Popović, Vladan, Šešlija Jovanović, Darka, Lučić, Aleksandar, Rakonjac, Ljubinko, Jovanović, Sanja, Vasiljević, Aleksandar, Miljković, Danijela, "Spatial variation of morphological needle traits of silver fir (Abies alba Mill.) populations in the Balkan peninsula in relation to climatic factors" in Šumski list, 7-8 (2022):309-318,
https://doi.org/10.31298/SL.146.7-8.3 . .

TY  - JOUR
AU  - Milanović, Slobodan
AU  - Miletić, Zoran
AU  - Marković, Čedomir
AU  - Šešlija Jovanović, Darka
AU  - Trailović, Zoran
AU  - Jankovský, Libor
AU  - Lazarević, Jelica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5014
AB  - Pest resistance of trees should be taken into account in future forest strategy planning and
predicting risks of defoliation. The gypsy moth (GM) (Lymantria dispar L.) is a serious forest pest
with outbreaking population dynamics. To estimate defoliation risk of the most widely distributed
tree species in Balkan Peninsula and Europe (Turkey oak Quercus cerris L., European beech Fagus
sylvatica L. and hornbeam Carpinus betulus L.), we carried out laboratory feeding trials and investigated
their acceptability and suitability for GM development. We determined morphological and
chemical attributes of these hosts as well as larval host preference, growth and nutritional indices.
Preference, growth, and efficiency of food conversion into biomass were ranked in the order: Turkey
oak > European beech > hornbeam. Hornbeam was the most avoided and showed the lowest conversion
efficiency although, comparing to optimal oak host, its leaves were less tough, contained more
water and exhibited similar values of nitrogen (index of protein content) and C/N ratio (index of
investment into carbon based plant defense). We suggest that hornbeam and beech leaf chemical
profiles should be further studied to reveal specific compounds that impose high metabolic cost
to GM larvae. Moreover, additional research are needed to understand how intermediate hosts in
natural populations affect GM outbreaks.
PB  - Basel: MDPI
T2  - Forests
T1  - Suitability of Turkey Oak, European Beech, and Hornbeam to Gypsy Moth Feeding
IS  - 7
VL  - 13
DO  - 10.3390/f13071006
SP  - 1006
ER  - 

@article{
author = "Milanović, Slobodan and Miletić, Zoran and Marković, Čedomir and Šešlija Jovanović, Darka and Trailović, Zoran and Jankovský, Libor and Lazarević, Jelica",
year = "2022",
abstract = "Pest resistance of trees should be taken into account in future forest strategy planning and
predicting risks of defoliation. The gypsy moth (GM) (Lymantria dispar L.) is a serious forest pest
with outbreaking population dynamics. To estimate defoliation risk of the most widely distributed
tree species in Balkan Peninsula and Europe (Turkey oak Quercus cerris L., European beech Fagus
sylvatica L. and hornbeam Carpinus betulus L.), we carried out laboratory feeding trials and investigated
their acceptability and suitability for GM development. We determined morphological and
chemical attributes of these hosts as well as larval host preference, growth and nutritional indices.
Preference, growth, and efficiency of food conversion into biomass were ranked in the order: Turkey
oak > European beech > hornbeam. Hornbeam was the most avoided and showed the lowest conversion
efficiency although, comparing to optimal oak host, its leaves were less tough, contained more
water and exhibited similar values of nitrogen (index of protein content) and C/N ratio (index of
investment into carbon based plant defense). We suggest that hornbeam and beech leaf chemical
profiles should be further studied to reveal specific compounds that impose high metabolic cost
to GM larvae. Moreover, additional research are needed to understand how intermediate hosts in
natural populations affect GM outbreaks.",
publisher = "Basel: MDPI",
journal = "Forests",
title = "Suitability of Turkey Oak, European Beech, and Hornbeam to Gypsy Moth Feeding",
number = "7",
volume = "13",
doi = "10.3390/f13071006",
pages = "1006"
}

Milanović, S., Miletić, Z., Marković, Č., Šešlija Jovanović, D., Trailović, Z., Jankovský, L.,& Lazarević, J.. (2022). Suitability of Turkey Oak, European Beech, and Hornbeam to Gypsy Moth Feeding. in Forests
Basel: MDPI., 13(7), 1006.
https://doi.org/10.3390/f13071006

Milanović S, Miletić Z, Marković Č, Šešlija Jovanović D, Trailović Z, Jankovský L, Lazarević J. Suitability of Turkey Oak, European Beech, and Hornbeam to Gypsy Moth Feeding. in Forests. 2022;13(7):1006.
doi:10.3390/f13071006 .

Milanović, Slobodan, Miletić, Zoran, Marković, Čedomir, Šešlija Jovanović, Darka, Trailović, Zoran, Jankovský, Libor, Lazarević, Jelica, "Suitability of Turkey Oak, European Beech, and Hornbeam to Gypsy Moth Feeding" in Forests, 13, no. 7 (2022):1006,
https://doi.org/10.3390/f13071006 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Kostić, Miroslav
AU  - Šešlija Jovanović, Darka
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4799
AB  - Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.
PB  - Lausanne: Frontiers Media SA
T2  - Frontiers in Physiology
T1  - Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say
VL  - 13
DO  - 10.3389/fphys.2022.842314
SP  - 842314
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Vuleta, Ana and Manitašević Jovanović, Sanja and Kostić, Miroslav and Šešlija Jovanović, Darka",
year = "2022",
abstract = "Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), is one of the most important pests of the common bean Phaseolus vulgaris L. Without appropriate management it may cause significant seed loss in storages. In search for means of environmentally safe and effective protection of beans we assessed biological activity of thymol, an oxygenated monoterpene present in essential oils of many aromatic plants. We studied contact toxicity of thymol on bean seeds and its effects on adult longevity and emergence in F1 generation. Furthermore, we determined acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT), mixed-function oxidase (MFO), carboxylesterases (CarE) and glutathione S-transferase (GST) activities in response to 24 h exposure of beetles to sublethal and lethal thymol concentrations. Our results showed that thymol decreased adult survival, longevity and percentage of adult emergence. Higher median lethal concentration (LC50) was recorded in females indicating their higher tolerance comparing to males. Overall, activities of SOD, CAT and CarE increased at sublethal and MFO increased at both sublethal and lethal thymol concentrations. On the other hand, GST and AChE activities decreased along with the increase in thymol concentrations from sublethal (1/5 of LC50, 1/2 of LC50) to lethal (LC50). Enzyme responses to the presence of thymol on bean seed were sex-specific. In the control group females had lower CarE and higher SOD, CAT and GST activity than males. In treatment groups, females had much higher CAT activity and much lower CarE activity than males. Our results contribute to deeper understanding of physiological mechanisms underlying thymol toxicity and tolerance which should be taken into account in future formulation of a thymol-based insecticide.",
publisher = "Lausanne: Frontiers Media SA",
journal = "Frontiers in Physiology",
title = "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say",
volume = "13",
doi = "10.3389/fphys.2022.842314",
pages = "842314"
}

Lazarević, J., Jevremović, S., Kostić, I., Vuleta, A., Manitašević Jovanović, S., Kostić, M.,& Šešlija Jovanović, D.. (2022). Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology
Lausanne: Frontiers Media SA., 13, 842314.
https://doi.org/10.3389/fphys.2022.842314

Lazarević J, Jevremović S, Kostić I, Vuleta A, Manitašević Jovanović S, Kostić M, Šešlija Jovanović D. Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say. in Frontiers in Physiology. 2022;13:842314.
doi:10.3389/fphys.2022.842314 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Vuleta, Ana, Manitašević Jovanović, Sanja, Kostić, Miroslav, Šešlija Jovanović, Darka, "Assessment of Sex-Specific Toxicity and Physiological Responses to Thymol in a Common Bean Pest Acanthoscelides obtectus Say" in Frontiers in Physiology, 13 (2022):842314,
https://doi.org/10.3389/fphys.2022.842314 . .

TY  - JOUR
AU  - Popović, Vladan
AU  - Nikolić, Biljana
AU  - Lučić, Aleksandar
AU  - Rakonjac, Ljubinko
AU  - Šešlija Jovanović, Darka
AU  - Miljković, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4792
AB  - Geographical and climatic characteristics (elevation, mean annual temperature, summer heat: moisture index, evaporation,
climatic moisture deficit, growing degree-days, heating degree-days, de Martonne’s aridity index) drive variations in
the morphological and anatomical traits of the Norway spruce (Picea abies L. Karst) needle (needle length, needle thickness,
needle width, epidermis thickness with cuticle, hypodermis height, central bundle diameter, resin duct diameter, and the
number of resin ducts). We studied the pattern of variation in these needle characteristics in 15 Balkan Mountains (BM) and
Dinaric Alps (DA) natural populations (altitudes ranging from 1100 to 1860 and from 980 to 1530 m a.s.l., respectively).
The needle traits showed significant differentiation between mountain regions, between populations and within populations.
The largest contribution of genetic component in phenotypic variation had the needle length, both components had the same
share in the needle width, whereas the environmental component of variability had the largest share in the other needle traits.
The principal component analysis revealed that anatomical needle traits and the climatic conditions contributed to differences
between populations from different mountain regions. An agglomerative hierarchical clustering analysis revealed three
dendogram clusters: three BM populations made one cluster, DA populations made the second, whereas seven populations
close to the BM populations and five close to the DA populations made the third cluster. Patterns of morpho-anatomical
phenotypic variability point to selective mechanisms and adjustments, enable to define the boundaries of climate niches,
and may provide a starting point for conservation program according to climate change projections in the Balkan region.
PB  - Berlin : Springer International
T2  - Trees
T1  - Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient
DO  - 10.1007/s00468-022-02277-1
ER  - 

@article{
author = "Popović, Vladan and Nikolić, Biljana and Lučić, Aleksandar and Rakonjac, Ljubinko and Šešlija Jovanović, Darka and Miljković, Danijela",
year = "2022",
abstract = "Geographical and climatic characteristics (elevation, mean annual temperature, summer heat: moisture index, evaporation,
climatic moisture deficit, growing degree-days, heating degree-days, de Martonne’s aridity index) drive variations in
the morphological and anatomical traits of the Norway spruce (Picea abies L. Karst) needle (needle length, needle thickness,
needle width, epidermis thickness with cuticle, hypodermis height, central bundle diameter, resin duct diameter, and the
number of resin ducts). We studied the pattern of variation in these needle characteristics in 15 Balkan Mountains (BM) and
Dinaric Alps (DA) natural populations (altitudes ranging from 1100 to 1860 and from 980 to 1530 m a.s.l., respectively).
The needle traits showed significant differentiation between mountain regions, between populations and within populations.
The largest contribution of genetic component in phenotypic variation had the needle length, both components had the same
share in the needle width, whereas the environmental component of variability had the largest share in the other needle traits.
The principal component analysis revealed that anatomical needle traits and the climatic conditions contributed to differences
between populations from different mountain regions. An agglomerative hierarchical clustering analysis revealed three
dendogram clusters: three BM populations made one cluster, DA populations made the second, whereas seven populations
close to the BM populations and five close to the DA populations made the third cluster. Patterns of morpho-anatomical
phenotypic variability point to selective mechanisms and adjustments, enable to define the boundaries of climate niches,
and may provide a starting point for conservation program according to climate change projections in the Balkan region.",
publisher = "Berlin : Springer International",
journal = "Trees",
title = "Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient",
doi = "10.1007/s00468-022-02277-1"
}

Popović, V., Nikolić, B., Lučić, A., Rakonjac, L., Šešlija Jovanović, D.,& Miljković, D.. (2022). Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient. in Trees
Berlin : Springer International..
https://doi.org/10.1007/s00468-022-02277-1

Popović V, Nikolić B, Lučić A, Rakonjac L, Šešlija Jovanović D, Miljković D. Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient. in Trees. 2022;.
doi:10.1007/s00468-022-02277-1 .

Popović, Vladan, Nikolić, Biljana, Lučić, Aleksandar, Rakonjac, Ljubinko, Šešlija Jovanović, Darka, Miljković, Danijela, "Morpho‑anatomical trait variability of the Norway spruce (Picea abies (L.) Karst.) needles in natural populations along elevational diversity gradient" in Trees (2022),
https://doi.org/10.1007/s00468-022-02277-1 . .

TY  - JOUR
AU  - Kostić, Igor
AU  - Lazarević, Jelica
AU  - Šešlija Jovanović, Darka
AU  - Kostić, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4504
AB  - The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood
forests. In the search for an environmentally safe means of its control, we assessed the impact
of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise
Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality,
molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with
commercial insecticide NeemAzal®-T/S (neem). The main compounds in the Eos were trans-anethole
in anise; carvone, limonene, and  -phellandrene in dill; and trans-anethole and fenchone in fennel
seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more
toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%,
anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs
were more effective in reducing growth rate, approximate digestibility and efficiency of conversion
of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and
metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from
both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used
for gypsy moth control.
PB  - Basel: MDPI
T2  - Plants
T1  - Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control
IS  - 10
VL  - 10
DO  - 10.3390/plants10102194
SP  - 2194
ER  - 

@article{
author = "Kostić, Igor and Lazarević, Jelica and Šešlija Jovanović, Darka and Kostić, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "The gypsy moth (Lymantria dispar L. (Lepidoptera: Erebidae)) is a serious pest of hardwood
forests. In the search for an environmentally safe means of its control, we assessed the impact
of different concentrations of essential oils (EOs) from the seeds of three Apiaceae plants (anise
Pimpinella anisum, dill Anethum graveolens, and fennel Foeniculum vulgare) on behavior, mortality,
molting and nutritional physiology of gypsy moth larvae (GML). EOs efficacy was compared with
commercial insecticide NeemAzal®-T/S (neem). The main compounds in the Eos were trans-anethole
in anise; carvone, limonene, and  -phellandrene in dill; and trans-anethole and fenchone in fennel
seed. At 1% EOs concentration, anise and fennel were better antifeedants and all three EOs were more
toxic than neem. Neem was superior in delaying 2nd to 3rd larval molting. In the 4th instar, 0.5%,
anise and fennel EOs decreased relative consumption rate more than neem, whereas all three EOs
were more effective in reducing growth rate, approximate digestibility and efficiency of conversion
of food into body mass leading to higher metabolic costs to GML. Decrease in consumption and
metabolic parameters compared to control GML confirmed that adverse effects of the EOs stem from
both pre- and post-ingestive mechanisms. The results indicate the potential of three EOs to be used
for gypsy moth control.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control",
number = "10",
volume = "10",
doi = "10.3390/plants10102194",
pages = "2194"
}

Kostić, I., Lazarević, J., Šešlija Jovanović, D., Kostić, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants
Basel: MDPI., 10(10), 2194.
https://doi.org/10.3390/plants10102194

Kostić I, Lazarević J, Šešlija Jovanović D, Kostić M, Marković T, Milanović S. Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control. in Plants. 2021;10(10):2194.
doi:10.3390/plants10102194 .

Kostić, Igor, Lazarević, Jelica, Šešlija Jovanović, Darka, Kostić, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils from Anise, Dill and Fennel Seeds for the Gypsy Moth Control" in Plants, 10, no. 10 (2021):2194,
https://doi.org/10.3390/plants10102194 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Kostić, Igor
AU  - Milanović, Slobodan
AU  - Šešlija Jovanović, Darka
AU  - Krnjajić, Slobodan
AU  - Ćalić, Dušica
AU  - Stanković, Slađan
AU  - Kostić, Miroslav
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3838
AB  - The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.
PB  - Cambridge University Press
T2  - Bulletin of Entomological Research
T1  - Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)
IS  - 2
VL  - 11
DO  - 10.1017/S0007485320000504
SP  - 190
EP  - 199
ER  - 

@article{
author = "Lazarević, Jelica and Kostić, Igor and Milanović, Slobodan and Šešlija Jovanović, Darka and Krnjajić, Slobodan and Ćalić, Dušica and Stanković, Slađan and Kostić, Miroslav",
year = "2021",
abstract = "The Colorado potato beetle, Leptinotarsa decemlineata (Say), is one of the most destructive pest species to have developed resistance to most chemical insecticides. We determined the composition and evaluated the potential of Tanacetum parthenium L. and Tanacetum vulgare L. (Asteraceae family) essential oil (EO) application as an alternative eco-friendly control strategy against L. decemlineata. We assessed the antifeedant activity for L. decemlineata larvae and adults by estimating the damage to potato leaves treated with three concentrations of EOs dissolved in ethanol (0.125, 0.25 and 0.5%). Results showed that T. parthenium EO was more effective against larvae, and T. vulgare was more effective against adults. In an olfactometer assay, the time required to choose an untreated leaf disc did not depend on the Tanacetum species, or life stage examined. However, the concentration of EO exhibited a significant effect on the behaviour of both developmental stages. At higher EO concentrations, both third instar larvae and adults require less time to choose an untreated leaf disc. Additionally, T. parthenium EO provoked more rapid movement away from the treated leaf disc than T. vulgare, especially at the highest concentration. Successful modification of L. decemlineata behaviour by the two Tanacetum oils suggests that they possess the potential for use in potato protection.",
publisher = "Cambridge University Press",
journal = "Bulletin of Entomological Research",
title = "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)",
number = "2",
volume = "11",
doi = "10.1017/S0007485320000504",
pages = "190-199"
}

Lazarević, J., Kostić, I., Milanović, S., Šešlija Jovanović, D., Krnjajić, S., Ćalić, D., Stanković, S.,& Kostić, M.. (2021). Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research
Cambridge University Press., 11(2), 190-199.
https://doi.org/10.1017/S0007485320000504

Lazarević J, Kostić I, Milanović S, Šešlija Jovanović D, Krnjajić S, Ćalić D, Stanković S, Kostić M. Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say). in Bulletin of Entomological Research. 2021;11(2):190-199.
doi:10.1017/S0007485320000504 .

Lazarević, Jelica, Kostić, Igor, Milanović, Slobodan, Šešlija Jovanović, Darka, Krnjajić, Slobodan, Ćalić, Dušica, Stanković, Slađan, Kostić, Miroslav, "Repellent activity of Tanacetum parthenium (L.) and Tanacetum vulgare (L.) essential oils against Leptinotarsa decemlineata (Say)" in Bulletin of Entomological Research, 11, no. 2 (2021):190-199,
https://doi.org/10.1017/S0007485320000504 . .

TY  - DATA
AU  - Kostić, igor
AU  - Lazarević, Jelica
AU  - Šešlija Jovanović, Darka
AU  - Kostić, Miroslav
AU  - Marković, Tatjana
AU  - Milanović, Slobodan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4465
AB  - We provide one excel (with four sheets) and 4 csv files. The data in the excel is the same as the one provided in the 4 csv files. 

Data file named 'data plants 1393603.xls' is consisted of 4 sheets containing raw data on all analyzed traits in the manuscript. 

The first sheet named 'antifeeding' contains data on absolute and relative deterrence coefficients of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The second sheet named 'mortality' contains data on the percentage of mortality of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The third sheet named 'molting' contains data on the percentage of 2nd to 3rd instar molting and percentage of molting reduction of gypsy moth larvae exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The forth sheet named 'growth and nutritional indices' contains data on the initial mass, amount of consumed, assimilated and metabolized food, and indices RGR, RCR, RMR, ECI, AD, ECD, and metabolic cost of gypsy moth larvae (4th instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard.
PB  - Basel: MDPI
T2  - Plants
T1  - Potential of Essential Oils for the Gypsy Moth Control
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4465
ER  - 

@misc{
author = "Kostić, igor and Lazarević, Jelica and Šešlija Jovanović, Darka and Kostić, Miroslav and Marković, Tatjana and Milanović, Slobodan",
year = "2021",
abstract = "We provide one excel (with four sheets) and 4 csv files. The data in the excel is the same as the one provided in the 4 csv files. 

Data file named 'data plants 1393603.xls' is consisted of 4 sheets containing raw data on all analyzed traits in the manuscript. 

The first sheet named 'antifeeding' contains data on absolute and relative deterrence coefficients of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The second sheet named 'mortality' contains data on the percentage of mortality of gypsy moth larvae (2nd instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The third sheet named 'molting' contains data on the percentage of 2nd to 3rd instar molting and percentage of molting reduction of gypsy moth larvae exposed to different concentrations of anise, dill and fennel essential oils and neem standard. 

The forth sheet named 'growth and nutritional indices' contains data on the initial mass, amount of consumed, assimilated and metabolized food, and indices RGR, RCR, RMR, ECI, AD, ECD, and metabolic cost of gypsy moth larvae (4th instar) exposed to different concentrations of anise, dill and fennel essential oils and neem standard.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "Potential of Essential Oils for the Gypsy Moth Control",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4465"
}

Kostić, i., Lazarević, J., Šešlija Jovanović, D., Kostić, M., Marković, T.,& Milanović, S.. (2021). Potential of Essential Oils for the Gypsy Moth Control. in Plants
Basel: MDPI..
https://hdl.handle.net/21.15107/rcub_ibiss_4465

Kostić I, Lazarević J, Šešlija Jovanović D, Kostić M, Marković T, Milanović S. Potential of Essential Oils for the Gypsy Moth Control. in Plants. 2021;.
https://hdl.handle.net/21.15107/rcub_ibiss_4465 .

Kostić, igor, Lazarević, Jelica, Šešlija Jovanović, Darka, Kostić, Miroslav, Marković, Tatjana, Milanović, Slobodan, "Potential of Essential Oils for the Gypsy Moth Control" in Plants (2021),
https://hdl.handle.net/21.15107/rcub_ibiss_4465 .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Jevremović, Stojan
AU  - Kostić, Igor
AU  - Kostić, Miroslav
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Šešlija Jovanović, Darka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3837
AB  - The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Insects
T1  - Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus
IS  - 9
VL  - 11
DO  - 10.3390/insects11090563
SP  - 563
ER  - 

@article{
author = "Lazarević, Jelica and Jevremović, Stojan and Kostić, Igor and Kostić, Miroslav and Vuleta, Ana and Manitašević Jovanović, Sanja and Šešlija Jovanović, Darka",
year = "2020",
abstract = "The bean weevil Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae) can cause significant losses in production of its primary host common bean Phaseolus vulgaris L. To avoid bean protection with environmentally risky chemical insecticides and provide sustainable and safe production of food, new pest management methods based on natural compounds are investigated. In the present study, we evaluated protective potential of the essential oil (EO) from the common thyme Thymus vulgaris L. applied on bean seeds. We assessed residual contact toxicity of thyme EO and its effects on A. obtectus longevity, oviposition and adult emergence. Furthermore, to elucidate the role of oxidative stress in thyme EO toxicity, we estimated the levels of oxidatively damaged proteins and lipids, as well as the level of thiols which have important role for antioxidant capacity. We found that thyme oil significantly reduced adult survival and longevity, induced oxidative damage to lipids and proteins and depleted protein and non-protein thiols in a concentration-dependent manner. Females appeared to be more tolerant to thyme oil treatment than males. Sublethal EO concentrations affected oxidative stress indices, deterred oviposition and strongly inhibited adult emergence. The results suggest that thyme oil has the potential to be used as an ecofriendly insecticide for A. obtectus control.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Insects",
title = "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus",
number = "9",
volume = "11",
doi = "10.3390/insects11090563",
pages = "563"
}

Lazarević, J., Jevremović, S., Kostić, I., Kostić, M., Vuleta, A., Manitašević Jovanović, S.,& Šešlija Jovanović, D.. (2020). Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects
Multidisciplinary Digital Publishing Institute., 11(9), 563.
https://doi.org/10.3390/insects11090563

Lazarević J, Jevremović S, Kostić I, Kostić M, Vuleta A, Manitašević Jovanović S, Šešlija Jovanović D. Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus. in Insects. 2020;11(9):563.
doi:10.3390/insects11090563 .

Lazarević, Jelica, Jevremović, Stojan, Kostić, Igor, Kostić, Miroslav, Vuleta, Ana, Manitašević Jovanović, Sanja, Šešlija Jovanović, Darka, "Toxic, Oviposition Deterrent and Oxidative Stress Effects of Thymus vulgaris Essential Oil against Acanthoscelides obtectus" in Insects, 11, no. 9 (2020):563,
https://doi.org/10.3390/insects11090563 . .

TY  - JOUR
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Šešlija Jovanović, Darka
AU  - Lazarević, Jelica
AU  - Tucić, Nikola
AU  - Stojković, Biljana
PY  - 2016
UR  - http://onlinelibrary.wiley.com/doi/10.1111/jeb.12831/abstract
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26790127
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2925
AB  - Expansion of the host range in phytophagous insects depends on their ability to form an association with a novel plant through changes in host-related traits. Phenotypic plasticity has important effects on initial survival of individuals faced with a new plant, as well as on the courses of evolutionary change during long-term adaptation to novel conditions. Using experimental populations of the seed beetle that evolved on ancestral (common bean) or novel (chickpea) host and applying reciprocal transplant at both larval and adult stage on the alternative host plant, we studied the relationship between the initial (plastic) phases of host-shift and the subsequent stages of evolutionary divergence in life-history strategies between populations exposed to the host-shift process. After 48 generations, populations became well adapted to chickpea by evolving the life-history strategy with prolonged larval development, increased body mass, earlier reproduction, shorter lifespan and decreased plasticity of all traits compared with ancestral conditions. In chickpea-adapted beetles, negative fitness consequences of low plasticity of pre-adult development (revealed as severe decrease in egg-to-adult viability on beans) exhibited mismatch with positive effects of low plasticity (i.e. low host sensitivity) in oviposition and fecundity. In contrast, beetles adapted to the ancestral host showed high plasticity of developmental process, which enabled high larval survival on chickpea, whereas elevated plasticity in adult behaviour (i.e. high host sensitivity) resulted in delayed reproduction and decreased fecundity on chickpea. The analysis of population growth parameters revealed significant fluctuation during successive phases of the host-shift process in A. obtectus.
T2  - Journal of Evolutionary Biology
T1  - Experimentally induced host-shift changes life-history strategy in a seed beetle.
IS  - 4
VL  - 29
DO  - 10.1111/jeb.12831
SP  - 837
EP  - 47
ER  - 

@article{
author = "Savković, Uroš and Đorđević, Mirko and Šešlija Jovanović, Darka and Lazarević, Jelica and Tucić, Nikola and Stojković, Biljana",
year = "2016",
abstract = "Expansion of the host range in phytophagous insects depends on their ability to form an association with a novel plant through changes in host-related traits. Phenotypic plasticity has important effects on initial survival of individuals faced with a new plant, as well as on the courses of evolutionary change during long-term adaptation to novel conditions. Using experimental populations of the seed beetle that evolved on ancestral (common bean) or novel (chickpea) host and applying reciprocal transplant at both larval and adult stage on the alternative host plant, we studied the relationship between the initial (plastic) phases of host-shift and the subsequent stages of evolutionary divergence in life-history strategies between populations exposed to the host-shift process. After 48 generations, populations became well adapted to chickpea by evolving the life-history strategy with prolonged larval development, increased body mass, earlier reproduction, shorter lifespan and decreased plasticity of all traits compared with ancestral conditions. In chickpea-adapted beetles, negative fitness consequences of low plasticity of pre-adult development (revealed as severe decrease in egg-to-adult viability on beans) exhibited mismatch with positive effects of low plasticity (i.e. low host sensitivity) in oviposition and fecundity. In contrast, beetles adapted to the ancestral host showed high plasticity of developmental process, which enabled high larval survival on chickpea, whereas elevated plasticity in adult behaviour (i.e. high host sensitivity) resulted in delayed reproduction and decreased fecundity on chickpea. The analysis of population growth parameters revealed significant fluctuation during successive phases of the host-shift process in A. obtectus.",
journal = "Journal of Evolutionary Biology",
title = "Experimentally induced host-shift changes life-history strategy in a seed beetle.",
number = "4",
volume = "29",
doi = "10.1111/jeb.12831",
pages = "837-47"
}

Savković, U., Đorđević, M., Šešlija Jovanović, D., Lazarević, J., Tucić, N.,& Stojković, B.. (2016). Experimentally induced host-shift changes life-history strategy in a seed beetle.. in Journal of Evolutionary Biology, 29(4), 837-47.
https://doi.org/10.1111/jeb.12831

Savković U, Đorđević M, Šešlija Jovanović D, Lazarević J, Tucić N, Stojković B. Experimentally induced host-shift changes life-history strategy in a seed beetle.. in Journal of Evolutionary Biology. 2016;29(4):837-47.
doi:10.1111/jeb.12831 .

Savković, Uroš, Đorđević, Mirko, Šešlija Jovanović, Darka, Lazarević, Jelica, Tucić, Nikola, Stojković, Biljana, "Experimentally induced host-shift changes life-history strategy in a seed beetle." in Journal of Evolutionary Biology, 29, no. 4 (2016):837-47,
https://doi.org/10.1111/jeb.12831 . .

TY  - JOUR
AU  - Janković Tomanić, Milena
AU  - Šešlija Jovanović, Darka
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Lazarević, Jelica
PY  - 2015
UR  - http://www.sciencedirect.com/science/article/pii/S0022474X15000272?via%3Dihub#ack0010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2922
AB  - Bean weevil, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae, Bruchinae) is a cosmopolitan pest of legume stored products. Storages with various legume seeds can facilitate shifts of the weevil from its primary host, common bean (Phaseolus vulgaris L.), to other legume species and enable host expansion, i.e. broadening of the range of acceptable plant-hosts. In the first generation of host shift, survival of an insect depends on ability to adjust its physiology to altered content of nutrients and secondary metabolites in novel host. On a long-term scale, physiological adaptations to a new host can comprise modifications both in level and plasticity of physiological traits. Changes in activity of phosphatases play an essential role in this process due to their involvement in diverse functions. This study investigated alterations in activity of total acid, lysosomal acid and alkaline phosphatases using laboratory populations of A.obtectus which were maintained either on the optimal host (common bean) or on the suboptimal host (chickpea, Cicer arietinum L.) for 28 years. To determine short-term (plastic) effects, subsets of individuals from each population were exposed to the alternative host for one generation. Our results revealed that one-generation shift to chickpea significantly increased phosphatases' activity reflecting immediate plastic response to nutritional/allelochemical stress where these enzymes might be involved in defense mechanisms. On the other hand, both level and plasticity of phosphatases' activities significantly declined as a long-term response to Cicer-based diet suggesting that selection on chickpea favored resistance mechanisms that were less costly than phosphatases. Considering diverse roles of phosphatases we suggest that such modifications could be crucial for expanding host range and might have implications on efficiency of chemical and botanical insecticides.
T2  - Journal of Stored Products Research
T1  - Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say)
VL  - 62
DO  - 10.1016/j.jspr.2015.03.008
SP  - 32
EP  - 35
ER  - 

@article{
author = "Janković Tomanić, Milena and Šešlija Jovanović, Darka and Savković, Uroš and Đorđević, Mirko and Stojković, Biljana and Lazarević, Jelica",
year = "2015",
abstract = "Bean weevil, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae, Bruchinae) is a cosmopolitan pest of legume stored products. Storages with various legume seeds can facilitate shifts of the weevil from its primary host, common bean (Phaseolus vulgaris L.), to other legume species and enable host expansion, i.e. broadening of the range of acceptable plant-hosts. In the first generation of host shift, survival of an insect depends on ability to adjust its physiology to altered content of nutrients and secondary metabolites in novel host. On a long-term scale, physiological adaptations to a new host can comprise modifications both in level and plasticity of physiological traits. Changes in activity of phosphatases play an essential role in this process due to their involvement in diverse functions. This study investigated alterations in activity of total acid, lysosomal acid and alkaline phosphatases using laboratory populations of A.obtectus which were maintained either on the optimal host (common bean) or on the suboptimal host (chickpea, Cicer arietinum L.) for 28 years. To determine short-term (plastic) effects, subsets of individuals from each population were exposed to the alternative host for one generation. Our results revealed that one-generation shift to chickpea significantly increased phosphatases' activity reflecting immediate plastic response to nutritional/allelochemical stress where these enzymes might be involved in defense mechanisms. On the other hand, both level and plasticity of phosphatases' activities significantly declined as a long-term response to Cicer-based diet suggesting that selection on chickpea favored resistance mechanisms that were less costly than phosphatases. Considering diverse roles of phosphatases we suggest that such modifications could be crucial for expanding host range and might have implications on efficiency of chemical and botanical insecticides.",
journal = "Journal of Stored Products Research",
title = "Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say)",
volume = "62",
doi = "10.1016/j.jspr.2015.03.008",
pages = "32-35"
}

Janković Tomanić, M., Šešlija Jovanović, D., Savković, U., Đorđević, M., Stojković, B.,& Lazarević, J.. (2015). Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say). in Journal of Stored Products Research, 62, 32-35.
https://doi.org/10.1016/j.jspr.2015.03.008

Janković Tomanić M, Šešlija Jovanović D, Savković U, Đorđević M, Stojković B, Lazarević J. Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say). in Journal of Stored Products Research. 2015;62:32-35.
doi:10.1016/j.jspr.2015.03.008 .

Janković Tomanić, Milena, Šešlija Jovanović, Darka, Savković, Uroš, Đorđević, Mirko, Stojković, Biljana, Lazarević, Jelica, "Host expansion modifies activity of phosphatases in a legume store pest Acanthoscelides obtectus (Say)" in Journal of Stored Products Research, 62 (2015):32-35,
https://doi.org/10.1016/j.jspr.2015.03.008 . .

TY  - JOUR
AU  - Cingel, Aleksandar
AU  - Savic, Jelena
AU  - Vinterhalter, Branka
AU  - Vinterhalter, Dragan
AU  - Kostic, Miroslav
AU  - Šešlija Jovanović, Darka
AU  - Smigocki, Ann
AU  - Ninković, Slavica
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1923
AB  - Plant proteinase inhibitors (PIs) are attractive tools for crop
   improvement and their heterologous expression can enhance insect
   resistance in transgenic plants. PI oryzacystatin II (OCII), isolated
   from rice, showed potential in controlling pests that utilize cysteine
   proteinases for protein digestion. To evaluate the applicability of the
   OCII gene in enhancing plant defence, OCII-transformed potatoes were
   bioassayed for resistance to Colorado potato beetle (Leptinotarsa
   decemlineata Say). Feeding on transformed leaves of potato cultivars
   Desiree and Jelica significantly affected larval growth and development,
   but did not change mortality rates. During the L2 and L3 developmental
   stages larvae consumed the OCII-transformed foliage faster as compared
   to the nontransformed control. Also these larvae reached the prepupal
   stage (end of L4 stage) 2 days earlier than those fed on control leaves.
   However, the total amounts of consumed OCII-transformed leaves were up
   to 23 \% lower than of control, and the maximal weights of prepupal
   larvae were reduced by up to 18 \% as compared to larvae fed on
   nontransformed leaves. The reduction in insect fitness reported in this
   study in combination with other control measures, could lead to improved
   CPB resistance management in potato.
T2  - Transgenic Research
T1  - Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor
IS  - 4
VL  - 24
DO  - 10.1007/s11248-015-9873-9
SP  - 729
EP  - 740
ER  - 

@article{
author = "Cingel, Aleksandar and Savic, Jelena and Vinterhalter, Branka and Vinterhalter, Dragan and Kostic, Miroslav and Šešlija Jovanović, Darka and Smigocki, Ann and Ninković, Slavica",
year = "2015",
abstract = "Plant proteinase inhibitors (PIs) are attractive tools for crop
   improvement and their heterologous expression can enhance insect
   resistance in transgenic plants. PI oryzacystatin II (OCII), isolated
   from rice, showed potential in controlling pests that utilize cysteine
   proteinases for protein digestion. To evaluate the applicability of the
   OCII gene in enhancing plant defence, OCII-transformed potatoes were
   bioassayed for resistance to Colorado potato beetle (Leptinotarsa
   decemlineata Say). Feeding on transformed leaves of potato cultivars
   Desiree and Jelica significantly affected larval growth and development,
   but did not change mortality rates. During the L2 and L3 developmental
   stages larvae consumed the OCII-transformed foliage faster as compared
   to the nontransformed control. Also these larvae reached the prepupal
   stage (end of L4 stage) 2 days earlier than those fed on control leaves.
   However, the total amounts of consumed OCII-transformed leaves were up
   to 23 \% lower than of control, and the maximal weights of prepupal
   larvae were reduced by up to 18 \% as compared to larvae fed on
   nontransformed leaves. The reduction in insect fitness reported in this
   study in combination with other control measures, could lead to improved
   CPB resistance management in potato.",
journal = "Transgenic Research",
title = "Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor",
number = "4",
volume = "24",
doi = "10.1007/s11248-015-9873-9",
pages = "729-740"
}

Cingel, A., Savic, J., Vinterhalter, B., Vinterhalter, D., Kostic, M., Šešlija Jovanović, D., Smigocki, A.,& Ninković, S.. (2015). Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor. in Transgenic Research, 24(4), 729-740.
https://doi.org/10.1007/s11248-015-9873-9

Cingel A, Savic J, Vinterhalter B, Vinterhalter D, Kostic M, Šešlija Jovanović D, Smigocki A, Ninković S. Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor. in Transgenic Research. 2015;24(4):729-740.
doi:10.1007/s11248-015-9873-9 .

Cingel, Aleksandar, Savic, Jelena, Vinterhalter, Branka, Vinterhalter, Dragan, Kostic, Miroslav, Šešlija Jovanović, Darka, Smigocki, Ann, Ninković, Slavica, "Growth and development of Colorado potato beetle larvae, Leptinotarsa
 decemlineata, on potato plants expressing the oryzacystatin II
 proteinase inhibitor" in Transgenic Research, 24, no. 4 (2015):729-740,
https://doi.org/10.1007/s11248-015-9873-9 . .

TY  - JOUR
AU  - Šešlija Jovanović, Darka
AU  - Đorđević, Mirko
AU  - Savković, Uroš
AU  - Lazarević, Jelica
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2152
AB  - Mitochondria are suggested to play a central role in ageing and
   evolution of longevity. Gradual decline in mitochondrial function during
   ageing and concomitant increase in production of reactive oxygen species
   (ROS) leads to oxidative damage of macromolecules and impairment of ATP
   synthesis. To assess relationship between ageing and oxidative stress
   resistance we exposed different longevity lines of the seed beetle
   (Acanthoscelides obtectus) to four concentrations of tebufenpyrad,
   mitochondrial complex I inhibitor. Complex I is one of main sites of ROS
   production during normal respiration and its inhibition elevates
   oxidative stress. Our results showed that 24 h of exposure to
   tebufenpyrad decreased survival and post-stress longevity due to
   increased baseline mortality. Higher resistance was recorded in beetles
   from lines selected for late reproduction and extended longevity (L)
   than in early reproducing beetles (E). Also, females were more resistant
   than males. Since complex I is under dual genetic control, our second
   aim was to disentangle relative contribution of nuclear and
   mitochondrial genes to the variation in longevity. We used crossed
   combinations of distinct mitochondrial and nuclear genotypes (E x L, L x
   E) and compared them to control hybrids where mitochondrial genome was
   ``transplanted{''} onto the original background (E x E, L x L). Our
   study revealed significant effect of nucleus, i.e. higher survival and
   post-stress longevity in beetles harbouring L nucleus. Mitochondrion
   effect was significant only within L nuclear background where E
   mitochondrion gave advantage.
T2  - Biogerontology
T1  - The effect of mitochondrial complex I inhibitor on longevity of
 short-lived and long-lived seed beetles and its mitonuclear hybrids
IS  - 5
VL  - 15
DO  - 10.1007/s10522-014-9520-5
SP  - 487
EP  - 501
ER  - 

@article{
author = "Šešlija Jovanović, Darka and Đorđević, Mirko and Savković, Uroš and Lazarević, Jelica",
year = "2014",
abstract = "Mitochondria are suggested to play a central role in ageing and
   evolution of longevity. Gradual decline in mitochondrial function during
   ageing and concomitant increase in production of reactive oxygen species
   (ROS) leads to oxidative damage of macromolecules and impairment of ATP
   synthesis. To assess relationship between ageing and oxidative stress
   resistance we exposed different longevity lines of the seed beetle
   (Acanthoscelides obtectus) to four concentrations of tebufenpyrad,
   mitochondrial complex I inhibitor. Complex I is one of main sites of ROS
   production during normal respiration and its inhibition elevates
   oxidative stress. Our results showed that 24 h of exposure to
   tebufenpyrad decreased survival and post-stress longevity due to
   increased baseline mortality. Higher resistance was recorded in beetles
   from lines selected for late reproduction and extended longevity (L)
   than in early reproducing beetles (E). Also, females were more resistant
   than males. Since complex I is under dual genetic control, our second
   aim was to disentangle relative contribution of nuclear and
   mitochondrial genes to the variation in longevity. We used crossed
   combinations of distinct mitochondrial and nuclear genotypes (E x L, L x
   E) and compared them to control hybrids where mitochondrial genome was
   ``transplanted{''} onto the original background (E x E, L x L). Our
   study revealed significant effect of nucleus, i.e. higher survival and
   post-stress longevity in beetles harbouring L nucleus. Mitochondrion
   effect was significant only within L nuclear background where E
   mitochondrion gave advantage.",
journal = "Biogerontology",
title = "The effect of mitochondrial complex I inhibitor on longevity of
 short-lived and long-lived seed beetles and its mitonuclear hybrids",
number = "5",
volume = "15",
doi = "10.1007/s10522-014-9520-5",
pages = "487-501"
}

Šešlija Jovanović, D., Đorđević, M., Savković, U.,& Lazarević, J.. (2014). The effect of mitochondrial complex I inhibitor on longevity of
 short-lived and long-lived seed beetles and its mitonuclear hybrids. in Biogerontology, 15(5), 487-501.
https://doi.org/10.1007/s10522-014-9520-5

Šešlija Jovanović D, Đorđević M, Savković U, Lazarević J. The effect of mitochondrial complex I inhibitor on longevity of
 short-lived and long-lived seed beetles and its mitonuclear hybrids. in Biogerontology. 2014;15(5):487-501.
doi:10.1007/s10522-014-9520-5 .

Šešlija Jovanović, Darka, Đorđević, Mirko, Savković, Uroš, Lazarević, Jelica, "The effect of mitochondrial complex I inhibitor on longevity of
 short-lived and long-lived seed beetles and its mitonuclear hybrids" in Biogerontology, 15, no. 5 (2014):487-501,
https://doi.org/10.1007/s10522-014-9520-5 . .

TY  - JOUR
AU  - Stojković, Biljana
AU  - Šešlija-Jovanović, Darka M.
AU  - Tucić, Nikola G
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1229
AB  - Oviposition behaviour is recognized as an ecologically significant trait in insects. Since herbivorous insect females have a potential to lay eggs on unsuitable host species, it is hypothesized that this behaviour may be the first step in host-range expansion. The most intriguing phenomenon, found in some insect species, is the ability of females to lay eggs on unsuitable places if hosts are unavailable; this behaviour is termed 'egg-dumping: Egg-dumping can be adaptive plastic response in environments where hosts, either preferred or less suitable, may eventually become available. Transgenerational effects of this behaviour presume changes in larval feeding experience on different host species. Also, diverse larval experience may induce alterations in oviposition behaviour. To test whether egg laying behaviour of adult females can be modified by larval experience, we performed the experiment on two laboratory populations of Acanthoscelides obtectus (Say). First population (P) is housed in the laboratory on the common bean for 200 generations, whereas the second population (C) is derived from the first but has been raised for 197 generations on chickpea, a novel and less suitable host for larval development. In order to analyze differences between short-term and long-term effects of larval experience on egg-dumping, both populations have been exposed to each host seed for one generation. The results of the study show that females from P population lay eggs more frequently in the absence of seeds when developed on chickpeas, compared with P females reared on beans. By contrast to P females, in C population, larval experience has no significant effects either on the number of dumped eggs or on the frequency of dumper females. These results demonstrate a significant inter-population diversification of phenotypic plasticity in egg-dumping behaviour depending on larval host experience. In other words, modification of oviposition behaviour, as a consequence of a long-term larval nutritional stress, can evoke previously unrecognized transgenerational effects.
T2  - Polish Journal of Ecology
T1  - Transgenerational Effects on Overall Fitness: Influence of Larval Feeding Experience on the Oviposition Behaviour of Seed Beetle Acanthoscelides Obtectus (Say)
IS  - 2
VL  - 60
EP  - 393
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1229
ER  - 

@article{
author = "Stojković, Biljana and Šešlija-Jovanović, Darka M. and Tucić, Nikola G",
year = "2012",
abstract = "Oviposition behaviour is recognized as an ecologically significant trait in insects. Since herbivorous insect females have a potential to lay eggs on unsuitable host species, it is hypothesized that this behaviour may be the first step in host-range expansion. The most intriguing phenomenon, found in some insect species, is the ability of females to lay eggs on unsuitable places if hosts are unavailable; this behaviour is termed 'egg-dumping: Egg-dumping can be adaptive plastic response in environments where hosts, either preferred or less suitable, may eventually become available. Transgenerational effects of this behaviour presume changes in larval feeding experience on different host species. Also, diverse larval experience may induce alterations in oviposition behaviour. To test whether egg laying behaviour of adult females can be modified by larval experience, we performed the experiment on two laboratory populations of Acanthoscelides obtectus (Say). First population (P) is housed in the laboratory on the common bean for 200 generations, whereas the second population (C) is derived from the first but has been raised for 197 generations on chickpea, a novel and less suitable host for larval development. In order to analyze differences between short-term and long-term effects of larval experience on egg-dumping, both populations have been exposed to each host seed for one generation. The results of the study show that females from P population lay eggs more frequently in the absence of seeds when developed on chickpeas, compared with P females reared on beans. By contrast to P females, in C population, larval experience has no significant effects either on the number of dumped eggs or on the frequency of dumper females. These results demonstrate a significant inter-population diversification of phenotypic plasticity in egg-dumping behaviour depending on larval host experience. In other words, modification of oviposition behaviour, as a consequence of a long-term larval nutritional stress, can evoke previously unrecognized transgenerational effects.",
journal = "Polish Journal of Ecology",
title = "Transgenerational Effects on Overall Fitness: Influence of Larval Feeding Experience on the Oviposition Behaviour of Seed Beetle Acanthoscelides Obtectus (Say)",
number = "2",
volume = "60",
pages = "393",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1229"
}

Stojković, B., Šešlija-Jovanović, D. M.,& Tucić, N. G.. (2012). Transgenerational Effects on Overall Fitness: Influence of Larval Feeding Experience on the Oviposition Behaviour of Seed Beetle Acanthoscelides Obtectus (Say). in Polish Journal of Ecology, 60(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1229

Stojković B, Šešlija-Jovanović DM, Tucić NG. Transgenerational Effects on Overall Fitness: Influence of Larval Feeding Experience on the Oviposition Behaviour of Seed Beetle Acanthoscelides Obtectus (Say). in Polish Journal of Ecology. 2012;60(2):null-393.
https://hdl.handle.net/21.15107/rcub_ibiss_1229 .

Stojković, Biljana, Šešlija-Jovanović, Darka M., Tucić, Nikola G, "Transgenerational Effects on Overall Fitness: Influence of Larval Feeding Experience on the Oviposition Behaviour of Seed Beetle Acanthoscelides Obtectus (Say)" in Polish Journal of Ecology, 60, no. 2 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1229 .

TY  - JOUR
AU  - Stojković, Biljana
AU  - Šešlija-Jovanović, Darka M.
AU  - Perovanović, Jelena
AU  - Tucić, Nikola G
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1271
AB  - We evaluated the degree of reproductive isolation between laboratory populations of the seed beetle (Acanthoscelides obtectus) selected to reproduce early (E) or late (L) in life, where different levels of sexual activity and sexual discrimination have been detected. We found a significant level of behavioral isolation among populations within the E selection regime in which beetles showed enhanced early-life fitness traits and low sexual activity. In contrast, substantially higher levels of sexual activity and an indiscriminate mating system inhibited rather than promoted pre-zygotic isolation between the L populations. Our results indicate that the study of sexual activity levels may be crucial for understanding the first steps in the pre-zygotic isolation among allopatric populations subjected to uniform selection.
T2  - Ethology
T1  - Sexual Activity and Reproductive Isolation Between Age-specific Selected Populations of Seed Beetle
IS  - 9
VL  - 117
EP  - 821
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1271
ER  - 

@article{
author = "Stojković, Biljana and Šešlija-Jovanović, Darka M. and Perovanović, Jelena and Tucić, Nikola G",
year = "2011",
abstract = "We evaluated the degree of reproductive isolation between laboratory populations of the seed beetle (Acanthoscelides obtectus) selected to reproduce early (E) or late (L) in life, where different levels of sexual activity and sexual discrimination have been detected. We found a significant level of behavioral isolation among populations within the E selection regime in which beetles showed enhanced early-life fitness traits and low sexual activity. In contrast, substantially higher levels of sexual activity and an indiscriminate mating system inhibited rather than promoted pre-zygotic isolation between the L populations. Our results indicate that the study of sexual activity levels may be crucial for understanding the first steps in the pre-zygotic isolation among allopatric populations subjected to uniform selection.",
journal = "Ethology",
title = "Sexual Activity and Reproductive Isolation Between Age-specific Selected Populations of Seed Beetle",
number = "9",
volume = "117",
pages = "821",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1271"
}

Stojković, B., Šešlija-Jovanović, D. M., Perovanović, J.,& Tucić, N. G.. (2011). Sexual Activity and Reproductive Isolation Between Age-specific Selected Populations of Seed Beetle. in Ethology, 117(9).
https://hdl.handle.net/21.15107/rcub_ibiss_1271

Stojković B, Šešlija-Jovanović DM, Perovanović J, Tucić NG. Sexual Activity and Reproductive Isolation Between Age-specific Selected Populations of Seed Beetle. in Ethology. 2011;117(9):null-821.
https://hdl.handle.net/21.15107/rcub_ibiss_1271 .

Stojković, Biljana, Šešlija-Jovanović, Darka M., Perovanović, Jelena, Tucić, Nikola G, "Sexual Activity and Reproductive Isolation Between Age-specific Selected Populations of Seed Beetle" in Ethology, 117, no. 9 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1271 .

TY  - JOUR
AU  - Vuleta, Ana
AU  - Manitašević Jovanović, Sanja
AU  - Šešlija Jovanović, Darka
AU  - Tucić, Branka
PY  - 2010
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5895
AB  - Plants in their natural habitats frequently cope with a multitude of abiotic stresses, such as high light intensity, extreme temperatures and water deficit, which often co-occur during periods of drought, especially in semi-arid and arid regions. Exposure of plants to stressful environmental conditions usually induce overproduction of reactive oxygen species (ROS) that, as highly toxic derivatives of O2, can assault all cell macromolecules, leading to the disruption of cellular homeostasis and, consequently, the uncoupling of major metabolic processes, the photosynthesis and photorespiration. In order to minimize ROS-mediated cellular damage, plants have evolved highly efficient antioxidative defense systems that include both enzymatic and non-enzymatic components. Since abiotic stress can also operate as a strong evolutionary force that shapes adaptations in natural plant populations, the aim of this study was to examine the seasonal variation patterns of two distinct antioxidative systems, ROS-scavenging enzymes and anthocyanin pigments, in the leaf tissue of a steppe plant, Iris pumila, as expressed under contrasting light conditions that the species regularly experiences in the wild.
PB  - Oxford, UK: Oxford University Press
T2  - Journal of Plant Ecology
T1  - Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L.
IS  - 1
VL  - 3
DO  - 10.1093/jpe/rtp019
SP  - 59
EP  - 69
ER  - 

@article{
author = "Vuleta, Ana and Manitašević Jovanović, Sanja and Šešlija Jovanović, Darka and Tucić, Branka",
year = "2010",
abstract = "Plants in their natural habitats frequently cope with a multitude of abiotic stresses, such as high light intensity, extreme temperatures and water deficit, which often co-occur during periods of drought, especially in semi-arid and arid regions. Exposure of plants to stressful environmental conditions usually induce overproduction of reactive oxygen species (ROS) that, as highly toxic derivatives of O2, can assault all cell macromolecules, leading to the disruption of cellular homeostasis and, consequently, the uncoupling of major metabolic processes, the photosynthesis and photorespiration. In order to minimize ROS-mediated cellular damage, plants have evolved highly efficient antioxidative defense systems that include both enzymatic and non-enzymatic components. Since abiotic stress can also operate as a strong evolutionary force that shapes adaptations in natural plant populations, the aim of this study was to examine the seasonal variation patterns of two distinct antioxidative systems, ROS-scavenging enzymes and anthocyanin pigments, in the leaf tissue of a steppe plant, Iris pumila, as expressed under contrasting light conditions that the species regularly experiences in the wild.",
publisher = "Oxford, UK: Oxford University Press",
journal = "Journal of Plant Ecology",
title = "Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L.",
number = "1",
volume = "3",
doi = "10.1093/jpe/rtp019",
pages = "59-69"
}

Vuleta, A., Manitašević Jovanović, S., Šešlija Jovanović, D.,& Tucić, B.. (2010). Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L.. in Journal of Plant Ecology
Oxford, UK: Oxford University Press., 3(1), 59-69.
https://doi.org/10.1093/jpe/rtp019

Vuleta A, Manitašević Jovanović S, Šešlija Jovanović D, Tucić B. Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L.. in Journal of Plant Ecology. 2010;3(1):59-69.
doi:10.1093/jpe/rtp019 .

Vuleta, Ana, Manitašević Jovanović, Sanja, Šešlija Jovanović, Darka, Tucić, Branka, "Seasonal dynamics of foliar antioxidative enzymes and total anthocyanins in natural populations of Iris pumila L." in Journal of Plant Ecology, 3, no. 1 (2010):59-69,
https://doi.org/10.1093/jpe/rtp019 . .

TY  - JOUR
AU  - Stojković, Biljana
AU  - Šešlija-Jovanović, Darka M.
AU  - Tucić, Branka
AU  - Tucić, Nikola G
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1333
AB  - The level of homosexual behaviour is evaluated in one laboratory population of seed beetle and derived lines selected to reproduce early (E) or late in life (L), where inadvertent selection for either low or high heterosexual activity has been detected. The magnitudes of homosexual interaction, measured as chasing and mounting individuals of the same sex, are estimated over different age classes. These magnitudes are correlated with previously observed levels and patterns of age-specific variation of heterosexual activity of both sexes in the E and L experimental lines. The results obtained support the perception error hypothesis proposing that a low degree of sexual discrimination is genetically correlated with high sexual activity. The fitness costs of the same-sex interactions are tested by assessing their effects on longevity. In both sexes, the longevities of homosexual pairs are reduced relative to individually-housed virgin beetles in both the E and L lines, although homosexual interactions have a more pronounced effect on male survival than on female survival. Although the results obtained suggest that the longevity cost of homosexual interactions can be substantial, this cost is much smaller than the cost of heterosexual interactions.
T2  - Physiological Entomology
T1  - Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus
IS  - 4
VL  - 35
EP  - 316
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1333
ER  - 

@article{
author = "Stojković, Biljana and Šešlija-Jovanović, Darka M. and Tucić, Branka and Tucić, Nikola G",
year = "2010",
abstract = "The level of homosexual behaviour is evaluated in one laboratory population of seed beetle and derived lines selected to reproduce early (E) or late in life (L), where inadvertent selection for either low or high heterosexual activity has been detected. The magnitudes of homosexual interaction, measured as chasing and mounting individuals of the same sex, are estimated over different age classes. These magnitudes are correlated with previously observed levels and patterns of age-specific variation of heterosexual activity of both sexes in the E and L experimental lines. The results obtained support the perception error hypothesis proposing that a low degree of sexual discrimination is genetically correlated with high sexual activity. The fitness costs of the same-sex interactions are tested by assessing their effects on longevity. In both sexes, the longevities of homosexual pairs are reduced relative to individually-housed virgin beetles in both the E and L lines, although homosexual interactions have a more pronounced effect on male survival than on female survival. Although the results obtained suggest that the longevity cost of homosexual interactions can be substantial, this cost is much smaller than the cost of heterosexual interactions.",
journal = "Physiological Entomology",
title = "Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus",
number = "4",
volume = "35",
pages = "316",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1333"
}

Stojković, B., Šešlija-Jovanović, D. M., Tucić, B.,& Tucić, N. G.. (2010). Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus. in Physiological Entomology, 35(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1333

Stojković B, Šešlija-Jovanović DM, Tucić B, Tucić NG. Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus. in Physiological Entomology. 2010;35(4):null-316.
https://hdl.handle.net/21.15107/rcub_ibiss_1333 .

Stojković, Biljana, Šešlija-Jovanović, Darka M., Tucić, Branka, Tucić, Nikola G, "Homosexual behaviour and its longevity cost in females and males of the seed beetle Acanthoscelides obtectus" in Physiological Entomology, 35, no. 4 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1333 .

TY  - JOUR
AU  - Šešlija, Darka M.
AU  - Stojković, Biljana
AU  - Tucić, Branka
AU  - Tucić, Nikola G
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1131
AB  - In the present study the egg dumping behaviour in short (E)- and long (L)-lived lines of the seed beetle Acanthoscelides obtectus was analyzed. Females of the short-lived E line exhibited substantially higher egg dumping than long-lived L line females. We hypothesize that, since cessation of egg dumping enhances longevity, non-dumping females were selectively favoured in the L regime. Our study also produced evidence that the selection regime affected the male's ability to influence female egg-dumping behaviour. The females mated to males from the lines that were selected for extended longevity and of which the females exhibited little egg-dumping dumped fewer eggs. We suggest that in the L selection regime, where offspring produced at the end of the females' reproductive period were recruited to the next generation, selection operated against those males that stimulated female oviposition in the absence of seeds. This is the first study to provide evidence that selection for long-lived insects results in the reduced potency of male seminal products to stimulate female oviposition.
T2  - European Journal of Entomology
T1  - Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction
IS  - 4
VL  - 106
SP  - 255
EP  - 563
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1131
ER  - 

@article{
author = "Šešlija, Darka M. and Stojković, Biljana and Tucić, Branka and Tucić, Nikola G",
year = "2009",
abstract = "In the present study the egg dumping behaviour in short (E)- and long (L)-lived lines of the seed beetle Acanthoscelides obtectus was analyzed. Females of the short-lived E line exhibited substantially higher egg dumping than long-lived L line females. We hypothesize that, since cessation of egg dumping enhances longevity, non-dumping females were selectively favoured in the L regime. Our study also produced evidence that the selection regime affected the male's ability to influence female egg-dumping behaviour. The females mated to males from the lines that were selected for extended longevity and of which the females exhibited little egg-dumping dumped fewer eggs. We suggest that in the L selection regime, where offspring produced at the end of the females' reproductive period were recruited to the next generation, selection operated against those males that stimulated female oviposition in the absence of seeds. This is the first study to provide evidence that selection for long-lived insects results in the reduced potency of male seminal products to stimulate female oviposition.",
journal = "European Journal of Entomology",
title = "Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction",
number = "4",
volume = "106",
pages = "255-563",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1131"
}

Šešlija, D. M., Stojković, B., Tucić, B.,& Tucić, N. G.. (2009). Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction. in European Journal of Entomology, 106(4), 255-563.
https://hdl.handle.net/21.15107/rcub_ibiss_1131

Šešlija DM, Stojković B, Tucić B, Tucić NG. Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction. in European Journal of Entomology. 2009;106(4):255-563.
https://hdl.handle.net/21.15107/rcub_ibiss_1131 .

Šešlija, Darka M., Stojković, Biljana, Tucić, Branka, Tucić, Nikola G, "Egg-dumping behaviour in the seed beetle Acanthoscelides obtectus (Coleoptera: Chrysomelidae: Bruchinae) selected for early and late reproduction" in European Journal of Entomology, 106, no. 4 (2009):255-563,
https://hdl.handle.net/21.15107/rcub_ibiss_1131 .

TY  - JOUR
AU  - Šešlija, Darka M.
AU  - Tucić, Nikola G
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1554
AB  - We studied the genetic architecture of the differences in the longevity between lines selected for postponed senescence and a control population of the seed beetle Acanthoscelides obtectus maintained on two hosts. By using lines with increased longevity, which were obtained by selection on natural variation in longevity, we showed that the genetic architecture of seed beetle longevity is complex, with sex-specific effects and variation attributable to many interacting genes, whose expression depend on the host on which the beetles were reared. The nonadditive genetic effects were more strongly expressed when reared on chickpeas, a novel host, than on beans. Outbreeding depression, with respect to longevity, was a consequence of both the intrinsic effect of interactions between genes from different parental sources ( disruption of coadapted gene complexes) and the genotype x host interaction ( loss of local adaptation).
T2  - European Journal of Entomology
T1  - The genetic architecture of extended life span in the seed beetle Acanthoscelides obtectus (Coleoptera: Bruchidae)
IS  - 4
VL  - 105
EP  - 560
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1554
ER  - 

@article{
author = "Šešlija, Darka M. and Tucić, Nikola G",
year = "2008",
abstract = "We studied the genetic architecture of the differences in the longevity between lines selected for postponed senescence and a control population of the seed beetle Acanthoscelides obtectus maintained on two hosts. By using lines with increased longevity, which were obtained by selection on natural variation in longevity, we showed that the genetic architecture of seed beetle longevity is complex, with sex-specific effects and variation attributable to many interacting genes, whose expression depend on the host on which the beetles were reared. The nonadditive genetic effects were more strongly expressed when reared on chickpeas, a novel host, than on beans. Outbreeding depression, with respect to longevity, was a consequence of both the intrinsic effect of interactions between genes from different parental sources ( disruption of coadapted gene complexes) and the genotype x host interaction ( loss of local adaptation).",
journal = "European Journal of Entomology",
title = "The genetic architecture of extended life span in the seed beetle Acanthoscelides obtectus (Coleoptera: Bruchidae)",
number = "4",
volume = "105",
pages = "560",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1554"
}

Šešlija, D. M.,& Tucić, N. G.. (2008). The genetic architecture of extended life span in the seed beetle Acanthoscelides obtectus (Coleoptera: Bruchidae). in European Journal of Entomology, 105(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1554

Šešlija DM, Tucić NG. The genetic architecture of extended life span in the seed beetle Acanthoscelides obtectus (Coleoptera: Bruchidae). in European Journal of Entomology. 2008;105(4):null-560.
https://hdl.handle.net/21.15107/rcub_ibiss_1554 .

Šešlija, Darka M., Tucić, Nikola G, "The genetic architecture of extended life span in the seed beetle Acanthoscelides obtectus (Coleoptera: Bruchidae)" in European Journal of Entomology, 105, no. 4 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1554 .

TY  - JOUR
AU  - Šešlija, Darka M.
AU  - Marecko, Ilona
AU  - Tucić, Nikola G
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1508
AB  - Although the reasons why organisms age and die are generally well understood, it has recently been suggested that an optimal life span has evolved not only as the result of trade-offs between reproductive performances early and late in life, but also that a balance between the costs and benefits of the number of mating has also played an important role in the evolution of ageing in both sexes. By using four seed beetle (Acanthoscelides obtectus) lines selected for different life history traits, but which have also inadvertently created monoandrous and polyandrous conditions, we showed that males evolved to affect the mortality patterns of females in a way consistent to the postmating sexual selection generated by sexually antagonistic co-evolution theory. Monoandrous males, irrespectively of body weight and other life history traits specific to their lines, evolved to increase the longevity of control females kept under starvation and suppressed fecundity, compared with males that originated in the lines with effectively polyandrous conditions. When females were allowed to lay eggs, the effects of males from different lines and mating type history on the senescence of females were substantially weaker. We found that males in the line that was evolved to decelerate senescence and polyandrous conditions stimulate the earlier onset of females' oviposition, relative to males stemmed from the line with accelerated senescence and monoandrous conditions. This fact may explain the absence of difference in the mean longevities between the control females mated to these males and highlight the importance of sexual selection in the evolution of ageing.
T2  - Journal of Zoological Systematics and Evolutionary Research
T1  - Sexual selection and senescence: do seed beetle males (Acanthoscelides obtectus, Bruchidae, Coleoptera) shape the longevity of their mates?
IS  - 4
VL  - 46
EP  - 330
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1508
ER  - 

@article{
author = "Šešlija, Darka M. and Marecko, Ilona and Tucić, Nikola G",
year = "2008",
abstract = "Although the reasons why organisms age and die are generally well understood, it has recently been suggested that an optimal life span has evolved not only as the result of trade-offs between reproductive performances early and late in life, but also that a balance between the costs and benefits of the number of mating has also played an important role in the evolution of ageing in both sexes. By using four seed beetle (Acanthoscelides obtectus) lines selected for different life history traits, but which have also inadvertently created monoandrous and polyandrous conditions, we showed that males evolved to affect the mortality patterns of females in a way consistent to the postmating sexual selection generated by sexually antagonistic co-evolution theory. Monoandrous males, irrespectively of body weight and other life history traits specific to their lines, evolved to increase the longevity of control females kept under starvation and suppressed fecundity, compared with males that originated in the lines with effectively polyandrous conditions. When females were allowed to lay eggs, the effects of males from different lines and mating type history on the senescence of females were substantially weaker. We found that males in the line that was evolved to decelerate senescence and polyandrous conditions stimulate the earlier onset of females' oviposition, relative to males stemmed from the line with accelerated senescence and monoandrous conditions. This fact may explain the absence of difference in the mean longevities between the control females mated to these males and highlight the importance of sexual selection in the evolution of ageing.",
journal = "Journal of Zoological Systematics and Evolutionary Research",
title = "Sexual selection and senescence: do seed beetle males (Acanthoscelides obtectus, Bruchidae, Coleoptera) shape the longevity of their mates?",
number = "4",
volume = "46",
pages = "330",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1508"
}

Šešlija, D. M., Marecko, I.,& Tucić, N. G.. (2008). Sexual selection and senescence: do seed beetle males (Acanthoscelides obtectus, Bruchidae, Coleoptera) shape the longevity of their mates?. in Journal of Zoological Systematics and Evolutionary Research, 46(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1508

Šešlija DM, Marecko I, Tucić NG. Sexual selection and senescence: do seed beetle males (Acanthoscelides obtectus, Bruchidae, Coleoptera) shape the longevity of their mates?. in Journal of Zoological Systematics and Evolutionary Research. 2008;46(4):null-330.
https://hdl.handle.net/21.15107/rcub_ibiss_1508 .

Šešlija, Darka M., Marecko, Ilona, Tucić, Nikola G, "Sexual selection and senescence: do seed beetle males (Acanthoscelides obtectus, Bruchidae, Coleoptera) shape the longevity of their mates?" in Journal of Zoological Systematics and Evolutionary Research, 46, no. 4 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1508 .

TY  - JOUR
AU  - Tucić, Nikola G
AU  - Šešlija, Darka M.
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1604
AB  - We investigated the additive, dominance and epistatic genetic effects underlying differentiation in oviposition preference between two populations of the seed beetle Acanthoscelides obtectus evolved in the laboratory for 102 generations on bean and chickpea seeds. We reared and tested females on each of two host legumes. The populations differed in mean oviposition preference; the preference for chickpea was stronger in population reared on the chickpea ( C) than in population maintained on common bean ( P). Observations in the parental populations indicated that females tend to prefer ovipositioning their eggs on the seeds they have already experienced. The patterns of the means in each of the parental populations and 12 types of hybrids (two F-1, two F-2 and eight backcrosses) indicated that population differences in oviposition preference from both rearing hosts could be explained by nonadditive genetic effects. Statistically detectable additive and dominance genetic effects were observed in the most parsimonious model only when females were reared on the chickpea. The most parsimonious models on both rearing hosts suggested a contribution of negative additive x additive epistasis to the divergence of oviposition preference between the P and C populations. This indicates a positive effect of epistasis on the performance of the second generations of hybrids.
T2  - Heredity
T1  - Genetic architecture of differences in oviposition preference between ancestral and derived populations of the seed beetle Acanthoscelides obtectus
IS  - 5
VL  - 98
EP  - 273
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1604
ER  - 

@article{
author = "Tucić, Nikola G and Šešlija, Darka M.",
year = "2007",
abstract = "We investigated the additive, dominance and epistatic genetic effects underlying differentiation in oviposition preference between two populations of the seed beetle Acanthoscelides obtectus evolved in the laboratory for 102 generations on bean and chickpea seeds. We reared and tested females on each of two host legumes. The populations differed in mean oviposition preference; the preference for chickpea was stronger in population reared on the chickpea ( C) than in population maintained on common bean ( P). Observations in the parental populations indicated that females tend to prefer ovipositioning their eggs on the seeds they have already experienced. The patterns of the means in each of the parental populations and 12 types of hybrids (two F-1, two F-2 and eight backcrosses) indicated that population differences in oviposition preference from both rearing hosts could be explained by nonadditive genetic effects. Statistically detectable additive and dominance genetic effects were observed in the most parsimonious model only when females were reared on the chickpea. The most parsimonious models on both rearing hosts suggested a contribution of negative additive x additive epistasis to the divergence of oviposition preference between the P and C populations. This indicates a positive effect of epistasis on the performance of the second generations of hybrids.",
journal = "Heredity",
title = "Genetic architecture of differences in oviposition preference between ancestral and derived populations of the seed beetle Acanthoscelides obtectus",
number = "5",
volume = "98",
pages = "273",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1604"
}

Tucić, N. G.,& Šešlija, D. M.. (2007). Genetic architecture of differences in oviposition preference between ancestral and derived populations of the seed beetle Acanthoscelides obtectus. in Heredity, 98(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1604

Tucić NG, Šešlija DM. Genetic architecture of differences in oviposition preference between ancestral and derived populations of the seed beetle Acanthoscelides obtectus. in Heredity. 2007;98(5):null-273.
https://hdl.handle.net/21.15107/rcub_ibiss_1604 .

Tucić, Nikola G, Šešlija, Darka M., "Genetic architecture of differences in oviposition preference between ancestral and derived populations of the seed beetle Acanthoscelides obtectus" in Heredity, 98, no. 5 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1604 .