TY  - CONF
AU  - Mandić, Marija R.
AU  - Oalđe, Mariana M.
AU  - Lunić, Tanja M.
AU  - Sabovljević, Aneta D.
AU  - Sabovljević, Marko S.
AU  - Gašić, Uroš
AU  - Duletić Laušević, Sonja N.
AU  - Božić, Bojan D.
AU  - Božić Nedeljković, Biljana D.
PY  - 2021
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4881
AB  - ‚In this study, we aimed to examine the immunomodulatory potential (anti‐neuroinflammatory/neuroprotective and antitumor activities) of various seasonal aspects (spring, summer,
autumn) of moss Hypnum cupressiforme extracts. Moss extracts have been obtained using solvents of different polarities and their mixtures applying Soxhlet extractor. Activities
of the extracts were tested on cell lines MRC‐5, BV2, SH‐SY5Y, HCT‐116, and MDA‐MB‐231, for potential anti‐neuroinflammatory and antitumor activities. The impact of extracts on
cell viability/metabolic activity was measured by MTT assay, while the influence on the production of ROS and NO was determined using NBT and Griess assays, respectively. The
biocompatibility of extracts was confirmed using MRC‐5 cells. Extracts of moss from spring season have shown significant antiproliferative activity against MDA‐MB‐231 cell line
(inhibition rate ~50%), as well as anti‐neuroinflammatory activity. Furthermore, the effects of seasonal variation (spring, summer, autumn) on the immunomodulatory potential of
ethyl‐acetate extract were examined. Summer aspect of moss has led to the greatest reduction of ROS production, while autumn aspect had the greatest impact on the reduction of NO
production by LPS‐activated BV2 cells. Additionally, the supernatant‐transfer model proved that treatment with moss extracts from summer and autumn seasons of LPS‐activated BV2
cells exhibit neuroprotective activity towards SH‐SY5Y neurons. The obtained data suggest that extracts from moss H. cupressiforme possess significant immunomodulatory potential
evaluated in vitro, which may be useful in conditions such as Alzheimer’s disease, Parkinson’s disease, and breast cancer. In terms of anti‐neuroinflammatory/neuroprotective activity,
summer and autumn were characterized as the most favorable seasons.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons
DO  - 10.1002/eji.202170200
SP  - 296
ER  - 

@conference{
author = "Mandić, Marija R. and Oalđe, Mariana M. and Lunić, Tanja M. and Sabovljević, Aneta D. and Sabovljević, Marko S. and Gašić, Uroš and Duletić Laušević, Sonja N. and Božić, Bojan D. and Božić Nedeljković, Biljana D.",
year = "2021",
abstract = "‚In this study, we aimed to examine the immunomodulatory potential (anti‐neuroinflammatory/neuroprotective and antitumor activities) of various seasonal aspects (spring, summer,
autumn) of moss Hypnum cupressiforme extracts. Moss extracts have been obtained using solvents of different polarities and their mixtures applying Soxhlet extractor. Activities
of the extracts were tested on cell lines MRC‐5, BV2, SH‐SY5Y, HCT‐116, and MDA‐MB‐231, for potential anti‐neuroinflammatory and antitumor activities. The impact of extracts on
cell viability/metabolic activity was measured by MTT assay, while the influence on the production of ROS and NO was determined using NBT and Griess assays, respectively. The
biocompatibility of extracts was confirmed using MRC‐5 cells. Extracts of moss from spring season have shown significant antiproliferative activity against MDA‐MB‐231 cell line
(inhibition rate ~50%), as well as anti‐neuroinflammatory activity. Furthermore, the effects of seasonal variation (spring, summer, autumn) on the immunomodulatory potential of
ethyl‐acetate extract were examined. Summer aspect of moss has led to the greatest reduction of ROS production, while autumn aspect had the greatest impact on the reduction of NO
production by LPS‐activated BV2 cells. Additionally, the supernatant‐transfer model proved that treatment with moss extracts from summer and autumn seasons of LPS‐activated BV2
cells exhibit neuroprotective activity towards SH‐SY5Y neurons. The obtained data suggest that extracts from moss H. cupressiforme possess significant immunomodulatory potential
evaluated in vitro, which may be useful in conditions such as Alzheimer’s disease, Parkinson’s disease, and breast cancer. In terms of anti‐neuroinflammatory/neuroprotective activity,
summer and autumn were characterized as the most favorable seasons.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons",
doi = "10.1002/eji.202170200",
pages = "296"
}

Mandić, M. R., Oalđe, M. M., Lunić, T. M., Sabovljević, A. D., Sabovljević, M. S., Gašić, U., Duletić Laušević, S. N., Božić, B. D.,& Božić Nedeljković, B. D.. (2021). Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 296.
https://doi.org/10.1002/eji.202170200

Mandić MR, Oalđe MM, Lunić TM, Sabovljević AD, Sabovljević MS, Gašić U, Duletić Laušević SN, Božić BD, Božić Nedeljković BD. Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;:296.
doi:10.1002/eji.202170200 .

Mandić, Marija R., Oalđe, Mariana M., Lunić, Tanja M., Sabovljević, Aneta D., Sabovljević, Marko S., Gašić, Uroš, Duletić Laušević, Sonja N., Božić, Bojan D., Božić Nedeljković, Biljana D., "Immunomodulatory properties of extracts of moss Hypnum cupressiforme from various seasons" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting (2021):296,
https://doi.org/10.1002/eji.202170200 . .

TY  - JOUR
AU  - Mandić, Marija R.
AU  - Oalđe, Mariana M.
AU  - Lunić, Tanja M.
AU  - Sabovljević, Aneta D.
AU  - Sabovljević, Marko S.
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja N.
AU  - Božić, Bojan Đ.
AU  - Božić Nedeljković, Biljana Dj.
AU  - Chen, Jen-Tsung
PY  - 2021
UR  - internal-pdf://Mandić et al. - 2021 - Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (H.pdf
UR  - https://dx.plos.org/10.1371/journal.pone.0246810
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4156
AB  - Bioactive compounds from natural sources are of great importance because of their potential pharmacological activity and tremendous structural diversity. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. have been examined, as well as their antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative potential. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical characterization of the extracts revealed the presence of phenolics and flavonoid compounds, together with triterpenoids as secondary metabolites of high biological activity. Significant antioxidant properties of all the extracts were exhibited using the β-carotene assay. The highest activities were found for water:ethanol extract (with the highest inhibition rate of 96%), but also significant inhibition was measured for ethanol and ethyl acetate extracts (80% and 70%, respectively). Confirmation of biocompatibility of investigated moss extracts has been performed using normal human fibroblast cell line, MRC-5. The H . ciliata extracts exhibited significant antiproliferative activity (~ 50%) against the MDA-MB-231 (human breast adenocarcinoma cell line), which has not previously been reported elsewhere. The Griess assay confirmed the potential anti-neuroinflammatory activity of the extracts, as significant effects in reducing NO production by LPS-stimulated BV2 (normal murine microglia cell line) was observed. This data is in line with noted antineurodegenerative potential measured by the inhibition of acetylcholinesterase (with the highest inhibition rate of 60% for ethyl acetate extract) and tyrosinase (with the highest inhibition rate of 70% for ethanol extract). Additionally, the H . ciliata extracts exhibited significant antidiabetic effect mediated by α-glucosidase inhibition (with the highest inhibition rate of 80% for ethyl acetate extract). The obtained data suggest the presence of immunomodulatory effects of the moss extracts in vitro , which allows the design of new experiments aimed at detecting and characterizing bioactive compounds of the extracts and additionally elucidate detailed mechanisms of their effects.
PB  - Public Library of Science (PLoS)
T2  - PLOS ONE
T1  - Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia
IS  - 2
VL  - 16
DO  - 10.1371/journal.pone.0246810
SP  - e0246810
ER  - 

@article{
author = "Mandić, Marija R. and Oalđe, Mariana M. and Lunić, Tanja M. and Sabovljević, Aneta D. and Sabovljević, Marko S. and Gašić, Uroš and Duletić-Laušević, Sonja N. and Božić, Bojan Đ. and Božić Nedeljković, Biljana Dj. and Chen, Jen-Tsung",
year = "2021",
abstract = "Bioactive compounds from natural sources are of great importance because of their potential pharmacological activity and tremendous structural diversity. In this study, the chemical composition of different moss extracts of Hedwigia ciliata P. Beauv. have been examined, as well as their antioxidant, antineurodegenerative/anti-neuroinflammatory, antidiabetic, and antiproliferative potential. The extracts were prepared by Soxhlet extractor using solvents of different polarity. Chemical characterization of the extracts revealed the presence of phenolics and flavonoid compounds, together with triterpenoids as secondary metabolites of high biological activity. Significant antioxidant properties of all the extracts were exhibited using the β-carotene assay. The highest activities were found for water:ethanol extract (with the highest inhibition rate of 96%), but also significant inhibition was measured for ethanol and ethyl acetate extracts (80% and 70%, respectively). Confirmation of biocompatibility of investigated moss extracts has been performed using normal human fibroblast cell line, MRC-5. The H . ciliata extracts exhibited significant antiproliferative activity (~ 50%) against the MDA-MB-231 (human breast adenocarcinoma cell line), which has not previously been reported elsewhere. The Griess assay confirmed the potential anti-neuroinflammatory activity of the extracts, as significant effects in reducing NO production by LPS-stimulated BV2 (normal murine microglia cell line) was observed. This data is in line with noted antineurodegenerative potential measured by the inhibition of acetylcholinesterase (with the highest inhibition rate of 60% for ethyl acetate extract) and tyrosinase (with the highest inhibition rate of 70% for ethanol extract). Additionally, the H . ciliata extracts exhibited significant antidiabetic effect mediated by α-glucosidase inhibition (with the highest inhibition rate of 80% for ethyl acetate extract). The obtained data suggest the presence of immunomodulatory effects of the moss extracts in vitro , which allows the design of new experiments aimed at detecting and characterizing bioactive compounds of the extracts and additionally elucidate detailed mechanisms of their effects.",
publisher = "Public Library of Science (PLoS)",
journal = "PLOS ONE",
title = "Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia",
number = "2",
volume = "16",
doi = "10.1371/journal.pone.0246810",
pages = "e0246810"
}

Mandić, M. R., Oalđe, M. M., Lunić, T. M., Sabovljević, A. D., Sabovljević, M. S., Gašić, U., Duletić-Laušević, S. N., Božić, B. Đ., Božić Nedeljković, B. Dj.,& Chen, J.. (2021). Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia. in PLOS ONE
Public Library of Science (PLoS)., 16(2), e0246810.
https://doi.org/10.1371/journal.pone.0246810

Mandić MR, Oalđe MM, Lunić TM, Sabovljević AD, Sabovljević MS, Gašić U, Duletić-Laušević SN, Božić BĐ, Božić Nedeljković BD, Chen J. Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia. in PLOS ONE. 2021;16(2):e0246810.
doi:10.1371/journal.pone.0246810 .

Mandić, Marija R., Oalđe, Mariana M., Lunić, Tanja M., Sabovljević, Aneta D., Sabovljević, Marko S., Gašić, Uroš, Duletić-Laušević, Sonja N., Božić, Bojan Đ., Božić Nedeljković, Biljana Dj., Chen, Jen-Tsung, "Chemical characterization and in vitro immunomodulatory effects of different extracts of moss Hedwigia ciliata (Hedw.) P. Beauv. from the Vršačke Planine Mts., Serbia" in PLOS ONE, 16, no. 2 (2021):e0246810,
https://doi.org/10.1371/journal.pone.0246810 . .