TY  - JOUR
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6647
AB  - The invasion of non-native plant species has a detrimental effect on native biodiversity. In a seven-year research project, we investigated the occurrence of the invasive species Asclepias syriaca L.in the Deliblato Sands protected area, located at a south-eastern part of the Pannonian Plain, and identified the factors that contribute significantly to its colonisation. The distribution of this invasive species was monitored on more than 300 km of the accessory, bordering and selected internal roads. A. syriaca occurs within the protected area but is much more widespread on accessory and bordering roads. The number of locations of A. syriaca increased every year of the study, even within the protected area, indicating a further spread of this species. A. syriaca is much more abundant on the north-eastern edge than in the south-west. The reason for this is most likely the much larger area of abandoned agricultural land in the north-east. Roads used for public transport are the main entry points for the further spread of A. syriaca. In contrast, recreational trail use does not enhance the spread in the Deliblato Sands natural reserve. This study can be used to further analyse the ecological dynamics of A. syriaca and to develop timely strategies by which to prevent or slow down its spread.
PB  - Basel: MDPI
T2  - Forests
T1  - Multi-year monitoring of Asclepias syriaca L. spread in the Deliblato sands protected reserve in Serbia
IS  - 2
VL  - 15
DO  - 10.3390/f15020347
SP  - 347
ER  - 

@article{
author = "Avramov, Stevan and Miljković, Danijela and Barišić Klisarić, Nataša and Živković, Uroš and Tarasjev, Aleksej",
year = "2024",
abstract = "The invasion of non-native plant species has a detrimental effect on native biodiversity. In a seven-year research project, we investigated the occurrence of the invasive species Asclepias syriaca L.in the Deliblato Sands protected area, located at a south-eastern part of the Pannonian Plain, and identified the factors that contribute significantly to its colonisation. The distribution of this invasive species was monitored on more than 300 km of the accessory, bordering and selected internal roads. A. syriaca occurs within the protected area but is much more widespread on accessory and bordering roads. The number of locations of A. syriaca increased every year of the study, even within the protected area, indicating a further spread of this species. A. syriaca is much more abundant on the north-eastern edge than in the south-west. The reason for this is most likely the much larger area of abandoned agricultural land in the north-east. Roads used for public transport are the main entry points for the further spread of A. syriaca. In contrast, recreational trail use does not enhance the spread in the Deliblato Sands natural reserve. This study can be used to further analyse the ecological dynamics of A. syriaca and to develop timely strategies by which to prevent or slow down its spread.",
publisher = "Basel: MDPI",
journal = "Forests",
title = "Multi-year monitoring of Asclepias syriaca L. spread in the Deliblato sands protected reserve in Serbia",
number = "2",
volume = "15",
doi = "10.3390/f15020347",
pages = "347"
}

Avramov, S., Miljković, D., Barišić Klisarić, N., Živković, U.,& Tarasjev, A.. (2024). Multi-year monitoring of Asclepias syriaca L. spread in the Deliblato sands protected reserve in Serbia. in Forests
Basel: MDPI., 15(2), 347.
https://doi.org/10.3390/f15020347

Avramov S, Miljković D, Barišić Klisarić N, Živković U, Tarasjev A. Multi-year monitoring of Asclepias syriaca L. spread in the Deliblato sands protected reserve in Serbia. in Forests. 2024;15(2):347.
doi:10.3390/f15020347 .

Avramov, Stevan, Miljković, Danijela, Barišić Klisarić, Nataša, Živković, Uroš, Tarasjev, Aleksej, "Multi-year monitoring of Asclepias syriaca L. spread in the Deliblato sands protected reserve in Serbia" in Forests, 15, no. 2 (2024):347,
https://doi.org/10.3390/f15020347 . .

TY  - CONF
AU  - Avramov, Stevan
AU  - Banjanac, Tijana
AU  - Brkušanin, Miloš
AU  - Šiler, Branislav
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6441
AB  - Utilization of microsatellite molecular markers was analyzed in Iris pumila and five other species 
belonging to the same genus: I. humilis, I. sibirica, I. pseudacorus, I. spuria and I. variegata, which 
inhabit the northern part of the Republic of Serbia. We selected 40 EST-SSR markers previously 
reported for the genus Iris. Sixteen markers that showed polymorphism were chosen for further 
research. To examine the potential of the selected microsatellite loci analysis in the detection of 
different I. pumila clones, we selected 18 individuals of this species that belonged to different 
genotypes. Optimization of amplification was performed for all 16 microsatellite loci: temperature 
program of the PCR protocol and number of amplification cycles were optimized as well as primer 
and DNA concentrations used in the reactions. Seven markers that were variable across the ana lyzed individuals were selected. The final analysis was performed using only 5 microsatellite loci 
since high-quality amplification for two loci was not possible. No greater deviations in the length 
of the fragments from the expected were observed, except for one primer. All microsatellite loci 
contained three-nucleotide repeats. I. pumila is tetraploid species and in several plants the presence of four alleles were observed. The observed number of alleles per locus ranged from 3 to 9. 
In the results obtained across 18 I. pumila individuals using 5 microsatellite markers, 17 multilocus 
genotypes were observed.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Analysis of amplification of microsatellite loci in Iris pumila
SP  - 147
EP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6441
ER  - 

@conference{
author = "Avramov, Stevan and Banjanac, Tijana and Brkušanin, Miloš and Šiler, Branislav and Miljković, Danijela and Barišić Klisarić, Nataša and Živković, Uroš and Tarasjev, Aleksej",
year = "2022",
abstract = "Utilization of microsatellite molecular markers was analyzed in Iris pumila and five other species 
belonging to the same genus: I. humilis, I. sibirica, I. pseudacorus, I. spuria and I. variegata, which 
inhabit the northern part of the Republic of Serbia. We selected 40 EST-SSR markers previously 
reported for the genus Iris. Sixteen markers that showed polymorphism were chosen for further 
research. To examine the potential of the selected microsatellite loci analysis in the detection of 
different I. pumila clones, we selected 18 individuals of this species that belonged to different 
genotypes. Optimization of amplification was performed for all 16 microsatellite loci: temperature 
program of the PCR protocol and number of amplification cycles were optimized as well as primer 
and DNA concentrations used in the reactions. Seven markers that were variable across the ana lyzed individuals were selected. The final analysis was performed using only 5 microsatellite loci 
since high-quality amplification for two loci was not possible. No greater deviations in the length 
of the fragments from the expected were observed, except for one primer. All microsatellite loci 
contained three-nucleotide repeats. I. pumila is tetraploid species and in several plants the presence of four alleles were observed. The observed number of alleles per locus ranged from 3 to 9. 
In the results obtained across 18 I. pumila individuals using 5 microsatellite markers, 17 multilocus 
genotypes were observed.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Analysis of amplification of microsatellite loci in Iris pumila",
pages = "147-147",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6441"
}

Avramov, S., Banjanac, T., Brkušanin, M., Šiler, B., Miljković, D., Barišić Klisarić, N., Živković, U.,& Tarasjev, A.. (2022). Analysis of amplification of microsatellite loci in Iris pumila. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6441

Avramov S, Banjanac T, Brkušanin M, Šiler B, Miljković D, Barišić Klisarić N, Živković U, Tarasjev A. Analysis of amplification of microsatellite loci in Iris pumila. in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:147-147.
https://hdl.handle.net/21.15107/rcub_ibiss_6441 .

Avramov, Stevan, Banjanac, Tijana, Brkušanin, Miloš, Šiler, Branislav, Miljković, Danijela, Barišić Klisarić, Nataša, Živković, Uroš, Tarasjev, Aleksej, "Analysis of amplification of microsatellite loci in Iris pumila" in Book of Abstracts: 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):147-147,
https://hdl.handle.net/21.15107/rcub_ibiss_6441 .

TY  - CONF
AU  - Tarasjev, Aleksej
AU  - Barišić Klisarić, Nataša
AU  - Avramov, Stevan
AU  - Živković, Uroš
AU  - Miljković, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6673
AB  - In 2012 we selected 35 experimental plots in Iris pumila L. (dwarf bearded iris) natural population
in natural reserve Deliblato Sand, Serbia. Flowering was monitored in 2013, 2014 and 2015
on selected more than 4 000 square meters, and opening of every individual flower on I. pumila
clones was recorded. Data revealed steady decline in number of flowering individual plants (ramets)
through analyzed years. The same spots were revisited in 2021 and 2022 flowering seasons
and occurence of all flowers was recorded by introducing drone digital photography in our
long term study. Preliminary analysis on the subsample of monitored spots reveals that detected
trend of decline in number of flowering ramets was not reversed after ten years. Detected long
term trend has significant implications for studies of evolutionary relationship between vegetative
and sexual modes of reproduction in this species, as well as possible conservation management
strategies in the face of environmental change. Additional methods that can reveal evolutionary
consequences of observed population dynamics on maintenance of within population genetic
polymorphism (with special emphasis on flower color polymorphism and variability on DNA level)
are discussed. We propose application of field spectography to huge flower color polymorphism
observed in analyzed species coupled with pigment HPLC analysis on studied population level,
as well as microsatellite genetic analyses of studied population composition as most suitable and
efficient lines of further research of those topics in the studied populations.
PB  - Beograd: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Flowering trends in Iris pumila in Deliblato Sand: ten years after
SP  - 141
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6673
ER  - 

@conference{
author = "Tarasjev, Aleksej and Barišić Klisarić, Nataša and Avramov, Stevan and Živković, Uroš and Miljković, Danijela",
year = "2022",
abstract = "In 2012 we selected 35 experimental plots in Iris pumila L. (dwarf bearded iris) natural population
in natural reserve Deliblato Sand, Serbia. Flowering was monitored in 2013, 2014 and 2015
on selected more than 4 000 square meters, and opening of every individual flower on I. pumila
clones was recorded. Data revealed steady decline in number of flowering individual plants (ramets)
through analyzed years. The same spots were revisited in 2021 and 2022 flowering seasons
and occurence of all flowers was recorded by introducing drone digital photography in our
long term study. Preliminary analysis on the subsample of monitored spots reveals that detected
trend of decline in number of flowering ramets was not reversed after ten years. Detected long
term trend has significant implications for studies of evolutionary relationship between vegetative
and sexual modes of reproduction in this species, as well as possible conservation management
strategies in the face of environmental change. Additional methods that can reveal evolutionary
consequences of observed population dynamics on maintenance of within population genetic
polymorphism (with special emphasis on flower color polymorphism and variability on DNA level)
are discussed. We propose application of field spectography to huge flower color polymorphism
observed in analyzed species coupled with pigment HPLC analysis on studied population level,
as well as microsatellite genetic analyses of studied population composition as most suitable and
efficient lines of further research of those topics in the studied populations.",
publisher = "Beograd: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Flowering trends in Iris pumila in Deliblato Sand: ten years after",
pages = "141-141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6673"
}

Tarasjev, A., Barišić Klisarić, N., Avramov, S., Živković, U.,& Miljković, D.. (2022). Flowering trends in Iris pumila in Deliblato Sand: ten years after. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Beograd: Serbian Plant Physiology Society., 141-141.
https://hdl.handle.net/21.15107/rcub_ibiss_6673

Tarasjev A, Barišić Klisarić N, Avramov S, Živković U, Miljković D. Flowering trends in Iris pumila in Deliblato Sand: ten years after. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:141-141.
https://hdl.handle.net/21.15107/rcub_ibiss_6673 .

Tarasjev, Aleksej, Barišić Klisarić, Nataša, Avramov, Stevan, Živković, Uroš, Miljković, Danijela, "Flowering trends in Iris pumila in Deliblato Sand: ten years after" in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):141-141,
https://hdl.handle.net/21.15107/rcub_ibiss_6673 .

TY  - CONF
AU  - Miljković, Danijela
AU  - Živković, Uroš
AU  - Barišić Klisarić, Nataša
AU  - Avramov, Stevan
AU  - Tarasjev, Aleksej
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6672
AB  - The development sensitivities of flower parts of Iris variegata were studied in experimental conditions
with changes in light intensity as stressor. As index of environmental stress and developmental
instability we used indices of fluctuating asymmetry (FA8 as size-corrected index and FA17
as composite index of all traits measured on standard and fall flower parts). The 103 genotypes
from three native habitats (open, shaded and semi-shaded) were examined in two light intensities
(low and high). The measurements have been taken at five places along the longitudinal axis of
the flower parts in relation to the width from the main nerve to the edge of the object. Larger
values of the size-corrected index FA8 were found in the low light treatment for genotypes originating
from the open, while for genotypes originating from the shaded habitat development instability
was greater in the high light intensity treatment. Genotypes from semi-shaded habitat did
not show significant responses in both fluctuating assymetry indices to changes in light intensity.
The composite index FA17 showed the opposite pattern for genotypes from the shaded habitat
with higher values in the low light treatment, while genotypes from open habitat showed no
significant response. These results illustrated that light intensity may be an important factor contributing
to bilateral fluctuating asymmetry of flower parts when environment of genotype origin
is taken into consideration, and depending on the choosen index of developmental instability.
PB  - Beograd: Serbian Plant Physiology Society
C3  - Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
T1  - Changes in light intensity induce the developmental instability of Iris variegata flower parts
SP  - 140
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6672
ER  - 

@conference{
author = "Miljković, Danijela and Živković, Uroš and Barišić Klisarić, Nataša and Avramov, Stevan and Tarasjev, Aleksej",
year = "2022",
abstract = "The development sensitivities of flower parts of Iris variegata were studied in experimental conditions
with changes in light intensity as stressor. As index of environmental stress and developmental
instability we used indices of fluctuating asymmetry (FA8 as size-corrected index and FA17
as composite index of all traits measured on standard and fall flower parts). The 103 genotypes
from three native habitats (open, shaded and semi-shaded) were examined in two light intensities
(low and high). The measurements have been taken at five places along the longitudinal axis of
the flower parts in relation to the width from the main nerve to the edge of the object. Larger
values of the size-corrected index FA8 were found in the low light treatment for genotypes originating
from the open, while for genotypes originating from the shaded habitat development instability
was greater in the high light intensity treatment. Genotypes from semi-shaded habitat did
not show significant responses in both fluctuating assymetry indices to changes in light intensity.
The composite index FA17 showed the opposite pattern for genotypes from the shaded habitat
with higher values in the low light treatment, while genotypes from open habitat showed no
significant response. These results illustrated that light intensity may be an important factor contributing
to bilateral fluctuating asymmetry of flower parts when environment of genotype origin
is taken into consideration, and depending on the choosen index of developmental instability.",
publisher = "Beograd: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia",
title = "Changes in light intensity induce the developmental instability of Iris variegata flower parts",
pages = "140-140",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6672"
}

Miljković, D., Živković, U., Barišić Klisarić, N., Avramov, S.,& Tarasjev, A.. (2022). Changes in light intensity induce the developmental instability of Iris variegata flower parts. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia
Beograd: Serbian Plant Physiology Society., 140-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6672

Miljković D, Živković U, Barišić Klisarić N, Avramov S, Tarasjev A. Changes in light intensity induce the developmental instability of Iris variegata flower parts. in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia. 2022;:140-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6672 .

Miljković, Danijela, Živković, Uroš, Barišić Klisarić, Nataša, Avramov, Stevan, Tarasjev, Aleksej, "Changes in light intensity induce the developmental instability of Iris variegata flower parts" in Book of Abstracts: 4th International Conference on Plant Biology and 23rd SPPS Meeting; 2022 6-8; Belgrade, Serbia (2022):140-140,
https://hdl.handle.net/21.15107/rcub_ibiss_6672 .

TY  - CONF
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6671
AB  - Asclepias syriaca, (common milkweed), is an invasive species that has already been naturalized in 23 countries worldwide. Anticipated climate changes are predicted to help further its future spread beyond its current distribution. The common milkweed occurs in various habitats, most often those modified by humans, such as roadsides, railway areas, wastelands, abandoned orchards, vineyards, and abandoned arable land. Its massive occurrence threatens native species diversity, penetrating into natural and seminatural habitats. Deliblato Sands, special Serbian nature reserve, the largest European
continental sandy terrain located in the south-east part of the Pannonia Plain, is also subject to invasion by A.syriaca. In 2015 we analyzed the presence of A.syriaca on borders of natural protected reserve along the roads leading to reserve, as well as on approx. 200 km of roads and paths in the natural reserve itselve. Those inner roads and paths were subsequently monitored in 2017, 2019, and 2021. for persistence and spread of already detected locations of A.syriaca and the occurrence of the new ones. Influence of proximity of reserve borders with different levels of infestation, type of use (commercial
roads, tourist tracking paths), and levels of protection (three levels of protection exist on D. Sand), were analyzed. The largest increase in numbers was detected in the areas with the most intensive human activity. This study of dispersal and range extension can serve as a basis for further analysis of eco-evolutionary dynamics on A.syriaca populations and localities in the Deliblato Sand natural reserve.
PB  - Molenstraat: European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
T1  - Multi-year monitoring of Asclepias syriaca spread in protected reserve of Deliblato Sand in Serbia
SP  - 549
EP  - 549
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6671
ER  - 

@conference{
author = "Avramov, Stevan and Miljković, Danijela and Barišić Klisarić, Nataša and Živković, Uroš and Tarasjev, Aleksej",
year = "2022",
abstract = "Asclepias syriaca, (common milkweed), is an invasive species that has already been naturalized in 23 countries worldwide. Anticipated climate changes are predicted to help further its future spread beyond its current distribution. The common milkweed occurs in various habitats, most often those modified by humans, such as roadsides, railway areas, wastelands, abandoned orchards, vineyards, and abandoned arable land. Its massive occurrence threatens native species diversity, penetrating into natural and seminatural habitats. Deliblato Sands, special Serbian nature reserve, the largest European
continental sandy terrain located in the south-east part of the Pannonia Plain, is also subject to invasion by A.syriaca. In 2015 we analyzed the presence of A.syriaca on borders of natural protected reserve along the roads leading to reserve, as well as on approx. 200 km of roads and paths in the natural reserve itselve. Those inner roads and paths were subsequently monitored in 2017, 2019, and 2021. for persistence and spread of already detected locations of A.syriaca and the occurrence of the new ones. Influence of proximity of reserve borders with different levels of infestation, type of use (commercial
roads, tourist tracking paths), and levels of protection (three levels of protection exist on D. Sand), were analyzed. The largest increase in numbers was detected in the areas with the most intensive human activity. This study of dispersal and range extension can serve as a basis for further analysis of eco-evolutionary dynamics on A.syriaca populations and localities in the Deliblato Sand natural reserve.",
publisher = "Molenstraat: European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic",
title = "Multi-year monitoring of Asclepias syriaca spread in protected reserve of Deliblato Sand in Serbia",
pages = "549-549",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6671"
}

Avramov, S., Miljković, D., Barišić Klisarić, N., Živković, U.,& Tarasjev, A.. (2022). Multi-year monitoring of Asclepias syriaca spread in protected reserve of Deliblato Sand in Serbia. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
Molenstraat: European Society for Evolutionary Biology., 549-549.
https://hdl.handle.net/21.15107/rcub_ibiss_6671

Avramov S, Miljković D, Barišić Klisarić N, Živković U, Tarasjev A. Multi-year monitoring of Asclepias syriaca spread in protected reserve of Deliblato Sand in Serbia. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic. 2022;:549-549.
https://hdl.handle.net/21.15107/rcub_ibiss_6671 .

Avramov, Stevan, Miljković, Danijela, Barišić Klisarić, Nataša, Živković, Uroš, Tarasjev, Aleksej, "Multi-year monitoring of Asclepias syriaca spread in protected reserve of Deliblato Sand in Serbia" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic (2022):549-549,
https://hdl.handle.net/21.15107/rcub_ibiss_6671 .

TY  - CONF
AU  - Barišić Klisarić, Nataša
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2022
UR  - https://www.eseb2022.cz/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5330
AB  - Huge flower color polymorphism found in natural populations of Iris pumila, endangered
species inhabiting the Natural reserve of Deliblato Sand, Serbia, enables the
identification of genetically distinct clones and makes this species a superb object for
genetic polymorphism studies. Evolutionary biology studies of the dwarf bearded iris
were conducted and published by the researchers from our department in the last four
decades with special emphasis on evolutionary ecology and ecological genetics.
However, these studies had several important limitations. Subjectivity in visual
identification led to inconsistencies in defining color morphs. In our current research,
variability in flower color is precisely accessed by reflectance UV-enhanced spectroscopy
in the field, removing subjectivity in visual identification and influence of surrounding light
conditions. Those specters reveal both discrete and continuous variation and will be
followed by pigment HPLC analysis providing insights into the biochemical basis of
flower color polymorphism. Limitations of previous studies also included smaller samples
restricted to the flowering peak. Therefore, we are now also conducting a long-term study
of the dynamics of color-morph frequencies using digital photography (including ones
taken by drone), involving several complete flowering seasons on selected spots
(covering over 4000 square meters and including tens of thousands of flowers) in
undisturbed I.pumila natural population. This approach could reveal how precisely
determined color-morph frequencies change both temporally and spatially in their natural
habitat in the face of changing environments and vegetative succession and in
perspective the role of natural selection vs. random events in maintaining flower-color
polymorphism.
PB  - European Society for Evolutionary Biology
C3  - Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
T1  - Huge flower color polymorphism in Iris pumila L: previous studies and current research
SP  - 509
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5330
ER  - 

@conference{
author = "Barišić Klisarić, Nataša and Avramov, Stevan and Miljković, Danijela and Živković, Uroš and Tarasjev, Aleksej",
year = "2022",
abstract = "Huge flower color polymorphism found in natural populations of Iris pumila, endangered
species inhabiting the Natural reserve of Deliblato Sand, Serbia, enables the
identification of genetically distinct clones and makes this species a superb object for
genetic polymorphism studies. Evolutionary biology studies of the dwarf bearded iris
were conducted and published by the researchers from our department in the last four
decades with special emphasis on evolutionary ecology and ecological genetics.
However, these studies had several important limitations. Subjectivity in visual
identification led to inconsistencies in defining color morphs. In our current research,
variability in flower color is precisely accessed by reflectance UV-enhanced spectroscopy
in the field, removing subjectivity in visual identification and influence of surrounding light
conditions. Those specters reveal both discrete and continuous variation and will be
followed by pigment HPLC analysis providing insights into the biochemical basis of
flower color polymorphism. Limitations of previous studies also included smaller samples
restricted to the flowering peak. Therefore, we are now also conducting a long-term study
of the dynamics of color-morph frequencies using digital photography (including ones
taken by drone), involving several complete flowering seasons on selected spots
(covering over 4000 square meters and including tens of thousands of flowers) in
undisturbed I.pumila natural population. This approach could reveal how precisely
determined color-morph frequencies change both temporally and spatially in their natural
habitat in the face of changing environments and vegetative succession and in
perspective the role of natural selection vs. random events in maintaining flower-color
polymorphism.",
publisher = "European Society for Evolutionary Biology",
journal = "Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic",
title = "Huge flower color polymorphism in Iris pumila L: previous studies and current research",
pages = "509",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5330"
}

Barišić Klisarić, N., Avramov, S., Miljković, D., Živković, U.,& Tarasjev, A.. (2022). Huge flower color polymorphism in Iris pumila L: previous studies and current research. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic
European Society for Evolutionary Biology., 509.
https://hdl.handle.net/21.15107/rcub_ibiss_5330

Barišić Klisarić N, Avramov S, Miljković D, Živković U, Tarasjev A. Huge flower color polymorphism in Iris pumila L: previous studies and current research. in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic. 2022;:509.
https://hdl.handle.net/21.15107/rcub_ibiss_5330 .

Barišić Klisarić, Nataša, Avramov, Stevan, Miljković, Danijela, Živković, Uroš, Tarasjev, Aleksej, "Huge flower color polymorphism in Iris pumila L: previous studies and current research" in Book of Abstracts: Congress of the European Society for Evolutionary Biology; 2022 Aug 14-19; Prague, Czech Republic (2022):509,
https://hdl.handle.net/21.15107/rcub_ibiss_5330 .

TY  - JOUR
AU  - Živković, Uroš
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Tubić, Ljiljana
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Tarasjev, Aleksej
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/8/1599
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC8401273
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4456
AB  - A plant's main mechanism to diminish the effects caused by high free radical levels generated during high irradiance is the synthesis of various secondary metabolites. In addition to interspecies differences, their concentrations may be influenced by genetic, ontogenic, morphogenetic or environmental factors. We investigated the influence of genetic (genotypes from different natural habitats) and environmental (contrasting light regimes as well as successive parts of the vegetation period) variability on the accumulation of 10 selected phenolic compounds (phenolic acids, flavonoids, and xanthones) in Iris variegata genotypes. Genotypes originated from either sun-exposed or shaded natural habitats were transplanted to two experimental light treatments (high light intensity with a higher R/FR ratio and low light intensity with a lower R/FR ratio). Significant impacts of both genetic and environmental seasonal variability (spring, summer and fall during the vegetation period) on phenolic compound profiles were detected. Their highest amounts were detected in spring. The magnitude of difference between light treatments (high vs. low light intensity) and the direction of this change varied depending on the secondary compound class. Phenotypic correlations among the 10 analyzed secondary metabolites differed across the experimental light treatments and their number decreased from spring to fall.
PB  - Basel: MDPI
T2  - Plants (Basel, Switzerland)
T1  - Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L.
IS  - 8
VL  - 10
DO  - 10.3390/plants10081599
SP  - 1599
ER  - 

@article{
author = "Živković, Uroš and Avramov, Stevan and Miljković, Danijela and Barišić Klisarić, Nataša and Tubić, Ljiljana and Mišić, Danijela and Šiler, Branislav and Tarasjev, Aleksej",
year = "2021",
abstract = "A plant's main mechanism to diminish the effects caused by high free radical levels generated during high irradiance is the synthesis of various secondary metabolites. In addition to interspecies differences, their concentrations may be influenced by genetic, ontogenic, morphogenetic or environmental factors. We investigated the influence of genetic (genotypes from different natural habitats) and environmental (contrasting light regimes as well as successive parts of the vegetation period) variability on the accumulation of 10 selected phenolic compounds (phenolic acids, flavonoids, and xanthones) in Iris variegata genotypes. Genotypes originated from either sun-exposed or shaded natural habitats were transplanted to two experimental light treatments (high light intensity with a higher R/FR ratio and low light intensity with a lower R/FR ratio). Significant impacts of both genetic and environmental seasonal variability (spring, summer and fall during the vegetation period) on phenolic compound profiles were detected. Their highest amounts were detected in spring. The magnitude of difference between light treatments (high vs. low light intensity) and the direction of this change varied depending on the secondary compound class. Phenotypic correlations among the 10 analyzed secondary metabolites differed across the experimental light treatments and their number decreased from spring to fall.",
publisher = "Basel: MDPI",
journal = "Plants (Basel, Switzerland)",
title = "Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L.",
number = "8",
volume = "10",
doi = "10.3390/plants10081599",
pages = "1599"
}

Živković, U., Avramov, S., Miljković, D., Barišić Klisarić, N., Tubić, L., Mišić, D., Šiler, B.,& Tarasjev, A.. (2021). Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L.. in Plants (Basel, Switzerland)
Basel: MDPI., 10(8), 1599.
https://doi.org/10.3390/plants10081599

Živković U, Avramov S, Miljković D, Barišić Klisarić N, Tubić L, Mišić D, Šiler B, Tarasjev A. Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L.. in Plants (Basel, Switzerland). 2021;10(8):1599.
doi:10.3390/plants10081599 .

Živković, Uroš, Avramov, Stevan, Miljković, Danijela, Barišić Klisarić, Nataša, Tubić, Ljiljana, Mišić, Danijela, Šiler, Branislav, Tarasjev, Aleksej, "Genetic and Environmental Factors Jointly Impact Leaf Phenolic Profiles of Iris variegata L." in Plants (Basel, Switzerland), 10, no. 8 (2021):1599,
https://doi.org/10.3390/plants10081599 . .

TY  - JOUR
AU  - Barišić Klisarić, Nataša
AU  - Miljković, Danijela
AU  - Avramov, Stevan
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642100032B
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6596
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4650
AB  - Phenotypic correlations were calculated and the levels of phenotypic integration of three congeneric species, annual Lamium purpureum and perennial Lamium album and Lamium maculatum, were estimated. Calculations were performed separately for different stages of development during transplantation under different density and light treatments. L. purpureum exhibited greater integration in comparison to the other two species before exposure to environmental treatments, as well as greater variability in phenotypic correlations in response to light and density. The perennial species possessed similar correlation patterns in comparison to annual L. purpureum, becoming more similar to L. purpureum at later stages of development. The effects of density and light intensity were dependent on the specific combination of factors, as well as on the species under study. Density effects in L. purpureum were also habitat- and population-specific. A greater difference in response to density treatments between habitats than between populations was detected. Overall, phenotypes become more integrated during ontogeny, while dissimilarities in phenotypic correlations among species and between populations were more related to ecological than to phylogenetic differences.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Phenotypic integration in three Lamium species’ response to light and density
IS  - 3
VL  - 73
DO  - 10.2298/ABS210512032B
SP  - 389
EP  - 398
ER  - 

@article{
author = "Barišić Klisarić, Nataša and Miljković, Danijela and Avramov, Stevan and Živković, Uroš and Tarasjev, Aleksej",
year = "2021",
abstract = "Phenotypic correlations were calculated and the levels of phenotypic integration of three congeneric species, annual Lamium purpureum and perennial Lamium album and Lamium maculatum, were estimated. Calculations were performed separately for different stages of development during transplantation under different density and light treatments. L. purpureum exhibited greater integration in comparison to the other two species before exposure to environmental treatments, as well as greater variability in phenotypic correlations in response to light and density. The perennial species possessed similar correlation patterns in comparison to annual L. purpureum, becoming more similar to L. purpureum at later stages of development. The effects of density and light intensity were dependent on the specific combination of factors, as well as on the species under study. Density effects in L. purpureum were also habitat- and population-specific. A greater difference in response to density treatments between habitats than between populations was detected. Overall, phenotypes become more integrated during ontogeny, while dissimilarities in phenotypic correlations among species and between populations were more related to ecological than to phylogenetic differences.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Phenotypic integration in three Lamium species’ response to light and density",
number = "3",
volume = "73",
doi = "10.2298/ABS210512032B",
pages = "389-398"
}

Barišić Klisarić, N., Miljković, D., Avramov, S., Živković, U.,& Tarasjev, A.. (2021). Phenotypic integration in three Lamium species’ response to light and density. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 73(3), 389-398.
https://doi.org/10.2298/ABS210512032B

Barišić Klisarić N, Miljković D, Avramov S, Živković U, Tarasjev A. Phenotypic integration in three Lamium species’ response to light and density. in Archives of Biological Sciences. 2021;73(3):389-398.
doi:10.2298/ABS210512032B .

Barišić Klisarić, Nataša, Miljković, Danijela, Avramov, Stevan, Živković, Uroš, Tarasjev, Aleksej, "Phenotypic integration in three Lamium species’ response to light and density" in Archives of Biological Sciences, 73, no. 3 (2021):389-398,
https://doi.org/10.2298/ABS210512032B . .

TY  - CONF
AU  - Barišić Klisarić, Nataša
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2020
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5450
AB  - Higher plants have developed protection mechanisms in order to diminish the
effects caused by the presence of free radicals generated during high
irradiance, and one of the detoxification mechanisms is the synthesis of
secondary metabolites. In this study we investigated the amount and pattern
of individual phenotypic responses (represented through targeted phenolics
content in 68 genotypes of Iris variegata) to seasonal changes in
environmental conditions under two experimental light regimes. The goal was
to examine how environmental stressors mold the interrelationships between
metabolite traits. Genotypes of I. variegata were collected in Deliblato sands
Special Nature Reserve in Serbia, from two types of natural habitats (exposed
and shade) and transferred into the experimental field conditions providing
either 1. high light intensity and higher red / far red light ratio and 2. low light
intensity and lower red / far red light ratio. After period of acclimatization
leaves were collected during spring, summer and fall of one experimental year
and subjected to UHPLC/qqqMS quantification of 10 selected phenolic
compounds belonging to the groups of phenolic acids, flavonoids and
xanthones. The correlations between all studied compounds within two light
treatments and three seasons were in almost all cases significant and positive.
Number of statistically significant correlations markedly decreased through
the vegetative period in both light treatments. The experiment was conducted
on a large number of genotypes of I. variegata and points to the need of
involving a number of factors in future ecological and evolutionary researches.
PB  - Banja Luka: Faculty of Natural Sciences and Mathematics, University of Banja Luka
C3  - IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020: Book of Abstracts; 2020 Nov 12-14; Banja Luka, Bosnia and Herzegovinia
T1  - Correlation patterns in three Lamium species grown in two light and three density treatments
SP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5450
ER  - 

@conference{
author = "Barišić Klisarić, Nataša and Avramov, Stevan and Miljković, Danijela and Živković, Uroš and Tarasjev, Aleksej",
year = "2020",
abstract = "Higher plants have developed protection mechanisms in order to diminish the
effects caused by the presence of free radicals generated during high
irradiance, and one of the detoxification mechanisms is the synthesis of
secondary metabolites. In this study we investigated the amount and pattern
of individual phenotypic responses (represented through targeted phenolics
content in 68 genotypes of Iris variegata) to seasonal changes in
environmental conditions under two experimental light regimes. The goal was
to examine how environmental stressors mold the interrelationships between
metabolite traits. Genotypes of I. variegata were collected in Deliblato sands
Special Nature Reserve in Serbia, from two types of natural habitats (exposed
and shade) and transferred into the experimental field conditions providing
either 1. high light intensity and higher red / far red light ratio and 2. low light
intensity and lower red / far red light ratio. After period of acclimatization
leaves were collected during spring, summer and fall of one experimental year
and subjected to UHPLC/qqqMS quantification of 10 selected phenolic
compounds belonging to the groups of phenolic acids, flavonoids and
xanthones. The correlations between all studied compounds within two light
treatments and three seasons were in almost all cases significant and positive.
Number of statistically significant correlations markedly decreased through
the vegetative period in both light treatments. The experiment was conducted
on a large number of genotypes of I. variegata and points to the need of
involving a number of factors in future ecological and evolutionary researches.",
publisher = "Banja Luka: Faculty of Natural Sciences and Mathematics, University of Banja Luka",
journal = "IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020: Book of Abstracts; 2020 Nov 12-14; Banja Luka, Bosnia and Herzegovinia",
title = "Correlation patterns in three Lamium species grown in two light and three density treatments",
pages = "126",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5450"
}

Barišić Klisarić, N., Avramov, S., Miljković, D., Živković, U.,& Tarasjev, A.. (2020). Correlation patterns in three Lamium species grown in two light and three density treatments. in IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020: Book of Abstracts; 2020 Nov 12-14; Banja Luka, Bosnia and Herzegovinia
Banja Luka: Faculty of Natural Sciences and Mathematics, University of Banja Luka., 126.
https://hdl.handle.net/21.15107/rcub_ibiss_5450

Barišić Klisarić N, Avramov S, Miljković D, Živković U, Tarasjev A. Correlation patterns in three Lamium species grown in two light and three density treatments. in IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020: Book of Abstracts; 2020 Nov 12-14; Banja Luka, Bosnia and Herzegovinia. 2020;:126.
https://hdl.handle.net/21.15107/rcub_ibiss_5450 .

Barišić Klisarić, Nataša, Avramov, Stevan, Miljković, Danijela, Živković, Uroš, Tarasjev, Aleksej, "Correlation patterns in three Lamium species grown in two light and three density treatments" in IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020: Book of Abstracts; 2020 Nov 12-14; Banja Luka, Bosnia and Herzegovinia (2020):126,
https://hdl.handle.net/21.15107/rcub_ibiss_5450 .

TY  - CONF
AU  - Živković, Uroš
AU  - Avramov, Stevan
AU  - Barišić Klisarić, Nataša
AU  - Miljković, Danijela
AU  - Tubić, Ljiljana
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Tarasjev, Aleksej
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4115
AB  - Higher plants have developed protection mechanisms in order to diminish the
effects caused by the presence of free radicals generated during high
irradiance, and one of the detoxification mechanisms is the synthesis of
secondary metabolites. In this study we investigated the amount and pattern
of individual phenotypic responses (represented through targeted phenolics
content in 68 genotypes of Iris variegata) to seasonal changes in
environmental conditions under two experimental light regimes. The goal was
to examine how environmental stressors mold the interrelationships between
metabolite traits. Genotypes of I. variegata were collected in Deliblato sands
Special Nature Reserve in Serbia, from two types of natural habitats (exposed
and shade) and transferred into the experimental field conditions providing
either 1. high light intensity and higher red / far red light ratio and 2. low light
intensity and lower red / far red light ratio. After period of acclimatization
leaves were collected during spring, summer and fall of one experimental year
and subjected to UHPLC/qqqMS quantification of 10 selected phenolic
compounds belonging to the groups of phenolic acids, flavonoids and
xanthones. The correlations between all studied compounds within two light
treatments and three seasons were in almost all cases significant and positive.
Number of statistically significant correlations markedly decreased through
the vegetative period in both light treatments. The experiment was conducted
on a large number of genotypes of I. variegata and points to the need of
involving a number of factors in future ecological and evolutionary researches.
PB  - Banja Luka: Faculty of Natural Sciences and Mathematics, University of Banja Luka
C3  - IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020
T1  - Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions
SP  - 127
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4115
ER  - 

@conference{
author = "Živković, Uroš and Avramov, Stevan and Barišić Klisarić, Nataša and Miljković, Danijela and Tubić, Ljiljana and Mišić, Danijela and Šiler, Branislav and Tarasjev, Aleksej",
year = "2020",
abstract = "Higher plants have developed protection mechanisms in order to diminish the
effects caused by the presence of free radicals generated during high
irradiance, and one of the detoxification mechanisms is the synthesis of
secondary metabolites. In this study we investigated the amount and pattern
of individual phenotypic responses (represented through targeted phenolics
content in 68 genotypes of Iris variegata) to seasonal changes in
environmental conditions under two experimental light regimes. The goal was
to examine how environmental stressors mold the interrelationships between
metabolite traits. Genotypes of I. variegata were collected in Deliblato sands
Special Nature Reserve in Serbia, from two types of natural habitats (exposed
and shade) and transferred into the experimental field conditions providing
either 1. high light intensity and higher red / far red light ratio and 2. low light
intensity and lower red / far red light ratio. After period of acclimatization
leaves were collected during spring, summer and fall of one experimental year
and subjected to UHPLC/qqqMS quantification of 10 selected phenolic
compounds belonging to the groups of phenolic acids, flavonoids and
xanthones. The correlations between all studied compounds within two light
treatments and three seasons were in almost all cases significant and positive.
Number of statistically significant correlations markedly decreased through
the vegetative period in both light treatments. The experiment was conducted
on a large number of genotypes of I. variegata and points to the need of
involving a number of factors in future ecological and evolutionary researches.",
publisher = "Banja Luka: Faculty of Natural Sciences and Mathematics, University of Banja Luka",
journal = "IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020",
title = "Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions",
pages = "127",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4115"
}

Živković, U., Avramov, S., Barišić Klisarić, N., Miljković, D., Tubić, L., Mišić, D., Šiler, B.,& Tarasjev, A.. (2020). Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions. in IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020
Banja Luka: Faculty of Natural Sciences and Mathematics, University of Banja Luka., 127.
https://hdl.handle.net/21.15107/rcub_ibiss_4115

Živković U, Avramov S, Barišić Klisarić N, Miljković D, Tubić L, Mišić D, Šiler B, Tarasjev A. Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions. in IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020. 2020;:127.
https://hdl.handle.net/21.15107/rcub_ibiss_4115 .

Živković, Uroš, Avramov, Stevan, Barišić Klisarić, Nataša, Miljković, Danijela, Tubić, Ljiljana, Mišić, Danijela, Šiler, Branislav, Tarasjev, Aleksej, "Seasonal variability in correlation patterns among Iris variegata L. genotypes growing in contrasting light conditions" in IV symposium of biologists and ecologists of Republic of Srpska with international participation – SBERS2020 (2020):127,
https://hdl.handle.net/21.15107/rcub_ibiss_4115 .

TY  - JOUR
AU  - Barišić Klisarić, Nataša
AU  - Miljković, Danijela
AU  - Avramov, Stevan
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2019
UR  - https://www.mdpi.com/2073-8994/11/6/818
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3371
AB  - In this study we compared the biomonitoring potential of various types of flower asymmetry indices in Iris pumila (Dwarf Bearded Iris). We chose 197 naturally growing clones from the arid steppe habitat in the largest sandy area in Europe (Deliblato Sands Nature Reserve), and we transplanted two replicates of each clone to a polluted highway site with a heavy traffic flow. After a period of acclimatization, lower levels of photosynthetic pigment concentrations and higher stomatal density and specific leaf area in transplants verified that the chosen highway site was indeed more stressful and therefore suitable for estimation of the flower asymmetry biomonitoring potential. We analyzed radially and bilaterally symmetrical flower structures (radial fluctuating asymmetry (RA) and bilateral fluctuating asymmetry (FA)) on three perianth parts—falls, standards, and styles—and calculated various asymmetry indices based on linear and geometric morphometrics. Despite utilizing a heavily polluted environment and fairly large sample sizes, only one asymmetry index was significantly higher on the polluted site with demonstrated stressful effects on utilized plants, indicating that flower asymmetry was not an efficient method for biomonitoring in the case of I. pumila RA and FA indices.
T2  - Symmetry
T2  - Symmetry
T1  - Radial and Bilateral Fluctuating Asymmetry of Iris pumila Flowers as Indicators of Environmental Stress
IS  - 6
VL  - 11
DO  - 10.3390/sym11060818
SP  - 818
ER  - 

@article{
author = "Barišić Klisarić, Nataša and Miljković, Danijela and Avramov, Stevan and Živković, Uroš and Tarasjev, Aleksej",
year = "2019",
abstract = "In this study we compared the biomonitoring potential of various types of flower asymmetry indices in Iris pumila (Dwarf Bearded Iris). We chose 197 naturally growing clones from the arid steppe habitat in the largest sandy area in Europe (Deliblato Sands Nature Reserve), and we transplanted two replicates of each clone to a polluted highway site with a heavy traffic flow. After a period of acclimatization, lower levels of photosynthetic pigment concentrations and higher stomatal density and specific leaf area in transplants verified that the chosen highway site was indeed more stressful and therefore suitable for estimation of the flower asymmetry biomonitoring potential. We analyzed radially and bilaterally symmetrical flower structures (radial fluctuating asymmetry (RA) and bilateral fluctuating asymmetry (FA)) on three perianth parts—falls, standards, and styles—and calculated various asymmetry indices based on linear and geometric morphometrics. Despite utilizing a heavily polluted environment and fairly large sample sizes, only one asymmetry index was significantly higher on the polluted site with demonstrated stressful effects on utilized plants, indicating that flower asymmetry was not an efficient method for biomonitoring in the case of I. pumila RA and FA indices.",
journal = "Symmetry, Symmetry",
title = "Radial and Bilateral Fluctuating Asymmetry of Iris pumila Flowers as Indicators of Environmental Stress",
number = "6",
volume = "11",
doi = "10.3390/sym11060818",
pages = "818"
}

Barišić Klisarić, N., Miljković, D., Avramov, S., Živković, U.,& Tarasjev, A.. (2019). Radial and Bilateral Fluctuating Asymmetry of Iris pumila Flowers as Indicators of Environmental Stress. in Symmetry, 11(6), 818.
https://doi.org/10.3390/sym11060818

Barišić Klisarić N, Miljković D, Avramov S, Živković U, Tarasjev A. Radial and Bilateral Fluctuating Asymmetry of Iris pumila Flowers as Indicators of Environmental Stress. in Symmetry. 2019;11(6):818.
doi:10.3390/sym11060818 .

Barišić Klisarić, Nataša, Miljković, Danijela, Avramov, Stevan, Živković, Uroš, Tarasjev, Aleksej, "Radial and Bilateral Fluctuating Asymmetry of Iris pumila Flowers as Indicators of Environmental Stress" in Symmetry, 11, no. 6 (2019):818,
https://doi.org/10.3390/sym11060818 . .

TY  - CONF
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
AU  - Marius, Olđa
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6670
AB  - The effectiveness of roadside corridors for the migration of invasive plant species has often been linked to altered site conditions along roads due to increased disturbance, thus reducing the competitive strength of the native roadside flora. The invasion histories of some roadside plant species indicate that, in addition to site-related changes, traffic also promotes invasiveness. Asclepias syriaca (common milkweed) is a fast-growing competitor with effective clonal spreading and seed dispersal ability. A single common milkweed plant can produce a few thousand seeds with long, white flossy hairs that can easily be carried by wind. A. syriaca is common along the borders of the protected area - Deliblato sands, and is spreading into this area, mostly along the roads. Impact of traffic and accompanying human activities as dispersal vectors on seed deposition of A. syriaca in Deliblato sands has been investigated and our results demonstrate that, besides altered site conditions and abundance along particular Special Nature Reserve border, traffic-borne dispersal is an important cause of the higher incidence of A. syriaca on roadside verges. There was significantly higher number of A. syriaca plants along roads with heavier traffic. Also, higher frequency of road use results in higher incidence of new establishment of A. syriaca. Transport and human activities around roads can also explain the occurrence of isolated founder populations and discontinuous distributional patterns of common milkweed in this area.
PB  - Beograd: Serbian Plant Physiology Society
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Impact of traffic-borne dispersal of invasive Asclepias syriaca L.in Special Nature Reserve Deliblato sands
SP  - 95
EP  - 96
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6670
ER  - 

@conference{
author = "Avramov, Stevan and Miljković, Danijela and Barišić Klisarić, Nataša and Živković, Uroš and Tarasjev, Aleksej and Marius, Olđa",
year = "2018",
abstract = "The effectiveness of roadside corridors for the migration of invasive plant species has often been linked to altered site conditions along roads due to increased disturbance, thus reducing the competitive strength of the native roadside flora. The invasion histories of some roadside plant species indicate that, in addition to site-related changes, traffic also promotes invasiveness. Asclepias syriaca (common milkweed) is a fast-growing competitor with effective clonal spreading and seed dispersal ability. A single common milkweed plant can produce a few thousand seeds with long, white flossy hairs that can easily be carried by wind. A. syriaca is common along the borders of the protected area - Deliblato sands, and is spreading into this area, mostly along the roads. Impact of traffic and accompanying human activities as dispersal vectors on seed deposition of A. syriaca in Deliblato sands has been investigated and our results demonstrate that, besides altered site conditions and abundance along particular Special Nature Reserve border, traffic-borne dispersal is an important cause of the higher incidence of A. syriaca on roadside verges. There was significantly higher number of A. syriaca plants along roads with heavier traffic. Also, higher frequency of road use results in higher incidence of new establishment of A. syriaca. Transport and human activities around roads can also explain the occurrence of isolated founder populations and discontinuous distributional patterns of common milkweed in this area.",
publisher = "Beograd: Serbian Plant Physiology Society",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Impact of traffic-borne dispersal of invasive Asclepias syriaca L.in Special Nature Reserve Deliblato sands",
pages = "95-96",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6670"
}

Avramov, S., Miljković, D., Barišić Klisarić, N., Živković, U., Tarasjev, A.,& Marius, O.. (2018). Impact of traffic-borne dispersal of invasive Asclepias syriaca L.in Special Nature Reserve Deliblato sands. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Beograd: Serbian Plant Physiology Society., 95-96.
https://hdl.handle.net/21.15107/rcub_ibiss_6670

Avramov S, Miljković D, Barišić Klisarić N, Živković U, Tarasjev A, Marius O. Impact of traffic-borne dispersal of invasive Asclepias syriaca L.in Special Nature Reserve Deliblato sands. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:95-96.
https://hdl.handle.net/21.15107/rcub_ibiss_6670 .

Avramov, Stevan, Miljković, Danijela, Barišić Klisarić, Nataša, Živković, Uroš, Tarasjev, Aleksej, Marius, Olđa, "Impact of traffic-borne dispersal of invasive Asclepias syriaca L.in Special Nature Reserve Deliblato sands" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):95-96,
https://hdl.handle.net/21.15107/rcub_ibiss_6670 .

TY  - CONF
AU  - Živković, Uroš
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Avramov, Stevan
AU  - Tarasjev, Aleksej
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6675
AB  - Obrasci vaskularne arhitekture listova i latica u izvesnoj meri zavise od sredine u kojoj se odvija razviće biljaka. Provodni sistem hranjivih materija, vode i produkata fotosinteze (floem i ksilem) je organizovan u vaskularne snopove ili vene, koji ujedno ima i funkciju mehaničke potpore biljnih organa, listova i delova cveta.1 Makroskopska struktura anatomije vena i areola (površine mezofilnog tkiva koje se nalaze između vena višeg reda) vegetativnih i reproduktivnh organa biljaka je važan deo istraživanja ekologije biljaka. Jedna od metoda je i analiza slika primenom LEAF GUI softverskog grafičkog programa.2 Tehnička obrada slika objekata na kojima se radi analiza arhitekture nerava omogućava dobijanje statističkih podataka dimenzija vena i areola. Dobijene matematičke veze između veličine objekta i obrazaca nervature imaju potencijal da objasne uticaj sredinskih i genetičkih faktora njihovog formiranja. Analizom slika dobijaju se informacije o arhitekturi vena, njihovom broju, gustini, granjanju, volumenu, površini areola koje vene okružuju, kao i broju krajeva grana malih vena unutar njih. Dosadašnji rezultati istraživanja vaskularne arhitekture listova još uvek ne objašnjavaju u potpunosti uticaj faktora koji određuju njeno formiranje, a još manje je podataka o arhitekturi vena delova cveta. Cilj sprovedenog istraživanja je primena LEAF GUI softvera u proceni uticaja sredinskih faktora (intenzitet svetlosti) i procena genetičke varijabilnosti vaskularne arhitekture na folovima cveta I. variegata. Insekti tokom oprašivanja sleću na fol, deo cveta specifične pigmentacije vaskularne arhitekture koja ima vizuelnu ulogu u privlačenju oprašivača.3 Prema dosadašnjim saznanjima ovo je prva primena LEAF GUI softvera za analizu vaskularne arhitekture latica cveta.
PB  - Belgrade: Serbian Biological Society
C3  - Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
T1  - Metoda obrade slike za ispitivanje vaskularne arhitekture lista: prva studija analize cveta Iris variegata L. (Iridaceae)
SP  - 141
EP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6675
ER  - 

@conference{
author = "Živković, Uroš and Miljković, Danijela and Barišić Klisarić, Nataša and Avramov, Stevan and Tarasjev, Aleksej",
year = "2018",
abstract = "Obrasci vaskularne arhitekture listova i latica u izvesnoj meri zavise od sredine u kojoj se odvija razviće biljaka. Provodni sistem hranjivih materija, vode i produkata fotosinteze (floem i ksilem) je organizovan u vaskularne snopove ili vene, koji ujedno ima i funkciju mehaničke potpore biljnih organa, listova i delova cveta.1 Makroskopska struktura anatomije vena i areola (površine mezofilnog tkiva koje se nalaze između vena višeg reda) vegetativnih i reproduktivnh organa biljaka je važan deo istraživanja ekologije biljaka. Jedna od metoda je i analiza slika primenom LEAF GUI softverskog grafičkog programa.2 Tehnička obrada slika objekata na kojima se radi analiza arhitekture nerava omogućava dobijanje statističkih podataka dimenzija vena i areola. Dobijene matematičke veze između veličine objekta i obrazaca nervature imaju potencijal da objasne uticaj sredinskih i genetičkih faktora njihovog formiranja. Analizom slika dobijaju se informacije o arhitekturi vena, njihovom broju, gustini, granjanju, volumenu, površini areola koje vene okružuju, kao i broju krajeva grana malih vena unutar njih. Dosadašnji rezultati istraživanja vaskularne arhitekture listova još uvek ne objašnjavaju u potpunosti uticaj faktora koji određuju njeno formiranje, a još manje je podataka o arhitekturi vena delova cveta. Cilj sprovedenog istraživanja je primena LEAF GUI softvera u proceni uticaja sredinskih faktora (intenzitet svetlosti) i procena genetičke varijabilnosti vaskularne arhitekture na folovima cveta I. variegata. Insekti tokom oprašivanja sleću na fol, deo cveta specifične pigmentacije vaskularne arhitekture koja ima vizuelnu ulogu u privlačenju oprašivača.3 Prema dosadašnjim saznanjima ovo je prva primena LEAF GUI softvera za analizu vaskularne arhitekture latica cveta.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija",
title = "Metoda obrade slike za ispitivanje vaskularne arhitekture lista: prva studija analize cveta Iris variegata L. (Iridaceae)",
pages = "141-141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6675"
}

Živković, U., Miljković, D., Barišić Klisarić, N., Avramov, S.,& Tarasjev, A.. (2018). Metoda obrade slike za ispitivanje vaskularne arhitekture lista: prva studija analize cveta Iris variegata L. (Iridaceae). in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija
Belgrade: Serbian Biological Society., 141-141.
https://hdl.handle.net/21.15107/rcub_ibiss_6675

Živković U, Miljković D, Barišić Klisarić N, Avramov S, Tarasjev A. Metoda obrade slike za ispitivanje vaskularne arhitekture lista: prva studija analize cveta Iris variegata L. (Iridaceae). in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija. 2018;:141-141.
https://hdl.handle.net/21.15107/rcub_ibiss_6675 .

Živković, Uroš, Miljković, Danijela, Barišić Klisarić, Nataša, Avramov, Stevan, Tarasjev, Aleksej, "Metoda obrade slike za ispitivanje vaskularne arhitekture lista: prva studija analize cveta Iris variegata L. (Iridaceae)" in Drugi kongres biologa Srbije; 2018 Sep 25-30; Kladovo, Srbija (2018):141-141,
https://hdl.handle.net/21.15107/rcub_ibiss_6675 .

TY  - CONF
AU  - Tarasjev, Aleksej
AU  - Barišić Klisarić, Nataša
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Živković, Uroš
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6669
AB  - Dwarf bearded iris - Iris pumila L. is a perennial clonal plant that exhibits huge flower colour
polymorphism with white, yellow and various shades of purple and violet flowers. Maintenance
of such polymorphism is very interesting evolutionary question in its own right. Moreover, flower
colour is also used in determining individual clones, their size and population diversity - all very
important aspects of further population and evolutionary studies on this model system. However,
visual identification or use of digitalized images, while being a necessary first step, is not sufficient
for this task. They are often subjective, dependent on light conditions, and covering only part of
the spectrum - one that is visible to humans. In published papers that utilized flower colour in I.
pumila, number of analyzed different colour variants ranged from nine to only three colour classes.
Chemical analysis of different colour variants is a very useful tool and has also been applied, but it
is hardly applicable for most population level analyses. We therefore consider portable reflectance
spectrometer as the best available tool for those multiple tasks, and present preliminary results
of its application in situ with wavelengths chosen to match the visible spectrum of I. pumila main
pollinators.
PB  - Beograd: Serbian Plant Physiology Society
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Flower color polymorphism in Iris pumila - Why we definitely need in situ reflectance spectroscopy in population analysis and evolutionary studies of this model system
SP  - 85
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6669
ER  - 

@conference{
author = "Tarasjev, Aleksej and Barišić Klisarić, Nataša and Avramov, Stevan and Miljković, Danijela and Živković, Uroš",
year = "2018",
abstract = "Dwarf bearded iris - Iris pumila L. is a perennial clonal plant that exhibits huge flower colour
polymorphism with white, yellow and various shades of purple and violet flowers. Maintenance
of such polymorphism is very interesting evolutionary question in its own right. Moreover, flower
colour is also used in determining individual clones, their size and population diversity - all very
important aspects of further population and evolutionary studies on this model system. However,
visual identification or use of digitalized images, while being a necessary first step, is not sufficient
for this task. They are often subjective, dependent on light conditions, and covering only part of
the spectrum - one that is visible to humans. In published papers that utilized flower colour in I.
pumila, number of analyzed different colour variants ranged from nine to only three colour classes.
Chemical analysis of different colour variants is a very useful tool and has also been applied, but it
is hardly applicable for most population level analyses. We therefore consider portable reflectance
spectrometer as the best available tool for those multiple tasks, and present preliminary results
of its application in situ with wavelengths chosen to match the visible spectrum of I. pumila main
pollinators.",
publisher = "Beograd: Serbian Plant Physiology Society",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Flower color polymorphism in Iris pumila - Why we definitely need in situ reflectance spectroscopy in population analysis and evolutionary studies of this model system",
pages = "85-85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6669"
}

Tarasjev, A., Barišić Klisarić, N., Avramov, S., Miljković, D.,& Živković, U.. (2018). Flower color polymorphism in Iris pumila - Why we definitely need in situ reflectance spectroscopy in population analysis and evolutionary studies of this model system. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Beograd: Serbian Plant Physiology Society., 85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6669

Tarasjev A, Barišić Klisarić N, Avramov S, Miljković D, Živković U. Flower color polymorphism in Iris pumila - Why we definitely need in situ reflectance spectroscopy in population analysis and evolutionary studies of this model system. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:85-85.
https://hdl.handle.net/21.15107/rcub_ibiss_6669 .

Tarasjev, Aleksej, Barišić Klisarić, Nataša, Avramov, Stevan, Miljković, Danijela, Živković, Uroš, "Flower color polymorphism in Iris pumila - Why we definitely need in situ reflectance spectroscopy in population analysis and evolutionary studies of this model system" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):85-85,
https://hdl.handle.net/21.15107/rcub_ibiss_6669 .

TY  - CONF
AU  - Živković, Uroš
AU  - Avramov, Stevan
AU  - Barišić Klisarić, Nataša
AU  - Miljković, Danijela
AU  - Tubić, Ljiljana
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Tarasjev, Aleksej
PY  - 2018
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6494
AB  - During their evolution plants developed a set of mechanisms to adapt to the varying environmental conditions. Light is one of the most dynamic components of the terrestrial environment
that affects plant physiology and development. Optimization of light harvesting for photoautotrophic growth inevitably induces specific adjustments in all aspects of plant phenotype: morphology, anatomy, phytochemical composition, flowering phenology etc. As sessile organisms
capable of perceiving quantitative and qualitative features of light surroundings, plants need to
be particularly plastic in their response to different light environments. For this study genotypes of Iris variegata that occupy different light habitats in Deliblato sands were selected: a) open habitats
where they were exposed to full sunlight and b) woodland understories with lower light intensity
and changed light quality (vegetative shade). Specimens of those I. variegata genotypes were
grown under two experimental light treatments: 1.) high intensity and higher red-far red light ratio
and 2.) low intensity and lower red-far red light ratio. Leaves were collected during spring, summer
and autumn of one experimental year, dried in silica gel and extracted with methanol. Samples
were subjected to UHPLC/qqqMS profiling of phenolics (phenolic acids, flavonoids, and xanthones), and subsequently to chemometric analysis. Results showed that dissimilar light conditions
in applied treatments markedly affected I. variegata phenolics composition. Repeated measures
model ANOVA revealed a significant effect of seasons for all analyzed phenolics. Concentrations of
half of the analyzed chemical compounds were significantly different under two light treatments.
The correlations between traits were in almost all cases significant and positive.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
T1  - Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions
SP  - 97
EP  - 98
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6494
ER  - 

@conference{
author = "Živković, Uroš and Avramov, Stevan and Barišić Klisarić, Nataša and Miljković, Danijela and Tubić, Ljiljana and Mišić, Danijela and Šiler, Branislav and Tarasjev, Aleksej",
year = "2018",
abstract = "During their evolution plants developed a set of mechanisms to adapt to the varying environmental conditions. Light is one of the most dynamic components of the terrestrial environment
that affects plant physiology and development. Optimization of light harvesting for photoautotrophic growth inevitably induces specific adjustments in all aspects of plant phenotype: morphology, anatomy, phytochemical composition, flowering phenology etc. As sessile organisms
capable of perceiving quantitative and qualitative features of light surroundings, plants need to
be particularly plastic in their response to different light environments. For this study genotypes of Iris variegata that occupy different light habitats in Deliblato sands were selected: a) open habitats
where they were exposed to full sunlight and b) woodland understories with lower light intensity
and changed light quality (vegetative shade). Specimens of those I. variegata genotypes were
grown under two experimental light treatments: 1.) high intensity and higher red-far red light ratio
and 2.) low intensity and lower red-far red light ratio. Leaves were collected during spring, summer
and autumn of one experimental year, dried in silica gel and extracted with methanol. Samples
were subjected to UHPLC/qqqMS profiling of phenolics (phenolic acids, flavonoids, and xanthones), and subsequently to chemometric analysis. Results showed that dissimilar light conditions
in applied treatments markedly affected I. variegata phenolics composition. Repeated measures
model ANOVA revealed a significant effect of seasons for all analyzed phenolics. Concentrations of
half of the analyzed chemical compounds were significantly different under two light treatments.
The correlations between traits were in almost all cases significant and positive.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia",
title = "Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions",
pages = "97-98",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6494"
}

Živković, U., Avramov, S., Barišić Klisarić, N., Miljković, D., Tubić, L., Mišić, D., Šiler, B.,& Tarasjev, A.. (2018). Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 97-98.
https://hdl.handle.net/21.15107/rcub_ibiss_6494

Živković U, Avramov S, Barišić Klisarić N, Miljković D, Tubić L, Mišić D, Šiler B, Tarasjev A. Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions. in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia. 2018;:97-98.
https://hdl.handle.net/21.15107/rcub_ibiss_6494 .

Živković, Uroš, Avramov, Stevan, Barišić Klisarić, Nataša, Miljković, Danijela, Tubić, Ljiljana, Mišić, Danijela, Šiler, Branislav, Tarasjev, Aleksej, "Seasonal variability in leaf chemistry of Iris variegata L. genotypes growing in contrasting light conditions" in Book of abstracts. 3rd International Conference on Plant Biology (22nd SPPS Meeting); 2018 Jun 9-12; Belgrade, Serbia (2018):97-98,
https://hdl.handle.net/21.15107/rcub_ibiss_6494 .

TY  - JOUR
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2017
UR  - http://doi.wiley.com/10.1111/1442-1984.12171
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2738
AB  - To better understand what directs and limits the evolution of phenotype, constraints in the realization of the optimal phenotype need to be addressed. That includes estimations of variability of adaptively important traits as well as their correlation structures, but also evaluation of how they are affected by relevant environmental conditions and development phases. The aims of this study were to analyze phenotypic plasticity, genetic variability and correlation structures of important Iris pumila leaf traits in different light environments and ontogenetic phases, and estimate its evolutionary potential. Stomatal density, specific leaf area, total chlorophyll concentration and chlorophyll a/b ratio were analyzed on I. pumila full-sib families in the seedling phase and on the same plants after 3years of growth in contrasting light conditions typical for ontogenetic stage in question. There was a significant phenotypic plasticity in both ontogenetic stages, but significant genetic variability was detected only for chlorophyll concentrations. Correlations of the same trait between different stages were weak due to changes in environmental conditions and difference in ontogenetic reaction norms of different genotypes. Ontogenetic variability of correlation structures was detected, where correlations and integration were higher in seedlings compared with adult plants 3years later. Correlations were affected by environmental conditions, with integration being higher in the lower light conditions, but correlations between phases being stronger in the higher light treatment. These findings demonstrated that the analyzed traits can be selected and can mostly evolve independently in different environments and ontogenetic stages, with low genetic variability as a potentially main constraint.
T2  - Plant Species Biology
T1  - Ontogenetic plasticity of anatomical and ecophysiological traits and their correlations in Iris pumila plants grown in contrasting light conditions
IS  - 4
VL  - 32
DO  - 10.1111/1442-1984.12171
SP  - 392
EP  - 402
ER  - 

@article{
author = "Avramov, Stevan and Miljković, Danijela and Barišić Klisarić, Nataša and Živković, Uroš and Tarasjev, Aleksej",
year = "2017",
abstract = "To better understand what directs and limits the evolution of phenotype, constraints in the realization of the optimal phenotype need to be addressed. That includes estimations of variability of adaptively important traits as well as their correlation structures, but also evaluation of how they are affected by relevant environmental conditions and development phases. The aims of this study were to analyze phenotypic plasticity, genetic variability and correlation structures of important Iris pumila leaf traits in different light environments and ontogenetic phases, and estimate its evolutionary potential. Stomatal density, specific leaf area, total chlorophyll concentration and chlorophyll a/b ratio were analyzed on I. pumila full-sib families in the seedling phase and on the same plants after 3years of growth in contrasting light conditions typical for ontogenetic stage in question. There was a significant phenotypic plasticity in both ontogenetic stages, but significant genetic variability was detected only for chlorophyll concentrations. Correlations of the same trait between different stages were weak due to changes in environmental conditions and difference in ontogenetic reaction norms of different genotypes. Ontogenetic variability of correlation structures was detected, where correlations and integration were higher in seedlings compared with adult plants 3years later. Correlations were affected by environmental conditions, with integration being higher in the lower light conditions, but correlations between phases being stronger in the higher light treatment. These findings demonstrated that the analyzed traits can be selected and can mostly evolve independently in different environments and ontogenetic stages, with low genetic variability as a potentially main constraint.",
journal = "Plant Species Biology",
title = "Ontogenetic plasticity of anatomical and ecophysiological traits and their correlations in Iris pumila plants grown in contrasting light conditions",
number = "4",
volume = "32",
doi = "10.1111/1442-1984.12171",
pages = "392-402"
}

Avramov, S., Miljković, D., Barišić Klisarić, N., Živković, U.,& Tarasjev, A.. (2017). Ontogenetic plasticity of anatomical and ecophysiological traits and their correlations in Iris pumila plants grown in contrasting light conditions. in Plant Species Biology, 32(4), 392-402.
https://doi.org/10.1111/1442-1984.12171

Avramov S, Miljković D, Barišić Klisarić N, Živković U, Tarasjev A. Ontogenetic plasticity of anatomical and ecophysiological traits and their correlations in Iris pumila plants grown in contrasting light conditions. in Plant Species Biology. 2017;32(4):392-402.
doi:10.1111/1442-1984.12171 .

Avramov, Stevan, Miljković, Danijela, Barišić Klisarić, Nataša, Živković, Uroš, Tarasjev, Aleksej, "Ontogenetic plasticity of anatomical and ecophysiological traits and their correlations in Iris pumila plants grown in contrasting light conditions" in Plant Species Biology, 32, no. 4 (2017):392-402,
https://doi.org/10.1111/1442-1984.12171 . .

TY  - JOUR
AU  - Lazić, Marko M.
AU  - Carretero, Miguel A.
AU  - Živković, Uroš
AU  - Crnobrnja-Isailović, Jelka
PY  - 2017
UR  - http://www.salamandra-journal.com/index.php/home/contents/2017-vol-53/1800-lazic-m-m-m-a-carretero-u-zivkovic-j-crnobrnja-isailovic
UR  - http://www.salamandra-journal.com/index.php/home/contents/2017-vol-53/1800-lazic-m-m-m-a-carretero-u-zivkovic-j-crnobrnja-i
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2581
UR  - http://www.salamandra-journal.com/index.php/home/contents/2017-vol-53/1800-lazic-m-m-m-a-carretero-u-zivkovic-j-crnobrnja-isailovic
AB  - Animals living in urban areas experience additional stress compared to those inhabiting more natural habitats. This could influence their physical state and ability to cope with parasites. Here, effects of environmental disturbances on body condition and prevalence and load of blood parasites in the common wall lizard (Podarcis muralis) were investigated by comparing these indices between five urban and five rural populations. The physical condition index was lower in urban areas, and females were the most affected sex. This suggests significant fitness costs, as survival and reproductive output are often tightly linked to body condition. Prevalence of blood parasites was more variable in urban (2.5–100%) compared to rural (50.0–75.6%) populations, with no statistically significant differences between the two habitats. Prevalence of blood parasites increased with body size. Parasite load was significantly higher in urban lizards, suggesting strong effects of urbanisation on host–parasite interaction. An increased concentration of blood parasites should affect fitness since various aspects of physiology are compromised in parasitised animals. Larger animals were more frequently and more severely infected, most likely due to their being longer and more frequently exposed to parasites. No differences between sexes in both prevalence and intensity were found, suggesting equal susceptibility or exposure to parasites.
T2  - Salamandra
T1  - City life has fitness costs: Reduced body condition and increased parasite load in urban common wall lizards, Podarcis muralis
IS  - 1
VL  - 53
SP  - 10
EP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2581
ER  - 

@article{
author = "Lazić, Marko M. and Carretero, Miguel A. and Živković, Uroš and Crnobrnja-Isailović, Jelka",
year = "2017",
abstract = "Animals living in urban areas experience additional stress compared to those inhabiting more natural habitats. This could influence their physical state and ability to cope with parasites. Here, effects of environmental disturbances on body condition and prevalence and load of blood parasites in the common wall lizard (Podarcis muralis) were investigated by comparing these indices between five urban and five rural populations. The physical condition index was lower in urban areas, and females were the most affected sex. This suggests significant fitness costs, as survival and reproductive output are often tightly linked to body condition. Prevalence of blood parasites was more variable in urban (2.5–100%) compared to rural (50.0–75.6%) populations, with no statistically significant differences between the two habitats. Prevalence of blood parasites increased with body size. Parasite load was significantly higher in urban lizards, suggesting strong effects of urbanisation on host–parasite interaction. An increased concentration of blood parasites should affect fitness since various aspects of physiology are compromised in parasitised animals. Larger animals were more frequently and more severely infected, most likely due to their being longer and more frequently exposed to parasites. No differences between sexes in both prevalence and intensity were found, suggesting equal susceptibility or exposure to parasites.",
journal = "Salamandra",
title = "City life has fitness costs: Reduced body condition and increased parasite load in urban common wall lizards, Podarcis muralis",
number = "1",
volume = "53",
pages = "10-17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2581"
}

Lazić, M. M., Carretero, M. A., Živković, U.,& Crnobrnja-Isailović, J.. (2017). City life has fitness costs: Reduced body condition and increased parasite load in urban common wall lizards, Podarcis muralis. in Salamandra, 53(1), 10-17.
https://hdl.handle.net/21.15107/rcub_ibiss_2581

Lazić MM, Carretero MA, Živković U, Crnobrnja-Isailović J. City life has fitness costs: Reduced body condition and increased parasite load in urban common wall lizards, Podarcis muralis. in Salamandra. 2017;53(1):10-17.
https://hdl.handle.net/21.15107/rcub_ibiss_2581 .

Lazić, Marko M., Carretero, Miguel A., Živković, Uroš, Crnobrnja-Isailović, Jelka, "City life has fitness costs: Reduced body condition and increased parasite load in urban common wall lizards, Podarcis muralis" in Salamandra, 53, no. 1 (2017):10-17,
https://hdl.handle.net/21.15107/rcub_ibiss_2581 .

TY  - JOUR
AU  - Barišić-Klisarić, Nataša
AU  - Miljković, Danijela
AU  - Avramov, Stevan
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2016
UR  - http://www.doiserbia.nb.rs/img/doi/0354-4664/2016 OnLine-First/0354-46641600072B.pdf
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/1257
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2492
AB  - In light of the increasing need for appropriate, cost-effective detection methods of anthropogenic pollution, we evaluated the biomonitoring potential of flower developmental instability (DI) on a widely planted decorative species, Iris germanica, under in situ conditions. DI was measured by fluctuating and radial asymmetries of parts of Iris germanica perianth (810 fall lengths and widths), from clones already growing in two distinct types of habitats with contrasting levels of anthropogenic pollution: in unpolluted (rural) areas, Novi Banovci, Stari Banovci and Belegiš (flowers from 137 clones sampled), and in a polluted (urban) Belgrade metropolitan area (flowers from 133 clones sampled). Our results revealed significantly higher flower radial asymmetry in the polluted habitats compared to unpolluted ones (for three out of four univariate indices, as well as both multivariate ones), but failed to detect a similar effect on fluctuating asymmetry indices. The results of our study therefore demonstrate the potential of DI (when estimated by flower radial asymmetry) in Iris germanica as a cost-effective biomonitoring method for in situ pollution detection based on readily measurable flower parts and moderate sample sizes.
T2  - Archives of Biological Sciences
T1  - Developmental instability in German Iris flower as a potential biomonitoring method
IS  - 4
VL  - 68
DO  - 10.2298/ABS151229072B
SP  - 837
EP  - 844
ER  - 

@article{
author = "Barišić-Klisarić, Nataša and Miljković, Danijela and Avramov, Stevan and Živković, Uroš and Tarasjev, Aleksej",
year = "2016",
abstract = "In light of the increasing need for appropriate, cost-effective detection methods of anthropogenic pollution, we evaluated the biomonitoring potential of flower developmental instability (DI) on a widely planted decorative species, Iris germanica, under in situ conditions. DI was measured by fluctuating and radial asymmetries of parts of Iris germanica perianth (810 fall lengths and widths), from clones already growing in two distinct types of habitats with contrasting levels of anthropogenic pollution: in unpolluted (rural) areas, Novi Banovci, Stari Banovci and Belegiš (flowers from 137 clones sampled), and in a polluted (urban) Belgrade metropolitan area (flowers from 133 clones sampled). Our results revealed significantly higher flower radial asymmetry in the polluted habitats compared to unpolluted ones (for three out of four univariate indices, as well as both multivariate ones), but failed to detect a similar effect on fluctuating asymmetry indices. The results of our study therefore demonstrate the potential of DI (when estimated by flower radial asymmetry) in Iris germanica as a cost-effective biomonitoring method for in situ pollution detection based on readily measurable flower parts and moderate sample sizes.",
journal = "Archives of Biological Sciences",
title = "Developmental instability in German Iris flower as a potential biomonitoring method",
number = "4",
volume = "68",
doi = "10.2298/ABS151229072B",
pages = "837-844"
}

Barišić-Klisarić, N., Miljković, D., Avramov, S., Živković, U.,& Tarasjev, A.. (2016). Developmental instability in German Iris flower as a potential biomonitoring method. in Archives of Biological Sciences, 68(4), 837-844.
https://doi.org/10.2298/ABS151229072B

Barišić-Klisarić N, Miljković D, Avramov S, Živković U, Tarasjev A. Developmental instability in German Iris flower as a potential biomonitoring method. in Archives of Biological Sciences. 2016;68(4):837-844.
doi:10.2298/ABS151229072B .

Barišić-Klisarić, Nataša, Miljković, Danijela, Avramov, Stevan, Živković, Uroš, Tarasjev, Aleksej, "Developmental instability in German Iris flower as a potential biomonitoring method" in Archives of Biological Sciences, 68, no. 4 (2016):837-844,
https://doi.org/10.2298/ABS151229072B . .

TY  - JOUR
AU  - Živković, Uroš
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Tarasjev, Aleksej
AU  - Avramov, Stevan
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2036
AB  - The amount and pattern of individual phenotypic responses to seasonal
   changes in environmental conditions were determined in clones of Iris
   variegata growing in differing light habitats. For the purpose of the
   study, 97 clonal plants of the rhizomatous herb I. variegata that
   experienced different light conditions in their two native habitats were
   selected: one along the top and slope of sand dunes and one in woodland
   understories. Two fully expanded leaves that had developed during
   spring, summer and fall in two consecutive years were sampled from each
   of these clones. Six leaf traits affecting the photosynthetic rate of a
   plant -morphological (specific leaf area), anatomical (stomatal density)
   and physiological (total chlorophyll concentration, chlorophyll
   a/chlorophyll b ratio, carotenoid concentration, chlorophyll
   a/carotenoid ratio) exhibited significant plastic responses in the two
   different light habitats. To test whether these traits differ between
   exposed and shaded habitats as well as during different vegetation
   periods, we used the repeated model analysis of variance (ANOVA).
   Results of the repeated ANOVA revealed statistically significant effects
   of year, habitat and period of vegetation season. Patterns of changes
   during growing seasons were year-specific for almost all analyzed
   traits.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Seasonal variation of leaf ecophysiological traits of iris variegata observed in two consecutive years in natural habitats with contrasting light conditions
IS  - 4
VL  - 67
DO  - 10.2298/ABS150311099Z
SP  - 1227
EP  - 1236
ER  - 

@article{
author = "Živković, Uroš and Miljković, Danijela and Barišić Klisarić, Nataša and Tarasjev, Aleksej and Avramov, Stevan",
year = "2015",
abstract = "The amount and pattern of individual phenotypic responses to seasonal
   changes in environmental conditions were determined in clones of Iris
   variegata growing in differing light habitats. For the purpose of the
   study, 97 clonal plants of the rhizomatous herb I. variegata that
   experienced different light conditions in their two native habitats were
   selected: one along the top and slope of sand dunes and one in woodland
   understories. Two fully expanded leaves that had developed during
   spring, summer and fall in two consecutive years were sampled from each
   of these clones. Six leaf traits affecting the photosynthetic rate of a
   plant -morphological (specific leaf area), anatomical (stomatal density)
   and physiological (total chlorophyll concentration, chlorophyll
   a/chlorophyll b ratio, carotenoid concentration, chlorophyll
   a/carotenoid ratio) exhibited significant plastic responses in the two
   different light habitats. To test whether these traits differ between
   exposed and shaded habitats as well as during different vegetation
   periods, we used the repeated model analysis of variance (ANOVA).
   Results of the repeated ANOVA revealed statistically significant effects
   of year, habitat and period of vegetation season. Patterns of changes
   during growing seasons were year-specific for almost all analyzed
   traits.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Seasonal variation of leaf ecophysiological traits of iris variegata observed in two consecutive years in natural habitats with contrasting light conditions",
number = "4",
volume = "67",
doi = "10.2298/ABS150311099Z",
pages = "1227-1236"
}

Živković, U., Miljković, D., Barišić Klisarić, N., Tarasjev, A.,& Avramov, S.. (2015). Seasonal variation of leaf ecophysiological traits of iris variegata observed in two consecutive years in natural habitats with contrasting light conditions. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 67(4), 1227-1236.
https://doi.org/10.2298/ABS150311099Z

Živković U, Miljković D, Barišić Klisarić N, Tarasjev A, Avramov S. Seasonal variation of leaf ecophysiological traits of iris variegata observed in two consecutive years in natural habitats with contrasting light conditions. in Archives of Biological Sciences. 2015;67(4):1227-1236.
doi:10.2298/ABS150311099Z .

Živković, Uroš, Miljković, Danijela, Barišić Klisarić, Nataša, Tarasjev, Aleksej, Avramov, Stevan, "Seasonal variation of leaf ecophysiological traits of iris variegata observed in two consecutive years in natural habitats with contrasting light conditions" in Archives of Biological Sciences, 67, no. 4 (2015):1227-1236,
https://doi.org/10.2298/ABS150311099Z . .

TY  - CONF
AU  - Živković, Uroš
AU  - Avramov, Stevan
AU  - Barišić Klisarić, Nataša
AU  - Miljković, Danijela
AU  - Tubić, Ljiljana
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Tarasjev, Aleksej
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6677
AB  - During their evolution plants developed a set of mechanisms to adapt to the varying environmental conditions, resulting in diversified phenotypes. We were interested in the response of plants towards changing environmental parameters, to light in particular. For this study we selected genotypes of Iris variegata that experienced different light conditions in their habitats: a) genotypes situated along the top and slope of a dune, where it coexisted with annual and perennial herbs and low shrubs; b) genotypes inhabited the woodland understories. We grew I.variegata genotypes in two experimental light treatments (high and low) and tested differences between spring, summer and autumn as well as between two successive years. The plants responses were analysed at different levels of biological information and it was revealed that dissimilar light conditions markedly affected all aspects of plant anatomy and  physiology. Repeated measures model ANOVA detected a significant effect of year, habitat and genotype in most analysed traits. Models of changes during growing seasons were treatment- and year-specific. We also present results from an on-going study which aims to assess occurrence patterns of 
phenotypic plasticity based on system-level analysis of the same sample that includes metabolic profiling approach combined with robust statistical analysis.
PB  - Université de Lausanne, Départment d’écologie et évolution
C3  - Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland
T1  - Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6677
ER  - 

@conference{
author = "Živković, Uroš and Avramov, Stevan and Barišić Klisarić, Nataša and Miljković, Danijela and Tubić, Ljiljana and Mišić, Danijela and Šiler, Branislav and Tarasjev, Aleksej",
year = "2015",
abstract = "During their evolution plants developed a set of mechanisms to adapt to the varying environmental conditions, resulting in diversified phenotypes. We were interested in the response of plants towards changing environmental parameters, to light in particular. For this study we selected genotypes of Iris variegata that experienced different light conditions in their habitats: a) genotypes situated along the top and slope of a dune, where it coexisted with annual and perennial herbs and low shrubs; b) genotypes inhabited the woodland understories. We grew I.variegata genotypes in two experimental light treatments (high and low) and tested differences between spring, summer and autumn as well as between two successive years. The plants responses were analysed at different levels of biological information and it was revealed that dissimilar light conditions markedly affected all aspects of plant anatomy and  physiology. Repeated measures model ANOVA detected a significant effect of year, habitat and genotype in most analysed traits. Models of changes during growing seasons were treatment- and year-specific. We also present results from an on-going study which aims to assess occurrence patterns of 
phenotypic plasticity based on system-level analysis of the same sample that includes metabolic profiling approach combined with robust statistical analysis.",
publisher = "Université de Lausanne, Départment d’écologie et évolution",
journal = "Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland",
title = "Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6677"
}

Živković, U., Avramov, S., Barišić Klisarić, N., Miljković, D., Tubić, L., Mišić, D., Šiler, B.,& Tarasjev, A.. (2015). Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions. in Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland
Université de Lausanne, Départment d’écologie et évolution..
https://hdl.handle.net/21.15107/rcub_ibiss_6677

Živković U, Avramov S, Barišić Klisarić N, Miljković D, Tubić L, Mišić D, Šiler B, Tarasjev A. Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions. in Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland. 2015;.
https://hdl.handle.net/21.15107/rcub_ibiss_6677 .

Živković, Uroš, Avramov, Stevan, Barišić Klisarić, Nataša, Miljković, Danijela, Tubić, Ljiljana, Mišić, Danijela, Šiler, Branislav, Tarasjev, Aleksej, "Annual and seasonal variability in leaf traits of Iris variegata genotypes growing in contrasting light conditions" in Congress Program and Information: Congress of the European Society for Evolutionary biology: ESEB 2015; 2015 Aug 10-14; Laussane, Switzerland (2015),
https://hdl.handle.net/21.15107/rcub_ibiss_6677 .

TY  - CONF
AU  - Tarasjev, Aleksej
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Živković, Uroš
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6658
AB  - Population scale multi-year monitoring of flowering and fruiting of Iris pumila L. can shed light on various
evolutionary issues such as mechanisms of flower color polymorphism maintenance as well as provide
information on population structure and dynamics that is important for this species in situ conservation. In the monitoring process flowering is recorded each year in 35 experimental plots (more than 4000 m2 in total) on two-day basis during flowering period (early spring) in population occupying undisturbed natural habitat in the Natural Protected Reserve of Deliblato Sands. That enables determination of spatial position, flowering date, flowering span, flower color, and pollination success for more than thirteen thousand individual flowering ramets per year. Preliminary findings indicate that in the second year of monitoring flowering started almost a month earlier and had almost two times greater flowering span compared to the first year. Number of flowering individual ramets was lower, but fruit to flower ratio was by the order of magnitude higher in the second year of the study.
PB  - Beograd: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Population scale multi-year monitoring of Iris pumila in Deliblato Sand: flowering phenology
SP  - 131
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6658
ER  - 

@conference{
author = "Tarasjev, Aleksej and Avramov, Stevan and Miljković, Danijela and Barišić Klisarić, Nataša and Živković, Uroš",
year = "2015",
abstract = "Population scale multi-year monitoring of flowering and fruiting of Iris pumila L. can shed light on various
evolutionary issues such as mechanisms of flower color polymorphism maintenance as well as provide
information on population structure and dynamics that is important for this species in situ conservation. In the monitoring process flowering is recorded each year in 35 experimental plots (more than 4000 m2 in total) on two-day basis during flowering period (early spring) in population occupying undisturbed natural habitat in the Natural Protected Reserve of Deliblato Sands. That enables determination of spatial position, flowering date, flowering span, flower color, and pollination success for more than thirteen thousand individual flowering ramets per year. Preliminary findings indicate that in the second year of monitoring flowering started almost a month earlier and had almost two times greater flowering span compared to the first year. Number of flowering individual ramets was lower, but fruit to flower ratio was by the order of magnitude higher in the second year of the study.",
publisher = "Beograd: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Population scale multi-year monitoring of Iris pumila in Deliblato Sand: flowering phenology",
pages = "131",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6658"
}

Tarasjev, A., Avramov, S., Miljković, D., Barišić Klisarić, N.,& Živković, U.. (2015). Population scale multi-year monitoring of Iris pumila in Deliblato Sand: flowering phenology. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Beograd: Serbian Plant Physiology Society., 131.
https://hdl.handle.net/21.15107/rcub_ibiss_6658

Tarasjev A, Avramov S, Miljković D, Barišić Klisarić N, Živković U. Population scale multi-year monitoring of Iris pumila in Deliblato Sand: flowering phenology. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:131.
https://hdl.handle.net/21.15107/rcub_ibiss_6658 .

Tarasjev, Aleksej, Avramov, Stevan, Miljković, Danijela, Barišić Klisarić, Nataša, Živković, Uroš, "Population scale multi-year monitoring of Iris pumila in Deliblato Sand: flowering phenology" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):131,
https://hdl.handle.net/21.15107/rcub_ibiss_6658 .

TY  - CONF
AU  - Vujić, Vukica
AU  - Avramov, Stevan
AU  - Barišić Klisarić, Nataša
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
AU  - Miljković, Danijela
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6657
AB  - We employed the technique of geometric morphometrics to study variation of geometric size and shape
of Iris pumila flower parts (standard, fall and style) from plants growing in two natural habitats (exposed and shaded) in a protected natural reserve of the Deliblato Sands. We applied analysis of variance (ANOVA) for centroid size (CS) and multivariate analysis of variance (MANOVA) with all shape variables (Procrustes coordinates) as dependent variables, and with habitat as fixed and clone as a random factor. Landmarks and semilandmarks of standards, falls and styles were positioned in MakeFan6 and TpsDig program. The CVA (Canonical discriminant analysis) was used to visualize the differences of shape between the habitats with contrasting ambient light conditions. The heterogeneity of light conditions affected the flower shape. The mean values of centroid size (as a measure of geometric size) of I. pumila standards and falls were lower in open habitat than in shaded environment. The results of ANOVA revealed a statistically significant effect of habitat for standards, fall and style (p = 0.0009, p = 0.0223 and p = 0.0358, respectively,) and between-clone variability in size of all flower parts was also observed (all p < 0.0001). According to the results of MANOVA analysis significant differences in shape between habitats and clones (all p < 0.0001) were observed. Considering that the light intensity is significantly different in open and shaded habitats, we can only assume that this is the key factor that influences the size and shape variation of I. pumila floral parts.
PB  - Beograd: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Light induces variation in size and shape of Iris pumila flower parts in two natural habitats
SP  - 131
EP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6657
ER  - 

@conference{
author = "Vujić, Vukica and Avramov, Stevan and Barišić Klisarić, Nataša and Živković, Uroš and Tarasjev, Aleksej and Miljković, Danijela",
year = "2015",
abstract = "We employed the technique of geometric morphometrics to study variation of geometric size and shape
of Iris pumila flower parts (standard, fall and style) from plants growing in two natural habitats (exposed and shaded) in a protected natural reserve of the Deliblato Sands. We applied analysis of variance (ANOVA) for centroid size (CS) and multivariate analysis of variance (MANOVA) with all shape variables (Procrustes coordinates) as dependent variables, and with habitat as fixed and clone as a random factor. Landmarks and semilandmarks of standards, falls and styles were positioned in MakeFan6 and TpsDig program. The CVA (Canonical discriminant analysis) was used to visualize the differences of shape between the habitats with contrasting ambient light conditions. The heterogeneity of light conditions affected the flower shape. The mean values of centroid size (as a measure of geometric size) of I. pumila standards and falls were lower in open habitat than in shaded environment. The results of ANOVA revealed a statistically significant effect of habitat for standards, fall and style (p = 0.0009, p = 0.0223 and p = 0.0358, respectively,) and between-clone variability in size of all flower parts was also observed (all p < 0.0001). According to the results of MANOVA analysis significant differences in shape between habitats and clones (all p < 0.0001) were observed. Considering that the light intensity is significantly different in open and shaded habitats, we can only assume that this is the key factor that influences the size and shape variation of I. pumila floral parts.",
publisher = "Beograd: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Light induces variation in size and shape of Iris pumila flower parts in two natural habitats",
pages = "131-132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6657"
}

Vujić, V., Avramov, S., Barišić Klisarić, N., Živković, U., Tarasjev, A.,& Miljković, D.. (2015). Light induces variation in size and shape of Iris pumila flower parts in two natural habitats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Beograd: Serbian Plant Physiology Society., 131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6657

Vujić V, Avramov S, Barišić Klisarić N, Živković U, Tarasjev A, Miljković D. Light induces variation in size and shape of Iris pumila flower parts in two natural habitats. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:131-132.
https://hdl.handle.net/21.15107/rcub_ibiss_6657 .

Vujić, Vukica, Avramov, Stevan, Barišić Klisarić, Nataša, Živković, Uroš, Tarasjev, Aleksej, Miljković, Danijela, "Light induces variation in size and shape of Iris pumila flower parts in two natural habitats" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):131-132,
https://hdl.handle.net/21.15107/rcub_ibiss_6657 .

TY  - CONF
AU  - Živković, Uroš
AU  - Miljković, Danijela
AU  - Barišić Klisarić, Nataša
AU  - Tarasjev, Aleksej
AU  - Avramov, Stevan
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6656
AB  - Plants grown under canopy shade show a range of responses to the change in light quantity as well as
in red/far-red ratio of the ambient light, i.e. light quality. These responses, known as the shade-avoidance syndrome, or near neighbor detection response, are characterized, among others, by changes in flowering phenology and reproductive output. The individual’s flowering pattern is defined by the timing or temporal distribution of flowering (start of flowering, average flowering period), the duration of flowering, as well as the number of flowers produced. We analyzed the genetic variability and phenotypic plasticity of flowering pattern and reproductive success in 97 genotypes of Iris variegata originating from contrasting light habitats in Deliblato Sands and expressed under different experimental light conditions. Rhizome segments were taken from each of these clones and transplanted in the experimental garden near the Institute for Biological Research in Belgrade. Significant differences between genotypes were found for two traits (start of flowering, average flowering time). Genotypes originating from open and understory habitats significantly differed in three traits (number of flowers, number of capsules/number of flowers, seed mass/capsule). Significant effect of light treatment was found for three traits (number of capsules/number of flowers, seed mass/capsule, average seed mass). Statistically significant correlations between explored traits were generally similar but also to some extent habitat- and treatment-specific. Comparing these results with research carried out on congeneric
species we noted that there were similar responses for some traits, but also significant differences in
some components of flowering and fruiting success.
PB  - Beograd: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Flowering and reproductive performance of Iris variegata genotypes in different light conditions
SP  - 139
EP  - 140
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6656
ER  - 

@conference{
author = "Živković, Uroš and Miljković, Danijela and Barišić Klisarić, Nataša and Tarasjev, Aleksej and Avramov, Stevan",
year = "2015",
abstract = "Plants grown under canopy shade show a range of responses to the change in light quantity as well as
in red/far-red ratio of the ambient light, i.e. light quality. These responses, known as the shade-avoidance syndrome, or near neighbor detection response, are characterized, among others, by changes in flowering phenology and reproductive output. The individual’s flowering pattern is defined by the timing or temporal distribution of flowering (start of flowering, average flowering period), the duration of flowering, as well as the number of flowers produced. We analyzed the genetic variability and phenotypic plasticity of flowering pattern and reproductive success in 97 genotypes of Iris variegata originating from contrasting light habitats in Deliblato Sands and expressed under different experimental light conditions. Rhizome segments were taken from each of these clones and transplanted in the experimental garden near the Institute for Biological Research in Belgrade. Significant differences between genotypes were found for two traits (start of flowering, average flowering time). Genotypes originating from open and understory habitats significantly differed in three traits (number of flowers, number of capsules/number of flowers, seed mass/capsule). Significant effect of light treatment was found for three traits (number of capsules/number of flowers, seed mass/capsule, average seed mass). Statistically significant correlations between explored traits were generally similar but also to some extent habitat- and treatment-specific. Comparing these results with research carried out on congeneric
species we noted that there were similar responses for some traits, but also significant differences in
some components of flowering and fruiting success.",
publisher = "Beograd: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Flowering and reproductive performance of Iris variegata genotypes in different light conditions",
pages = "139-140",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6656"
}

Živković, U., Miljković, D., Barišić Klisarić, N., Tarasjev, A.,& Avramov, S.. (2015). Flowering and reproductive performance of Iris variegata genotypes in different light conditions. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Beograd: Serbian Plant Physiology Society., 139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6656

Živković U, Miljković D, Barišić Klisarić N, Tarasjev A, Avramov S. Flowering and reproductive performance of Iris variegata genotypes in different light conditions. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:139-140.
https://hdl.handle.net/21.15107/rcub_ibiss_6656 .

Živković, Uroš, Miljković, Danijela, Barišić Klisarić, Nataša, Tarasjev, Aleksej, Avramov, Stevan, "Flowering and reproductive performance of Iris variegata genotypes in different light conditions" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):139-140,
https://hdl.handle.net/21.15107/rcub_ibiss_6656 .

TY  - JOUR
AU  - Vujić, Vukica
AU  - Avramov, Stevan
AU  - Tarasjev, Aleksej
AU  - Barišić Klisarić, Nataša
AU  - Živković, Uroš
AU  - Miljković, Danijela
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6652
AB  - The function of flowers, as the reproductive organs of plants, can be altered by changes in the size and shape of their constituent parts in traffic-related air polluted areas. The aim of this work was to compare the length, width, centroid size and shape (using a set of shape variables - partal warps) of Iris pumila standards (petals) from an urban area of Belgrade city with data from the Deliblato Sands – an unpolluted nature reserve. Differences between the areas, as well as differences in clonal variability, for length, centroid size (ANOVA p < 0.001) and shape variation of standards were significant (MANOVA p < 0.001). Micro-environmental conditions induced significant diversity between flowers within a clone (p < 0.001), while intra-individual variation (part effects, p > 0.05) was not confirmed. Decrease of standard length and centroid size in the polluted environment was detected, as well as a change in the shape of this I. pumila flower part. The standards were shorter and wider in the polluted area when compared to those in the unpolluted environment.
PB  - Budapest: Corvinus University
T2  - Applied Ecology and Environmental Research
T1  - The effects of traffic-related air pollution on the flower morphology of Iris pumila – Comparison of a polluted city area and the unpolluted Deliblato sands (Nature reserve)
IS  - 2
VL  - 13
DO  - 10.15666/aeer/1302_405415
SP  - 405
EP  - 415
ER  - 

@article{
author = "Vujić, Vukica and Avramov, Stevan and Tarasjev, Aleksej and Barišić Klisarić, Nataša and Živković, Uroš and Miljković, Danijela",
year = "2015",
abstract = "The function of flowers, as the reproductive organs of plants, can be altered by changes in the size and shape of their constituent parts in traffic-related air polluted areas. The aim of this work was to compare the length, width, centroid size and shape (using a set of shape variables - partal warps) of Iris pumila standards (petals) from an urban area of Belgrade city with data from the Deliblato Sands – an unpolluted nature reserve. Differences between the areas, as well as differences in clonal variability, for length, centroid size (ANOVA p < 0.001) and shape variation of standards were significant (MANOVA p < 0.001). Micro-environmental conditions induced significant diversity between flowers within a clone (p < 0.001), while intra-individual variation (part effects, p > 0.05) was not confirmed. Decrease of standard length and centroid size in the polluted environment was detected, as well as a change in the shape of this I. pumila flower part. The standards were shorter and wider in the polluted area when compared to those in the unpolluted environment.",
publisher = "Budapest: Corvinus University",
journal = "Applied Ecology and Environmental Research",
title = "The effects of traffic-related air pollution on the flower morphology of Iris pumila – Comparison of a polluted city area and the unpolluted Deliblato sands (Nature reserve)",
number = "2",
volume = "13",
doi = "10.15666/aeer/1302_405415",
pages = "405-415"
}

Vujić, V., Avramov, S., Tarasjev, A., Barišić Klisarić, N., Živković, U.,& Miljković, D.. (2015). The effects of traffic-related air pollution on the flower morphology of Iris pumila – Comparison of a polluted city area and the unpolluted Deliblato sands (Nature reserve). in Applied Ecology and Environmental Research
Budapest: Corvinus University., 13(2), 405-415.
https://doi.org/10.15666/aeer/1302_405415

Vujić V, Avramov S, Tarasjev A, Barišić Klisarić N, Živković U, Miljković D. The effects of traffic-related air pollution on the flower morphology of Iris pumila – Comparison of a polluted city area and the unpolluted Deliblato sands (Nature reserve). in Applied Ecology and Environmental Research. 2015;13(2):405-415.
doi:10.15666/aeer/1302_405415 .

Vujić, Vukica, Avramov, Stevan, Tarasjev, Aleksej, Barišić Klisarić, Nataša, Živković, Uroš, Miljković, Danijela, "The effects of traffic-related air pollution on the flower morphology of Iris pumila – Comparison of a polluted city area and the unpolluted Deliblato sands (Nature reserve)" in Applied Ecology and Environmental Research, 13, no. 2 (2015):405-415,
https://doi.org/10.15666/aeer/1302_405415 . .

TY  - CONF
AU  - Barišić Klisarić, Nataša
AU  - Avramov, Stevan
AU  - Miljković, Danijela
AU  - Živković, Uroš
AU  - Tarasjev, Aleksej
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5452
AB  - Plants as sessile organisms have to continuously adjust their growth, development and reproduction to
variable surroundings, especially in relation to light as an essential abiotic factor. Plants growing in crowded
conditions inevitably compete for light, namely, for an ability to photosynthesize. In this study, we addressed
correlation structures between traits essential for the response to changes in light quality and light intensity, in Lamium purpureum plants originating from two habitats (Open and Shade), in two natural populations
(Vršac mountain and Zuce) and grown in two densities (High and Low). Every single plant was planted in a
single pot and this arrangement allowed competition only for light and not for the other resources. We measured
eight traits after 8 weeks of growth: height, first and second leaf length, first and second leaf width and
number of leaves, number of internodes and number of shoots per plant. We aimed to test if there are statistically
significant phenotypic correlations between traits (morphological integration) and if they depend
on the population, habitat and density treatment. Correlation structures differed between habitats and treatments
in both populations. Plants in Vrsac/Shade habitat and Zuce/Open habitat, showed the lowest level
of between trait integration in the High light treatment. Between-population differences in correlation structures
were more numerous in the High light treatment for both habitats.
PB  - Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Morphological integration of vegetative traits in Lamium purpureum from two habitats (Open and Shade), in two natural populations (Vršac mountain and Avala mountain) and grown in two densities (High and Low).
SP  - 133
EP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5452
ER  - 

@conference{
author = "Barišić Klisarić, Nataša and Avramov, Stevan and Miljković, Danijela and Živković, Uroš and Tarasjev, Aleksej",
year = "2015",
abstract = "Plants as sessile organisms have to continuously adjust their growth, development and reproduction to
variable surroundings, especially in relation to light as an essential abiotic factor. Plants growing in crowded
conditions inevitably compete for light, namely, for an ability to photosynthesize. In this study, we addressed
correlation structures between traits essential for the response to changes in light quality and light intensity, in Lamium purpureum plants originating from two habitats (Open and Shade), in two natural populations
(Vršac mountain and Zuce) and grown in two densities (High and Low). Every single plant was planted in a
single pot and this arrangement allowed competition only for light and not for the other resources. We measured
eight traits after 8 weeks of growth: height, first and second leaf length, first and second leaf width and
number of leaves, number of internodes and number of shoots per plant. We aimed to test if there are statistically
significant phenotypic correlations between traits (morphological integration) and if they depend
on the population, habitat and density treatment. Correlation structures differed between habitats and treatments
in both populations. Plants in Vrsac/Shade habitat and Zuce/Open habitat, showed the lowest level
of between trait integration in the High light treatment. Between-population differences in correlation structures
were more numerous in the High light treatment for both habitats.",
publisher = "Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Morphological integration of vegetative traits in Lamium purpureum from two habitats (Open and Shade), in two natural populations (Vršac mountain and Avala mountain) and grown in two densities (High and Low).",
pages = "133-134",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5452"
}

Barišić Klisarić, N., Avramov, S., Miljković, D., Živković, U.,& Tarasjev, A.. (2015). Morphological integration of vegetative traits in Lamium purpureum from two habitats (Open and Shade), in two natural populations (Vršac mountain and Avala mountain) and grown in two densities (High and Low).. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society: Institute for Biological Research “Siniša Stanković”., 133-134.
https://hdl.handle.net/21.15107/rcub_ibiss_5452

Barišić Klisarić N, Avramov S, Miljković D, Živković U, Tarasjev A. Morphological integration of vegetative traits in Lamium purpureum from two habitats (Open and Shade), in two natural populations (Vršac mountain and Avala mountain) and grown in two densities (High and Low).. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:133-134.
https://hdl.handle.net/21.15107/rcub_ibiss_5452 .

Barišić Klisarić, Nataša, Avramov, Stevan, Miljković, Danijela, Živković, Uroš, Tarasjev, Aleksej, "Morphological integration of vegetative traits in Lamium purpureum from two habitats (Open and Shade), in two natural populations (Vršac mountain and Avala mountain) and grown in two densities (High and Low)." in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):133-134,
https://hdl.handle.net/21.15107/rcub_ibiss_5452 .