TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Pavlović, Slađan
AU  - Dinčić, Evica
AU  - Kuveljić, Jovana
AU  - Manojlović, Dragan
AU  - Živković, Maja
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6584
AB  - Background: Multiple sclerosis (MS) is a chronic demyelinating disorder intricately linked to perturbations in
trace element levels. While previous studies have explored circulating trace elements in a limited sample, understanding the impact of demographic and clinical variables on the elemental profile within a larger cohort
remains elusive.
Methods: This study aimed to evaluate essential trace elements (Cr, Mn, Co, Cu, Zn, and Se) in the sera of 215 MS
patients compared to a meticulously matched control group of 100 individuals with similar gender and age. Our
main objective was to identify potential variations in elemental profiles based on demographic and clinical
parameters among MS patients, elucidating the prospective relevance of supplementing specific essential trace
elements.
Results: Data indicated a significant decrease in serum levels of Mn, Co, Zn, and Se, and an increase in Cr in MS
patients compared to controls. These trace elements not only discriminated between MS patients and controls but
also exhibited distinctive capabilities among demographic subgroups. Gender, smoking habits, and age strata
(20–40 years and 41–60 years) revealed discernible variations in elemental profiles between MS patients and
their control counterparts. Se demonstrated the singular ability to stratify cases of extreme MS severity, mild
relapsing-remitting MS (RRMS) and highly severe secondary progressive MS (SPMS). In contrast, Co significantly
differentiated RRMS from primary progressive MS (PPMS), while Cu significantly differentiated SPMS from
PPMS. Additionally, Cu showed a negative correlation with MSSS, while Mn and Zn showed a positive correlation
with EDSS.
Conclusion: These findings underscore a substantive deficiency in Mn, Co, Zn, and Se in the MS cohort, supporting
targeted supplementation with these trace elements. This study provides a comprehensive understanding of the
intricate relationship between essential trace elements and MS, paving the way for further research into
personalized nutritional interventions for this complex neurological disorder.
PB  - Elsevier
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation
VL  - 83
DO  - 10.1016/j.jtemb.2024.127421
SP  - 127421
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Jagodić, Jovana and Pavlović, Slađan and Dinčić, Evica and Kuveljić, Jovana and Manojlović, Dragan and Živković, Maja",
year = "2024",
abstract = "Background: Multiple sclerosis (MS) is a chronic demyelinating disorder intricately linked to perturbations in
trace element levels. While previous studies have explored circulating trace elements in a limited sample, understanding the impact of demographic and clinical variables on the elemental profile within a larger cohort
remains elusive.
Methods: This study aimed to evaluate essential trace elements (Cr, Mn, Co, Cu, Zn, and Se) in the sera of 215 MS
patients compared to a meticulously matched control group of 100 individuals with similar gender and age. Our
main objective was to identify potential variations in elemental profiles based on demographic and clinical
parameters among MS patients, elucidating the prospective relevance of supplementing specific essential trace
elements.
Results: Data indicated a significant decrease in serum levels of Mn, Co, Zn, and Se, and an increase in Cr in MS
patients compared to controls. These trace elements not only discriminated between MS patients and controls but
also exhibited distinctive capabilities among demographic subgroups. Gender, smoking habits, and age strata
(20–40 years and 41–60 years) revealed discernible variations in elemental profiles between MS patients and
their control counterparts. Se demonstrated the singular ability to stratify cases of extreme MS severity, mild
relapsing-remitting MS (RRMS) and highly severe secondary progressive MS (SPMS). In contrast, Co significantly
differentiated RRMS from primary progressive MS (PPMS), while Cu significantly differentiated SPMS from
PPMS. Additionally, Cu showed a negative correlation with MSSS, while Mn and Zn showed a positive correlation
with EDSS.
Conclusion: These findings underscore a substantive deficiency in Mn, Co, Zn, and Se in the MS cohort, supporting
targeted supplementation with these trace elements. This study provides a comprehensive understanding of the
intricate relationship between essential trace elements and MS, paving the way for further research into
personalized nutritional interventions for this complex neurological disorder.",
publisher = "Elsevier",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation",
volume = "83",
doi = "10.1016/j.jtemb.2024.127421",
pages = "127421"
}

Stojsavljević, A., Jagodić, J., Pavlović, S., Dinčić, E., Kuveljić, J., Manojlović, D.,& Živković, M.. (2024). Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation. in Journal of Trace Elements in Medicine and Biology
Elsevier., 83, 127421.
https://doi.org/10.1016/j.jtemb.2024.127421

Stojsavljević A, Jagodić J, Pavlović S, Dinčić E, Kuveljić J, Manojlović D, Živković M. Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation. in Journal of Trace Elements in Medicine and Biology. 2024;83:127421.
doi:10.1016/j.jtemb.2024.127421 .

Stojsavljević, Aleksandar, Jagodić, Jovana, Pavlović, Slađan, Dinčić, Evica, Kuveljić, Jovana, Manojlović, Dragan, Živković, Maja, "Essential Trace Element Levels in Multiple Sclerosis: Bridging Demographic and Clinical Gaps, Assessing the Need for Supplementation" in Journal of Trace Elements in Medicine and Biology, 83 (2024):127421,
https://doi.org/10.1016/j.jtemb.2024.127421 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Lakićević, Novak
AU  - Pavlović, Slađan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6438
AB  - Mercury (Hg) is a non-essential trace metal with unique neurochemical properties and
harmful effects on the central nervous system. In this study, we present a comprehensive review
and meta-analysis of peer-reviewed research encompassing five crucial clinical matrices: hair, whole
blood, plasma, red blood cells (RBCs), and urine. We assess the disparities in Hg levels between
gender- and age-matched neurotypical children (controls) and children diagnosed with autism spectrum disorder (ASD) (cases). After applying rigorous selection criteria, we incorporated a total of
60 case-control studies into our meta-analysis. These studies comprised 25 investigations of Hg levels
in hair (controls/cases: 1134/1361), 15 in whole blood (controls/cases: 1019/1345), 6 in plasma (controls/cases: 224/263), 5 in RBCs (controls/cases: 215/293), and 9 in urine (controls/cases: 399/623).
This meta-analysis did not include the data of ASD children who received chelation therapy. Our
meta-analysis revealed no statistically significant differences in Hg levels in hair and urine between
ASD cases and controls. In whole blood, plasma, and RBCs, Hg levels were significantly higher
in ASD cases compared to their neurotypical counterparts. This indicates that ASD children could
exhibit reduced detoxification capacity for Hg and impaired mechanisms for Hg excretion from their
bodies. This underscores the detrimental role of Hg in ASD and underscores the critical importance of
monitoring Hg levels in ASD children, particularly in early childhood. These findings emphasize the
pressing need for global initiatives aimed at minimizing Hg exposure, thus highlighting the critical
intersection of human–environment interaction and neurodevelopment health.
PB  - Basel: MDPI
T2  - Biomedicines
T1  - Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis
IS  - 3344
IS  - 12
VL  - 11
DO  - 10.3390/biomedicines11123344
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Lakićević, Novak and Pavlović, Slađan",
year = "2023",
abstract = "Mercury (Hg) is a non-essential trace metal with unique neurochemical properties and
harmful effects on the central nervous system. In this study, we present a comprehensive review
and meta-analysis of peer-reviewed research encompassing five crucial clinical matrices: hair, whole
blood, plasma, red blood cells (RBCs), and urine. We assess the disparities in Hg levels between
gender- and age-matched neurotypical children (controls) and children diagnosed with autism spectrum disorder (ASD) (cases). After applying rigorous selection criteria, we incorporated a total of
60 case-control studies into our meta-analysis. These studies comprised 25 investigations of Hg levels
in hair (controls/cases: 1134/1361), 15 in whole blood (controls/cases: 1019/1345), 6 in plasma (controls/cases: 224/263), 5 in RBCs (controls/cases: 215/293), and 9 in urine (controls/cases: 399/623).
This meta-analysis did not include the data of ASD children who received chelation therapy. Our
meta-analysis revealed no statistically significant differences in Hg levels in hair and urine between
ASD cases and controls. In whole blood, plasma, and RBCs, Hg levels were significantly higher
in ASD cases compared to their neurotypical counterparts. This indicates that ASD children could
exhibit reduced detoxification capacity for Hg and impaired mechanisms for Hg excretion from their
bodies. This underscores the detrimental role of Hg in ASD and underscores the critical importance of
monitoring Hg levels in ASD children, particularly in early childhood. These findings emphasize the
pressing need for global initiatives aimed at minimizing Hg exposure, thus highlighting the critical
intersection of human–environment interaction and neurodevelopment health.",
publisher = "Basel: MDPI",
journal = "Biomedicines",
title = "Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis",
number = "3344, 12",
volume = "11",
doi = "10.3390/biomedicines11123344"
}

Stojsavljević, A., Lakićević, N.,& Pavlović, S.. (2023). Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis. in Biomedicines
Basel: MDPI., 11(3344).
https://doi.org/10.3390/biomedicines11123344

Stojsavljević A, Lakićević N, Pavlović S. Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis. in Biomedicines. 2023;11(3344).
doi:10.3390/biomedicines11123344 .

Stojsavljević, Aleksandar, Lakićević, Novak, Pavlović, Slađan, "Mercury and Autism Spectrum Disorder: Exploring the Link through Comprehensive Review and Meta-Analysis" in Biomedicines, 11, no. 3344 (2023),
https://doi.org/10.3390/biomedicines11123344 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Lakićević, Novak
AU  - Pavlović, Slađan
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6122
AB  - Environmental pollutants, particularly toxic trace metals with neurotoxic potential, have
been related to the genesis of autism. One of these metals that stands out, in particular, is lead (Pb).
We conducted an in-depth systematic review and meta-analysis of peer-reviewed studies on Pb levels
in biological materials retrieved from autistic children (cases) and neurotypical children (controls)
in this work. A systematic review was conducted after the careful selection of published studies
according to established criteria to gain a broad insight into the higher or lower levels of Pb in the
biological materials of cases and controls, and the findings were then strengthened by a meta-analysis.
The meta-analysis included 17 studies (hair), 13 studies (whole blood), and 8 studies (urine). The
overall number of controls/cases was 869/915 (hair), 670/755 (whole blood), and 344/373 (urine).
This meta-analysis showed significantly higher Pb levels in all three types of biological material in
cases than in controls, suggesting a higher body Pb burden in autistic children. Thus, environmental
Pb exposure could be related to the genesis of autism. Since no level of Pb can be considered safe,
the data from this study undoubtedly point to the importance of regularly monitoring Pb levels in
autistic children.
PB  - Basel: MDPI
T2  - Toxics
T1  - Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis
IS  - 9
VL  - 11
DO  - 10.3390/toxics11090753
SP  - 753
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Lakićević, Novak and Pavlović, Slađan",
year = "2023",
abstract = "Environmental pollutants, particularly toxic trace metals with neurotoxic potential, have
been related to the genesis of autism. One of these metals that stands out, in particular, is lead (Pb).
We conducted an in-depth systematic review and meta-analysis of peer-reviewed studies on Pb levels
in biological materials retrieved from autistic children (cases) and neurotypical children (controls)
in this work. A systematic review was conducted after the careful selection of published studies
according to established criteria to gain a broad insight into the higher or lower levels of Pb in the
biological materials of cases and controls, and the findings were then strengthened by a meta-analysis.
The meta-analysis included 17 studies (hair), 13 studies (whole blood), and 8 studies (urine). The
overall number of controls/cases was 869/915 (hair), 670/755 (whole blood), and 344/373 (urine).
This meta-analysis showed significantly higher Pb levels in all three types of biological material in
cases than in controls, suggesting a higher body Pb burden in autistic children. Thus, environmental
Pb exposure could be related to the genesis of autism. Since no level of Pb can be considered safe,
the data from this study undoubtedly point to the importance of regularly monitoring Pb levels in
autistic children.",
publisher = "Basel: MDPI",
journal = "Toxics",
title = "Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis",
number = "9",
volume = "11",
doi = "10.3390/toxics11090753",
pages = "753"
}

Stojsavljević, A., Lakićević, N.,& Pavlović, S.. (2023). Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis. in Toxics
Basel: MDPI., 11(9), 753.
https://doi.org/10.3390/toxics11090753

Stojsavljević A, Lakićević N, Pavlović S. Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis. in Toxics. 2023;11(9):753.
doi:10.3390/toxics11090753 .

Stojsavljević, Aleksandar, Lakićević, Novak, Pavlović, Slađan, "Does Lead Have a Connection to Autism? A Systematic Review and Meta-Analysis" in Toxics, 11, no. 9 (2023):753,
https://doi.org/10.3390/toxics11090753 . .

TY  - JOUR
AU  - Manojlović-Stojanoski, Milica
AU  - Borković Mitić, Slavica
AU  - Nestorović, Nataša
AU  - Ristić, Nataša
AU  - Trifunović, Svetlana
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Stojsavljević, Aleksandar
AU  - Pavlović, Slađan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5198
AB  - The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) and inorganic sodium-selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy.  Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females  started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta
IS  - 21
VL  - 23
DO  - 10.3390/ijms232113068
SP  - 13068
ER  - 

@article{
author = "Manojlović-Stojanoski, Milica and Borković Mitić, Slavica and Nestorović, Nataša and Ristić, Nataša and Trifunović, Svetlana and Stevanović, Magdalena and Filipović, Nenad and Stojsavljević, Aleksandar and Pavlović, Slađan",
year = "2022",
abstract = "The chemical element selenium (Se) is a nonmetal that is in trace amounts indispensable for normal cellular functioning. During pregnancy a low Se status can increase the risk of oxidative stress. However, elevated concentrations of Se in the body can also cause oxidative stress. This study aimed to compare the effects of BSA-stabilized Se nanoparticles (SeNPs, Se0) and inorganic sodium-selenite (NaSe, Se+4) supplementation on the histological structure of the placenta, oxidative stress parameters and the total placental Se concentration of Wistar rats during pregnancy.  Pregnant females were randomized into four groups: (i) intact controls; (ii) controls that were dosed by daily oral gavage with 8.6% bovine serum albumin (BSA) and 0.125 M vit C; (iii) the SeNP group that was administered 0.5 mg of SeNPs stabilized with 8.6% BSA and 0.125 M vit C/kg bw/day by oral gavage dosing; (iv) the NaSe group, gavage dosed with 0.5 mg Na2SeO3/kg bw/day. The treatment of pregnant females  started on gestational day one, lasted until day 20, and on day 21 of gestation, the fetuses with the placenta were removed from the uterus. Our findings show that the mode of action of equivalent concentrations of Se in SeNPs and NaSe depended on its redox state and chemical structure. Administration of SeNPs (Se0) increased fetal lethality and induced changes in the antioxidative defense parameters in the placenta. The accumulation of Se in the placenta was highest in SeNP-treated animals. All obtained data indicate an increased bioavailability of Se in its organic nano form and Se0 redox state in comparison to its inorganic sodium selenite form and Se+4 redox state",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta",
number = "21",
volume = "23",
doi = "10.3390/ijms232113068",
pages = "13068"
}

Manojlović-Stojanoski, M., Borković Mitić, S., Nestorović, N., Ristić, N., Trifunović, S., Stevanović, M., Filipović, N., Stojsavljević, A.,& Pavlović, S.. (2022). The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta. in International Journal of Molecular Sciences
Basel: MDPI., 23(21), 13068.
https://doi.org/10.3390/ijms232113068

Manojlović-Stojanoski M, Borković Mitić S, Nestorović N, Ristić N, Trifunović S, Stevanović M, Filipović N, Stojsavljević A, Pavlović S. The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta. in International Journal of Molecular Sciences. 2022;23(21):13068.
doi:10.3390/ijms232113068 .

Manojlović-Stojanoski, Milica, Borković Mitić, Slavica, Nestorović, Nataša, Ristić, Nataša, Trifunović, Svetlana, Stevanović, Magdalena, Filipović, Nenad, Stojsavljević, Aleksandar, Pavlović, Slađan, "The effects of BSA-stabilized selenium nanoparticles and sodium selenite supplementation on the structure, oxidative stress parameters and selenium redox biology in rat placenta" in International Journal of Molecular Sciences, 23, no. 21 (2022):13068,
https://doi.org/10.3390/ijms232113068 . .

TY  - JOUR
AU  - Jagodić, Jovana
AU  - Pavlović, Slađan
AU  - Borković Mitić, Slavica
AU  - Perović, Milan
AU  - Miković, Željko
AU  - Đurđić, Slađana
AU  - Manojlović, Dragan
AU  - Stojsavljević, Aleksandar
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5020
AB  - With the ever-growing concern for human health and wellbeing, the prenatal period of
development requires special attention since fetuses can be exposed to various metals through the
mother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)
and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, and
placental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on the
potential transplacental transfer of these trace metals. Based on the obtained levels of investigated
elements in clinical samples, it was observed that all of the trace metals cross the placental barrier
and reach the fetus. Furthermore, statistical analysis revealed significant differences in levels of
toxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinical
samples. Correlation analysis highlighted As to be an element with levels that differed significantly
between all tested samples. Principal component analysis (PCA) was used to enhance these findings.
PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals in
distinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible for
the clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera was
responsible for the clustering of these samples. Overall, the findings of this study could contribute to
a better understanding of transplacental transfer of these trace metals, and shed a light on overall
levels of metal exposure in the population of healthy pregnant women and their fetuses.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy
IS  - 15
VL  - 23
DO  - 10.3390/ijms23158078
SP  - 8078
ER  - 

@article{
author = "Jagodić, Jovana and Pavlović, Slađan and Borković Mitić, Slavica and Perović, Milan and Miković, Željko and Đurđić, Slađana and Manojlović, Dragan and Stojsavljević, Aleksandar",
year = "2022",
abstract = "With the ever-growing concern for human health and wellbeing, the prenatal period of
development requires special attention since fetuses can be exposed to various metals through the
mother. Therefore, this study explored the status of selected toxic (Pb, Cd, Ni, As, Pt, Ce, Rb, Sr, U)
and essential trace metals (Mn, Co, Cu, Zn, Se) in the umbilical cord (UC) sera, maternal sera, and
placental tissue samples of 92 healthy women with normal pregnancies. A further aim focuses on the
potential transplacental transfer of these trace metals. Based on the obtained levels of investigated
elements in clinical samples, it was observed that all of the trace metals cross the placental barrier
and reach the fetus. Furthermore, statistical analysis revealed significant differences in levels of
toxic Ni, As, Cd, U, Sr, Rb, and essential Mn, Cu, and Zn between all three types of analyzed clinical
samples. Correlation analysis highlighted As to be an element with levels that differed significantly
between all tested samples. Principal component analysis (PCA) was used to enhance these findings.
PCA demonstrated that Cd, Mn, Zn, Rb, Ce, U, and Sr were the most influential trace metals in
distinguishing placenta from maternal and UC serum samples. As, Co, and Cu were responsible for
the clustering of maternal serum samples, and PCA demonstrated that the Pt level in UC sera was
responsible for the clustering of these samples. Overall, the findings of this study could contribute to
a better understanding of transplacental transfer of these trace metals, and shed a light on overall
levels of metal exposure in the population of healthy pregnant women and their fetuses.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy",
number = "15",
volume = "23",
doi = "10.3390/ijms23158078",
pages = "8078"
}

Jagodić, J., Pavlović, S., Borković Mitić, S., Perović, M., Miković, Ž., Đurđić, S., Manojlović, D.,& Stojsavljević, A.. (2022). Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences
Basel: MDPI., 23(15), 8078.
https://doi.org/10.3390/ijms23158078

Jagodić J, Pavlović S, Borković Mitić S, Perović M, Miković Ž, Đurđić S, Manojlović D, Stojsavljević A. Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy. in International Journal of Molecular Sciences. 2022;23(15):8078.
doi:10.3390/ijms23158078 .

Jagodić, Jovana, Pavlović, Slađan, Borković Mitić, Slavica, Perović, Milan, Miković, Željko, Đurđić, Slađana, Manojlović, Dragan, Stojsavljević, Aleksandar, "Examination of Trace Metals and Their Potential Transplacental Transfer in Pregnancy" in International Journal of Molecular Sciences, 23, no. 15 (2022):8078,
https://doi.org/10.3390/ijms23158078 . .

TY  - JOUR
AU  - Mitić, Bojan
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Sojanović, Dalibor
AU  - Pavlović, Slađan
AU  - Vasiljević, Ljubica
AU  - Ristić, Nataša
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5044
AB  - Three centipede species (Clinopodes flavidus,Cryptops anomalans and Eupolybothrus transsylvanicus) were used as bioindicators of trace metal and metalloid pollution in Belgrade, Serbia. The concentrations of 13 elements (the metals Mn, Co, Ni, Cu, Zn, Rb,Sr,Cd, Tl, Pb and U and metalloids As,Se) in whole animals and soil were measured by inductively coupled plasma mass spectrometry (ICP-MS). Differences in the concentrations of some elements in the analyzed species were observed, both in response to the sites and between species. 1n most cases, the trace element concentrations were higher in centipedes from a polluted site (an industrial area near a busy street with heavy traffic) but C. anomalans and E. transsylvanicus had higher Mn concentrations at an unpolluted site (a deciduous woodland on Mt. Avala). C. flavidus was a good bioindicator for detecting differences between Zn, Se and Cd. C. flavidus and C. anomalans were more efficient in accumulating Zn than E. transsylvanicus. It appears that C. anomalans poorly accumulated Cd, unlike C. flavidus and E. transsylvanicus, which accumulated Cd according to the high bioaccwnulation factor (BAF) values. We conclude that the centipedes C. flavidus, C. anomalans and E. transsylvanicus can be used as suitable bioindicators of trace element exposure. Their ability to accumulate trace elements was different and depends on their physiology and lifestyle as well as the route of exposure.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Metal and Metalloid bioaccumulation in three centipedes (Chilopoda)
IS  - 3
VL  - 74
DO  - 10.2298/ABS220514019M
SP  - 207
EP  - 215
ER  - 

@article{
author = "Mitić, Bojan and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Sojanović, Dalibor and Pavlović, Slađan and Vasiljević, Ljubica and Ristić, Nataša",
year = "2022",
abstract = "Three centipede species (Clinopodes flavidus,Cryptops anomalans and Eupolybothrus transsylvanicus) were used as bioindicators of trace metal and metalloid pollution in Belgrade, Serbia. The concentrations of 13 elements (the metals Mn, Co, Ni, Cu, Zn, Rb,Sr,Cd, Tl, Pb and U and metalloids As,Se) in whole animals and soil were measured by inductively coupled plasma mass spectrometry (ICP-MS). Differences in the concentrations of some elements in the analyzed species were observed, both in response to the sites and between species. 1n most cases, the trace element concentrations were higher in centipedes from a polluted site (an industrial area near a busy street with heavy traffic) but C. anomalans and E. transsylvanicus had higher Mn concentrations at an unpolluted site (a deciduous woodland on Mt. Avala). C. flavidus was a good bioindicator for detecting differences between Zn, Se and Cd. C. flavidus and C. anomalans were more efficient in accumulating Zn than E. transsylvanicus. It appears that C. anomalans poorly accumulated Cd, unlike C. flavidus and E. transsylvanicus, which accumulated Cd according to the high bioaccwnulation factor (BAF) values. We conclude that the centipedes C. flavidus, C. anomalans and E. transsylvanicus can be used as suitable bioindicators of trace element exposure. Their ability to accumulate trace elements was different and depends on their physiology and lifestyle as well as the route of exposure.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Metal and Metalloid bioaccumulation in three centipedes (Chilopoda)",
number = "3",
volume = "74",
doi = "10.2298/ABS220514019M",
pages = "207-215"
}

Mitić, B., Borković Mitić, S., Stojsavljević, A., Sojanović, D., Pavlović, S., Vasiljević, L.,& Ristić, N.. (2022). Metal and Metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 74(3), 207-215.
https://doi.org/10.2298/ABS220514019M

Mitić B, Borković Mitić S, Stojsavljević A, Sojanović D, Pavlović S, Vasiljević L, Ristić N. Metal and Metalloid bioaccumulation in three centipedes (Chilopoda). in Archives of Biological Sciences. 2022;74(3):207-215.
doi:10.2298/ABS220514019M .

Mitić, Bojan, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Sojanović, Dalibor, Pavlović, Slađan, Vasiljević, Ljubica, Ristić, Nataša, "Metal and Metalloid bioaccumulation in three centipedes (Chilopoda)" in Archives of Biological Sciences, 74, no. 3 (2022):207-215,
https://doi.org/10.2298/ABS220514019M . .

TY  - JOUR
AU  - Jagodić, Jovana
AU  - Rovčanin, Branislav
AU  - Borković Mitić, Slavica
AU  - Vujotić, Ljiljana
AU  - Avdin, Viacheslav
AU  - Manojlović, Dragan
AU  - Stojsavljević, Aleksandar
PY  - 2021
UR  - https://doi.org/10.1007/s11356-021-14013-2
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4236
AB  - Studies indicate that the soil, water and consequently foodstuffs in Serbia are significantly poor in zinc (Zn), and thus, it is likely that there is a Zn deficiency in the Serbian population. This study examined the Zn status in multiple clinical samples, including body fluids (serum, cerebrospinal fluid), whole blood and Zn-rich solid tissues (thyroid and brain tissue). Differences between sex and age were also considered, and comparative analysis of Zn status with other world populations was performed. Serum samples from a large number of Serbian adults approximately had twofold lower Zn amounts when compared to other populations. A similar trend was obtained for whole blood. Males had significantly higher amounts of Zn in serum, whole blood and thyroid tissue samples than females. Higher amounts of Zn were observed in the group older than 50 years. Importantly, in thyroid and brain tissues, Zn was 10- and 20-fold lower, respectively, than reported in the literature. Our results indicate that the population in Serbia could be considered Zn deficient. Therefore, adequate oral Zn supplementation and/or foodstuff fortification should be considered to prevent the deleterious effects caused by Zn deficiency.
PB  - Springer Science and Business Media Deutschland GmbH
T2  - Environmental Science and Pollution Research
T1  - Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues
IS  - 34
VL  - 28
DO  - 10.1007/s11356-021-14013-2
SP  - 47439
EP  - 47446
ER  - 

@article{
author = "Jagodić, Jovana and Rovčanin, Branislav and Borković Mitić, Slavica and Vujotić, Ljiljana and Avdin, Viacheslav and Manojlović, Dragan and Stojsavljević, Aleksandar",
year = "2021",
abstract = "Studies indicate that the soil, water and consequently foodstuffs in Serbia are significantly poor in zinc (Zn), and thus, it is likely that there is a Zn deficiency in the Serbian population. This study examined the Zn status in multiple clinical samples, including body fluids (serum, cerebrospinal fluid), whole blood and Zn-rich solid tissues (thyroid and brain tissue). Differences between sex and age were also considered, and comparative analysis of Zn status with other world populations was performed. Serum samples from a large number of Serbian adults approximately had twofold lower Zn amounts when compared to other populations. A similar trend was obtained for whole blood. Males had significantly higher amounts of Zn in serum, whole blood and thyroid tissue samples than females. Higher amounts of Zn were observed in the group older than 50 years. Importantly, in thyroid and brain tissues, Zn was 10- and 20-fold lower, respectively, than reported in the literature. Our results indicate that the population in Serbia could be considered Zn deficient. Therefore, adequate oral Zn supplementation and/or foodstuff fortification should be considered to prevent the deleterious effects caused by Zn deficiency.",
publisher = "Springer Science and Business Media Deutschland GmbH",
journal = "Environmental Science and Pollution Research",
title = "Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues",
number = "34",
volume = "28",
doi = "10.1007/s11356-021-14013-2",
pages = "47439-47446"
}

Jagodić, J., Rovčanin, B., Borković Mitić, S., Vujotić, L., Avdin, V., Manojlović, D.,& Stojsavljević, A.. (2021). Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues. in Environmental Science and Pollution Research
Springer Science and Business Media Deutschland GmbH., 28(34), 47439-47446.
https://doi.org/10.1007/s11356-021-14013-2

Jagodić J, Rovčanin B, Borković Mitić S, Vujotić L, Avdin V, Manojlović D, Stojsavljević A. Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues. in Environmental Science and Pollution Research. 2021;28(34):47439-47446.
doi:10.1007/s11356-021-14013-2 .

Jagodić, Jovana, Rovčanin, Branislav, Borković Mitić, Slavica, Vujotić, Ljiljana, Avdin, Viacheslav, Manojlović, Dragan, Stojsavljević, Aleksandar, "Possible zinc deficiency in the Serbian population: examination of body fluids, whole blood and solid tissues" in Environmental Science and Pollution Research, 28, no. 34 (2021):47439-47446,
https://doi.org/10.1007/s11356-021-14013-2 . .

TY  - JOUR
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Mitić, Bojan
AU  - Manojlović, Dragan
AU  - Pavlović, Slađan
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4344
AB  - There are only a few reports examining the impact of oxidative stress in patients with benign and
malignant brain tumors. In this study we investigated whether there are changes in antioxidant
system (AOS) parameters and key trace elements between control, benign and malignant brain
tissues. The study also aimed to examine correlations between the analyzed parameters. The study
enrolled both types of brain tumors, benign tumors (BT) and malignant tumors (MT). The results
were compared with control tissue (CT) without tumor infiltration collected from patients with BT.
The following antioxidant parameters were determined: activities of total, manganese‑containing,
and copper/zinc‑containing superoxide dismutase (TotSOD, MnSOD and CuZnSOD), activities of
catalase, glutathione peroxidase, glutathione S‑transferase, glutathione reductase and acetylcholine
esterase (AChE), the concentrations of glutathione and sulfhydryl groups and of manganese (Mn),
copper (Cu), zinc (Zn), and selenium (Se). BT and MT had altered activities/levels of multiple AOS
parameters as compared to CT, indicating that tumor cells had an altered cell metabolism and changes
in AOS represent adaptive response to increased oxidative stress. Low MnSOD and AChE and high
GST activities were significant for distinguishing between MT and CT. Malignant tissue was also
characterized by lower Mn and Cu concentrations relative to CT and BT. Principal Component Analysis
clearly discriminated BT from CT and MT (PC1, 66.97%), while PC2 clearly discriminated CT from BT
and MT (33.03%). Most correlative relationships were associated with Se in the BT group and Cu in the
MT group. The results of this study reveal differences between the AOS parameters and the essential
trace elements between the analyzed groups. The observed dysregulations show that oxidative stress
could have an important role in disrupting brain homeostasis and its presence in the pathogenesis of
benign and malignant brain tumors.
PB  - Springer Nature
T2  - Scientifc Reports
T1  - Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues
VL  - 11
DO  - 10.1038/s41598-021-94302-5
SP  - 14776
ER  - 

@article{
author = "Borković Mitić, Slavica and Stojsavljević, Aleksandar and Vujotić, Ljiljana and Matić, Siniša and Mitić, Bojan and Manojlović, Dragan and Pavlović, Slađan",
year = "2021",
abstract = "There are only a few reports examining the impact of oxidative stress in patients with benign and
malignant brain tumors. In this study we investigated whether there are changes in antioxidant
system (AOS) parameters and key trace elements between control, benign and malignant brain
tissues. The study also aimed to examine correlations between the analyzed parameters. The study
enrolled both types of brain tumors, benign tumors (BT) and malignant tumors (MT). The results
were compared with control tissue (CT) without tumor infiltration collected from patients with BT.
The following antioxidant parameters were determined: activities of total, manganese‑containing,
and copper/zinc‑containing superoxide dismutase (TotSOD, MnSOD and CuZnSOD), activities of
catalase, glutathione peroxidase, glutathione S‑transferase, glutathione reductase and acetylcholine
esterase (AChE), the concentrations of glutathione and sulfhydryl groups and of manganese (Mn),
copper (Cu), zinc (Zn), and selenium (Se). BT and MT had altered activities/levels of multiple AOS
parameters as compared to CT, indicating that tumor cells had an altered cell metabolism and changes
in AOS represent adaptive response to increased oxidative stress. Low MnSOD and AChE and high
GST activities were significant for distinguishing between MT and CT. Malignant tissue was also
characterized by lower Mn and Cu concentrations relative to CT and BT. Principal Component Analysis
clearly discriminated BT from CT and MT (PC1, 66.97%), while PC2 clearly discriminated CT from BT
and MT (33.03%). Most correlative relationships were associated with Se in the BT group and Cu in the
MT group. The results of this study reveal differences between the AOS parameters and the essential
trace elements between the analyzed groups. The observed dysregulations show that oxidative stress
could have an important role in disrupting brain homeostasis and its presence in the pathogenesis of
benign and malignant brain tumors.",
publisher = "Springer Nature",
journal = "Scientifc Reports",
title = "Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues",
volume = "11",
doi = "10.1038/s41598-021-94302-5",
pages = "14776"
}

Borković Mitić, S., Stojsavljević, A., Vujotić, L., Matić, S., Mitić, B., Manojlović, D.,& Pavlović, S.. (2021). Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues. in Scientifc Reports
Springer Nature., 11, 14776.
https://doi.org/10.1038/s41598-021-94302-5

Borković Mitić S, Stojsavljević A, Vujotić L, Matić S, Mitić B, Manojlović D, Pavlović S. Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues. in Scientifc Reports. 2021;11:14776.
doi:10.1038/s41598-021-94302-5 .

Borković Mitić, Slavica, Stojsavljević, Aleksandar, Vujotić, Ljiljana, Matić, Siniša, Mitić, Bojan, Manojlović, Dragan, Pavlović, Slađan, "Differences between antioxidant defense parameters and specific trace element concentrations in healthy, benign, and malignant brain tissues" in Scientifc Reports, 11 (2021):14776,
https://doi.org/10.1038/s41598-021-94302-5 . .

TY  - JOUR
AU  - Pavlović, Slađan
AU  - Krizmanić, Imre
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mitić, Bojan
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4054
AB  - We investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione
S-transferase (GST) in the whole body of fire salamander larvae (Salamandra salamandra) from two
localities on Mt. Avala (AVS and ABP) and one locality on Mt. Vršački Breg (VSB), Serbia. We also
determined the total glutathione (GSH) and sulfhydryl group (SH) contents, as well as the concentrations of
manganese (Mn), copper (Cu), zinc (Zn), selenium (Se), arsenic (As), cadmium (Cd), lead (Pb) and uranium
(U). The obtained results show that animals from VSB had significantly lower weights and lengths than
animals from AVS and ABP. The activities of all investigated enzymes were significantly higher, while the
SH content was significantly lower in animals from VSB compared to those from AVS and ABP. No
correlations between trace-element concentrations in water and animal tissue were observed. We concluded
that the obtained results were more likely a consequence of the combination of developmental differences
and the effects of different habitat conditions, environmental and anthropogenic influences than of
concentrations of trace elements in the water alone.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)
IS  - 4
VL  - 72
DO  - 10.2298/ABS200825043P
SP  - 491
EP  - 501
ER  - 

@article{
author = "Pavlović, Slađan and Krizmanić, Imre and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mitić, Bojan",
year = "2020",
abstract = "We investigated the activities of superoxide dismutase (SOD), catalase (CAT), glutathione
peroxidase (GSH-Px), glutathione reductase (GR) and the phase II biotransformation enzyme glutathione
S-transferase (GST) in the whole body of fire salamander larvae (Salamandra salamandra) from two
localities on Mt. Avala (AVS and ABP) and one locality on Mt. Vršački Breg (VSB), Serbia. We also
determined the total glutathione (GSH) and sulfhydryl group (SH) contents, as well as the concentrations of
manganese (Mn), copper (Cu), zinc (Zn), selenium (Se), arsenic (As), cadmium (Cd), lead (Pb) and uranium
(U). The obtained results show that animals from VSB had significantly lower weights and lengths than
animals from AVS and ABP. The activities of all investigated enzymes were significantly higher, while the
SH content was significantly lower in animals from VSB compared to those from AVS and ABP. No
correlations between trace-element concentrations in water and animal tissue were observed. We concluded
that the obtained results were more likely a consequence of the combination of developmental differences
and the effects of different habitat conditions, environmental and anthropogenic influences than of
concentrations of trace elements in the water alone.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)",
number = "4",
volume = "72",
doi = "10.2298/ABS200825043P",
pages = "491-501"
}

Pavlović, S., Krizmanić, I., Borković Mitić, S., Stojsavljević, A.,& Mitić, B.. (2020). A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia). in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 72(4), 491-501.
https://doi.org/10.2298/ABS200825043P

Pavlović S, Krizmanić I, Borković Mitić S, Stojsavljević A, Mitić B. A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia). in Archives of Biological Sciences. 2020;72(4):491-501.
doi:10.2298/ABS200825043P .

Pavlović, Slađan, Krizmanić, Imre, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mitić, Bojan, "A first record of the antioxidant defense and selected trace elements in Salamandra salamandra larvae on Mt. Avala and Mt. Vršački Breg (Serbia)" in Archives of Biological Sciences, 72, no. 4 (2020):491-501,
https://doi.org/10.2298/ABS200825043P . .

TY  - JOUR
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mutić, Jelena
AU  - Baščarević, Vladimir
AU  - Joković, Miloš
AU  - Pavlović, Slađan
PY  - 2020
UR  - https://www.termedia.pl/Association-between-oxidative-stress-biomarkers-and-concentrations-of-some-metal-ions-in-the-blood-of-patients-with-brain-tumors-and-hydrocephalus,19,37458,1,1.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3459
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3713
AB  - IntroductionAny substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.Material and methodsOur study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.ResultsThe higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.ConclusionsThere are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.
PB  - Termedia & Banach
T2  - Archives of Medical Science
T1  - Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus
IS  - 4
VL  - 16
DO  - 10.5114/aoms.2019.87409
SP  - 811
EP  - 819
ER  - 

@article{
author = "Vujotić, Ljiljana and Matić, Siniša and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mutić, Jelena and Baščarević, Vladimir and Joković, Miloš and Pavlović, Slađan",
year = "2020",
abstract = "IntroductionAny substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.Material and methodsOur study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.ResultsThe higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.ConclusionsThere are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.",
publisher = "Termedia & Banach",
journal = "Archives of Medical Science",
title = "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus",
number = "4",
volume = "16",
doi = "10.5114/aoms.2019.87409",
pages = "811-819"
}

Vujotić, L., Matić, S., Borković Mitić, S., Stojsavljević, A., Mutić, J., Baščarević, V., Joković, M.,& Pavlović, S.. (2020). Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science
Termedia & Banach., 16(4), 811-819.
https://doi.org/10.5114/aoms.2019.87409

Vujotić L, Matić S, Borković Mitić S, Stojsavljević A, Mutić J, Baščarević V, Joković M, Pavlović S. Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science. 2020;16(4):811-819.
doi:10.5114/aoms.2019.87409 .

Vujotić, Ljiljana, Matić, Siniša, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mutić, Jelena, Baščarević, Vladimir, Joković, Miloš, Pavlović, Slađan, "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus" in Archives of Medical Science, 16, no. 4 (2020):811-819,
https://doi.org/10.5114/aoms.2019.87409 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Jagodić, Jovana
AU  - Vujotić, Ljiljana
AU  - Borković Mitić, Slavica
AU  - Rašić-Milutinović, Zorica
AU  - Jovanović, Dragana
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2020
UR  - http://link.springer.com/10.1007/s11356-019-06936-8
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3530
AB  - This study was aimed to determine reference values (RVs) for the manganese (Mn), copper (Cu), zinc (Zn), and selenium (Se) in the whole blood (B) and serum (S) samples of the Serbian population. Blood specimens were collected from healthy persons (n = 295; women/men ratio = 149/146; mean age: 42 ± 2 years). The RVs were calculated as lower limit (LL) and upper limit (UL) of the 95% confidence interval (CI) and were expressed as percentiles (P) in the range from P2.5 to P97.5. The influences of sex, age, and smoking habits on element profiles were considered. It was found that the contents of B-Cu and S-Cu were higher in women, while the contents of B-Zn and S-Zn were higher in men. Both trace elements were significantly increased in a group of persons above 40 when compared to a younger persons (≤ 40 years). According to smoking habits, increased content was found only for S-Mn in the nonsmoker's group (p < 0.05). Comparing our results to the results reported in other population groups worldwide, the Serbian population had significantly reduced content of Se in both types of samples. This finding could highlight the deficiency of Se in the investigated Serbian population and could contribute to the better understanding of the molecular basis for the increased incidence of thyroid and other diseases in which selenium plays a key role.
T2  - Environmental Science and Pollution Research
T1  - Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency.
IS  - 2
VL  - 27
DO  - 10.1007/s11356-019-06936-8
SP  - 1397
EP  - 1405
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Jagodić, Jovana and Vujotić, Ljiljana and Borković Mitić, Slavica and Rašić-Milutinović, Zorica and Jovanović, Dragana and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2020",
abstract = "This study was aimed to determine reference values (RVs) for the manganese (Mn), copper (Cu), zinc (Zn), and selenium (Se) in the whole blood (B) and serum (S) samples of the Serbian population. Blood specimens were collected from healthy persons (n = 295; women/men ratio = 149/146; mean age: 42 ± 2 years). The RVs were calculated as lower limit (LL) and upper limit (UL) of the 95% confidence interval (CI) and were expressed as percentiles (P) in the range from P2.5 to P97.5. The influences of sex, age, and smoking habits on element profiles were considered. It was found that the contents of B-Cu and S-Cu were higher in women, while the contents of B-Zn and S-Zn were higher in men. Both trace elements were significantly increased in a group of persons above 40 when compared to a younger persons (≤ 40 years). According to smoking habits, increased content was found only for S-Mn in the nonsmoker's group (p < 0.05). Comparing our results to the results reported in other population groups worldwide, the Serbian population had significantly reduced content of Se in both types of samples. This finding could highlight the deficiency of Se in the investigated Serbian population and could contribute to the better understanding of the molecular basis for the increased incidence of thyroid and other diseases in which selenium plays a key role.",
journal = "Environmental Science and Pollution Research",
title = "Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency.",
number = "2",
volume = "27",
doi = "10.1007/s11356-019-06936-8",
pages = "1397-1405"
}

Stojsavljević, A., Jagodić, J., Vujotić, L., Borković Mitić, S., Rašić-Milutinović, Z., Jovanović, D., Gavrović-Jankulović, M.,& Manojlović, D.. (2020). Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency.. in Environmental Science and Pollution Research, 27(2), 1397-1405.
https://doi.org/10.1007/s11356-019-06936-8

Stojsavljević A, Jagodić J, Vujotić L, Borković Mitić S, Rašić-Milutinović Z, Jovanović D, Gavrović-Jankulović M, Manojlović D. Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency.. in Environmental Science and Pollution Research. 2020;27(2):1397-1405.
doi:10.1007/s11356-019-06936-8 .

Stojsavljević, Aleksandar, Jagodić, Jovana, Vujotić, Ljiljana, Borković Mitić, Slavica, Rašić-Milutinović, Zorica, Jovanović, Dragana, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Reference values for trace essential elements in the whole blood and serum samples of the adult Serbian population: significance of selenium deficiency." in Environmental Science and Pollution Research, 27, no. 2 (2020):1397-1405,
https://doi.org/10.1007/s11356-019-06936-8 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Vujotić, Ljiljana
AU  - Rovčanin, Branislav
AU  - Borković Mitić, Slavica
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3624
AB  - The pathogenesis of malignant brain tumors (MBTs) should be better understood due to the evident association between prolonged exposure to metals and increased risk of MBTs. The present research aimed to find trace metals that could contribute to the pathogenesis of MBTs. Essential trace elements (Mn, Co, Zn, Cu, Se) and relevant toxic metals (Al, Ni, As, Sr, Cd, Ce, Pt, Pb, U) in the serum, cell fraction (CF), cerebrospinal fluid (CSF) and cancerous tissue (CT) samples of MBT patients were analyzed. The results were compared with sex- and age-matched control groups. For the first time, this research showed that elemental profiles of serum, CF, CSF and CT samples in MBT patients were significantly altered compared to the appropriate controls, as well as that higher contents of trace elements (particularly Mn, Se, and Pb) could be involved in the pathogenesis of MBTs. However, the most noticeable change found was the elevated U content, indicating its considerable role as a major cerebral discriminator of the presence/absence of MBTs. The U/Se ratio could be considered as an appropriate blood marker in diagnostic MBT evaluation. The reported results could contribute to better understanding of the poorly understood pathogenesis of MBTs. Furthermore, the reported results could highlight a molecular basis for the pathophysiological changes caused by the hazardous effects of trace metals on brain homeostasis.
T2  - Scientific Reports
T1  - Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60774-0
SP  - 3816
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Vujotić, Ljiljana and Rovčanin, Branislav and Borković Mitić, Slavica and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2020",
abstract = "The pathogenesis of malignant brain tumors (MBTs) should be better understood due to the evident association between prolonged exposure to metals and increased risk of MBTs. The present research aimed to find trace metals that could contribute to the pathogenesis of MBTs. Essential trace elements (Mn, Co, Zn, Cu, Se) and relevant toxic metals (Al, Ni, As, Sr, Cd, Ce, Pt, Pb, U) in the serum, cell fraction (CF), cerebrospinal fluid (CSF) and cancerous tissue (CT) samples of MBT patients were analyzed. The results were compared with sex- and age-matched control groups. For the first time, this research showed that elemental profiles of serum, CF, CSF and CT samples in MBT patients were significantly altered compared to the appropriate controls, as well as that higher contents of trace elements (particularly Mn, Se, and Pb) could be involved in the pathogenesis of MBTs. However, the most noticeable change found was the elevated U content, indicating its considerable role as a major cerebral discriminator of the presence/absence of MBTs. The U/Se ratio could be considered as an appropriate blood marker in diagnostic MBT evaluation. The reported results could contribute to better understanding of the poorly understood pathogenesis of MBTs. Furthermore, the reported results could highlight a molecular basis for the pathophysiological changes caused by the hazardous effects of trace metals on brain homeostasis.",
journal = "Scientific Reports",
title = "Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60774-0",
pages = "3816"
}

Stojsavljević, A., Vujotić, L., Rovčanin, B., Borković Mitić, S., Gavrović-Jankulović, M.,& Manojlović, D.. (2020). Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors. in Scientific Reports, 10(1), 3816.
https://doi.org/10.1038/s41598-020-60774-0

Stojsavljević A, Vujotić L, Rovčanin B, Borković Mitić S, Gavrović-Jankulović M, Manojlović D. Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors. in Scientific Reports. 2020;10(1):3816.
doi:10.1038/s41598-020-60774-0 .

Stojsavljević, Aleksandar, Vujotić, Ljiljana, Rovčanin, Branislav, Borković Mitić, Slavica, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Assessment of trace metal alterations in the blood, cerebrospinal fluid and tissue samples of patients with malignant brain tumors" in Scientific Reports, 10, no. 1 (2020):3816,
https://doi.org/10.1038/s41598-020-60774-0 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa
AU  - Borković Mitić, Slavica
AU  - Paunović, Ivan
AU  - Diklić, Aleksandar
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2019
UR  - http://link.springer.com/10.1007/s12403-019-00309-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3359
AB  - The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards.
T2  - Exposure and Health
T1  - Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease
DO  - 10.1007/s12403-019-00309-9
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa and Borković Mitić, Slavica and Paunović, Ivan and Diklić, Aleksandar and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2019",
abstract = "The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards.",
journal = "Exposure and Health",
title = "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease",
doi = "10.1007/s12403-019-00309-9"
}

Stojsavljević, A., Rovčanin, B., Krstić, Đ., Borković Mitić, S., Paunović, I., Diklić, A., Gavrović-Jankulović, M.,& Manojlović, D.. (2019). Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease. in Exposure and Health.
https://doi.org/10.1007/s12403-019-00309-9

Stojsavljević A, Rovčanin B, Krstić Đ, Borković Mitić S, Paunović I, Diklić A, Gavrović-Jankulović M, Manojlović D. Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease. in Exposure and Health. 2019;.
doi:10.1007/s12403-019-00309-9 .

Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa, Borković Mitić, Slavica, Paunović, Ivan, Diklić, Aleksandar, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease" in Exposure and Health (2019),
https://doi.org/10.1007/s12403-019-00309-9 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa
AU  - Jagodić, Jovana
AU  - Borković Mitić, Slavica
AU  - Paunović, Ivan
AU  - Živaljević, Vladan
AU  - Mitić, Bojan
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0946672X19300070?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3435
AB  - BACKGROUND The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio’s (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. METHODS The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). RESULTS Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. CONCLUSION It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology
VL  - 55
DO  - 10.1016/J.JTEMB.2019.06.009
SP  - 190
EP  - 195
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa and Jagodić, Jovana and Borković Mitić, Slavica and Paunović, Ivan and Živaljević, Vladan and Mitić, Bojan and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2019",
abstract = "BACKGROUND The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio’s (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. METHODS The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). RESULTS Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. CONCLUSION It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology",
volume = "55",
doi = "10.1016/J.JTEMB.2019.06.009",
pages = "190-195"
}

Stojsavljević, A., Rovčanin, B., Krstić, Đ., Jagodić, J., Borković Mitić, S., Paunović, I., Živaljević, V., Mitić, B., Gavrović-Jankulović, M.,& Manojlović, D.. (2019). Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology. in Journal of Trace Elements in Medicine and Biology, 55, 190-195.
https://doi.org/10.1016/J.JTEMB.2019.06.009

Stojsavljević A, Rovčanin B, Krstić Đ, Jagodić J, Borković Mitić S, Paunović I, Živaljević V, Mitić B, Gavrović-Jankulović M, Manojlović D. Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology. in Journal of Trace Elements in Medicine and Biology. 2019;55:190-195.
doi:10.1016/J.JTEMB.2019.06.009 .

Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa, Jagodić, Jovana, Borković Mitić, Slavica, Paunović, Ivan, Živaljević, Vladan, Mitić, Bojan, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology" in Journal of Trace Elements in Medicine and Biology, 55 (2019):190-195,
https://doi.org/10.1016/J.JTEMB.2019.06.009 . .

TY  - JOUR
AU  - Vujotić, Ljiljana
AU  - Matić, Siniša
AU  - Borković Mitić, Slavica
AU  - Stojsavljević, Aleksandar
AU  - Mutić, Jelena
AU  - Baščarević, Vladimir
AU  - Joković, Miloš
AU  - Pavlović, Slađan
PY  - 2019
UR  - https://www.termedia.pl/Association-between-oxidative-stress-biomarkers-and-concentrations-of-some-metal-ions-in-the-blood-of-patients-with-brain-tumors-and-hydrocephalus,19,37458,1,1.html
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3459
AB  - Introduction
Any substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.

Material and methods
Our study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.

Results
The higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.

Conclusions
There are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.
PB  - Termedia & Banach
T2  - Archives of Medical Science
T1  - Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus
DO  - 10.5114/aoms.2019.87409
ER  - 

@article{
author = "Vujotić, Ljiljana and Matić, Siniša and Borković Mitić, Slavica and Stojsavljević, Aleksandar and Mutić, Jelena and Baščarević, Vladimir and Joković, Miloš and Pavlović, Slađan",
year = "2019",
abstract = "Introduction
Any substance that induces production of free radicals can be a potential cause of brain damage. The aim of our study was to investigate the relationship between some metal ions and oxidative stress biomarkers in the blood of patients with brain tumor and hydrocephalus.

Material and methods
Our study included 27 control subjects, 24 patients with brain tumor and 21 patients with hydrocephalus. The activities of superoxide dismutase (CuZn SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), glutathione S-transferase (GST) and acetyl cholinesterase (AChE), as well as concentrations of reduced glutathione (GSH), lipid peroxides (TBARS) and sulfhydryl groups (SH) were analyzed in the plasma and red blood cells (RBCs) of patients. We also determined the concentrations of Mn, Ni, Co, Cu, Zn, As, Se, Cd, Hg and Fe.

Results
The higher activity of SOD and concentration of GSH in both investigated groups could indicate higher oxidative stress. We also observed decreased levels of SH groups in both groups of patients. In both groups of patients we detected decreased concentrations of Ni, Co, Zn and Fe (and Mn in brain tumor patients) and increased concentrations of As, Se and Cd in the blood. Interestingly, we observed a higher concentration of Cd in both plasma and RBCs of hydrocephalus patients compared to the patients with brain tumor.

Conclusions
There are strong correlations between some metal ion concentrations and certain oxidative stress biomarkers in the blood of patients, which supports our hypothesis, but the observed trend needs to be further investigated.",
publisher = "Termedia & Banach",
journal = "Archives of Medical Science",
title = "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus",
doi = "10.5114/aoms.2019.87409"
}

Vujotić, L., Matić, S., Borković Mitić, S., Stojsavljević, A., Mutić, J., Baščarević, V., Joković, M.,& Pavlović, S.. (2019). Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science
Termedia & Banach..
https://doi.org/10.5114/aoms.2019.87409

Vujotić L, Matić S, Borković Mitić S, Stojsavljević A, Mutić J, Baščarević V, Joković M, Pavlović S. Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus. in Archives of Medical Science. 2019;.
doi:10.5114/aoms.2019.87409 .

Vujotić, Ljiljana, Matić, Siniša, Borković Mitić, Slavica, Stojsavljević, Aleksandar, Mutić, Jelena, Baščarević, Vladimir, Joković, Miloš, Pavlović, Slađan, "Association between oxidative stress biomarkers and concentrations of some metal ions in the blood of patients with brain tumors and hydrocephalus" in Archives of Medical Science (2019),
https://doi.org/10.5114/aoms.2019.87409 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa
AU  - Borković Mitić, Slavica
AU  - Paunović, Ivan
AU  - Kodranov, Igor
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0147651319308103?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3450
AB  - Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.
T2  - Ecotoxicology and Environmental Safety
T1  - Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.
VL  - 183
DO  - 10.1016/j.ecoenv.2019.109479
SP  - 109479
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa and Borković Mitić, Slavica and Paunović, Ivan and Kodranov, Igor and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2019",
abstract = "Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.",
journal = "Ecotoxicology and Environmental Safety",
title = "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.",
volume = "183",
doi = "10.1016/j.ecoenv.2019.109479",
pages = "109479"
}

Stojsavljević, A., Rovčanin, B., Krstić, Đ., Borković Mitić, S., Paunović, I., Kodranov, I., Gavrović-Jankulović, M.,& Manojlović, D.. (2019). Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.. in Ecotoxicology and Environmental Safety, 183, 109479.
https://doi.org/10.1016/j.ecoenv.2019.109479

Stojsavljević A, Rovčanin B, Krstić Đ, Borković Mitić S, Paunović I, Kodranov I, Gavrović-Jankulović M, Manojlović D. Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.. in Ecotoxicology and Environmental Safety. 2019;183:109479.
doi:10.1016/j.ecoenv.2019.109479 .

Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa, Borković Mitić, Slavica, Paunović, Ivan, Kodranov, Igor, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases." in Ecotoxicology and Environmental Safety, 183 (2019):109479,
https://doi.org/10.1016/j.ecoenv.2019.109479 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Škrivanj, Sandra
AU  - Trifković, Jelena
AU  - Đoković, Nenad
AU  - Trifunović, Srećko R
AU  - Borković Mitić, Slavica
AU  - Manojlović, Dragan
PY  - 2019
UR  - http://link.springer.com/10.1007/s11356-019-04796-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3336
AB  - Pollution caused by heavy metals affects all forms of life. The aim of the study was to determine the content of toxic (Sr, Ni, Pb, V, Cd, U, Rb, As) and essential (Na, K, Ca, Mg, Zn, Cu, Se, Mn, Cr, Mo, Co) metals in the bone and whole blood samples, in regard to clinical means of long- and short-term exposure, respectively. For this purpose, the cortical and trabecular parts of femoral neck, as well as the blood samples, were collected to quantify bone-important metals by inductively coupled plasma (ICP)-based techniques. According to principal component analysis (PCA), the most influential metal discriminating blood samples was Cu, while all other quantified elements were present in higher amounts in the bones. Additionally, trabecular bones (TBs) could be characterized by higher content of Mo, Cr, V, Mn, Co, As, and Ni compared to cortical bones (CBs). Linear discrimination analysis (LDA) was successfully applied to distinguish trabecular bone from the cortical bone. Significant correlation between essential Ca and toxic Sr with other elements was found and discussed. This study provides novel data on the effects of metal pollutants on bone health hazards. The results obtained for investigating metals may serve as a baseline for further clinical investigations in the orthopedic fields.
T2  - Environmental Science and Pollution Research
T1  - The content of toxic and essential elements in trabecular and cortical femoral neck: a correlation with whole blood samples.
DO  - 10.1007/s11356-019-04796-w
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Škrivanj, Sandra and Trifković, Jelena and Đoković, Nenad and Trifunović, Srećko R and Borković Mitić, Slavica and Manojlović, Dragan",
year = "2019",
abstract = "Pollution caused by heavy metals affects all forms of life. The aim of the study was to determine the content of toxic (Sr, Ni, Pb, V, Cd, U, Rb, As) and essential (Na, K, Ca, Mg, Zn, Cu, Se, Mn, Cr, Mo, Co) metals in the bone and whole blood samples, in regard to clinical means of long- and short-term exposure, respectively. For this purpose, the cortical and trabecular parts of femoral neck, as well as the blood samples, were collected to quantify bone-important metals by inductively coupled plasma (ICP)-based techniques. According to principal component analysis (PCA), the most influential metal discriminating blood samples was Cu, while all other quantified elements were present in higher amounts in the bones. Additionally, trabecular bones (TBs) could be characterized by higher content of Mo, Cr, V, Mn, Co, As, and Ni compared to cortical bones (CBs). Linear discrimination analysis (LDA) was successfully applied to distinguish trabecular bone from the cortical bone. Significant correlation between essential Ca and toxic Sr with other elements was found and discussed. This study provides novel data on the effects of metal pollutants on bone health hazards. The results obtained for investigating metals may serve as a baseline for further clinical investigations in the orthopedic fields.",
journal = "Environmental Science and Pollution Research",
title = "The content of toxic and essential elements in trabecular and cortical femoral neck: a correlation with whole blood samples.",
doi = "10.1007/s11356-019-04796-w"
}

Stojsavljević, A., Škrivanj, S., Trifković, J., Đoković, N., Trifunović, S. R., Borković Mitić, S.,& Manojlović, D.. (2019). The content of toxic and essential elements in trabecular and cortical femoral neck: a correlation with whole blood samples.. in Environmental Science and Pollution Research.
https://doi.org/10.1007/s11356-019-04796-w

Stojsavljević A, Škrivanj S, Trifković J, Đoković N, Trifunović SR, Borković Mitić S, Manojlović D. The content of toxic and essential elements in trabecular and cortical femoral neck: a correlation with whole blood samples.. in Environmental Science and Pollution Research. 2019;.
doi:10.1007/s11356-019-04796-w .

Stojsavljević, Aleksandar, Škrivanj, Sandra, Trifković, Jelena, Đoković, Nenad, Trifunović, Srećko R, Borković Mitić, Slavica, Manojlović, Dragan, "The content of toxic and essential elements in trabecular and cortical femoral neck: a correlation with whole blood samples." in Environmental Science and Pollution Research (2019),
https://doi.org/10.1007/s11356-019-04796-w . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Borković Mitić, Slavica
AU  - Vujotić, Ljiljana
AU  - Grujičić, Danica
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0147651318311783?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3192
AB  - The purpose of this study was to establish reference values (RVs) for the occupationally- and environmentally-important toxic elements in the whole blood of adult Serbian population for the first time. Contaminated drinking water with arsenic, high share of smokers in the country, removing tetraethyl lead from the gasoline and war attack at the end of the twentieth century were some of the reasons to provide background information for arsenic (As), cadmium (Cd), lead (Pb), thorium (Th), and uranium (U) in the blood of the Serbian population. The whole blood samples were collected from the healthy respondents living in the Belgrade and surrounding areas of the capital (n = 305; w/m ratio = 154/151; mean age: 41 ± 2). The concentrations of toxic metals were determined by inductively coupled plasma-mass spectrometry (ICP-MS). Reference values were estimated as the lower limit (LL) and upper limit (UL) of the 95% confidence interval (CI), together with the selected percentiles (P2.5-P97.5). The obtained geometric mean (GM) for As, Cd, Pb, Th, and U were: 0.50 ng/g, 0.32 ng/g, 20.94 ng/g, 0.30 ng/g, and 0.06 ng/g, respectively. The influences of age, sex and lifestyle on results were considered. Women have significantly higher levels of Cd and Th than men. The increased level of Th was observed in the aged group below 40 years, while smokers had significantly higher levels of Pb and double higher level of Cd in the blood than non-smokers (p < 0.05). In comparison with other population groups worldwide, the Serbian population had significantly higher levels of Th and U (up to 100 times higher). These findings could contribute to better understanding of the molecular basis for the development of various health hazards, including the increased incidence of cancer among the Serbian population which need be confirmed by clinical studies.
T2  - Ecotoxicology and Environmental Safety
T1  - The human biomonitoring study in Serbia: Background levels for arsenic, cadmium, lead, thorium and uranium in the whole blood of adult Serbian population.
VL  - 169
DO  - 10.1016/j.ecoenv.2018.11.043
SP  - 402
EP  - 409
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Borković Mitić, Slavica and Vujotić, Ljiljana and Grujičić, Danica and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2018",
abstract = "The purpose of this study was to establish reference values (RVs) for the occupationally- and environmentally-important toxic elements in the whole blood of adult Serbian population for the first time. Contaminated drinking water with arsenic, high share of smokers in the country, removing tetraethyl lead from the gasoline and war attack at the end of the twentieth century were some of the reasons to provide background information for arsenic (As), cadmium (Cd), lead (Pb), thorium (Th), and uranium (U) in the blood of the Serbian population. The whole blood samples were collected from the healthy respondents living in the Belgrade and surrounding areas of the capital (n = 305; w/m ratio = 154/151; mean age: 41 ± 2). The concentrations of toxic metals were determined by inductively coupled plasma-mass spectrometry (ICP-MS). Reference values were estimated as the lower limit (LL) and upper limit (UL) of the 95% confidence interval (CI), together with the selected percentiles (P2.5-P97.5). The obtained geometric mean (GM) for As, Cd, Pb, Th, and U were: 0.50 ng/g, 0.32 ng/g, 20.94 ng/g, 0.30 ng/g, and 0.06 ng/g, respectively. The influences of age, sex and lifestyle on results were considered. Women have significantly higher levels of Cd and Th than men. The increased level of Th was observed in the aged group below 40 years, while smokers had significantly higher levels of Pb and double higher level of Cd in the blood than non-smokers (p < 0.05). In comparison with other population groups worldwide, the Serbian population had significantly higher levels of Th and U (up to 100 times higher). These findings could contribute to better understanding of the molecular basis for the development of various health hazards, including the increased incidence of cancer among the Serbian population which need be confirmed by clinical studies.",
journal = "Ecotoxicology and Environmental Safety",
title = "The human biomonitoring study in Serbia: Background levels for arsenic, cadmium, lead, thorium and uranium in the whole blood of adult Serbian population.",
volume = "169",
doi = "10.1016/j.ecoenv.2018.11.043",
pages = "402-409"
}

Stojsavljević, A., Borković Mitić, S., Vujotić, L., Grujičić, D., Gavrović-Jankulović, M.,& Manojlović, D.. (2018). The human biomonitoring study in Serbia: Background levels for arsenic, cadmium, lead, thorium and uranium in the whole blood of adult Serbian population.. in Ecotoxicology and Environmental Safety, 169, 402-409.
https://doi.org/10.1016/j.ecoenv.2018.11.043

Stojsavljević A, Borković Mitić S, Vujotić L, Grujičić D, Gavrović-Jankulović M, Manojlović D. The human biomonitoring study in Serbia: Background levels for arsenic, cadmium, lead, thorium and uranium in the whole blood of adult Serbian population.. in Ecotoxicology and Environmental Safety. 2018;169:402-409.
doi:10.1016/j.ecoenv.2018.11.043 .

Stojsavljević, Aleksandar, Borković Mitić, Slavica, Vujotić, Ljiljana, Grujičić, Danica, Gavrović-Jankulović, Marija, Manojlović, Dragan, "The human biomonitoring study in Serbia: Background levels for arsenic, cadmium, lead, thorium and uranium in the whole blood of adult Serbian population." in Ecotoxicology and Environmental Safety, 169 (2018):402-409,
https://doi.org/10.1016/j.ecoenv.2018.11.043 . .