TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Budečević, Sanja
AU  - Ljubisavljević, Katarina
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6571
AB  - The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.
PB  - John Wiley and Sons
T2  - Journal of Anatomy
T1  - Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts
DO  - 10.1111/joa.14030
ER  - 

@article{
author = "Urošević, Aleksandar and Budečević, Sanja and Ljubisavljević, Katarina and Tomašević Kolarov, Nataša and Ajduković, Maja",
year = "2024",
abstract = "The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.",
publisher = "John Wiley and Sons",
journal = "Journal of Anatomy",
title = "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts",
doi = "10.1111/joa.14030"
}

Urošević, A., Budečević, S., Ljubisavljević, K., Tomašević Kolarov, N.,& Ajduković, M.. (2024). Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy
John Wiley and Sons..
https://doi.org/10.1111/joa.14030

Urošević A, Budečević S, Ljubisavljević K, Tomašević Kolarov N, Ajduković M. Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy. 2024;.
doi:10.1111/joa.14030 .

Urošević, Aleksandar, Budečević, Sanja, Ljubisavljević, Katarina, Tomašević Kolarov, Nataša, Ajduković, Maja, "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts" in Journal of Anatomy (2024),
https://doi.org/10.1111/joa.14030 . .

TY  - JOUR
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Prokić, Marko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6580
AB  - The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?
VL  - 278
DO  - 10.1016/j.cbpc.2024.109859
SP  - 109859
ER  - 

@article{
author = "Gavrilović, Branka and Despotović, Svetlana and Petrović, Tamara and Radovanović, Tijana and Gavrić, Jelena and Mirč, Marko and Anđelković, Marko and Vukov, Tanja and Tomašević Kolarov, Nataša and Prokić, Marko",
year = "2024",
abstract = "The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?",
volume = "278",
doi = "10.1016/j.cbpc.2024.109859",
pages = "109859"
}

Gavrilović, B., Despotović, S., Petrović, T., Radovanović, T., Gavrić, J., Mirč, M., Anđelković, M., Vukov, T., Tomašević Kolarov, N.,& Prokić, M.. (2024). Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 278, 109859.
https://doi.org/10.1016/j.cbpc.2024.109859

Gavrilović B, Despotović S, Petrović T, Radovanović T, Gavrić J, Mirč M, Anđelković M, Vukov T, Tomašević Kolarov N, Prokić M. Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2024;278:109859.
doi:10.1016/j.cbpc.2024.109859 .

Gavrilović, Branka, Despotović, Svetlana, Petrović, Tamara, Radovanović, Tijana, Gavrić, Jelena, Mirč, Marko, Anđelković, Marko, Vukov, Tanja, Tomašević Kolarov, Nataša, Prokić, Marko, "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 278 (2024):109859,
https://doi.org/10.1016/j.cbpc.2024.109859 . .

TY  - JOUR
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Gavrić, Jelena
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Prokić, Marko
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6579
AB  - The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?
VL  - 278
DO  - 10.1016/j.cbpc.2024.109859
SP  - 109859
ER  - 

@article{
author = "Gavrilović, Branka and Despotović, Svetlana and Petrović, Tamara and Radovanović, Tijana and Gavrić, Jelena and Mirč, Marko and Anđelković, Marko and Vukov, Tanja and Tomašević Kolarov, Nataša and Prokić, Marko",
year = "2024",
abstract = "The effect of anesthesia/euthanasia with ethyl 3-aminobenzoate methanesulfonate (MS-222) on the oxidative status of Hyla arborea tadpoles was examined to determine whether the use of the anesthetic can confound the experimental results of the oxidative stress-based investigation. The experiment was conducted on two groups of tadpoles reared at different temperatures to produce differences in antioxidant capacity between the groups. After development at different temperatures (20 °C and 25 °C), the animals were exposed to different concentrations of MS-222 (0, 0.1, 1, and 5 g/L) for 15 min. The higher temperature decreased catalase activity, glutathione and protein carbonyl levels and increased glutathione reductase activity. The glutathione level and glutathione/thiol-related parameters were significantly changed after MS-222 exposure. However, individuals from the different temperature groups responded differently to the tested anesthetic, pointing to the possible influence of the initial levels of antioxidant capacity. The analysis of the interaction between the factors (temperature and MS-222) confirmed that the anesthetic can confound the results regarding the effects of temperature on the oxidative status parameters. The concentration of 0.1 g/L MS-222 had the lowest influence on the alterations in oxidative status and the results of the effect of temperature. A brief review of the current literature on the use of MS-222 in tadpoles made clear the absence of precise information on anesthetic concentration and exposure time. Similar studies should be repeated and extended to other amphibian species and other factors of interest to provide better guidance on tadpole anesthesia/euthanasia for future experiments that consider oxidative status parameters.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?",
volume = "278",
doi = "10.1016/j.cbpc.2024.109859",
pages = "109859"
}

Gavrilović, B., Despotović, S., Petrović, T., Radovanović, T., Gavrić, J., Mirč, M., Anđelković, M., Vukov, T., Tomašević Kolarov, N.,& Prokić, M.. (2024). Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
Elsevier Inc.., 278, 109859.
https://doi.org/10.1016/j.cbpc.2024.109859

Gavrilović B, Despotović S, Petrović T, Radovanović T, Gavrić J, Mirč M, Anđelković M, Vukov T, Tomašević Kolarov N, Prokić M. Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2024;278:109859.
doi:10.1016/j.cbpc.2024.109859 .

Gavrilović, Branka, Despotović, Svetlana, Petrović, Tamara, Radovanović, Tijana, Gavrić, Jelena, Mirč, Marko, Anđelković, Marko, Vukov, Tanja, Tomašević Kolarov, Nataša, Prokić, Marko, "Does the anesthetic tricaine methanesulfonate (MS-222) distort oxidative status parameters in tadpoles?" in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 278 (2024):109859,
https://doi.org/10.1016/j.cbpc.2024.109859 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Budečević, Sanja
AU  - Ljubisavljević, Katarina
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6571
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6572
AB  - The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.
PB  - John Wiley and Sons
T2  - Journal of Anatomy
T1  - Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts
DO  - 10.1111/joa.14030
ER  - 

@article{
author = "Urošević, Aleksandar and Budečević, Sanja and Ljubisavljević, Katarina and Tomašević Kolarov, Nataša and Ajduković, Maja",
year = "2024",
abstract = "The modular organization of tetrapod paired limbs and girdles, influenced by the expression of Hox genes is one of the primary driving forces of the evolution of animal locomotion. The increased morphological diversification of the paired limbs is correlated with reduced between-limb covariation, while correlation within the elements is usually higher than between the elements. The tailed amphibians, such as Lissotriton newts, have a biphasic lifestyle with aquatic and terrestrial environments imposing different constraints on limb skeleton. By employing the methods of computerized microtomography and 3D geometric morphometrics, we explored the pattern of morphological variation, disparity, modularity and morphological integration in the proximal parts of the anterior limbs of six species of Eurasian small bodied newts. Although the species significantly differ in limb shape, there is a great overlap in morphology of scapula and humerus, and there are no differences in morphological disparity. For the scapula, the shape differences related to the duration of the aquatic period are in length, depth and curvature. The shape of the humerus is not affected by the length of aquatic period, and shape differences between the species are related to robustness of the body. The length of aquatic period has statistically supported phylogenetic signal. The scapula and humerus are structures of varying modularity. For the humerus, the strongest support on the phylogenetic level was for the capitulum/shaft hypothesis, which can also be interpreted as functional modularity. For the scapula, the greatest support was for the antero-posterior hypothesis of modularity in case of Lissotriton vulgaris, which can be explained by different functional roles and muscle insertion patterns, while there was no phylogenetic modularity. The modularity patterns seem to correspond with the general tetrapod pattern, with modularity being more pronounced in the distal structure. The future research should include more salamandrid taxa with different habitat preferences and both adult and larval stages, in order to explore how size, phylogeny and ecology affect the morphology and covariation patterns of limbs.",
publisher = "John Wiley and Sons",
journal = "Journal of Anatomy",
title = "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts",
doi = "10.1111/joa.14030"
}

Urošević, A., Budečević, S., Ljubisavljević, K., Tomašević Kolarov, N.,& Ajduković, M.. (2024). Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy
John Wiley and Sons..
https://doi.org/10.1111/joa.14030

Urošević A, Budečević S, Ljubisavljević K, Tomašević Kolarov N, Ajduković M. Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts. in Journal of Anatomy. 2024;.
doi:10.1111/joa.14030 .

Urošević, Aleksandar, Budečević, Sanja, Ljubisavljević, Katarina, Tomašević Kolarov, Nataša, Ajduković, Maja, "Morphological variation, modularity and integration in the scapula and humerus of Lissotriton newts" in Journal of Anatomy (2024),
https://doi.org/10.1111/joa.14030 . .

TY  - CONF
AU  - Kijanović, Ana
AU  - Vukov, Tanja
AU  - Mirč, Marko
AU  - Mitrović, Aleksandar
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Tomašević Kolarov, Nataša
PY  - 2023
UR  - https://www.sebiology.org/events/seb-centenary-conference-2023/abstracts.html
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6060
AB  - Amphibian species that inhabit temporary ponds for reproduction maximize larval growth under favorable conditions and accelerate their development to undergo rapid metamorphosis under stressful conditions such as pond drying. Corticosterone controls development, metabolism, and growth, and has an invaluable role in anuran metamorphosis under stress conditions. In this study, we evaluated whether the whole-body corticosterone (CORT) level is related to drying conditions in species that cannot accelerate the developmental rate in response to pond drying. Specifically, we investigated the effects of different water levels in combination with exogenous CORT and corticosteroid synthesis inhibitor metyrapone (treatments: high water level, high water level with exogenous CORT, low water level, low water level with metyrapone) on the whole-body corticosterone (at prometamorphosis and metamorphic climax), life history and morphological traits at the metamorphic climax. We found that these conditions did not alter the whole-body content of CORT and the developmental rate in treatments, although low water levels and exogenous CORT in high water level negatively affected other life history traits and tail shape. Individuals from a high water level with exogenous CORT had the smallest body size and mass and changed tail shape at metamorphosis, while changes in life history traits did not affect the tail shape in the other treatments. Our findings indicate that the absence of developmental response (i.e. canalized development) in the timing of metamorphosis of B. variegata may be explained by a modification of endocrine regulation but further studies that would include closely related species are required.
PB  - Society for Experimental Biology
C3  - Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK
T1  - Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs
SP  - 8
EP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6060
ER  - 

@conference{
author = "Kijanović, Ana and Vukov, Tanja and Mirč, Marko and Mitrović, Aleksandar and Prokić, Marko and Petrović, Tamara and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Tomašević Kolarov, Nataša",
year = "2023",
abstract = "Amphibian species that inhabit temporary ponds for reproduction maximize larval growth under favorable conditions and accelerate their development to undergo rapid metamorphosis under stressful conditions such as pond drying. Corticosterone controls development, metabolism, and growth, and has an invaluable role in anuran metamorphosis under stress conditions. In this study, we evaluated whether the whole-body corticosterone (CORT) level is related to drying conditions in species that cannot accelerate the developmental rate in response to pond drying. Specifically, we investigated the effects of different water levels in combination with exogenous CORT and corticosteroid synthesis inhibitor metyrapone (treatments: high water level, high water level with exogenous CORT, low water level, low water level with metyrapone) on the whole-body corticosterone (at prometamorphosis and metamorphic climax), life history and morphological traits at the metamorphic climax. We found that these conditions did not alter the whole-body content of CORT and the developmental rate in treatments, although low water levels and exogenous CORT in high water level negatively affected other life history traits and tail shape. Individuals from a high water level with exogenous CORT had the smallest body size and mass and changed tail shape at metamorphosis, while changes in life history traits did not affect the tail shape in the other treatments. Our findings indicate that the absence of developmental response (i.e. canalized development) in the timing of metamorphosis of B. variegata may be explained by a modification of endocrine regulation but further studies that would include closely related species are required.",
publisher = "Society for Experimental Biology",
journal = "Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK",
title = "Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs",
pages = "8-9",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6060"
}

Kijanović, A., Vukov, T., Mirč, M., Mitrović, A., Prokić, M., Petrović, T., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J.,& Tomašević Kolarov, N.. (2023). Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs. in Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK
Society for Experimental Biology., 8-9.
https://hdl.handle.net/21.15107/rcub_ibiss_6060

Kijanović A, Vukov T, Mirč M, Mitrović A, Prokić M, Petrović T, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Tomašević Kolarov N. Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs. in Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK. 2023;:8-9.
https://hdl.handle.net/21.15107/rcub_ibiss_6060 .

Kijanović, Ana, Vukov, Tanja, Mirč, Marko, Mitrović, Aleksandar, Prokić, Marko, Petrović, Tamara, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Tomašević Kolarov, Nataša, "Pond drying conditions do not alter whole-body corticosterone content and metamorphic time of yellow-bellied toad (Bombina variegata) metamorphs" in Abstract book: SEB Centenary Conference 1923-2023: Celebrating Success and Shaping the Future; 2023 Jul 4-7; Edinburgh, UK (2023):8-9,
https://hdl.handle.net/21.15107/rcub_ibiss_6060 .

TY  - JOUR
AU  - Mirč, Marko
AU  - Vukov, Tanja
AU  - Kijanović, Ana
AU  - Tomašević Kolarov, Nataša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5489
AB  - Amphibian tadpoles are capable of avoiding threats (predators, uv radiation, etc.) through changes in coloration, behavior, and shape. In this paper, we tested how quickly European tree frog (Hyla arborea) tadpoles can change body pigmentation to achieve crypsis and whether color change is reversible. Additionally, we tested how different environmental background colorations affect the body length, shape, and ontogenetic trajectories of tadpoles. We also analyzed if tadpoles can relate to their coloration and choose the appropriate background to enhance crypsis. For this purpose, we reared tadpoles on white and black backgrounds for 36 days. Halfway through the experiment, half of the tadpoles from each treatment were placed on the alternative background. Our results suggest that H. arborea tadpoles are capable of rapidly responding to color changes in their environment, however, color-matching with the white background is poor. These quick color changes are reversible. Rearing in different background coloration and rapid color changes do not affect tadpoles' length variation but affect tadpoles' shape. Tadpoles introduced to the white background at the start of the experiment developed deeper tail fins and more pronounced snouts. We also found that H. arborea tadpoles actively choose an appropriate background to achieve maximum crypsis. This study represents the basis for the future analysis of adaptive coloration in tadpoles as it has a very complex function in anurans.
PB  - Brill Academic Publishers
T2  - Contributions to Zoology
T1  - Effects of background color on pigmentation, morphological traits, and behavior in the European tree frog (Hyla arborea, Hylidae, Anura) tadpoles
DO  - 10.1163/18759866-bja10040
ER  - 

@article{
author = "Mirč, Marko and Vukov, Tanja and Kijanović, Ana and Tomašević Kolarov, Nataša",
year = "2023",
abstract = "Amphibian tadpoles are capable of avoiding threats (predators, uv radiation, etc.) through changes in coloration, behavior, and shape. In this paper, we tested how quickly European tree frog (Hyla arborea) tadpoles can change body pigmentation to achieve crypsis and whether color change is reversible. Additionally, we tested how different environmental background colorations affect the body length, shape, and ontogenetic trajectories of tadpoles. We also analyzed if tadpoles can relate to their coloration and choose the appropriate background to enhance crypsis. For this purpose, we reared tadpoles on white and black backgrounds for 36 days. Halfway through the experiment, half of the tadpoles from each treatment were placed on the alternative background. Our results suggest that H. arborea tadpoles are capable of rapidly responding to color changes in their environment, however, color-matching with the white background is poor. These quick color changes are reversible. Rearing in different background coloration and rapid color changes do not affect tadpoles' length variation but affect tadpoles' shape. Tadpoles introduced to the white background at the start of the experiment developed deeper tail fins and more pronounced snouts. We also found that H. arborea tadpoles actively choose an appropriate background to achieve maximum crypsis. This study represents the basis for the future analysis of adaptive coloration in tadpoles as it has a very complex function in anurans.",
publisher = "Brill Academic Publishers",
journal = "Contributions to Zoology",
title = "Effects of background color on pigmentation, morphological traits, and behavior in the European tree frog (Hyla arborea, Hylidae, Anura) tadpoles",
doi = "10.1163/18759866-bja10040"
}

Mirč, M., Vukov, T., Kijanović, A.,& Tomašević Kolarov, N.. (2023). Effects of background color on pigmentation, morphological traits, and behavior in the European tree frog (Hyla arborea, Hylidae, Anura) tadpoles. in Contributions to Zoology
Brill Academic Publishers..
https://doi.org/10.1163/18759866-bja10040

Mirč M, Vukov T, Kijanović A, Tomašević Kolarov N. Effects of background color on pigmentation, morphological traits, and behavior in the European tree frog (Hyla arborea, Hylidae, Anura) tadpoles. in Contributions to Zoology. 2023;.
doi:10.1163/18759866-bja10040 .

Mirč, Marko, Vukov, Tanja, Kijanović, Ana, Tomašević Kolarov, Nataša, "Effects of background color on pigmentation, morphological traits, and behavior in the European tree frog (Hyla arborea, Hylidae, Anura) tadpoles" in Contributions to Zoology (2023),
https://doi.org/10.1163/18759866-bja10040 . .

TY  - JOUR
AU  - Kijanović, Ana
AU  - Vukov, Tanja
AU  - Mirč, Marko
AU  - Krizmanić, Imre
AU  - Tomašević Kolarov, Nataša
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5468
AB  - Many amphibian species inhabit temporary aquatic habitats for reproduction to
maximize larval growth under favorable conditions, but accelerate developmental
rate to rapidly undergo metamorphosis under stress conditions when high desicca-
tion risk exists. Desiccation affects different aspects of amphibian biology such as
developmental rate, body size and mass, and growth rate. We studied the responses
of yellow-bellied toad (Bombina variegata) to different water levels (constant high,
decreasing water level, and constant low water level) in the laboratory using indi-
viduals collected from natural ponds. To assess the effects of experimental pond
drying, we investigated the effects on life-history and morphological traits at meta-
morphosis as well as carry-over effects on post-metamorphic traits and locomotor
performances. We found that B. variegata cannot accelerate developmental rate in
response to pond drying. The metamorphs from decreasing and constant low water
levels had similar size, mass, growth rate and body condition indices at metamorphosis, and all these traits were lower than in the constant high water level treatment. These changes in life-history traits at metamorphosis did not affect tail shape although they had carry-over effects on life-history and morphological traits after metamorphosis. Post-metamorphic individuals reared in desiccation treatments had
similar but smaller lengths of femur and tibiofibula, but these changes did not have
negative consequences on jumping performances. We show that responsiveness to
pond drying of B. variegata is probably associated with maximum growth rate in favorable conditions and inability to increase growth rate as well as developmental rate in desiccation conditions. This resulted in prolonged developmental time to reach the minimum size and mass for metamorphosis.
PB  - John Wiley and Sons Inc
T2  - Journal of Zoology
T1  - Inability of yellow-bellied toad to accelerate metamorphosis in desiccation conditions
DO  - 10.1111/jzo.13056
ER  - 

@article{
author = "Kijanović, Ana and Vukov, Tanja and Mirč, Marko and Krizmanić, Imre and Tomašević Kolarov, Nataša",
year = "2023",
abstract = "Many amphibian species inhabit temporary aquatic habitats for reproduction to
maximize larval growth under favorable conditions, but accelerate developmental
rate to rapidly undergo metamorphosis under stress conditions when high desicca-
tion risk exists. Desiccation affects different aspects of amphibian biology such as
developmental rate, body size and mass, and growth rate. We studied the responses
of yellow-bellied toad (Bombina variegata) to different water levels (constant high,
decreasing water level, and constant low water level) in the laboratory using indi-
viduals collected from natural ponds. To assess the effects of experimental pond
drying, we investigated the effects on life-history and morphological traits at meta-
morphosis as well as carry-over effects on post-metamorphic traits and locomotor
performances. We found that B. variegata cannot accelerate developmental rate in
response to pond drying. The metamorphs from decreasing and constant low water
levels had similar size, mass, growth rate and body condition indices at metamorphosis, and all these traits were lower than in the constant high water level treatment. These changes in life-history traits at metamorphosis did not affect tail shape although they had carry-over effects on life-history and morphological traits after metamorphosis. Post-metamorphic individuals reared in desiccation treatments had
similar but smaller lengths of femur and tibiofibula, but these changes did not have
negative consequences on jumping performances. We show that responsiveness to
pond drying of B. variegata is probably associated with maximum growth rate in favorable conditions and inability to increase growth rate as well as developmental rate in desiccation conditions. This resulted in prolonged developmental time to reach the minimum size and mass for metamorphosis.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Zoology",
title = "Inability of yellow-bellied toad to accelerate metamorphosis in desiccation conditions",
doi = "10.1111/jzo.13056"
}

Kijanović, A., Vukov, T., Mirč, M., Krizmanić, I.,& Tomašević Kolarov, N.. (2023). Inability of yellow-bellied toad to accelerate metamorphosis in desiccation conditions. in Journal of Zoology
John Wiley and Sons Inc..
https://doi.org/10.1111/jzo.13056

Kijanović A, Vukov T, Mirč M, Krizmanić I, Tomašević Kolarov N. Inability of yellow-bellied toad to accelerate metamorphosis in desiccation conditions. in Journal of Zoology. 2023;.
doi:10.1111/jzo.13056 .

Kijanović, Ana, Vukov, Tanja, Mirč, Marko, Krizmanić, Imre, Tomašević Kolarov, Nataša, "Inability of yellow-bellied toad to accelerate metamorphosis in desiccation conditions" in Journal of Zoology (2023),
https://doi.org/10.1111/jzo.13056 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Petrović, Tamara
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Vukov, Tanja
AU  - Prokić, Marko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5464
AB  - Background Urban development results in habitat destruction, affecting populations of amphibians, the most fragile
group of vertebrates. With changes in the environment, these animals become more exposed to light and predators.
To enhance their chances of survival, they display plasticity of body coloration. Aside from adaptive benefits,
animals exhibiting background matching meet the energetic costs and restrictions of changing body tones. To study
the physiological consequences of Hyla arborea tadpole adaptation to background color, we followed oxidative stress
parameters after rearing larvae on a constant background (black/white) and after changing the background color.
Results Larvae cultivated for 20 days on constant substrate color exhibited differences in body coloration but without
differences in lipid peroxidation (LPO) concentration between dark and pale individuals, suggesting that coloration
investment during this period did not induce higher oxidative damage in darker tadpoles. Prolonged exposure
of larvae (37 days) to a dark habitat increased antioxidative system defense and LPO concentrations, compared to
animals reared permanently in the white surroundings. The positive correlation of oxidative damage with color
intensity of individuals points to the physiological consequences of higher investment in the number of pigment cells
necessary for dark pigmentation. In individuals faced with non-matching background and change in body coloration,
defense system declined and LPO occurred relative to individuals cultivated in white habitat.
Conclusion Here, we have pointed to consequences related to background matching and stress that amphibians
experienced during chromatic adaptations. Background color change causes a complex physiological response
affecting the antioxidative defense parameters. This investigation elucidates the accompanying cost of amphibiansʼ
adjustment to an altered environment.
PB  - London: BMC
T2  - Frontiers in Zoology
T1  - What coloration brings: Implications of background adaptation to oxidative stress in anurans
IS  - 1
VL  - 20
DO  - 10.1186/s12983-023-00486-z
SP  - 6
ER  - 

@article{
author = "Radovanović, Tijana and Petrović, Tamara and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Vukov, Tanja and Prokić, Marko",
year = "2023",
abstract = "Background Urban development results in habitat destruction, affecting populations of amphibians, the most fragile
group of vertebrates. With changes in the environment, these animals become more exposed to light and predators.
To enhance their chances of survival, they display plasticity of body coloration. Aside from adaptive benefits,
animals exhibiting background matching meet the energetic costs and restrictions of changing body tones. To study
the physiological consequences of Hyla arborea tadpole adaptation to background color, we followed oxidative stress
parameters after rearing larvae on a constant background (black/white) and after changing the background color.
Results Larvae cultivated for 20 days on constant substrate color exhibited differences in body coloration but without
differences in lipid peroxidation (LPO) concentration between dark and pale individuals, suggesting that coloration
investment during this period did not induce higher oxidative damage in darker tadpoles. Prolonged exposure
of larvae (37 days) to a dark habitat increased antioxidative system defense and LPO concentrations, compared to
animals reared permanently in the white surroundings. The positive correlation of oxidative damage with color
intensity of individuals points to the physiological consequences of higher investment in the number of pigment cells
necessary for dark pigmentation. In individuals faced with non-matching background and change in body coloration,
defense system declined and LPO occurred relative to individuals cultivated in white habitat.
Conclusion Here, we have pointed to consequences related to background matching and stress that amphibians
experienced during chromatic adaptations. Background color change causes a complex physiological response
affecting the antioxidative defense parameters. This investigation elucidates the accompanying cost of amphibiansʼ
adjustment to an altered environment.",
publisher = "London: BMC",
journal = "Frontiers in Zoology",
title = "What coloration brings: Implications of background adaptation to oxidative stress in anurans",
number = "1",
volume = "20",
doi = "10.1186/s12983-023-00486-z",
pages = "6"
}

Radovanović, T., Petrović, T., Gavrilović, B., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Vukov, T.,& Prokić, M.. (2023). What coloration brings: Implications of background adaptation to oxidative stress in anurans. in Frontiers in Zoology
London: BMC., 20(1), 6.
https://doi.org/10.1186/s12983-023-00486-z

Radovanović T, Petrović T, Gavrilović B, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Vukov T, Prokić M. What coloration brings: Implications of background adaptation to oxidative stress in anurans. in Frontiers in Zoology. 2023;20(1):6.
doi:10.1186/s12983-023-00486-z .

Radovanović, Tijana, Petrović, Tamara, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Vukov, Tanja, Prokić, Marko, "What coloration brings: Implications of background adaptation to oxidative stress in anurans" in Frontiers in Zoology, 20, no. 1 (2023):6,
https://doi.org/10.1186/s12983-023-00486-z . .

TY  - JOUR
AU  - Milošević-Zlatanović, Svetlana
AU  - Vukov, Tanja
AU  - Chovancová, Gabriela
AU  - Anderwald, Pia
AU  - Corlatti, Luca
AU  - Tomašević Kolarov, Nataša
PY  - 2022
UR  - https://link.springer.com/10.1007/s10914-022-09644-2
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5354
AB  - Covariance structure patterning of the phenotype can provide important insights into the evolution of forms. In this paper, we characterize the sex-specific phenotypic structure of cranial morphology in the four subspecies of chamois Rupicapra rupicapra (balcanica, carpatica, tatrica and rupicapra). We investigate variation in 18 craniometric characters of 360 adults chamois. Compared to the other subspecies, tatrica showed a highly integrated cranium. The cranium of carpatica and balcanica evolved in a more modular fashion, with most conspicuous nasal and oral modules. Females showed stronger correlations among cranial traits than males, suggesting higher potential for adaptations to new environmental conditions in the latter, matching sex-specific behavioral features. Subspecies rupicapra and tatrica showed contrasting levels of overall integration, followed by low levels of modularity, with sexual differences only in rupicapra. Our results suggest that, patterns and magnitudes of correlations among skeletal elements of the skull can evolve within a species through habitat and sexual selection.
T2  - Journal of Mammalian Evolution
T1  - Cranial integration and modularity in chamois: The effects of subspecies and sex
DO  - 10.1007/s10914-022-09644-2
ER  - 

@article{
author = "Milošević-Zlatanović, Svetlana and Vukov, Tanja and Chovancová, Gabriela and Anderwald, Pia and Corlatti, Luca and Tomašević Kolarov, Nataša",
year = "2022",
abstract = "Covariance structure patterning of the phenotype can provide important insights into the evolution of forms. In this paper, we characterize the sex-specific phenotypic structure of cranial morphology in the four subspecies of chamois Rupicapra rupicapra (balcanica, carpatica, tatrica and rupicapra). We investigate variation in 18 craniometric characters of 360 adults chamois. Compared to the other subspecies, tatrica showed a highly integrated cranium. The cranium of carpatica and balcanica evolved in a more modular fashion, with most conspicuous nasal and oral modules. Females showed stronger correlations among cranial traits than males, suggesting higher potential for adaptations to new environmental conditions in the latter, matching sex-specific behavioral features. Subspecies rupicapra and tatrica showed contrasting levels of overall integration, followed by low levels of modularity, with sexual differences only in rupicapra. Our results suggest that, patterns and magnitudes of correlations among skeletal elements of the skull can evolve within a species through habitat and sexual selection.",
journal = "Journal of Mammalian Evolution",
title = "Cranial integration and modularity in chamois: The effects of subspecies and sex",
doi = "10.1007/s10914-022-09644-2"
}

Milošević-Zlatanović, S., Vukov, T., Chovancová, G., Anderwald, P., Corlatti, L.,& Tomašević Kolarov, N.. (2022). Cranial integration and modularity in chamois: The effects of subspecies and sex. in Journal of Mammalian Evolution.
https://doi.org/10.1007/s10914-022-09644-2

Milošević-Zlatanović S, Vukov T, Chovancová G, Anderwald P, Corlatti L, Tomašević Kolarov N. Cranial integration and modularity in chamois: The effects of subspecies and sex. in Journal of Mammalian Evolution. 2022;.
doi:10.1007/s10914-022-09644-2 .

Milošević-Zlatanović, Svetlana, Vukov, Tanja, Chovancová, Gabriela, Anderwald, Pia, Corlatti, Luca, Tomašević Kolarov, Nataša, "Cranial integration and modularity in chamois: The effects of subspecies and sex" in Journal of Mammalian Evolution (2022),
https://doi.org/10.1007/s10914-022-09644-2 . .

TY  - CONF
AU  - Mirč, Marko
AU  - Vukov, Tanja
AU  - Kijanović, Ana
AU  - Tomašević Kolarov, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5316
AB  - Amphibian tadpoles can avoid threats through defensive coloration and
defensive behavior. Another response to the exposure to threats is shape modification.
We studied if and how quickly can Hyla arborea tadpoles achieve crypsis, by changing
skin pigmentation, when introduced to environments with different background
colorations. We also analyzed the reversibility of the skin color change and how
different backgrounds affect tadpole body length, shape, and ontogenetic pathways. In
addition, we tested if tadpoles can perceive their own coloration and actively choose
an appropriate background. For this purpose, we reared tadpoles on white and black
backgrounds. Half of the tadpoles from each treatment were switched to the alternative
background halfway through the experiment. Coloration and length data were
collected six times during the experiment, while data for shape and ontogenetic
pathways analysis were collected twice (before the switch and at the end of the
experiment). The behavioral test was performed at the end of the experiment. Our
results suggest that H. arborea tadpoles can rapidly respond to the changes in the
environmental background and this color change is reversible, but adaptation to the
white background is not that good. Even though some physiological costs of rapid color
change, especially in switched treatments, surely exist, according to our results this
stress is not great enough to be expressed in variation of tadpoles’ body length.
However, some shape differences between treatments were evident. Tadpoles who
were initially introduced in the white background had deeper tail fins and more
pronounced snouts, a typical shape response when predation risk is perceived. Our
results, also suggest that H. arborea tadpoles can recognize their own coloration and
actively choose appropriate surroundings to maximize crypsis. This study represents
the basis for the future analysis of adaptive coloration as it has a very complex function
in anurans.
PB  - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, Belgrade, Serbia
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - How good are European tree frog (Hyla arborea) tadpoles in hiding?
SP  - 110
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5316
ER  - 

@conference{
author = "Mirč, Marko and Vukov, Tanja and Kijanović, Ana and Tomašević Kolarov, Nataša",
year = "2022",
abstract = "Amphibian tadpoles can avoid threats through defensive coloration and
defensive behavior. Another response to the exposure to threats is shape modification.
We studied if and how quickly can Hyla arborea tadpoles achieve crypsis, by changing
skin pigmentation, when introduced to environments with different background
colorations. We also analyzed the reversibility of the skin color change and how
different backgrounds affect tadpole body length, shape, and ontogenetic pathways. In
addition, we tested if tadpoles can perceive their own coloration and actively choose
an appropriate background. For this purpose, we reared tadpoles on white and black
backgrounds. Half of the tadpoles from each treatment were switched to the alternative
background halfway through the experiment. Coloration and length data were
collected six times during the experiment, while data for shape and ontogenetic
pathways analysis were collected twice (before the switch and at the end of the
experiment). The behavioral test was performed at the end of the experiment. Our
results suggest that H. arborea tadpoles can rapidly respond to the changes in the
environmental background and this color change is reversible, but adaptation to the
white background is not that good. Even though some physiological costs of rapid color
change, especially in switched treatments, surely exist, according to our results this
stress is not great enough to be expressed in variation of tadpoles’ body length.
However, some shape differences between treatments were evident. Tadpoles who
were initially introduced in the white background had deeper tail fins and more
pronounced snouts, a typical shape response when predation risk is perceived. Our
results, also suggest that H. arborea tadpoles can recognize their own coloration and
actively choose appropriate surroundings to maximize crypsis. This study represents
the basis for the future analysis of adaptive coloration as it has a very complex function
in anurans.",
publisher = "Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, Belgrade, Serbia, Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "How good are European tree frog (Hyla arborea) tadpoles in hiding?",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5316"
}

Mirč, M., Vukov, T., Kijanović, A.,& Tomašević Kolarov, N.. (2022). How good are European tree frog (Hyla arborea) tadpoles in hiding?. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, Belgrade, Serbia., 110.
https://hdl.handle.net/21.15107/rcub_ibiss_5316

Mirč M, Vukov T, Kijanović A, Tomašević Kolarov N. How good are European tree frog (Hyla arborea) tadpoles in hiding?. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:110.
https://hdl.handle.net/21.15107/rcub_ibiss_5316 .

Mirč, Marko, Vukov, Tanja, Kijanović, Ana, Tomašević Kolarov, Nataša, "How good are European tree frog (Hyla arborea) tadpoles in hiding?" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):110,
https://hdl.handle.net/21.15107/rcub_ibiss_5316 .

TY  - CONF
AU  - Kijanović, Ana
AU  - Vukov, Tanja
AU  - Mirč, Marko
AU  - Mitrović, Aleksandar
AU  - Prokić, Marko
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Petrović, Tamara
AU  - Tomašević Kolarov, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5250
AB  - Many amphibian species inhabit temporary aquatic habitats for reproduction to
maximize larval growth under favorable conditions but accelerate developmental rate
to rapidly undergo metamorphosis under stress conditions when high desiccation risk
exists. Corticosterone, one of the most important vertebrate stress hormone controls
development, metabolism, and skeletal growth and together with thyroid hormone
have a critical role in anuran metamorphosis under stress conditions. Here we
compare the hormonal variation of corticosterone (CORT) in the yellow-bellied toad
Bombina variegata tadpoles in response to different water levels (constant highcontrol, constant low, constant high+exogenous CORT) and the corticosterone
inhibitor- metyrapone (MET) (treatment constant low water level+MET). Also, we
investigated the effects of CORT and MET on life-history and morphological traits at
metamorphosis. We found that B. variegata tadpoles cannot accelerate the
developmental rate in response to pond drying. The constant low water level did not
alter whole-body content of CORT and it did not significantly differ from high water
level treatment with exogenous CORT. However, individuals from high water level with
exogenous CORT have prolonged development, the highest mortality rate, the smallest
body size and mass, and the widest but the shortest tails at metamorphosis. Constant
low water level with MET decreased the whole-body content of CORT and it was
significantly different between all groups, but it did not affect the developmental rate
and tail shape although these tadpoles have intermediate size and mass between high
water levels with exogenous CORT and control. We show that responsiveness to pond
drying of Bombina variegata and its short larval period is probably associated with
modified endocrine signaling pathway that control tadpole metamorphosis.
Constitutively high whole-body content of CORT which was unaltered by pond drying
leads us to conclusion that Bombina variegata development may be canalized but more
research is needed.
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Desiccation stress response of Bombina variegata tadpoles
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5250
ER  - 

@conference{
author = "Kijanović, Ana and Vukov, Tanja and Mirč, Marko and Mitrović, Aleksandar and Prokić, Marko and Radovanović, Tijana and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Petrović, Tamara and Tomašević Kolarov, Nataša",
year = "2022",
abstract = "Many amphibian species inhabit temporary aquatic habitats for reproduction to
maximize larval growth under favorable conditions but accelerate developmental rate
to rapidly undergo metamorphosis under stress conditions when high desiccation risk
exists. Corticosterone, one of the most important vertebrate stress hormone controls
development, metabolism, and skeletal growth and together with thyroid hormone
have a critical role in anuran metamorphosis under stress conditions. Here we
compare the hormonal variation of corticosterone (CORT) in the yellow-bellied toad
Bombina variegata tadpoles in response to different water levels (constant highcontrol, constant low, constant high+exogenous CORT) and the corticosterone
inhibitor- metyrapone (MET) (treatment constant low water level+MET). Also, we
investigated the effects of CORT and MET on life-history and morphological traits at
metamorphosis. We found that B. variegata tadpoles cannot accelerate the
developmental rate in response to pond drying. The constant low water level did not
alter whole-body content of CORT and it did not significantly differ from high water
level treatment with exogenous CORT. However, individuals from high water level with
exogenous CORT have prolonged development, the highest mortality rate, the smallest
body size and mass, and the widest but the shortest tails at metamorphosis. Constant
low water level with MET decreased the whole-body content of CORT and it was
significantly different between all groups, but it did not affect the developmental rate
and tail shape although these tadpoles have intermediate size and mass between high
water levels with exogenous CORT and control. We show that responsiveness to pond
drying of Bombina variegata and its short larval period is probably associated with
modified endocrine signaling pathway that control tadpole metamorphosis.
Constitutively high whole-body content of CORT which was unaltered by pond drying
leads us to conclusion that Bombina variegata development may be canalized but more
research is needed.",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Desiccation stress response of Bombina variegata tadpoles",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5250"
}

Kijanović, A., Vukov, T., Mirč, M., Mitrović, A., Prokić, M., Radovanović, T., Gavrilović, B., Despotović, S., Gavrić, J., Petrović, T.,& Tomašević Kolarov, N.. (2022). Desiccation stress response of Bombina variegata tadpoles. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 158.
https://hdl.handle.net/21.15107/rcub_ibiss_5250

Kijanović A, Vukov T, Mirč M, Mitrović A, Prokić M, Radovanović T, Gavrilović B, Despotović S, Gavrić J, Petrović T, Tomašević Kolarov N. Desiccation stress response of Bombina variegata tadpoles. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:158.
https://hdl.handle.net/21.15107/rcub_ibiss_5250 .

Kijanović, Ana, Vukov, Tanja, Mirč, Marko, Mitrović, Aleksandar, Prokić, Marko, Radovanović, Tijana, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Petrović, Tamara, Tomašević Kolarov, Nataša, "Desiccation stress response of Bombina variegata tadpoles" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):158,
https://hdl.handle.net/21.15107/rcub_ibiss_5250 .

TY  - CONF
AU  - Mirč, Marko
AU  - Anđelković, Marko
AU  - Urošević, Aleksandar
AU  - Cvijanović, Milena
AU  - Tomašević Kolarov, Nataša
AU  - Ajduković, Maja
AU  - Kijanović, Ana
AU  - Vučić, Tijana
AU  - Vukov, Tanja
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5229
AB  - National parks are, by definition, areas set aside for wildlife protection, but also 
human recreation. Sometimes, the development of tourism-related infrastructure and 
capacities or resource exploitation can impede the primary function of national parks 
– nature conservation. Baseline and trend monitoring are great tools to assess human 
pressure on nature. Amphibian and reptile species have small ranges and are 
vulnerable to changes in their environment which makes them great bioindicators. 
Here we present the results of five-year monitoring activities of amphibian and reptile 
populations at National Park „Kopaonik“, Serbia. Data on batracho- and herpetofauna 
of NP „Kopaonik“ were limited and outdated, so a detailed survey of species richness, 
habitat conditions, and population trends was greatly needed. Starting from 2018 to 
2022 we described the amphibian and reptile diversity of the national park and 
systematically monitored several habitats, dispersed throughout all three protection 
zones as well as outside protection zones, to assess the habitat quality and population 
trends of selected species. We had an opportunity to see the effect of the international 
travel ban (due to the Covid19) on batracho- and herpetofauna, when much more 
people spent their vacation at NP „Kopaonik“ than usually. Per our results so far, the 
richness of the amphibians and reptiles species of the NP „Kopanik“ is moderate (9 
amphibian and 12 reptile species). The population size of the selected lizard species is 
stable through the years regardless of the protection zone. Amphibian populations are 
also stable, as offsprings of the majority of the species were found every year. However 
human pressure on the amphibian and reptile species is high. Habitat degradation is 
present and some localities are at great risk to be lost
PB  - Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade
C3  - Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
T1  - Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia
SP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5229
ER  - 

@conference{
author = "Mirč, Marko and Anđelković, Marko and Urošević, Aleksandar and Cvijanović, Milena and Tomašević Kolarov, Nataša and Ajduković, Maja and Kijanović, Ana and Vučić, Tijana and Vukov, Tanja",
year = "2022",
abstract = "National parks are, by definition, areas set aside for wildlife protection, but also 
human recreation. Sometimes, the development of tourism-related infrastructure and 
capacities or resource exploitation can impede the primary function of national parks 
– nature conservation. Baseline and trend monitoring are great tools to assess human 
pressure on nature. Amphibian and reptile species have small ranges and are 
vulnerable to changes in their environment which makes them great bioindicators. 
Here we present the results of five-year monitoring activities of amphibian and reptile 
populations at National Park „Kopaonik“, Serbia. Data on batracho- and herpetofauna 
of NP „Kopaonik“ were limited and outdated, so a detailed survey of species richness, 
habitat conditions, and population trends was greatly needed. Starting from 2018 to 
2022 we described the amphibian and reptile diversity of the national park and 
systematically monitored several habitats, dispersed throughout all three protection 
zones as well as outside protection zones, to assess the habitat quality and population 
trends of selected species. We had an opportunity to see the effect of the international 
travel ban (due to the Covid19) on batracho- and herpetofauna, when much more 
people spent their vacation at NP „Kopaonik“ than usually. Per our results so far, the 
richness of the amphibians and reptiles species of the NP „Kopanik“ is moderate (9 
amphibian and 12 reptile species). The population size of the selected lizard species is 
stable through the years regardless of the protection zone. Amphibian populations are 
also stable, as offsprings of the majority of the species were found every year. However 
human pressure on the amphibian and reptile species is high. Habitat degradation is 
present and some localities are at great risk to be lost",
publisher = "Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade",
journal = "Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia",
title = "Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia",
pages = "79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5229"
}

Mirč, M., Anđelković, M., Urošević, A., Cvijanović, M., Tomašević Kolarov, N., Ajduković, M., Kijanović, A., Vučić, T.,& Vukov, T.. (2022). Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia
Belgrade: Institute for Biological Research "Siniša Stanković"– National Institute of Republic of Serbia, University of Belgrade., 79.
https://hdl.handle.net/21.15107/rcub_ibiss_5229

Mirč M, Anđelković M, Urošević A, Cvijanović M, Tomašević Kolarov N, Ajduković M, Kijanović A, Vučić T, Vukov T. Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia. in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia. 2022;:79.
https://hdl.handle.net/21.15107/rcub_ibiss_5229 .

Mirč, Marko, Anđelković, Marko, Urošević, Aleksandar, Cvijanović, Milena, Tomašević Kolarov, Nataša, Ajduković, Maja, Kijanović, Ana, Vučić, Tijana, Vukov, Tanja, "Five years of monitoring amphibian and reptile populations at National Park  „Kopaonik“, Serbia" in Program and Book of Abstracts: the 21st European Congress of Herpetology; 2022 Sep 5-9; Belgrade, Serbia (2022):79,
https://hdl.handle.net/21.15107/rcub_ibiss_5229 .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
PY  - 2021
UR  - http://www.ncbi.nlm.nih.gov/pubmed/33220626
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0944200620301239
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4012
AB  - As anuran saltatory locomotion has specific functional requirements achieved through certain intra- and inter-limb proportions, we analyzed pattern and degree of morphological integration in limbs of ten anuran species to reveal the relationship of shared developmental programs of serially homologous structures and locomotor specialization. Our main objectives were (1) to examine if morphological and functional differences in forelimb and hindlimb were associated with reduced covariation between limbs, (2) and to reveal patterns of correlation between species and the roles played by evolutionary history (phylogeny) and ecology (lifestyle and habitat use). Species with different locomotor behaviours (walking, jumping, hopping, running, climbing, swimming and burrowing) were used. Partial correlations showed that species shared similar patterns of functionally based morphological integration, with increased correlations in elements within limbs and reduced correlations between limbs. This was mainly based on strong correlations between proximal elements, humerus-radioulna and femur-tibiofibula. To test the influence of phylogenetic relationships and ecological demands we used different matrices (correlation similarity matrix, ecological similarity matrix, matrices of phylogenetic distance and morphological distance). The changes in correlation patterns are shown to be dissociated from phylogeny. On the other hand, they are to some extent shaped by habitat use and locomotion, as the species with similar locomotor behaviour also tend to have stronger similarity in integration patterns. The results from this study provide insight into the processes underlying the evolutionary change of anuran limbs, highlighting function as the main factor that shaped morphological integration of the examined species.
PB  - Elsevier BV
T2  - Zoology
T1  - Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology
VL  - 144
DO  - 10.1016/j.zool.2020.125864
SP  - 125864
ER  - 

@article{
author = "Petrović, Tamara and Vukov, Tanja and Tomašević Kolarov, Nataša",
year = "2021",
abstract = "As anuran saltatory locomotion has specific functional requirements achieved through certain intra- and inter-limb proportions, we analyzed pattern and degree of morphological integration in limbs of ten anuran species to reveal the relationship of shared developmental programs of serially homologous structures and locomotor specialization. Our main objectives were (1) to examine if morphological and functional differences in forelimb and hindlimb were associated with reduced covariation between limbs, (2) and to reveal patterns of correlation between species and the roles played by evolutionary history (phylogeny) and ecology (lifestyle and habitat use). Species with different locomotor behaviours (walking, jumping, hopping, running, climbing, swimming and burrowing) were used. Partial correlations showed that species shared similar patterns of functionally based morphological integration, with increased correlations in elements within limbs and reduced correlations between limbs. This was mainly based on strong correlations between proximal elements, humerus-radioulna and femur-tibiofibula. To test the influence of phylogenetic relationships and ecological demands we used different matrices (correlation similarity matrix, ecological similarity matrix, matrices of phylogenetic distance and morphological distance). The changes in correlation patterns are shown to be dissociated from phylogeny. On the other hand, they are to some extent shaped by habitat use and locomotion, as the species with similar locomotor behaviour also tend to have stronger similarity in integration patterns. The results from this study provide insight into the processes underlying the evolutionary change of anuran limbs, highlighting function as the main factor that shaped morphological integration of the examined species.",
publisher = "Elsevier BV",
journal = "Zoology",
title = "Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology",
volume = "144",
doi = "10.1016/j.zool.2020.125864",
pages = "125864"
}

Petrović, T., Vukov, T.,& Tomašević Kolarov, N.. (2021). Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology. in Zoology
Elsevier BV., 144, 125864.
https://doi.org/10.1016/j.zool.2020.125864

Petrović T, Vukov T, Tomašević Kolarov N. Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology. in Zoology. 2021;144:125864.
doi:10.1016/j.zool.2020.125864 .

Petrović, Tamara, Vukov, Tanja, Tomašević Kolarov, Nataša, "Patterns of correlations and locomotor specialization in anuran limbs: association with phylogeny and ecology" in Zoology, 144 (2021):125864,
https://doi.org/10.1016/j.zool.2020.125864 . .

TY  - JOUR
AU  - Petrović, Tamara
AU  - Kijanović, Ana
AU  - Tomašević Kolarov, Nataša
AU  - Gavrić, Jelena
AU  - Despotović, Svetlana
AU  - Gavrilović, Branka
AU  - Radovanović, Tijana
AU  - Vukov, Tanja
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://www.mdpi.com/2076-2615/11/4/953
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4192
AB  - In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.
PB  - MDPI AG
T2  - Animals
T1  - Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters
IS  - 4
VL  - 11
DO  - 10.3390/ani11040953
SP  - 953
ER  - 

@article{
author = "Petrović, Tamara and Kijanović, Ana and Tomašević Kolarov, Nataša and Gavrić, Jelena and Despotović, Svetlana and Gavrilović, Branka and Radovanović, Tijana and Vukov, Tanja and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "In this paper, we examined how the oxidative status (antioxidant system and oxidative damage) of Bombina variegata larvae changed during the metamorphic climax (Gosner stages: 42—beginning, 44—middle and 46—end) and compared the patterns and levels of oxidative stress parameters between individuals developing under constant water availability (control) and those developing under decreasing water availability (desiccation group). Our results revealed that larvae developing under decreasing water availability exhibited increased oxidative damage in the middle and end stages. This was followed by lower levels of glutathione in stages 44 and 46, as well as lower values of catalase, glutathione peroxidase, glutathione S-transferase and sulfhydryl groups in stage 46 (all in relation to control animals). Comparison between stages 42, 44 and 46 within treatments showed that individuals in the last stage demonstrated the highest intensities of lipid oxidative damage in both the control and desiccation groups. As for the parameters of the antioxidant system, control individuals displayed greater variety in response to changes induced by metamorphic climax than individuals exposed to desiccation treatment. The overall decrease in water availability during development led to increased oxidative stress and modifications in the pattern of AOS response to changes induced by metamorphic climax in larvae of B. variegata.",
publisher = "MDPI AG",
journal = "Animals",
title = "Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters",
number = "4",
volume = "11",
doi = "10.3390/ani11040953",
pages = "953"
}

Petrović, T., Kijanović, A., Tomašević Kolarov, N., Gavrić, J., Despotović, S., Gavrilović, B., Radovanović, T., Vukov, T., Faggio, C.,& Prokić, M.. (2021). Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters. in Animals
MDPI AG., 11(4), 953.
https://doi.org/10.3390/ani11040953

Petrović T, Kijanović A, Tomašević Kolarov N, Gavrić J, Despotović S, Gavrilović B, Radovanović T, Vukov T, Faggio C, Prokić M. Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters. in Animals. 2021;11(4):953.
doi:10.3390/ani11040953 .

Petrović, Tamara, Kijanović, Ana, Tomašević Kolarov, Nataša, Gavrić, Jelena, Despotović, Svetlana, Gavrilović, Branka, Radovanović, Tijana, Vukov, Tanja, Faggio, Caterina, Prokić, Marko, "Effects of Desiccation on Metamorphic Climax in Bombina variegata: Changes in Levels and Patterns of Oxidative Stress Parameters" in Animals, 11, no. 4 (2021):953,
https://doi.org/10.3390/ani11040953 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621002180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4484
AB  - Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109191
SP  - 109191
ER  - 

@article{
author = "Radovanović, Tijana and Gavrilović, Branka and Petrović, Tamara and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109191",
pages = "109191"
}

Radovanović, T., Gavrilović, B., Petrović, T., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Faggio, C.,& Prokić, M.. (2021). Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 250, 109191.
https://doi.org/10.1016/j.cbpc.2021.109191

Radovanović T, Gavrilović B, Petrović T, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Faggio C, Prokić M. Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109191.
doi:10.1016/j.cbpc.2021.109191 .

Radovanović, Tijana, Gavrilović, Branka, Petrović, Tamara, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Faggio, Caterina, Prokić, Marko, "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109191,
https://doi.org/10.1016/j.cbpc.2021.109191 . .

TY  - JOUR
AU  - Radovanović, Tijana
AU  - Gavrilović, Branka
AU  - Petrović, Tamara
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Faggio, Caterina
AU  - Prokić, Marko
PY  - 2021
UR  - https://linkinghub.elsevier.com/retrieve/pii/S1532045621002180
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4484
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4497
AB  - Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.
PB  - New York: Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
T1  - Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.
VL  - 250
DO  - 10.1016/j.cbpc.2021.109191
SP  - 109191
ER  - 

@article{
author = "Radovanović, Tijana and Gavrilović, Branka and Petrović, Tamara and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Mirč, Marko and Tomašević Kolarov, Nataša and Faggio, Caterina and Prokić, Marko",
year = "2021",
abstract = "Global warming represents a severe threat to existing ecosystems, especially for anuran tadpoles who encounter significant fluctuations in their habitats. Decreasing water levels in permanent and temporary water bodies is a significant risk for larval survival or fitness. On the other hand, the natural environment of amphibians is extremely polluted by various xenobiotics. This study evaluated how pre-exposure of Bombina variegata tadpoles to chronic environmental stress (desiccation) modulates the biochemical response of juvenile individuals to following acute chemical stressor (pesticide deltamethrin). Our results demonstrated that individually applied pesticide changed the thiol and lipid status of the treated juveniles but animals subjected solely to desiccation pressure were more tolerant to free radicals and showed no induction of lipid peroxidation. Comparison of juveniles exposed to deltamethrin revealed that desiccation pretreatment during the larval stage of development modified cellular protection in the juveniles. Higher activities of CAT, GSH-Px and GR were recorded in the pre-exposed group, as well as a lower degree of lipid peroxidation relative to the group that was not pre-exposed to low water stress. Pre-desiccated groups displayed the greatest range of coordination of investigated antioxidant parameters, supported by Pearson's correlations. Activation of the GSH-redox system is a significant marker in juveniles against stress caused by desiccation and a chemical stressor. The stressful environment experienced during tadpole development produced an adaptive reaction to subsequent exposure to another stressor in juveniles. To develop relevant management and conservation strategies, more studies of the interactive effects of environmental and chemical stressors are necessary.",
publisher = "New York: Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology",
title = "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.",
volume = "250",
doi = "10.1016/j.cbpc.2021.109191",
pages = "109191"
}

Radovanović, T., Gavrilović, B., Petrović, T., Despotović, S., Gavrić, J., Kijanović, A., Mirč, M., Tomašević Kolarov, N., Faggio, C.,& Prokić, M.. (2021). Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology
New York: Elsevier Inc.., 250, 109191.
https://doi.org/10.1016/j.cbpc.2021.109191

Radovanović T, Gavrilović B, Petrović T, Despotović S, Gavrić J, Kijanović A, Mirč M, Tomašević Kolarov N, Faggio C, Prokić M. Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles.. in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2021;250:109191.
doi:10.1016/j.cbpc.2021.109191 .

Radovanović, Tijana, Gavrilović, Branka, Petrović, Tamara, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Mirč, Marko, Tomašević Kolarov, Nataša, Faggio, Caterina, Prokić, Marko, "Impact of desiccation pre-exposure on deltamethrin-induced oxidative stress in Bombina variegata juveniles." in Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 250 (2021):109191,
https://doi.org/10.1016/j.cbpc.2021.109191 . .

TY  - JOUR
AU  - Prokić, Marko
AU  - Petrović, Tamara
AU  - Gavrilović, Branka
AU  - Despotović, Svetlana
AU  - Gavrić, Jelena
AU  - Kijanović, Ana
AU  - Tomašević Kolarov, Nataša
AU  - Vukov, Tanja
AU  - Radovanović, Tijana
PY  - 2021
UR  - https://www.frontiersin.org/articles/10.3389/fphys.2021.783288/full
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8674722
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4757
AB  - Amphibians are sensitive to deteriorating environmental conditions, especially during transition to a terrestrial environment which is full of uncertainties. Harsh conditions, such as desiccation during earlier stages, affect different larval traits with possible carry-over effects on juvenile and adult life histories. The first consequences of the effects can be seen in juveniles in the challenges to find food and the ability to survive without it in a terrestrial habitat. Body size and the internal energy reserves acquired during the larval phase play an important role in this period. Herein, we tested how different water regimes (low water availability, desiccation and constant high-water availability) during larval development reflect on the oxidative status and ability of yellow belly toad (Bombina variegata) juveniles to endure short-term fasting. The desiccation regime significantly reduced the body size of metamorphs. The same was observed after 2 weeks of fasting, while the feeding treatment reduced differences mostly in the body mass of individuals from different water regimes. This was the result of a greater gain in mass in juveniles pre-exposed to desiccation. Pre-exposure to desiccation also modified the parameters of the antioxidant system (AOS) under feeding conditions, leading to higher values of superoxide dismutase, glutathione reductase and glutathione S-transferase, glutathione and sulfhydryl group concentrations, and lower glutathione peroxidase in comparison to juveniles reared under constant water. The increase in the AOS of juveniles can be considered as a physiological carry-over effect of desiccation, probably as the result of compensatory growth and/or earlier exposure to chronic stress. However, water levels during larval development did not exert significant effects on the oxidative status of juveniles subjected to food unavailability. Fasting juveniles, both control and desiccated, were exposed to oxidative stress, significantly higher lipid peroxide concentrations, lower superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione and sulfhydryl group values in comparison to feeding individuals. The lack of food in juvenile anurans activated the AOS response in the same manner, regardless of body size and stress pre-exposure, suggesting that the generally accepted hypothesis about the influence of metamorphic body size on the fitness of the postmetamorphic stage should be tested further.
T2  - Frontiers in Physiology
T1  - Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.
VL  - 12
DO  - 10.3389/fphys.2021.783288
SP  - 783288
ER  - 

@article{
author = "Prokić, Marko and Petrović, Tamara and Gavrilović, Branka and Despotović, Svetlana and Gavrić, Jelena and Kijanović, Ana and Tomašević Kolarov, Nataša and Vukov, Tanja and Radovanović, Tijana",
year = "2021",
abstract = "Amphibians are sensitive to deteriorating environmental conditions, especially during transition to a terrestrial environment which is full of uncertainties. Harsh conditions, such as desiccation during earlier stages, affect different larval traits with possible carry-over effects on juvenile and adult life histories. The first consequences of the effects can be seen in juveniles in the challenges to find food and the ability to survive without it in a terrestrial habitat. Body size and the internal energy reserves acquired during the larval phase play an important role in this period. Herein, we tested how different water regimes (low water availability, desiccation and constant high-water availability) during larval development reflect on the oxidative status and ability of yellow belly toad (Bombina variegata) juveniles to endure short-term fasting. The desiccation regime significantly reduced the body size of metamorphs. The same was observed after 2 weeks of fasting, while the feeding treatment reduced differences mostly in the body mass of individuals from different water regimes. This was the result of a greater gain in mass in juveniles pre-exposed to desiccation. Pre-exposure to desiccation also modified the parameters of the antioxidant system (AOS) under feeding conditions, leading to higher values of superoxide dismutase, glutathione reductase and glutathione S-transferase, glutathione and sulfhydryl group concentrations, and lower glutathione peroxidase in comparison to juveniles reared under constant water. The increase in the AOS of juveniles can be considered as a physiological carry-over effect of desiccation, probably as the result of compensatory growth and/or earlier exposure to chronic stress. However, water levels during larval development did not exert significant effects on the oxidative status of juveniles subjected to food unavailability. Fasting juveniles, both control and desiccated, were exposed to oxidative stress, significantly higher lipid peroxide concentrations, lower superoxide dismutase, glutathione peroxidase, glutathione S-transferase, glutathione and sulfhydryl group values in comparison to feeding individuals. The lack of food in juvenile anurans activated the AOS response in the same manner, regardless of body size and stress pre-exposure, suggesting that the generally accepted hypothesis about the influence of metamorphic body size on the fitness of the postmetamorphic stage should be tested further.",
journal = "Frontiers in Physiology",
title = "Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.",
volume = "12",
doi = "10.3389/fphys.2021.783288",
pages = "783288"
}

Prokić, M., Petrović, T., Gavrilović, B., Despotović, S., Gavrić, J., Kijanović, A., Tomašević Kolarov, N., Vukov, T.,& Radovanović, T.. (2021). Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.. in Frontiers in Physiology, 12, 783288.
https://doi.org/10.3389/fphys.2021.783288

Prokić M, Petrović T, Gavrilović B, Despotović S, Gavrić J, Kijanović A, Tomašević Kolarov N, Vukov T, Radovanović T. Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles.. in Frontiers in Physiology. 2021;12:783288.
doi:10.3389/fphys.2021.783288 .

Prokić, Marko, Petrović, Tamara, Gavrilović, Branka, Despotović, Svetlana, Gavrić, Jelena, Kijanović, Ana, Tomašević Kolarov, Nataša, Vukov, Tanja, Radovanović, Tijana, "Carry-Over Effects of Desiccation Stress on the Oxidative Status of Fasting Anuran Juveniles." in Frontiers in Physiology, 12 (2021):783288,
https://doi.org/10.3389/fphys.2021.783288 . .

TY  - JOUR
AU  - Vukov, Tanja
AU  - Mirč, Marko
AU  - Tomašević Kolarov, Nataša
AU  - Stamenković, Srđan
PY  - 2020
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5139
AB  - Numerous studies of urban environment impact on wildlife imply urbanization can have both negative and positive effects. Phenotypic variation of pileus in the common wall lizard (Podarcis muralis) was analysed to determine whether urbanization levels can be associated with developmental instability induced by environmental stress. Pileus developmental pathways and instability in natural, suburban and urban populations were quantified by patterns of size and shape, fluctuating asymmetry (FA), modular organization and integration, allometric trajectories and frequency of phenodeviants. Our results show high asymmetry and modular structure of pileus with the high frequency of phenodeviants for natural, suburban and urban populations indicating elevated developmental instability in all three habitat types. However, some peculiarities were observed comparing habitats – the lowest level of FA and integration in urban populations and unexpectedly high level of FA and frequency of phenodeviants in the natural population. In addition, significant correlations between symmetric and asymmetric shape patterns, and presence of modular organization for all three habitat types suggest that genetic/environmental and developmental parcellation are somewhat aligned. Our results indicate that pileus morphology varies in a complex manner and future studies that link physiological, behavioural and morphological parameters to demographic parameters and fitness are necessary to fully understand how environmental stress affects developmental instability.
PB  - London: Zoological Society of London
T2  - Journal of Zoology
T1  - Urbanization and the common wall lizard (Podarcis muralis) in the Pannonian basin, Serbia: nowhere safe?
IS  - 2
VL  - 310
DO  - doi.org/10.1111/jzo.12735
SP  - 158
EP  - 169
ER  - 

@article{
author = "Vukov, Tanja and Mirč, Marko and Tomašević Kolarov, Nataša and Stamenković, Srđan",
year = "2020",
abstract = "Numerous studies of urban environment impact on wildlife imply urbanization can have both negative and positive effects. Phenotypic variation of pileus in the common wall lizard (Podarcis muralis) was analysed to determine whether urbanization levels can be associated with developmental instability induced by environmental stress. Pileus developmental pathways and instability in natural, suburban and urban populations were quantified by patterns of size and shape, fluctuating asymmetry (FA), modular organization and integration, allometric trajectories and frequency of phenodeviants. Our results show high asymmetry and modular structure of pileus with the high frequency of phenodeviants for natural, suburban and urban populations indicating elevated developmental instability in all three habitat types. However, some peculiarities were observed comparing habitats – the lowest level of FA and integration in urban populations and unexpectedly high level of FA and frequency of phenodeviants in the natural population. In addition, significant correlations between symmetric and asymmetric shape patterns, and presence of modular organization for all three habitat types suggest that genetic/environmental and developmental parcellation are somewhat aligned. Our results indicate that pileus morphology varies in a complex manner and future studies that link physiological, behavioural and morphological parameters to demographic parameters and fitness are necessary to fully understand how environmental stress affects developmental instability.",
publisher = "London: Zoological Society of London",
journal = "Journal of Zoology",
title = "Urbanization and the common wall lizard (Podarcis muralis) in the Pannonian basin, Serbia: nowhere safe?",
number = "2",
volume = "310",
doi = "doi.org/10.1111/jzo.12735",
pages = "158-169"
}

Vukov, T., Mirč, M., Tomašević Kolarov, N.,& Stamenković, S.. (2020). Urbanization and the common wall lizard (Podarcis muralis) in the Pannonian basin, Serbia: nowhere safe?. in Journal of Zoology
London: Zoological Society of London., 310(2), 158-169.
https://doi.org/doi.org/10.1111/jzo.12735

Vukov T, Mirč M, Tomašević Kolarov N, Stamenković S. Urbanization and the common wall lizard (Podarcis muralis) in the Pannonian basin, Serbia: nowhere safe?. in Journal of Zoology. 2020;310(2):158-169.
doi:doi.org/10.1111/jzo.12735 .

Vukov, Tanja, Mirč, Marko, Tomašević Kolarov, Nataša, Stamenković, Srđan, "Urbanization and the common wall lizard (Podarcis muralis) in the Pannonian basin, Serbia: nowhere safe?" in Journal of Zoology, 310, no. 2 (2020):158-169,
https://doi.org/doi.org/10.1111/jzo.12735 . .

TY  - JOUR
AU  - Jovanović, Bogdan
AU  - Kessler, Ethan J.
AU  - Ilić, Marija
AU  - Ćorović, Jelena
AU  - Tomašević Kolarov, Nataša
AU  - Phillips, Christopher A.
AU  - Crnobrnja-Isailović, Jelka
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3585
UR  - https://journals.tubitak.gov.tr/zoology/abstract.htm?id=26272
AB  - We tracked yearly variation in breeding phenology in relation to weather parameters in a common European toad population
from the southeastern part of its range. Phenological data were collected from 2001 to 2003 and from 2011 to 2017 and compared to
open-access daily weather data from a nearby weather station. Data analysis revealed no significant effect of weather on initiation of
breeding and a negative relationship between mean daily humidity and mean cloud cover on the duration of breeding season (P = 0.03).
Further analysis showed a decreasing trend in breeding season humidity in the past 70 years. Our results predict a tendency toward
longer toad breeding seasons in years with drier winter/spring. A projected decrease in humidity in this region could prolong toad
breeding season, potentially exposing adults to higher predation. Therefore, the scenario of further decline in our study population
should be considered and conservation measures planned accordingly.
PB  - Turkiye Klinikleri
T2  - Turkish Journal of Zoology
T1  - Possible implications of weather variation on reproductive phenology of European common toad in southeastern Europe
IS  - 1
VL  - 44
DO  - 10.3906/zoo-1908-49
SP  - 44
EP  - 50
ER  - 

@article{
author = "Jovanović, Bogdan and Kessler, Ethan J. and Ilić, Marija and Ćorović, Jelena and Tomašević Kolarov, Nataša and Phillips, Christopher A. and Crnobrnja-Isailović, Jelka",
year = "2020",
abstract = "We tracked yearly variation in breeding phenology in relation to weather parameters in a common European toad population
from the southeastern part of its range. Phenological data were collected from 2001 to 2003 and from 2011 to 2017 and compared to
open-access daily weather data from a nearby weather station. Data analysis revealed no significant effect of weather on initiation of
breeding and a negative relationship between mean daily humidity and mean cloud cover on the duration of breeding season (P = 0.03).
Further analysis showed a decreasing trend in breeding season humidity in the past 70 years. Our results predict a tendency toward
longer toad breeding seasons in years with drier winter/spring. A projected decrease in humidity in this region could prolong toad
breeding season, potentially exposing adults to higher predation. Therefore, the scenario of further decline in our study population
should be considered and conservation measures planned accordingly.",
publisher = "Turkiye Klinikleri",
journal = "Turkish Journal of Zoology",
title = "Possible implications of weather variation on reproductive phenology of European common toad in southeastern Europe",
number = "1",
volume = "44",
doi = "10.3906/zoo-1908-49",
pages = "44-50"
}

Jovanović, B., Kessler, E. J., Ilić, M., Ćorović, J., Tomašević Kolarov, N., Phillips, C. A.,& Crnobrnja-Isailović, J.. (2020). Possible implications of weather variation on reproductive phenology of European common toad in southeastern Europe. in Turkish Journal of Zoology
Turkiye Klinikleri., 44(1), 44-50.
https://doi.org/10.3906/zoo-1908-49

Jovanović B, Kessler EJ, Ilić M, Ćorović J, Tomašević Kolarov N, Phillips CA, Crnobrnja-Isailović J. Possible implications of weather variation on reproductive phenology of European common toad in southeastern Europe. in Turkish Journal of Zoology. 2020;44(1):44-50.
doi:10.3906/zoo-1908-49 .

Jovanović, Bogdan, Kessler, Ethan J., Ilić, Marija, Ćorović, Jelena, Tomašević Kolarov, Nataša, Phillips, Christopher A., Crnobrnja-Isailović, Jelka, "Possible implications of weather variation on reproductive phenology of European common toad in southeastern Europe" in Turkish Journal of Zoology, 44, no. 1 (2020):44-50,
https://doi.org/10.3906/zoo-1908-49 . .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Sibinović, Maša
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Ivanović, Ana
PY  - 2019
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/evo.13743
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3348
AB  - In vertebrates with complex, biphasic, life cycles, larvae have a distinct morphology and ecological preferences compared to metamorphosed juveniles and adults. In amphibians, abrupt and rapid metamorphic changes transform aquatic larvae to terrestrial juveniles. The main aim of this study is to test whether, relative to larval stages, metamorphosis (1) resets the pattern of variation between ontogenetic stages and species, (2) constrains intraspecific morphological variability, and (3) similar to the "hour-glass" model reduces morphological disparity. We explore postembryonic ontogenetic trajectories of head shape (from hatching to completed metamorphosis) of two well-defined, morphologically distinct Triturus newts species and their F1 hybrids. Variation in head shape is quantified and compared on two levels: dynamic (across ontogenetic stages) and static (at a particular stage). Our results show that the ontogenetic trajectories diverge early during development and continue to diverge throughout larval stages and metamorphosis. The high within-group variance and the largest disparity level (between-group variance) characterize the metamorphosed stage. Hence, our results indicate that metamorphosis does not canalize head shape variation generated during larval development and that metamorphosed phenotype is not more constrained relative to larval ones. Therefore, metamorphosis cannot be regarded as a developmental constraint, at least not for salamander head shape.
T2  - Evolution; International Journal of Organic Evolution
T1  - Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.
DO  - 10.1111/evo.13743
ER  - 

@article{
author = "Vučić, Tijana and Sibinović, Maša and Vukov, Tanja and Tomašević Kolarov, Nataša and Cvijanović, Milena and Ivanović, Ana",
year = "2019",
abstract = "In vertebrates with complex, biphasic, life cycles, larvae have a distinct morphology and ecological preferences compared to metamorphosed juveniles and adults. In amphibians, abrupt and rapid metamorphic changes transform aquatic larvae to terrestrial juveniles. The main aim of this study is to test whether, relative to larval stages, metamorphosis (1) resets the pattern of variation between ontogenetic stages and species, (2) constrains intraspecific morphological variability, and (3) similar to the "hour-glass" model reduces morphological disparity. We explore postembryonic ontogenetic trajectories of head shape (from hatching to completed metamorphosis) of two well-defined, morphologically distinct Triturus newts species and their F1 hybrids. Variation in head shape is quantified and compared on two levels: dynamic (across ontogenetic stages) and static (at a particular stage). Our results show that the ontogenetic trajectories diverge early during development and continue to diverge throughout larval stages and metamorphosis. The high within-group variance and the largest disparity level (between-group variance) characterize the metamorphosed stage. Hence, our results indicate that metamorphosis does not canalize head shape variation generated during larval development and that metamorphosed phenotype is not more constrained relative to larval ones. Therefore, metamorphosis cannot be regarded as a developmental constraint, at least not for salamander head shape.",
journal = "Evolution; International Journal of Organic Evolution",
title = "Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.",
doi = "10.1111/evo.13743"
}

Vučić, T., Sibinović, M., Vukov, T., Tomašević Kolarov, N., Cvijanović, M.,& Ivanović, A.. (2019). Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.. in Evolution; International Journal of Organic Evolution.
https://doi.org/10.1111/evo.13743

Vučić T, Sibinović M, Vukov T, Tomašević Kolarov N, Cvijanović M, Ivanović A. Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts.. in Evolution; International Journal of Organic Evolution. 2019;.
doi:10.1111/evo.13743 .

Vučić, Tijana, Sibinović, Maša, Vukov, Tanja, Tomašević Kolarov, Nataša, Cvijanović, Milena, Ivanović, Ana, "Testing the evolutionary constraints of metamorphosis: The ontogeny of head shape in Triturus newts." in Evolution; International Journal of Organic Evolution (2019),
https://doi.org/10.1111/evo.13743 . .

TY  - CONF
AU  - Kijanović, Ana
AU  - Vukov, Tanja
AU  - Uzum, Nazan
AU  - Olgun, Kurtulus
AU  - Tomašević Kolarov, Nataša
PY  - 2019
UR  - http://seh-congress-2019.unipv.it/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4996
AB  - In recent decades, digit ratio has attracted considerable attention in biology. It has been suggested that the digit ratio may be used as a biomarker of early developmental effects. In particular, the second-to-fourth digit ratio has been linked to the effects of sex hormones and their receptor genes. Also, this ratio is a morphological feature that is important for tetrapod locomotory performances in different microhabitats. Here, we focus on examining sex differences in digit ratios (2D:3D, 2D: 4D and 3D: 4D) in 15 species of the family Salamandridae with different patterns of aquatic to terrestrial locomotion. The main questions of this study are: (1) whether sexual dimorphism exists in digit length and their ratios (2) whether size, phylogeny or ecology has an influence on digit length and its ratios. We assume that different selective pressures related to aquatic and terrestrial locomotion could override
the prenatal developmental cues. The results suggest a different degree of dimorphism in analyzed digits and digits
ratios in analyzed salamanders, with strong effects of ecology, i.e. the proportion of time spent using each
locomotion type, swimming or walking, implying that function has a large impact on limb growth. The results of
our study are in line with others which can help to evaluate whether the developmental or functional processes
have the most impact on limb evolution.
PB  - Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca
C3  - XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
T1  - Sexual dimorphism in digits length and their ratios in Salamander species (Salamandridae)
SP  - 279
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4996
ER  - 

@conference{
author = "Kijanović, Ana and Vukov, Tanja and Uzum, Nazan and Olgun, Kurtulus and Tomašević Kolarov, Nataša",
year = "2019",
abstract = "In recent decades, digit ratio has attracted considerable attention in biology. It has been suggested that the digit ratio may be used as a biomarker of early developmental effects. In particular, the second-to-fourth digit ratio has been linked to the effects of sex hormones and their receptor genes. Also, this ratio is a morphological feature that is important for tetrapod locomotory performances in different microhabitats. Here, we focus on examining sex differences in digit ratios (2D:3D, 2D: 4D and 3D: 4D) in 15 species of the family Salamandridae with different patterns of aquatic to terrestrial locomotion. The main questions of this study are: (1) whether sexual dimorphism exists in digit length and their ratios (2) whether size, phylogeny or ecology has an influence on digit length and its ratios. We assume that different selective pressures related to aquatic and terrestrial locomotion could override
the prenatal developmental cues. The results suggest a different degree of dimorphism in analyzed digits and digits
ratios in analyzed salamanders, with strong effects of ecology, i.e. the proportion of time spent using each
locomotion type, swimming or walking, implying that function has a large impact on limb growth. The results of
our study are in line with others which can help to evaluate whether the developmental or functional processes
have the most impact on limb evolution.",
publisher = "Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca",
journal = "XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy",
title = "Sexual dimorphism in digits length and their ratios in Salamander species (Salamandridae)",
pages = "279",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4996"
}

Kijanović, A., Vukov, T., Uzum, N., Olgun, K.,& Tomašević Kolarov, N.. (2019). Sexual dimorphism in digits length and their ratios in Salamander species (Salamandridae). in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy
Milan, Italy: University of Milan, the University of Pavia, the Natural History Museum of Milan and the University of Milano-Bicocca., 279.
https://hdl.handle.net/21.15107/rcub_ibiss_4996

Kijanović A, Vukov T, Uzum N, Olgun K, Tomašević Kolarov N. Sexual dimorphism in digits length and their ratios in Salamander species (Salamandridae). in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy. 2019;:279.
https://hdl.handle.net/21.15107/rcub_ibiss_4996 .

Kijanović, Ana, Vukov, Tanja, Uzum, Nazan, Olgun, Kurtulus, Tomašević Kolarov, Nataša, "Sexual dimorphism in digits length and their ratios in Salamander species (Salamandridae)" in XX European Congress of Herpetology. Program & Abstracts; 2019 Sep 2-6; Milan, Italy (2019):279,
https://hdl.handle.net/21.15107/rcub_ibiss_4996 .

TY  - JOUR
AU  - Krstičić Račković, Jelena
AU  - Tomašević Kolarov, Nataša
AU  - Labus, Nenad
AU  - Vukov, Tanja
PY  - 2019
UR  - http://link.springer.com/10.1007/s00435-019-00441-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3332
AB  - The vertebrate skull is considered as phylogenetically conserved, but shows extensive diversification in many clades due to various environmental, climate and habitat influences. We explored the cranial size and shape differences of three closely related brown frog species, Rana temporaria, R. dalmatina and R. graeca to emphases the interspecific variation and intraspecific sexual differences, the allometric patterns in cranial shape changes and the relationship between cranial shape, phylogeny, and ecological similarity. Brown frogs significantly differ in cranial size and shape. Interspecific cranial shape changes are under strong influence of size variation. When size is considered, our results show that R. dalmatina is the most divergent species, while differences between R. dalmatina and R. temporaria cranial shapes are diminished when size is removed and peculiar features of R. graeca cranium arise (the most robust and rounded cranium). In both cases, with size and without size, major shape changes are related to the position of quadratum and width of the cranium at the level of anterior margin of eye. When comparing non-allometric shape with ecological similarity and phylogenetic relatedness we found that ecologically similar but phylogenetically more distant species shared the cranial morphology. Intraspecific patterns of cranial phenotypic variation in brown frogs showed significant cranium sexual size and shape dimorphism and absence of allometric scaling between sexes. More detailed studies on the relation of morphology and ecology in brown frogs are necessary to explain the mechanism behind cranial size and shape variation.
T2  - Zoomorphology
T1  - Interspecific size- and sex-related variation in the cranium of European brown frogs (Genus Rana)
DO  - 10.1007/s00435-019-00441-9
ER  - 

@article{
author = "Krstičić Račković, Jelena and Tomašević Kolarov, Nataša and Labus, Nenad and Vukov, Tanja",
year = "2019",
abstract = "The vertebrate skull is considered as phylogenetically conserved, but shows extensive diversification in many clades due to various environmental, climate and habitat influences. We explored the cranial size and shape differences of three closely related brown frog species, Rana temporaria, R. dalmatina and R. graeca to emphases the interspecific variation and intraspecific sexual differences, the allometric patterns in cranial shape changes and the relationship between cranial shape, phylogeny, and ecological similarity. Brown frogs significantly differ in cranial size and shape. Interspecific cranial shape changes are under strong influence of size variation. When size is considered, our results show that R. dalmatina is the most divergent species, while differences between R. dalmatina and R. temporaria cranial shapes are diminished when size is removed and peculiar features of R. graeca cranium arise (the most robust and rounded cranium). In both cases, with size and without size, major shape changes are related to the position of quadratum and width of the cranium at the level of anterior margin of eye. When comparing non-allometric shape with ecological similarity and phylogenetic relatedness we found that ecologically similar but phylogenetically more distant species shared the cranial morphology. Intraspecific patterns of cranial phenotypic variation in brown frogs showed significant cranium sexual size and shape dimorphism and absence of allometric scaling between sexes. More detailed studies on the relation of morphology and ecology in brown frogs are necessary to explain the mechanism behind cranial size and shape variation.",
journal = "Zoomorphology",
title = "Interspecific size- and sex-related variation in the cranium of European brown frogs (Genus Rana)",
doi = "10.1007/s00435-019-00441-9"
}

Krstičić Račković, J., Tomašević Kolarov, N., Labus, N.,& Vukov, T.. (2019). Interspecific size- and sex-related variation in the cranium of European brown frogs (Genus Rana). in Zoomorphology.
https://doi.org/10.1007/s00435-019-00441-9

Krstičić Račković J, Tomašević Kolarov N, Labus N, Vukov T. Interspecific size- and sex-related variation in the cranium of European brown frogs (Genus Rana). in Zoomorphology. 2019;.
doi:10.1007/s00435-019-00441-9 .

Krstičić Račković, Jelena, Tomašević Kolarov, Nataša, Labus, Nenad, Vukov, Tanja, "Interspecific size- and sex-related variation in the cranium of European brown frogs (Genus Rana)" in Zoomorphology (2019),
https://doi.org/10.1007/s00435-019-00441-9 . .

TY  - JOUR
AU  - Mirč, Marko
AU  - Tomašević-Kolarov, Nataša
AU  - Stamenković, Srđan
AU  - Vukov, Tanja
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641900033M
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/4044
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3569
AB  - The use of fluctuating asymmetry (FA) as a measure of developmental instability and its relationship to stress and fitness is highly controversial. We examined whether the selection of different FA indices and traits influences the results of FA analysis. We chose four meristic traits and three FA indices (two single-trait and two multiple-trait indices) to assess FA levels in the common wall lizard (Podarcis muralis) from three different habitat types (urban, suburban and natural). Urbanization has already been linked to developmental instability in P. muralis. We therefore expected to detect different FA levels among the habitats. However, we also wanted to see whether we obtained the same patterns using different indices and traits. Our results showed that different traits can yield different FA patterns between habitats. The only statistically significant difference between habitats was detected for the FA2 index in femoral pores. The highest level of FA was detected in the urban population, while the lowest level was in the natural population. It is clear that caution must be exerted when deciding on which traits and indices are to be used for FA analysis
T2  - Archives of Biological Sciences
T1  - Asymmetry in the common wall lizard Podarcis muralis under different levels of urbanization: The effect of trait and FA index selection
IS  - 3
VL  - 71
DO  - 10.2298/ABS190225033M
SP  - 501
EP  - 508
ER  - 

@article{
author = "Mirč, Marko and Tomašević-Kolarov, Nataša and Stamenković, Srđan and Vukov, Tanja",
year = "2019",
abstract = "The use of fluctuating asymmetry (FA) as a measure of developmental instability and its relationship to stress and fitness is highly controversial. We examined whether the selection of different FA indices and traits influences the results of FA analysis. We chose four meristic traits and three FA indices (two single-trait and two multiple-trait indices) to assess FA levels in the common wall lizard (Podarcis muralis) from three different habitat types (urban, suburban and natural). Urbanization has already been linked to developmental instability in P. muralis. We therefore expected to detect different FA levels among the habitats. However, we also wanted to see whether we obtained the same patterns using different indices and traits. Our results showed that different traits can yield different FA patterns between habitats. The only statistically significant difference between habitats was detected for the FA2 index in femoral pores. The highest level of FA was detected in the urban population, while the lowest level was in the natural population. It is clear that caution must be exerted when deciding on which traits and indices are to be used for FA analysis",
journal = "Archives of Biological Sciences",
title = "Asymmetry in the common wall lizard Podarcis muralis under different levels of urbanization: The effect of trait and FA index selection",
number = "3",
volume = "71",
doi = "10.2298/ABS190225033M",
pages = "501-508"
}

Mirč, M., Tomašević-Kolarov, N., Stamenković, S.,& Vukov, T.. (2019). Asymmetry in the common wall lizard Podarcis muralis under different levels of urbanization: The effect of trait and FA index selection. in Archives of Biological Sciences, 71(3), 501-508.
https://doi.org/10.2298/ABS190225033M

Mirč M, Tomašević-Kolarov N, Stamenković S, Vukov T. Asymmetry in the common wall lizard Podarcis muralis under different levels of urbanization: The effect of trait and FA index selection. in Archives of Biological Sciences. 2019;71(3):501-508.
doi:10.2298/ABS190225033M .

Mirč, Marko, Tomašević-Kolarov, Nataša, Stamenković, Srđan, Vukov, Tanja, "Asymmetry in the common wall lizard Podarcis muralis under different levels of urbanization: The effect of trait and FA index selection" in Archives of Biological Sciences, 71, no. 3 (2019):501-508,
https://doi.org/10.2298/ABS190225033M . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Tomović, Ljiljana
AU  - Krizmanić, Imre
AU  - Anđelković, Marko
AU  - Golubović, Ana
AU  - Maričić, Marko
AU  - Ajtić, Rastko
AU  - Ćorović, Jelena
AU  - Čubrić, Tijana
AU  - Tomašević-Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Vukov, Tanja
AU  - Jovanović, Bogdan
AU  - Vučić, Tijana
AU  - Ajduković, Maja
AU  - Tot, Ivan
AU  - Nadaždin, Bojana
AU  - Labus, Nenad
AU  - Džukić, Georg
PY  - 2018
UR  - http://www.nhmbeo.rs/издања/bulletin.65.html
UR  - http://www.nhmbeo.rs/upload/images/Glasnik/2018_a/Bulletin 2018-09.pdf
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3292
AB  - In this study, we present updated distribution data for all three brown frog species (Rana spp.) inhabiting Serbia. The data provided consists of newly collected field records and compiled data previously published in literature or via Internet. Of the three species found in Serbia, Rana dalmatina is the most widespread, present in all three altitudinal regions and all biogeographical regions. Rana graeca is confined to Mountain-valley altitudinal region in permanent fastflowing rivers or streams and it reaches its northern range boundaries in Serbia. Rana temporaria is the rarest of the three, with fragmented range restricted to the high mountains or few isolated lowland populations, and in the north-eastern Serbia, it is vicariant with R. graeca in canyons and gorges of montane rivers. The presence of Rana arvalis in Serbia is mentioned in literature but was not confirmed during the subsequent field surveys, so we consider it only as a potential species for the Serbian batrachofauna. The brown frog faunal composition of Serbia is identical to that of Albania, Bosnia and Herzegovina, Bulgaria, Greece, Macedonia and Montenegro, while it is somewhat different from that of Hungary and Romania and the most different from Croatia and Slovenia. Serbian brown frogs belong to European (R. temporaria) and South-European (R. dalmatina, R. graeca) chorotypes. All species of brown frogs present or potentially present in Serbia and their habitats are identified as great conservation priorities.
T2  - Bulletin of the Natural History Museum
T1  - Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia
VL  - 11
DO  - 10.5937/bnhmb1306090V
SP  - 227
EP  - 245
ER  - 

@article{
author = "Urošević, Aleksandar and Tomović, Ljiljana and Krizmanić, Imre and Anđelković, Marko and Golubović, Ana and Maričić, Marko and Ajtić, Rastko and Ćorović, Jelena and Čubrić, Tijana and Tomašević-Kolarov, Nataša and Cvijanović, Milena and Vukov, Tanja and Jovanović, Bogdan and Vučić, Tijana and Ajduković, Maja and Tot, Ivan and Nadaždin, Bojana and Labus, Nenad and Džukić, Georg",
year = "2018",
abstract = "In this study, we present updated distribution data for all three brown frog species (Rana spp.) inhabiting Serbia. The data provided consists of newly collected field records and compiled data previously published in literature or via Internet. Of the three species found in Serbia, Rana dalmatina is the most widespread, present in all three altitudinal regions and all biogeographical regions. Rana graeca is confined to Mountain-valley altitudinal region in permanent fastflowing rivers or streams and it reaches its northern range boundaries in Serbia. Rana temporaria is the rarest of the three, with fragmented range restricted to the high mountains or few isolated lowland populations, and in the north-eastern Serbia, it is vicariant with R. graeca in canyons and gorges of montane rivers. The presence of Rana arvalis in Serbia is mentioned in literature but was not confirmed during the subsequent field surveys, so we consider it only as a potential species for the Serbian batrachofauna. The brown frog faunal composition of Serbia is identical to that of Albania, Bosnia and Herzegovina, Bulgaria, Greece, Macedonia and Montenegro, while it is somewhat different from that of Hungary and Romania and the most different from Croatia and Slovenia. Serbian brown frogs belong to European (R. temporaria) and South-European (R. dalmatina, R. graeca) chorotypes. All species of brown frogs present or potentially present in Serbia and their habitats are identified as great conservation priorities.",
journal = "Bulletin of the Natural History Museum",
title = "Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia",
volume = "11",
doi = "10.5937/bnhmb1306090V",
pages = "227-245"
}

Urošević, A., Tomović, L., Krizmanić, I., Anđelković, M., Golubović, A., Maričić, M., Ajtić, R., Ćorović, J., Čubrić, T., Tomašević-Kolarov, N., Cvijanović, M., Vukov, T., Jovanović, B., Vučić, T., Ajduković, M., Tot, I., Nadaždin, B., Labus, N.,& Džukić, G.. (2018). Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia. in Bulletin of the Natural History Museum, 11, 227-245.
https://doi.org/10.5937/bnhmb1306090V

Urošević A, Tomović L, Krizmanić I, Anđelković M, Golubović A, Maričić M, Ajtić R, Ćorović J, Čubrić T, Tomašević-Kolarov N, Cvijanović M, Vukov T, Jovanović B, Vučić T, Ajduković M, Tot I, Nadaždin B, Labus N, Džukić G. Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia. in Bulletin of the Natural History Museum. 2018;11:227-245.
doi:10.5937/bnhmb1306090V .

Urošević, Aleksandar, Tomović, Ljiljana, Krizmanić, Imre, Anđelković, Marko, Golubović, Ana, Maričić, Marko, Ajtić, Rastko, Ćorović, Jelena, Čubrić, Tijana, Tomašević-Kolarov, Nataša, Cvijanović, Milena, Vukov, Tanja, Jovanović, Bogdan, Vučić, Tijana, Ajduković, Maja, Tot, Ivan, Nadaždin, Bojana, Labus, Nenad, Džukić, Georg, "Distribution and diversity of brown frogs (Rana spp., Anura, Amphibia) in Serbia" in Bulletin of the Natural History Museum, 11 (2018):227-245,
https://doi.org/10.5937/bnhmb1306090V . .

TY  - JOUR
AU  - Vučić, Tijana
AU  - Vukov, Tanja
AU  - Tomašević Kolarov, Nataša
AU  - Cvijanović, Milena
AU  - Ivanović, Ana
PY  - 2018
UR  - http://booksandjournals.brillonline.com/content/journals/10.1163/15685381-17000190
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3013
AB  - In amphibians, morphological differentiation and disparity at the larval and post-metamorphic ontogenetic stages can diverge, owing to various contrasting environments and different selective pressures. In the monophyletic clade of nine Triturus newt species, five different morphotypes can be recognized, but information on larval morphology is limited. Here we explore divergence of larval morphology in Triturus ivanbureschi, T. macedonicus, and their F1 hybrids. These two genetically and morphologically distinct crested newt species hybridize in nature and form a relatively wide hybrid zone in the central part of the Balkan Peninsula. Using a geometric morphometric approach and multivariate statistics, we evaluated differences of tail size and shape, colouration pattern, and the presence of a tail filament at the mid-larval stage in larvae reared under controlled laboratory conditions. We chose the tail as the main propulsive organ crucial for locomotion, feeding, and escaping predators. We found that Triturus ivanbureschi and T. macedonicus larvae differ in tail shape, but not in tail size. Two groups of F1 hybrid larvae (obtained from reciprocal crossing) were similar to each other, but differed from the parental species in size and shape of the tail, colouration pattern, and the presence of a tail filament. Our results indicate that, like adults, larvae diverge morphologically and hybrid larvae do not exhibit intermediate morphology of the parental species.
T2  - Amphibia-Reptilia
T1  - The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids
IS  - 1
VL  - 39
DO  - 10.1163/15685381-17000190
SP  - 87
EP  - 97
ER  - 

@article{
author = "Vučić, Tijana and Vukov, Tanja and Tomašević Kolarov, Nataša and Cvijanović, Milena and Ivanović, Ana",
year = "2018",
abstract = "In amphibians, morphological differentiation and disparity at the larval and post-metamorphic ontogenetic stages can diverge, owing to various contrasting environments and different selective pressures. In the monophyletic clade of nine Triturus newt species, five different morphotypes can be recognized, but information on larval morphology is limited. Here we explore divergence of larval morphology in Triturus ivanbureschi, T. macedonicus, and their F1 hybrids. These two genetically and morphologically distinct crested newt species hybridize in nature and form a relatively wide hybrid zone in the central part of the Balkan Peninsula. Using a geometric morphometric approach and multivariate statistics, we evaluated differences of tail size and shape, colouration pattern, and the presence of a tail filament at the mid-larval stage in larvae reared under controlled laboratory conditions. We chose the tail as the main propulsive organ crucial for locomotion, feeding, and escaping predators. We found that Triturus ivanbureschi and T. macedonicus larvae differ in tail shape, but not in tail size. Two groups of F1 hybrid larvae (obtained from reciprocal crossing) were similar to each other, but differed from the parental species in size and shape of the tail, colouration pattern, and the presence of a tail filament. Our results indicate that, like adults, larvae diverge morphologically and hybrid larvae do not exhibit intermediate morphology of the parental species.",
journal = "Amphibia-Reptilia",
title = "The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids",
number = "1",
volume = "39",
doi = "10.1163/15685381-17000190",
pages = "87-97"
}

Vučić, T., Vukov, T., Tomašević Kolarov, N., Cvijanović, M.,& Ivanović, A.. (2018). The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids. in Amphibia-Reptilia, 39(1), 87-97.
https://doi.org/10.1163/15685381-17000190

Vučić T, Vukov T, Tomašević Kolarov N, Cvijanović M, Ivanović A. The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids. in Amphibia-Reptilia. 2018;39(1):87-97.
doi:10.1163/15685381-17000190 .

Vučić, Tijana, Vukov, Tanja, Tomašević Kolarov, Nataša, Cvijanović, Milena, Ivanović, Ana, "The study of larval tail morphology reveals differentiation between two Triturus species and their hybrids" in Amphibia-Reptilia, 39, no. 1 (2018):87-97,
https://doi.org/10.1163/15685381-17000190 . .