TY  - DATA
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Jović, Jelena
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Đorđević, Mirko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6696
AB  - Data sets for life history traits (early fecundity, total fecundity, lifespan and fertility) of female and male seed beetles (Acanthoscelides obtectus) harbouring one of the three naturally occurring mitochondrial haplotypes (MG1a, MG1d and MG3b).
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - TFT life history
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6696
ER  - 

@misc{
author = "Vlajnić, Lea and Savković, Uroš and Jović, Jelena and Budečević, Sanja and Stojković, Biljana and Đorđević, Mirko",
year = "2024",
abstract = "Data sets for life history traits (early fecundity, total fecundity, lifespan and fertility) of female and male seed beetles (Acanthoscelides obtectus) harbouring one of the three naturally occurring mitochondrial haplotypes (MG1a, MG1d and MG3b).",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "TFT life history",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6696"
}

Vlajnić, L., Savković, U., Jović, J., Budečević, S., Stojković, B.,& Đorđević, M.. (2024). TFT life history. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6696

Vlajnić L, Savković U, Jović J, Budečević S, Stojković B, Đorđević M. TFT life history. 2024;.
https://hdl.handle.net/21.15107/rcub_ibiss_6696 .

Vlajnić, Lea, Savković, Uroš, Jović, Jelena, Budečević, Sanja, Stojković, Biljana, Đorđević, Mirko, "TFT life history" (2024),
https://hdl.handle.net/21.15107/rcub_ibiss_6696 .

TY  - JOUR
AU  - Rončević, Aleksa
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
AU  - Budečević, Sanja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6694
AB  - Morphological plasticity may be induced by altering host plants and commonly it is sexspecific in phytophagous insects. It is hypothesized that stress in insects caused by a host shift
leads to morphological changes and developmental destabilization, which may be identified
by fluctuating asymmetry. We performed reciprocal transplant experiments in eight replicated
populations of the seed beetle Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae,
Bruchinae) adapted to common bean (Phaseolus vulgaris L., Fabaceae) as its ancestral host
and chickpea (Cicer arietinum L., Fabaceae) as a suboptimal host. Using methods of
geometric morphometrics we assessed the effects of the short- and long-term host shift on size
and shape variation of beetles of both sexes. We also tested the hypothesis that fluctuating
asymmetry is lower when the beetles develop within seeds of the ancestral host. Our results
showed that females respond more plastically related to their body size and shape of the
abdomen, particularly during a short-term host shift, suggesting that females have a greater
importance in maintaining the population on a new host. The level of fluctuating asymmetry
is lowest in males that have evolved for generations on bean indicating that they have the
most canalized development, so we suggested that symmetry in A. obtectus is maintained by
sexual selection. Even if the beetles are adapted to a suboptimal host over many generations,
they stabilize their development after returning to the ancestral host. Our work indicates that
host shifts may change morphological aspects of the beetles in a sex-specific manner and
consequently influence their developmental trajectories.
PB  - John Wiley & Sons Ltd
T2  - Entomologia Experimentalis at Applicata
T1  - Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach
DO  - 10.1111/eea.13453
ER  - 

@article{
author = "Rončević, Aleksa and Savković, Uroš and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana and Budečević, Sanja",
year = "2024",
abstract = "Morphological plasticity may be induced by altering host plants and commonly it is sexspecific in phytophagous insects. It is hypothesized that stress in insects caused by a host shift
leads to morphological changes and developmental destabilization, which may be identified
by fluctuating asymmetry. We performed reciprocal transplant experiments in eight replicated
populations of the seed beetle Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae,
Bruchinae) adapted to common bean (Phaseolus vulgaris L., Fabaceae) as its ancestral host
and chickpea (Cicer arietinum L., Fabaceae) as a suboptimal host. Using methods of
geometric morphometrics we assessed the effects of the short- and long-term host shift on size
and shape variation of beetles of both sexes. We also tested the hypothesis that fluctuating
asymmetry is lower when the beetles develop within seeds of the ancestral host. Our results
showed that females respond more plastically related to their body size and shape of the
abdomen, particularly during a short-term host shift, suggesting that females have a greater
importance in maintaining the population on a new host. The level of fluctuating asymmetry
is lowest in males that have evolved for generations on bean indicating that they have the
most canalized development, so we suggested that symmetry in A. obtectus is maintained by
sexual selection. Even if the beetles are adapted to a suboptimal host over many generations,
they stabilize their development after returning to the ancestral host. Our work indicates that
host shifts may change morphological aspects of the beetles in a sex-specific manner and
consequently influence their developmental trajectories.",
publisher = "John Wiley & Sons Ltd",
journal = "Entomologia Experimentalis at Applicata",
title = "Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach",
doi = "10.1111/eea.13453"
}

Rončević, A., Savković, U., Đorđević, M., Vlajnić, L., Stojković, B.,& Budečević, S.. (2024). Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach. in Entomologia Experimentalis at Applicata
John Wiley & Sons Ltd..
https://doi.org/10.1111/eea.13453

Rončević A, Savković U, Đorđević M, Vlajnić L, Stojković B, Budečević S. Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach. in Entomologia Experimentalis at Applicata. 2024;.
doi:10.1111/eea.13453 .

Rončević, Aleksa, Savković, Uroš, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, Budečević, Sanja, "Sex-specific consequences of host shift for morphology and fluctuating asymmetry in a seed beetle: an experimental evolution approach" in Entomologia Experimentalis at Applicata (2024),
https://doi.org/10.1111/eea.13453 . .

TY  - CONF
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Pavlović, Kasja
AU  - Krako Jakoviljević, Nina
AU  - Ivanović, Tijana
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Đorđević, Mirko
PY  - 2023
UR  - https://ece2023.com/scientific-program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6252
AB  - Evolutionary theory suggests that inheritance of mitochondria through the maternal lineage could drive the accumulation of male-harming mutations in mitochondrial genomes. Namely, due to uniparental mode of mitochondrial inheritance, natural selection is “blind” to mutations that are deleterious to males as long as they are beneficial or neutral to females. This evolutionary phenomenon is known as ‘the Mother’s Curse’. The male-specific adverse effects of mitochondrial mutations are thought to be due to the fact that males are sensitive to impairments in the function of the oxidative phosphorylation (OXPHOS) system, i.e. mitochondrial respiration. The OXPHOS system consists of five complexes whose subunits are coded by the mitochondrial and nuclear genomes. In our previous work, we identified several mitochondrial haplotypes (mitotypes) in laboratory populations of the seed beetle (Acanthoscelides obtectus). One of them, MG3b mitotype, significantly reduces male fertility compared to other mitotypes when expressed in the same nuclear environment, but has no negative effects on females. In this study, we tested whether the MG3b mitotype affects OXPHOS functioning in males compared with other, control mitotypes. Specifically, we expressed MG3b and two control mitotypes alongside the same nuclear background and measured mitochondrial respiration in males using high-resolution respirometry. Our results show that respiration linked to all OXPHOS complexes is significantly reduced in MG3b males compared with controls. This is particularly true for the complex IV, which shows mean reduction in activity of around 40%. This work provides evidence that the decrease in mitochondrial respiration is the cause of subfertility of MG3b males.
PB  - Hellenic Entomological Society
C3  - Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
T1  - Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility
SP  - 40
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6252
ER  - 

@conference{
author = "Vlajnić, Lea and Savković, Uroš and Pavlović, Kasja and Krako Jakoviljević, Nina and Ivanović, Tijana and Budečević, Sanja and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Stojković, Oliver and Đorđević, Mirko",
year = "2023",
abstract = "Evolutionary theory suggests that inheritance of mitochondria through the maternal lineage could drive the accumulation of male-harming mutations in mitochondrial genomes. Namely, due to uniparental mode of mitochondrial inheritance, natural selection is “blind” to mutations that are deleterious to males as long as they are beneficial or neutral to females. This evolutionary phenomenon is known as ‘the Mother’s Curse’. The male-specific adverse effects of mitochondrial mutations are thought to be due to the fact that males are sensitive to impairments in the function of the oxidative phosphorylation (OXPHOS) system, i.e. mitochondrial respiration. The OXPHOS system consists of five complexes whose subunits are coded by the mitochondrial and nuclear genomes. In our previous work, we identified several mitochondrial haplotypes (mitotypes) in laboratory populations of the seed beetle (Acanthoscelides obtectus). One of them, MG3b mitotype, significantly reduces male fertility compared to other mitotypes when expressed in the same nuclear environment, but has no negative effects on females. In this study, we tested whether the MG3b mitotype affects OXPHOS functioning in males compared with other, control mitotypes. Specifically, we expressed MG3b and two control mitotypes alongside the same nuclear background and measured mitochondrial respiration in males using high-resolution respirometry. Our results show that respiration linked to all OXPHOS complexes is significantly reduced in MG3b males compared with controls. This is particularly true for the complex IV, which shows mean reduction in activity of around 40%. This work provides evidence that the decrease in mitochondrial respiration is the cause of subfertility of MG3b males.",
publisher = "Hellenic Entomological Society",
journal = "Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece",
title = "Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility",
pages = "40",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6252"
}

Vlajnić, L., Savković, U., Pavlović, K., Krako Jakoviljević, N., Ivanović, T., Budečević, S., Stojković, B., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A., Stojković, O.,& Đorđević, M.. (2023). Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
Hellenic Entomological Society., 40.
https://hdl.handle.net/21.15107/rcub_ibiss_6252

Vlajnić L, Savković U, Pavlović K, Krako Jakoviljević N, Ivanović T, Budečević S, Stojković B, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Stojković O, Đorđević M. Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece. 2023;:40.
https://hdl.handle.net/21.15107/rcub_ibiss_6252 .

Vlajnić, Lea, Savković, Uroš, Pavlović, Kasja, Krako Jakoviljević, Nina, Ivanović, Tijana, Budečević, Sanja, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Stojković, Oliver, Đorđević, Mirko, "Mitochondrial genotype decreases mitochondrial respiration in seed beetles: implications for male subfertility" in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece (2023):40,
https://hdl.handle.net/21.15107/rcub_ibiss_6252 .

TY  - CONF
AU  - Budečević, Sanja
AU  - Rončević, Aleksa
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Savković, Uroš
PY  - 2023
UR  - https://ece2023.com/scientific-program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6254
AB  - Host-shift induced stress in phytophagous insects could have effect on developmental destabilization. Indicator of developmental instability is fluctuating asymmetry (FA)- small random deviations between right and left side of bilateral symmetrical traits. In theory, symmetry is subject to sexual selection in insects, since FA is considered as an indicator of male quality. Response to environmental variation is commonly sex-specific and can affect patterns of sexual dimorphism. We performed reciprocal transplant experiment in eight replicated seed beetle (Acanthoscelides obtectus) populations adapted to bean and chickpea. Within the framework of experimental evolution and using the method of geometric morphometrics we: 1. observed the effects of long and short term host-shift on patterns of shape variation of beetles of both sexes and 2. tested hypothesis that both sexes will have a higher level of FA after long or short term change from optimal to suboptimal host. We found that long term host-shift process affected shape in a way that bean adapted beetles had wider and shorter abdomen compared to chickpea adapted ones. Short-term effects are more notable in bean selected beetles, especially in the abdominal region. Bean adapted males had the most canalized development, i.e. the lowest level of FA, compared to chickpea beetles. In chickpea adapted females, difference in level of FA significantly decreased after the short term host-shift. Our work indicates that host-shift process can change morphological aspects of beetles in a sex-specific manner and consequently influence their developmental trajectories.
PB  - Hellenic Entomological Society
C3  - Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
T1  - Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach
SP  - 362
EP  - 363
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6254
ER  - 

@conference{
author = "Budečević, Sanja and Rončević, Aleksa and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Stojković, Oliver and Savković, Uroš",
year = "2023",
abstract = "Host-shift induced stress in phytophagous insects could have effect on developmental destabilization. Indicator of developmental instability is fluctuating asymmetry (FA)- small random deviations between right and left side of bilateral symmetrical traits. In theory, symmetry is subject to sexual selection in insects, since FA is considered as an indicator of male quality. Response to environmental variation is commonly sex-specific and can affect patterns of sexual dimorphism. We performed reciprocal transplant experiment in eight replicated seed beetle (Acanthoscelides obtectus) populations adapted to bean and chickpea. Within the framework of experimental evolution and using the method of geometric morphometrics we: 1. observed the effects of long and short term host-shift on patterns of shape variation of beetles of both sexes and 2. tested hypothesis that both sexes will have a higher level of FA after long or short term change from optimal to suboptimal host. We found that long term host-shift process affected shape in a way that bean adapted beetles had wider and shorter abdomen compared to chickpea adapted ones. Short-term effects are more notable in bean selected beetles, especially in the abdominal region. Bean adapted males had the most canalized development, i.e. the lowest level of FA, compared to chickpea beetles. In chickpea adapted females, difference in level of FA significantly decreased after the short term host-shift. Our work indicates that host-shift process can change morphological aspects of beetles in a sex-specific manner and consequently influence their developmental trajectories.",
publisher = "Hellenic Entomological Society",
journal = "Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece",
title = "Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach",
pages = "362-363",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6254"
}

Budečević, S., Rončević, A., Đorđević, M., Vlajnić, L., Stojković, B., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A., Stojković, O.,& Savković, U.. (2023). Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
Hellenic Entomological Society., 362-363.
https://hdl.handle.net/21.15107/rcub_ibiss_6254

Budečević S, Rončević A, Đorđević M, Vlajnić L, Stojković B, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Stojković O, Savković U. Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece. 2023;:362-363.
https://hdl.handle.net/21.15107/rcub_ibiss_6254 .

Budečević, Sanja, Rončević, Aleksa, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Stojković, Oliver, Savković, Uroš, "Experimentally induced host-shift changes morphology and fluctuating asymmetry in sex-specific manner in a seed beetle: an experimental evolution approach" in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece (2023):362-363,
https://hdl.handle.net/21.15107/rcub_ibiss_6254 .

TY  - CONF
AU  - Predojević, Dragana
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Budečević, Sanja
PY  - 2023
UR  - https://ece2023.com/scientific-program/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6253
AB  - Insecticide resistance is an example of rapid evolution under strong selection pressure and, is therefore of great importance for human and environmental health and economy. Acanthoscelides obtectus is a globally distributed Coleoptera pest that attacks bean seeds and degrades their quality. One method of protecting against seed beetles is the application of a widely used group of pesticides- pyrethroids. Insecticides, as xenobiotics, can induce stress in pests, leading to destabilization of their development and changes in their morphology. Fluctuating asymmetry (FA), small random deviations between right and left sides of bilateral symmetrical traits, is considered as a result of developmental instability and is thus a measurable indicator of phenotypic response to stress during morphogenesis in insects. In this work, we exposed four populations of A. obtectus to sub-lethal doses (LD20) of pyrethroid insecticide for five generations in a laboratory evolution experiment. To estimate the level of stress, we monitored the effects of the pesticide on shape changes and the level of FA using a geometric morphometric approach. Our results showed that beetles treated with LD20 significantly changed their morphology in the thorax region, while the effect on the level of FA was minor. This work uses experimental evolution as a powerful tool to study pesticide resistance and paves the way for further studies on morphological adaptations, modularity and developmental stability under stress conditions.
PB  - Hellenic Entomological Society
C3  - Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
T1  - Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development
SP  - 365
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6253
ER  - 

@conference{
author = "Predojević, Dragana and Savković, Uroš and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Mitrovski Bogdanović, Ana and Stojković, Oliver and Budečević, Sanja",
year = "2023",
abstract = "Insecticide resistance is an example of rapid evolution under strong selection pressure and, is therefore of great importance for human and environmental health and economy. Acanthoscelides obtectus is a globally distributed Coleoptera pest that attacks bean seeds and degrades their quality. One method of protecting against seed beetles is the application of a widely used group of pesticides- pyrethroids. Insecticides, as xenobiotics, can induce stress in pests, leading to destabilization of their development and changes in their morphology. Fluctuating asymmetry (FA), small random deviations between right and left sides of bilateral symmetrical traits, is considered as a result of developmental instability and is thus a measurable indicator of phenotypic response to stress during morphogenesis in insects. In this work, we exposed four populations of A. obtectus to sub-lethal doses (LD20) of pyrethroid insecticide for five generations in a laboratory evolution experiment. To estimate the level of stress, we monitored the effects of the pesticide on shape changes and the level of FA using a geometric morphometric approach. Our results showed that beetles treated with LD20 significantly changed their morphology in the thorax region, while the effect on the level of FA was minor. This work uses experimental evolution as a powerful tool to study pesticide resistance and paves the way for further studies on morphological adaptations, modularity and developmental stability under stress conditions.",
publisher = "Hellenic Entomological Society",
journal = "Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece",
title = "Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development",
pages = "365",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6253"
}

Predojević, D., Savković, U., Đorđević, M., Vlajnić, L., Stojković, B., Pešić, S., Vukajlović, F., Mitrovski Bogdanović, A., Stojković, O.,& Budečević, S.. (2023). Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece
Hellenic Entomological Society., 365.
https://hdl.handle.net/21.15107/rcub_ibiss_6253

Predojević D, Savković U, Đorđević M, Vlajnić L, Stojković B, Pešić S, Vukajlović F, Mitrovski Bogdanović A, Stojković O, Budečević S. Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development. in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece. 2023;:365.
https://hdl.handle.net/21.15107/rcub_ibiss_6253 .

Predojević, Dragana, Savković, Uroš, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Mitrovski Bogdanović, Ana, Stojković, Oliver, Budečević, Sanja, "Evolution of pesticide resistance in seed beetles: transgenerational effects of sub-lethal pyrethroid concentration on morphology and development" in Book of abstracts: 12th European Congress of Entomology: ECE 2023 Crete; 2023 Oct 16-20; Heraklion, Greece (2023):365,
https://hdl.handle.net/21.15107/rcub_ibiss_6253 .

TY  - DATA
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Vukajlović, Filip
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Stojković, Oliver
AU  - Pešić, Snežana
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Đorđević, Mirko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6017
AB  - This data set is a milestone  of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains raw data on the effects of candidate Trojan female technique (TFT) mutations across the range of nuclear backgrounds on fertility of male and female seed beetles. For obtaining this data set we have used controlled genetic crosses between females with 3 specific mitotypes (1 of which is a candidate TFT mitotype, and 2 are controls) and males with 5 different nuclear backgrounds. Such crossings were used to assess female and male fertility of seed beetles with different combinations of mito-nuclear genomes.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6017
ER  - 

@misc{
author = "Vlajnić, Lea and Savković, Uroš and Vukajlović, Filip and Budečević, Sanja and Stojković, Biljana and Stojković, Oliver and Pešić, Snežana and Predojević, Dragana and Mitrovski Bogdanović, Ana and Đorđević, Mirko",
year = "2023",
abstract = "This data set is a milestone  of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains raw data on the effects of candidate Trojan female technique (TFT) mutations across the range of nuclear backgrounds on fertility of male and female seed beetles. For obtaining this data set we have used controlled genetic crosses between females with 3 specific mitotypes (1 of which is a candidate TFT mitotype, and 2 are controls) and males with 5 different nuclear backgrounds. Such crossings were used to assess female and male fertility of seed beetles with different combinations of mito-nuclear genomes.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6017"
}

Vlajnić, L., Savković, U., Vukajlović, F., Budečević, S., Stojković, B., Stojković, O., Pešić, S., Predojević, D., Mitrovski Bogdanović, A.,& Đorđević, M.. (2023). Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6017

Vlajnić L, Savković U, Vukajlović F, Budečević S, Stojković B, Stojković O, Pešić S, Predojević D, Mitrovski Bogdanović A, Đorđević M. Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6017 .

Vlajnić, Lea, Savković, Uroš, Vukajlović, Filip, Budečević, Sanja, Stojković, Biljana, Stojković, Oliver, Pešić, Snežana, Predojević, Dragana, Mitrovski Bogdanović, Ana, Đorđević, Mirko, "Milestone for the ELEVATE project: Data on the effects of candidate TFT mutations across the range of nuclear backgrounds on life-history traits" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6017 .

TY  - DATA
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Vukajlović, Filip
AU  - Budečević, Sanja
AU  - Stojković, Biljana
AU  - Stojković, Oliver
AU  - Pešić, Snežana
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Đorđević, Mirko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6018
AB  - This data set is a milestone of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains sequences of subunit 1 of cytochrome oxidase (COI) for populations of seed beetles (Acanthoscelides obtectus) with different shares of Trojan female technique (TFT) and control mytotypes.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
T1  - Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6018
ER  - 

@misc{
author = "Vlajnić, Lea and Savković, Uroš and Vukajlović, Filip and Budečević, Sanja and Stojković, Biljana and Stojković, Oliver and Pešić, Snežana and Predojević, Dragana and Mitrovski Bogdanović, Ana and Đorđević, Mirko",
year = "2023",
abstract = "This data set is a milestone of the WP3 work package of the Experimental evolution approach in developing insect pest control methods (ELEVATE) project number #7683961 financed by the Science Fund of the Republic of Serbia. Data set contains sequences of subunit 1 of cytochrome oxidase (COI) for populations of seed beetles (Acanthoscelides obtectus) with different shares of Trojan female technique (TFT) and control mytotypes.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
title = "Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6018"
}

Vlajnić, L., Savković, U., Vukajlović, F., Budečević, S., Stojković, B., Stojković, O., Pešić, S., Predojević, D., Mitrovski Bogdanović, A.,& Đorđević, M.. (2023). Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females. 
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade..
https://hdl.handle.net/21.15107/rcub_ibiss_6018

Vlajnić L, Savković U, Vukajlović F, Budečević S, Stojković B, Stojković O, Pešić S, Predojević D, Mitrovski Bogdanović A, Đorđević M. Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females. 2023;.
https://hdl.handle.net/21.15107/rcub_ibiss_6018 .

Vlajnić, Lea, Savković, Uroš, Vukajlović, Filip, Budečević, Sanja, Stojković, Biljana, Stojković, Oliver, Pešić, Snežana, Predojević, Dragana, Mitrovski Bogdanović, Ana, Đorđević, Mirko, "Milestone for the ELEVATE project: Data on changes in genetic structure of populations relative to starting frequencies of TFT females" (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6018 .

TY  - JOUR
AU  - Immonen, Elina
AU  - Sayadi, Ahmed
AU  - Stojković, Biljana
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Liljestrand-Rönn, Johanna
AU  - Wiberg, Axel
AU  - Arnqvist, Göran
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5378
AB  - The patterns of reproductive timing and senescence vary within and across species owing to differences in reproductive strategies, but our understanding of the molecular underpinnings of such variation is incomplete. This is perhaps particularly true for sex differences. We investigated the evolution of sex-specific gene expression associated with life history divergence in replicated populations of the seed beetle Acanthoscelides obtectus, experimentally evolving under (E)arly or (L)ate life reproduction for >200 generations which has resulted in strongly divergent life histories. We detected 1,646 genes that were differentially expressed in E and L lines, consistent with a highly polygenic basis of life history evolution. Only 30% of differentially expressed genes were similarly affected in males and females. The evolution of long life was associated with significantly reduced sex differences in expression, especially in non-reproductive tissues. The expression differences were overall more pronounced in females, in accordance with their greater phenotypic divergence in lifespan. Functional enrichment analysis revealed differences between E and L beetles in gene categories previously implicated in aging, such as mitochondrial function and defense response. The results show that divergent life history evolution can be associated with profound changes in gene expression that alter the transcriptome in a sex-specific way, highlighting the importance of understanding the mechanisms of aging in each sex.
PB  - Oxford, UK: Oxford University Press
T2  - Genome Biology and Evolution
T1  - Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles
IS  - 1
VL  - 15
DO  - 10.1093/gbe/evac177
SP  - evac177
ER  - 

@article{
author = "Immonen, Elina and Sayadi, Ahmed and Stojković, Biljana and Savković, Uroš and Đorđević, Mirko and Liljestrand-Rönn, Johanna and Wiberg, Axel and Arnqvist, Göran",
year = "2023",
abstract = "The patterns of reproductive timing and senescence vary within and across species owing to differences in reproductive strategies, but our understanding of the molecular underpinnings of such variation is incomplete. This is perhaps particularly true for sex differences. We investigated the evolution of sex-specific gene expression associated with life history divergence in replicated populations of the seed beetle Acanthoscelides obtectus, experimentally evolving under (E)arly or (L)ate life reproduction for >200 generations which has resulted in strongly divergent life histories. We detected 1,646 genes that were differentially expressed in E and L lines, consistent with a highly polygenic basis of life history evolution. Only 30% of differentially expressed genes were similarly affected in males and females. The evolution of long life was associated with significantly reduced sex differences in expression, especially in non-reproductive tissues. The expression differences were overall more pronounced in females, in accordance with their greater phenotypic divergence in lifespan. Functional enrichment analysis revealed differences between E and L beetles in gene categories previously implicated in aging, such as mitochondrial function and defense response. The results show that divergent life history evolution can be associated with profound changes in gene expression that alter the transcriptome in a sex-specific way, highlighting the importance of understanding the mechanisms of aging in each sex.",
publisher = "Oxford, UK: Oxford University Press",
journal = "Genome Biology and Evolution",
title = "Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles",
number = "1",
volume = "15",
doi = "10.1093/gbe/evac177",
pages = "evac177"
}

Immonen, E., Sayadi, A., Stojković, B., Savković, U., Đorđević, M., Liljestrand-Rönn, J., Wiberg, A.,& Arnqvist, G.. (2023). Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles. in Genome Biology and Evolution
Oxford, UK: Oxford University Press., 15(1), evac177.
https://doi.org/10.1093/gbe/evac177

Immonen E, Sayadi A, Stojković B, Savković U, Đorđević M, Liljestrand-Rönn J, Wiberg A, Arnqvist G. Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles. in Genome Biology and Evolution. 2023;15(1):evac177.
doi:10.1093/gbe/evac177 .

Immonen, Elina, Sayadi, Ahmed, Stojković, Biljana, Savković, Uroš, Đorđević, Mirko, Liljestrand-Rönn, Johanna, Wiberg, Axel, Arnqvist, Göran, "Experimental life history evolution results in sex-specific evolution of gene expression in seed beetles" in Genome Biology and Evolution, 15, no. 1 (2023):evac177,
https://doi.org/10.1093/gbe/evac177 . .

TY  - CONF
AU  - Mirčić, Dejan
AU  - Stojković, Biljana
AU  - Ilijin, Larisa
AU  - Mrdaković, Marija
AU  - Vlahović, Milena
AU  - Todorović, Dajana
AU  - Perić Mataruga, Vesna
AU  - Lazarević, Jelica
PY  - 2022
UR  - https://www.hbd-sbc.hr/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5619
AB  - Natural selection has significant effect on phenotypic changes in organisms exposed to chronic environmental stress induced by pollutants such as cadmium. The adaptation to such a stress comes as a direct result of natural selection in traits that will induce the evolution of tolerance to stressful environment both directly and indirectly. A statistic method was used in this experiment to determine the standard linear selection gradients (β’) and standard linear selection differentials (i’) used to estimate the intensity of the natural selection to certain traits of the adaptive values (LD – larval development; PM – pupae mass; PD – pupae development) in control group (C) and treatments ((T1 – 10 μg/g Cd; T2 – 30 μg/g Cd; iT3 – 50 μg/g Cd) where adults lifespan (AL) was considered to be the measure of the adaptive value. Significant negative selection gradients and differencials in males to LD in group T3 were determined in our experiment, as well as positive selection gradients and differentials in females to PM in groups C, T1 and T2. Females showed significant selection gradients and differentials to PM in grpoups C, T1 and T2 while the same occured with males from group C. Positive selection gradients and differencials to PD were determined in females from group T1 and males from groups C and T2. When exposed to stressful enviroments, larger females and males with shorter larval and pupal development seem to be favoured by natural selection.
PB  - Zagreb: Croatian Biological Society
C3  - Book of abstracts: 14th Croatian Biological Congress With International Participation; 2022 Oct 12-16; Pula, Croatia
T1  - Effects of cadmium on natural selection in gypsy moth (Lymantria dispar L.)
SP  - 178
EP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5619
ER  - 

@conference{
author = "Mirčić, Dejan and Stojković, Biljana and Ilijin, Larisa and Mrdaković, Marija and Vlahović, Milena and Todorović, Dajana and Perić Mataruga, Vesna and Lazarević, Jelica",
year = "2022",
abstract = "Natural selection has significant effect on phenotypic changes in organisms exposed to chronic environmental stress induced by pollutants such as cadmium. The adaptation to such a stress comes as a direct result of natural selection in traits that will induce the evolution of tolerance to stressful environment both directly and indirectly. A statistic method was used in this experiment to determine the standard linear selection gradients (β’) and standard linear selection differentials (i’) used to estimate the intensity of the natural selection to certain traits of the adaptive values (LD – larval development; PM – pupae mass; PD – pupae development) in control group (C) and treatments ((T1 – 10 μg/g Cd; T2 – 30 μg/g Cd; iT3 – 50 μg/g Cd) where adults lifespan (AL) was considered to be the measure of the adaptive value. Significant negative selection gradients and differencials in males to LD in group T3 were determined in our experiment, as well as positive selection gradients and differentials in females to PM in groups C, T1 and T2. Females showed significant selection gradients and differentials to PM in grpoups C, T1 and T2 while the same occured with males from group C. Positive selection gradients and differencials to PD were determined in females from group T1 and males from groups C and T2. When exposed to stressful enviroments, larger females and males with shorter larval and pupal development seem to be favoured by natural selection.",
publisher = "Zagreb: Croatian Biological Society",
journal = "Book of abstracts: 14th Croatian Biological Congress With International Participation; 2022 Oct 12-16; Pula, Croatia",
title = "Effects of cadmium on natural selection in gypsy moth (Lymantria dispar L.)",
pages = "178-177",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5619"
}

Mirčić, D., Stojković, B., Ilijin, L., Mrdaković, M., Vlahović, M., Todorović, D., Perić Mataruga, V.,& Lazarević, J.. (2022). Effects of cadmium on natural selection in gypsy moth (Lymantria dispar L.). in Book of abstracts: 14th Croatian Biological Congress With International Participation; 2022 Oct 12-16; Pula, Croatia
Zagreb: Croatian Biological Society., 178-177.
https://hdl.handle.net/21.15107/rcub_ibiss_5619

Mirčić D, Stojković B, Ilijin L, Mrdaković M, Vlahović M, Todorović D, Perić Mataruga V, Lazarević J. Effects of cadmium on natural selection in gypsy moth (Lymantria dispar L.). in Book of abstracts: 14th Croatian Biological Congress With International Participation; 2022 Oct 12-16; Pula, Croatia. 2022;:178-177.
https://hdl.handle.net/21.15107/rcub_ibiss_5619 .

Mirčić, Dejan, Stojković, Biljana, Ilijin, Larisa, Mrdaković, Marija, Vlahović, Milena, Todorović, Dajana, Perić Mataruga, Vesna, Lazarević, Jelica, "Effects of cadmium on natural selection in gypsy moth (Lymantria dispar L.)" in Book of abstracts: 14th Croatian Biological Congress With International Participation; 2022 Oct 12-16; Pula, Croatia (2022):178-177,
https://hdl.handle.net/21.15107/rcub_ibiss_5619 .

TY  - JOUR
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Budečević, Sanja
AU  - Stojković, Biljana
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1111/een.13222
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5352
AB  - Expansion of the host range in phytophagous insects, followed by the specialisation on novel hosts, encompasses changes in many aspects of insects' behaviour, physiology, and the interaction between their life-history features. Here, we analyse the roles of insects' developmental plasticity in the process of host shift. Using laboratory populations of the seed beetle (Acanthoscelides obtectus), which have evolved on both optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years, we experimentally replicated the process of host shift and analysed the patterns of short-term and long-term life-history responses to host variation. In order to test whether selection for increased plasticity has an effect on host shifting processes, we used existing bean and chickpea adapted populations to establish new populations in which the host plant offered for insect development was changed each generation (for 13 generations). To test the potential for a short-term plastic response, beetles from each laboratory population were raised on both hosts for one generation. Results showed that, in contrast to the populations that evolved on beans, which maintained high levels of developmental plasticity, long-term host switching to chickpeas was accompanied with specialisation of pre-adult viability with a simultaneous increase in fecundity. Populations evolved on alternate plant hosts that revealed similar plasticity patterns as their ancestral populations. These results suggest that short-term plastic responses could determine the paths of long-term evolution of life-history plasticity. However, more time could be needed for plasticity to evolve differently from the initial responses.
T2  - Ecological Entomology
T1  - Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments
DO  - 10.1111/een.13222
ER  - 

@article{
author = "Savković, Uroš and Đorđević, Mirko and Vlajnić, Lea and Budečević, Sanja and Stojković, Biljana",
year = "2022",
abstract = "Expansion of the host range in phytophagous insects, followed by the specialisation on novel hosts, encompasses changes in many aspects of insects' behaviour, physiology, and the interaction between their life-history features. Here, we analyse the roles of insects' developmental plasticity in the process of host shift. Using laboratory populations of the seed beetle (Acanthoscelides obtectus), which have evolved on both optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years, we experimentally replicated the process of host shift and analysed the patterns of short-term and long-term life-history responses to host variation. In order to test whether selection for increased plasticity has an effect on host shifting processes, we used existing bean and chickpea adapted populations to establish new populations in which the host plant offered for insect development was changed each generation (for 13 generations). To test the potential for a short-term plastic response, beetles from each laboratory population were raised on both hosts for one generation. Results showed that, in contrast to the populations that evolved on beans, which maintained high levels of developmental plasticity, long-term host switching to chickpeas was accompanied with specialisation of pre-adult viability with a simultaneous increase in fecundity. Populations evolved on alternate plant hosts that revealed similar plasticity patterns as their ancestral populations. These results suggest that short-term plastic responses could determine the paths of long-term evolution of life-history plasticity. However, more time could be needed for plasticity to evolve differently from the initial responses.",
journal = "Ecological Entomology",
title = "Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments",
doi = "10.1111/een.13222"
}

Savković, U., Đorđević, M., Vlajnić, L., Budečević, S.,& Stojković, B.. (2022). Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments. in Ecological Entomology.
https://doi.org/10.1111/een.13222

Savković U, Đorđević M, Vlajnić L, Budečević S, Stojković B. Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments. in Ecological Entomology. 2022;.
doi:10.1111/een.13222 .

Savković, Uroš, Đorđević, Mirko, Vlajnić, Lea, Budečević, Sanja, Stojković, Biljana, "Evolution of developmental plasticity and the potential of host shift in the seed beetle: Insights from laboratory evolution experiments" in Ecological Entomology (2022),
https://doi.org/10.1111/een.13222 . .

TY  - CONF
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Budečević, Sanja
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5034
AB  - Eksperimentalna (laboratorijska) evolucija predstavlja savremen metodološki pristup u empirijskom testiranju evoluciono-bioloških hipoteza. Stavljajući model sisteme u uslove koji su unapred definisani i konstantni, eksperimentalnom evolucijom mogu se pratiti transgeneracijske fenotipske i genetičke promene populacija što omogućava proučavanje procesa adaptacija. Pojedine eksperimentalne populacije pasuljevog žiška su pod specifičnim režimima laboratorijske evolucije više od 35 godina i predstavljaju jedinstven primer višedecenijske eksperimentalne evolucije na svetu. Ovo predavanje obuhvatiće primere koji ilustruju evoluciju fenotipske plastičnosti osobina životne istorije u uslovima izmenjene biljke domaćina uključujući promene u ponašanju prilikom polaganja jaja, morfološke promene i populacionu dinamiku. Dodatano biće predstavljeni rezultati populacija koje su održavane u režimima za ranu i kasnu reprodukciju koje su dovele do velikih promena na molekularnom, biohemijskom i fiziološkom nivou. Konačno, posebna pažnja će biti posvećena primeni eksperimentalne evolucije u razvoju savremene metode kontrole brojnosti insekata koja se zasniva na specifičnim mitohondrijskim mutacijama s polno specifičnim efektima koji ograničavaju reproduktivni potencijal mužjaka bez negativnih efekata na ženke (tehnika trojanskih ženki).
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku
SP  - 93
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5034
ER  - 

@conference{
author = "Savković, Uroš and Đorđević, Mirko and Budečević, Sanja and Vlajnić, Lea and Stojković, Biljana",
year = "2022",
abstract = "Eksperimentalna (laboratorijska) evolucija predstavlja savremen metodološki pristup u empirijskom testiranju evoluciono-bioloških hipoteza. Stavljajući model sisteme u uslove koji su unapred definisani i konstantni, eksperimentalnom evolucijom mogu se pratiti transgeneracijske fenotipske i genetičke promene populacija što omogućava proučavanje procesa adaptacija. Pojedine eksperimentalne populacije pasuljevog žiška su pod specifičnim režimima laboratorijske evolucije više od 35 godina i predstavljaju jedinstven primer višedecenijske eksperimentalne evolucije na svetu. Ovo predavanje obuhvatiće primere koji ilustruju evoluciju fenotipske plastičnosti osobina životne istorije u uslovima izmenjene biljke domaćina uključujući promene u ponašanju prilikom polaganja jaja, morfološke promene i populacionu dinamiku. Dodatano biće predstavljeni rezultati populacija koje su održavane u režimima za ranu i kasnu reprodukciju koje su dovele do velikih promena na molekularnom, biohemijskom i fiziološkom nivou. Konačno, posebna pažnja će biti posvećena primeni eksperimentalne evolucije u razvoju savremene metode kontrole brojnosti insekata koja se zasniva na specifičnim mitohondrijskim mutacijama s polno specifičnim efektima koji ograničavaju reproduktivni potencijal mužjaka bez negativnih efekata na ženke (tehnika trojanskih ženki).",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku",
pages = "93",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5034"
}

Savković, U., Đorđević, M., Budečević, S., Vlajnić, L.,& Stojković, B.. (2022). Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 93.
https://hdl.handle.net/21.15107/rcub_ibiss_5034

Savković U, Đorđević M, Budečević S, Vlajnić L, Stojković B. Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:93.
https://hdl.handle.net/21.15107/rcub_ibiss_5034 .

Savković, Uroš, Đorđević, Mirko, Budečević, Sanja, Vlajnić, Lea, Stojković, Biljana, "Aktualne i višedecenijske studije eksperimentalne evolucije na pasuljevom žišku" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):93,
https://hdl.handle.net/21.15107/rcub_ibiss_5034 .

TY  - CONF
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Stojković, Biljana
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Đorđević, Mirko
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5031
AB  - Uniparental inheritance of mitochondria leads to asymmetry of mtDNA evolution because
selection acts directly on non-neutral mtDNA polymorphisms only through the female
lineage. In other words, natural selection will not recognize any mtDNA mutation which is
male harming, but neutral, beneficial, or slightly deleterious for females. Ultimately, non-
Mendelian inheritance of mitochondria drives the evolution of male-specific mitochondrial
mutation loads, an idea known as mother’s curse. Naturally occurring mtDNA mutations
that impair male fertility, but have no effects on females, have been recognized as an
opportunity for the development of the biocontrol of pest species termed Trojan Female
Technique (TFT). Namely, females carrying TFT mutations, and their female
descendants, could continuously, over multiple generations, produce males that sire
fewer offspring than wild-type counterparts. Although the TFT shows promise as a transgenerational,
self-sustaining method, its empirical success hinges on the existence of
natural TFT mutations that remain uncharacterized in pest insects. In order to identify
and test possible TFT candidates for biological control of seed beetle, a common pest in
legume storages, three mtDNA haplotypes found in laboratory lines were expressed
alongside one outbred nuclear background. We analyzed the effects of specific
mitotypes on female and male life history traits, and found a male-only reduction in
fertility in one mito-nuclear combination. Specifically, fertility (but not total fecundity and
lifespan) of males carrying specific mtDNA variant was reduced by 35% relative to the
controls. Our study provides evidence that intergenomic conflict leads to the existence of
male-harming mtDNA mutations that segregate within populations.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus
SP  - 204
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5031
ER  - 

@conference{
author = "Vlajnić, Lea and Savković, Uroš and Stojković, Biljana and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Đorđević, Mirko",
year = "2022",
abstract = "Uniparental inheritance of mitochondria leads to asymmetry of mtDNA evolution because
selection acts directly on non-neutral mtDNA polymorphisms only through the female
lineage. In other words, natural selection will not recognize any mtDNA mutation which is
male harming, but neutral, beneficial, or slightly deleterious for females. Ultimately, non-
Mendelian inheritance of mitochondria drives the evolution of male-specific mitochondrial
mutation loads, an idea known as mother’s curse. Naturally occurring mtDNA mutations
that impair male fertility, but have no effects on females, have been recognized as an
opportunity for the development of the biocontrol of pest species termed Trojan Female
Technique (TFT). Namely, females carrying TFT mutations, and their female
descendants, could continuously, over multiple generations, produce males that sire
fewer offspring than wild-type counterparts. Although the TFT shows promise as a transgenerational,
self-sustaining method, its empirical success hinges on the existence of
natural TFT mutations that remain uncharacterized in pest insects. In order to identify
and test possible TFT candidates for biological control of seed beetle, a common pest in
legume storages, three mtDNA haplotypes found in laboratory lines were expressed
alongside one outbred nuclear background. We analyzed the effects of specific
mitotypes on female and male life history traits, and found a male-only reduction in
fertility in one mito-nuclear combination. Specifically, fertility (but not total fecundity and
lifespan) of males carrying specific mtDNA variant was reduced by 35% relative to the
controls. Our study provides evidence that intergenomic conflict leads to the existence of
male-harming mtDNA mutations that segregate within populations.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus",
pages = "204",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5031"
}

Vlajnić, L., Savković, U., Stojković, B., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A.,& Đorđević, M.. (2022). Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 204.
https://hdl.handle.net/21.15107/rcub_ibiss_5031

Vlajnić L, Savković U, Stojković B, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Đorđević M. Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:204.
https://hdl.handle.net/21.15107/rcub_ibiss_5031 .

Vlajnić, Lea, Savković, Uroš, Stojković, Biljana, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Đorđević, Mirko, "Mitochondrial variant specifically impairs male fertility in seed beetle Acanthoscelides obtectus" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):204,
https://hdl.handle.net/21.15107/rcub_ibiss_5031 .

TY  - CONF
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Budečević, Sanja
AU  - Vlajnić, Lea
AU  - Pešić, Snežana
AU  - Vukajlović, Filip
AU  - Predojević, Dragana
AU  - Mitrovski Bogdanović, Ana
AU  - Stojković, Oliver
AU  - Stojković, Biljana
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5030
AB  - Diverse aspects of insects’ behaviour, physiology, and the relationship between life-history traits are challenged when insects try to expand their host range, exploit alternative food sources and specialise on them. Process that enables phytophagous insects to utilise new food sources, known as host shift, is tightly associated with developmental plasticity and is seldom studied in laboratory settings. Using an experimental evolution approach we simulated the host shift process and observed the evolution of plasticity in seed beetle (Acanthoscelides obtectus) laboratory populations that evolved on optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years. We have looked into: 1) life-history traits and how the long-term exposure to different hosts affects them; 2) the consequences when insects are exposed to short-term (in a single generation) change of the host plant, and 3) what happens when the host plant is altered each generation, that is, we observed  the process of the selection for increased plasticity in a laboratory setting. Prior to life-history assays, populations were in the experiment for 13 generations. We found that long-term host shift to chickpeas decreased plasticity levels for preadult traits compared to bean adapted populations. Simultaneously, fecundity evolved a more plastic response. Groups that were evolving in conditions where plant hosts were alternated each generation had the same plasticity patterns as their ancestral populations, suggesting the need for more time for plastic response to evolve. This research illustrates the importance of phenotypic plasticity in maintaining populations under changing feeding conditions.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
T1  - Developmental plasticity and the potential of host shift in the seed beetle
SP  - 184
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5030
ER  - 

@conference{
author = "Savković, Uroš and Đorđević, Mirko and Budečević, Sanja and Vlajnić, Lea and Pešić, Snežana and Vukajlović, Filip and Predojević, Dragana and Mitrovski Bogdanović, Ana and Stojković, Oliver and Stojković, Biljana",
year = "2022",
abstract = "Diverse aspects of insects’ behaviour, physiology, and the relationship between life-history traits are challenged when insects try to expand their host range, exploit alternative food sources and specialise on them. Process that enables phytophagous insects to utilise new food sources, known as host shift, is tightly associated with developmental plasticity and is seldom studied in laboratory settings. Using an experimental evolution approach we simulated the host shift process and observed the evolution of plasticity in seed beetle (Acanthoscelides obtectus) laboratory populations that evolved on optimal (common beans) and suboptimal (chickpea) plant hosts for more than 35 years. We have looked into: 1) life-history traits and how the long-term exposure to different hosts affects them; 2) the consequences when insects are exposed to short-term (in a single generation) change of the host plant, and 3) what happens when the host plant is altered each generation, that is, we observed  the process of the selection for increased plasticity in a laboratory setting. Prior to life-history assays, populations were in the experiment for 13 generations. We found that long-term host shift to chickpeas decreased plasticity levels for preadult traits compared to bean adapted populations. Simultaneously, fecundity evolved a more plastic response. Groups that were evolving in conditions where plant hosts were alternated each generation had the same plasticity patterns as their ancestral populations, suggesting the need for more time for plastic response to evolve. This research illustrates the importance of phenotypic plasticity in maintaining populations under changing feeding conditions.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic",
title = "Developmental plasticity and the potential of host shift in the seed beetle",
pages = "184",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5030"
}

Savković, U., Đorđević, M., Budečević, S., Vlajnić, L., Pešić, S., Vukajlović, F., Predojević, D., Mitrovski Bogdanović, A., Stojković, O.,& Stojković, B.. (2022). Developmental plasticity and the potential of host shift in the seed beetle. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic
European Society for Evolutionary Biology., 184.
https://hdl.handle.net/21.15107/rcub_ibiss_5030

Savković U, Đorđević M, Budečević S, Vlajnić L, Pešić S, Vukajlović F, Predojević D, Mitrovski Bogdanović A, Stojković O, Stojković B. Developmental plasticity and the potential of host shift in the seed beetle. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic. 2022;:184.
https://hdl.handle.net/21.15107/rcub_ibiss_5030 .

Savković, Uroš, Đorđević, Mirko, Budečević, Sanja, Vlajnić, Lea, Pešić, Snežana, Vukajlović, Filip, Predojević, Dragana, Mitrovski Bogdanović, Ana, Stojković, Oliver, Stojković, Biljana, "Developmental plasticity and the potential of host shift in the seed beetle" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Pague, Czech Republic (2022):184,
https://hdl.handle.net/21.15107/rcub_ibiss_5030 .

TY  - JOUR
AU  - Budečević, Sanja
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Vlajnić, Lea
AU  - Stojković, Biljana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4201
AB  - Sexual dimorphism and specific patterns of development contribute in a great manner to the direction and degree of the sexual differences in body size and shape in many insects. Using a landmark-based geometric morpohometrics approach, we investigated sex-specific morphological size and shape variation in the seed beetle, Acanthoscelides obtectus. We also tested the functional hypothesis of the two morphological modules—thorax and abdomen in both sexes. Female-biased sexual dimorphism in size was shown, while differences in shape were reflected in the wider thorax and abdomen and shorter abdomen in females in comparison to males. The functional hypothesis of a two-module body was confirmed only in females before correction for size, and in both sexes after the allometry correction. Our results indicate that reproductive function has the central role in forming the patterns of modularity. We hypothesize that high morphological integration of the abdomen in females results from intense stabilizing selection, while the more relaxed integration in males is driven by the higher intensity of sexual selection.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Insects
T1  - Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach
IS  - 4
VL  - 12
DO  - 10.3390/insects12040350
SP  - 350
ER  - 

@article{
author = "Budečević, Sanja and Savković, Uroš and Đorđević, Mirko and Vlajnić, Lea and Stojković, Biljana",
year = "2021",
abstract = "Sexual dimorphism and specific patterns of development contribute in a great manner to the direction and degree of the sexual differences in body size and shape in many insects. Using a landmark-based geometric morpohometrics approach, we investigated sex-specific morphological size and shape variation in the seed beetle, Acanthoscelides obtectus. We also tested the functional hypothesis of the two morphological modules—thorax and abdomen in both sexes. Female-biased sexual dimorphism in size was shown, while differences in shape were reflected in the wider thorax and abdomen and shorter abdomen in females in comparison to males. The functional hypothesis of a two-module body was confirmed only in females before correction for size, and in both sexes after the allometry correction. Our results indicate that reproductive function has the central role in forming the patterns of modularity. We hypothesize that high morphological integration of the abdomen in females results from intense stabilizing selection, while the more relaxed integration in males is driven by the higher intensity of sexual selection.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Insects",
title = "Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach",
number = "4",
volume = "12",
doi = "10.3390/insects12040350",
pages = "350"
}

Budečević, S., Savković, U., Đorđević, M., Vlajnić, L.,& Stojković, B.. (2021). Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach. in Insects
Multidisciplinary Digital Publishing Institute., 12(4), 350.
https://doi.org/10.3390/insects12040350

Budečević S, Savković U, Đorđević M, Vlajnić L, Stojković B. Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach. in Insects. 2021;12(4):350.
doi:10.3390/insects12040350 .

Budečević, Sanja, Savković, Uroš, Đorđević, Mirko, Vlajnić, Lea, Stojković, Biljana, "Sexual Dimorphism and Morphological Modularity in Acanthoscelides obtectus (Say, 1831) (Coleoptera: Chrysomelidae): A Geometric Morphometric Approach" in Insects, 12, no. 4 (2021):350,
https://doi.org/10.3390/insects12040350 . .

TY  - JOUR
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
PY  - 2019
UR  - https://www.mdpi.com/2075-4450/10/6/153
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3357
AB  - Effective pest management strategies for a targeted pest species must rely on accurate, reliable and reproducible estimates of population dynamics. Importance of such approaches is even more conspicuous when assessing pest's potential to utilize other stored products. Using an experimental evolution approach, we have focused our attention on a common bean pest, the seed beetle (Acanthoscelides obtectus). We looked into the potential to invade and sustain population growth on two suboptimal host plants (chickpeas and mung beans). Such an approach simulates steps of the host-shift process in storages. By analyzing population dynamics during initial encountering with a new host plant, we detected a population drop for both novel hosts. However, transgenerational development in a novel environment resulted in a constant population growth in chickpeas, but not in mung bean populations. Reversal of chickpea selected populations to original host plant has led to a severe decrease in population parameters due to low viability of immatures, while the opposite trend was detected in mung bean populations. This paper highlights the importance of good practice in estimating population dynamics for economically important species. With special emphasis on storage pest species, we discuss how this approach can be useful for estimating invading potential of pest insects.
T2  - Insects
T1  - Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments.
IS  - 6
VL  - 10
DO  - 10.3390/insects10060153
SP  - 153
ER  - 

@article{
author = "Savković, Uroš and Đorđević, Mirko and Stojković, Biljana",
year = "2019",
abstract = "Effective pest management strategies for a targeted pest species must rely on accurate, reliable and reproducible estimates of population dynamics. Importance of such approaches is even more conspicuous when assessing pest's potential to utilize other stored products. Using an experimental evolution approach, we have focused our attention on a common bean pest, the seed beetle (Acanthoscelides obtectus). We looked into the potential to invade and sustain population growth on two suboptimal host plants (chickpeas and mung beans). Such an approach simulates steps of the host-shift process in storages. By analyzing population dynamics during initial encountering with a new host plant, we detected a population drop for both novel hosts. However, transgenerational development in a novel environment resulted in a constant population growth in chickpeas, but not in mung bean populations. Reversal of chickpea selected populations to original host plant has led to a severe decrease in population parameters due to low viability of immatures, while the opposite trend was detected in mung bean populations. This paper highlights the importance of good practice in estimating population dynamics for economically important species. With special emphasis on storage pest species, we discuss how this approach can be useful for estimating invading potential of pest insects.",
journal = "Insects",
title = "Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments.",
number = "6",
volume = "10",
doi = "10.3390/insects10060153",
pages = "153"
}

Savković, U., Đorđević, M.,& Stojković, B.. (2019). Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments.. in Insects, 10(6), 153.
https://doi.org/10.3390/insects10060153

Savković U, Đorđević M, Stojković B. Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments.. in Insects. 2019;10(6):153.
doi:10.3390/insects10060153 .

Savković, Uroš, Đorđević, Mirko, Stojković, Biljana, "Potential for Acanthoscelides obtectus to Adapt to New Hosts Seen in Laboratory Selection Experiments." in Insects, 10, no. 6 (2019):153,
https://doi.org/10.3390/insects10060153 . .

TY  - CONF
AU  - Vlajnić, Lea
AU  - Savković, Uroš
AU  - Stojković, Biljana
AU  - Đorđević, Mirko
PY  - 2019
UR  - https://app.oxfordabstracts.com/events/653/program-app/submission/122870
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3456
AB  - Pest species pose one of the biggest threats to global agriculture, economy and human health. Thus, there is ongoing effort to implement pest control techniques that are often costly and detrimental for environment and non-target species. The Trojan Female Technique (TFT) is a novel approach to pest management via control of reproductive output. The goal of the TFT is to use naturally occurring mtDNA mutations which impair male fertility, but have no effects on females, to achieve sustained pest population suppression. Although described in fruit flies, the candidate TFT mutations have not yet been documented in pest species. In order to identify and test possible TFT candidates for biological control of seed beetle Acanthoscelides obtectus, a common pest in legume storages, we expressed four mtDNA haplotypes found in laboratory lines selected for late reproduction/long life alongside nuclear background of wild populations. Analysis of the effect of specific mitotypes on female and male reproductive potential showed significant male-only reduction in fertility in just one mito-nuclear combination. More specifically, the fertility of males carrying MG3a haplotype was reduced for more than 40% relative to MG3a females. This result indicates MG3a as a promising candidate for further development of the TFT in seed beetles.
C3  - 17th Congress of the  European Society for Evolutionary Biology
T1  - Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus
SP  - S16.P5
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3456
ER  - 

@conference{
author = "Vlajnić, Lea and Savković, Uroš and Stojković, Biljana and Đorđević, Mirko",
year = "2019",
abstract = "Pest species pose one of the biggest threats to global agriculture, economy and human health. Thus, there is ongoing effort to implement pest control techniques that are often costly and detrimental for environment and non-target species. The Trojan Female Technique (TFT) is a novel approach to pest management via control of reproductive output. The goal of the TFT is to use naturally occurring mtDNA mutations which impair male fertility, but have no effects on females, to achieve sustained pest population suppression. Although described in fruit flies, the candidate TFT mutations have not yet been documented in pest species. In order to identify and test possible TFT candidates for biological control of seed beetle Acanthoscelides obtectus, a common pest in legume storages, we expressed four mtDNA haplotypes found in laboratory lines selected for late reproduction/long life alongside nuclear background of wild populations. Analysis of the effect of specific mitotypes on female and male reproductive potential showed significant male-only reduction in fertility in just one mito-nuclear combination. More specifically, the fertility of males carrying MG3a haplotype was reduced for more than 40% relative to MG3a females. This result indicates MG3a as a promising candidate for further development of the TFT in seed beetles.",
journal = "17th Congress of the  European Society for Evolutionary Biology",
title = "Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus",
pages = "S16.P5",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3456"
}

Vlajnić, L., Savković, U., Stojković, B.,& Đorđević, M.. (2019). Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus. in 17th Congress of the  European Society for Evolutionary Biology, S16.P5.
https://hdl.handle.net/21.15107/rcub_ibiss_3456

Vlajnić L, Savković U, Stojković B, Đorđević M. Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus. in 17th Congress of the  European Society for Evolutionary Biology. 2019;:S16.P5.
https://hdl.handle.net/21.15107/rcub_ibiss_3456 .

Vlajnić, Lea, Savković, Uroš, Stojković, Biljana, Đorđević, Mirko, "Sex-specific effects of candidate Trojan Female Technique haplotype on fertility in pest species Acanthoscelides obtectus" in 17th Congress of the  European Society for Evolutionary Biology (2019):S16.P5,
https://hdl.handle.net/21.15107/rcub_ibiss_3456 .

TY  - JOUR
AU  - Nešić, Milica J.
AU  - Stojković, Biljana
AU  - Marić, Nađa P.
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/pcn.12933
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3514
AB  - Considering the relatively high heritability of schizophrenia and the fact that it significantly reduces the reproductive fitness of affected individuals, it is not clear how the disorder is still maintained in human populations at a disproportionally high prevalence. Many theories propose that the disorder is a result of a trade-off between costs and benefits of the evolution of exclusively human adaptations. There have also been suggestions that schizophrenia risk alleles are accompanied with increase in fitness of affected persons or their relatives in both past and current social contexts. The discoveries of novel schizophrenia-related genes and the advancements in comparative genomics (especially comparisons of the human genome and the genomes of related species, such as chimpanzees and extinct hominids) have finally made certain evolutionary theories testable. In this paper, we review the current understanding of the genetics of schizophrenia, the basic principles of evolution that complement our understanding of the subject, and the latest genetic studies that examine long-standing evolutionary theories of schizophrenia using novel methodologies and data. We find that the origin of schizophrenia is complex and likely governed by different evolutionary mechanisms that are not mutually exclusive. Furthermore, the most recent evidence implies that schizophrenia cannot be comprehended as a trait that has elevated fitness in human evolutionary lineage, but has been a mildly deleterious by-product of specific patterns of the evolution of the human brain. In other words, novel findings do not support previous hypotheses stating that schizophrenia risk genes have an evolutionary advantage.
T2  - Psychiatry and Clinical Neurosciences
T1  - On the origin of schizophrenia: Testing evolutionary theories in the post-genomic era.
DO  - 10.1111/pcn.12933
ER  - 

@article{
author = "Nešić, Milica J. and Stojković, Biljana and Marić, Nađa P.",
year = "2019",
abstract = "Considering the relatively high heritability of schizophrenia and the fact that it significantly reduces the reproductive fitness of affected individuals, it is not clear how the disorder is still maintained in human populations at a disproportionally high prevalence. Many theories propose that the disorder is a result of a trade-off between costs and benefits of the evolution of exclusively human adaptations. There have also been suggestions that schizophrenia risk alleles are accompanied with increase in fitness of affected persons or their relatives in both past and current social contexts. The discoveries of novel schizophrenia-related genes and the advancements in comparative genomics (especially comparisons of the human genome and the genomes of related species, such as chimpanzees and extinct hominids) have finally made certain evolutionary theories testable. In this paper, we review the current understanding of the genetics of schizophrenia, the basic principles of evolution that complement our understanding of the subject, and the latest genetic studies that examine long-standing evolutionary theories of schizophrenia using novel methodologies and data. We find that the origin of schizophrenia is complex and likely governed by different evolutionary mechanisms that are not mutually exclusive. Furthermore, the most recent evidence implies that schizophrenia cannot be comprehended as a trait that has elevated fitness in human evolutionary lineage, but has been a mildly deleterious by-product of specific patterns of the evolution of the human brain. In other words, novel findings do not support previous hypotheses stating that schizophrenia risk genes have an evolutionary advantage.",
journal = "Psychiatry and Clinical Neurosciences",
title = "On the origin of schizophrenia: Testing evolutionary theories in the post-genomic era.",
doi = "10.1111/pcn.12933"
}

Nešić, M. J., Stojković, B.,& Marić, N. P.. (2019). On the origin of schizophrenia: Testing evolutionary theories in the post-genomic era.. in Psychiatry and Clinical Neurosciences.
https://doi.org/10.1111/pcn.12933

Nešić MJ, Stojković B, Marić NP. On the origin of schizophrenia: Testing evolutionary theories in the post-genomic era.. in Psychiatry and Clinical Neurosciences. 2019;.
doi:10.1111/pcn.12933 .

Nešić, Milica J., Stojković, Biljana, Marić, Nađa P., "On the origin of schizophrenia: Testing evolutionary theories in the post-genomic era." in Psychiatry and Clinical Neurosciences (2019),
https://doi.org/10.1111/pcn.12933 . .

TY  - JOUR
AU  - Martinossi-Allibert, Ivain
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Arnqvist, Göran
AU  - Stojković, Biljana
AU  - Berger, David
PY  - 2018
UR  - http://doi.wiley.com/10.1111/evo.13412
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29238970
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2963
AB  - Whether sexual selection generally promotes or impedes population persistence remains an open question. Intralocus sexual conflict (IaSC) can render sexual selection in males detrimental to the population by increasing the frequency of alleles with positive effects on male reproductive success but negative effects on female fecundity. Recent modeling based on fitness landscape theory, however, indicates that the relative impact of IaSC may be reduced in maladapted populations and that sexual selection therefore might promote adaptation when it is most needed. Here, we test this prediction using bean beetles that had undergone 80 generations of experimental evolution on two alternative host plants. We isolated and assessed the effect of maladaptation on sex-specific strengths of selection and IaSC by cross-rearing the two experimental evolution regimes on the alternative hosts and estimating within-population genetic (co)variance for fitness in males and females. Two key predictions were upheld: males generally experienced stronger selection compared to females and maladaptation increased selection in females. However, maladaptation consistently decreased male-bias in the strength of selection and IaSC was not reduced in maladapted populations. These findings imply that sexual selection can be disrupted in stressful environmental conditions, thus reducing one of the potential benefits of sexual reproduction in maladapted populations.
T2  - Evolution
T1  - The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.
DO  - 10.1111/evo.13412
ER  - 

@article{
author = "Martinossi-Allibert, Ivain and Savković, Uroš and Đorđević, Mirko and Arnqvist, Göran and Stojković, Biljana and Berger, David",
year = "2018",
abstract = "Whether sexual selection generally promotes or impedes population persistence remains an open question. Intralocus sexual conflict (IaSC) can render sexual selection in males detrimental to the population by increasing the frequency of alleles with positive effects on male reproductive success but negative effects on female fecundity. Recent modeling based on fitness landscape theory, however, indicates that the relative impact of IaSC may be reduced in maladapted populations and that sexual selection therefore might promote adaptation when it is most needed. Here, we test this prediction using bean beetles that had undergone 80 generations of experimental evolution on two alternative host plants. We isolated and assessed the effect of maladaptation on sex-specific strengths of selection and IaSC by cross-rearing the two experimental evolution regimes on the alternative hosts and estimating within-population genetic (co)variance for fitness in males and females. Two key predictions were upheld: males generally experienced stronger selection compared to females and maladaptation increased selection in females. However, maladaptation consistently decreased male-bias in the strength of selection and IaSC was not reduced in maladapted populations. These findings imply that sexual selection can be disrupted in stressful environmental conditions, thus reducing one of the potential benefits of sexual reproduction in maladapted populations.",
journal = "Evolution",
title = "The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.",
doi = "10.1111/evo.13412"
}

Martinossi-Allibert, I., Savković, U., Đorđević, M., Arnqvist, G., Stojković, B.,& Berger, D.. (2018). The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.. in Evolution.
https://doi.org/10.1111/evo.13412

Martinossi-Allibert I, Savković U, Đorđević M, Arnqvist G, Stojković B, Berger D. The consequences of sexual selection in well-adapted and maladapted populations of bean beetles.. in Evolution. 2018;.
doi:10.1111/evo.13412 .

Martinossi-Allibert, Ivain, Savković, Uroš, Đorđević, Mirko, Arnqvist, Göran, Stojković, Biljana, Berger, David, "The consequences of sexual selection in well-adapted and maladapted populations of bean beetles." in Evolution (2018),
https://doi.org/10.1111/evo.13412 . .

TY  - JOUR
AU  - Arnqvist, Göran
AU  - Stojković, Biljana
AU  - Rönn, Johanna L.
AU  - Immonen, Elina
AU  - White, Craig
PY  - 2017
UR  - http://doi.wiley.com/10.1111/1365-2435.12927
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2820
AB  - Metabolic rate (MR) is a key functional trait simply because metabolism converts resources into population growth rate. Yet, our empirical understanding of the sources of within species variation in MR, as well as of its life history and ecological correlates, is rather limited. Here, we assess whether MR lies at the root of a syndrome of correlated rate-dependent life-history traits in an insect. Selection for early (E) or late (L) age-at-reproduction for > 160 generations in the bean beetle Acanthoscelides obtectus has produced beetles that differ markedly in juvenile development, body size, fecundity schedules, ageing and life span. Here, we use micro-respirometry to test whether this has been associated with the evolution of age- and sex-specific metabolic phenotypes. We find that mass-specific MR is 18% higher in E lines compared to L lines and that MR decreases more rapidly with chronological, but not biological, age in E lines. Males, under sexual selection to "live-fast-die-young", show 50% higher MR than females and MR decreased more rapidly with age in males. Our results are consistent with a central role for MR for the divergence in "pace-of-life" seen in these beetles, supporting the view that MR lies at the root of ecologically relevant life-history trait variation within species.
T2  - Functional Ecology
T1  - The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles
DO  - 10.1111/1365-2435.12927
ER  - 

@article{
author = "Arnqvist, Göran and Stojković, Biljana and Rönn, Johanna L. and Immonen, Elina and White, Craig",
year = "2017",
abstract = "Metabolic rate (MR) is a key functional trait simply because metabolism converts resources into population growth rate. Yet, our empirical understanding of the sources of within species variation in MR, as well as of its life history and ecological correlates, is rather limited. Here, we assess whether MR lies at the root of a syndrome of correlated rate-dependent life-history traits in an insect. Selection for early (E) or late (L) age-at-reproduction for > 160 generations in the bean beetle Acanthoscelides obtectus has produced beetles that differ markedly in juvenile development, body size, fecundity schedules, ageing and life span. Here, we use micro-respirometry to test whether this has been associated with the evolution of age- and sex-specific metabolic phenotypes. We find that mass-specific MR is 18% higher in E lines compared to L lines and that MR decreases more rapidly with chronological, but not biological, age in E lines. Males, under sexual selection to "live-fast-die-young", show 50% higher MR than females and MR decreased more rapidly with age in males. Our results are consistent with a central role for MR for the divergence in "pace-of-life" seen in these beetles, supporting the view that MR lies at the root of ecologically relevant life-history trait variation within species.",
journal = "Functional Ecology",
title = "The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles",
doi = "10.1111/1365-2435.12927"
}

Arnqvist, G., Stojković, B., Rönn, J. L., Immonen, E.,& White, C.. (2017). The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles. in Functional Ecology.
https://doi.org/10.1111/1365-2435.12927

Arnqvist G, Stojković B, Rönn JL, Immonen E, White C. The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles. in Functional Ecology. 2017;.
doi:10.1111/1365-2435.12927 .

Arnqvist, Göran, Stojković, Biljana, Rönn, Johanna L., Immonen, Elina, White, Craig, "The pace-of-life: A sex-specific link between metabolic rate and life history in bean beetles" in Functional Ecology (2017),
https://doi.org/10.1111/1365-2435.12927 . .

TY  - JOUR
AU  - Stojković, Biljana
AU  - Sayadi, Ahmed
AU  - Đorđević, Mirko
AU  - Jović, Jelena
AU  - Savković, Uroš
AU  - Arnqvist, Göran
PY  - 2017
UR  - http://doi.wiley.com/10.1111/evo.13102
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2550
AB  - Mitochondria play a key role in ageing. The pursuit of genes that regulate variation in life span and ageing have shown that several nuclear-encoded mitochondrial genes are important. However, the role of mitochondrial encoded genes (mtDNA) is more controversial and our appreciation of the role of mtDNA for the evolution of life span is limited. We use replicated lines of seed beetles that have been artificially selected for long or short life for >190 generations, now showing dramatic phenotypic differences, to test for a possible role of mtDNA in the divergent evolution of ageing and life span. We show that these divergent selection regimes led to the evolution of significantly different mtDNA haplotype frequencies. Selection for a long life and late reproduction generated positive selection for one specific haplotype, which was fixed in most such lines. In contrast, selection for reproduction early in life led to both positive selection as well as negative frequency-dependent selection on two different haplotypes, which were both present in all such lines. Our findings suggest that the evolution of life span was in part mediated by mtDNA, providing support for the emerging general tenet that adaptive evolution of life-history syndromes may involve mtDNA.
T2  - Evolution
T1  - Divergent evolution of life span associated with mitochondrial DNA evolution
IS  - 1
VL  - 71
DO  - 10.1111/evo.13102
SP  - 160
EP  - 166
ER  - 

@article{
author = "Stojković, Biljana and Sayadi, Ahmed and Đorđević, Mirko and Jović, Jelena and Savković, Uroš and Arnqvist, Göran",
year = "2017",
abstract = "Mitochondria play a key role in ageing. The pursuit of genes that regulate variation in life span and ageing have shown that several nuclear-encoded mitochondrial genes are important. However, the role of mitochondrial encoded genes (mtDNA) is more controversial and our appreciation of the role of mtDNA for the evolution of life span is limited. We use replicated lines of seed beetles that have been artificially selected for long or short life for >190 generations, now showing dramatic phenotypic differences, to test for a possible role of mtDNA in the divergent evolution of ageing and life span. We show that these divergent selection regimes led to the evolution of significantly different mtDNA haplotype frequencies. Selection for a long life and late reproduction generated positive selection for one specific haplotype, which was fixed in most such lines. In contrast, selection for reproduction early in life led to both positive selection as well as negative frequency-dependent selection on two different haplotypes, which were both present in all such lines. Our findings suggest that the evolution of life span was in part mediated by mtDNA, providing support for the emerging general tenet that adaptive evolution of life-history syndromes may involve mtDNA.",
journal = "Evolution",
title = "Divergent evolution of life span associated with mitochondrial DNA evolution",
number = "1",
volume = "71",
doi = "10.1111/evo.13102",
pages = "160-166"
}

Stojković, B., Sayadi, A., Đorđević, M., Jović, J., Savković, U.,& Arnqvist, G.. (2017). Divergent evolution of life span associated with mitochondrial DNA evolution. in Evolution, 71(1), 160-166.
https://doi.org/10.1111/evo.13102

Stojković B, Sayadi A, Đorđević M, Jović J, Savković U, Arnqvist G. Divergent evolution of life span associated with mitochondrial DNA evolution. in Evolution. 2017;71(1):160-166.
doi:10.1111/evo.13102 .

Stojković, Biljana, Sayadi, Ahmed, Đorđević, Mirko, Jović, Jelena, Savković, Uroš, Arnqvist, Göran, "Divergent evolution of life span associated with mitochondrial DNA evolution" in Evolution, 71, no. 1 (2017):160-166,
https://doi.org/10.1111/evo.13102 . .

TY  - JOUR
AU  - Lazarević, Jelica
AU  - Janković Tomanić, Milena
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Milanović, Slobodan
AU  - Stojković, Biljana
PY  - 2017
UR  - http://doi.wiley.com/10.1111/ens.12250
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2924
AB  - The gypsy moth is a generalist insect pest with an extremely wide host range. Adaptive responses of digestive enzymes are important for the successful utilization of plant hosts that differ in the contents and ratios of constituent nutrients and allelochemicals. In the present study, we examined the responses of α-amylase, trypsin, and leucine aminopeptidase to two tree hosts (suitable oak, Quercus cerris, and unsuitable locust tree, Robinia pseudoacacia) in the fourth, fifth, and sixth instars of gypsy moth larvae originating from oak and locust tree forest populations (hereafter assigned as Quercus and Robinia populations, respectively). Gypsy moths from the Robinia forest had been adapting to this unsuitable host for more than 40 generations. To test for population-level host plant specialization, we applied a two-population × two-host experimental design. We compared the levels, developmental patterns, and plasticities of the activities of enzymes. The locust tree diet increased enzyme activity in the fourth instar and reduced activity in advanced instars of the Quercus larvae in comparison to the oak diet. These larvae also exhibited opposite developmental trajectories on the two hosts, i.e. activity increased on the oak diet and decreased on the locust tree diet with the progress of instar. Larvae of the Robinia population were characterized by reduced plasticity of enzyme activity and its developmental trajectories. In addition, elevated trypsin activity in response to an unsuitable host was observed in all instar larvae of the Robinia population, which demonstrated that Robinia larvae had an improved digestive performance than did Quercus larvae.
T2  - Entomological Science
T1  - Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)
IS  - 1
VL  - 20
DO  - 10.1111/ens.12250
SP  - 189
EP  - 194
ER  - 

@article{
author = "Lazarević, Jelica and Janković Tomanić, Milena and Savković, Uroš and Đorđević, Mirko and Milanović, Slobodan and Stojković, Biljana",
year = "2017",
abstract = "The gypsy moth is a generalist insect pest with an extremely wide host range. Adaptive responses of digestive enzymes are important for the successful utilization of plant hosts that differ in the contents and ratios of constituent nutrients and allelochemicals. In the present study, we examined the responses of α-amylase, trypsin, and leucine aminopeptidase to two tree hosts (suitable oak, Quercus cerris, and unsuitable locust tree, Robinia pseudoacacia) in the fourth, fifth, and sixth instars of gypsy moth larvae originating from oak and locust tree forest populations (hereafter assigned as Quercus and Robinia populations, respectively). Gypsy moths from the Robinia forest had been adapting to this unsuitable host for more than 40 generations. To test for population-level host plant specialization, we applied a two-population × two-host experimental design. We compared the levels, developmental patterns, and plasticities of the activities of enzymes. The locust tree diet increased enzyme activity in the fourth instar and reduced activity in advanced instars of the Quercus larvae in comparison to the oak diet. These larvae also exhibited opposite developmental trajectories on the two hosts, i.e. activity increased on the oak diet and decreased on the locust tree diet with the progress of instar. Larvae of the Robinia population were characterized by reduced plasticity of enzyme activity and its developmental trajectories. In addition, elevated trypsin activity in response to an unsuitable host was observed in all instar larvae of the Robinia population, which demonstrated that Robinia larvae had an improved digestive performance than did Quercus larvae.",
journal = "Entomological Science",
title = "Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)",
number = "1",
volume = "20",
doi = "10.1111/ens.12250",
pages = "189-194"
}

Lazarević, J., Janković Tomanić, M., Savković, U., Đorđević, M., Milanović, S.,& Stojković, B.. (2017). Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae). in Entomological Science, 20(1), 189-194.
https://doi.org/10.1111/ens.12250

Lazarević J, Janković Tomanić M, Savković U, Đorđević M, Milanović S, Stojković B. Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae). in Entomological Science. 2017;20(1):189-194.
doi:10.1111/ens.12250 .

Lazarević, Jelica, Janković Tomanić, Milena, Savković, Uroš, Đorđević, Mirko, Milanović, Slobodan, Stojković, Biljana, "Host-associated divergence in the activity of digestive enzymes in two populations of the gypsy moth Lymantria dispar (Lepidoptera: Erebidae)" in Entomological Science, 20, no. 1 (2017):189-194,
https://doi.org/10.1111/ens.12250 . .

TY  - JOUR
AU  - Stojković, Biljana
AU  - Đorđević, Mirko
PY  - 2017
UR  - https://ojs.pmf.uns.ac.rs/index.php/dbe_serbica/article/view/6493
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2816
AB  - The evolution of eukaryotes is based on dynamic coevolutionary interactions between the two genomes, nuclear (nDNA) and mitochondrial (mtDNA). Current evidence suggests that the origin of eukaryotes corresponds to the origin of mitochondria. The primary center of adenosine triphosphate (ATP) production through the process of oxidative phosphorylation (OXPHOS), which is based on the functioning of four large protein complexes that are responsible for the proton gradient across the inner mitochondrial membrane. These complexes in the electron transport chain (ETC) are composed of polypeptides encoded by both mitochondrial and nuclear genes. In order to preserve the uncompromised functionality of mitochondria, i.e. the adequate coupling of all interacting subunits in OXPHOS, the two genomes had to coevolve. In other words, mitonuclear compatibilities are required for optimal life-history of an organism because even minor biochemical inefficiency can have major fitness consequences by modulating energetic efficiency and oxidative stress levels. The link between life-history evolution and mitonuclear interactions is deeply rooted within the mechanisms of energy metabolism. The coevolved epistatic interactions between mitochondria and nucleus determine the amount of energy available for all biological functions. Selective optimization of one life-history function (e.g. reproduction) may come at the cost of reduced competence for somatic maintenance, viability and survival due to mutually exclusive energy allocation to distinct functions. Different approaches in investigating the central roles of mitochondrial metabolic processes and mitonuclear epistasis in life-history evolution are discussed in this paper.
T2  - Biologia Serbica
T1  - Interaction between mitochondrial and nuclear genomes: the role in life-history evolution
IS  - 1
VL  - 39
DO  - 10.5281/zenodo.826619
SP  - 32
EP  - 40
ER  - 

@article{
author = "Stojković, Biljana and Đorđević, Mirko",
year = "2017",
abstract = "The evolution of eukaryotes is based on dynamic coevolutionary interactions between the two genomes, nuclear (nDNA) and mitochondrial (mtDNA). Current evidence suggests that the origin of eukaryotes corresponds to the origin of mitochondria. The primary center of adenosine triphosphate (ATP) production through the process of oxidative phosphorylation (OXPHOS), which is based on the functioning of four large protein complexes that are responsible for the proton gradient across the inner mitochondrial membrane. These complexes in the electron transport chain (ETC) are composed of polypeptides encoded by both mitochondrial and nuclear genes. In order to preserve the uncompromised functionality of mitochondria, i.e. the adequate coupling of all interacting subunits in OXPHOS, the two genomes had to coevolve. In other words, mitonuclear compatibilities are required for optimal life-history of an organism because even minor biochemical inefficiency can have major fitness consequences by modulating energetic efficiency and oxidative stress levels. The link between life-history evolution and mitonuclear interactions is deeply rooted within the mechanisms of energy metabolism. The coevolved epistatic interactions between mitochondria and nucleus determine the amount of energy available for all biological functions. Selective optimization of one life-history function (e.g. reproduction) may come at the cost of reduced competence for somatic maintenance, viability and survival due to mutually exclusive energy allocation to distinct functions. Different approaches in investigating the central roles of mitochondrial metabolic processes and mitonuclear epistasis in life-history evolution are discussed in this paper.",
journal = "Biologia Serbica",
title = "Interaction between mitochondrial and nuclear genomes: the role in life-history evolution",
number = "1",
volume = "39",
doi = "10.5281/zenodo.826619",
pages = "32-40"
}

Stojković, B.,& Đorđević, M.. (2017). Interaction between mitochondrial and nuclear genomes: the role in life-history evolution. in Biologia Serbica, 39(1), 32-40.
https://doi.org/10.5281/zenodo.826619

Stojković B, Đorđević M. Interaction between mitochondrial and nuclear genomes: the role in life-history evolution. in Biologia Serbica. 2017;39(1):32-40.
doi:10.5281/zenodo.826619 .

Stojković, Biljana, Đorđević, Mirko, "Interaction between mitochondrial and nuclear genomes: the role in life-history evolution" in Biologia Serbica, 39, no. 1 (2017):32-40,
https://doi.org/10.5281/zenodo.826619 . .

TY  - JOUR
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Savković, Uroš
AU  - Immonen, Elina
AU  - Tucić, Nikola
AU  - Lazarević, Jelica
AU  - Arnqvist, Göran
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2600
AB  - The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.
T2  - Evolution
T1  - Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles
IS  - 2
VL  - 71
DO  - 10.1111/evo.13109
SP  - 274
EP  - 288
ER  - 

@article{
author = "Đorđević, Mirko and Stojković, Biljana and Savković, Uroš and Immonen, Elina and Tucić, Nikola and Lazarević, Jelica and Arnqvist, Göran",
year = "2017",
abstract = "The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.",
journal = "Evolution",
title = "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles",
number = "2",
volume = "71",
doi = "10.1111/evo.13109",
pages = "274-288"
}

Đorđević, M., Stojković, B., Savković, U., Immonen, E., Tucić, N., Lazarević, J.,& Arnqvist, G.. (2017). Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution, 71(2), 274-288.
https://doi.org/10.1111/evo.13109

Đorđević M, Stojković B, Savković U, Immonen E, Tucić N, Lazarević J, Arnqvist G. Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution. 2017;71(2):274-288.
doi:10.1111/evo.13109 .

Đorđević, Mirko, Stojković, Biljana, Savković, Uroš, Immonen, Elina, Tucić, Nikola, Lazarević, Jelica, Arnqvist, Göran, "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles" in Evolution, 71, no. 2 (2017):274-288,
https://doi.org/10.1111/evo.13109 . .

TY  - JOUR
AU  - Đorđević, Mirko
AU  - Stojković, Biljana
AU  - Savković, Uroš
AU  - Immonen, Elina
AU  - Tucić, Nikola
AU  - Lazarević, Jelica
AU  - Arnqvist, Göran
PY  - 2016
UR  - http://doi.wiley.com/10.1111/evo.13109
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2549
AB  - The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.
T2  - Evolution
T1  - Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles
DO  - 10.1111/evo.13109
ER  - 

@article{
author = "Đorđević, Mirko and Stojković, Biljana and Savković, Uroš and Immonen, Elina and Tucić, Nikola and Lazarević, Jelica and Arnqvist, Göran",
year = "2016",
abstract = "The role of mitochondrial DNA for the evolution of life-history traits remains debated. We examined mitonuclear effects on the activity of the multisubunit complex of the electron transport chain (ETC) involved in oxidative phosphorylation (OXPHOS) across lines of the seed beetle Acanthoscelides obtectus selected for a short (E) or a long (L) life for more than >160 generations. We constructed and phenotyped mitonuclear introgression lines, which allowed us to assess the independent effects of the evolutionary history of the nuclear and the mitochondrial genome. The nuclear genome was responsible for the largest share of divergence seen in ageing. However, the mitochondrial genome also had sizeable effects, which were sex-specific and expressed primarily as epistatic interactions with the nuclear genome. The effects of mitonuclear disruption were largely consistent with mitonuclear coadaptation. Variation in ETC activity explained a large proportion of variance in ageing and life-history traits and this multivariate relationship differed somewhat between the sexes. In conclusion, mitonuclear epistasis has played an important role in the laboratory evolution of ETC complex activity, ageing, and life histories and these are closely associated. The mitonuclear architecture of evolved differences in life-history traits and mitochondrial bioenergetics was sex-specific.",
journal = "Evolution",
title = "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles",
doi = "10.1111/evo.13109"
}

Đorđević, M., Stojković, B., Savković, U., Immonen, E., Tucić, N., Lazarević, J.,& Arnqvist, G.. (2016). Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution.
https://doi.org/10.1111/evo.13109

Đorđević M, Stojković B, Savković U, Immonen E, Tucić N, Lazarević J, Arnqvist G. Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles. in Evolution. 2016;.
doi:10.1111/evo.13109 .

Đorđević, Mirko, Stojković, Biljana, Savković, Uroš, Immonen, Elina, Tucić, Nikola, Lazarević, Jelica, Arnqvist, Göran, "Sex-specific mitonuclear epistasis and the evolution of mitochondrial bioenergetics, ageing, and life history in seed beetles" in Evolution (2016),
https://doi.org/10.1111/evo.13109 . .

TY  - JOUR
AU  - Savković, Uroš
AU  - Đorđević, Mirko
AU  - Šešlija Jovanović, Darka
AU  - Lazarević, Jelica
AU  - Tucić, Nikola
AU  - Stojković, Biljana
PY  - 2016
UR  - http://onlinelibrary.wiley.com/doi/10.1111/jeb.12831/abstract
UR  - http://www.ncbi.nlm.nih.gov/pubmed/26790127
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2925
AB  - Expansion of the host range in phytophagous insects depends on their ability to form an association with a novel plant through changes in host-related traits. Phenotypic plasticity has important effects on initial survival of individuals faced with a new plant, as well as on the courses of evolutionary change during long-term adaptation to novel conditions. Using experimental populations of the seed beetle that evolved on ancestral (common bean) or novel (chickpea) host and applying reciprocal transplant at both larval and adult stage on the alternative host plant, we studied the relationship between the initial (plastic) phases of host-shift and the subsequent stages of evolutionary divergence in life-history strategies between populations exposed to the host-shift process. After 48 generations, populations became well adapted to chickpea by evolving the life-history strategy with prolonged larval development, increased body mass, earlier reproduction, shorter lifespan and decreased plasticity of all traits compared with ancestral conditions. In chickpea-adapted beetles, negative fitness consequences of low plasticity of pre-adult development (revealed as severe decrease in egg-to-adult viability on beans) exhibited mismatch with positive effects of low plasticity (i.e. low host sensitivity) in oviposition and fecundity. In contrast, beetles adapted to the ancestral host showed high plasticity of developmental process, which enabled high larval survival on chickpea, whereas elevated plasticity in adult behaviour (i.e. high host sensitivity) resulted in delayed reproduction and decreased fecundity on chickpea. The analysis of population growth parameters revealed significant fluctuation during successive phases of the host-shift process in A. obtectus.
T2  - Journal of Evolutionary Biology
T1  - Experimentally induced host-shift changes life-history strategy in a seed beetle.
IS  - 4
VL  - 29
DO  - 10.1111/jeb.12831
SP  - 837
EP  - 47
ER  - 

@article{
author = "Savković, Uroš and Đorđević, Mirko and Šešlija Jovanović, Darka and Lazarević, Jelica and Tucić, Nikola and Stojković, Biljana",
year = "2016",
abstract = "Expansion of the host range in phytophagous insects depends on their ability to form an association with a novel plant through changes in host-related traits. Phenotypic plasticity has important effects on initial survival of individuals faced with a new plant, as well as on the courses of evolutionary change during long-term adaptation to novel conditions. Using experimental populations of the seed beetle that evolved on ancestral (common bean) or novel (chickpea) host and applying reciprocal transplant at both larval and adult stage on the alternative host plant, we studied the relationship between the initial (plastic) phases of host-shift and the subsequent stages of evolutionary divergence in life-history strategies between populations exposed to the host-shift process. After 48 generations, populations became well adapted to chickpea by evolving the life-history strategy with prolonged larval development, increased body mass, earlier reproduction, shorter lifespan and decreased plasticity of all traits compared with ancestral conditions. In chickpea-adapted beetles, negative fitness consequences of low plasticity of pre-adult development (revealed as severe decrease in egg-to-adult viability on beans) exhibited mismatch with positive effects of low plasticity (i.e. low host sensitivity) in oviposition and fecundity. In contrast, beetles adapted to the ancestral host showed high plasticity of developmental process, which enabled high larval survival on chickpea, whereas elevated plasticity in adult behaviour (i.e. high host sensitivity) resulted in delayed reproduction and decreased fecundity on chickpea. The analysis of population growth parameters revealed significant fluctuation during successive phases of the host-shift process in A. obtectus.",
journal = "Journal of Evolutionary Biology",
title = "Experimentally induced host-shift changes life-history strategy in a seed beetle.",
number = "4",
volume = "29",
doi = "10.1111/jeb.12831",
pages = "837-47"
}

Savković, U., Đorđević, M., Šešlija Jovanović, D., Lazarević, J., Tucić, N.,& Stojković, B.. (2016). Experimentally induced host-shift changes life-history strategy in a seed beetle.. in Journal of Evolutionary Biology, 29(4), 837-47.
https://doi.org/10.1111/jeb.12831

Savković U, Đorđević M, Šešlija Jovanović D, Lazarević J, Tucić N, Stojković B. Experimentally induced host-shift changes life-history strategy in a seed beetle.. in Journal of Evolutionary Biology. 2016;29(4):837-47.
doi:10.1111/jeb.12831 .

Savković, Uroš, Đorđević, Mirko, Šešlija Jovanović, Darka, Lazarević, Jelica, Tucić, Nikola, Stojković, Biljana, "Experimentally induced host-shift changes life-history strategy in a seed beetle." in Journal of Evolutionary Biology, 29, no. 4 (2016):837-47,
https://doi.org/10.1111/jeb.12831 . .