TY  - JOUR
AU  - Vukašinović, Elvira
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Čelić, Tatjana
AU  - Pihler, Ivan
AU  - Blagojević, Duško
PY  - 2021
UR  - http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100417&tlng=en
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4699
AB  - The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and trans1. Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. Brazilian Arch Biol Technol. 2021;64:e21200773. cription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action.
PB  - Parana, Brazil: Technology Institute of Paraná
T2  - Brazilian Archives of Biology and Technology
T1  - Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut
VL  - 64
DO  - 10.1590/1678-4324-2021200773
SP  - e21200773
ER  - 

@article{
author = "Vukašinović, Elvira and Purać, Jelena and Kojić, Danijela and Čelić, Tatjana and Pihler, Ivan and Blagojević, Duško",
year = "2021",
abstract = "The aim of the present study was to analyze the mechanisms of ibogaine action by measuring its ex vivo effects on antioxidant defense in the honey bee (Apis mellifera, L.) midgut. The transcriptional levels of selected genes: Cu/Zn dependent and Mn dependent superoxide dismutases (Sod1 and Sod2, respectively), catalase (Cat) and trans1. Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. Brazilian Arch Biol Technol. 2021;64:e21200773. cription factor Nrf2 (Nrf2) were determined. The applicability of midgut tissue, which expected to have well developed antioxidant protection system, for this type of analysis was confirmed by testing cell viability and response to paraquat, an effective inducer of oxidative stress, ex vivo. Incubation for 2 h with paraquat (10 µg/mL) induced a significant increase in expression of Sod1 and Cat genes. The results of ibogaine treatment showed that exposure to 5 µg/mL and 10 µg/mL of ibogaine for 2 h induced significant increase in expression of Sod1 gene. On the other hand, ibogaine did not lead to a significant increase of Sod2, Cat and transcription factor Nrf2 genes expression in honey bee midgut ex vivo. Our results confirmed positive effect of ibogaine on the antioxidant protective system and its pro-antioxidant action.",
publisher = "Parana, Brazil: Technology Institute of Paraná",
journal = "Brazilian Archives of Biology and Technology",
title = "Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut",
volume = "64",
doi = "10.1590/1678-4324-2021200773",
pages = "e21200773"
}

Vukašinović, E., Purać, J., Kojić, D., Čelić, T., Pihler, I.,& Blagojević, D.. (2021). Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. in Brazilian Archives of Biology and Technology
Parana, Brazil: Technology Institute of Paraná., 64, e21200773.
https://doi.org/10.1590/1678-4324-2021200773

Vukašinović E, Purać J, Kojić D, Čelić T, Pihler I, Blagojević D. Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut. in Brazilian Archives of Biology and Technology. 2021;64:e21200773.
doi:10.1590/1678-4324-2021200773 .

Vukašinović, Elvira, Purać, Jelena, Kojić, Danijela, Čelić, Tatjana, Pihler, Ivan, Blagojević, Duško, "Ex Vivo Effect of Ibogaine on the Transcriptional Level of Antioxidant Defense Related Genes in Honey Bee (Apis mellifera, L.) Midgut" in Brazilian Archives of Biology and Technology, 64 (2021):e21200773,
https://doi.org/10.1590/1678-4324-2021200773 . .

TY  - JOUR
AU  - Uzelac, Iva
AU  - Avramov, Miloš
AU  - Čelić, Tatjana
AU  - Vukašinović, Elvira
AU  - Gošić-Dondo, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Blagojević, Duško
AU  - Popović, Željko D.
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3706
AB  - The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.
PB  - Nature Research
T2  - Scientific Reports
T1  - Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-65926-w
SP  - 1
EP  - 11
ER  - 

@article{
author = "Uzelac, Iva and Avramov, Miloš and Čelić, Tatjana and Vukašinović, Elvira and Gošić-Dondo, Snežana and Purać, Jelena and Kojić, Danijela and Blagojević, Duško and Popović, Željko D.",
year = "2020",
abstract = "The European corn borer, Ostrinia nubilalis Hbn., is a pest Lepidopteran species whose larvae overwinter by entering diapause, gradually becoming cold-hardy. To investigate metabolic changes during cold hardening, activities of four metabolic enzymes – citrate synthase (CS), lactate dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in whole-body homogenates of pupae, non-diapausing and diapausing larvae acclimated to 5 °C, −3 °C and −16 °C. The highest CS activity was detected in non-diapausing larvae, reflecting active development, while the highest in vitro LDH activity was recorded in diapausing larvae at temperatures close to 0 °C, evidencing a metabolic switch towards anaerobic metabolism. However, in-gel LDH activity showed that production of pyruvate from lactate is triggered by sub-zero temperatures. The activities of both aminotransferases were highest in non-diapausing larvae. Our findings suggest that during diapause and cold hardening the aminotransferases catalyse production of L-alanine, an important cryoprotectant, and L-aspartate, which is closely tied to both transamination reactions and Krebs cycle. The results of this study indicate that, during diapause, the activity of metabolic enzymes is synchronized with exogenous factors, such as temperatures close to 0 °C. These findings support the notion that diapause is metabolically plastic and vibrant, rather than simply a passive, resting state.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-65926-w",
pages = "1-11"
}

Uzelac, I., Avramov, M., Čelić, T., Vukašinović, E., Gošić-Dondo, S., Purać, J., Kojić, D., Blagojević, D.,& Popović, Ž. D.. (2020). Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.). in Scientific Reports
Nature Research., 10(1), 1-11.
https://doi.org/10.1038/s41598-020-65926-w

Uzelac I, Avramov M, Čelić T, Vukašinović E, Gošić-Dondo S, Purać J, Kojić D, Blagojević D, Popović ŽD. Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.). in Scientific Reports. 2020;10(1):1-11.
doi:10.1038/s41598-020-65926-w .

Uzelac, Iva, Avramov, Miloš, Čelić, Tatjana, Vukašinović, Elvira, Gošić-Dondo, Snežana, Purać, Jelena, Kojić, Danijela, Blagojević, Duško, Popović, Željko D., "Effect of Cold Acclimation on Selected Metabolic Enzymes During Diapause in The European Corn Borer Ostrinia nubilalis (Hbn.)" in Scientific Reports, 10, no. 1 (2020):1-11,
https://doi.org/10.1038/s41598-020-65926-w . .

TY  - JOUR
AU  - Nikolić, Tatjana
AU  - Kojić, Danijela
AU  - Orčić, Snežana
AU  - Vukašinović, Elvira
AU  - Blagojević, Duško
AU  - Purać, Jelena
PY  - 2019
UR  - http://link.springer.com/10.1007/s11356-018-3950-6
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3236
AB  - In the present study, the influence of cadmium, copper, and lead on two enzymes often used as biomarkers in toxicological analysis was investigated. Bees were fed with 1 M sucrose solution containing 10-fold serial dilutions of CuCl2 (1000 mg L-1, 100 mg L-1, and 10 mg L-1), CdCl2 (0.1 mg L-1, 0.01 mg L-1, and 0.001 mg L-1), or PbCl2 (10 mg L-1, 1 mg L-1, and 0.1 mg L-1) during 48 h. Our results showed that the total glutathione S-transferase activity was not changed under the influence of cadmium and lead, and it was decreased with the highest concentration of copper. The level of gene expression of the three analyzed classes of glutathione S-transferase was significantly increased with increasing concentrations of copper and cadmium. Lead did not cause significant changes in glutathione S-transferase activity and gene expression, while it showed biphasic effect on acetylcholinesterase activity: lower concentration of lead, 0.1 mg L-1 inhibited and higher dose, 10 mg L-1 induced acetylcholinesterase activity in honey bees. Furthermore, our results showed a significant decrease of the acetylcholinesterase activity in honey bees treated with 0.001 and 0.01 mg L-1 CdCl2. Our results indicate the influence of cadmium, copper, and lead on GST and AChE in the honey bees. These results form the basis for future research on the impact of metallic trace element pollution on honey bees.
T2  - Environmental Science and Pollution Research
T1  - Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.
DO  - 10.1007/s11356-018-3950-6
ER  - 

@article{
author = "Nikolić, Tatjana and Kojić, Danijela and Orčić, Snežana and Vukašinović, Elvira and Blagojević, Duško and Purać, Jelena",
year = "2019",
abstract = "In the present study, the influence of cadmium, copper, and lead on two enzymes often used as biomarkers in toxicological analysis was investigated. Bees were fed with 1 M sucrose solution containing 10-fold serial dilutions of CuCl2 (1000 mg L-1, 100 mg L-1, and 10 mg L-1), CdCl2 (0.1 mg L-1, 0.01 mg L-1, and 0.001 mg L-1), or PbCl2 (10 mg L-1, 1 mg L-1, and 0.1 mg L-1) during 48 h. Our results showed that the total glutathione S-transferase activity was not changed under the influence of cadmium and lead, and it was decreased with the highest concentration of copper. The level of gene expression of the three analyzed classes of glutathione S-transferase was significantly increased with increasing concentrations of copper and cadmium. Lead did not cause significant changes in glutathione S-transferase activity and gene expression, while it showed biphasic effect on acetylcholinesterase activity: lower concentration of lead, 0.1 mg L-1 inhibited and higher dose, 10 mg L-1 induced acetylcholinesterase activity in honey bees. Furthermore, our results showed a significant decrease of the acetylcholinesterase activity in honey bees treated with 0.001 and 0.01 mg L-1 CdCl2. Our results indicate the influence of cadmium, copper, and lead on GST and AChE in the honey bees. These results form the basis for future research on the impact of metallic trace element pollution on honey bees.",
journal = "Environmental Science and Pollution Research",
title = "Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.",
doi = "10.1007/s11356-018-3950-6"
}

Nikolić, T., Kojić, D., Orčić, S., Vukašinović, E., Blagojević, D.,& Purać, J.. (2019). Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.. in Environmental Science and Pollution Research.
https://doi.org/10.1007/s11356-018-3950-6

Nikolić T, Kojić D, Orčić S, Vukašinović E, Blagojević D, Purać J. Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead.. in Environmental Science and Pollution Research. 2019;.
doi:10.1007/s11356-018-3950-6 .

Nikolić, Tatjana, Kojić, Danijela, Orčić, Snežana, Vukašinović, Elvira, Blagojević, Duško, Purać, Jelena, "Laboratory bioassays on the response of honey bee (Apis mellifera L.) glutathione S-transferase and acetylcholinesterase to the oral exposure to copper, cadmium, and lead." in Environmental Science and Pollution Research (2019),
https://doi.org/10.1007/s11356-018-3950-6 . .

TY  - JOUR
AU  - Orčić, Snežana
AU  - Nikolić, Tatjana
AU  - Purać, Jelena
AU  - Šikoparija, Branko
AU  - Blagojević, Duško
AU  - Vukašinović, Elvira
AU  - Plavša, Nada
AU  - Stevanović, Jevrosima
AU  - Kojić, Danijela
PY  - 2017
UR  - http://doi.wiley.com/10.1111/eea.12633
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2964
AB  - The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.
T2  - Entomologia Experimentalis et Applicata
T1  - Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees
IS  - 2-3
VL  - 165
DO  - 10.1111/eea.12633
SP  - 120
EP  - 128
ER  - 

@article{
author = "Orčić, Snežana and Nikolić, Tatjana and Purać, Jelena and Šikoparija, Branko and Blagojević, Duško and Vukašinović, Elvira and Plavša, Nada and Stevanović, Jevrosima and Kojić, Danijela",
year = "2017",
abstract = "The recent decline in managed honey bee populations, Apis mellifera L. (Hymenoptera: Apidae), has caused scientific, ecological, and economic concern. Research into the formation of reactive oxygen species (ROS), antioxidative defense mechanisms, and oxidative stress can contribute to our understanding of bee survival and conservation of this species. Activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) enzymes together with levels of malondialdehyde (MDA) were measured in summer and winter honey bees sampled from three colonies. One colony was stationary (C1), entering the winter period having accumulated Robinia pseudoacacia L. (Fabaceae) honey, and two were migratory (C2 and C3), entering the winter period with mainly Tilia (Malvaceae) and Brassica (Brassicaceae) honey, respectively. Compared to summer workers, winter worker bees had decreased SOD and GST activity, and MDA level, whereas CAT activity increased in all three colonies. We also demonstrated that seasonality is the main factor responsible for changes in antioxidant enzymes and MDA levels in worker honey bees. Overall, our results indicate a difference between summer and winter worker bees, pointing at a reduced level of antioxidant enzyme defenses during overwintering which may be due to a decrease in production of ROS. The decreased levels of MDA measured in winter honey bees confirm this. As ROS are actively used by insects as a defense mechanism to fight pathogens, we suggest that reduced production of ROS contributes to higher susceptibility of winter honey bees to infections and reduced overwinter survival.",
journal = "Entomologia Experimentalis et Applicata",
title = "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees",
number = "2-3",
volume = "165",
doi = "10.1111/eea.12633",
pages = "120-128"
}

Orčić, S., Nikolić, T., Purać, J., Šikoparija, B., Blagojević, D., Vukašinović, E., Plavša, N., Stevanović, J.,& Kojić, D.. (2017). Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata, 165(2-3), 120-128.
https://doi.org/10.1111/eea.12633

Orčić S, Nikolić T, Purać J, Šikoparija B, Blagojević D, Vukašinović E, Plavša N, Stevanović J, Kojić D. Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees. in Entomologia Experimentalis et Applicata. 2017;165(2-3):120-128.
doi:10.1111/eea.12633 .

Orčić, Snežana, Nikolić, Tatjana, Purać, Jelena, Šikoparija, Branko, Blagojević, Duško, Vukašinović, Elvira, Plavša, Nada, Stevanović, Jevrosima, Kojić, Danijela, "Seasonal variation in the activity of selected antioxidant enzymes and malondialdehyde level in worker honey bees" in Entomologia Experimentalis et Applicata, 165, no. 2-3 (2017):120-128,
https://doi.org/10.1111/eea.12633 . .

TY  - JOUR
AU  - Nikolić, Tatjana V.
AU  - Kojić, Danijela
AU  - Orčić, Snežana
AU  - Batinić, Darko
AU  - Vukašinović, Elvira
AU  - Blagojević, Duško
AU  - Purać, Jelena
PY  - 2016
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27580263
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0045653516311006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2611
AB  - In this study, laboratory bioassays were performed to investigate the impact of sublethal concentrations of Cu (CuCl2: 1000, 100, 10 mg L(-1)), Pb (PbCl2: 10, 1, 0.1 mg L(-1)) and Cd (CdCl2: 0.1, 0.01, 0.001 mg L(-1)) on honey bee redox status and the activity of the main antioxidative enzymes and their gene expression. Our results show that exposure to these metals led to significant changes of gene expression, the levels of enzyme activity and redox status, but the effects are metal and dose dependent. In general, exposure of 48 h to given concentrations of Cu, Cd and Pb did not change the activity of antioxidative enzymes and the level of lipid peroxidation, with the exception of decreased activity of catalase at the lowest concentration of cadmium. Only lead produced increases in glutathione and thiol groups. Expression of genes for catalase and superoxide dismutase changed with exposure to cadmium and copper, whilst lead induced only expression of superoxide dismutase genes. The results from this study provide basic data for future research regarding the impacts of metal pollution on Apis mellifera and will be an important step towards a comprehensive risk assessment of the environmental stressors on honey bees.
T2  - Chemosphere
T1  - The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.
VL  - 164
DO  - 10.1016/j.chemosphere.2016.08.077
SP  - 98
EP  - 105
ER  - 

@article{
author = "Nikolić, Tatjana V. and Kojić, Danijela and Orčić, Snežana and Batinić, Darko and Vukašinović, Elvira and Blagojević, Duško and Purać, Jelena",
year = "2016",
abstract = "In this study, laboratory bioassays were performed to investigate the impact of sublethal concentrations of Cu (CuCl2: 1000, 100, 10 mg L(-1)), Pb (PbCl2: 10, 1, 0.1 mg L(-1)) and Cd (CdCl2: 0.1, 0.01, 0.001 mg L(-1)) on honey bee redox status and the activity of the main antioxidative enzymes and their gene expression. Our results show that exposure to these metals led to significant changes of gene expression, the levels of enzyme activity and redox status, but the effects are metal and dose dependent. In general, exposure of 48 h to given concentrations of Cu, Cd and Pb did not change the activity of antioxidative enzymes and the level of lipid peroxidation, with the exception of decreased activity of catalase at the lowest concentration of cadmium. Only lead produced increases in glutathione and thiol groups. Expression of genes for catalase and superoxide dismutase changed with exposure to cadmium and copper, whilst lead induced only expression of superoxide dismutase genes. The results from this study provide basic data for future research regarding the impacts of metal pollution on Apis mellifera and will be an important step towards a comprehensive risk assessment of the environmental stressors on honey bees.",
journal = "Chemosphere",
title = "The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.",
volume = "164",
doi = "10.1016/j.chemosphere.2016.08.077",
pages = "98-105"
}

Nikolić, T. V., Kojić, D., Orčić, S., Batinić, D., Vukašinović, E., Blagojević, D.,& Purać, J.. (2016). The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.. in Chemosphere, 164, 98-105.
https://doi.org/10.1016/j.chemosphere.2016.08.077

Nikolić TV, Kojić D, Orčić S, Batinić D, Vukašinović E, Blagojević D, Purać J. The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions.. in Chemosphere. 2016;164:98-105.
doi:10.1016/j.chemosphere.2016.08.077 .

Nikolić, Tatjana V., Kojić, Danijela, Orčić, Snežana, Batinić, Darko, Vukašinović, Elvira, Blagojević, Duško, Purać, Jelena, "The impact of sublethal concentrations of Cu, Pb and Cd on honey bee redox status, superoxide dismutase and catalase in laboratory conditions." in Chemosphere, 164 (2016):98-105,
https://doi.org/10.1016/j.chemosphere.2016.08.077 . .

TY  - JOUR
AU  - Vukašinović, Elvira
AU  - Pond, David
AU  - Worland, Michael Roger
AU  - Kojić, Danijela
AU  - Purać, Jelena
AU  - Blagojević, Duško
AU  - Grubor-Lajšić, Gordana
PY  - 2013
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6337
AB  - This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
T1  - Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)
IS  - 4
VL  - 165
DO  - 10.1016/j.cbpb.2013.05.001
SP  - 219
EP  - 225
ER  - 

@article{
author = "Vukašinović, Elvira and Pond, David and Worland, Michael Roger and Kojić, Danijela and Purać, Jelena and Blagojević, Duško and Grubor-Lajšić, Gordana",
year = "2013",
abstract = "This study compares the composition and biophysical properties of lipids in non-diapausing and diapausing
fifth instar larvae of Ostrinia nubilalis Hubn. (Lepidoptera: Crambidae). The majority of fat body lipids in both of these physiological states were comprised of ~90% triacylglycerols (TAGs), whereas the haemolymph contained a more even distribution of all lipid classes. The fatty acid composition and biophysical properties of the fat body lipids differed markedly between non-diapausing and diapausing larvae. Diapause was associated with a dramatic increase in the proportions of palmitoleic acid (16:1n-7) and oleic acid (18:1n-9), with concurrent reductions in palmitic acid (16:0) and linoleic acid (18:2n-6). The increase in the level of unsaturation of the fat body lipids, which caused a marked shift in their phase transitions to lower temperatures, was triggered by diapause rather than low temperatures. Adjustments of fatty acid compositions are likely to be an important component of winter diapause mechanisms, possibly maintaining the fluidity of cell membranes and the functionality of the organism during lower winter temperatures.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology",
title = "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)",
number = "4",
volume = "165",
doi = "10.1016/j.cbpb.2013.05.001",
pages = "219-225"
}

Vukašinović, E., Pond, D., Worland, M. R., Kojić, D., Purać, J., Blagojević, D.,& Grubor-Lajšić, G.. (2013). Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
Elsevier Inc.., 165(4), 219-225.
https://doi.org/10.1016/j.cbpb.2013.05.001

Vukašinović E, Pond D, Worland MR, Kojić D, Purać J, Blagojević D, Grubor-Lajšić G. Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae). in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 2013;165(4):219-225.
doi:10.1016/j.cbpb.2013.05.001 .

Vukašinović, Elvira, Pond, David, Worland, Michael Roger, Kojić, Danijela, Purać, Jelena, Blagojević, Duško, Grubor-Lajšić, Gordana, "Diapause induces changes in the composition and biophysical properties of lipids in larvae of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae)" in Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 165, no. 4 (2013):219-225,
https://doi.org/10.1016/j.cbpb.2013.05.001 . .