TY  - JOUR
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
AU  - Miler, Marko
AU  - Stanković, Sanja
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S094096022100162X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4499
AB  - Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.
PB  - Elsevier GmbH
T2  - Annals of Anatomy - Anatomischer Anzeiger
T1  - Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause
VL  - 239
DO  - 10.1016/j.aanat.2021.151836
SP  - 151836
ER  - 

@article{
author = "Živanović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir and Miler, Marko and Stanković, Sanja and Milošević, Verica and Filipović, Branko",
year = "2022",
abstract = "Soy isoflavone genistein interplays with numerous physiological or pathophysiological processes during ageing. However, its protective role and underlying mechanisms of action in the regulation of calcium (Ca2+) and phosphate (Pi) homeostasis in an animal model of the andropause are yet to be fully clarified. Wistar male rats (16-month-old) were divided into sham-operated, orchidectomized, orchidectomized estradiol-treated (0.625 mg/kg b.m./day) and orchidectomized genistein-treated (30 mg/kg b.m./day) groups. Treatments were administered subcutaneously for 3 weeks, while the controls received vehicle alone. Estradiol treatment increased the expression level of fibroblast growth factor receptor (FGFR) and parathyroid hormone 1 receptor (PTH1R), and activated mitogen – activated protein kinase kinase 1/2 (MEK 1/2) signaling pathway in the kidneys. Genistein application induced a prominent gene and protein expression of Klotho and downregulated the expression of FGFR and PTH1R in the kidney of andropausal rats. Activation of protein kinase B (Akt) signalling pathway was observed, while MEK 1/2 signaling pathway wasn't altered after genistein treatment. The increase of 25 (OH) vitamin D in the serum and decrease in Ca2+ urine content was observed after genistein application. Our findings strongly suggest genistein as a potent biocompound with beneficial effects on the regulation of Ca2+ and Pi homeostasis, especially during aging process when the balance of mineral metabolism is impaired. These novel data provide closer insights into the physiological roles of genistein in the regulation of mineral homeostasis.",
publisher = "Elsevier GmbH",
journal = "Annals of Anatomy - Anatomischer Anzeiger",
title = "Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause",
volume = "239",
doi = "10.1016/j.aanat.2021.151836",
pages = "151836"
}

Živanović, J., Jarić, I., Ajdžanović, V., Miler, M., Stanković, S., Milošević, V.,& Filipović, B.. (2022). Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause. in Annals of Anatomy - Anatomischer Anzeiger
Elsevier GmbH., 239, 151836.
https://doi.org/10.1016/j.aanat.2021.151836

Živanović J, Jarić I, Ajdžanović V, Miler M, Stanković S, Milošević V, Filipović B. Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause. in Annals of Anatomy - Anatomischer Anzeiger. 2022;239:151836.
doi:10.1016/j.aanat.2021.151836 .

Živanović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, Miler, Marko, Stanković, Sanja, Milošević, Verica, Filipović, Branko, "Genistein regulates calcium and phosphate homeostasis without activation of MEK 1/2 signalling pathway in an animal model of the andropause" in Annals of Anatomy - Anatomischer Anzeiger, 239 (2022):151836,
https://doi.org/10.1016/j.aanat.2021.151836 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Milenković, Dragan
AU  - Jarić, Ivana
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2020
UR  - https://dx.doi.org/10.1021/acs.jafc.0c03079
UR  - https://radar.ibiss.bg.ac.rs/123456789/3860
AB  - A growing population of elderly people consume citrus flavanones, naringenin, and hesperetin in the form of fruits or juices. Flavanones are bioactives with potent antioxidant properties and have potential in slowing down the aging process. Because flavanones exert controversial effects on pituitary-thyroid functioning, our study on the old-aged rat model aimed to elucidate the mechanism by which naringenin and hesperetin affect this axis. Naringenin and hesperetin increased the Sirt1 mRNA level by 91 and 71% (p < 0.05), which was followed by increased Sirt1 expression by 20 and 15% (p < 0.05), respectively. Only naringenin decreased thyroid-stimulating hormone expression by 20% (p < 0.05). Thyroid peroxidase protein expression was upregulated after naringenin or hesperetin by 62 and 43% (p < 0.05), respectively. Naringenin lowered mRNA levels of Tpo, Sod1, Sod2, Cat, and Nrf2 by 50, 32, 45, 35, and 42% (p < 0.05), respectively, and increased Gpx by 54% (p < 0.05), while hesperetin decreased Sod1 and Sod2 mRNA levels by 46 and 55% (p < 0.05), respectively. Naringenin increased the protein expressions of Nrf2 and SOD2 by 58 and 50% (p < 0.05), respectively, and decreased SOD1 expression by 48% (p < 0.05), while hesperetin protein decreased expressions of SOD1 and Nrf2 by 63 and 32% (p < 0.05), respectively. Altogether, our findings suggest that citrus flavanones contribute to restoring the impaired thyroid functioning in the old-aged rats.
PB  - NLM (Medline)
T2  - Journal of Agricultural and Food Chemistry
T1  - Citrus Flavanones Upregulate Thyrotroph Sirt1 and Differently Affect Thyroid Nrf2 Expressions in Old-Aged Wistar Rats
IS  - 31
VL  - 68
DO  - 10.1021/acs.jafc.0c03079
SP  - 8242
EP  - 8254
ER  - 

@article{
author = "Miler, Marko and Živanović, Jasmina and Ajdžanović, Vladimir and Milenković, Dragan and Jarić, Ivana and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2020",
abstract = "A growing population of elderly people consume citrus flavanones, naringenin, and hesperetin in the form of fruits or juices. Flavanones are bioactives with potent antioxidant properties and have potential in slowing down the aging process. Because flavanones exert controversial effects on pituitary-thyroid functioning, our study on the old-aged rat model aimed to elucidate the mechanism by which naringenin and hesperetin affect this axis. Naringenin and hesperetin increased the Sirt1 mRNA level by 91 and 71% (p < 0.05), which was followed by increased Sirt1 expression by 20 and 15% (p < 0.05), respectively. Only naringenin decreased thyroid-stimulating hormone expression by 20% (p < 0.05). Thyroid peroxidase protein expression was upregulated after naringenin or hesperetin by 62 and 43% (p < 0.05), respectively. Naringenin lowered mRNA levels of Tpo, Sod1, Sod2, Cat, and Nrf2 by 50, 32, 45, 35, and 42% (p < 0.05), respectively, and increased Gpx by 54% (p < 0.05), while hesperetin decreased Sod1 and Sod2 mRNA levels by 46 and 55% (p < 0.05), respectively. Naringenin increased the protein expressions of Nrf2 and SOD2 by 58 and 50% (p < 0.05), respectively, and decreased SOD1 expression by 48% (p < 0.05), while hesperetin protein decreased expressions of SOD1 and Nrf2 by 63 and 32% (p < 0.05), respectively. Altogether, our findings suggest that citrus flavanones contribute to restoring the impaired thyroid functioning in the old-aged rats.",
publisher = "NLM (Medline)",
journal = "Journal of Agricultural and Food Chemistry",
title = "Citrus Flavanones Upregulate Thyrotroph Sirt1 and Differently Affect Thyroid Nrf2 Expressions in Old-Aged Wistar Rats",
number = "31",
volume = "68",
doi = "10.1021/acs.jafc.0c03079",
pages = "8242-8254"
}

Miler, M., Živanović, J., Ajdžanović, V., Milenković, D., Jarić, I., Šošić-Jurjević, B.,& Milošević, V.. (2020). Citrus Flavanones Upregulate Thyrotroph Sirt1 and Differently Affect Thyroid Nrf2 Expressions in Old-Aged Wistar Rats. in Journal of Agricultural and Food Chemistry
NLM (Medline)., 68(31), 8242-8254.
https://doi.org/10.1021/acs.jafc.0c03079

Miler M, Živanović J, Ajdžanović V, Milenković D, Jarić I, Šošić-Jurjević B, Milošević V. Citrus Flavanones Upregulate Thyrotroph Sirt1 and Differently Affect Thyroid Nrf2 Expressions in Old-Aged Wistar Rats. in Journal of Agricultural and Food Chemistry. 2020;68(31):8242-8254.
doi:10.1021/acs.jafc.0c03079 .

Miler, Marko, Živanović, Jasmina, Ajdžanović, Vladimir, Milenković, Dragan, Jarić, Ivana, Šošić-Jurjević, Branka , Milošević, Verica, "Citrus Flavanones Upregulate Thyrotroph Sirt1 and Differently Affect Thyroid Nrf2 Expressions in Old-Aged Wistar Rats" in Journal of Agricultural and Food Chemistry, 68, no. 31 (2020):8242-8254,
https://doi.org/10.1021/acs.jafc.0c03079 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Šošić-Jurjević, Branka
AU  - Potrebić, Milica
AU  - Milošević, Verica
PY  - 2019
UR  - http://online.journals.tubitak.gov.tr/openDoiPdf.htm?mKodu=vet-1812-39
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3402
AB  - Letter to the Editor
T2  - Turkish Journal of Veterinary and Animal Sciences
T1  - Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model
IS  - 3
VL  - 43
DO  - 10.3906/vet-1812-39
SP  - 423
EP  - 426
ER  - 

@article{
author = "Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Jarić, Ivana and Šošić-Jurjević, Branka and Potrebić, Milica and Milošević, Verica",
year = "2019",
abstract = "Letter to the Editor",
journal = "Turkish Journal of Veterinary and Animal Sciences",
title = "Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model",
number = "3",
volume = "43",
doi = "10.3906/vet-1812-39",
pages = "423-426"
}

Miler, M., Ajdžanović, V., Živanović, J., Jarić, I., Šošić-Jurjević, B., Potrebić, M.,& Milošević, V.. (2019). Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model. in Turkish Journal of Veterinary and Animal Sciences, 43(3), 423-426.
https://doi.org/10.3906/vet-1812-39

Miler M, Ajdžanović V, Živanović J, Jarić I, Šošić-Jurjević B, Potrebić M, Milošević V. Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model. in Turkish Journal of Veterinary and Animal Sciences. 2019;43(3):423-426.
doi:10.3906/vet-1812-39 .

Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Jarić, Ivana, Šošić-Jurjević, Branka, Potrebić, Milica, Milošević, Verica, "Old age-associated impairment of the rat liver antioxidant defense system: the basis for affirmation of the experimental model" in Turkish Journal of Veterinary and Animal Sciences, 43, no. 3 (2019):423-426,
https://doi.org/10.3906/vet-1812-39 . .

TY  - CONF
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2019
UR  - https://www.karger.com/Journal/Issue/278476
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3500
AB  - Objectives: Fruit-derived polyphenols can affect pituitary thyroid stimulating hormone (TSH) secretion, which is mediated by Sirt1 protein. Recently, we have shown that citrus polyphenol naringenin (NAR) increases serum TSH level and modify thyroid structure in terms of increased hormone biosynthesis. Keeping in mind that higher serum TSH level and elevated Sirt1 expression are tightly connected with longevity, we wanted to test whetherNAR administration affects pituitary TSH and Sirt1 immunofluorescent expression and changes thyroid ultrastructure in 24-month-old male Wistar rats.
Methods: NAR was administrated orally (15 mg/kg b.m.) during 4 weeks, while the control group received vehicle, sunflower oil. Quantitative analysis of labeled TSH and Sirt1 proteins in TSH cells considered the relative intensity of fluorescence (RIF) measurements, while ultrastructural analysis was performed in the thyroids. Results: Increased (p<0.05) Sirt1 RIF as well as Sirt1/TSH ratio were observed, while TSH content-reflecting RIF decreased (p<0.05), all after NAR treatment. In line with this effect of NAR, thyroids of the same group responded to direct TSH stimulation in comparison with the controls. Namely, ultrastructural analysis showed higher thyroid epithelium, well developed
rough endoplasmic reticulum and polymorphic lysosomes which were more abundant than in the control group. Some lysosomes were attached to colloid droplets, unequivocally indicating the gland secretory activity. Conclusion: Citrus flavanone NAR increases pituitary Sirt1/TSH expression ratio in old rats. This corresponds with decreased thyrotrope TSH content/increased secretion, while ultrastructure of the thyroids follows direct TSH stimulation. These results, for the first time, show potency of NAR to positively interfere with TSH secretion in old-aged rats, which may contribute to the healthy aging and longevity.
PB  - Basel: S. Karger AG
C3  - 42nd Annual Meeting of the European Thyroid Association Abstracts; 2019 Sep 7-10; Budapest, Hungary (European Thyroid Journal; Vol. 1; No. 1)
T1  - Naringenin increases pituitary Sirt1/TSH ratio and induces ultrastructural changes in the thyroids of old-aged male Wistar rats
DO  - 10.1159/000501012
SP  - 15
EP  - 16
ER  - 

@conference{
author = "Miler, Marko and Živanović, Jasmina and Ajdžanović, Vladimir and Jarić, Ivana and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2019",
abstract = "Objectives: Fruit-derived polyphenols can affect pituitary thyroid stimulating hormone (TSH) secretion, which is mediated by Sirt1 protein. Recently, we have shown that citrus polyphenol naringenin (NAR) increases serum TSH level and modify thyroid structure in terms of increased hormone biosynthesis. Keeping in mind that higher serum TSH level and elevated Sirt1 expression are tightly connected with longevity, we wanted to test whetherNAR administration affects pituitary TSH and Sirt1 immunofluorescent expression and changes thyroid ultrastructure in 24-month-old male Wistar rats.
Methods: NAR was administrated orally (15 mg/kg b.m.) during 4 weeks, while the control group received vehicle, sunflower oil. Quantitative analysis of labeled TSH and Sirt1 proteins in TSH cells considered the relative intensity of fluorescence (RIF) measurements, while ultrastructural analysis was performed in the thyroids. Results: Increased (p<0.05) Sirt1 RIF as well as Sirt1/TSH ratio were observed, while TSH content-reflecting RIF decreased (p<0.05), all after NAR treatment. In line with this effect of NAR, thyroids of the same group responded to direct TSH stimulation in comparison with the controls. Namely, ultrastructural analysis showed higher thyroid epithelium, well developed
rough endoplasmic reticulum and polymorphic lysosomes which were more abundant than in the control group. Some lysosomes were attached to colloid droplets, unequivocally indicating the gland secretory activity. Conclusion: Citrus flavanone NAR increases pituitary Sirt1/TSH expression ratio in old rats. This corresponds with decreased thyrotrope TSH content/increased secretion, while ultrastructure of the thyroids follows direct TSH stimulation. These results, for the first time, show potency of NAR to positively interfere with TSH secretion in old-aged rats, which may contribute to the healthy aging and longevity.",
publisher = "Basel: S. Karger AG",
journal = "42nd Annual Meeting of the European Thyroid Association Abstracts; 2019 Sep 7-10; Budapest, Hungary (European Thyroid Journal; Vol. 1; No. 1)",
title = "Naringenin increases pituitary Sirt1/TSH ratio and induces ultrastructural changes in the thyroids of old-aged male Wistar rats",
doi = "10.1159/000501012",
pages = "15-16"
}

Miler, M., Živanović, J., Ajdžanović, V., Jarić, I., Šošić-Jurjević, B.,& Milošević, V.. (2019). Naringenin increases pituitary Sirt1/TSH ratio and induces ultrastructural changes in the thyroids of old-aged male Wistar rats. in 42nd Annual Meeting of the European Thyroid Association Abstracts; 2019 Sep 7-10; Budapest, Hungary (European Thyroid Journal; Vol. 1; No. 1)
Basel: S. Karger AG., 15-16.
https://doi.org/10.1159/000501012

Miler M, Živanović J, Ajdžanović V, Jarić I, Šošić-Jurjević B, Milošević V. Naringenin increases pituitary Sirt1/TSH ratio and induces ultrastructural changes in the thyroids of old-aged male Wistar rats. in 42nd Annual Meeting of the European Thyroid Association Abstracts; 2019 Sep 7-10; Budapest, Hungary (European Thyroid Journal; Vol. 1; No. 1). 2019;:15-16.
doi:10.1159/000501012 .

Miler, Marko, Živanović, Jasmina, Ajdžanović, Vladimir, Jarić, Ivana, Šošić-Jurjević, Branka , Milošević, Verica, "Naringenin increases pituitary Sirt1/TSH ratio and induces ultrastructural changes in the thyroids of old-aged male Wistar rats" in 42nd Annual Meeting of the European Thyroid Association Abstracts; 2019 Sep 7-10; Budapest, Hungary (European Thyroid Journal; Vol. 1; No. 1) (2019):15-16,
https://doi.org/10.1159/000501012 . .

TY  - JOUR
AU  - Živanović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
AU  - Mojić, Marija
AU  - Miler, Marko
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
AU  - Filipović, Branko
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0940960218301080?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3150
AB  - In a rat model of the andropause we aimed to examine the influence of daidzein, soy isoflavone, on the structure and function of parathyroid glands (PTG) and the expression levels of some of the crucial regulators of Ca2+ and Pi homeostasis in the kidney, and to compare these effects with the effects of estradiol, serving as a positive control. Middle-aged (16-month-old) male Wistar rats were divided into the following groups: sham-operated (SO), orchidectomized (Orx), orchidectomized and estradiol-treated (Orx+E; 0.625mg/kg b.w./day, s.c.) as well as orchidectomized and daidzein-treated (Orx+D; 30mg/kg b.w./day, s.c.) group. Every treated group had a corresponding control group. PTH serum concentration was decreased in Orx+E and Orx+D groups by 10% and 21% (p<0.05) respectively, in comparison with the Orx. PTG volume was decreased in Orx+E group by 16% (p<0.05), when compared to the Orx. In Orx+E group expression of NaPi 2a was lower (p<0.05), while NaPi 2a abundance in Orx+D animals was increased (p<0.05), when compared to Orx. Expression of PTH1R was increased (p<0.05) in Orx+E group, while in Orx+D animals the same parameter was decreased (p<0.05), in comparison with Orx. Klotho expression was elevated (p<0.05) in Orx+D rats, in regard to Orx. Orx+D induced reduction in Ca2+/creatinine and Pi/creatinine ratio in urine by 32% and 16% (p<0.05) respectively, in comparison with Orx. In conclusion, presented results indicate the more coherent beneficial effects of daidzein compared to estradiol, on disturbed Ca2+ and Pi homeostasis, and presumably on bone health, in the aging male rats.
T2  - Annals of Anatomy = Anatomischer Anzeiger
T1  - Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause.
VL  - 221
DO  - 10.1016/j.aanat.2018.08.001
SP  - 27
EP  - 37
ER  - 

@article{
author = "Živanović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir and Mojić, Marija and Miler, Marko and Šošić-Jurjević, Branka  and Milošević, Verica and Filipović, Branko",
year = "2019",
abstract = "In a rat model of the andropause we aimed to examine the influence of daidzein, soy isoflavone, on the structure and function of parathyroid glands (PTG) and the expression levels of some of the crucial regulators of Ca2+ and Pi homeostasis in the kidney, and to compare these effects with the effects of estradiol, serving as a positive control. Middle-aged (16-month-old) male Wistar rats were divided into the following groups: sham-operated (SO), orchidectomized (Orx), orchidectomized and estradiol-treated (Orx+E; 0.625mg/kg b.w./day, s.c.) as well as orchidectomized and daidzein-treated (Orx+D; 30mg/kg b.w./day, s.c.) group. Every treated group had a corresponding control group. PTH serum concentration was decreased in Orx+E and Orx+D groups by 10% and 21% (p<0.05) respectively, in comparison with the Orx. PTG volume was decreased in Orx+E group by 16% (p<0.05), when compared to the Orx. In Orx+E group expression of NaPi 2a was lower (p<0.05), while NaPi 2a abundance in Orx+D animals was increased (p<0.05), when compared to Orx. Expression of PTH1R was increased (p<0.05) in Orx+E group, while in Orx+D animals the same parameter was decreased (p<0.05), in comparison with Orx. Klotho expression was elevated (p<0.05) in Orx+D rats, in regard to Orx. Orx+D induced reduction in Ca2+/creatinine and Pi/creatinine ratio in urine by 32% and 16% (p<0.05) respectively, in comparison with Orx. In conclusion, presented results indicate the more coherent beneficial effects of daidzein compared to estradiol, on disturbed Ca2+ and Pi homeostasis, and presumably on bone health, in the aging male rats.",
journal = "Annals of Anatomy = Anatomischer Anzeiger",
title = "Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause.",
volume = "221",
doi = "10.1016/j.aanat.2018.08.001",
pages = "27-37"
}

Živanović, J., Jarić, I., Ajdžanović, V., Mojić, M., Miler, M., Šošić-Jurjević, B., Milošević, V.,& Filipović, B.. (2019). Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause.. in Annals of Anatomy = Anatomischer Anzeiger, 221, 27-37.
https://doi.org/10.1016/j.aanat.2018.08.001

Živanović J, Jarić I, Ajdžanović V, Mojić M, Miler M, Šošić-Jurjević B, Milošević V, Filipović B. Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause.. in Annals of Anatomy = Anatomischer Anzeiger. 2019;221:27-37.
doi:10.1016/j.aanat.2018.08.001 .

Živanović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, Mojić, Marija, Miler, Marko, Šošić-Jurjević, Branka , Milošević, Verica, Filipović, Branko, "Daidzein upregulates anti-aging protein Klotho and NaPi 2a cotransporter in a rat model of the andropause." in Annals of Anatomy = Anatomischer Anzeiger, 221 (2019):27-37,
https://doi.org/10.1016/j.aanat.2018.08.001 . .

TY  - JOUR
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Manojlović-Stojanoski, Milica
AU  - Jarić, Ivana
AU  - Ristić, Nataša
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2018
UR  - http://doi.wiley.com/10.1002/ar.23809
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3033
AB  - The aim of the study was to examine the potential of the principal soy isoflavones, genistein and daidzein, or isoflavone rich soy extract to recover pituitary castration cells in orchidectomized adult male rats in comparison with the effects of estradiol. Two weeks post orchidectomy (Orx), animals received estradiol‐dipropionate, genistein, daidzein or soy extract subcutaneously for 3 weeks. Control sham‐operated (So) and Orx rats received just the vehicle. Changes in the volumes of pars distalis, of individual follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) containing cells, their volume, numerical density and number were determined by unbiased design‐based stereology. The intracellular content of βFSH and βLH was estimated by relative intensity of fluorescence (RIF). Orchidectomy increased all examined stereological parameters and RIF. Compared to Orx, estradiol increased the volume of pars distalis, but reversed RIF and all morphometric parameters of gonadotropes to the level of So rats, except their number. Treatments with purified isoflavones and soy extract decreased RIF to the control So level, expressing an estradiol‐like effect. However, the histological appearance and morphometrical features of gonadotropes did not follow this pattern. Genistein increased the volume of pars distalis, decreased the volume density of LH‐labeled cells and raised the number of gonadotropes. Daidzein decreased the cell volume of gonadotropic cells but increased their number and numerical density. Soy extract induced an increase in number and numerical density of FSH‐containing cells. Therefore, it can be concluded that soy phytoestrogens do not fully reverse the Orx‐induced changes in pituitary castration cells.
T2  - The Anatomical Record
T1  - Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study
IS  - 8
VL  - 301
DO  - 10.1002/ar.23809
SP  - 1416
EP  - 1425
ER  - 

@article{
author = "Nestorović, Nataša and Trifunović, Svetlana and Manojlović-Stojanoski, Milica and Jarić, Ivana and Ristić, Nataša and Filipović, Branko and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2018",
abstract = "The aim of the study was to examine the potential of the principal soy isoflavones, genistein and daidzein, or isoflavone rich soy extract to recover pituitary castration cells in orchidectomized adult male rats in comparison with the effects of estradiol. Two weeks post orchidectomy (Orx), animals received estradiol‐dipropionate, genistein, daidzein or soy extract subcutaneously for 3 weeks. Control sham‐operated (So) and Orx rats received just the vehicle. Changes in the volumes of pars distalis, of individual follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) containing cells, their volume, numerical density and number were determined by unbiased design‐based stereology. The intracellular content of βFSH and βLH was estimated by relative intensity of fluorescence (RIF). Orchidectomy increased all examined stereological parameters and RIF. Compared to Orx, estradiol increased the volume of pars distalis, but reversed RIF and all morphometric parameters of gonadotropes to the level of So rats, except their number. Treatments with purified isoflavones and soy extract decreased RIF to the control So level, expressing an estradiol‐like effect. However, the histological appearance and morphometrical features of gonadotropes did not follow this pattern. Genistein increased the volume of pars distalis, decreased the volume density of LH‐labeled cells and raised the number of gonadotropes. Daidzein decreased the cell volume of gonadotropic cells but increased their number and numerical density. Soy extract induced an increase in number and numerical density of FSH‐containing cells. Therefore, it can be concluded that soy phytoestrogens do not fully reverse the Orx‐induced changes in pituitary castration cells.",
journal = "The Anatomical Record",
title = "Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study",
number = "8",
volume = "301",
doi = "10.1002/ar.23809",
pages = "1416-1425"
}

Nestorović, N., Trifunović, S., Manojlović-Stojanoski, M., Jarić, I., Ristić, N., Filipović, B., Šošić-Jurjević, B.,& Milošević, V.. (2018). Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study. in The Anatomical Record, 301(8), 1416-1425.
https://doi.org/10.1002/ar.23809

Nestorović N, Trifunović S, Manojlović-Stojanoski M, Jarić I, Ristić N, Filipović B, Šošić-Jurjević B, Milošević V. Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study. in The Anatomical Record. 2018;301(8):1416-1425.
doi:10.1002/ar.23809 .

Nestorović, Nataša, Trifunović, Svetlana, Manojlović-Stojanoski, Milica, Jarić, Ivana, Ristić, Nataša, Filipović, Branko, Šošić-Jurjević, Branka , Milošević, Verica, "Soy Phytoestrogens Do Not Fully Reverse Changes in Rat Pituitary Castration Cells: Unbiased Stereological Study" in The Anatomical Record, 301, no. 8 (2018):1416-1425,
https://doi.org/10.1002/ar.23809 . .

TY  - JOUR
AU  - Ušćebrka, Gordana
AU  - Žikić, Dragan
AU  - Stojanović, Slobodan
AU  - Miler, Marko
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6038
AB  - One of the goals ofrnodern poultry production is improving the quality and quantity of
broiler meat. Recent research is directed to find the relation between incubation factors and
muscle development, which can affect on meat characteristics. The aim of this study was
to examine the influence of increased incubation temperature on development of breast
muscles of broilers in days after hatching. Eight hundred broiler eggs (Ross 308) were
divided in four equal groups (200 per each group) and placed in four incubators. Group
A represents control group with incubation temperature 378°C. In groups B, C, D during
the 16‘“, 17‘“ and 18th day of incubation temperature was increased to 38.5°C, 395°C and
41°C reSpectively, for 3 hours each day. On the 7‘1‘, as well as on the 14‘“ day after hatching,
10 chickens from each group were sacrificed. Samples of breast muscle (M pectoralis
superficialis) were taken for histological examination of diameter of muscle cells, volume
density of connective tissue in the muscle and nucleo-cytoplasmic ratio of muscle cells.
Measurements showed that diameter of breast muscle cells on 7L11 day after hatching in
treated groups was decreased in average by 6.36pm compared to control group, while on
 14m day after hatching in group in which incubation temperature was increased to 41°C,
it was decreased in average by 5.84pm compared to other groups. In all groups, on 14‘h
day after hatching, diameter of breast muscle cells was increased in average by 6.71pm,
while nucleo—cytoplasmic ratio of breast muscle cells was decreased in average by 0.035,
compared to 7”h day after hatching. Lower diameter of breast muscle cells in treated groups
on 7‘“ day after hatching can be explained by higher proliferation of myoblasts due to
applied treatments. During the proliferation, growth of cells is reduced, which is reflected
on lower diameter of muscle cells on 7"1 day after hatching. It can be assumed that increased
temperature during incubation affect on increased growth of muscle cells in postnatal
period of development, which is in agreement with approximately equal diameters on 14*
day after hatching between control group and groups in which incubation temperature was
increased to 385°C and 395°C. It seems that increased temperature on 41"C had negative
effect on growth and development of muscle cells, which results in decreased diameter of
breast muscle cells on 14‘h day afier hatching in group which incubation temperature was
increased to 41°C.
PB  - Skopje: Faculty of Veterinary Medicine
T2  - Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
T1  - Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching
SP  - 146
EP  - 146
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6038
ER  - 

@article{
author = "Ušćebrka, Gordana and Žikić, Dragan and Stojanović, Slobodan and Miler, Marko and Jarić, Ivana and Ajdžanović, Vladimir and Milošević, Verica",
year = "2018",
abstract = "One of the goals ofrnodern poultry production is improving the quality and quantity of
broiler meat. Recent research is directed to find the relation between incubation factors and
muscle development, which can affect on meat characteristics. The aim of this study was
to examine the influence of increased incubation temperature on development of breast
muscles of broilers in days after hatching. Eight hundred broiler eggs (Ross 308) were
divided in four equal groups (200 per each group) and placed in four incubators. Group
A represents control group with incubation temperature 378°C. In groups B, C, D during
the 16‘“, 17‘“ and 18th day of incubation temperature was increased to 38.5°C, 395°C and
41°C reSpectively, for 3 hours each day. On the 7‘1‘, as well as on the 14‘“ day after hatching,
10 chickens from each group were sacrificed. Samples of breast muscle (M pectoralis
superficialis) were taken for histological examination of diameter of muscle cells, volume
density of connective tissue in the muscle and nucleo-cytoplasmic ratio of muscle cells.
Measurements showed that diameter of breast muscle cells on 7L11 day after hatching in
treated groups was decreased in average by 6.36pm compared to control group, while on
 14m day after hatching in group in which incubation temperature was increased to 41°C,
it was decreased in average by 5.84pm compared to other groups. In all groups, on 14‘h
day after hatching, diameter of breast muscle cells was increased in average by 6.71pm,
while nucleo—cytoplasmic ratio of breast muscle cells was decreased in average by 0.035,
compared to 7”h day after hatching. Lower diameter of breast muscle cells in treated groups
on 7‘“ day after hatching can be explained by higher proliferation of myoblasts due to
applied treatments. During the proliferation, growth of cells is reduced, which is reflected
on lower diameter of muscle cells on 7"1 day after hatching. It can be assumed that increased
temperature during incubation affect on increased growth of muscle cells in postnatal
period of development, which is in agreement with approximately equal diameters on 14*
day after hatching between control group and groups in which incubation temperature was
increased to 385°C and 395°C. It seems that increased temperature on 41"C had negative
effect on growth and development of muscle cells, which results in decreased diameter of
breast muscle cells on 14‘h day afier hatching in group which incubation temperature was
increased to 41°C.",
publisher = "Skopje: Faculty of Veterinary Medicine",
journal = "Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia",
title = "Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching",
pages = "146-146",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6038"
}

Ušćebrka, G., Žikić, D., Stojanović, S., Miler, M., Jarić, I., Ajdžanović, V.,& Milošević, V.. (2018). Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
Skopje: Faculty of Veterinary Medicine., 146-146.
https://hdl.handle.net/21.15107/rcub_ibiss_6038

Ušćebrka G, Žikić D, Stojanović S, Miler M, Jarić I, Ajdžanović V, Milošević V. Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia. 2018;:146-146.
https://hdl.handle.net/21.15107/rcub_ibiss_6038 .

Ušćebrka, Gordana, Žikić, Dragan, Stojanović, Slobodan, Miler, Marko, Jarić, Ivana, Ajdžanović, Vladimir, Milošević, Verica, "Influence of Increased Incubation Temperature on Breast Muscle Development of Broilers After Hatching" in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia (2018):146-146,
https://hdl.handle.net/21.15107/rcub_ibiss_6038 .

TY  - CONF
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Živanović, Jasmina
AU  - Pendoski, Laze
AU  - Žikić, Dragan
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6037
AB  - Diosgenin is bioactive, steroidal saponin from Dioscorea viliosa (Wild Yam) and appears to be novel promising substance for the treatment of menopausal symptoms. In addition, diosgenin may manipulate the endogenous steroid hormone levels and may affect the numerous oxidative stress pathways/molecules. Since decline in the steroid hormone levels as well as increase in oxidative stress in the ovaries play an integral part of the menopause, this study aimed to investigate the effects of diosgenin on production of estradiol and progesterone, as well as to investigated the effects of diosgenin on the expression of genes encoding the antioxidant enzymes (Cu, Zn-superoxide dismutase (CuZnSOD), Mn-superoxide dismutase (MnSOD), glutathione peroxidase (GPX), and catalase (CAD) in ovary of acyclic-middle aged female rats. Middle-aged (l3-months-old) acyclic female Wistar rats in constant diestrus stage, which represent tractable model to human menopause, were divided into control and diosgenin treated groups.Diosgenin (100 mgfl<g b.w.lday) was orally applied for 4 weeks, while C group received the vehicle alone. In our experimental model, diosgenin administration to acyclic middle-aged rats significantly increased estradiol and progesterone serum concentrations when compared to the corresponding control. Gene expression analysis revealed that diosgenin significantly down-regulate all examined enzymes involved in antioxidative defense. The down-regulation were especially pronounced in CuZnSOD and CAT, indicating that the diosgenin may decrease the pool of reactive oxygen species in the ovary directly due and may act as free radical scavengers. Therefore, our results indicate beneficial potential of diosgenin in the middle-aged rat ovary.
PB  - Skopje: Faculty of Veterinary Medicine
C3  - Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia
T1  - Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats
SP  - 147
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6037
ER  - 

@conference{
author = "Jarić, Ivana and Miler, Marko and Ajdžanović, Vladimir and Živanović, Jasmina and Pendoski, Laze and Žikić, Dragan and Ušćebrka, Gordana and Milošević, Verica",
year = "2018",
abstract = "Diosgenin is bioactive, steroidal saponin from Dioscorea viliosa (Wild Yam) and appears to be novel promising substance for the treatment of menopausal symptoms. In addition, diosgenin may manipulate the endogenous steroid hormone levels and may affect the numerous oxidative stress pathways/molecules. Since decline in the steroid hormone levels as well as increase in oxidative stress in the ovaries play an integral part of the menopause, this study aimed to investigate the effects of diosgenin on production of estradiol and progesterone, as well as to investigated the effects of diosgenin on the expression of genes encoding the antioxidant enzymes (Cu, Zn-superoxide dismutase (CuZnSOD), Mn-superoxide dismutase (MnSOD), glutathione peroxidase (GPX), and catalase (CAD) in ovary of acyclic-middle aged female rats. Middle-aged (l3-months-old) acyclic female Wistar rats in constant diestrus stage, which represent tractable model to human menopause, were divided into control and diosgenin treated groups.Diosgenin (100 mgfl<g b.w.lday) was orally applied for 4 weeks, while C group received the vehicle alone. In our experimental model, diosgenin administration to acyclic middle-aged rats significantly increased estradiol and progesterone serum concentrations when compared to the corresponding control. Gene expression analysis revealed that diosgenin significantly down-regulate all examined enzymes involved in antioxidative defense. The down-regulation were especially pronounced in CuZnSOD and CAT, indicating that the diosgenin may decrease the pool of reactive oxygen species in the ovary directly due and may act as free radical scavengers. Therefore, our results indicate beneficial potential of diosgenin in the middle-aged rat ovary.",
publisher = "Skopje: Faculty of Veterinary Medicine",
journal = "Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia",
title = "Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats",
pages = "147-147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6037"
}

Jarić, I., Miler, M., Ajdžanović, V., Živanović, J., Pendoski, L., Žikić, D., Ušćebrka, G.,& Milošević, V.. (2018). Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats. in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia
Skopje: Faculty of Veterinary Medicine., 147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6037

Jarić I, Miler M, Ajdžanović V, Živanović J, Pendoski L, Žikić D, Ušćebrka G, Milošević V. Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats. in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia. 2018;:147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6037 .

Jarić, Ivana, Miler, Marko, Ajdžanović, Vladimir, Živanović, Jasmina, Pendoski, Laze, Žikić, Dragan, Ušćebrka, Gordana, Milošević, Verica, "Diosgenin Affects Steroid Hormone Production and Gene Expression of Antioxidative Enzymes in the Ovary of Middle-aged Rats" in Book of abstracts: join meeting: 5th International Vet-Istanbul group congress and 8th International scientific meeting days of veterinary medicine; 2018, Sep 23-27; Ohrid, Macedonia (2018):147-147,
https://hdl.handle.net/21.15107/rcub_ibiss_6037 .

TY  - CONF
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Šošić-Jurjević, Branka
AU  - Marković, Zorica
AU  - Potrebić, Milica
AU  - Milošević, Verica
PY  - 2018
UR  - http://physiology.org.rs/4thCPS.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6024
AB  - Advanced age is associated with development of dyslipidemia, together with ectopic triglycerides (Tg) accumulation in the liver. Consumption of citrus flavanones, naringenin (NAR) and hesperetin (HES) positively correlates with improvement of dyslipidemia in rats. However, information about this effects of citrus flavanones in old-aged rats still lack. Therefor we analyzed changes in gene expressions of liver X receptor (Lxr), thyroid hormone responsive spot14 protein (Thrsp), fatty acid synthase (Fas) and fatty acid translocase (Fat) in the liver by qPCR and serum Tg concentration biochemically. NAR or HES, suspended in vehicle (sunflower oil) were administrated orally (15 mg/kg) to male 24-month-old Wistar rats (n=5) during 4 weeks. Control groups received vehicle (CON) or stayed physiologically intact (ICON). In comparison to ICON animals, only Fat gene increased after sunflower oil administration. NAR and HES treatment decreased mRNA expression of Thrsp, Lxr and Fat genes in comparison to CON. Only NAR decreased Fas gene expression as well, while Tg concentration remained unchanged after both. All changes were statistically significant (p<0.05). In conclusion, nutritional doses of NAR or HES affect relative mRNA concentration of genes involved in control of Tg metabolism. Serum Tg did not change, but gene expression of Thrsp, Lxr and Fat was lower after both, while Fas was decreased only after NAR. This is of great importance because treatment with NAR or HES could protect liver from fatty acids and Tg de novo biosynthesis and ectopic lipid accumulation, thus preventing liver steatosis and dyslipidemia accompanied with advanced age.
PB  - Belgrade: Serbian Physiological Society
C3  - Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
T1  - Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats
SP  - 57
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6024
ER  - 

@conference{
author = "Miler, Marko and Živanović, Jasmina and Ajdžanović, Vladimir and Jarić, Ivana and Šošić-Jurjević, Branka and Marković, Zorica and Potrebić, Milica and Milošević, Verica",
year = "2018",
abstract = "Advanced age is associated with development of dyslipidemia, together with ectopic triglycerides (Tg) accumulation in the liver. Consumption of citrus flavanones, naringenin (NAR) and hesperetin (HES) positively correlates with improvement of dyslipidemia in rats. However, information about this effects of citrus flavanones in old-aged rats still lack. Therefor we analyzed changes in gene expressions of liver X receptor (Lxr), thyroid hormone responsive spot14 protein (Thrsp), fatty acid synthase (Fas) and fatty acid translocase (Fat) in the liver by qPCR and serum Tg concentration biochemically. NAR or HES, suspended in vehicle (sunflower oil) were administrated orally (15 mg/kg) to male 24-month-old Wistar rats (n=5) during 4 weeks. Control groups received vehicle (CON) or stayed physiologically intact (ICON). In comparison to ICON animals, only Fat gene increased after sunflower oil administration. NAR and HES treatment decreased mRNA expression of Thrsp, Lxr and Fat genes in comparison to CON. Only NAR decreased Fas gene expression as well, while Tg concentration remained unchanged after both. All changes were statistically significant (p<0.05). In conclusion, nutritional doses of NAR or HES affect relative mRNA concentration of genes involved in control of Tg metabolism. Serum Tg did not change, but gene expression of Thrsp, Lxr and Fat was lower after both, while Fas was decreased only after NAR. This is of great importance because treatment with NAR or HES could protect liver from fatty acids and Tg de novo biosynthesis and ectopic lipid accumulation, thus preventing liver steatosis and dyslipidemia accompanied with advanced age.",
publisher = "Belgrade: Serbian Physiological Society",
journal = "Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia",
title = "Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats",
pages = "57",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6024"
}

Miler, M., Živanović, J., Ajdžanović, V., Jarić, I., Šošić-Jurjević, B., Marković, Z., Potrebić, M.,& Milošević, V.. (2018). Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
Belgrade: Serbian Physiological Society., 57.
https://hdl.handle.net/21.15107/rcub_ibiss_6024

Miler M, Živanović J, Ajdžanović V, Jarić I, Šošić-Jurjević B, Marković Z, Potrebić M, Milošević V. Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. 2018;:57.
https://hdl.handle.net/21.15107/rcub_ibiss_6024 .

Miler, Marko, Živanović, Jasmina, Ajdžanović, Vladimir, Jarić, Ivana, Šošić-Jurjević, Branka, Marković, Zorica, Potrebić, Milica, Milošević, Verica, "Citrus flavanones alter the expression of genes involved in triglyceride metabolism in old age Wistar rats" in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences: from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia (2018):57,
https://hdl.handle.net/21.15107/rcub_ibiss_6024 .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Popovska-Perčinić, Florina
AU  - Stojanović, Slobodan
AU  - Potrebić, Milica
AU  - Sretenović, Jasmina
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5992
AB  - Advanced age is frequently entwined with the adrenal cortex function impairment, glucocorticoid excess and following metabolic syndrome.
Also, age-dependent
disturbancies in aldosterone secretion and Na*/K homeostasis at least partly underlie the hypertension issues. Classical therapeutic approach in the treatment of some menopausal symptoms implies administration of estradiol or calcium, or their combination. Herein, we aimed to investigate the effects of chronic treatment with estradiol and calcium on some adrenal gland parameters in the ageing female rat model. Middle-aged (14-month-old) female Wistar rats were treated with estradiol dipropionate (EDP; 0.625 mg/kg b.m.) and calcium glucoheptonate (Ca; 11.4 mg/kg b.m.) daily for two weeks, while control ruts received vehicle alone by the same schedule. Combined treatment caused the decrease (p<0.05) of body mass by 21.5%, while the absolute and relative adrenal gland weights were increased (p<0.05) by16.2% and 43.8% respectively, compared to the adequate control values. The absolute adrenal gland volume, after treatment with EDP and Ca in the combination, was also increased (12.1%; p<0.05), in comparison with control group.
In parallel with that, circulating aldosterone was elevated (p<0.05) by 50.0%, compared to the control levels. However, combined treatment with EDP and Ca insignificantly (P>0.05) affected Na* and K' blood concentrations. It can be concluded that simultaneous estradiol ants calcium administration to middle-aged female rats enlarges the adrenal glands and have stimulatory influence on the mineralocorticoid output, which may represent unwanted effect from the perspective of blood pressure regulation.
PB  - Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
C3  - Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
T1  - Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats
SP  - 149
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5992
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Jarić, Ivana and Popovska-Perčinić, Florina and Stojanović, Slobodan and Potrebić, Milica and Sretenović, Jasmina and Jakovljević, Vladimir and Milošević, Verica",
year = "2018",
abstract = "Advanced age is frequently entwined with the adrenal cortex function impairment, glucocorticoid excess and following metabolic syndrome.
Also, age-dependent
disturbancies in aldosterone secretion and Na*/K homeostasis at least partly underlie the hypertension issues. Classical therapeutic approach in the treatment of some menopausal symptoms implies administration of estradiol or calcium, or their combination. Herein, we aimed to investigate the effects of chronic treatment with estradiol and calcium on some adrenal gland parameters in the ageing female rat model. Middle-aged (14-month-old) female Wistar rats were treated with estradiol dipropionate (EDP; 0.625 mg/kg b.m.) and calcium glucoheptonate (Ca; 11.4 mg/kg b.m.) daily for two weeks, while control ruts received vehicle alone by the same schedule. Combined treatment caused the decrease (p<0.05) of body mass by 21.5%, while the absolute and relative adrenal gland weights were increased (p<0.05) by16.2% and 43.8% respectively, compared to the adequate control values. The absolute adrenal gland volume, after treatment with EDP and Ca in the combination, was also increased (12.1%; p<0.05), in comparison with control group.
In parallel with that, circulating aldosterone was elevated (p<0.05) by 50.0%, compared to the control levels. However, combined treatment with EDP and Ca insignificantly (P>0.05) affected Na* and K' blood concentrations. It can be concluded that simultaneous estradiol ants calcium administration to middle-aged female rats enlarges the adrenal glands and have stimulatory influence on the mineralocorticoid output, which may represent unwanted effect from the perspective of blood pressure regulation.",
publisher = "Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine",
journal = "Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia",
title = "Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats",
pages = "149",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5992"
}

Ajdžanović, V., Jarić, I., Popovska-Perčinić, F., Stojanović, S., Potrebić, M., Sretenović, J., Jakovljević, V.,& Milošević, V.. (2018). Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia
Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine., 149.
https://hdl.handle.net/21.15107/rcub_ibiss_5992

Ajdžanović V, Jarić I, Popovska-Perčinić F, Stojanović S, Potrebić M, Sretenović J, Jakovljević V, Milošević V. Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats. in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia. 2018;:149.
https://hdl.handle.net/21.15107/rcub_ibiss_5992 .

Ajdžanović, Vladimir, Jarić, Ivana, Popovska-Perčinić, Florina, Stojanović, Slobodan, Potrebić, Milica, Sretenović, Jasmina, Jakovljević, Vladimir, Milošević, Verica, "Combined treatment with estradiol and calcium enlarges the adrenals and elevates circulating aldosterone in middle-aged female rats" in Book of Abstracts: 5th International Vet-Istanbul Group Congress and 8th International Scientific Meeting: Days of Veterinary Medicine 2018; 2018 Sep 23-27; Ohrid; North Macedonia (2018):149,
https://hdl.handle.net/21.15107/rcub_ibiss_5992 .

TY  - CONF
AU  - Popovska-Perčinić, Florina
AU  - Milošević, Verica
AU  - Pendovski, Lazo
AU  - Potrebić, Milica
AU  - Ušćebrka, Gordana
AU  - Sretenović, Jasmina
AU  - Jarić, Ivana
AU  - Ajdžanović, Vladimir
PY  - 2018
UR  - http://www.enea2018.com/program.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5925
PB  - European NeuroEndocrine Association
C3  - 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
T1  - The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study
SP  - 8
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5925
ER  - 

@conference{
author = "Popovska-Perčinić, Florina and Milošević, Verica and Pendovski, Lazo and Potrebić, Milica and Ušćebrka, Gordana and Sretenović, Jasmina and Jarić, Ivana and Ajdžanović, Vladimir",
year = "2018",
publisher = "European NeuroEndocrine Association",
journal = "18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland",
title = "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study",
pages = "8",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5925"
}

Popovska-Perčinić, F., Milošević, V., Pendovski, L., Potrebić, M., Ušćebrka, G., Sretenović, J., Jarić, I.,& Ajdžanović, V.. (2018). The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland
European NeuroEndocrine Association., 8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925

Popovska-Perčinić F, Milošević V, Pendovski L, Potrebić M, Ušćebrka G, Sretenović J, Jarić I, Ajdžanović V. The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study. in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland. 2018;:8.
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .

Popovska-Perčinić, Florina, Milošević, Verica, Pendovski, Lazo, Potrebić, Milica, Ušćebrka, Gordana, Sretenović, Jasmina, Jarić, Ivana, Ajdžanović, Vladimir, "The effect of long-term exposure to moderately high ambient temperature on rat pituitary corticotropes: immunohistomorphometric and hormonal study" in 18th Congress of the European NeuroEndocrine Association; 2018 Oct 17-20; Wrocław, Poland (2018):8,
https://hdl.handle.net/21.15107/rcub_ibiss_5925 .

TY  - CONF
AU  - Živanović, Jasmina
AU  - Kouroussis, Emilia
AU  - Miler, Marko
AU  - Schott-Roux, Sonia
AU  - Jarić, Ivana
AU  - Jakovljević, Vladimir
AU  - Milošević, Verica
AU  - Filipović R., Miloš
PY  - 2018
UR  - http://physiology.org.rs/4thCPS.php
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5926
AB  - An oxidative-posttranslational modification of thiols called protein persulfidation
(RSSH) represents the underlying mechanism of biological actions ascribed to new gasotransmitter hydrogen sulfide (H2S). We developed a new dimedone based method for detection of protein persulfidation and its selectivity was tested and confirmed in various experimental models (D. melanogaster, C. elegans, M. musculus. human cell lines and samples). Obtained results demonstrated that persulfidation represents an evolutionary conserved modification that correlates to the H2S production capacity and is accompanied with numerous physiological implications. One of such general observations is that persulfidation represents protective mechanism for the reduction of sulfenic acids under conditions of oxidative stress. When C. elegans mutants for CSE and MST were exposed to different oxidative stress inducers they showed enhanced sensitivity, while pretreatment with H2S donors rescued them. Additionally, we observed a strong correlation between protein persulfidation levels and development and aging in different experimental models. Fluctuations in the level of persultides were observed during development of C. elegans with prominent increase in L3 larval stage. Our data showed that after strong peak in L3, persulfide levels decreases during life in C. elegans. Decline of persulfide levels in the adult worms of different age was also observed. Similar effect was discovered in the brains of rats of different age. Immunobistological analysis of brain sections for H2S producing enzymes revealed the decrease in intensity of immunopositivity during aging process. Our results strongly propose protein persullidation represents a rescue loop from cysteine hyper-oxidation and subsequent loss of protein function. This general mechanism represents a promising tool in overcoming the consequences of increased oxidative stress during aging process.
PB  - Belgrade: Serbian Physiological Society
C3  - Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
T1  - Anti-aging properties of protein persulfidation
SP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5926
ER  - 

@conference{
author = "Živanović, Jasmina and Kouroussis, Emilia and Miler, Marko and Schott-Roux, Sonia and Jarić, Ivana and Jakovljević, Vladimir and Milošević, Verica and Filipović R., Miloš",
year = "2018",
abstract = "An oxidative-posttranslational modification of thiols called protein persulfidation
(RSSH) represents the underlying mechanism of biological actions ascribed to new gasotransmitter hydrogen sulfide (H2S). We developed a new dimedone based method for detection of protein persulfidation and its selectivity was tested and confirmed in various experimental models (D. melanogaster, C. elegans, M. musculus. human cell lines and samples). Obtained results demonstrated that persulfidation represents an evolutionary conserved modification that correlates to the H2S production capacity and is accompanied with numerous physiological implications. One of such general observations is that persulfidation represents protective mechanism for the reduction of sulfenic acids under conditions of oxidative stress. When C. elegans mutants for CSE and MST were exposed to different oxidative stress inducers they showed enhanced sensitivity, while pretreatment with H2S donors rescued them. Additionally, we observed a strong correlation between protein persulfidation levels and development and aging in different experimental models. Fluctuations in the level of persultides were observed during development of C. elegans with prominent increase in L3 larval stage. Our data showed that after strong peak in L3, persulfide levels decreases during life in C. elegans. Decline of persulfide levels in the adult worms of different age was also observed. Similar effect was discovered in the brains of rats of different age. Immunobistological analysis of brain sections for H2S producing enzymes revealed the decrease in intensity of immunopositivity during aging process. Our results strongly propose protein persullidation represents a rescue loop from cysteine hyper-oxidation and subsequent loss of protein function. This general mechanism represents a promising tool in overcoming the consequences of increased oxidative stress during aging process.",
publisher = "Belgrade: Serbian Physiological Society",
journal = "Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia",
title = "Anti-aging properties of protein persulfidation",
pages = "143",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5926"
}

Živanović, J., Kouroussis, E., Miler, M., Schott-Roux, S., Jarić, I., Jakovljević, V., Milošević, V.,& Filipović R., M.. (2018). Anti-aging properties of protein persulfidation. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia
Belgrade: Serbian Physiological Society., 143.
https://hdl.handle.net/21.15107/rcub_ibiss_5926

Živanović J, Kouroussis E, Miler M, Schott-Roux S, Jarić I, Jakovljević V, Milošević V, Filipović R. M. Anti-aging properties of protein persulfidation. in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia. 2018;:143.
https://hdl.handle.net/21.15107/rcub_ibiss_5926 .

Živanović, Jasmina, Kouroussis, Emilia, Miler, Marko, Schott-Roux, Sonia, Jarić, Ivana, Jakovljević, Vladimir, Milošević, Verica, Filipović R., Miloš, "Anti-aging properties of protein persulfidation" in Abstract book: 4th Congress of Physiological Sciences of Serbia with International Participation: Current Trends in Physiological Sciences : from cell signals to the biology of aging; 2018 Sep 19-23; Niš, Serbia (2018):143,
https://hdl.handle.net/21.15107/rcub_ibiss_5926 .

TY  - JOUR
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Blagojević, Duško
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Nestorović, Nataša
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0041008X1730474X
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29217487
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2942
AB  - This study aimed to investigate the effects of soy isoflavones, genistein (GEN) and daidzein, (DAI) on the uterine function in ovary-intact middle-aged rats. GEN and DAI (35mg/kg) were subcutaneously administrated to acyclic (12-month-old) Wistar females, daily, for 4weeks. Control group received either vehicle (olive oil and ethanol, 9:1) or remained intact. We found that GEN and DAI differently affect uterine morphophysiology. GEN significantly increased the uterine wet weight which was associated with hyperplastic changes, revealed by stereological and histomorphometrical analyses. Also, PCNA immunoexpression was increased, whereas expression of apoptotic marker (caspase-3) was decreased. Protein and gene expressions of ERα were down-regulated, while PR and ERβ were up-regulated after GEN application. Also, GEN caused an increase of LAC and VEGF mRNA expression, together with an up-regulation of Akt activity. In contrast, DAI did not change the uterine wet weight and stereological features of the main uterine compartments as well as LAC and VEGF gene expression. Absence of hyperplastic changes were illustrated by an increase in caspase-3 immunoexpression, associated with reduced PCNA expression. DAI up-regulated only the expression of ERβ, while the expression levels of ERα and PR remain unaffected. Also, DAI inhibited the activation of Akt due to down-regulation of phosphorylated and total form of Akt protein expression. Compared to GEN, DAI did not promote events associated with the endometrial cell proliferation in the conducted study, figuring as the compound with a potential safety profile, which justifies further investigation.
T2  - Toxicology and Applied Pharmacology
T2  - Toxicology and Applied Pharmacology
T1  - Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.
VL  - 339
DO  - 10.1016/j.taap.2017.12.001
SP  - 73
EP  - 84
ER  - 

@article{
author = "Jarić, Ivana and Živanović, Jasmina and Miler, Marko and Ajdžanović, Vladimir and Blagojević, Duško and Ristić, Nataša and Milošević, Verica and Nestorović, Nataša",
year = "2018",
abstract = "This study aimed to investigate the effects of soy isoflavones, genistein (GEN) and daidzein, (DAI) on the uterine function in ovary-intact middle-aged rats. GEN and DAI (35mg/kg) were subcutaneously administrated to acyclic (12-month-old) Wistar females, daily, for 4weeks. Control group received either vehicle (olive oil and ethanol, 9:1) or remained intact. We found that GEN and DAI differently affect uterine morphophysiology. GEN significantly increased the uterine wet weight which was associated with hyperplastic changes, revealed by stereological and histomorphometrical analyses. Also, PCNA immunoexpression was increased, whereas expression of apoptotic marker (caspase-3) was decreased. Protein and gene expressions of ERα were down-regulated, while PR and ERβ were up-regulated after GEN application. Also, GEN caused an increase of LAC and VEGF mRNA expression, together with an up-regulation of Akt activity. In contrast, DAI did not change the uterine wet weight and stereological features of the main uterine compartments as well as LAC and VEGF gene expression. Absence of hyperplastic changes were illustrated by an increase in caspase-3 immunoexpression, associated with reduced PCNA expression. DAI up-regulated only the expression of ERβ, while the expression levels of ERα and PR remain unaffected. Also, DAI inhibited the activation of Akt due to down-regulation of phosphorylated and total form of Akt protein expression. Compared to GEN, DAI did not promote events associated with the endometrial cell proliferation in the conducted study, figuring as the compound with a potential safety profile, which justifies further investigation.",
journal = "Toxicology and Applied Pharmacology, Toxicology and Applied Pharmacology",
title = "Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.",
volume = "339",
doi = "10.1016/j.taap.2017.12.001",
pages = "73-84"
}

Jarić, I., Živanović, J., Miler, M., Ajdžanović, V., Blagojević, D., Ristić, N., Milošević, V.,& Nestorović, N.. (2018). Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.. in Toxicology and Applied Pharmacology, 339, 73-84.
https://doi.org/10.1016/j.taap.2017.12.001

Jarić I, Živanović J, Miler M, Ajdžanović V, Blagojević D, Ristić N, Milošević V, Nestorović N. Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats.. in Toxicology and Applied Pharmacology. 2018;339:73-84.
doi:10.1016/j.taap.2017.12.001 .

Jarić, Ivana, Živanović, Jasmina, Miler, Marko, Ajdžanović, Vladimir, Blagojević, Duško, Ristić, Nataša, Milošević, Verica, Nestorović, Nataša, "Genistein and daidzein treatments differently affect uterine homeostasis in the ovary-intact middle-aged rats." in Toxicology and Applied Pharmacology, 339 (2018):73-84,
https://doi.org/10.1016/j.taap.2017.12.001 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Tanić, Nasta
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0065128116303622
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28262328
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2605
AB  - Citrus flavanones naringenin (NAR) and hesperetin (HES) are potent antioxidants that may contribute to maintenance of health at old age by improving cardiovascular and metabolic status. However, they may also affect thyroid hormone economy. Keeping in mind impaired thyroid function at older age, in this study we tested wheather NAR or HES administration potentiate this decline. NAR or HES were administrated orally (15mg/kg) to male 24-month-old Wistar rats during 4 weeks. Control groups received vehicle, sunflower oil. Qualitative and quantitative immunohistochemical and immunofluorescent expression of specific proteins and stereological analyses of thyroid tissue were performed. Thyroid stimulating hormone (TSH) and total thyroxine (T4) concentrations were measured in serum. Thyroid parenchyma of both flavanone-treated groups was characterized by lower (p<0.05) absolute and relative volume of luminal colloid, accompanied by elevated (p<0.05) relative volume of stroma in comparison with the controls. No hypertrophy or absolute thyroid volume change was detected. Intensity of immunopositive signal for thyroglobulin (Tg) and T4 bound to Tg (T4-Tg) increased (p<0.05) in the colloid of thyroid follicles after both flavanone treatments. Serum TSH increased (p<0.05) after NAR, while T4 remained unchanged after both treatments. In conclusion, NAR elevated serum TSH in old-aged males, thus being more potent than HES in altering pituitary-thyroid axis. However, changes in thyroid structure, namely moderate colloid depletion and higher Tg and T4-Tg protein expressions after both treatments, indicate preserved capacity of the gland to compensate flavanone interfering, and maintain T4 production in old-aged males.
T2  - Acta histochemica
T1  - Citrus flavanones mildly interfere with pituitary-thyroid axis in old-aged male rats.
IS  - 3
VL  - 119
DO  - 10.1016/j.acthis.2017.02.005
SP  - 292
EP  - 301
ER  - 

@article{
author = "Miler, Marko and Jarić, Ivana and Živanović, Jasmina and Ajdžanović, Vladimir and Tanić, Nasta and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2017",
abstract = "Citrus flavanones naringenin (NAR) and hesperetin (HES) are potent antioxidants that may contribute to maintenance of health at old age by improving cardiovascular and metabolic status. However, they may also affect thyroid hormone economy. Keeping in mind impaired thyroid function at older age, in this study we tested wheather NAR or HES administration potentiate this decline. NAR or HES were administrated orally (15mg/kg) to male 24-month-old Wistar rats during 4 weeks. Control groups received vehicle, sunflower oil. Qualitative and quantitative immunohistochemical and immunofluorescent expression of specific proteins and stereological analyses of thyroid tissue were performed. Thyroid stimulating hormone (TSH) and total thyroxine (T4) concentrations were measured in serum. Thyroid parenchyma of both flavanone-treated groups was characterized by lower (p<0.05) absolute and relative volume of luminal colloid, accompanied by elevated (p<0.05) relative volume of stroma in comparison with the controls. No hypertrophy or absolute thyroid volume change was detected. Intensity of immunopositive signal for thyroglobulin (Tg) and T4 bound to Tg (T4-Tg) increased (p<0.05) in the colloid of thyroid follicles after both flavanone treatments. Serum TSH increased (p<0.05) after NAR, while T4 remained unchanged after both treatments. In conclusion, NAR elevated serum TSH in old-aged males, thus being more potent than HES in altering pituitary-thyroid axis. However, changes in thyroid structure, namely moderate colloid depletion and higher Tg and T4-Tg protein expressions after both treatments, indicate preserved capacity of the gland to compensate flavanone interfering, and maintain T4 production in old-aged males.",
journal = "Acta histochemica",
title = "Citrus flavanones mildly interfere with pituitary-thyroid axis in old-aged male rats.",
number = "3",
volume = "119",
doi = "10.1016/j.acthis.2017.02.005",
pages = "292-301"
}

Miler, M., Jarić, I., Živanović, J., Ajdžanović, V., Tanić, N., Milošević, V.,& Šošić-Jurjević, B.. (2017). Citrus flavanones mildly interfere with pituitary-thyroid axis in old-aged male rats.. in Acta histochemica, 119(3), 292-301.
https://doi.org/10.1016/j.acthis.2017.02.005

Miler M, Jarić I, Živanović J, Ajdžanović V, Tanić N, Milošević V, Šošić-Jurjević B. Citrus flavanones mildly interfere with pituitary-thyroid axis in old-aged male rats.. in Acta histochemica. 2017;119(3):292-301.
doi:10.1016/j.acthis.2017.02.005 .

Miler, Marko, Jarić, Ivana, Živanović, Jasmina, Ajdžanović, Vladimir, Tanić, Nasta, Milošević, Verica, Šošić-Jurjević, Branka , "Citrus flavanones mildly interfere with pituitary-thyroid axis in old-aged male rats." in Acta histochemica, 119, no. 3 (2017):292-301,
https://doi.org/10.1016/j.acthis.2017.02.005 . .

TY  - CONF
AU  - Šošić-Jurjević, Branka
AU  - Janković, Snežana
AU  - Lutjohann, Dieter
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Milošević, Verica
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6362
AB  - Hypercholesterolemia is associated with athero-thrombotic disease, a leading cause of mortality worldwide. Advances in both dietary and pharmacological interventions contribute to prevention and treatment of modifiable risk factors.Purified soybean phytoestrogens, isoflavones genistein (GEN) and daidzein (DAI), were reported to exert moderate cholesterol-lowering effect. In this research first we studied age-related modifications in hepatic cholesterol metabolism and serum estradiol concentration of acyclic middle-aged (MA) female rats. Then we tested if purified isoflavones may prevent or reverse these changes. Serum and hepatic total cholesterol (TCH), bile acid and cholesterol precursors were determined by comparing data obtained for MA with young adult (YA) intact (IC) females. Effects of subcutaneously administered GEN or DAI (35 mg/kg per daily during four weeks) to MA rats were evaluated vs vehicle (sterile olive oil) – treated MA females. After decapitation, perfused rat livers and serum were used to determine total cholesterol (TCH), its oxidative metabolites (7α-, 27- and 24-hydroxycholesterol) and precursors (lanosterol, desmosterol, dihydro-lano sterol and lathosterol), using gas chromatography/mass spectrometry. Serum estradiol was determined by ECLIA. MA IC females were characterized by: higher (P<0.05) serum TCH, lower (P<0.05) hepatic TCH and its biosynthetic precursors, lower (P<0.05) hepatic7α-hydroxycholesterol but elevated (P<0.05) 27- and 24- hydroxycholesterol in comparison to YA IC. Both GEN and DAI decreased (P<0.05) hepatic 27-hydroxycholesterol, with no effect on any other parameter of cholesterol metabolism. We obtained age-related reduction of serum estradiol, which was reversed by both soybean phytoestrogens. In conclusion, age-related dyslipidemia was associated with reduced 7α-hydroxycholesterol, main intermediate of neutral pathway, and higher 27—hydroxycholesterol, main intermediate of alternative acidic pathway of Chol degradation to bile acid. Both GEN and DAI lowered hepatic 27- hydroxycholesterol, which might be associated with elevated serum estradiol concentrations. Further research is needed to examine this possibility.
PB  - Bioscientifica
C3  - 19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal. (Endocrine Abstracts; Vol. 49).
T1  - Soybean phytoestrogens reduce 27-hydroxycholesterol concentration in the liver of middle-aged female rats
DO  - 10.1530/endoabs.49.EP359
DO  - 1470-3947
SP  - EP359
ER  - 

@conference{
author = "Šošić-Jurjević, Branka and Janković, Snežana and Lutjohann, Dieter and Jarić, Ivana and Miler, Marko and Filipović, Branko and Ajdžanović, Vladimir and Milošević, Verica",
year = "2017",
abstract = "Hypercholesterolemia is associated with athero-thrombotic disease, a leading cause of mortality worldwide. Advances in both dietary and pharmacological interventions contribute to prevention and treatment of modifiable risk factors.Purified soybean phytoestrogens, isoflavones genistein (GEN) and daidzein (DAI), were reported to exert moderate cholesterol-lowering effect. In this research first we studied age-related modifications in hepatic cholesterol metabolism and serum estradiol concentration of acyclic middle-aged (MA) female rats. Then we tested if purified isoflavones may prevent or reverse these changes. Serum and hepatic total cholesterol (TCH), bile acid and cholesterol precursors were determined by comparing data obtained for MA with young adult (YA) intact (IC) females. Effects of subcutaneously administered GEN or DAI (35 mg/kg per daily during four weeks) to MA rats were evaluated vs vehicle (sterile olive oil) – treated MA females. After decapitation, perfused rat livers and serum were used to determine total cholesterol (TCH), its oxidative metabolites (7α-, 27- and 24-hydroxycholesterol) and precursors (lanosterol, desmosterol, dihydro-lano sterol and lathosterol), using gas chromatography/mass spectrometry. Serum estradiol was determined by ECLIA. MA IC females were characterized by: higher (P<0.05) serum TCH, lower (P<0.05) hepatic TCH and its biosynthetic precursors, lower (P<0.05) hepatic7α-hydroxycholesterol but elevated (P<0.05) 27- and 24- hydroxycholesterol in comparison to YA IC. Both GEN and DAI decreased (P<0.05) hepatic 27-hydroxycholesterol, with no effect on any other parameter of cholesterol metabolism. We obtained age-related reduction of serum estradiol, which was reversed by both soybean phytoestrogens. In conclusion, age-related dyslipidemia was associated with reduced 7α-hydroxycholesterol, main intermediate of neutral pathway, and higher 27—hydroxycholesterol, main intermediate of alternative acidic pathway of Chol degradation to bile acid. Both GEN and DAI lowered hepatic 27- hydroxycholesterol, which might be associated with elevated serum estradiol concentrations. Further research is needed to examine this possibility.",
publisher = "Bioscientifica",
journal = "19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal. (Endocrine Abstracts; Vol. 49).",
title = "Soybean phytoestrogens reduce 27-hydroxycholesterol concentration in the liver of middle-aged female rats",
doi = "10.1530/endoabs.49.EP359, 1470-3947",
pages = "EP359"
}

Šošić-Jurjević, B., Janković, S., Lutjohann, D., Jarić, I., Miler, M., Filipović, B., Ajdžanović, V.,& Milošević, V.. (2017). Soybean phytoestrogens reduce 27-hydroxycholesterol concentration in the liver of middle-aged female rats. in 19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal. (Endocrine Abstracts; Vol. 49).
Bioscientifica., EP359.
https://doi.org/10.1530/endoabs.49.EP359

Šošić-Jurjević B, Janković S, Lutjohann D, Jarić I, Miler M, Filipović B, Ajdžanović V, Milošević V. Soybean phytoestrogens reduce 27-hydroxycholesterol concentration in the liver of middle-aged female rats. in 19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal. (Endocrine Abstracts; Vol. 49).. 2017;:EP359.
doi:10.1530/endoabs.49.EP359 .

Šošić-Jurjević, Branka, Janković, Snežana, Lutjohann, Dieter, Jarić, Ivana, Miler, Marko, Filipović, Branko, Ajdžanović, Vladimir, Milošević, Verica, "Soybean phytoestrogens reduce 27-hydroxycholesterol concentration in the liver of middle-aged female rats" in 19th European Congress of Endocrinology; 2017 May 20-23; Lisbon, Portugal. (Endocrine Abstracts; Vol. 49). (2017):EP359,
https://doi.org/10.1530/endoabs.49.EP359 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Ristić, Nataša
AU  - Milenković, Dragan
AU  - Milošević, Verica
PY  - 2017
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6106
AB  - Diosgenin, a steroidal sapogenin of natural origin, has demonstrated benefits when it comes to the treatment of malignancies, cardiovascular issues and menopausal symptoms. In this study, we investigated the histological changes of the adrenal gland after diosgenin application in a rat model of the menopause. Middle-aged, acyclic female Wistar rats were divided into control (C; n = 6) and diosgenin treated (D; n = 6) groups. Diosgenin (100 mg/kg b.w./day) was orally administered for four weeks, while C group received the vehicle alone. A histological approach included design-based stereology, histochemistry and immunohistochemistry. The adrenal cortex volume decreased in D females by 15% (p < 0.05) while the volume of adrenal medulla increased (p < 0.05) by 64%, compared to the same parameters in C group. Volume density of the zona glomerulosa (expressed per absolute adrenal gland volume) in D rats increased (p < 0.05) by 22% in comparison with C animals. Diosgenin treatment decreased (p < 0.05) the volume density of the zona fasciculata (expressed per volume of adrenal cortex) by 15% when compared to C females. Absolute volume of the zona reticularis in D group decreased (p < 0.05) by 38% in comparison with the same parameter in C rats. Also, after diosgenin application, the volume density of the zona reticularis (expressed per volume of adrenal cortex) and the zona reticularis cell volume were decreased by 51% and 20% (p < 0.05) respectively, compared to C animals. Our results, reflecting a decrease in many stereological parameters of the adrenal cortex, indicate that diosgenin took over the role of corticosteroid precursors and became incorporated into steroidogenesis
PB  - Elsevier
T2  - Acta Histochemica
T1  - Diosgenin-caused changes of the adrenal gland histological parameters in a rat model of the menopause
IS  - 1
VL  - 119
DO  - 10.1016/j.acthis.2016.11.006
SP  - 48
EP  - 56
ER  - 

@article{
author = "Ajdžanović, Vladimir and Jarić, Ivana and Miler, Marko and Filipović, Branko and Šošić-Jurjević, Branka  and Ristić, Nataša and Milenković, Dragan and Milošević, Verica",
year = "2017",
abstract = "Diosgenin, a steroidal sapogenin of natural origin, has demonstrated benefits when it comes to the treatment of malignancies, cardiovascular issues and menopausal symptoms. In this study, we investigated the histological changes of the adrenal gland after diosgenin application in a rat model of the menopause. Middle-aged, acyclic female Wistar rats were divided into control (C; n = 6) and diosgenin treated (D; n = 6) groups. Diosgenin (100 mg/kg b.w./day) was orally administered for four weeks, while C group received the vehicle alone. A histological approach included design-based stereology, histochemistry and immunohistochemistry. The adrenal cortex volume decreased in D females by 15% (p < 0.05) while the volume of adrenal medulla increased (p < 0.05) by 64%, compared to the same parameters in C group. Volume density of the zona glomerulosa (expressed per absolute adrenal gland volume) in D rats increased (p < 0.05) by 22% in comparison with C animals. Diosgenin treatment decreased (p < 0.05) the volume density of the zona fasciculata (expressed per volume of adrenal cortex) by 15% when compared to C females. Absolute volume of the zona reticularis in D group decreased (p < 0.05) by 38% in comparison with the same parameter in C rats. Also, after diosgenin application, the volume density of the zona reticularis (expressed per volume of adrenal cortex) and the zona reticularis cell volume were decreased by 51% and 20% (p < 0.05) respectively, compared to C animals. Our results, reflecting a decrease in many stereological parameters of the adrenal cortex, indicate that diosgenin took over the role of corticosteroid precursors and became incorporated into steroidogenesis",
publisher = "Elsevier",
journal = "Acta Histochemica",
title = "Diosgenin-caused changes of the adrenal gland histological parameters in a rat model of the menopause",
number = "1",
volume = "119",
doi = "10.1016/j.acthis.2016.11.006",
pages = "48-56"
}

Ajdžanović, V., Jarić, I., Miler, M., Filipović, B., Šošić-Jurjević, B., Ristić, N., Milenković, D.,& Milošević, V.. (2017). Diosgenin-caused changes of the adrenal gland histological parameters in a rat model of the menopause. in Acta Histochemica
Elsevier., 119(1), 48-56.
https://doi.org/10.1016/j.acthis.2016.11.006

Ajdžanović V, Jarić I, Miler M, Filipović B, Šošić-Jurjević B, Ristić N, Milenković D, Milošević V. Diosgenin-caused changes of the adrenal gland histological parameters in a rat model of the menopause. in Acta Histochemica. 2017;119(1):48-56.
doi:10.1016/j.acthis.2016.11.006 .

Ajdžanović, Vladimir, Jarić, Ivana, Miler, Marko, Filipović, Branko, Šošić-Jurjević, Branka , Ristić, Nataša, Milenković, Dragan, Milošević, Verica, "Diosgenin-caused changes of the adrenal gland histological parameters in a rat model of the menopause" in Acta Histochemica, 119, no. 1 (2017):48-56,
https://doi.org/10.1016/j.acthis.2016.11.006 . .

TY  - CONF
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Kardum, Nevena
AU  - Milenković, Dragan
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2017
UR  - https://www.karger.com/Article/Abstract/477987
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3501
AB  - Polyphenols are class of plant secondary metabolites that exert various beneficial effects. Namely, citrus polyphenols, naringenin (NAR) and hesperetin (HES) are potent antioxidants that may contribute to maintenance of health at old age by improving redox and metabolic status. However, polyphenols also interfere with thyroid peroxidase (TPO) activity and might affect thyroid structure and hormone production. Keeping in mind impaired thyroid function at older age, in this study we tested weather NAR or HES administration could induce structural and immunohistochemical changes in male 24-monthold Wistar rats. NAR or HES were administrated orally (15 mg/kg) during 4 weeks, while control groups received vehicle, sunflower oil. Stereological analyses (absolute and relative volume of epithelium, colloid and interstitium) and qualitative immunohistochemical examination of TPO and vascular endothelial growth factor (VEGF) proteins were performed. Results showed that thyroid structure of both flavanone-treated groups was characterized by lower (p < 0.05) absolute and relative volume of luminal colloid, accompanied by elevated (p < 0.05) relative volume of interstitium in comparison with the controls. These changes were more prominent after NAR even no hypertrophy or absolute thyroid volume change was detected. TPO expression was localized in the cytoplasm and on the apical membrane of the active follicles, showing stronger immunostaining in both flavanone treated group in comparison with the controls. VEGF immunohistochemical expression was localized in the thyrocytes’s cytoplasm of thyrocytes and C cells, being stronger in flavanone-treated animals. In conclusion, both citrus flavanones induced structural alteration of thyroid tissue. Depleted colloid and stronger apical TPO immunostaining in the thyrocytes indicate higher thyroid hormone secretion and production. In line with this, interstitium volume increment, and higher VEGF expression in thyroid parenchyma might be due to a better blood supply and the expansion of microcirculation around active follicles in the thyroid of old-aged males.
PB  - Basel: S. Karger AG
C3  - 40th Annual Meeting of the European Thyroid Association Programme; 2017 Sep 9-12; Belgrade, Serbia (European Thyroid Journal; Vol. 6; No. 1)
T1  - Polyphenols from citrus fruits induce structural and immunohistochemical changes in thyroids of old-aged male Wistar rats
DO  - 10.1159/000477987
SP  - 42
ER  - 

@conference{
author = "Miler, Marko and Ajdžanović, Vladimir and Jarić, Ivana and Živanović, Jasmina and Kardum, Nevena and Milenković, Dragan and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2017",
abstract = "Polyphenols are class of plant secondary metabolites that exert various beneficial effects. Namely, citrus polyphenols, naringenin (NAR) and hesperetin (HES) are potent antioxidants that may contribute to maintenance of health at old age by improving redox and metabolic status. However, polyphenols also interfere with thyroid peroxidase (TPO) activity and might affect thyroid structure and hormone production. Keeping in mind impaired thyroid function at older age, in this study we tested weather NAR or HES administration could induce structural and immunohistochemical changes in male 24-monthold Wistar rats. NAR or HES were administrated orally (15 mg/kg) during 4 weeks, while control groups received vehicle, sunflower oil. Stereological analyses (absolute and relative volume of epithelium, colloid and interstitium) and qualitative immunohistochemical examination of TPO and vascular endothelial growth factor (VEGF) proteins were performed. Results showed that thyroid structure of both flavanone-treated groups was characterized by lower (p < 0.05) absolute and relative volume of luminal colloid, accompanied by elevated (p < 0.05) relative volume of interstitium in comparison with the controls. These changes were more prominent after NAR even no hypertrophy or absolute thyroid volume change was detected. TPO expression was localized in the cytoplasm and on the apical membrane of the active follicles, showing stronger immunostaining in both flavanone treated group in comparison with the controls. VEGF immunohistochemical expression was localized in the thyrocytes’s cytoplasm of thyrocytes and C cells, being stronger in flavanone-treated animals. In conclusion, both citrus flavanones induced structural alteration of thyroid tissue. Depleted colloid and stronger apical TPO immunostaining in the thyrocytes indicate higher thyroid hormone secretion and production. In line with this, interstitium volume increment, and higher VEGF expression in thyroid parenchyma might be due to a better blood supply and the expansion of microcirculation around active follicles in the thyroid of old-aged males.",
publisher = "Basel: S. Karger AG",
journal = "40th Annual Meeting of the European Thyroid Association Programme; 2017 Sep 9-12; Belgrade, Serbia (European Thyroid Journal; Vol. 6; No. 1)",
title = "Polyphenols from citrus fruits induce structural and immunohistochemical changes in thyroids of old-aged male Wistar rats",
doi = "10.1159/000477987",
pages = "42"
}

Miler, M., Ajdžanović, V., Jarić, I., Živanović, J., Kardum, N., Milenković, D., Milošević, V.,& Šošić-Jurjević, B.. (2017). Polyphenols from citrus fruits induce structural and immunohistochemical changes in thyroids of old-aged male Wistar rats. in 40th Annual Meeting of the European Thyroid Association Programme; 2017 Sep 9-12; Belgrade, Serbia (European Thyroid Journal; Vol. 6; No. 1)
Basel: S. Karger AG., 42.
https://doi.org/10.1159/000477987

Miler M, Ajdžanović V, Jarić I, Živanović J, Kardum N, Milenković D, Milošević V, Šošić-Jurjević B. Polyphenols from citrus fruits induce structural and immunohistochemical changes in thyroids of old-aged male Wistar rats. in 40th Annual Meeting of the European Thyroid Association Programme; 2017 Sep 9-12; Belgrade, Serbia (European Thyroid Journal; Vol. 6; No. 1). 2017;:42.
doi:10.1159/000477987 .

Miler, Marko, Ajdžanović, Vladimir, Jarić, Ivana, Živanović, Jasmina, Kardum, Nevena, Milenković, Dragan, Milošević, Verica, Šošić-Jurjević, Branka , "Polyphenols from citrus fruits induce structural and immunohistochemical changes in thyroids of old-aged male Wistar rats" in 40th Annual Meeting of the European Thyroid Association Programme; 2017 Sep 9-12; Belgrade, Serbia (European Thyroid Journal; Vol. 6; No. 1) (2017):42,
https://doi.org/10.1159/000477987 . .

TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Lütjohann, Dieter
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Vojnović-Milutinović, Danijela
AU  - Filipović, Branko
AU  - Ajdžanović, Vladimir
AU  - Renko, Kostja
AU  - Wirth, Eva Katrin
AU  - Janković, Snežana
AU  - Kӧhrle, Josef
AU  - Milošević, Verica
PY  - 2017
UR  - http://linkinghub.elsevier.com/retrieve/pii/S053155651630585X
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2722
AB  - Soy-food and its isoflavones, genistein (G) and daidzein (D), were reported to exert mild cholesterol-lowering effect, but the underlying mechanism is still unclear. In this research, first we studied age-related alterations in hepatic cholesterol metabolism of acyclic middle-aged (MA) female rats. Then we tested if purified isoflavones may prevent or reverse these changes, and whether putative changes in hepatic thyroid hormone availability may be associated with this effect. Serum and hepatic total cholesterol (TChol), bile acid and cholesterol precursors, as well as serum TSH and T4 concentrations, hepatic deiodinase (Dio) 1 enzyme activity and MCT8 protein expression were determined by comparing data obtained for MA with young adult (YA) intact (IC) females. Effects of subcutaneously administered G or D (35 mg/kg) to MA rats were evaluated versus vehicle-treated MA females. MA IC females were characterized by: higher (p < 0.05) serum TChol, lower (p < 0.05) hepatic TChol and its biosynthetic precursors, lower (p < 0.05) hepatic 7α-hydroxycholesterol but elevated (p < 0.05) 27- and 24-hydroxycholesterol in comparison to YA IC. Both isoflavone treatments decreased (p < 0.05) hepatic 27-hydroxycholesterol, G being more effective than D, without affecting any other parameter of Chol metabolism. Only G elevated hepatic Dio1 activity (p < 0.05). In conclusion, age-related hypercholesteremia was associated with lower hepatic Chol synthesis and shift from main neutral (lower 7α-hydroxycholesterol) to alternative acidic pathway (higher 27-hydroxycholesterol) of Chol degradation to bile acid. Both isoflavones lowered hepatic 27-hydroxycholesterol, which may be considered beneficial. Only G treatment increased hepatic Dio1 activity, thus indicating local increase in thyroid hormones, obviously insufficient to induce prominent cholesterol-lowering effect.
T2  - Experimental Gerontology
T1  - Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats
VL  - 92
DO  - 10.1016/j.exger.2017.03.016
SP  - 74
EP  - 81
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Lütjohann, Dieter and Jarić, Ivana and Miler, Marko and Vojnović-Milutinović, Danijela and Filipović, Branko and Ajdžanović, Vladimir and Renko, Kostja and Wirth, Eva Katrin and Janković, Snežana and Kӧhrle, Josef and Milošević, Verica",
year = "2017",
abstract = "Soy-food and its isoflavones, genistein (G) and daidzein (D), were reported to exert mild cholesterol-lowering effect, but the underlying mechanism is still unclear. In this research, first we studied age-related alterations in hepatic cholesterol metabolism of acyclic middle-aged (MA) female rats. Then we tested if purified isoflavones may prevent or reverse these changes, and whether putative changes in hepatic thyroid hormone availability may be associated with this effect. Serum and hepatic total cholesterol (TChol), bile acid and cholesterol precursors, as well as serum TSH and T4 concentrations, hepatic deiodinase (Dio) 1 enzyme activity and MCT8 protein expression were determined by comparing data obtained for MA with young adult (YA) intact (IC) females. Effects of subcutaneously administered G or D (35 mg/kg) to MA rats were evaluated versus vehicle-treated MA females. MA IC females were characterized by: higher (p < 0.05) serum TChol, lower (p < 0.05) hepatic TChol and its biosynthetic precursors, lower (p < 0.05) hepatic 7α-hydroxycholesterol but elevated (p < 0.05) 27- and 24-hydroxycholesterol in comparison to YA IC. Both isoflavone treatments decreased (p < 0.05) hepatic 27-hydroxycholesterol, G being more effective than D, without affecting any other parameter of Chol metabolism. Only G elevated hepatic Dio1 activity (p < 0.05). In conclusion, age-related hypercholesteremia was associated with lower hepatic Chol synthesis and shift from main neutral (lower 7α-hydroxycholesterol) to alternative acidic pathway (higher 27-hydroxycholesterol) of Chol degradation to bile acid. Both isoflavones lowered hepatic 27-hydroxycholesterol, which may be considered beneficial. Only G treatment increased hepatic Dio1 activity, thus indicating local increase in thyroid hormones, obviously insufficient to induce prominent cholesterol-lowering effect.",
journal = "Experimental Gerontology",
title = "Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats",
volume = "92",
doi = "10.1016/j.exger.2017.03.016",
pages = "74-81"
}

Šošić-Jurjević, B., Lütjohann, D., Jarić, I., Miler, M., Vojnović-Milutinović, D., Filipović, B., Ajdžanović, V., Renko, K., Wirth, E. K., Janković, S., Kӧhrle, J.,& Milošević, V.. (2017). Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats. in Experimental Gerontology, 92, 74-81.
https://doi.org/10.1016/j.exger.2017.03.016

Šošić-Jurjević B, Lütjohann D, Jarić I, Miler M, Vojnović-Milutinović D, Filipović B, Ajdžanović V, Renko K, Wirth EK, Janković S, Kӧhrle J, Milošević V. Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats. in Experimental Gerontology. 2017;92:74-81.
doi:10.1016/j.exger.2017.03.016 .

Šošić-Jurjević, Branka, Lütjohann, Dieter, Jarić, Ivana, Miler, Marko, Vojnović-Milutinović, Danijela, Filipović, Branko, Ajdžanović, Vladimir, Renko, Kostja, Wirth, Eva Katrin, Janković, Snežana, Kӧhrle, Josef, Milošević, Verica, "Effects of age and soybean isoflavones on hepatic cholesterol metabolism and thyroid hormone availability in acyclic female rats" in Experimental Gerontology, 92 (2017):74-81,
https://doi.org/10.1016/j.exger.2017.03.016 . .

TY  - CONF
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Manojlović-Stojanoski, Milica
AU  - Jarić, Ivana
AU  - Ristić, Nataša
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Milošević, Verica
PY  - 2017
UR  - https://mcm2017.irb.hr/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3429
AB  - Daidzein is a soybeans-derived isoflavone that has structural and functional similarity to 17 beta-estradiol. It competes with endogenous estrogens for binding to estrogen receptors with preferential binding affinity for estrogen receptor beta over alpha (1). The aim of this study was to examine the potential of daidzein to recover castration cells after long-term orchidectomy (Orx) of adult rats, by quantifying changes in their morphology and intracellular hormone content. To that end we have used design-based stereology that still remains one of the pillars of quantitative biomedical research. Estradiol-dipropionate treatment was used as a positive control. Two weeks post orchidectomy (Orx), adult male rats subcutaneously received 30 mg of daidzein or 0.625 mg of estradiol-dipropionate per kg b.w. for three weeks. Control sham-operated (So) and Orx rats were injected with the solvent. Changes in the volume of pituitary pars distalis, the volumes of individual follicle-stimulating hormone (FSH) and luteinizing hormone (LH) immunoreactive cells, their volume and numerical densities, and number per pars distalis were determined. The FSH and LH intracellular content was estimated by relative intensity of fluorescence. Orchidectomy caused hyperstimulation and hyperplasia of gonadotropes and formation of castration cells that are characterized by prominent vacuolation of cytoplasm. All examined stereological parameters and relative intensity of fluorescence were increased comparing to So controls. After estradiol treatment, gonadotropic cells were smaller in size, polyhedral in shape with homogenous cytoplasm. Volume of gonadotropic cells, their volume and numerical density, as well as intracellular hormone contents, significantly decreased comparing to Orx controls. The parameters that did not return to the level of So controls and in contrast increased, are number of gonadotropic cells and volume of pars distalis. Castration cells in the pituitaries of daidzein-treated rats appeared smaller comparing
408
to those of Orx males, but vacuolation of cytoplasm was still apparent. Significant decrease of volume of FSH- and LH-labeled cells by 15.4% and 14.4%, respectively, was observed. Additionally, intracellular FSH and LH contents decreased by 50.1% and 41.9%, respectively. Comparing to the values of Orx controls, the number of FSH- and LH-immunoreactive cells per pituitary pars distalis increased by 37.7% and 72.1%, respectively, upon daidzein treatment. The volume density of gonadotropes and the volume of pars distalis remained unchanged. The results of this study suggest that daidzein had an estradiol-like effect by reversing some of examined parameters. However, having in mind that volume and numerical densities of gonadotropic cells remained unchanged or increased, respectively, it can be concluded that dadzein does not have potential to fully recover castration cells in the model of adult Orx rats.
PB  - Ruđer Bošković Institute and Croatian Microscopy Society
C3  - 13th Multinational Congress on Microscopy: Book of Abstract; Rovinj, Croatia; 2017 Sep 24-29
T1  - Daidzein does not fully reverse changes of pituitary castration cells in rat: immunohistochemical and stereological study
SP  - 407
EP  - 408
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3429
ER  - 

@conference{
author = "Nestorović, Nataša and Trifunović, Svetlana and Manojlović-Stojanoski, Milica and Jarić, Ivana and Ristić, Nataša and Filipović, Branko and Šošić-Jurjević, Branka  and Milošević, Verica",
year = "2017",
abstract = "Daidzein is a soybeans-derived isoflavone that has structural and functional similarity to 17 beta-estradiol. It competes with endogenous estrogens for binding to estrogen receptors with preferential binding affinity for estrogen receptor beta over alpha (1). The aim of this study was to examine the potential of daidzein to recover castration cells after long-term orchidectomy (Orx) of adult rats, by quantifying changes in their morphology and intracellular hormone content. To that end we have used design-based stereology that still remains one of the pillars of quantitative biomedical research. Estradiol-dipropionate treatment was used as a positive control. Two weeks post orchidectomy (Orx), adult male rats subcutaneously received 30 mg of daidzein or 0.625 mg of estradiol-dipropionate per kg b.w. for three weeks. Control sham-operated (So) and Orx rats were injected with the solvent. Changes in the volume of pituitary pars distalis, the volumes of individual follicle-stimulating hormone (FSH) and luteinizing hormone (LH) immunoreactive cells, their volume and numerical densities, and number per pars distalis were determined. The FSH and LH intracellular content was estimated by relative intensity of fluorescence. Orchidectomy caused hyperstimulation and hyperplasia of gonadotropes and formation of castration cells that are characterized by prominent vacuolation of cytoplasm. All examined stereological parameters and relative intensity of fluorescence were increased comparing to So controls. After estradiol treatment, gonadotropic cells were smaller in size, polyhedral in shape with homogenous cytoplasm. Volume of gonadotropic cells, their volume and numerical density, as well as intracellular hormone contents, significantly decreased comparing to Orx controls. The parameters that did not return to the level of So controls and in contrast increased, are number of gonadotropic cells and volume of pars distalis. Castration cells in the pituitaries of daidzein-treated rats appeared smaller comparing
408
to those of Orx males, but vacuolation of cytoplasm was still apparent. Significant decrease of volume of FSH- and LH-labeled cells by 15.4% and 14.4%, respectively, was observed. Additionally, intracellular FSH and LH contents decreased by 50.1% and 41.9%, respectively. Comparing to the values of Orx controls, the number of FSH- and LH-immunoreactive cells per pituitary pars distalis increased by 37.7% and 72.1%, respectively, upon daidzein treatment. The volume density of gonadotropes and the volume of pars distalis remained unchanged. The results of this study suggest that daidzein had an estradiol-like effect by reversing some of examined parameters. However, having in mind that volume and numerical densities of gonadotropic cells remained unchanged or increased, respectively, it can be concluded that dadzein does not have potential to fully recover castration cells in the model of adult Orx rats.",
publisher = "Ruđer Bošković Institute and Croatian Microscopy Society",
journal = "13th Multinational Congress on Microscopy: Book of Abstract; Rovinj, Croatia; 2017 Sep 24-29",
title = "Daidzein does not fully reverse changes of pituitary castration cells in rat: immunohistochemical and stereological study",
pages = "407-408",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3429"
}

Nestorović, N., Trifunović, S., Manojlović-Stojanoski, M., Jarić, I., Ristić, N., Filipović, B., Šošić-Jurjević, B.,& Milošević, V.. (2017). Daidzein does not fully reverse changes of pituitary castration cells in rat: immunohistochemical and stereological study. in 13th Multinational Congress on Microscopy: Book of Abstract; Rovinj, Croatia; 2017 Sep 24-29
Ruđer Bošković Institute and Croatian Microscopy Society., 407-408.
https://hdl.handle.net/21.15107/rcub_ibiss_3429

Nestorović N, Trifunović S, Manojlović-Stojanoski M, Jarić I, Ristić N, Filipović B, Šošić-Jurjević B, Milošević V. Daidzein does not fully reverse changes of pituitary castration cells in rat: immunohistochemical and stereological study. in 13th Multinational Congress on Microscopy: Book of Abstract; Rovinj, Croatia; 2017 Sep 24-29. 2017;:407-408.
https://hdl.handle.net/21.15107/rcub_ibiss_3429 .

Nestorović, Nataša, Trifunović, Svetlana, Manojlović-Stojanoski, Milica, Jarić, Ivana, Ristić, Nataša, Filipović, Branko, Šošić-Jurjević, Branka , Milošević, Verica, "Daidzein does not fully reverse changes of pituitary castration cells in rat: immunohistochemical and stereological study" in 13th Multinational Congress on Microscopy: Book of Abstract; Rovinj, Croatia; 2017 Sep 24-29 (2017):407-408,
https://hdl.handle.net/21.15107/rcub_ibiss_3429 .

TY  - CONF
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Živanović, Jasmina
AU  - Manojlović-Stojanoski, Milica
AU  - Nestorović, Nataša
AU  - Trifunović, Svetlana
AU  - Jarić, Ivana
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3432
AB  - Calcitonin (CT) is a hypocalcemic hormone produced by thyroid C-cells that acts as an bone antiresorptive agent. The other endocrine cell population in thyroid, called follicular cells produce thyroid hormones (TH) which also affect bone turnover. In this study, we evaluated the effects of salmon CT administration on structure and function of both CT and TH producing thyroid cells as well as trabecular bone microarchitecture in orchidectomized (Orx) middle-aged rats. Fifteen-month-old male Wistar rats were either Orx or sham-operated (SO). One group of Orx animals were injected subcutaneously with synthetic salmon CT (Orx + CT; 100 IU/kg b.w.) every second day for 6 weeks. The rats from SO and second Orx group received the same volume of vehicle alone by the same schedule. The peroxidase–antiperoxidase method was applied for localization of CT in C-cells. CT-immunopositive thyroid C-cells, thyroid follicular epithelium, interstitium and colloid were evaluated morphometrically. An ImageJ public domain image processing program was used to measure bone histomorphometric parameters of the proximal tibial specimens. Blood serum samples were analyzed for CT, osteocalcin (OC) and thyroxine (T4), and urine samples fot calcium (Ca2+) concentration. We found a significant decrease in the Vc and Vv of thyroid C-cells after CT treatment compared to both SO and Orx. The Vv of the colloid was higher, while VV of the follicular epithelium was lower after CT treatment compared to Orx. Analysis of trabecular microarchitecture showed that salmon CT administration significantly increases of cancellous bone area (B.Ar), trabecular thickness (Tb.Th), and trabecular number (Tb.N) whereas trabecular separation (Tb.Sp) was significantly decreased. CT treatment markedly elevated serum CT, but serum OC, T4 and urinary Ca2+ concentrations were lower than in the Orx group. These findings indicate that administration of salmon CT inhibited calcitonin-producing thyroid C-cells and changes the structure of the thyroid gland indicating hypoactivity.
PB  - BioScientifica
C3  - 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49)
T1  - Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis
DO  - 10.1530/endoabs.49.EP212
SP  - EP212
ER  - 

@conference{
author = "Filipović, Branko and Šošić-Jurjević, Branka  and Živanović, Jasmina and Manojlović-Stojanoski, Milica and Nestorović, Nataša and Trifunović, Svetlana and Jarić, Ivana and Ušćebrka, Gordana and Milošević, Verica",
year = "2017",
abstract = "Calcitonin (CT) is a hypocalcemic hormone produced by thyroid C-cells that acts as an bone antiresorptive agent. The other endocrine cell population in thyroid, called follicular cells produce thyroid hormones (TH) which also affect bone turnover. In this study, we evaluated the effects of salmon CT administration on structure and function of both CT and TH producing thyroid cells as well as trabecular bone microarchitecture in orchidectomized (Orx) middle-aged rats. Fifteen-month-old male Wistar rats were either Orx or sham-operated (SO). One group of Orx animals were injected subcutaneously with synthetic salmon CT (Orx + CT; 100 IU/kg b.w.) every second day for 6 weeks. The rats from SO and second Orx group received the same volume of vehicle alone by the same schedule. The peroxidase–antiperoxidase method was applied for localization of CT in C-cells. CT-immunopositive thyroid C-cells, thyroid follicular epithelium, interstitium and colloid were evaluated morphometrically. An ImageJ public domain image processing program was used to measure bone histomorphometric parameters of the proximal tibial specimens. Blood serum samples were analyzed for CT, osteocalcin (OC) and thyroxine (T4), and urine samples fot calcium (Ca2+) concentration. We found a significant decrease in the Vc and Vv of thyroid C-cells after CT treatment compared to both SO and Orx. The Vv of the colloid was higher, while VV of the follicular epithelium was lower after CT treatment compared to Orx. Analysis of trabecular microarchitecture showed that salmon CT administration significantly increases of cancellous bone area (B.Ar), trabecular thickness (Tb.Th), and trabecular number (Tb.N) whereas trabecular separation (Tb.Sp) was significantly decreased. CT treatment markedly elevated serum CT, but serum OC, T4 and urinary Ca2+ concentrations were lower than in the Orx group. These findings indicate that administration of salmon CT inhibited calcitonin-producing thyroid C-cells and changes the structure of the thyroid gland indicating hypoactivity.",
publisher = "BioScientifica",
journal = "19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49)",
title = "Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis",
doi = "10.1530/endoabs.49.EP212",
pages = "EP212"
}

Filipović, B., Šošić-Jurjević, B., Živanović, J., Manojlović-Stojanoski, M., Nestorović, N., Trifunović, S., Jarić, I., Ušćebrka, G.,& Milošević, V.. (2017). Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis. in 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49)
BioScientifica., EP212.
https://doi.org/10.1530/endoabs.49.EP212

Filipović B, Šošić-Jurjević B, Živanović J, Manojlović-Stojanoski M, Nestorović N, Trifunović S, Jarić I, Ušćebrka G, Milošević V. Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis. in 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49). 2017;:EP212.
doi:10.1530/endoabs.49.EP212 .

Filipović, Branko, Šošić-Jurjević, Branka , Živanović, Jasmina, Manojlović-Stojanoski, Milica, Nestorović, Nataša, Trifunović, Svetlana, Jarić, Ivana, Ušćebrka, Gordana, Milošević, Verica, "Salmon calcitonin affects thyroid endocrine cells and trabecular bone in a rat model of male osteoporosis" in 19th European Congress of Endocrinology; Lisbon, Portugal; 2017 May 20-23. (Endocrine Abstracts; Vol. 49) (2017):EP212,
https://doi.org/10.1530/endoabs.49.EP212 . .

TY  - JOUR
AU  - Popovska-Perčinić, Florina
AU  - Jarić, Ivana
AU  - Pendovski, Lazo
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Milošević, Verica
AU  - Ajdžanović, Vladimir
PY  - 2017
UR  - http://www.degruyter.com/view/j/acve.2017.67.issue-4/acve-2017-0040/acve-2017-0040.xml
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2994
AB  - In areas with moderate continental climate, increased average ambient temperature during the summer represents a stressogenic factor that affects the hypothalamo-pituitaryadrenocortical axis in mammals. Therefore, we wanted to examine the effects of 4 days of constant exposure to moderately elevated ambient temperature (35 ± 1oC) on the histomorphometric and immunofl uorescent characteristics, as well as on the hormonal secretion of pituitary corticotropes (ACTH) cells in adult male rats. In comparison with the controls kept at 20 ± 2oC, a signifi cant increase (p<0.05) of the absolute and relative pituitary weight (23.1% and 36.1%, respectively) was registered after exposure to heat. The localization, as well as the shape of the ACTH cells in the heat exposed group was not signifi cantly altered, but their immunopositivity was weaker. After 4 days of heat exposure, a weaker signal confi rmed the relative fl uorescence intensity of the ACTH cells (15.3%, p<0.05). In heat exposed rats, an increase of the cellular and nuclear volumes of immunolabelled ACTH cells and decrease of their volume density (6.9%, 14.3% and 20.0%, respectively; p<0.05) was registered. Observed histomorphometric and immunofl uorescent features of the pituitary ACTH cells were in accordance with the increased (p<0.05) value of plasma adrenocorticotropic hormone (ACTH) by 23.7% compared to the control rats. It can be concluded that the 4-day exposure to moderately elevated ambient temperature intensifi es pituitary ACTH secretion in adult male rats.
T2  - Acta Veterinaria
T1  - The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study
IS  - 4
VL  - 67
DO  - 10.1515/acve-2017-0040
SP  - 495
EP  - 507
ER  - 

@article{
author = "Popovska-Perčinić, Florina and Jarić, Ivana and Pendovski, Lazo and Ristić, Nataša and Trifunović, Svetlana and Milošević, Verica and Ajdžanović, Vladimir",
year = "2017",
abstract = "In areas with moderate continental climate, increased average ambient temperature during the summer represents a stressogenic factor that affects the hypothalamo-pituitaryadrenocortical axis in mammals. Therefore, we wanted to examine the effects of 4 days of constant exposure to moderately elevated ambient temperature (35 ± 1oC) on the histomorphometric and immunofl uorescent characteristics, as well as on the hormonal secretion of pituitary corticotropes (ACTH) cells in adult male rats. In comparison with the controls kept at 20 ± 2oC, a signifi cant increase (p<0.05) of the absolute and relative pituitary weight (23.1% and 36.1%, respectively) was registered after exposure to heat. The localization, as well as the shape of the ACTH cells in the heat exposed group was not signifi cantly altered, but their immunopositivity was weaker. After 4 days of heat exposure, a weaker signal confi rmed the relative fl uorescence intensity of the ACTH cells (15.3%, p<0.05). In heat exposed rats, an increase of the cellular and nuclear volumes of immunolabelled ACTH cells and decrease of their volume density (6.9%, 14.3% and 20.0%, respectively; p<0.05) was registered. Observed histomorphometric and immunofl uorescent features of the pituitary ACTH cells were in accordance with the increased (p<0.05) value of plasma adrenocorticotropic hormone (ACTH) by 23.7% compared to the control rats. It can be concluded that the 4-day exposure to moderately elevated ambient temperature intensifi es pituitary ACTH secretion in adult male rats.",
journal = "Acta Veterinaria",
title = "The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study",
number = "4",
volume = "67",
doi = "10.1515/acve-2017-0040",
pages = "495-507"
}

Popovska-Perčinić, F., Jarić, I., Pendovski, L., Ristić, N., Trifunović, S., Milošević, V.,& Ajdžanović, V.. (2017). The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study. in Acta Veterinaria, 67(4), 495-507.
https://doi.org/10.1515/acve-2017-0040

Popovska-Perčinić F, Jarić I, Pendovski L, Ristić N, Trifunović S, Milošević V, Ajdžanović V. The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study. in Acta Veterinaria. 2017;67(4):495-507.
doi:10.1515/acve-2017-0040 .

Popovska-Perčinić, Florina, Jarić, Ivana, Pendovski, Lazo, Ristić, Nataša, Trifunović, Svetlana, Milošević, Verica, Ajdžanović, Vladimir, "The Effect of Moderate Heat on Rat Pituitary ACTH Cells: Histomorphometric, Immunofluorescent and Hormonal Study" in Acta Veterinaria, 67, no. 4 (2017):495-507,
https://doi.org/10.1515/acve-2017-0040 . .

TY  - JOUR
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Filipović, Branko
AU  - Šošić-Jurjević, Branka 
AU  - Ristić, Nataša
AU  - Stanković, Sanja D.
AU  - Milošević, Verica
PY  - 2016
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26951517
UR  - http://www.hh.um.es/Abstracts/Vol_31/31_11/31_11_1209.htm
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2469
AB  - Histological analysis of the adrenal cortex, after testosterone application in a rat model of the andropause, was the main subject of the present study. Middle-aged Wistar rats were divided into sham-operated (SO; n=8), orchidectomized (Orx; n=8) and testosterone treated orchidectomized (Orx+T; n=8) groups. Testosterone propionate (5 mg/kg b.w. /day) was administered for three weeks, while SO and Orx groups received the vehicle alone. Histological objectives were achieved using stereology, histochemistry and steroid receptor immunostaining. The concentrations of testosterone, aldosterone, corticosterone and DHEA were determined by immunoassays. Expectedly, increased (p<0.05) serum concentration of testosterone was observed in Orx+T group. The volume of ZG cells and nuclei increased in Orx+T animals by 50% and 25% (p<0.05) respectively, but the serum concentrations of aldosterone decreased (p<0.05) by 60%, all compared to the same parameters in Orx group. The immunostaining for androgen receptors (ARs) suggested their cytoplasmic localization in ZG cells of Orx+T rats. Volume of the ZF cell nuclei in Orx+T group decreased (p<0.05) by 17%, which was followed by the significant (p<0.05) fall in corticosterone production and secretion, all in comparison with Orx animals. Also, nuclear immunolocalization of ARs of high optical density was observed through the ZF of Orx+T group. In Orx+T rats volume of ZR cells and nuclei, and circulating DHEA concentration increased (p<0.05) by 68%, 22% and about 6.6 times respectively, compared to Orx animals. Besides the extra-receptor actions in adrenal cortex, testosterone supposedly affects some steroidogenesis-related gene expression, as indicated by centripetal rise in the number of nuclear ARs.
PB  - Sociedad Española de Histología e Ingeniería Tisular, University of Murcia
T2  - Histology and Histopathology
T1  - Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause
IS  - 11
VL  - 31
DO  - 10.14670/HH-11-749
SP  - 1209
EP  - 20
ER  - 

@article{
author = "Ajdžanović, Vladimir and Jarić, Ivana and Živanović, Jasmina and Filipović, Branko and Šošić-Jurjević, Branka  and Ristić, Nataša and Stanković, Sanja D. and Milošević, Verica",
year = "2016",
abstract = "Histological analysis of the adrenal cortex, after testosterone application in a rat model of the andropause, was the main subject of the present study. Middle-aged Wistar rats were divided into sham-operated (SO; n=8), orchidectomized (Orx; n=8) and testosterone treated orchidectomized (Orx+T; n=8) groups. Testosterone propionate (5 mg/kg b.w. /day) was administered for three weeks, while SO and Orx groups received the vehicle alone. Histological objectives were achieved using stereology, histochemistry and steroid receptor immunostaining. The concentrations of testosterone, aldosterone, corticosterone and DHEA were determined by immunoassays. Expectedly, increased (p<0.05) serum concentration of testosterone was observed in Orx+T group. The volume of ZG cells and nuclei increased in Orx+T animals by 50% and 25% (p<0.05) respectively, but the serum concentrations of aldosterone decreased (p<0.05) by 60%, all compared to the same parameters in Orx group. The immunostaining for androgen receptors (ARs) suggested their cytoplasmic localization in ZG cells of Orx+T rats. Volume of the ZF cell nuclei in Orx+T group decreased (p<0.05) by 17%, which was followed by the significant (p<0.05) fall in corticosterone production and secretion, all in comparison with Orx animals. Also, nuclear immunolocalization of ARs of high optical density was observed through the ZF of Orx+T group. In Orx+T rats volume of ZR cells and nuclei, and circulating DHEA concentration increased (p<0.05) by 68%, 22% and about 6.6 times respectively, compared to Orx animals. Besides the extra-receptor actions in adrenal cortex, testosterone supposedly affects some steroidogenesis-related gene expression, as indicated by centripetal rise in the number of nuclear ARs.",
publisher = "Sociedad Española de Histología e Ingeniería Tisular, University of Murcia",
journal = "Histology and Histopathology",
title = "Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause",
number = "11",
volume = "31",
doi = "10.14670/HH-11-749",
pages = "1209-20"
}

Ajdžanović, V., Jarić, I., Živanović, J., Filipović, B., Šošić-Jurjević, B., Ristić, N., Stanković, S. D.,& Milošević, V.. (2016). Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause. in Histology and Histopathology
Sociedad Española de Histología e Ingeniería Tisular, University of Murcia., 31(11), 1209-20.
https://doi.org/10.14670/HH-11-749

Ajdžanović V, Jarić I, Živanović J, Filipović B, Šošić-Jurjević B, Ristić N, Stanković SD, Milošević V. Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause. in Histology and Histopathology. 2016;31(11):1209-20.
doi:10.14670/HH-11-749 .

Ajdžanović, Vladimir, Jarić, Ivana, Živanović, Jasmina, Filipović, Branko, Šošić-Jurjević, Branka , Ristić, Nataša, Stanković, Sanja D., Milošević, Verica, "Histological parameters of the adrenal cortex after testosterone application in a rat model of the andropause" in Histology and Histopathology, 31, no. 11 (2016):1209-20,
https://doi.org/10.14670/HH-11-749 . .

TY  - JOUR
AU  - Spasojević, Dragica
AU  - Zmejkoski, Danica
AU  - Glamočlija, Jasmina
AU  - Nikolić, Miloš
AU  - Soković, Marina
AU  - Milošević, Verica
AU  - Jarić, Ivana
AU  - Stojanović, Marijana
AU  - Marinković, Emilija
AU  - Barisani-Asenbauer, Talin
AU  - Prodanović, Radivoje
AU  - Jovanović, Miloš
AU  - Radotić, Ksenija
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0924857916302679
UR  - https://www.scopus.com/record/display.uri?eid=2-s2.0-85000995852&origin=SingleRecordEmailAlert&dgcid=scalert_sc_search_email&txGid=5EA0B882AD39CF37A9F768C8B10917B5.wsnAw8kcdt7IPYLO0V48gA%3A1#
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2480
AB  - Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP−Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments.
T2  - International Journal of Antimicrobial Agents
T1  - Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment
IS  - 6
VL  - 48
DO  - 10.1016/j.ijantimicag.2016.08.014
SP  - 732
EP  - 735
ER  - 

@article{
author = "Spasojević, Dragica and Zmejkoski, Danica and Glamočlija, Jasmina and Nikolić, Miloš and Soković, Marina and Milošević, Verica and Jarić, Ivana and Stojanović, Marijana and Marinković, Emilija and Barisani-Asenbauer, Talin and Prodanović, Radivoje and Jovanović, Miloš and Radotić, Ksenija",
year = "2016",
abstract = "Nowadays bacterial resistance to known antibiotics is a serious health problem. In order to achieve more efficient treatment, lately there is an effort to find new substances, such as certain biomaterials, that are non-toxic to humans with antibiotic potential. Lignins and lignin-derived compounds have been proposed to be good candidates for use in medicine and health maintenance. In this study, the antibacterial activity of the lignin model polymer dehydrogenate polymer (DHP) in alginate hydrogel (Alg) was studied. The obtained results show that DHP−Alg has strong antimicrobial activity against several bacterial strains and biofilms and does not have a toxic effect on human epithelial cells. These results strongly suggest its application as a wound healing agent or as an adjunct substance for wound treatments.",
journal = "International Journal of Antimicrobial Agents",
title = "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment",
number = "6",
volume = "48",
doi = "10.1016/j.ijantimicag.2016.08.014",
pages = "732-735"
}

Spasojević, D., Zmejkoski, D., Glamočlija, J., Nikolić, M., Soković, M., Milošević, V., Jarić, I., Stojanović, M., Marinković, E., Barisani-Asenbauer, T., Prodanović, R., Jovanović, M.,& Radotić, K.. (2016). Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment. in International Journal of Antimicrobial Agents, 48(6), 732-735.
https://doi.org/10.1016/j.ijantimicag.2016.08.014

Spasojević D, Zmejkoski D, Glamočlija J, Nikolić M, Soković M, Milošević V, Jarić I, Stojanović M, Marinković E, Barisani-Asenbauer T, Prodanović R, Jovanović M, Radotić K. Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment. in International Journal of Antimicrobial Agents. 2016;48(6):732-735.
doi:10.1016/j.ijantimicag.2016.08.014 .

Spasojević, Dragica, Zmejkoski, Danica, Glamočlija, Jasmina, Nikolić, Miloš, Soković, Marina, Milošević, Verica, Jarić, Ivana, Stojanović, Marijana, Marinković, Emilija, Barisani-Asenbauer, Talin, Prodanović, Radivoje, Jovanović, Miloš, Radotić, Ksenija, "Lignin model compound in alginate hydrogel: a strong antimicrobial agent with high potential in wound treatment" in International Journal of Antimicrobial Agents, 48, no. 6 (2016):732-735,
https://doi.org/10.1016/j.ijantimicag.2016.08.014 . .

TY  - CONF
AU  - Ajdžanović, Vladimir
AU  - Šošić-Jurjević, Branka
AU  - Filipović, Branko
AU  - Popovska-Perčinić, Florina
AU  - Jarić, Ivana
AU  - Miler, Marko
AU  - Ušćebrka, Gordana
AU  - Milošević, Verica
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6280
AB  - Introduction: Genistein is widely used as an alternative therapeutic for various pathological conditions in ageing male and exerts an inhibitory effect on the steroidogenic enzyme families, thus affecting hormonal homeostasis. The aim of this study was to examine the effects of genistein on histological features of the adrenal cortex zona reticularis and the following dehydroepiandrosterone (DHEA) secretion in orchidectomized adult male rats, the most accessible model reflecting andropause.
Material and Methods: The experimental adult Wistar male rats were divided into three groups (n=7): sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. All animal were sacrificed by decapitation 24h after the last injection. The adrenal cortex was analyzed histologically, while serum concentration of DHEA was determined by immunoassay.
Results: Histological analysis revealed that there were no significant changes in adrenal cortex zona reticularis after orchidectomy and orchidectomy followed with genistein treatment. Circulating DHEA concentration was significantly (p<0.005) increased by 97.2% in Orx animals compared to the SO group. In Orx+G group serum DHEA was significantly (p<0.005) decreased by 41.5% in comparison with Orx rats. Serum concentration of DHEA upon genistein application to orchidectomized animals was insignificantly (p>0.05) changed by 15.3% when compared to the SO group.
Conclusion: These data, observed in orchidectomized adult rats, indicate that genistein has an inhibitory effect on the adrenal cortex zona reticularis secretory function.
PB  - Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine
C3  - Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia
T1  - Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein
SP  - 130
EP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6280
ER  - 

@conference{
author = "Ajdžanović, Vladimir and Šošić-Jurjević, Branka and Filipović, Branko and Popovska-Perčinić, Florina and Jarić, Ivana and Miler, Marko and Ušćebrka, Gordana and Milošević, Verica",
year = "2016",
abstract = "Introduction: Genistein is widely used as an alternative therapeutic for various pathological conditions in ageing male and exerts an inhibitory effect on the steroidogenic enzyme families, thus affecting hormonal homeostasis. The aim of this study was to examine the effects of genistein on histological features of the adrenal cortex zona reticularis and the following dehydroepiandrosterone (DHEA) secretion in orchidectomized adult male rats, the most accessible model reflecting andropause.
Material and Methods: The experimental adult Wistar male rats were divided into three groups (n=7): sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30 mg/kg b.m./day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. All animal were sacrificed by decapitation 24h after the last injection. The adrenal cortex was analyzed histologically, while serum concentration of DHEA was determined by immunoassay.
Results: Histological analysis revealed that there were no significant changes in adrenal cortex zona reticularis after orchidectomy and orchidectomy followed with genistein treatment. Circulating DHEA concentration was significantly (p<0.005) increased by 97.2% in Orx animals compared to the SO group. In Orx+G group serum DHEA was significantly (p<0.005) decreased by 41.5% in comparison with Orx rats. Serum concentration of DHEA upon genistein application to orchidectomized animals was insignificantly (p>0.05) changed by 15.3% when compared to the SO group.
Conclusion: These data, observed in orchidectomized adult rats, indicate that genistein has an inhibitory effect on the adrenal cortex zona reticularis secretory function.",
publisher = "Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine",
journal = "Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia",
title = "Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein",
pages = "130-131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6280"
}

Ajdžanović, V., Šošić-Jurjević, B., Filipović, B., Popovska-Perčinić, F., Jarić, I., Miler, M., Ušćebrka, G.,& Milošević, V.. (2016). Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein. in Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia
Skopje: Ss. Cyril and Methodius University in Skopje, Faculty of Veterinary Medicine., 130-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6280

Ajdžanović V, Šošić-Jurjević B, Filipović B, Popovska-Perčinić F, Jarić I, Miler M, Ušćebrka G, Milošević V. Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein. in Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia. 2016;:130-131.
https://hdl.handle.net/21.15107/rcub_ibiss_6280 .

Ajdžanović, Vladimir, Šošić-Jurjević, Branka, Filipović, Branko, Popovska-Perčinić, Florina, Jarić, Ivana, Miler, Marko, Ušćebrka, Gordana, Milošević, Verica, "Adrenal zona reticularis in adult orchidectomized male rats after treatment with genistein" in Proceedings: Days of Veterinary Medicine 2016: 7th International Scientific Meeting; 2016 Sep 22-24; Struga, Republic of Macedonia (2016):130-131,
https://hdl.handle.net/21.15107/rcub_ibiss_6280 .

TY  - CONF
AU  - Miler, Marko
AU  - Ajdžanović, Vladimir
AU  - Jarić, Ivana
AU  - Živanović, Jasmina
AU  - Trifunović, Svetlana
AU  - Milošević, Verica
AU  - Šošić-Jurjević, Branka 
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6277
AB  - Age-related decline in liver functioning is associated with increased free radical generation. If free radicals are not rapidly removed, accumulation of damaged products causes cellular senescence. Hesperetin, a flavanone most abundant in citrus fruits, was identified as the promising phyto-therapeutic agent due to its putative anti-oxidative properties. Therefore, the aim of this study was to test whether hesperetin affects liver antioxidant enzymes (AOE), namely, superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) activities, gene and protein expressions in rat ageing model. Twenty-four-month-old rats received per os hesperetin mixed with sunflower oil (vehicle), at a dose of 15 mg/kg, once daily, for 4 weeks. Controls received the same amount of vehicle alone. The mixture was applied to the oral cavity, in order to achieve more nutritionally relevant study. Our results demonstrated that after hesperetin supplementation, activities of CAT and GR were increased by 56% and 28% (p<0.05), respectively. The protein expressions of GPx and SOD2 were significantly decreased by 28% and 51 % (p<0.05), respectively, while just CAT gene expression was increased by 2.9 fold (p<0.05) in comparison with the values obtained for controls. These results suggest that hesperetin treatment affect some AOE in the livers of old-aged rats. It had strongest effect on CAT by increasing its activity and gene expression, without more prominent effect on other evaluated AOE. Decreased or unchanged values obtained for other AOE might be outcome of hesperetin antioxidant and free radical scavenging properties in the livers of rat ageing model.
PB  - Bratislava: Institute of Normal and Pathological Physiology, Slovak Academy of Sciences
C3  - 2nd Joint Meeting of Slovak and Serbian Physiological Societies: Physiology without Frontiers: book of abstracts; 2016 May 15-18; Smolenice, Slovakia
T1  - Hesperetin affects some antioxidative enzymes activity in the liver of old-aged Wistar rats
SP  - 32
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6277
ER  - 

@conference{
author = "Miler, Marko and Ajdžanović, Vladimir and Jarić, Ivana and Živanović, Jasmina and Trifunović, Svetlana and Milošević, Verica and Šošić-Jurjević, Branka ",
year = "2016",
abstract = "Age-related decline in liver functioning is associated with increased free radical generation. If free radicals are not rapidly removed, accumulation of damaged products causes cellular senescence. Hesperetin, a flavanone most abundant in citrus fruits, was identified as the promising phyto-therapeutic agent due to its putative anti-oxidative properties. Therefore, the aim of this study was to test whether hesperetin affects liver antioxidant enzymes (AOE), namely, superoxide dismutase 1 and 2 (SOD 1 and 2), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR) activities, gene and protein expressions in rat ageing model. Twenty-four-month-old rats received per os hesperetin mixed with sunflower oil (vehicle), at a dose of 15 mg/kg, once daily, for 4 weeks. Controls received the same amount of vehicle alone. The mixture was applied to the oral cavity, in order to achieve more nutritionally relevant study. Our results demonstrated that after hesperetin supplementation, activities of CAT and GR were increased by 56% and 28% (p<0.05), respectively. The protein expressions of GPx and SOD2 were significantly decreased by 28% and 51 % (p<0.05), respectively, while just CAT gene expression was increased by 2.9 fold (p<0.05) in comparison with the values obtained for controls. These results suggest that hesperetin treatment affect some AOE in the livers of old-aged rats. It had strongest effect on CAT by increasing its activity and gene expression, without more prominent effect on other evaluated AOE. Decreased or unchanged values obtained for other AOE might be outcome of hesperetin antioxidant and free radical scavenging properties in the livers of rat ageing model.",
publisher = "Bratislava: Institute of Normal and Pathological Physiology, Slovak Academy of Sciences",
journal = "2nd Joint Meeting of Slovak and Serbian Physiological Societies: Physiology without Frontiers: book of abstracts; 2016 May 15-18; Smolenice, Slovakia",
title = "Hesperetin affects some antioxidative enzymes activity in the liver of old-aged Wistar rats",
pages = "32",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6277"
}

Miler, M., Ajdžanović, V., Jarić, I., Živanović, J., Trifunović, S., Milošević, V.,& Šošić-Jurjević, B.. (2016). Hesperetin affects some antioxidative enzymes activity in the liver of old-aged Wistar rats. in 2nd Joint Meeting of Slovak and Serbian Physiological Societies: Physiology without Frontiers: book of abstracts; 2016 May 15-18; Smolenice, Slovakia
Bratislava: Institute of Normal and Pathological Physiology, Slovak Academy of Sciences., 32.
https://hdl.handle.net/21.15107/rcub_ibiss_6277

Miler M, Ajdžanović V, Jarić I, Živanović J, Trifunović S, Milošević V, Šošić-Jurjević B. Hesperetin affects some antioxidative enzymes activity in the liver of old-aged Wistar rats. in 2nd Joint Meeting of Slovak and Serbian Physiological Societies: Physiology without Frontiers: book of abstracts; 2016 May 15-18; Smolenice, Slovakia. 2016;:32.
https://hdl.handle.net/21.15107/rcub_ibiss_6277 .

Miler, Marko, Ajdžanović, Vladimir, Jarić, Ivana, Živanović, Jasmina, Trifunović, Svetlana, Milošević, Verica, Šošić-Jurjević, Branka , "Hesperetin affects some antioxidative enzymes activity in the liver of old-aged Wistar rats" in 2nd Joint Meeting of Slovak and Serbian Physiological Societies: Physiology without Frontiers: book of abstracts; 2016 May 15-18; Smolenice, Slovakia (2016):32,
https://hdl.handle.net/21.15107/rcub_ibiss_6277 .