TY  - JOUR
AU  - Tubić, Ljiljana
AU  - Anackov, Goran
AU  - Milojević, Jelena
AU  - Ghalawenji, Nabil
AU  - Banjac, Nevena
AU  - Igić, Ružica
AU  - Zdravković-Korać, Snežana
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2198
AB  - This study assessed the shoot regeneration capacity of root-tips
   isolated from single seed-derived individual plants, obtained from a
   wild, open-pollinated micropopulation of shallot (Allium ascalonicum).
   Considerable variation was observed in the regeneration capacities of
   individual lines, ranging from 0.93 to 100 \%, and a mean bud number per
   root explant between 0.09 and 20.67. One line was found to be superior,
   and was chosen for protocol optimization, focusing on the 2,4-D/BA
   ratio, duration of the CI phase and light conditions. The application of
   the optimized protocol to other lines, selected for their variable
   regeneration capacities, enhanced the process of regeneration and
   shortened the time required to obtain healthy plantlets, even in
   inferior lines. However, highly responsive lines remained superior,
   indicating that differences at the individual level must not be
   overlooked. The conditions employed in this study may serve as a base to
   facilitate the application of molecular breeding methods in shallot.
T2  - Plant Cell Tissue and Organ Culture
T1  - High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure
IS  - 1
VL  - 118
DO  - 10.1007/s11240-014-0465-9
SP  - 101
EP  - 110
ER  - 

@article{
author = "Tubić, Ljiljana and Anackov, Goran and Milojević, Jelena and Ghalawenji, Nabil and Banjac, Nevena and Igić, Ružica and Zdravković-Korać, Snežana",
year = "2014",
abstract = "This study assessed the shoot regeneration capacity of root-tips
   isolated from single seed-derived individual plants, obtained from a
   wild, open-pollinated micropopulation of shallot (Allium ascalonicum).
   Considerable variation was observed in the regeneration capacities of
   individual lines, ranging from 0.93 to 100 \%, and a mean bud number per
   root explant between 0.09 and 20.67. One line was found to be superior,
   and was chosen for protocol optimization, focusing on the 2,4-D/BA
   ratio, duration of the CI phase and light conditions. The application of
   the optimized protocol to other lines, selected for their variable
   regeneration capacities, enhanced the process of regeneration and
   shortened the time required to obtain healthy plantlets, even in
   inferior lines. However, highly responsive lines remained superior,
   indicating that differences at the individual level must not be
   overlooked. The conditions employed in this study may serve as a base to
   facilitate the application of molecular breeding methods in shallot.",
journal = "Plant Cell Tissue and Organ Culture",
title = "High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure",
number = "1",
volume = "118",
doi = "10.1007/s11240-014-0465-9",
pages = "101-110"
}

Tubić, L., Anackov, G., Milojević, J., Ghalawenji, N., Banjac, N., Igić, R.,& Zdravković-Korać, S.. (2014). High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure. in Plant Cell Tissue and Organ Culture, 118(1), 101-110.
https://doi.org/10.1007/s11240-014-0465-9

Tubić L, Anackov G, Milojević J, Ghalawenji N, Banjac N, Igić R, Zdravković-Korać S. High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure. in Plant Cell Tissue and Organ Culture. 2014;118(1):101-110.
doi:10.1007/s11240-014-0465-9 .

Tubić, Ljiljana, Anackov, Goran, Milojević, Jelena, Ghalawenji, Nabil, Banjac, Nevena, Igić, Ružica, Zdravković-Korać, Snežana, "High variability in the tissue culture response of root-tips of Allium
 ascalonicum individuals and optimization of the regeneration procedure" in Plant Cell Tissue and Organ Culture, 118, no. 1 (2014):101-110,
https://doi.org/10.1007/s11240-014-0465-9 . .