TY  - JOUR
AU  - Batinić, Petar
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Cvijetić, Ilija
AU  - Gašić, Uroš
AU  - Čutović, Natalija
AU  - Pešić, Mirjana
AU  - Milinčić, Danijel
AU  - Carević, Tamara
AU  - Marinković, Aleksandar
AU  - Bugarski, Branko
AU  - Marković, Tatjana
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6687
AB  - Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.
PB  - Basel: MDPI
T2  - Pharmaceuticals
T1  - Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia
IS  - 4
VL  - 17
DO  - 10.3390/ph17040518
SP  - 518
ER  - 

@article{
author = "Batinić, Petar and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Cvijetić, Ilija and Gašić, Uroš and Čutović, Natalija and Pešić, Mirjana and Milinčić, Danijel and Carević, Tamara and Marinković, Aleksandar and Bugarski, Branko and Marković, Tatjana",
year = "2024",
abstract = "Without being aware of their chemical composition, many cultures have used herbaceous peony roots for medicinal purposes. Modern phytopreparations intended for use in human therapy require specific knowledge about the chemistry of peony roots and their biological activities. In this study, ethanol–water extracts were prepared by maceration and microwave- and ultrasound-assisted extractions (MAE and UAE, respectively) in order to obtain bioactive molecules from the roots of Paeonia tenuifolia L., Paeonia peregrina Mill., and Paeonia officinalis L. wild growing in Serbia. Chemical characterization; polyphenol and flavonoid content; antioxidant, multianti-enzymatic, and antibacterial activities of extracts; and in vitro gastrointestinal digestion (GID) of hot water extracts were performed. The strongest anti-cholinesterase activity was observed in PT extracts. The highest anti-ABTS (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical potential was observed in PP extracts, whereas against DPPH (2,2-diphenyl-1-picrylhydrazyl radicals), the best results were achieved with PO extracts. Regarding antibacterial activity, extracts were strongly potent against Bacillus cereus. A molecular docking simulation was conducted to gather insights into the binding affinity and interactions of polyphenols and other Paeonia-specific molecules in the active sites of tested enzymes. In vitro GID of Paeonia teas showed a different recovery and behavior of the individual bioactives, with an increased recovery of methyl gallate and digallate and a decreased recovery of paeoniflorin and its derivatives. PT (Gulenovci) and PP (Pirot) extracts obtained by UAE and M were more efficient in the majority of the bioactivity assays. This study represents an initial step toward the possible application of Paeonia root extracts in pharmacy, medicine, and food technologies.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals",
title = "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia",
number = "4",
volume = "17",
doi = "10.3390/ph17040518",
pages = "518"
}

Batinić, P., Jovanović, A., Stojković, D., Zengin, G., Cvijetić, I., Gašić, U., Čutović, N., Pešić, M., Milinčić, D., Carević, T., Marinković, A., Bugarski, B.,& Marković, T.. (2024). Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals
Basel: MDPI., 17(4), 518.
https://doi.org/10.3390/ph17040518

Batinić P, Jovanović A, Stojković D, Zengin G, Cvijetić I, Gašić U, Čutović N, Pešić M, Milinčić D, Carević T, Marinković A, Bugarski B, Marković T. Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia. in Pharmaceuticals. 2024;17(4):518.
doi:10.3390/ph17040518 .

Batinić, Petar, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Cvijetić, Ilija, Gašić, Uroš, Čutović, Natalija, Pešić, Mirjana, Milinčić, Danijel, Carević, Tamara, Marinković, Aleksandar, Bugarski, Branko, Marković, Tatjana, "Phytochemical Analysis, Biological Activities, and Molecular Docking Studies of Root Extracts from Paeonia Species in Serbia" in Pharmaceuticals, 17, no. 4 (2024):518,
https://doi.org/10.3390/ph17040518 . .

TY  - JOUR
AU  - Batinić, Petar
AU  - Čutović, Natalija
AU  - Jovanović, Aleksandra
AU  - Stojković, Dejan
AU  - Zengin, Gokhan
AU  - Carević, Tamara
AU  - Prijić, Željana
AU  - Marinković, Aleksandar
AU  - Marković, Tatjana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6680
AB  - This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.
PB  - Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"
T2  - Lekovite sirovine
T1  - Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia
IS  - 1
VL  - 43
DO  - 10.61652/leksir2343e159B
SP  - e159
ER  - 

@article{
author = "Batinić, Petar and Čutović, Natalija and Jovanović, Aleksandra and Stojković, Dejan and Zengin, Gokhan and Carević, Tamara and Prijić, Željana and Marinković, Aleksandar and Marković, Tatjana",
year = "2023",
abstract = "This study was designed to investigate the impact of different extraction methods (ultrasound-assisted extraction, maceration, and microwave-assisted extraction) on the content of polyphenols and biological activities of leaf extract of Paeonia daurica subsp. daurica collected in Južni Kučaj (Serbia). The total polyphenol and flavonoid content were achieved by the UV-Vis spectroscopy. FTIR analysis was conducted to investigate structural properties of the extracts. In vitro methods (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - ABTS and 2,2-diphenyl-1-picrylhydrazyl – DPPH assays) were carried out to measure the antioxidant activity of the extracts, while their antibacterial activity was tested against three Gram-positive and three Gram-negative bacterial strains. Analyzing the results of antioxidant activity, the highest anti-DPPH potential was achieved in the extracts of LPD obtained by maceration. The extraction method proven to produce the most effective antibacterial agents is maceration. Moreover, inhibitory activity of extracts against acetyl–and butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase was evaluated in the enzyme inhibition assays. Overall, the results of this research indicated the possible use utilization of leaf extracts of Paeonia daurica subsp. daurica Andrews in food technology and pharmacy.",
publisher = "Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"",
journal = "Lekovite sirovine",
title = "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia",
number = "1",
volume = "43",
doi = "10.61652/leksir2343e159B",
pages = "e159"
}

Batinić, P., Čutović, N., Jovanović, A., Stojković, D., Zengin, G., Carević, T., Prijić, Ž., Marinković, A.,& Marković, T.. (2023). Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine
Belgrade: Institute for Medicinal Plant Research "Dr. Josif Pančić"., 43(1), e159.
https://doi.org/10.61652/leksir2343e159B

Batinić P, Čutović N, Jovanović A, Stojković D, Zengin G, Carević T, Prijić Ž, Marinković A, Marković T. Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia. in Lekovite sirovine. 2023;43(1):e159.
doi:10.61652/leksir2343e159B .

Batinić, Petar, Čutović, Natalija, Jovanović, Aleksandra, Stojković, Dejan, Zengin, Gokhan, Carević, Tamara, Prijić, Željana, Marinković, Aleksandar, Marković, Tatjana, "Antioxidant, antibacterial and enzyme inhibitory activity of the leaf extracts of Paeonia daurica Andrews wild growing in Serbia" in Lekovite sirovine, 43, no. 1 (2023):e159,
https://doi.org/10.61652/leksir2343e159B . .

TY  - JOUR
AU  - Marković, Tatjana
AU  - Čutović, Natalija
AU  - Carević, Tamara
AU  - Gašić, Uroš
AU  - Stojković, Dejan
AU  - Xue, Jingqi
AU  - Jovanović, Aleksandra
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6072
AB  - Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat).
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts
IS  - 14
VL  - 24
DO  - 10.3390/ijms241411764
SP  - 11764
ER  - 

@article{
author = "Marković, Tatjana and Čutović, Natalija and Carević, Tamara and Gašić, Uroš and Stojković, Dejan and Xue, Jingqi and Jovanović, Aleksandra",
year = "2023",
abstract = "Paeonia peregrina Mill. is a perennial herbaceous plant species, known for the medicinal value of all of its plant parts, although the chemical composition of the petals is unknown. This study aimed to determine the chemical fingerprint of the petals and also establish the optimal extraction parameters, extraction medium, and extraction method for petals collected from different localities in Serbia. The optimization was performed in order to acquire extracts that are rich in the contents of total polyphenol content (TPC) and total flavonoid content (TFC), and also exhibit strong antioxidant activity. In addition, the influence of the extracts on several human skin pathogens was evaluated, as well as their ability to aid wound closure and act as anti-inflammatory agents. Both the extraction medium and the applied technique significantly influenced the skin-beneficial biological activities, while methanol proved to be a more favorable extraction medium. In conclusion, the extraction conditions that yielded the extract with the richest phenolic content with satisfactory biological potential varied between the assays, while the most promising locality in Serbia for the collection of P. peregrina petals was Pančevo (South Banat).",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts",
number = "14",
volume = "24",
doi = "10.3390/ijms241411764",
pages = "11764"
}

Marković, T., Čutović, N., Carević, T., Gašić, U., Stojković, D., Xue, J.,& Jovanović, A.. (2023). Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts. in International Journal of Molecular Sciences
Basel: MDPI., 24(14), 11764.
https://doi.org/10.3390/ijms241411764

Marković T, Čutović N, Carević T, Gašić U, Stojković D, Xue J, Jovanović A. Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts. in International Journal of Molecular Sciences. 2023;24(14):11764.
doi:10.3390/ijms241411764 .

Marković, Tatjana, Čutović, Natalija, Carević, Tamara, Gašić, Uroš, Stojković, Dejan, Xue, Jingqi, Jovanović, Aleksandra, "Paeonia peregrina Mill Petals as a New Source of Biologically Active Compounds: Chemical Characterization and Skin Regeneration Effects of the Extracts" in International Journal of Molecular Sciences, 24, no. 14 (2023):11764,
https://doi.org/10.3390/ijms241411764 . .

TY  - JOUR
AU  - Jovanović, Aleksandra
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Grdović, Nevena
AU  - Vidaković, Melita
AU  - Mihailović, Mirjana
PY  - 2022
UR  - https://jepm.tfzv.ues.rs.ba/article/190
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6446
AB  - The aim of the present study was the characterization of silymarin and silibinin li-
posomes
via
determination of encapsulation efficiency, particle size, polydispersity
index (PDI), zeta potential, mobility, and conductivity, as well as storage stability
during 28 days at 4ºC and stability after UV irradiation. Encapsulation efficiencies
of silymarin and silibinin were 92.05±1.41% and 87.86±2.06%, respectively. Par-
ticle size and PDI of the liposomes with silymarin were changed from 3541.3±62.5
nm to 2677.0±44.2 nm and from 0.346±0.044 to 0.228±0.036, respectively, dur-
ing the 28-day stability study; particle size and PDI of the liposomes with silibinin
were changed from 2074.7±19.4 nm to 2704.0±35.0 nm and from 0.328±0.030
to 0.456±0.026, respectively. The Zeta potential of the silymarin-loaded liposomes
and silibinin-loaded liposomes was changed from -27.0±0.7 mV to -26.4±0.4 mV
and from -29.4±0.6 mV to -29.0±0.4 mV, respectively. Mobility and conductivity
of the liposomes with silymarin were changed from -2.120±0.057 μmcm/Vs to -
2.067±0.028 μmcm/Vs and from 0.017±0.005 mS/cm to 0.009±0.004 mS/cm,
respectively.  Mobility and conductivity of the liposomal particles with silibinin
were changed from -2.307±0.053 μmcm/Vs to -2.110±0.033 μmcm/Vs and from
0.018±0.003 mS/cm to 0.060±0.001 mS/cm, respectively. UV irradiation did not
affect particle size and PDI of all liposomes, but it caused a decrease in zeta po-
tential: -23.9±0.8 mV for silymarin and -24.5±0.7 mV for silibinin, in mobility: -
1.874±0.064 μmcm/Vs for silymarin and -1.920±0.057 μmcm/Vs for silibinin, and
in conductivity: 0.014±0.001 mS/cm for silymarin and 0.007±0.003 mS/cm for
silibinin. Overall, the obtained results qualify liposomes to be used as silymarin and
silibinin carriers for application in functional foods and pharmaceutical products.
PB  - Faculty of Technology, University of East Sarajevo
T2  - Journal of Engineering and Processing Management
T1  - The characterization of silymarin and silibinin liposomes
IS  - 2
VL  - 14
DO  - 10.7251/JEPM2202040j
SP  - 40
EP  - 45
ER  - 

@article{
author = "Jovanović, Aleksandra and Dinić, Svetlana and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Grdović, Nevena and Vidaković, Melita and Mihailović, Mirjana",
year = "2022",
abstract = "The aim of the present study was the characterization of silymarin and silibinin li-
posomes
via
determination of encapsulation efficiency, particle size, polydispersity
index (PDI), zeta potential, mobility, and conductivity, as well as storage stability
during 28 days at 4ºC and stability after UV irradiation. Encapsulation efficiencies
of silymarin and silibinin were 92.05±1.41% and 87.86±2.06%, respectively. Par-
ticle size and PDI of the liposomes with silymarin were changed from 3541.3±62.5
nm to 2677.0±44.2 nm and from 0.346±0.044 to 0.228±0.036, respectively, dur-
ing the 28-day stability study; particle size and PDI of the liposomes with silibinin
were changed from 2074.7±19.4 nm to 2704.0±35.0 nm and from 0.328±0.030
to 0.456±0.026, respectively. The Zeta potential of the silymarin-loaded liposomes
and silibinin-loaded liposomes was changed from -27.0±0.7 mV to -26.4±0.4 mV
and from -29.4±0.6 mV to -29.0±0.4 mV, respectively. Mobility and conductivity
of the liposomes with silymarin were changed from -2.120±0.057 μmcm/Vs to -
2.067±0.028 μmcm/Vs and from 0.017±0.005 mS/cm to 0.009±0.004 mS/cm,
respectively.  Mobility and conductivity of the liposomal particles with silibinin
were changed from -2.307±0.053 μmcm/Vs to -2.110±0.033 μmcm/Vs and from
0.018±0.003 mS/cm to 0.060±0.001 mS/cm, respectively. UV irradiation did not
affect particle size and PDI of all liposomes, but it caused a decrease in zeta po-
tential: -23.9±0.8 mV for silymarin and -24.5±0.7 mV for silibinin, in mobility: -
1.874±0.064 μmcm/Vs for silymarin and -1.920±0.057 μmcm/Vs for silibinin, and
in conductivity: 0.014±0.001 mS/cm for silymarin and 0.007±0.003 mS/cm for
silibinin. Overall, the obtained results qualify liposomes to be used as silymarin and
silibinin carriers for application in functional foods and pharmaceutical products.",
publisher = "Faculty of Technology, University of East Sarajevo",
journal = "Journal of Engineering and Processing Management",
title = "The characterization of silymarin and silibinin liposomes",
number = "2",
volume = "14",
doi = "10.7251/JEPM2202040j",
pages = "40-45"
}

Jovanović, A., Dinić, S., Uskoković, A., Arambašić Jovanović, J., Grdović, N., Vidaković, M.,& Mihailović, M.. (2022). The characterization of silymarin and silibinin liposomes. in Journal of Engineering and Processing Management
Faculty of Technology, University of East Sarajevo., 14(2), 40-45.
https://doi.org/10.7251/JEPM2202040j

Jovanović A, Dinić S, Uskoković A, Arambašić Jovanović J, Grdović N, Vidaković M, Mihailović M. The characterization of silymarin and silibinin liposomes. in Journal of Engineering and Processing Management. 2022;14(2):40-45.
doi:10.7251/JEPM2202040j .

Jovanović, Aleksandra, Dinić, Svetlana, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Grdović, Nevena, Vidaković, Melita, Mihailović, Mirjana, "The characterization of silymarin and silibinin liposomes" in Journal of Engineering and Processing Management, 14, no. 2 (2022):40-45,
https://doi.org/10.7251/JEPM2202040j . .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Ćujić, Danica
AU  - Gnjatović, Marija
AU  - Stepanović, Sandra
AU  - Dinić, Svetlana
AU  - Mihailović, Mirjana
AU  - Bugarski, Branko
PY  - 2022
UR  - https://icatsconf.org/ICAT22/van
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5256
AB  - In the present research, the storage and UV stability of small unilamellar liposomes (SUVs) with encapsulated
silymarin and silibinin were investigated in terms of changes in vesicle size, polydispersity index (PDI), zeta
potential, mobility, and conductivity. Silymarin and silibinin were encapsulated within phospholipid liposomes
produced using the proliposome method and subsequently sonicated. On the 1st day, vesicle size was 1681.0±55.5
nm for silymarin liposomes and 1884.7±2.5 nm for silibinin liposomes, whereas PDI was 0.321±0.012 and
0.319±0.011. Additionally, the zeta potential of SUVs with silymarin and silibinin was -38.6±0.8 and -37.6±1.2
mV, respectively, whereas mobility and conductivity were -3.03±0.06 μmcm/Vs and 0.149±0.002 mS/cm (for
silymarin sample) and -2.94±0.09 μmcm/Vs and 0.154±0.002 mS/cm (for silibinin sample). After 28 days of storage
at 4°C, there was a statistically significant increase in the vesicle size of SUVs with silymarin and silibinin
(2703.5±44.5 and 2172.5±26.2 nm) and a decrease in zeta potential (-16.7±0.5 and -9.8±0.1 mV), mobility (-
1.31±0.04 and -0.76±0.01 μmcm/Vs), and conductivity (0.027±0.001 and 0.023±0.001 mS/cm). On the other hand,
UV irradiation did not affect changes in vesicle size and PDI of all liposomes, but it caused a decrease in zeta
potential, -32.9±1.0 mV for silymarin and -33.9±0.3 mV for silibinin, mobility, -2.64±0.1 and -2.47±0.2 μmcm/Vs,
and conductivity, 0.131±0.005 and 0.075±0.006 mS/cm. Also, after 28 days of storage, UV-irradiated SUVs with
silymarin and silibinin have significantly higher vesicle size and lower zeta potential, mobility, and conductivity in
comparison to the measurements immediately after UV irradiation. In conclusion, both non-treated and UVirradiated
silymarin- and silibinin-loaded SUVs were unstable during storage at 4°C, resulting in changes in vesicle
size, zeta potential, mobility, and conductivity, thus additional experiments for improving liposomal stability should
be performed.
PB  - Çumra, Turkey: Plusbase Akademi Publishing
C3  - Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey
T1  - Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin
SP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5256
ER  - 

@conference{
author = "Jovanović, Aleksandra and Ćujić, Danica and Gnjatović, Marija and Stepanović, Sandra and Dinić, Svetlana and Mihailović, Mirjana and Bugarski, Branko",
year = "2022",
abstract = "In the present research, the storage and UV stability of small unilamellar liposomes (SUVs) with encapsulated
silymarin and silibinin were investigated in terms of changes in vesicle size, polydispersity index (PDI), zeta
potential, mobility, and conductivity. Silymarin and silibinin were encapsulated within phospholipid liposomes
produced using the proliposome method and subsequently sonicated. On the 1st day, vesicle size was 1681.0±55.5
nm for silymarin liposomes and 1884.7±2.5 nm for silibinin liposomes, whereas PDI was 0.321±0.012 and
0.319±0.011. Additionally, the zeta potential of SUVs with silymarin and silibinin was -38.6±0.8 and -37.6±1.2
mV, respectively, whereas mobility and conductivity were -3.03±0.06 μmcm/Vs and 0.149±0.002 mS/cm (for
silymarin sample) and -2.94±0.09 μmcm/Vs and 0.154±0.002 mS/cm (for silibinin sample). After 28 days of storage
at 4°C, there was a statistically significant increase in the vesicle size of SUVs with silymarin and silibinin
(2703.5±44.5 and 2172.5±26.2 nm) and a decrease in zeta potential (-16.7±0.5 and -9.8±0.1 mV), mobility (-
1.31±0.04 and -0.76±0.01 μmcm/Vs), and conductivity (0.027±0.001 and 0.023±0.001 mS/cm). On the other hand,
UV irradiation did not affect changes in vesicle size and PDI of all liposomes, but it caused a decrease in zeta
potential, -32.9±1.0 mV for silymarin and -33.9±0.3 mV for silibinin, mobility, -2.64±0.1 and -2.47±0.2 μmcm/Vs,
and conductivity, 0.131±0.005 and 0.075±0.006 mS/cm. Also, after 28 days of storage, UV-irradiated SUVs with
silymarin and silibinin have significantly higher vesicle size and lower zeta potential, mobility, and conductivity in
comparison to the measurements immediately after UV irradiation. In conclusion, both non-treated and UVirradiated
silymarin- and silibinin-loaded SUVs were unstable during storage at 4°C, resulting in changes in vesicle
size, zeta potential, mobility, and conductivity, thus additional experiments for improving liposomal stability should
be performed.",
publisher = "Çumra, Turkey: Plusbase Akademi Publishing",
journal = "Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey",
title = "Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin",
pages = "158",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5256"
}

Jovanović, A., Ćujić, D., Gnjatović, M., Stepanović, S., Dinić, S., Mihailović, M.,& Bugarski, B.. (2022). Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin. in Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey
Çumra, Turkey: Plusbase Akademi Publishing., 158.
https://hdl.handle.net/21.15107/rcub_ibiss_5256

Jovanović A, Ćujić D, Gnjatović M, Stepanović S, Dinić S, Mihailović M, Bugarski B. Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin. in Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey. 2022;:158.
https://hdl.handle.net/21.15107/rcub_ibiss_5256 .

Jovanović, Aleksandra, Ćujić, Danica, Gnjatović, Marija, Stepanović, Sandra, Dinić, Svetlana, Mihailović, Mirjana, Bugarski, Branko, "Storage and UV stability of small unilamellar liposomes with encapsulated silymarin and silibinin" in Abstract Book: 10th International Conference on Advanced Technologies: ICAT'22; 2022 Nov 25-27; Van, Turkey (2022):158,
https://hdl.handle.net/21.15107/rcub_ibiss_5256 .

TY  - CONF
AU  - Jovanović, Aleksandra
AU  - Dinić, Svetlana
AU  - Uskoković, Aleksandra
AU  - Arambašić Jovanović, Jelena
AU  - Grdović, Nevena
AU  - Vidaković, Melita
AU  - Mihailović, Mirjana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5255
AB  - The aim of the present study was the characterization of silymarin and silibinin loaded liposomes via determination of encapsulation efficiency, particle size, polydispersity index (PDI), zeta potential, mobility, and conductivity, as well as storage stability during 28 days at 4ºC and stability after UV irradiation. Encapsulation efficiency of silymarin and silibinin were 92.05±1.41% and 87.86±2.06%, respectively. Particle size and PDI of the liposomes with silymarin were changed from 3541.3±62.5 nm to 2677.0±44.2 nm and from 0.346±0.044 to 0.228±0.036, respectively, during the 28-days stability study; particle size and PDI of the liposomes with silibinin were changed from 2074.7±19.4 nm to 2704.0±35.0 nm and from 0.328±0.030 to 0.456±0.026, respectively. Zeta potential of the silymarin-liposomes and silibinin-liposomes was changed from -27.0±0.7 mV to -26.4±0.4 mV and from -29.4±0.6 mV to -29.0±0.4 mV, respectively. Mobility and conductivity of the liposomes with silymarin were changed from -2.120±0.057 µmcm/Vs to -2.067±0.028 µmcm/Vs and from 0.017±0.005 mS/cm to 0.009±0.004 mS/cm, respectively. Mobility and conductivity of the liposomal particles with silibinin were changed from -2.307±0.053 µmcm/Vs to -2.110±0.033 µmcm/Vs and from 0.018±0.003 mS/cm to 0.060±0.001 mS/cm, respectively. UV irradiation did not affect particle size and PDI of all liposomes, but it caused a decrease in zeta potential: -23.9±0.8 mV for silymarin and -24.5±0.7 mV for silibinin, in mobility: -1.874±0.064 µmcm/Vs for silymarin and -1.920±0.057 µmcm/Vs for silibinin, and in conductivity: 0.014±0.001 mS/cm for silymarin and 0.007±0.003 mS/cm for silibinin. Overall, the obtained results qualify liposomes to be used as silymarin and silibinin carriers for application in functional foods and pharmaceutical products.
PB  - Banja Luka: University of Banja Luka
C3  - XIV conference of chemists, technologists and environmentalists of Republic of Srpska; 2022 Oct 21-2; Banja Luka, Bosnia and Herzegovinia
T1  - The characterisation of silymarin and silibinin loaded liposomes
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5255
ER  - 

@conference{
editor = "Malinović, Borislav",
author = "Jovanović, Aleksandra and Dinić, Svetlana and Uskoković, Aleksandra and Arambašić Jovanović, Jelena and Grdović, Nevena and Vidaković, Melita and Mihailović, Mirjana",
year = "2022",
abstract = "The aim of the present study was the characterization of silymarin and silibinin loaded liposomes via determination of encapsulation efficiency, particle size, polydispersity index (PDI), zeta potential, mobility, and conductivity, as well as storage stability during 28 days at 4ºC and stability after UV irradiation. Encapsulation efficiency of silymarin and silibinin were 92.05±1.41% and 87.86±2.06%, respectively. Particle size and PDI of the liposomes with silymarin were changed from 3541.3±62.5 nm to 2677.0±44.2 nm and from 0.346±0.044 to 0.228±0.036, respectively, during the 28-days stability study; particle size and PDI of the liposomes with silibinin were changed from 2074.7±19.4 nm to 2704.0±35.0 nm and from 0.328±0.030 to 0.456±0.026, respectively. Zeta potential of the silymarin-liposomes and silibinin-liposomes was changed from -27.0±0.7 mV to -26.4±0.4 mV and from -29.4±0.6 mV to -29.0±0.4 mV, respectively. Mobility and conductivity of the liposomes with silymarin were changed from -2.120±0.057 µmcm/Vs to -2.067±0.028 µmcm/Vs and from 0.017±0.005 mS/cm to 0.009±0.004 mS/cm, respectively. Mobility and conductivity of the liposomal particles with silibinin were changed from -2.307±0.053 µmcm/Vs to -2.110±0.033 µmcm/Vs and from 0.018±0.003 mS/cm to 0.060±0.001 mS/cm, respectively. UV irradiation did not affect particle size and PDI of all liposomes, but it caused a decrease in zeta potential: -23.9±0.8 mV for silymarin and -24.5±0.7 mV for silibinin, in mobility: -1.874±0.064 µmcm/Vs for silymarin and -1.920±0.057 µmcm/Vs for silibinin, and in conductivity: 0.014±0.001 mS/cm for silymarin and 0.007±0.003 mS/cm for silibinin. Overall, the obtained results qualify liposomes to be used as silymarin and silibinin carriers for application in functional foods and pharmaceutical products.",
publisher = "Banja Luka: University of Banja Luka",
journal = "XIV conference of chemists, technologists and environmentalists of Republic of Srpska; 2022 Oct 21-2; Banja Luka, Bosnia and Herzegovinia",
title = "The characterisation of silymarin and silibinin loaded liposomes",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5255"
}

Malinović, B., Jovanović, A., Dinić, S., Uskoković, A., Arambašić Jovanović, J., Grdović, N., Vidaković, M.,& Mihailović, M.. (2022). The characterisation of silymarin and silibinin loaded liposomes. in XIV conference of chemists, technologists and environmentalists of Republic of Srpska; 2022 Oct 21-2; Banja Luka, Bosnia and Herzegovinia
Banja Luka: University of Banja Luka..
https://hdl.handle.net/21.15107/rcub_ibiss_5255

Malinović B, Jovanović A, Dinić S, Uskoković A, Arambašić Jovanović J, Grdović N, Vidaković M, Mihailović M. The characterisation of silymarin and silibinin loaded liposomes. in XIV conference of chemists, technologists and environmentalists of Republic of Srpska; 2022 Oct 21-2; Banja Luka, Bosnia and Herzegovinia. 2022;.
https://hdl.handle.net/21.15107/rcub_ibiss_5255 .

Malinović, Borislav, Jovanović, Aleksandra, Dinić, Svetlana, Uskoković, Aleksandra, Arambašić Jovanović, Jelena, Grdović, Nevena, Vidaković, Melita, Mihailović, Mirjana, "The characterisation of silymarin and silibinin loaded liposomes" in XIV conference of chemists, technologists and environmentalists of Republic of Srpska; 2022 Oct 21-2; Banja Luka, Bosnia and Herzegovinia (2022),
https://hdl.handle.net/21.15107/rcub_ibiss_5255 .