TY  - JOUR
AU  - Trifunović, Svetlana
AU  - Šošić-Jurjević, Branka 
AU  - Ristić, Nataša
AU  - Nestorović, Nataša
AU  - Filipović, Branko
AU  - Stevanović, Ivana
AU  - Begović-Kuprešanin, Vesna
AU  - Manojlović-Stojanoski, Milica
PY  - 2023
UR  - https://www.mdpi.com/1422-0067/24/1/540
UR  - http://www.ncbi.nlm.nih.gov/pubmed/36613982
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9820254
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5402
AB  - As the mediator between the mother and fetus, the placenta allows the most appropriate environment and optimal fetal growth. The placenta of one sex sometimes has a greater ability over the other to respond to and protect against possible maternal insults. Here, we characterized sex differences in the placenta's morphological features and antioxidant status following dexamethasone (Dx) exposure. Pregnant rats were exposed to Dx or saline. The placenta was histologically and stereologically analyzed. The activity of the antioxidant enzymes, lipid peroxides (TBARS), superoxide anion and nitric oxide (NO) was measured. The decrease in placental zone volumes was more pronounced (p < 0.05) in female placentas. The volume density of PCNA-immunopositive nuclei was reduced (p < 0.05) in both sexes. The reduced (p < 0.05) antioxidant enzyme activities, enhanced TBARS and NO concentration indicate that Dx exposure triggered oxidative stress in the placenta of both fetal sexes, albeit stronger in the placenta of female fetuses. In conclusion, maternal Dx treatment reduced the size and volume of placental zones, altered placental histomorphology, decreased cell proliferation and triggered oxidative stress; however, the placentas of female fetuses exerted more significant responses to the treatment effects. The reduced placental size most probably reduced the transport of nutrients and oxygen, thus resulting in the reduced weight of fetuses, similar in both sexes. The lesser ability of the male placenta to detect and react to maternal exposure to environmental challenges may lead to long-standing health effects.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta
IS  - 1
VL  - 24
DO  - 10.3390/ijms24010540
SP  - 540
ER  - 

@article{
author = "Trifunović, Svetlana and Šošić-Jurjević, Branka  and Ristić, Nataša and Nestorović, Nataša and Filipović, Branko and Stevanović, Ivana and Begović-Kuprešanin, Vesna and Manojlović-Stojanoski, Milica",
year = "2023",
abstract = "As the mediator between the mother and fetus, the placenta allows the most appropriate environment and optimal fetal growth. The placenta of one sex sometimes has a greater ability over the other to respond to and protect against possible maternal insults. Here, we characterized sex differences in the placenta's morphological features and antioxidant status following dexamethasone (Dx) exposure. Pregnant rats were exposed to Dx or saline. The placenta was histologically and stereologically analyzed. The activity of the antioxidant enzymes, lipid peroxides (TBARS), superoxide anion and nitric oxide (NO) was measured. The decrease in placental zone volumes was more pronounced (p < 0.05) in female placentas. The volume density of PCNA-immunopositive nuclei was reduced (p < 0.05) in both sexes. The reduced (p < 0.05) antioxidant enzyme activities, enhanced TBARS and NO concentration indicate that Dx exposure triggered oxidative stress in the placenta of both fetal sexes, albeit stronger in the placenta of female fetuses. In conclusion, maternal Dx treatment reduced the size and volume of placental zones, altered placental histomorphology, decreased cell proliferation and triggered oxidative stress; however, the placentas of female fetuses exerted more significant responses to the treatment effects. The reduced placental size most probably reduced the transport of nutrients and oxygen, thus resulting in the reduced weight of fetuses, similar in both sexes. The lesser ability of the male placenta to detect and react to maternal exposure to environmental challenges may lead to long-standing health effects.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta",
number = "1",
volume = "24",
doi = "10.3390/ijms24010540",
pages = "540"
}

Trifunović, S., Šošić-Jurjević, B., Ristić, N., Nestorović, N., Filipović, B., Stevanović, I., Begović-Kuprešanin, V.,& Manojlović-Stojanoski, M.. (2023). Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta. in International Journal of Molecular Sciences
Basel: MDPI., 24(1), 540.
https://doi.org/10.3390/ijms24010540

Trifunović S, Šošić-Jurjević B, Ristić N, Nestorović N, Filipović B, Stevanović I, Begović-Kuprešanin V, Manojlović-Stojanoski M. Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta. in International Journal of Molecular Sciences. 2023;24(1):540.
doi:10.3390/ijms24010540 .

Trifunović, Svetlana, Šošić-Jurjević, Branka , Ristić, Nataša, Nestorović, Nataša, Filipović, Branko, Stevanović, Ivana, Begović-Kuprešanin, Vesna, Manojlović-Stojanoski, Milica, "Maternal Dexamethasone Exposure Induces Sex-Specific Changes in Histomorphology and Redox Homeostasis of Rat Placenta" in International Journal of Molecular Sciences, 24, no. 1 (2023):540,
https://doi.org/10.3390/ijms24010540 . .

TY  - JOUR
AU  - Dejanović, Bratislav
AU  - Begović-Kuprešanin, Vesna
AU  - Stevanović, Ivana
AU  - Lavrnja, Irena
AU  - Šošić-Jurjević, Branka 
AU  - Ninković, Milica
AU  - Trifunović, Svetlana
PY  - 2021
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46642100028D
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4651
UR  - https://www.serbiosoc.org.rs/arch/index.php/abs/article/view/6557
AB  - The use of the antidepressant drug chlorpromazine (CPZ) is linked to the occurrence of oxidative stress in some brain structures. Thus, overcoming the side effects of CPZ is of great importance. Because agmatine (AGM) can act as a free radical scavenger, it is an interesting compound as an adjunct to CPZ therapy. The aim of our study was to investigate the enzymatic parameters of oxidative stress in the hippocampus and striatum of rats after CPZ treatment, and the potential protective effects of AGM. Rats were injected as follows with (i) 1 mL/kg b.w. saline; (ii) a single intraperitoneal (i.p.) dose of CPZ (38.7 mg/kg); (iii) CPZ (38.7 mg/kg) and AGM (75 mg/kg); (iv) AGM (75 mg/kg). CPZ induced an increase in superoxide anion radical (O2 catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), were lowered in both the hippocampus striatum. Cotreatment with CPZ and AGM protected the examined brain structures by reversing the antioxidant enzyme control values. Following CPZ treatment, the effects were more pronounced for SOD and GPx in the hippocampus, the striatum. The full effect of restored superoxide production was achieved in the striatum, which points to the role of CAT. The obtained results suggest that CPZ in combination with AGM may be considered as a new treatment strategy.
PB  - Belgrade: Serbian Biological Society
T2  - Archives of Biological Sciences
T1  - Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum
IS  - 3
VL  - 73
DO  - 10.2298/abs210429028d
SP  - 353
EP  - 359
ER  - 

@article{
author = "Dejanović, Bratislav and Begović-Kuprešanin, Vesna and Stevanović, Ivana and Lavrnja, Irena and Šošić-Jurjević, Branka  and Ninković, Milica and Trifunović, Svetlana",
year = "2021",
abstract = "The use of the antidepressant drug chlorpromazine (CPZ) is linked to the occurrence of oxidative stress in some brain structures. Thus, overcoming the side effects of CPZ is of great importance. Because agmatine (AGM) can act as a free radical scavenger, it is an interesting compound as an adjunct to CPZ therapy. The aim of our study was to investigate the enzymatic parameters of oxidative stress in the hippocampus and striatum of rats after CPZ treatment, and the potential protective effects of AGM. Rats were injected as follows with (i) 1 mL/kg b.w. saline; (ii) a single intraperitoneal (i.p.) dose of CPZ (38.7 mg/kg); (iii) CPZ (38.7 mg/kg) and AGM (75 mg/kg); (iv) AGM (75 mg/kg). CPZ induced an increase in superoxide anion radical (O2 catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), were lowered in both the hippocampus striatum. Cotreatment with CPZ and AGM protected the examined brain structures by reversing the antioxidant enzyme control values. Following CPZ treatment, the effects were more pronounced for SOD and GPx in the hippocampus, the striatum. The full effect of restored superoxide production was achieved in the striatum, which points to the role of CAT. The obtained results suggest that CPZ in combination with AGM may be considered as a new treatment strategy.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Archives of Biological Sciences",
title = "Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum",
number = "3",
volume = "73",
doi = "10.2298/abs210429028d",
pages = "353-359"
}

Dejanović, B., Begović-Kuprešanin, V., Stevanović, I., Lavrnja, I., Šošić-Jurjević, B., Ninković, M.,& Trifunović, S.. (2021). Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum. in Archives of Biological Sciences
Belgrade: Serbian Biological Society., 73(3), 353-359.
https://doi.org/10.2298/abs210429028d

Dejanović B, Begović-Kuprešanin V, Stevanović I, Lavrnja I, Šošić-Jurjević B, Ninković M, Trifunović S. Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum. in Archives of Biological Sciences. 2021;73(3):353-359.
doi:10.2298/abs210429028d .

Dejanović, Bratislav, Begović-Kuprešanin, Vesna, Stevanović, Ivana, Lavrnja, Irena, Šošić-Jurjević, Branka , Ninković, Milica, Trifunović, Svetlana, "Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum" in Archives of Biological Sciences, 73, no. 3 (2021):353-359,
https://doi.org/10.2298/abs210429028d . .

TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Begović-Kuprešanin, Vesna
AU  - Peković, Sanja
AU  - Lavrnja, Irena
PY  - 2018
UR  - http://doi.wiley.com/10.1002/jnr.24224
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3010
AB  - Multiple sclerosis (MS) is a chronic, progressive disorder of the central nervous system (CNS) that affects more than two million people worldwide. Several animal models resemble MS pathology; the most employed are experimental autoimmune encephalomyelitis (EAE) and toxin- and/or virus-induced demyelination. In this review we will summarize our knowledge on the utility of different animal models in MS research. Although animal models cannot replicate the complexity and heterogeneity of the MS pathology, they have proved to be useful for the development of several drugs approved for treatment of MS patients. This review focuses on EAE because it represents both clinical and pathological features of MS. During the past decades, EAE has been effective in illuminating various pathological processes that occur during MS, including inflammation, CNS penetration, demyelination, axonopathy, and neuron loss mediated by immune cells.
T2  - Journal of Neuroscience Research
T1  - Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis.
IS  - 6
VL  - 96
DO  - 10.1002/jnr.24224
SP  - 1021
EP  - 1042
ER  - 

@article{
author = "Bjelobaba, Ivana and Begović-Kuprešanin, Vesna and Peković, Sanja and Lavrnja, Irena",
year = "2018",
abstract = "Multiple sclerosis (MS) is a chronic, progressive disorder of the central nervous system (CNS) that affects more than two million people worldwide. Several animal models resemble MS pathology; the most employed are experimental autoimmune encephalomyelitis (EAE) and toxin- and/or virus-induced demyelination. In this review we will summarize our knowledge on the utility of different animal models in MS research. Although animal models cannot replicate the complexity and heterogeneity of the MS pathology, they have proved to be useful for the development of several drugs approved for treatment of MS patients. This review focuses on EAE because it represents both clinical and pathological features of MS. During the past decades, EAE has been effective in illuminating various pathological processes that occur during MS, including inflammation, CNS penetration, demyelination, axonopathy, and neuron loss mediated by immune cells.",
journal = "Journal of Neuroscience Research",
title = "Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis.",
number = "6",
volume = "96",
doi = "10.1002/jnr.24224",
pages = "1021-1042"
}

Bjelobaba, I., Begović-Kuprešanin, V., Peković, S.,& Lavrnja, I.. (2018). Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis.. in Journal of Neuroscience Research, 96(6), 1021-1042.
https://doi.org/10.1002/jnr.24224

Bjelobaba I, Begović-Kuprešanin V, Peković S, Lavrnja I. Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis.. in Journal of Neuroscience Research. 2018;96(6):1021-1042.
doi:10.1002/jnr.24224 .

Bjelobaba, Ivana, Begović-Kuprešanin, Vesna, Peković, Sanja, Lavrnja, Irena, "Animal models of multiple sclerosis: Focus on experimental autoimmune encephalomyelitis." in Journal of Neuroscience Research, 96, no. 6 (2018):1021-1042,
https://doi.org/10.1002/jnr.24224 . .