TY  - JOUR
AU  - Stanković, Jelena
AU  - Milošević, Đurađ
AU  - Paunović, Momir
AU  - Jovanović, Boris
AU  - Popović, Nataša
AU  - Tomović, Jelena
AU  - Atanacković, Ana
AU  - Radulović, Katarina
AU  - Lončarević, Davor
AU  - Raković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6635
AB  - This study was carried out at the Danube River and its tributaries during the Joint Danube
Survey 4 (JDS4) expedition. Three freshwater benthic species were used to estimate the quantity
of microplastics (MPs): Corbicula spp., Limnodrilus hoffmeisteri (Claparede, 1862), and Polypedilum
nubeculosum (Meigen, 1804). Following the kick and sweep technique, individuals were sampled using
a hand net or dredge. In order to estimate the number of MP particles/individual particles/g wet
body mass, the body mass and total length of all specimens were measured. Alkaline (Corbicula spp.
and L. hoffmaisteri) and enzymatic (P. nubeculosum) protocols were performed for tissue degradation.
All samples were filtered through glass microfiber filters (mesh size 0.5 μm). The particles were
photographed, measured, and counted. A total of 1904, 169, and 204 MPs were isolated from
Corbicula spp., L. hoffmaisteri, and P. nubeculosum, respectively. To confirm the chemical composition
of isolated MPs, a subsample of 46 particles of the fragmented particles from 14 sampling sites was
analysed via μ-ATR-FTIR spectroscopy analysis. The particles were characterised as polycarbonate
(PC), polyethylene terephthalate (PET), polypropylene–polyethylene copolymer (PP-PE), nylon
(polyamide-PA) and cellophane, with the domination of PET.
PB  - Basel: MDPI
T2  - Water
T1  - Microplastics in the Danube River and Its Main Tributaries—Ingestion by Freshwater Macroinvertebrates
IS  - 7
VL  - 16
DO  - 10.3390/w16070962
SP  - 962
ER  - 

@article{
author = "Stanković, Jelena and Milošević, Đurađ and Paunović, Momir and Jovanović, Boris and Popović, Nataša and Tomović, Jelena and Atanacković, Ana and Radulović, Katarina and Lončarević, Davor and Raković, Maja",
year = "2024",
abstract = "This study was carried out at the Danube River and its tributaries during the Joint Danube
Survey 4 (JDS4) expedition. Three freshwater benthic species were used to estimate the quantity
of microplastics (MPs): Corbicula spp., Limnodrilus hoffmeisteri (Claparede, 1862), and Polypedilum
nubeculosum (Meigen, 1804). Following the kick and sweep technique, individuals were sampled using
a hand net or dredge. In order to estimate the number of MP particles/individual particles/g wet
body mass, the body mass and total length of all specimens were measured. Alkaline (Corbicula spp.
and L. hoffmaisteri) and enzymatic (P. nubeculosum) protocols were performed for tissue degradation.
All samples were filtered through glass microfiber filters (mesh size 0.5 μm). The particles were
photographed, measured, and counted. A total of 1904, 169, and 204 MPs were isolated from
Corbicula spp., L. hoffmaisteri, and P. nubeculosum, respectively. To confirm the chemical composition
of isolated MPs, a subsample of 46 particles of the fragmented particles from 14 sampling sites was
analysed via μ-ATR-FTIR spectroscopy analysis. The particles were characterised as polycarbonate
(PC), polyethylene terephthalate (PET), polypropylene–polyethylene copolymer (PP-PE), nylon
(polyamide-PA) and cellophane, with the domination of PET.",
publisher = "Basel: MDPI",
journal = "Water",
title = "Microplastics in the Danube River and Its Main Tributaries—Ingestion by Freshwater Macroinvertebrates",
number = "7",
volume = "16",
doi = "10.3390/w16070962",
pages = "962"
}

Stanković, J., Milošević, Đ., Paunović, M., Jovanović, B., Popović, N., Tomović, J., Atanacković, A., Radulović, K., Lončarević, D.,& Raković, M.. (2024). Microplastics in the Danube River and Its Main Tributaries—Ingestion by Freshwater Macroinvertebrates. in Water
Basel: MDPI., 16(7), 962.
https://doi.org/10.3390/w16070962

Stanković J, Milošević Đ, Paunović M, Jovanović B, Popović N, Tomović J, Atanacković A, Radulović K, Lončarević D, Raković M. Microplastics in the Danube River and Its Main Tributaries—Ingestion by Freshwater Macroinvertebrates. in Water. 2024;16(7):962.
doi:10.3390/w16070962 .

Stanković, Jelena, Milošević, Đurađ, Paunović, Momir, Jovanović, Boris, Popović, Nataša, Tomović, Jelena, Atanacković, Ana, Radulović, Katarina, Lončarević, Davor, Raković, Maja, "Microplastics in the Danube River and Its Main Tributaries—Ingestion by Freshwater Macroinvertebrates" in Water, 16, no. 7 (2024):962,
https://doi.org/10.3390/w16070962 . .

TY  - CONF
AU  - Stanković, Jelena
AU  - Paunović, Momir
AU  - Milošević, Đurađ
AU  - Lončarević, Davor
AU  - Radulović, Katarina
AU  - Kolarević, Stoimir
AU  - Tomović, Jelena
AU  - Raković, Maja
PY  - 2023
UR  - https://www.oen-iad.org/congress2023/documents/conference%20book_44th_iad%20conference_2023.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6478
AB  - During the Joint Danube Survey 4 in 2019, we investigated plastic debris in Danube River, from Germany to the Black Sea (more than 2,000 km of the river stretch). The main aim of the study was to categorize and to quantify microplastic particles in living systems. Freshwater Asian clams Corbicula fluminea (Müller, 1774) was used as test organism. Individuals were collected from 23 sites using a hand net (ap. 25 cm× 25 cm, mesh size 500 μm). In order to isolate plastic particles, the samples were digested by alkaline method, using a KOH 10% solution and incubation at 65 ⁰C for 12 h. The digested samples were filtrated through a glass microfiber filters, with 0.5 µm mesh size. Collected particles were photographed and categorized based on the size and coloration. Particles were counted manually, photographed using Nikon SMZ 745T Stereomicroscope and measured in program ImageJ. In 216 examined specimens a total of 1,998 microplastic particles were isolated with an average of 5.59 ± 3.71 fibrils and 4.37 ± 2.46 fragments per organism; or 40.77 ± 73.75 fibrils and 25.84 ± 33.17 fragments per g body weight. Dominant microplastic particles were between 0.4 and 0.5 mm in diameter (characterized as medium-sized), with an average length 0.43 mm ± 0.26 in the Danube and 0.49 mm ± 0.26 in the tributaries. In order to confirm chemical composition of isolated microliter, 46 particles of the hard plastic from 14 sampling sites were analyzed using Nicolet iN10 Fourier transform infrared microscope with micro ATR accessory and cooled MCT detector, using 128 scans at resolution of 4 cm1 . Analyses revealed presence of five different types of polymers, with the domination of polyethylene-terephthalate.
PB  - Austrian Committee - International Association for Danube Research
C3  - Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
T1  - Isolation of microplastics from freshwater Asian clams Corbicula fluminea (Müller, 1774) in the Danube River
SP  - 21
EP  - 21
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6478
ER  - 

@conference{
author = "Stanković, Jelena and Paunović, Momir and Milošević, Đurađ and Lončarević, Davor and Radulović, Katarina and Kolarević, Stoimir and Tomović, Jelena and Raković, Maja",
year = "2023",
abstract = "During the Joint Danube Survey 4 in 2019, we investigated plastic debris in Danube River, from Germany to the Black Sea (more than 2,000 km of the river stretch). The main aim of the study was to categorize and to quantify microplastic particles in living systems. Freshwater Asian clams Corbicula fluminea (Müller, 1774) was used as test organism. Individuals were collected from 23 sites using a hand net (ap. 25 cm× 25 cm, mesh size 500 μm). In order to isolate plastic particles, the samples were digested by alkaline method, using a KOH 10% solution and incubation at 65 ⁰C for 12 h. The digested samples were filtrated through a glass microfiber filters, with 0.5 µm mesh size. Collected particles were photographed and categorized based on the size and coloration. Particles were counted manually, photographed using Nikon SMZ 745T Stereomicroscope and measured in program ImageJ. In 216 examined specimens a total of 1,998 microplastic particles were isolated with an average of 5.59 ± 3.71 fibrils and 4.37 ± 2.46 fragments per organism; or 40.77 ± 73.75 fibrils and 25.84 ± 33.17 fragments per g body weight. Dominant microplastic particles were between 0.4 and 0.5 mm in diameter (characterized as medium-sized), with an average length 0.43 mm ± 0.26 in the Danube and 0.49 mm ± 0.26 in the tributaries. In order to confirm chemical composition of isolated microliter, 46 particles of the hard plastic from 14 sampling sites were analyzed using Nicolet iN10 Fourier transform infrared microscope with micro ATR accessory and cooled MCT detector, using 128 scans at resolution of 4 cm1 . Analyses revealed presence of five different types of polymers, with the domination of polyethylene-terephthalate.",
publisher = "Austrian Committee - International Association for Danube Research",
journal = "Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria",
title = "Isolation of microplastics from freshwater Asian clams Corbicula fluminea (Müller, 1774) in the Danube River",
pages = "21-21",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6478"
}

Stanković, J., Paunović, M., Milošević, Đ., Lončarević, D., Radulović, K., Kolarević, S., Tomović, J.,& Raković, M.. (2023). Isolation of microplastics from freshwater Asian clams Corbicula fluminea (Müller, 1774) in the Danube River. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria
Austrian Committee - International Association for Danube Research., 21-21.
https://hdl.handle.net/21.15107/rcub_ibiss_6478

Stanković J, Paunović M, Milošević Đ, Lončarević D, Radulović K, Kolarević S, Tomović J, Raković M. Isolation of microplastics from freshwater Asian clams Corbicula fluminea (Müller, 1774) in the Danube River. in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria. 2023;:21-21.
https://hdl.handle.net/21.15107/rcub_ibiss_6478 .

Stanković, Jelena, Paunović, Momir, Milošević, Đurađ, Lončarević, Davor, Radulović, Katarina, Kolarević, Stoimir, Tomović, Jelena, Raković, Maja, "Isolation of microplastics from freshwater Asian clams Corbicula fluminea (Müller, 1774) in the Danube River" in Conference Book: 44th IAD conference: Tackling Present & Future Environmental Challenges of a European Riverscape; 2023 Feb 6-9; Krems, Austria (2023):21-21,
https://hdl.handle.net/21.15107/rcub_ibiss_6478 .